Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0003268

Physical Location & Seq

Genomic Coordinates Reverse (-)

46274375 .. 46278135

Gene Structure 3761 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG316200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2922 bp

ATGGATAGGAAGTGGATGGTAGGCAATTTCCTGTTTTTGTGGCTATTGGCTGTGGTTTATGGTGCCACTAACCCTGATGATATGAAGATTCTGAATGATTTGAGAAAAGGGTTGGAGAATCCAGAGCTTCTTAAATGGCCAGAAAATGGGGATGACCCTTGTGGCTCCAAATGGCCTCATGTGTTTTGTAGTGGGGATAGAGTTACACAGATTCAAGTTCAGGGTGTGGGGCTTAAAGGACCTCTGCCTCAGAACTTTAATCAGCTCTCAAAGCTCTACAACCTTGGTCTTCAAAAGAACAACTTCAATGGCAAGTTACCCACGTTTAGTGGCTTATCTGAACTGCAATATGCTTATTTGGATAACAATGATTTTGATACTATCCCGTCCGATTTCTTCAATGGCCTTACCAGTCTCTCAGTCATGGCTTTGGATAACATTCCGCTGAATGCGTCTACCGGGTGGTCTATTCCTAGTGAGTTAGAAAACTCAGCTCAGCTACAAAATCTTTCCTTGATTGAGTGTAACTTGGTTGGACAACTGCCTGAATTCTTAGGCACATTACCGAATCTCAGGATGCTGCAACTTTCGTTTAACAGATTAACTGGTGGTTTGCCCAAGAGTTTTGGACAGTCTTTGTTGCAGATTTTGTGGTTGAATAATCAAGATGGTGGTGGGATGACTGGTCCAATTGATGTTATTGGTTCAATGACGTCTTTGACACAGGTTTGGCTGCATGGAAACAAATTCACAGGGACAATACCAGAGAACATTGGAGATCTTGTATCTCTTAAGGAACTCAACCTCAATGGTAACCAACTTGTTGGTCAGATTCCTCAAGCATTGGCTGACTTGAAGCTCGACAAATTGGACCTGGCAAACAACCAGTTAATGGGTGCAGTACCCAAGTTTCAGTCTGGTAATGTTTCCTATAGTTCTAATTCATTTTGTCAAACTGATCCTGGGCTTCTATGTGCTCCACAAGTTATCGCACTTCTGGATTTTCTTGGTGATCTGAGTTACCCATCTAGTCTGACCTCTGACTGGTCTGGTAATGACCCCTGTGGGGGGCCATGGTTGGGAGTTAGTTGCAATCCGCAGTCGAAAGTTTCTGTCATCAATTTGCCTAGGCGCAAGCTAAATGGTACTCTGAGTCCTTCACTTGTGAATTTAGATTCTCTGATTGATATTAGGCTTGCTGGAAACAATATAAGAGGTACAGTTCCTACTAACCTCACTGAGTTGAAATCCTTGACGACATTGGATATAAGTGGAAACAATATTGAGCCTCCATTACCAAAATTTCGTGACAATGTGAGGCTCATCACCGATGGAAATCCTCTTCTAGTTGCTAATAAAACAGCCCCGTCTCCTTTACCTACCAGAAGCCCACCCCAACCCCAACCCCCACTCCAACCTCCAGCTCCAGTAGACAATCCGCCACCAGGTACCACAAAACCACCTCAATCACCAATTTCCCCTAGTCCACCTTCGCCAACTCACTCAAATGCAGGTGCACCTAAGTCGGAATCTCAACTCAAAAGCTCTAAAAGATCTAAACCAGTTATTGTTGTGGCTGCAGTTGCAGTTATAGCTGTATCTGTGATTCTTCTGTTGATGTGTCTATCTATATACTGGTGTAAGTACAGAAGAAACAACTTGGAGGCTCCTTGTTCCATTGTGGTTCACCCTAAAGACCCATCTGATCCAGAAAACAAGCTTAAGATTGCAGTTTCTAGCAATACAAACGGAAGCTTATCAACTAGAACTGGGACTACTTATTTGAGTAACAACAGCAGTGGAACACAAAGTTCTCAGATGATTGATTCTGGTAACCTTGTCATATCTGTTCAAGTTCTTCGAAAGGTGACCAATAACTTTGCACCTGAAAACGAGCTAGGCCATGGTGGATTTGGAACTGTTTACAAGGGTGAACTAGAAGATGGTACAAATCTGGCTGTTAAGAGAATGGAGGGTGGTGTAATCAGTAGTAAAGGATTGGATGAATTTCAGGCAGAAATTGCTGTACTTTCCAAAGTCCGTCATCGACATTTGGTTTCTCTGTTGGGGTACTCCGTTGAAGGGAGTGAAAGGCTTCTTGTCTATGAGTATATGTCTCAAGGCGCTCTTAGCAGGCATCTTTTCCATTGGAAGACCCTGAGTCTGAAGCCTTTGTCTTGGACAAGGAGACTTATAATTGCATTGGATGTTGCCAGAGGGATGGAGTATCTGCACAGCTTAGCTCGCCAGACCTTCATTCACAGAGATCTCAAATCTTCTAATATTCTTTTGGGTGATGACTTCCGTGCAAAAGTTTCGGATTTTGGATTGGTTAAACTTGCTCCAGATGGGGAGAAATCTGTTGCGACTAGGCTTGCTGGGACATTTGGATACCTTGCCCCTGAATATGCAGTGATGGGGAAAATCACAACTAAAGTAGATGTGTTCAGCTTTGGCGTAGTGTTAATGGAGCTTGTGACTGGATTAATGGCACTGGATGAGAACCGGCCTGAGGAAAGTCGTTACTTGGCTGAGTGGTTTTGGCGAATCAAGTCAAGCAAGGAGAAGCTTATGGCTGCAGTTGATCCAACTATTGAGGTTACTGAAGAAACTTTTGAGAGCATCTCCTTAATAGCTGATCTTGCTGGACATTGCACTGCGAGGGAGCCAACACATCGGCCTGACATGGGACATGCAGTGAACGTGCTAGGCCAACTTGTTGAAAAGTGGAAACCAGTTGATGAAGGATCAGAAAGTTTCTCTGGAATCGATTATAATCAACCTCTTTCCCAGATGCTAAAAGTTTGGCAGCAAGCAGAAAGCAGAGAGGTCAGCTATACTAGCCTGGAGGACAGCAAGGGAAGTATTCCTGCTAGACCCGCTGGATTTGCAGATTCCTTCACTTCTGCCGATGGTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

973

Amino Acids

106.08

Weight (kDa)

5.96

Isoelectric Point (pI)

38.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	159 - 282	4.5e-07	Leucine-rich repeat region
LRR_8	PF13855	239 - 297	7.9e-07	Leucine rich repeat
LRRNT_2	PF08263	327 - 365	2.8e-06	Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr	PF07714	630 - 900	2.1e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	630 - 900	2.1e-42	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003268)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g26630 FvH4_3g26630 FvH4_6g20300
malus_domestica	MD03G1154600.v1.1 MD12G1040200.v1.1 MD14G1038700.v1.1
prunus_persica	Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.7G094700_v2.0.a1
pyrus_communis	pycom03g10840 pycom12g03440 pycom14g03340
rosa_chinensis	RchiOBHm_Chr3g0475921 RchiOBHm_Chr5g0048201
rosa_laevigata	RLG00000023801 RLG00000034502
rosa_multiflora	Rmu_sc0001408.1_g000015 Rmu_sc0009168.1_g000004
rosa_roxburghii	Rroxscaffold_1G00033720 Rroxscaffold_6G00405730
rosa_rugosa	Rorug03G0151900 Rorug05G0237000 Rorug05G0237100
rosa_samantha	Rh3AG202700 Rh3BG232600 Rh3CG228000 Rh3DG228200 Rh5AG316200 Rh5BG326800 Rh5CG353000 Rh5DG337800
rosa_wichuraiana	Rw3G018440 Rw5G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2195, 2775
AarI	CACCTGC	1 cut(s)	1502
AatII	GACGTC	1 cut(s)	716
Acc36I	ACCTGC	1 cut(s)	1502
Acc65I	GGTACC	1 cut(s)	1448
AccB1I	GGYRCC	2 cut(s)	62, 1448
AccB7I	CCANNNNNTGG	2 cut(s)	146, 892
AccI	GTMKAC	2 cut(s)	455, 1431
AciI	CCGC	4 cut(s)	443, 1097, 1439, 2880
AclWI	GGATC	4 cut(s)	953, 1700, 2579, 2755
AcoI	YGGCCR	1 cut(s)	137
AcsI	RAATTY	5 cut(s)	548, 746, 1168, 1301, 2006
AcuI	CTGAAG	2 cut(s)	2186, 2625
AcyI	GRCGYC	1 cut(s)	713
AfaI	GTAC	8 cut(s)	903, 1147, 1219, 1450, 1646, 1948, 2028, 2072
AflII	CTTAAG	2 cut(s)	791, 1721
AhdI	GACNNNNNGTC	1 cut(s)	2160
AjnI	CCWGG	4 cut(s)	873, 961, 1444, 2844
Alw21I	GWGCWC	2 cut(s)	979, 1519
Alw26I	GTCTC	4 cut(s)	419, 1374, 2121, 2182
Alw44I	GTGCAC	1 cut(s)	1515
AlwI	GGATC	4 cut(s)	953, 1700, 2579, 2755
AoxI	GGCC	8 cut(s)	137, 173, 403, 1070, 1900, 2507, 2678, 2710
ApaLI	GTGCAC	1 cut(s)	1515
ApeKI	GCWGC	5 cut(s)	580, 733, 1577, 2576, 2809
ApoI	RAATTY	5 cut(s)	548, 746, 1168, 1301, 2006
ArsI	GACNNNNNNTTYG	2 cut(s)	1765, 1797
AseI	ATTAAT	1 cut(s)	2486
Asp700I	GAANNNNTTC	2 cut(s)	302, 2094
Asp718I	GGTACC	1 cut(s)	1448
AspA2I	CCTAGG	1 cut(s)	1127
AspLEI	GCGC	2 cut(s)	1134, 2126
AspS9I	GGNCC	4 cut(s)	239, 686, 871, 1070
AsuC2I	CCSGG	1 cut(s)	460
AsuHPI	GGTGA	7 cut(s)	1022, 1318, 1461, 1679, 1879, 1943, 2306
AsuII	TTCGAA	1 cut(s)	1861
AvaII	GGWCC	3 cut(s)	239, 686, 871
AvrII	CCTAGG	1 cut(s)	1127
AxyI	CCTNAGG	1 cut(s)	2511
BaeGI	GKGCMC	1 cut(s)	1519
BalI	TGGCCA	1 cut(s)	139
BanI	GGYRCC	2 cut(s)	62, 1448
BbsI	GAAGAC	2 cut(s)	281, 2159
Bbv12I	GWGCWC	2 cut(s)	979, 1519
BbvI	GCAGC	5 cut(s)	567, 720, 1564, 2563, 2821
BcgI	CGANNNNNNTGC	4 cut(s)	1506, 1540, 2298, 2332
BciT130I	CCWGG	4 cut(s)	875, 963, 1446, 2846
BciVI	GTATCC	1 cut(s)	2384
BcnI	CCSGG	1 cut(s)	460
BcoDI	GTCTC	4 cut(s)	419, 1374, 2121, 2182
BfmI	CTRYAG	3 cut(s)	931, 1578, 2577
BfoI	RGCGCY	1 cut(s)	2127
BfrI	CTTAAG	2 cut(s)	791, 1721
BfuAI	ACCTGC	1 cut(s)	1502
BfuI	GTATCC	1 cut(s)	2384
BglII	AGATCT	3 cut(s)	778, 1553, 2266
BisI	GCNGC	5 cut(s)	581, 734, 1578, 2577, 2810
BlnI	CCTAGG	1 cut(s)	1127
BlpI	GCTNAGC	2 cut(s)	495, 2239
BlsI	GCNGC	5 cut(s)	582, 735, 1579, 2578, 2811
Bme1390I	CCNGG	5 cut(s)	460, 875, 963, 1446, 2846
Bme18I	GGWCC	3 cut(s)	239, 686, 871
BmeRI	GACNNNNNGTC	1 cut(s)	2160
BmgT120I	GGNCC	4 cut(s)	239, 686, 871, 1070
BmiI	GGNNCC	6 cut(s)	64, 166, 1071, 1450, 1668, 2667
BmrFI	CCNGG	5 cut(s)	460, 875, 963, 1446, 2846
BmrI	ACTGGG	1 cut(s)	1779
BmsI	GCATC	4 cut(s)	567, 2146, 2631, 2784
BmuI	ACTGGG	1 cut(s)	1779
BpiI	GAAGAC	2 cut(s)	281, 2159
BplI	GAGNNNNNCTC	2 cut(s)	2609, 2641
BpmI	CTGGAG	4 cut(s)	1404, 1410, 2328, 2867
Bpu1102I	GCTNAGC	2 cut(s)	495, 2239
Bpu14I	TTCGAA	1 cut(s)	1861
BpuEI	CTTGAG	2 cut(s)	822, 2103
BpuMI	CCSGG	1 cut(s)	460
Bsa29I	ATCGAT	1 cut(s)	2769
BsaBI	GATNNNNATC	2 cut(s)	1335, 2775
BsaHI	GRCGYC	1 cut(s)	713
BsaJI	CCNNGG	5 cut(s)	283, 962, 1073, 1127, 1903
BsaXI	ACNNNNNCTCC	4 cut(s)	768, 798, 1395, 1425
Bse118I	RCCGGY	1 cut(s)	2505
Bse21I	CCTNAGG	1 cut(s)	2511
Bse3DI	GCAATG	1 cut(s)	2650
Bse8I	GATNNNNATC	2 cut(s)	1335, 2775
BseBI	CCWGG	4 cut(s)	875, 963, 1446, 2846
BseCI	ATCGAT	1 cut(s)	2769
BseDI	CCNNGG	5 cut(s)	283, 962, 1073, 1127, 1903
BseGI	GGATG	8 cut(s)	21, 157, 582, 684, 2008, 2212, 2226, 2503
BseJI	GATNNNNATC	2 cut(s)	1335, 2775
BseMI	GCAATG	1 cut(s)	2650
BseSI	GKGCMC	1 cut(s)	1519
BseXI	GCAGC	5 cut(s)	567, 720, 1564, 2563, 2821
BseYI	CCCAGC	1 cut(s)	2378
BsgI	GTGCAG	2 cut(s)	918, 2216
BshFI	GGCC	8 cut(s)	139, 175, 405, 1072, 1902, 2509, 2680, 2712
BshNI	GGYRCC	2 cut(s)	62, 1448
BshVI	ATCGAT	1 cut(s)	2769
BsiHKAI	GWGCWC	2 cut(s)	979, 1519
BsiSI	CCGG	2 cut(s)	459, 2506
BslFI	GGGAC	4 cut(s)	769, 1786, 2395, 2703
BsmAI	GTCTC	4 cut(s)	419, 1374, 2121, 2182
BsmBI	CGTCTC	1 cut(s)	1374
BsmFI	GGGAC	4 cut(s)	769, 1786, 2395, 2703
BsmI	GAATGC	1 cut(s)	454
BsnI	GGCC	8 cut(s)	139, 175, 405, 1072, 1902, 2509, 2680, 2712
Bsp119I	TTCGAA	1 cut(s)	1861
Bsp1286I	GDGCHC	2 cut(s)	979, 1519
Bsp143I	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
Bsp1720I	GCTNAGC	2 cut(s)	495, 2239
Bsp19I	CCATGG	2 cut(s)	1073, 1903
BspACI	CCGC	4 cut(s)	443, 1097, 1439, 2880
BspANI	GGCC	8 cut(s)	139, 175, 405, 1072, 1902, 2509, 2680, 2712
BspDI	ATCGAT	1 cut(s)	2769
BspLI	GGNNCC	6 cut(s)	64, 166, 1071, 1450, 1668, 2667
BspMAI	CTGCAG	2 cut(s)	1582, 2581
BspMI	ACCTGC	1 cut(s)	1502
BspPI	GGATC	4 cut(s)	953, 1700, 2579, 2755
BspT104I	TTCGAA	1 cut(s)	1861
BspT107I	GGYRCC	2 cut(s)	62, 1448
BspTI	CTTAAG	2 cut(s)	791, 1721
BsrDI	GCAATG	1 cut(s)	2650
BsrFI	RCCGGY	1 cut(s)	2505
BssAI	RCCGGY	1 cut(s)	2505
BssECI	CCNNGG	5 cut(s)	283, 962, 1073, 1127, 1903
BssMI	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
BssNI	GRCGYC	1 cut(s)	713
BssT1I	CCWWGG	4 cut(s)	283, 1073, 1127, 1903
Bst2UI	CCWGG	4 cut(s)	875, 963, 1446, 2846
Bst4CI	ACNGT	3 cut(s)	633, 1222, 1921
Bst6I	CTCTTC	1 cut(s)	1347
BstACI	GRCGYC	1 cut(s)	713
BstAFI	CTTAAG	2 cut(s)	791, 1721
BstAPI	GCANNNNNTGC	1 cut(s)	2021
BstBI	TTCGAA	1 cut(s)	1861
BstC8I	GCNNGC	6 cut(s)	1136, 1197, 2135, 2245, 2376, 2814
BstDSI	CCRYGG	2 cut(s)	1073, 1903
BstEII	GGTNACC	3 cut(s)	812, 1832, 1867
BstF5I	GGATG	8 cut(s)	21, 157, 582, 684, 2008, 2212, 2226, 2503
BstH2I	RGCGCY	1 cut(s)	2127
BstHHI	GCGC	2 cut(s)	1134, 2126
BstKTI	GATC	9 cut(s)	781, 961, 1015, 1556, 1708, 2269, 2587, 2641, 2750
BstMAI	GTCTC	4 cut(s)	419, 1374, 2121, 2182
BstMBI	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
BstNI	CCWGG	4 cut(s)	875, 963, 1446, 2846
BstNSI	RCATGY	1 cut(s)	2696
BstPI	GGTNACC	3 cut(s)	812, 1832, 1867
BstSCI	CCNGG	5 cut(s)	458, 873, 961, 1444, 2844
BstSFI	CTRYAG	3 cut(s)	931, 1578, 2577
BstSLI	GKGCMC	1 cut(s)	1519
BstV1I	GCAGC	5 cut(s)	567, 720, 1564, 2563, 2821
BstV2I	GAAGAC	2 cut(s)	281, 2159
BstX2I	RGATCY	3 cut(s)	778, 1553, 2266
BstXI	CCANNNNNNTGG	2 cut(s)	824, 2221
BstYI	RGATCY	3 cut(s)	778, 1553, 2266
Bsu15I	ATCGAT	1 cut(s)	2769
Bsu36I	CCTNAGG	1 cut(s)	2511
BsuI	GTATCC	1 cut(s)	2384
BsuRI	GGCC	8 cut(s)	139, 175, 405, 1072, 1902, 2509, 2680, 2712
BsuTUI	ATCGAT	1 cut(s)	2769
BtgI	CCRYGG	2 cut(s)	1073, 1903
BtsCI	GGATG	8 cut(s)	21, 157, 582, 684, 2008, 2212, 2226, 2503
BtsI	GCAGTG	4 cut(s)	1804, 2418, 2655, 2703
BtsIMutI	CAGTG	6 cut(s)	1236, 1804, 2418, 2492, 2655, 2703
BveI	ACCTGC	1 cut(s)	1502
Cac8I	GCNNGC	6 cut(s)	1136, 1197, 2135, 2245, 2376, 2814
CfoI	GCGC	2 cut(s)	1134, 2126
Cfr10I	RCCGGY	1 cut(s)	2505
Cfr13I	GGNCC	4 cut(s)	239, 686, 871, 1070
ClaI	ATCGAT	1 cut(s)	2769
CseI	GACGC	1 cut(s)	441
CsiI	ACCWGGT	1 cut(s)	1444
Csp6I	GTAC	8 cut(s)	902, 1146, 1218, 1449, 1645, 1947, 2027, 2071
CspCI	CAANNNNNGTGG	2 cut(s)	1780, 1815
CviAII	CATG	7 cut(s)	179, 424, 737, 1074, 1904, 2686, 2693
CviQI	GTAC	8 cut(s)	902, 1146, 1218, 1449, 1645, 1947, 2027, 2071
DpnI	GATC	9 cut(s)	780, 960, 1014, 1555, 1707, 2268, 2586, 2640, 2749
DpnII	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
DriI	GACNNNNNGTC	1 cut(s)	2160
EaeI	YGGCCR	1 cut(s)	137
Eam1104I	CTCTTC	1 cut(s)	1347
Eam1105I	GACNNNNNGTC	1 cut(s)	2160
EarI	CTCTTC	1 cut(s)	1347
EciI	GGCGGA	1 cut(s)	1428
Eco130I	CCWWGG	4 cut(s)	283, 1073, 1127, 1903
Eco47I	GGWCC	3 cut(s)	239, 686, 871
Eco57I	CTGAAG	2 cut(s)	2186, 2625
Eco81I	CCTNAGG	1 cut(s)	2511
Eco91I	GGTNACC	3 cut(s)	812, 1832, 1867
EcoO109I	RGGNCCY	1 cut(s)	239
EcoO65I	GGTNACC	3 cut(s)	812, 1832, 1867
EcoRI	GAATTC	1 cut(s)	548
EcoRII	CCWGG	4 cut(s)	873, 961, 1444, 2844
EcoT14I	CCWWGG	4 cut(s)	283, 1073, 1127, 1903
ErhI	CCWWGG	4 cut(s)	283, 1073, 1127, 1903
Esp3I	CGTCTC	1 cut(s)	1374
FaeI	CATG	7 cut(s)	182, 427, 740, 1077, 1907, 2689, 2696
FalI	AAGNNNNNCTT	4 cut(s)	287, 319, 2112, 2144
FaqI	GGGAC	4 cut(s)	769, 1786, 2395, 2703
FatI	CATG	7 cut(s)	178, 423, 736, 1073, 1903, 2685, 2692
FauI	CCCGC	1 cut(s)	2887
FblI	GTMKAC	2 cut(s)	455, 1431
Fnu4HI	GCNGC	5 cut(s)	581, 734, 1578, 2577, 2810
FokI	GGATG	8 cut(s)	28, 164, 589, 691, 2015, 2219, 2233, 2510
Fsp4HI	GCNGC	5 cut(s)	581, 734, 1578, 2577, 2810
GlaI	GCGC	2 cut(s)	1133, 2125
GluI	GCNGC	5 cut(s)	581, 734, 1578, 2577, 2810
GsaI	CCCAGC	1 cut(s)	2382
GsuI	CTGGAG	4 cut(s)	1404, 1410, 2328, 2867
HaeII	RGCGCY	1 cut(s)	2127
HaeIII	GGCC	8 cut(s)	139, 175, 405, 1072, 1902, 2509, 2680, 2712
HapII	CCGG	2 cut(s)	459, 2506
HgaI	GACGC	1 cut(s)	441
HhaI	GCGC	2 cut(s)	1134, 2126
Hin1I	GRCGYC	1 cut(s)	713
Hin1II	CATG	7 cut(s)	182, 427, 740, 1077, 1907, 2689, 2696
Hin6I	GCGC	2 cut(s)	1132, 2124
HinP1I	GCGC	2 cut(s)	1132, 2124
HindIII	AAGCTT	3 cut(s)	1718, 1753, 2567
HpaII	CCGG	2 cut(s)	459, 2506
HphI	GGTGA	7 cut(s)	1022, 1318, 1461, 1679, 1879, 1943, 2306
Hpy166II	GTNNAC	8 cut(s)	456, 1432, 1487, 1517, 1687, 1924, 1934, 2701
Hpy188III	TCNNGA	8 cut(s)	122, 574, 665, 998, 1307, 1709, 2345, 2763
Hpy8I	GTNNAC	8 cut(s)	456, 1432, 1487, 1517, 1687, 1924, 1934, 2701
HpyAV	CCTTC	6 cut(s)	1167, 1500, 2075, 2263, 2738, 2908
HpyCH4III	ACNGT	3 cut(s)	633, 1222, 1921
HpyCH4IV	ACGT	3 cut(s)	323, 713, 2703
HpySE526I	ACGT	3 cut(s)	323, 713, 2703
Hsp92I	GRCGYC	1 cut(s)	713
Hsp92II	CATG	7 cut(s)	182, 427, 740, 1077, 1907, 2689, 2696
HspAI	GCGC	2 cut(s)	1132, 2124
KpnI	GGTACC	1 cut(s)	1452
Kzo9I	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
LmnI	GCTCC	7 cut(s)	170, 982, 1429, 1672, 2347, 2470, 2665
Lsp1109I	GCAGC	5 cut(s)	567, 720, 1564, 2563, 2821
LweI	GCATC	4 cut(s)	567, 2146, 2631, 2784
MabI	ACCWGGT	1 cut(s)	1444
MaeII	ACGT	3 cut(s)	323, 713, 2703
MalI	GATC	9 cut(s)	780, 960, 1014, 1555, 1707, 2268, 2586, 2640, 2749
MboI	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
MfeI	CAATTG	1 cut(s)	690
MflI	RGATCY	3 cut(s)	778, 1553, 2266
MhlI	GDGCHC	2 cut(s)	979, 1519
MlsI	TGGCCA	1 cut(s)	139
MluNI	TGGCCA	1 cut(s)	139
MlyI	GAGTC	2 cut(s)	1162, 2170
MmeI	TCCRAC	5 cut(s)	93, 514, 1438, 1506, 2612
Mox20I	TGGCCA	1 cut(s)	139
MroXI	GAANNNNTTC	2 cut(s)	302, 2094
MscI	TGGCCA	1 cut(s)	139
MslI	CAYNNNNRTG	1 cut(s)	1506
Msp20I	TGGCCA	1 cut(s)	139
MspA1I	CMGCKG	2 cut(s)	445, 2882
MspCI	CTTAAG	2 cut(s)	791, 1721
MspI	CCGG	2 cut(s)	459, 2506
MspR9I	CCNGG	5 cut(s)	460, 875, 963, 1446, 2846
MunI	CAATTG	1 cut(s)	690
Mva1269I	GAATGC	1 cut(s)	454
MvaI	CCWGG	4 cut(s)	875, 963, 1446, 2846
NciI	CCSGG	1 cut(s)	460
NcoI	CCATGG	2 cut(s)	1073, 1903
NdeII	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
NlaIII	CATG	7 cut(s)	182, 427, 740, 1077, 1907, 2689, 2696
NlaIV	GGNNCC	6 cut(s)	64, 166, 1071, 1450, 1668, 2667
NmuCI	GTSAC	3 cut(s)	1307, 1867, 2476
NspI	RCATGY	1 cut(s)	2696
NspV	TTCGAA	1 cut(s)	1861
PaqCI	CACCTGC	1 cut(s)	1502
PctI	GAATGC	1 cut(s)	454
PdmI	GAANNNNTTC	2 cut(s)	302, 2094
PflMI	CCANNNNNTGG	2 cut(s)	146, 892
PkrI	GCNGC	5 cut(s)	582, 735, 1579, 2578, 2811
PleI	GAGTC	2 cut(s)	1161, 2169
PpsI	GAGTC	2 cut(s)	1161, 2169
PpuMI	RGGWCCY	1 cut(s)	239
PshBI	ATTAAT	1 cut(s)	2486
PsiI	TTATAA	2 cut(s)	2195, 2775
Psp5II	RGGWCCY	1 cut(s)	239
Psp6I	CCWGG	4 cut(s)	873, 961, 1444, 2844
PspEI	GGTNACC	3 cut(s)	812, 1832, 1867
PspFI	CCCAGC	1 cut(s)	2378
PspGI	CCWGG	4 cut(s)	873, 961, 1444, 2844
PspN4I	GGNNCC	6 cut(s)	64, 166, 1071, 1450, 1668, 2667
PspPI	GGNCC	4 cut(s)	239, 686, 871, 1070
PspPPI	RGGWCCY	1 cut(s)	239
PsrI	GAACNNNNNNTAC	2 cut(s)	1759, 1791
PstI	CTGCAG	2 cut(s)	1582, 2581
PsuI	RGATCY	3 cut(s)	778, 1553, 2266
RsaI	GTAC	8 cut(s)	903, 1147, 1219, 1450, 1646, 1948, 2028, 2072
RsaNI	GTAC	8 cut(s)	902, 1146, 1218, 1449, 1645, 1947, 2027, 2071
RseI	CAYNNNNRTG	1 cut(s)	1506
SatI	GCNGC	5 cut(s)	581, 734, 1578, 2577, 2810
Sau3AI	GATC	9 cut(s)	778, 958, 1012, 1553, 1705, 2266, 2584, 2638, 2747
Sau96I	GGNCC	4 cut(s)	239, 686, 871, 1070
SchI	GAGTC	2 cut(s)	1162, 2170
ScrFI	CCNGG	5 cut(s)	460, 875, 963, 1446, 2846
SduI	GDGCHC	2 cut(s)	979, 1519
SexAI	ACCWGGT	1 cut(s)	1444
SfaNI	GCATC	4 cut(s)	567, 2146, 2631, 2784
SfcI	CTRYAG	3 cut(s)	931, 1578, 2577
SfuI	TTCGAA	1 cut(s)	1861
SinI	GGWCC	3 cut(s)	239, 686, 871
SmiMI	CAYNNNNRTG	1 cut(s)	1506
SmlI	CTYRAG	4 cut(s)	791, 837, 1721, 2118
SmoI	CTYRAG	4 cut(s)	791, 837, 1721, 2118
SsiI	CCGC	4 cut(s)	443, 1097, 1439, 2880
SspI	AATATT	2 cut(s)	1282, 2284
StyD4I	CCNGG	5 cut(s)	458, 873, 961, 1444, 2844
StyI	CCWWGG	4 cut(s)	283, 1073, 1127, 1903
TaaI	ACNGT	3 cut(s)	633, 1222, 1921
TaiI	ACGT	3 cut(s)	326, 716, 2706
TaqI	TCGA	6 cut(s)	861, 1103, 1861, 2047, 2769, 2917
TatI	WGTACW	2 cut(s)	1644, 2026
TscAI	CASTG	6 cut(s)	1243, 1804, 2418, 2499, 2662, 2703
TseFI	GTSAC	3 cut(s)	1307, 1867, 2476
TseI	GCWGC	5 cut(s)	580, 733, 1577, 2576, 2809
Tsp45I	GTSAC	3 cut(s)	1307, 1867, 2476
TspDTI	ATGAA	5 cut(s)	98, 933, 2019, 2245, 2757
TspGWI	ACGGA	4 cut(s)	1764, 2030, 2065, 2294
TspRI	CASTG	6 cut(s)	1243, 1804, 2418, 2499, 2662, 2703
Van91I	CCANNNNNTGG	2 cut(s)	146, 892
Vha464I	CTTAAG	2 cut(s)	791, 1721
VneI	GTGCAC	1 cut(s)	1515
VpaK11BI	GGWCC	3 cut(s)	239, 686, 871
VspI	ATTAAT	1 cut(s)	2486
XapI	RAATTY	5 cut(s)	548, 746, 1168, 1301, 2006
XceI	RCATGY	1 cut(s)	2696
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1910
XmaJI	CCTAGG	1 cut(s)	1127
XmiI	GTMKAC	2 cut(s)	455, 1431
XmnI	GAANNNNTTC	2 cut(s)	302, 2094
ZraI	GACGTC	1 cut(s)	714

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.