Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0003268

Physical Location & Seq

Genomic Coordinates Forward (+)

19734624 .. 19739778

Gene Structure 5155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g26630.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2910 bp

ATGGATAGGATGTGGAGGGTAGGGAATTTGGTGTTTTTGTGTCTATTGGGTCTGGCTTATGGTGCTACTAACCCGGATGACATGAAGATTCTGAATGATTTTAGAAAAGGGTTGGAGAATCCAGAGCTTCTTGAATGGCCTGAAAATGGGGATGACCCTTGTGGTTCCAAATGGCCTCATGTGTTTTGTAGTGGGGATAGAGTTTCACAGATTCAAGTTCAGGGTATGGGGCTTAAAGGACCTCTGCCTCAGAACTTTAATCAGCTCTCACAGCTCTCCAACCTTGGTCTCCAAAGGAACAAGTTCAATGGCAAGTTGCCCACTTTTAGTGGCTTATCTGAACTGAAGTATGCTTATTTGGGGGACAATGATTTTGATACTATCCCGTCCGATTTCTTCAATGGCCTTAGCAGTCTCATGGTCATGGCTTTGGATCACAATCCGCTGAATGCGTCTACCGGGTGGTCTATTCCTGATGAGTTAGAAATGTCAGCTCAGCTACAAAATCTTTCCTTGATTGAATGCAACTTGGTTGGACAAGTGCCTGAATTCTTAGGCACATTGTCGAATCTCAAAATGCTGCAACTTTCCTATAACAGATTAACTGGTGGTTTGCCCAAGAGTTTTGGACAGTCTTTGTTGCAGATTTTGTGGTTGAATAATCAAGATGGTGGTGGGATGACTAGTCCAATTGATGTTATTGGATCAATGACGTCTTTGACACAGGTTTGGCTGCATGGAAACAAATTCACGGGGACAATACCAGAGAACATAGGAGGTCTTTTATCTCTTAAGGAACTCAACCTCAATGGTAACCAACTTGTTGGTCAGATTCCTCAAGCATTGGCTAAGTTGAAGCTAGACAAATTGGACCTGGCAAACAACCAGTTAATGGGTCCAATACCGAAGTTTCAGTCTGGTAATGTTTCCTATAGCTCTAATTCATTTTGTCAATCTGATCCTGGACTTCTATGCGCCCCACAAGTTACAGTACTTCTGGATTTTCTTGGTGATCTGAGCTACCCATCAAGTCTGACCTCTGACTGGTCTGGTAATGACCCCTGTGGGGGGCCATGGCTGGGAATAACTTGCAATCCAGAGTCGAAAGTTTCTGTCATCAATTTGCCTAAGCGCAATCTAACTGGTAAGCTGAGTCCTTCGCTTGTGAGTTTAGATTCTCTGGTTACTATTATGCTTGCTGGAAATGATATAAGTGGTACAGTTCCTTCTAATCTTACTGATTTGAAATCCTTGGCAAAATTGGATATAAGTGGAAACAATATTGAGCCTCCATTACCAAAATTTCGTGATAGTGTGACACTGATCACCGATGGAAATCCTCTCCTAGTTGCTAATAAAACAACCCCACCTCCTTTACCTACCAGAAGCCCACCCCCAACCCAAACACCAGCTCCAGAAGAAAATCCGCCACCAGGTACCACAACACCTCCTCAATCACCTGGTTCCCCTAGCCCACCTTCGCCAACTGAGACAAATGCAGTTCCACCTAAGTCTGAATCTCTCCTTAGAAGCTCCAAAACATCTAAACCAGTTATTGTTGTGGCTGGAGTTGCAATTATAGCTGTCTTCGCAATTCTTCTGTTGATGTGTCTATCTATATACTGGTGTAAGTACAGAAAAAGAATCTTGGAGGCTCCTTGTTCTATTGTGGTTCACCCTAGAGATCCATCTGATCCAGGAAACAAGCTTAAGGTTGCAGTTTCCAGCAATAATAATGGAATCTTATCAACCAAAACTGGGACTACTTATTTGAGTAACAACAGTAGTGGAACAGACAGCTCTCATATGATTGAGTCTGGTAACCTTGTCATATCTGTTCAAGTACTTCGAAAGGTGACCAATAACTTTGCACCTGAATATGAGCTAGGCCATGGTGGATTTGGAACTGTTTACAAGGGTGAACTAGAAGACGGGACAATTTTGGCTGTTAAGAGAATGGAGGGTGGTGTAATCAGCAGTAAAGGACTAGATGAATTTCAGGCAGAAATTGCTGTTCTTTCGAAGGTCCGTCATCGACATTTGGTTTCTTTGTTGGGTTACTCAGTCGAAGGGAGTGAAAGGCTTCTTGTCTATGAGTATATGTCTCAAGGCGCTCTTAGCAGGCATCTTTTCCATTGGAAGACCCTAAGTCTGAAGCCTTTGTCTTGGACAAGGAGACTTACAATTGCATTGGATGTTGCCAGAGGCATTGAGTATCTGCACAATTTAGCTCGCCAGACCTTCATCCACCGAGATCTCAAATCTTCTAATATTCTTCTGGGTGATGACTTCCACGCAAAAGTTTCTGATTTTGGATTGGTTAAACTTGCTCCAGATGGGGAGAAATCTTTTGCCACTAGGCTTGCCGGGACATTTGGATACCTTGCACCTGAATATGCAGTGATGGGGAAAATCACAACTAAGGTAGATGTGTTCAGCTTTGGCGTAGTGTTGATGGAGCTTGTGACTGGATTAATGGCACTGGATGAGAAACGGCCAGAGGAAAGTCGGTACTTGGCTGAGTGGTTTTGGCGAATCAAGTCGAGCAAGGAGACGCTTATGGCTGCAGTTGATCCAACTATTGAGGTTACTGAAGAAACTTTTGAGAGCATCTTGTTAATAGCTGAACTTGCTGGACATTGTACTGCGAGGGAGCCAACCCATCGGCCTGACATGGGACATGCAGTGAACGTGCTAGGCCCACTCGTTGAAAAGTGGAAACCATTTGATGAAGAATTAGAAAGTCTCTCTGGAATCGATTATAATCAACCTCTTTCCCAGATGCTAAAAGTTTGGCAGGAAGCGGAAAGCAGAGAGGTCAGCTATACTAGCCTGGAAGACAGCAAGGGAAGTATTCCTGCTAGACCTGCTGGATTTGCAGATTCCTTCACTTCTGCTGATGGTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

970

Amino Acids

105.52

Weight (kDa)

5.55

Isoelectric Point (pI)

39.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	164 - 284	3.9e-06	Leucine-rich repeat region
LRR_8	PF13855	239 - 297	1.2e-06	Leucine rich repeat
LRRNT_2	PF08263	327 - 365	3.2e-06	Leucine rich repeat N-terminal domain
Pkinase	PF00069	627 - 893	1.8e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	628 - 894	1.5e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003268)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g26630 FvH4_3g26630 FvH4_6g20300
malus_domestica	MD03G1154600.v1.1 MD12G1040200.v1.1 MD14G1038700.v1.1
prunus_persica	Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.7G094700_v2.0.a1
pyrus_communis	pycom03g10840 pycom12g03440 pycom14g03340
rosa_chinensis	RchiOBHm_Chr3g0475921 RchiOBHm_Chr5g0048201
rosa_laevigata	RLG00000023801 RLG00000034502
rosa_multiflora	Rmu_sc0001408.1_g000015 Rmu_sc0009168.1_g000004
rosa_roxburghii	Rroxscaffold_1G00033720 Rroxscaffold_6G00405730
rosa_rugosa	Rorug03G0151900 Rorug05G0237000 Rorug05G0237100
rosa_samantha	Rh3AG202700 Rh3BG232600 Rh3CG228000 Rh3DG228200 Rh5AG316200 Rh5BG326800 Rh5CG353000 Rh5DG337800
rosa_wichuraiana	Rw3G018440 Rw5G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2763
AatII	GACGTC	1 cut(s)	716
Acc36I	ACCTGC	1 cut(s)	2875
Acc65I	GGTACC	1 cut(s)	1436
AccB1I	GGYRCC	1 cut(s)	1436
AccB7I	CCANNNNNTGG	1 cut(s)	892
AccI	GTMKAC	1 cut(s)	455
AciI	CCGC	3 cut(s)	443, 1427, 2804
AclWI	GGATC	6 cut(s)	441, 712, 953, 1679, 1688, 2567
AcoI	YGGCCR	1 cut(s)	2495
AcsI	RAATTY	5 cut(s)	25, 548, 746, 1301, 1994
AcuI	CTGAAG	3 cut(s)	365, 2174, 2613
AcyI	GRCGYC	1 cut(s)	713
AfaI	GTAC	7 cut(s)	993, 1219, 1438, 1634, 1845, 2513, 2644
AflII	CTTAAG	2 cut(s)	791, 1709
AhdI	GACNNNNNGTC	1 cut(s)	2148
AhlI	ACTAGT	1 cut(s)	683
AjnI	CCWGG	6 cut(s)	873, 961, 1432, 1459, 1696, 2832
Alw26I	GTCTC	7 cut(s)	293, 419, 1484, 2109, 2170, 2546, 2750
AlwI	GGATC	6 cut(s)	441, 712, 953, 1679, 1688, 2567
AoxI	GGCC	8 cut(s)	137, 173, 403, 1070, 1888, 2495, 2666, 2698
ApeKI	GCWGC	3 cut(s)	580, 733, 2564
ApoI	RAATTY	5 cut(s)	25, 548, 746, 1301, 1994
ArsI	GACNNNNNNTTYG	6 cut(s)	356, 388, 899, 931, 1753, 1785
AseI	ATTAAT	1 cut(s)	2474
Asp700I	GAANNNNTTC	2 cut(s)	302, 2082
Asp718I	GGTACC	1 cut(s)	1436
AspLEI	GCGC	3 cut(s)	977, 1134, 2114
AspS9I	GGNCC	6 cut(s)	239, 871, 896, 1070, 2026, 2699
AsuC2I	CCSGG	3 cut(s)	74, 460, 2368
AsuHPI	GGTGA	7 cut(s)	1022, 1318, 1449, 1667, 1867, 1931, 2294
AsuII	TTCGAA	2 cut(s)	1849, 2021
AvaII	GGWCC	4 cut(s)	239, 871, 896, 2026
BanI	GGYRCC	1 cut(s)	1436
BarI	GAAGNNNNNNTAC	2 cut(s)	1210, 1242
BbsI	GAAGAC	4 cut(s)	1579, 1935, 2147, 2844
BbvI	GCAGC	3 cut(s)	567, 720, 2551
BccI	CCATC	9 cut(s)	662, 1033, 1325, 1696, 2330, 2398, 2449, 2670, 2894
BceAI	ACGGC	1 cut(s)	2510
BciT130I	CCWGG	6 cut(s)	875, 963, 1434, 1461, 1698, 2834
BciVI	GTATCC	1 cut(s)	2372
BclI	TGATCA	1 cut(s)	1323
BcnI	CCSGG	3 cut(s)	74, 460, 2368
BcoDI	GTCTC	7 cut(s)	293, 419, 1484, 2109, 2170, 2546, 2750
BcuI	ACTAGT	1 cut(s)	683
BfmI	CTRYAG	2 cut(s)	931, 2565
BfoI	RGCGCY	1 cut(s)	2115
BfrI	CTTAAG	2 cut(s)	791, 1709
BfuAI	ACCTGC	1 cut(s)	2875
BfuI	GTATCC	1 cut(s)	2372
BglII	AGATCT	1 cut(s)	2254
BisI	GCNGC	3 cut(s)	581, 734, 2565
BlpI	GCTNAGC	1 cut(s)	495
BlsI	GCNGC	3 cut(s)	582, 735, 2566
BmcAI	AGTACT	2 cut(s)	993, 1845
Bme1390I	CCNGG	9 cut(s)	74, 460, 875, 963, 1434, 1461, 1698, 2368, 2834
Bme18I	GGWCC	4 cut(s)	239, 871, 896, 2026
BmeRI	GACNNNNNGTC	1 cut(s)	2148
BmgT120I	GGNCC	6 cut(s)	239, 871, 896, 1070, 2026, 2699
BmiI	GGNNCC	7 cut(s)	166, 897, 1071, 1438, 1465, 1656, 2655
BmrFI	CCNGG	9 cut(s)	74, 460, 875, 963, 1434, 1461, 1698, 2368, 2834
BmrI	ACTGGG	1 cut(s)	1767
BmsI	GCATC	3 cut(s)	2134, 2619, 2772
BmuI	ACTGGG	1 cut(s)	1767
BpiI	GAAGAC	4 cut(s)	1579, 1935, 2147, 2844
BpmI	CTGGAG	3 cut(s)	1398, 1587, 2316
Bpu10I	CCTNAGC	2 cut(s)	407, 1128
Bpu1102I	GCTNAGC	1 cut(s)	495
Bpu14I	TTCGAA	2 cut(s)	1849, 2021
BpuEI	CTTGAG	2 cut(s)	822, 2091
BpuMI	CCSGG	3 cut(s)	74, 460, 2368
Bsa29I	ATCGAT	1 cut(s)	2757
BsaBI	GATNNNNATC	3 cut(s)	438, 1335, 2763
BsaHI	GRCGYC	1 cut(s)	713
BsaI	GGTCTC	1 cut(s)	293
BsaJI	CCNNGG	4 cut(s)	283, 1073, 1251, 1891
BsaXI	ACNNNNNCTCC	6 cut(s)	1354, 1384, 1396, 1426, 1432, 1462
Bse1I	ACTGG	9 cut(s)	610, 886, 1049, 1147, 1550, 1628, 1762, 2473, 2487
Bse8I	GATNNNNATC	3 cut(s)	438, 1335, 2763
BseBI	CCWGG	6 cut(s)	875, 963, 1434, 1461, 1698, 2834
BseCI	ATCGAT	1 cut(s)	2757
BseDI	CCNNGG	4 cut(s)	283, 1073, 1251, 1891
BseGI	GGATG	7 cut(s)	15, 82, 157, 684, 2200, 2244, 2491
BseJI	GATNNNNATC	3 cut(s)	438, 1335, 2763
BseMII	CTCAG	7 cut(s)	263, 509, 1007, 1142, 1479, 2076, 2511
BseNI	ACTGG	9 cut(s)	610, 886, 1049, 1147, 1550, 1628, 1762, 2473, 2487
BseRI	GAGGAG	1 cut(s)	1440
BseXI	GCAGC	3 cut(s)	567, 720, 2551
BseYI	CCCAGC	1 cut(s)	1078
BsgI	GTGCAG	1 cut(s)	2204
BshFI	GGCC	8 cut(s)	139, 175, 405, 1072, 1890, 2497, 2668, 2700
BshNI	GGYRCC	1 cut(s)	1436
BshVI	ATCGAT	1 cut(s)	2757
BsiSI	CCGG	3 cut(s)	74, 459, 2367
BslFI	GGGAC	6 cut(s)	377, 769, 1774, 1948, 2383, 2691
BsmAI	GTCTC	7 cut(s)	293, 419, 1484, 2109, 2170, 2546, 2750
BsmBI	CGTCTC	1 cut(s)	2546
BsmFI	GGGAC	6 cut(s)	377, 769, 1774, 1948, 2383, 2691
BsmI	GAATGC	2 cut(s)	454, 527
BsnI	GGCC	8 cut(s)	139, 175, 405, 1072, 1890, 2497, 2668, 2700
Bso31I	GGTCTC	1 cut(s)	293
Bsp119I	TTCGAA	2 cut(s)	1849, 2021
Bsp143I	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
Bsp1720I	GCTNAGC	1 cut(s)	495
Bsp19I	CCATGG	2 cut(s)	1073, 1891
BspACI	CCGC	3 cut(s)	443, 1427, 2804
BspANI	GGCC	8 cut(s)	139, 175, 405, 1072, 1890, 2497, 2668, 2700
BspCNI	CTCAG	7 cut(s)	262, 508, 1008, 1143, 1480, 2075, 2512
BspDI	ATCGAT	1 cut(s)	2757
BspLI	GGNNCC	7 cut(s)	166, 897, 1071, 1438, 1465, 1656, 2655
BspMAI	CTGCAG	1 cut(s)	2569
BspMI	ACCTGC	1 cut(s)	2875
BspPI	GGATC	6 cut(s)	441, 712, 953, 1679, 1688, 2567
BspT104I	TTCGAA	2 cut(s)	1849, 2021
BspT107I	GGYRCC	1 cut(s)	1436
BspTI	CTTAAG	2 cut(s)	791, 1709
BspTNI	GGTCTC	1 cut(s)	293
BsrI	ACTGG	9 cut(s)	610, 886, 1049, 1147, 1550, 1628, 1762, 2473, 2487
BssECI	CCNNGG	4 cut(s)	283, 1073, 1251, 1891
BssMI	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
BssNI	GRCGYC	1 cut(s)	713
BssT1I	CCWWGG	4 cut(s)	283, 1073, 1251, 1891
Bst2UI	CCWGG	6 cut(s)	875, 963, 1434, 1461, 1698, 2834
Bst4CI	ACNGT	5 cut(s)	633, 991, 1222, 1784, 1909
BstACI	GRCGYC	1 cut(s)	713
BstAFI	CTTAAG	2 cut(s)	791, 1709
BstAPI	GCANNNNNTGC	1 cut(s)	2009
BstBI	TTCGAA	2 cut(s)	1849, 2021
BstC8I	GCNNGC	4 cut(s)	1197, 2123, 2233, 2364
BstDSI	CCRYGG	2 cut(s)	1073, 1891
BstEII	GGTNACC	3 cut(s)	812, 1820, 1855
BstF5I	GGATG	7 cut(s)	15, 82, 157, 684, 2200, 2244, 2491
BstH2I	RGCGCY	1 cut(s)	2115
BstHHI	GCGC	3 cut(s)	977, 1134, 2114
BstKTI	GATC	9 cut(s)	436, 707, 961, 1015, 1326, 1687, 1696, 2257, 2575
BstMAI	GTCTC	7 cut(s)	293, 419, 1484, 2109, 2170, 2546, 2750
BstMBI	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
BstNI	CCWGG	6 cut(s)	875, 963, 1434, 1461, 1698, 2834
BstNSI	RCATGY	1 cut(s)	2684
BstPI	GGTNACC	3 cut(s)	812, 1820, 1855
BstSCI	CCNGG	9 cut(s)	72, 458, 873, 961, 1432, 1459, 1696, 2366, 2832
BstSFI	CTRYAG	2 cut(s)	931, 2565
BstV1I	GCAGC	3 cut(s)	567, 720, 2551
BstV2I	GAAGAC	4 cut(s)	1579, 1935, 2147, 2844
BstX2I	RGATCY	2 cut(s)	1684, 2254
BstXI	CCANNNNNNTGG	1 cut(s)	824
BstYI	RGATCY	2 cut(s)	1684, 2254
Bsu15I	ATCGAT	1 cut(s)	2757
BsuI	GTATCC	1 cut(s)	2372
BsuRI	GGCC	8 cut(s)	139, 175, 405, 1072, 1890, 2497, 2668, 2700
BsuTUI	ATCGAT	1 cut(s)	2757
BtgI	CCRYGG	2 cut(s)	1073, 1891
BtsCI	GGATG	7 cut(s)	15, 82, 157, 684, 2200, 2244, 2491
BtsI	GCAGTG	2 cut(s)	2406, 2691
BtsIMutI	CAGTG	4 cut(s)	1319, 2406, 2480, 2691
BveI	ACCTGC	1 cut(s)	2875
Cac8I	GCNNGC	4 cut(s)	1197, 2123, 2233, 2364
CfoI	GCGC	3 cut(s)	977, 1134, 2114
Cfr13I	GGNCC	6 cut(s)	239, 871, 896, 1070, 2026, 2699
ClaI	ATCGAT	1 cut(s)	2757
CseI	GACGC	2 cut(s)	441, 2563
CsiI	ACCWGGT	2 cut(s)	1432, 1459
Csp6I	GTAC	7 cut(s)	992, 1218, 1437, 1633, 1844, 2512, 2643
CspCI	CAANNNNNGTGG	4 cut(s)	1768, 1803, 2344, 2379
CviAII	CATG	9 cut(s)	82, 179, 418, 424, 737, 1074, 1892, 2674, 2681
CviQI	GTAC	7 cut(s)	992, 1218, 1437, 1633, 1844, 2512, 2643
DpnI	GATC	9 cut(s)	435, 706, 960, 1014, 1325, 1686, 1695, 2256, 2574
DpnII	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
DriI	GACNNNNNGTC	1 cut(s)	2148
EaeI	YGGCCR	1 cut(s)	2495
Eam1105I	GACNNNNNGTC	1 cut(s)	2148
EciI	GGCGGA	1 cut(s)	1416
Eco130I	CCWWGG	4 cut(s)	283, 1073, 1251, 1891
Eco31I	GGTCTC	1 cut(s)	293
Eco47I	GGWCC	4 cut(s)	239, 871, 896, 2026
Eco57I	CTGAAG	3 cut(s)	365, 2174, 2613
Eco91I	GGTNACC	3 cut(s)	812, 1820, 1855
EcoO109I	RGGNCCY	1 cut(s)	239
EcoO65I	GGTNACC	3 cut(s)	812, 1820, 1855
EcoRI	GAATTC	1 cut(s)	548
EcoRII	CCWGG	6 cut(s)	873, 961, 1432, 1459, 1696, 2832
EcoT14I	CCWWGG	4 cut(s)	283, 1073, 1251, 1891
ErhI	CCWWGG	4 cut(s)	283, 1073, 1251, 1891
Esp3I	CGTCTC	1 cut(s)	2546
FaeI	CATG	9 cut(s)	85, 182, 421, 427, 740, 1077, 1895, 2677, 2684
FalI	AAGNNNNNCTT	2 cut(s)	2100, 2132
FaqI	GGGAC	6 cut(s)	377, 769, 1774, 1948, 2383, 2691
FatI	CATG	9 cut(s)	81, 178, 417, 423, 736, 1073, 1891, 2673, 2680
FauNDI	CATATG	1 cut(s)	1806
FbaI	TGATCA	1 cut(s)	1323
FblI	GTMKAC	1 cut(s)	455
Fnu4HI	GCNGC	3 cut(s)	581, 734, 2565
FokI	GGATG	7 cut(s)	22, 89, 164, 691, 2207, 2231, 2498
Fsp4HI	GCNGC	3 cut(s)	581, 734, 2565
GlaI	GCGC	3 cut(s)	976, 1133, 2113
GluI	GCNGC	3 cut(s)	581, 734, 2565
GsaI	CCCAGC	1 cut(s)	1082
GsuI	CTGGAG	3 cut(s)	1398, 1587, 2316
HaeII	RGCGCY	1 cut(s)	2115
HaeIII	GGCC	8 cut(s)	139, 175, 405, 1072, 1890, 2497, 2668, 2700
HapII	CCGG	3 cut(s)	74, 459, 2367
HgaI	GACGC	2 cut(s)	441, 2563
HhaI	GCGC	3 cut(s)	977, 1134, 2114
Hin1I	GRCGYC	1 cut(s)	713
Hin1II	CATG	9 cut(s)	85, 182, 421, 427, 740, 1077, 1895, 2677, 2684
Hin6I	GCGC	3 cut(s)	975, 1132, 2112
HinP1I	GCGC	3 cut(s)	975, 1132, 2112
HindIII	AAGCTT	1 cut(s)	1706
HpaII	CCGG	3 cut(s)	74, 459, 2367
HphI	GGTGA	7 cut(s)	1022, 1318, 1449, 1667, 1867, 1931, 2294
Hpy166II	GTNNAC	5 cut(s)	456, 1675, 1912, 1922, 2689
Hpy8I	GTNNAC	5 cut(s)	456, 1675, 1912, 1922, 2689
HpyAV	CCTTC	7 cut(s)	1167, 1236, 1488, 2017, 2063, 2251, 2896
HpyCH4III	ACNGT	5 cut(s)	633, 991, 1222, 1784, 1909
HpyCH4IV	ACGT	2 cut(s)	713, 2691
HpySE526I	ACGT	2 cut(s)	713, 2691
Hsp92I	GRCGYC	1 cut(s)	713
Hsp92II	CATG	9 cut(s)	85, 182, 421, 427, 740, 1077, 1895, 2677, 2684
HspAI	GCGC	3 cut(s)	975, 1132, 2112
KpnI	GGTACC	1 cut(s)	1440
Ksp22I	TGATCA	1 cut(s)	1323
Kzo9I	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
LmnI	GCTCC	6 cut(s)	1417, 1538, 1660, 2335, 2458, 2653
Lsp1109I	GCAGC	3 cut(s)	567, 720, 2551
LweI	GCATC	3 cut(s)	2134, 2619, 2772
MabI	ACCWGGT	2 cut(s)	1432, 1459
MaeII	ACGT	2 cut(s)	713, 2691
MalI	GATC	9 cut(s)	435, 706, 960, 1014, 1325, 1686, 1695, 2256, 2574
MboI	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
MfeI	CAATTG	2 cut(s)	690, 2184
MflI	RGATCY	2 cut(s)	1684, 2254
MlyI	GAGTC	3 cut(s)	1109, 1162, 1823
MmeI	TCCRAC	4 cut(s)	93, 303, 514, 2600
MroXI	GAANNNNTTC	2 cut(s)	302, 2082
MslI	CAYNNNNRTG	1 cut(s)	422
MspA1I	CMGCKG	1 cut(s)	445
MspCI	CTTAAG	2 cut(s)	791, 1709
MspI	CCGG	3 cut(s)	74, 459, 2367
MspR9I	CCNGG	9 cut(s)	74, 460, 875, 963, 1434, 1461, 1698, 2368, 2834
MunI	CAATTG	2 cut(s)	690, 2184
Mva1269I	GAATGC	2 cut(s)	454, 527
MvaI	CCWGG	6 cut(s)	875, 963, 1434, 1461, 1698, 2834
NciI	CCSGG	3 cut(s)	74, 460, 2368
NcoI	CCATGG	2 cut(s)	1073, 1891
NdeI	CATATG	1 cut(s)	1806
NdeII	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
NlaIII	CATG	9 cut(s)	85, 182, 421, 427, 740, 1077, 1895, 2677, 2684
NlaIV	GGNNCC	7 cut(s)	166, 897, 1071, 1438, 1465, 1656, 2655
NmuCI	GTSAC	3 cut(s)	1315, 1855, 2464
NspI	RCATGY	1 cut(s)	2684
NspV	TTCGAA	2 cut(s)	1849, 2021
PctI	GAATGC	2 cut(s)	454, 527
PdmI	GAANNNNTTC	2 cut(s)	302, 2082
PflMI	CCANNNNNTGG	1 cut(s)	892
PfoI	TCCNGGA	2 cut(s)	961, 1696
PkrI	GCNGC	3 cut(s)	582, 735, 2566
PleI	GAGTC	3 cut(s)	1108, 1161, 1822
PpsI	GAGTC	3 cut(s)	1108, 1161, 1822
PpuMI	RGGWCCY	1 cut(s)	239
PshBI	ATTAAT	1 cut(s)	2474
PsiI	TTATAA	1 cut(s)	2763
Psp5II	RGGWCCY	1 cut(s)	239
Psp6I	CCWGG	6 cut(s)	873, 961, 1432, 1459, 1696, 2832
PspEI	GGTNACC	3 cut(s)	812, 1820, 1855
PspFI	CCCAGC	1 cut(s)	1078
PspGI	CCWGG	6 cut(s)	873, 961, 1432, 1459, 1696, 2832
PspN4I	GGNNCC	7 cut(s)	166, 897, 1071, 1438, 1465, 1656, 2655
PspPI	GGNCC	6 cut(s)	239, 871, 896, 1070, 2026, 2699
PspPPI	RGGWCCY	1 cut(s)	239
PstI	CTGCAG	1 cut(s)	2569
PsuI	RGATCY	2 cut(s)	1684, 2254
RsaI	GTAC	7 cut(s)	993, 1219, 1438, 1634, 1845, 2513, 2644
RsaNI	GTAC	7 cut(s)	992, 1218, 1437, 1633, 1844, 2512, 2643
RseI	CAYNNNNRTG	1 cut(s)	422
SatI	GCNGC	3 cut(s)	581, 734, 2565
Sau3AI	GATC	9 cut(s)	433, 704, 958, 1012, 1323, 1684, 1693, 2254, 2572
Sau96I	GGNCC	6 cut(s)	239, 871, 896, 1070, 2026, 2699
ScaI	AGTACT	2 cut(s)	993, 1845
SchI	GAGTC	3 cut(s)	1109, 1162, 1823
ScrFI	CCNGG	9 cut(s)	74, 460, 875, 963, 1434, 1461, 1698, 2368, 2834
SexAI	ACCWGGT	2 cut(s)	1432, 1459
SfaNI	GCATC	3 cut(s)	2134, 2619, 2772
SfcI	CTRYAG	2 cut(s)	931, 2565
SfuI	TTCGAA	2 cut(s)	1849, 2021
SinI	GGWCC	4 cut(s)	239, 871, 896, 2026
SmiMI	CAYNNNNRTG	1 cut(s)	422
SmlI	CTYRAG	4 cut(s)	791, 837, 1709, 2106
SmoI	CTYRAG	4 cut(s)	791, 837, 1709, 2106
SpeI	ACTAGT	1 cut(s)	683
SsiI	CCGC	3 cut(s)	443, 1427, 2804
SspI	AATATT	2 cut(s)	1282, 2272
StyD4I	CCNGG	9 cut(s)	72, 458, 873, 961, 1432, 1459, 1696, 2366, 2832
StyI	CCWWGG	4 cut(s)	283, 1073, 1251, 1891
TaaI	ACNGT	5 cut(s)	633, 991, 1222, 1784, 1909
TaiI	ACGT	2 cut(s)	716, 2694
TaqI	TCGA	9 cut(s)	566, 1103, 1849, 2021, 2035, 2067, 2543, 2757, 2905
TatI	WGTACW	4 cut(s)	991, 1632, 1843, 2642
TscAI	CASTG	4 cut(s)	1326, 2406, 2487, 2691
TseFI	GTSAC	3 cut(s)	1315, 1855, 2464
TseI	GCWGC	3 cut(s)	580, 733, 2564
Tsp45I	GTSAC	3 cut(s)	1315, 1855, 2464
TspDTI	ATGAA	5 cut(s)	98, 933, 2007, 2233, 2745
TspGWI	ACGGA	1 cut(s)	2018
TspRI	CASTG	4 cut(s)	1326, 2406, 2487, 2691
Van91I	CCANNNNNTGG	1 cut(s)	892
Vha464I	CTTAAG	2 cut(s)	791, 1709
VpaK11BI	GGWCC	4 cut(s)	239, 871, 896, 2026
VspI	ATTAAT	1 cut(s)	2474
XapI	RAATTY	5 cut(s)	25, 548, 746, 1301, 1994
XceI	RCATGY	1 cut(s)	2684
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1898
XmiI	GTMKAC	1 cut(s)	455
XmnI	GAANNNNTTC	2 cut(s)	302, 2082
ZraI	GACGTC	1 cut(s)	714
ZrmI	AGTACT	2 cut(s)	993, 1845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.