Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0003268

Physical Location & Seq

Genomic Coordinates Reverse (-)

10174951 .. 10177296

Gene Structure 2346 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g10840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2346 bp

ATGGAGAAAATCTGGAGAAGGAGGAGGATATGCATTTTTGTAATTTTTTGTGTTTCCATGGTGGCTTATGGAGCCACTTTCCCAGGCGACCTGAAAATTCTGAATGATTTCAGAAAAGGGTTGGAGAATTCAGAGCTTTTGGAATGGCCAGACAATGGAAGTGACCCTTGTGGCCCTCCTTTATGGCCTCATGTGTACTGCAATGGGGACAGAGTCTCACAGATTCAGGTTCAGAAAATGGGACTCAAAGGTCCTTTGCCTCAGAACTTCAACCAGCTCTCTAAGCTCAGCAACCTTGGCTTCCAAAGGAACAACTTCAATGGGAAATTGCCTACCTTTAGTGGCTTATCAGAGCTCGAATTCGCTTACTTGGATAATAATGATTTCGATACAATCCCTTTCGATTTCTTTGATGGCCTTAGCAGTCTTAGGGTTATGGCATTGGATCACATTCCGTTGAATGCGTCTACTGGATGGTCTTTGCCTAGTGAGTTGGCAAAATCTGTTCAATTGCAAAATTTGTCCTTGATTGAATGTAATTTGGCAGGATCGTTGCCGGAGTTTTTAGGGGCATTGCCGTCTCTCTCAGTCCTGCAACTTTCCTTTAATAGATTAACTGGTGTGATTCCGGCGAGTTTTGGAGAGTCTTTGGTGCAGACATTGTGGTTGAACAATCAAGATGGTGGATTGACCGGTCCTATTGATGTAATTGCATCAATGTCGTCGTTGACACAGGTTTGGCTGCATGGAAACCAATTCACCGGAACAATTCCAGCCAACATTGGAGATCTTTCTTCTCTCAAGGAACTCAACCTCAATGGCAACCAACTTGTTGGTCTGATTCCGCAGTCACTGGCGGACATGAAGCTTGACAAATTGGACCTGGGAAATAACCGGCTAATGGGTACAATACCGAAGTTTAAGTCCGGTAATGTTTCTTATAACTCTAATCCATTTTGTCAGACTGATCCTGGGCTGCAATGTGCCCCAGAAGTTATTGCACTTTTGGATTTTCTTGGTGATTTGAATTATCCATTAAGTCTTGCATCTGAGTGGTCGGGTAATAATCCGTGTGAGGGGCCTTGGTTGGGGTTGAGTTGCAATCCTCAGACCAAGGTTTCCGTCATCAATTTGCCTAGACGCAAGCTAATTGGTACTTTGAATCCTTCACTTGCAAAGTTAAATTCTTTGATTGATATAAGGCTTCCAGGAAACAACATAAGTGGTAAAGTTCCTACTAACTTTACTGAGTTGAAATCCTTGAGATTATTGGATATAAGCGGAAACAATATCGAGCCTCCTTTACCAAAATTCCATGACAGTCTGAAGGTCATCATCGACGGAAATCCTCTTTTAGTTGCTAATCAAACTGTTGCCCCTCCTCCTTTATCTAGAAGCCCACCTCCACTCAATAGTTCACAACCTCCATCAGACAGTCCATCAGATACCCCATTATCCAGTGGTGGAAAAGCACCATTATCCCATAGTCCACCCTCACCAATTACACACTCGAGTCCTAATCCATCTGGTTCTGTCCAAGTGGATGTTCAGCCCCAAAATTCTAAGAAATCAAAAACAGTTATTATTGTGGCTGGAATCGCAGTTGTTTCTGTTGTGGCTCTTCTGATGATTTGTCTATTTATTTACTGCTGTAAGAATAGGAAAAAAATCTCGGAGGTTCCTACTTCCATTGTGCTTCACCCTAGAGACCCATCTGATCCAGAAAATATGTATAAGATTGCAGTTTCTAGTAATACCACTGGAAGCTTGTCAACTAAAACTGGGATTACTGCTAGTAACTTCAGCAGTGGAACAAAGAACTCTCATATGATTGAGGATGGGAACCTTGTCATATCTGTTCAAGTTCTTAGAAAGGTGACCAAAGATTTTGCACCCGAAAATGAGCTAGGCCGTGGCGGATTTGGAACTGTTTACAAGGGTGAACTAGAAGATGGGACAAAAATAGCAGTTAAGAGAATGGAGGGTGGGGTAATCAGTAGCAAAGCATTGGATGAATTTCAGTCAGAAATTGCTGTTCTTTCCAAGGTTCGTCATCGACATTTGGTTTCTCTTTTGGGTTACTCCGTCGAAGGAAATGAAAGGCTTCTTGTCTACGAGTATATGTCTCAAGGTGCTCTTAGCAGGCATCTTTTTCATTGGAAGAGCTTCAATCTGAAGCCTTTATCTTGGACAAGGAGACTCAGTATTGTGTTGGATGTTGCAAGAGCGATGGAGTACCTACATAATTTAGCCCGCCAGACATTCATACACCGAGATCGATCAAATCTTCTAATATTCTTTTGGATGATAACTTTCATGCAAAAGTTTCAGATTTTGGGTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

782

Amino Acids

85.66

Weight (kDa)

8.45

Isoelectric Point (pI)

38.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	141 - 274	3.7e-06	Leucine-rich repeat region
LRR_8	PF13855	242 - 300	1.2e-07	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	633 - 759	1.4e-22	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	633 - 765	1.2e-18	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003268)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g26630 FvH4_3g26630 FvH4_6g20300
malus_domestica	MD03G1154600.v1.1 MD12G1040200.v1.1 MD14G1038700.v1.1
prunus_persica	Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.7G094700_v2.0.a1
pyrus_communis	pycom03g10840 pycom12g03440 pycom14g03340
rosa_chinensis	RchiOBHm_Chr3g0475921 RchiOBHm_Chr5g0048201
rosa_laevigata	RLG00000023801 RLG00000034502
rosa_multiflora	Rmu_sc0001408.1_g000015 Rmu_sc0009168.1_g000004
rosa_roxburghii	Rroxscaffold_1G00033720 Rroxscaffold_6G00405730
rosa_rugosa	Rorug03G0151900 Rorug05G0237000 Rorug05G0237100
rosa_samantha	Rh3AG202700 Rh3BG232600 Rh3CG228000 Rh3DG228200 Rh5AG316200 Rh5BG326800 Rh5CG353000 Rh5DG337800
rosa_wichuraiana	Rw3G018440 Rw5G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	942
AasI	GACNNNNNNGTC	1 cut(s)	249
AccB7I	CCANNNNNTGG	1 cut(s)	155
AccI	GTMKAC	2 cut(s)	467, 2112
AciI	CCGC	5 cut(s)	845, 857, 1281, 1917, 2254
AclWI	GGATC	4 cut(s)	453, 556, 962, 1712
AcoI	YGGCCR	1 cut(s)	146
AcsI	RAATTY	8 cut(s)	96, 127, 359, 517, 1183, 1310, 1558, 2015
AcuI	CTGAAG	3 cut(s)	1346, 1786, 2195
AfaI	GTAC	4 cut(s)	197, 907, 1156, 2237
AfiI	CCNNNNNNNGG	4 cut(s)	155, 901, 1076, 1088
AgeI	ACCGGT	1 cut(s)	692
AjnI	CCWGG	4 cut(s)	82, 882, 970, 1207
AjuI	GAANNNNNNNTTGG	2 cut(s)	1530, 1562
AluBI	AGCT	9 cut(s)	136, 277, 286, 355, 868, 1147, 1767, 1906, 2166
AluI	AGCT	9 cut(s)	136, 277, 286, 355, 868, 1147, 1767, 1906, 2166
Alw21I	GWGCWC	2 cut(s)	357, 2137
Alw26I	GTCTC	5 cut(s)	220, 585, 1701, 2130, 2191
AlwI	GGATC	4 cut(s)	453, 556, 962, 1712
AlwNI	CAGNNNCTG	1 cut(s)	853
Ama87I	CYCGRG	1 cut(s)	1510
AoxI	GGCC	6 cut(s)	146, 172, 185, 415, 1079, 1909
ApeKI	GCWGC	2 cut(s)	742, 976
ApoI	RAATTY	8 cut(s)	96, 127, 359, 517, 1183, 1310, 1558, 2015
ArsI	GACNNNNNNTTYG	2 cut(s)	908, 940
AsiGI	ACCGGT	1 cut(s)	692
Asp700I	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
AspS9I	GGNCC	5 cut(s)	173, 251, 695, 880, 1079
AsuHPI	GGTGA	6 cut(s)	751, 1031, 1488, 1691, 1888, 1952
AvaI	CYCGRG	1 cut(s)	1510
AvaII	GGWCC	3 cut(s)	251, 695, 880
BaeGI	GKGCMC	1 cut(s)	988
BalI	TGGCCA	1 cut(s)	148
BanII	GRGCYC	1 cut(s)	357
Bbv12I	GWGCWC	2 cut(s)	357, 2137
BbvI	GCAGC	2 cut(s)	729, 963
BceAI	ACGGC	2 cut(s)	562, 1896
BcgI	CGANNNNNNTGC	2 cut(s)	702, 736
BciT130I	CCWGG	4 cut(s)	84, 884, 972, 1209
BcoDI	GTCTC	5 cut(s)	220, 585, 1701, 2130, 2191
BfaI	CTAG	8 cut(s)	486, 1137, 1392, 1704, 1749, 1794, 1907, 1946
BglII	AGATCT	1 cut(s)	787
BisI	GCNGC	2 cut(s)	743, 977
BlpI	GCTNAGC	1 cut(s)	287
BlsI	GCNGC	2 cut(s)	744, 978
Bme1390I	CCNGG	4 cut(s)	84, 884, 972, 1209
Bme18I	GGWCC	3 cut(s)	251, 695, 880
BmeT110I	CYCGRG	1 cut(s)	1510
BmgT120I	GGNCC	5 cut(s)	173, 251, 695, 880, 1079
BmiI	GGNNCC	4 cut(s)	73, 1080, 1680, 1844
BmrFI	CCNGG	4 cut(s)	84, 884, 972, 1209
BmrI	ACTGGG	1 cut(s)	1791
BmsI	GCATC	3 cut(s)	722, 1055, 2155
BmuI	ACTGGG	1 cut(s)	1791
BpmI	CTGGAG	1 cut(s)	34
Bpu10I	CCTNAGC	1 cut(s)	419
Bpu1102I	GCTNAGC	1 cut(s)	287
BpuEI	CTTGAG	3 cut(s)	785, 1282, 2112
Bsa29I	ATCGAT	1 cut(s)	2278
BsaI	GGTCTC	1 cut(s)	1701
BsaJI	CCNNGG	9 cut(s)	57, 82, 295, 883, 971, 1082, 1113, 1912, 2043
BsaWI	WCCGGW	3 cut(s)	692, 761, 926
BsaXI	ACNNNNNCTCC	2 cut(s)	2188, 2218
Bsc4I	CCNNNNNNNGG	4 cut(s)	155, 901, 1076, 1088
Bse118I	RCCGGY	2 cut(s)	692, 894
Bse1I	ACTGG	6 cut(s)	475, 622, 858, 1458, 1765, 1786
Bse3DI	GCAATG	3 cut(s)	208, 572, 986
BseBI	CCWGG	4 cut(s)	84, 884, 972, 1209
BseCI	ATCGAT	1 cut(s)	2278
BseDI	CCNNGG	9 cut(s)	57, 82, 295, 883, 971, 1082, 1113, 1912, 2043
BseGI	GGATG	6 cut(s)	479, 1549, 1843, 2017, 2221, 2310
BseLI	CCNNNNNNNGG	4 cut(s)	155, 901, 1076, 1088
BseMI	GCAATG	3 cut(s)	208, 572, 986
BseMII	CTCAG	7 cut(s)	275, 301, 600, 1041, 1121, 1239, 2215
BseNI	ACTGG	6 cut(s)	475, 622, 858, 1458, 1765, 1786
BseRI	GAGGAG	2 cut(s)	37, 1371
BseSI	GKGCMC	1 cut(s)	988
BseXI	GCAGC	2 cut(s)	729, 963
BsgI	GTGCAG	1 cut(s)	674
BshFI	GGCC	6 cut(s)	148, 174, 187, 417, 1081, 1911
BshTI	ACCGGT	1 cut(s)	692
BshVI	ATCGAT	1 cut(s)	2278
BsiHKAI	GWGCWC	2 cut(s)	357, 2137
BsiHKCI	CYCGRG	1 cut(s)	1510
BsiSI	CCGG	6 cut(s)	557, 629, 693, 762, 895, 927
BslFI	GGGAC	3 cut(s)	221, 255, 1969
BslI	CCNNNNNNNGG	4 cut(s)	155, 901, 1076, 1088
BsmAI	GTCTC	5 cut(s)	220, 585, 1701, 2130, 2191
BsmBI	CGTCTC	1 cut(s)	585
BsmFI	GGGAC	3 cut(s)	221, 255, 1969
BsmI	GAATGC	1 cut(s)	466
BsnI	GGCC	6 cut(s)	148, 174, 187, 417, 1081, 1911
Bso31I	GGTCTC	1 cut(s)	1701
BsoBI	CYCGRG	1 cut(s)	1510
Bsp1286I	GDGCHC	3 cut(s)	357, 988, 2137
Bsp143I	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
Bsp1720I	GCTNAGC	1 cut(s)	287
Bsp19I	CCATGG	1 cut(s)	57
BspACI	CCGC	5 cut(s)	845, 857, 1281, 1917, 2254
BspANI	GGCC	6 cut(s)	148, 174, 187, 417, 1081, 1911
BspCNI	CTCAG	7 cut(s)	274, 300, 599, 1042, 1120, 1240, 2214
BspDI	ATCGAT	1 cut(s)	2278
BspLI	GGNNCC	4 cut(s)	73, 1080, 1680, 1844
BspPI	GGATC	4 cut(s)	453, 556, 962, 1712
BspQI	GCTCTTC	2 cut(s)	1626, 2156
BspTNI	GGTCTC	1 cut(s)	1701
BsrDI	GCAATG	3 cut(s)	208, 572, 986
BsrFI	RCCGGY	2 cut(s)	692, 894
BsrI	ACTGG	6 cut(s)	475, 622, 858, 1458, 1765, 1786
BssAI	RCCGGY	2 cut(s)	692, 894
BssECI	CCNNGG	9 cut(s)	57, 82, 295, 883, 971, 1082, 1113, 1912, 2043
BssMI	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
BssT1I	CCWWGG	5 cut(s)	57, 295, 1082, 1113, 2043
Bst2UI	CCWGG	4 cut(s)	84, 884, 972, 1209
Bst4CI	ACNGT	5 cut(s)	1322, 1372, 1436, 1579, 1930
Bst6I	CTCTTC	2 cut(s)	1626, 2156
BstC8I	GCNNGC	3 cut(s)	1145, 2144, 2254
BstDSI	CCRYGG	2 cut(s)	57, 1912
BstEII	GGTNACC	1 cut(s)	1876
BstF5I	GGATG	6 cut(s)	479, 1549, 1843, 2017, 2221, 2310
BstKTI	GATC	7 cut(s)	448, 551, 790, 970, 1720, 2278, 2282
BstMAI	GTCTC	5 cut(s)	220, 585, 1701, 2130, 2191
BstMBI	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
BstMWI	GCNNNNNNNGC	4 cut(s)	71, 283, 297, 1598
BstNI	CCWGG	4 cut(s)	84, 884, 972, 1209
BstPI	GGTNACC	1 cut(s)	1876
BstSCI	CCNGG	4 cut(s)	82, 882, 970, 1207
BstSLI	GKGCMC	1 cut(s)	988
BstV1I	GCAGC	2 cut(s)	729, 963
BstX2I	RGATCY	1 cut(s)	787
BstXI	CCANNNNNNTGG	1 cut(s)	833
BstYI	RGATCY	1 cut(s)	787
Bsu15I	ATCGAT	1 cut(s)	2278
BsuRI	GGCC	6 cut(s)	148, 174, 187, 417, 1081, 1911
BsuTUI	ATCGAT	1 cut(s)	2278
BtgI	CCRYGG	2 cut(s)	57, 1912
BtgZI	GCGATG	1 cut(s)	2243
BtsCI	GGATG	6 cut(s)	479, 1549, 1843, 2017, 2221, 2310
BtsI	GCAGTG	1 cut(s)	1813
BtsIMutI	CAGTG	4 cut(s)	851, 1465, 1758, 1813
Cac8I	GCNNGC	3 cut(s)	1145, 2144, 2254
CaiI	CAGNNNCTG	1 cut(s)	853
Cfr10I	RCCGGY	2 cut(s)	692, 894
Cfr13I	GGNCC	5 cut(s)	173, 251, 695, 880, 1079
ClaI	ATCGAT	1 cut(s)	2278
CseI	GACGC	2 cut(s)	453, 1149
Csp6I	GTAC	4 cut(s)	196, 906, 1155, 2236
CspAI	ACCGGT	1 cut(s)	692
CspCI	CAANNNNNGTGG	2 cut(s)	1204, 1239
CviAII	CATG	6 cut(s)	58, 191, 746, 862, 1316, 2317
CviQI	GTAC	4 cut(s)	196, 906, 1155, 2236
DpnI	GATC	7 cut(s)	447, 550, 789, 969, 1719, 2277, 2281
DpnII	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
DrdI	GACNNNNNNGTC	1 cut(s)	249
DseDI	GACNNNNNNGTC	1 cut(s)	249
EaeI	YGGCCR	1 cut(s)	146
Eam1104I	CTCTTC	2 cut(s)	1626, 2156
EarI	CTCTTC	2 cut(s)	1626, 2156
EciI	GGCGGA	2 cut(s)	872, 1932
Ecl136II	GAGCTC	1 cut(s)	355
Eco130I	CCWWGG	5 cut(s)	57, 295, 1082, 1113, 2043
Eco24I	GRGCYC	1 cut(s)	357
Eco31I	GGTCTC	1 cut(s)	1701
Eco47I	GGWCC	3 cut(s)	251, 695, 880
Eco53kI	GAGCTC	1 cut(s)	355
Eco57I	CTGAAG	3 cut(s)	1346, 1786, 2195
Eco88I	CYCGRG	1 cut(s)	1510
Eco91I	GGTNACC	1 cut(s)	1876
EcoICRI	GAGCTC	1 cut(s)	355
EcoO109I	RGGNCCY	2 cut(s)	251, 1079
EcoO65I	GGTNACC	1 cut(s)	1876
EcoRI	GAATTC	2 cut(s)	127, 359
EcoRII	CCWGG	4 cut(s)	82, 882, 970, 1207
EcoT14I	CCWWGG	5 cut(s)	57, 295, 1082, 1113, 2043
EcoT22I	ATGCAT	1 cut(s)	35
EcoT38I	GRGCYC	1 cut(s)	357
ErhI	CCWWGG	5 cut(s)	57, 295, 1082, 1113, 2043
Esp3I	CGTCTC	1 cut(s)	585
FaeI	CATG	6 cut(s)	61, 194, 749, 865, 1319, 2320
FalI	AAGNNNNNCTT	4 cut(s)	151, 183, 2121, 2153
FaqI	GGGAC	3 cut(s)	221, 255, 1969
FatI	CATG	6 cut(s)	57, 190, 745, 861, 1315, 2316
FauI	CCCGC	1 cut(s)	2261
FauNDI	CATATG	1 cut(s)	1827
FblI	GTMKAC	2 cut(s)	467, 2112
Fnu4HI	GCNGC	2 cut(s)	743, 977
FokI	GGATG	6 cut(s)	486, 1556, 1850, 2024, 2228, 2317
FriOI	GRGCYC	1 cut(s)	357
Fsp4HI	GCNGC	2 cut(s)	743, 977
FspBI	CTAG	8 cut(s)	486, 1137, 1392, 1704, 1749, 1794, 1907, 1946
GluI	GCNGC	2 cut(s)	743, 977
GsuI	CTGGAG	1 cut(s)	34
HaeIII	GGCC	6 cut(s)	148, 174, 187, 417, 1081, 1911
HapII	CCGG	6 cut(s)	557, 629, 693, 762, 895, 927
HgaI	GACGC	2 cut(s)	453, 1149
Hin1II	CATG	6 cut(s)	61, 194, 749, 865, 1319, 2320
HincII	GTYRAC	2 cut(s)	729, 1773
HindII	GTYRAC	2 cut(s)	729, 1773
HindIII	AAGCTT	2 cut(s)	866, 1765
HpaII	CCGG	6 cut(s)	557, 629, 693, 762, 895, 927
HphI	GGTGA	6 cut(s)	751, 1031, 1488, 1691, 1888, 1952
Hpy166II	GTNNAC	9 cut(s)	196, 468, 729, 1418, 1490, 1773, 1933, 1943, 2113
Hpy188III	TCNNGA	4 cut(s)	13, 677, 1392, 1721
Hpy8I	GTNNAC	9 cut(s)	196, 468, 729, 1418, 1490, 1773, 1933, 1943, 2113
Hpy99I	CGWCG	3 cut(s)	727, 1343, 2090
HpyAV	CCTTC	4 cut(s)	12, 1176, 1321, 2084
HpyCH4III	ACNGT	5 cut(s)	1322, 1372, 1436, 1579, 1930
HpyF10VI	GCNNNNNNNGC	4 cut(s)	71, 283, 297, 1598
Hsp92II	CATG	6 cut(s)	61, 194, 749, 865, 1319, 2320
Kzo9I	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
LguI	GCTCTTC	2 cut(s)	1626, 2156
LmnI	GCTCC	1 cut(s)	71
Lsp1109I	GCAGC	2 cut(s)	729, 963
LweI	GCATC	3 cut(s)	722, 1055, 2155
MaeI	CTAG	8 cut(s)	486, 1137, 1392, 1704, 1749, 1794, 1907, 1946
MaeIII	GTNAC	5 cut(s)	161, 849, 1796, 1876, 2078
MalI	GATC	7 cut(s)	447, 550, 789, 969, 1719, 2277, 2281
MboI	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
MboII	GAAGA	5 cut(s)	786, 1613, 1961, 2173, 2279
MfeI	CAATTG	1 cut(s)	509
MflI	RGATCY	1 cut(s)	787
MhlI	GDGCHC	3 cut(s)	357, 988, 2137
MlsI	TGGCCA	1 cut(s)	148
MluNI	TGGCCA	1 cut(s)	148
MlyI	GAGTC	5 cut(s)	222, 237, 653, 1522, 2193
MmeI	TCCRAC	2 cut(s)	102, 2193
Mox20I	TGGCCA	1 cut(s)	148
Mph1103I	ATGCAT	1 cut(s)	35
MroXI	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
MscI	TGGCCA	1 cut(s)	148
MseI	TTAA	6 cut(s)	606, 614, 921, 1037, 1181, 1971
MslI	CAYNNNNRTG	1 cut(s)	1051
Msp20I	TGGCCA	1 cut(s)	148
MspI	CCGG	6 cut(s)	557, 629, 693, 762, 895, 927
MspR9I	CCNGG	4 cut(s)	84, 884, 972, 1209
MunI	CAATTG	1 cut(s)	509
Mva1269I	GAATGC	1 cut(s)	466
MvaI	CCWGG	4 cut(s)	84, 884, 972, 1209
MwoI	GCNNNNNNNGC	4 cut(s)	71, 283, 297, 1598
NcoI	CCATGG	1 cut(s)	57
NdeI	CATATG	1 cut(s)	1827
NdeII	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
NlaIII	CATG	6 cut(s)	61, 194, 749, 865, 1319, 2320
NlaIV	GGNNCC	4 cut(s)	73, 1080, 1680, 1844
NmuCI	GTSAC	3 cut(s)	161, 849, 1876
NsiI	ATGCAT	1 cut(s)	35
PaeR7I	CTCGAG	1 cut(s)	1510
PciSI	GCTCTTC	2 cut(s)	1626, 2156
PctI	GAATGC	1 cut(s)	466
PdmI	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
PfeI	GAWTC	5 cut(s)	223, 625, 841, 1162, 1596
PflFI	GACNNNGTC	1 cut(s)	212
PflMI	CCANNNNNTGG	1 cut(s)	155
PfoI	TCCNGGA	1 cut(s)	1207
PinAI	ACCGGT	1 cut(s)	692
PkrI	GCNGC	2 cut(s)	744, 978
PleI	GAGTC	5 cut(s)	221, 237, 652, 1521, 2193
PpsI	GAGTC	5 cut(s)	221, 237, 652, 1521, 2193
PpuMI	RGGWCCY	1 cut(s)	251
PsiI	TTATAA	1 cut(s)	942
Psp124BI	GAGCTC	1 cut(s)	357
Psp5II	RGGWCCY	1 cut(s)	251
Psp6I	CCWGG	4 cut(s)	82, 882, 970, 1207
PspEI	GGTNACC	1 cut(s)	1876
PspGI	CCWGG	4 cut(s)	82, 882, 970, 1207
PspN4I	GGNNCC	4 cut(s)	73, 1080, 1680, 1844
PspPI	GGNCC	5 cut(s)	173, 251, 695, 880, 1079
PspPPI	RGGWCCY	1 cut(s)	251
PspXI	VCTCGAGB	1 cut(s)	1510
PstNI	CAGNNNCTG	1 cut(s)	853
PsuI	RGATCY	1 cut(s)	787
PsyI	GACNNNGTC	1 cut(s)	212
RsaI	GTAC	4 cut(s)	197, 907, 1156, 2237
RsaNI	GTAC	4 cut(s)	196, 906, 1155, 2236
RseI	CAYNNNNRTG	1 cut(s)	1051
SacI	GAGCTC	1 cut(s)	357
SapI	GCTCTTC	2 cut(s)	1626, 2156
SaqAI	TTAA	6 cut(s)	606, 614, 921, 1037, 1181, 1971
SatI	GCNGC	2 cut(s)	743, 977
Sau3AI	GATC	7 cut(s)	445, 548, 787, 967, 1717, 2275, 2279
Sau96I	GGNCC	5 cut(s)	173, 251, 695, 880, 1079
SchI	GAGTC	5 cut(s)	222, 237, 653, 1522, 2193
ScrFI	CCNGG	4 cut(s)	84, 884, 972, 1209
SduI	GDGCHC	3 cut(s)	357, 988, 2137
SfaNI	GCATC	3 cut(s)	722, 1055, 2155
Sfr274I	CTCGAG	1 cut(s)	1510
SinI	GGWCC	3 cut(s)	251, 695, 880
SlaI	CTCGAG	1 cut(s)	1510
SmiMI	CAYNNNNRTG	1 cut(s)	1051
SmlI	CTYRAG	4 cut(s)	800, 1261, 1510, 2127
SmoI	CTYRAG	4 cut(s)	800, 1261, 1510, 2127
SsiI	CCGC	5 cut(s)	845, 857, 1281, 1917, 2254
SspI	AATATT	1 cut(s)	2295
SspMI	CTAG	8 cut(s)	486, 1137, 1392, 1704, 1749, 1794, 1907, 1946
SstI	GAGCTC	1 cut(s)	357
StyD4I	CCNGG	4 cut(s)	82, 882, 970, 1207
StyI	CCWWGG	5 cut(s)	57, 295, 1082, 1113, 2043
TaaI	ACNGT	5 cut(s)	1322, 1372, 1436, 1579, 1930
TaqI	TCGA	9 cut(s)	357, 387, 402, 1293, 1338, 1511, 2056, 2088, 2278
TatI	WGTACW	1 cut(s)	195
TfiI	GAWTC	5 cut(s)	223, 625, 841, 1162, 1596
Tru1I	TTAA	6 cut(s)	606, 614, 921, 1037, 1181, 1971
Tru9I	TTAA	6 cut(s)	606, 614, 921, 1037, 1181, 1971
TscAI	CASTG	4 cut(s)	858, 1465, 1765, 1813
TseFI	GTSAC	3 cut(s)	161, 849, 1876
TseI	GCWGC	2 cut(s)	742, 976
Tsp45I	GTSAC	3 cut(s)	161, 849, 1876
TspDTI	ATGAA	6 cut(s)	878, 2028, 2112, 2144, 2254, 2305
TspGWI	ACGGA	5 cut(s)	444, 1059, 1111, 1356, 2074
TspRI	CASTG	4 cut(s)	858, 1465, 1765, 1813
Tth111I	GACNNNGTC	1 cut(s)	212
Van91I	CCANNNNNTGG	1 cut(s)	155
VpaK11BI	GGWCC	3 cut(s)	251, 695, 880
XapI	RAATTY	8 cut(s)	96, 127, 359, 517, 1183, 1310, 1558, 2015
XbaI	TCTAGA	1 cut(s)	1391
XhoI	CTCGAG	1 cut(s)	1510
XmiI	GTMKAC	2 cut(s)	467, 2112
XmnI	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
XspI	CTAG	8 cut(s)	486, 1137, 1392, 1704, 1749, 1794, 1907, 1946
Zsp2I	ATGCAT	1 cut(s)	35

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.