Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0003268

Physical Location & Seq

Genomic Coordinates Reverse (-)

13881737 .. 13886022

Gene Structure 4286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g20300.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2838 bp

ATGGCCGGCCCTAACCTCATAACCTCTCTCCTCCTCCTCCTCTCCCTCATCTCAGTGGCCTTCACCGCCACAGACCCAAATGACCTGGCCATTCTCAACCAGTTCCGAAAAAACATGGAGAATTCCGACCTCCTCAACTGGCCTGAAACCGGCGACGACCCCTGTGGCCCTCCAAAGTGGGACCACGTCTTCTGCTCCGGCGACAGGGTCTCCCAGATTCAGGTCCAAAACCTAGGCCTGAAGGGTCCTCTCCCCCAAAACCTCAACCAGCTCTCTGAGCTCTTCAACATCGGCCTCCAACGCAACCAGTTCTCCGGTCCCTTGCCCACCCTCAAAGGCTTGTCCAAACTCAAGTACGCTTTTCTTGATTACAACAACTTCACTTCAATCCCCGGCGACTTCTTCGTCGGCCTTGACGCCCTGGAGGTTCTGGCATTGGACGGCCTTGAGTTGAATGCCTCTACTGGCTGGACATTGCCGATAGATTTATCCAATTCGGTGCAGCTGCAGAATCTGACGTGTCTGAGCTGCAATTTGGTGGGGCCCTTGCCCGAGTTTCTCGGGAATTTGACTTCCCTGACGGTGCTGGAGCTTTCCGGGAATGGATTGAGTGGGGAGATTCCGGCGAGTTTTAAGGGGCTGAACTTGCAGAGCTTGAGGTTGAACAACCCGAAAGGCGCCGGGTTGAGCGGCGGTATTGATGTGATCGCTACAATGGTGCAGCTCAACTCGGCGTGGCTTCATGGGAATCAGTTCACAGGTAGTATTCCGGAGAGTATTGGTGATTTGGTTTCTTTGAAGGATCTTAATTTGAATGGGAATAAACTTAGTGGTGTGATTCCTGATGGTTTGGCTAATTTGGAACTTGATACATTGAATTTGAATAATAACCATTTTATGGGTCCTATACCAACGTTTAAAGCAAAGAATGTGAGTTATGAGTCTAATGCATTTTGCCAAGACACTCCGGGGGTGCCTTGTGCGCCGGAGGTCATGGCGCTTATTGAGTTTCTTGGGGGGTTGGATTACCCTACAACGCTTGCTGATGATTGGTCTGGTAATGATCCTTGCAAAGGGTCATGGTTGGGAGTGAGTTGTAGCAACAATGGGAAGGTGTCTGTTATAAATCTACCCAACTTTAAGCTCAATGGTACATTGAGTCCTTCAGTTGCCAAGTTAGATTCTCTTACTCAGATTAAGCTTCAGGGTAACAATTTGAAGGGTCCAATTCCTGCAAACTGGACTAGCTTGAAAGCCCTGACGGTGCTGGATCTAACTGGGAATGATATTACCCCTCCATTGCCGAAATTTGTCAACACGGTTAAAGTGGTCATTGATGGGAATCCTTTGTTTCATGGTAATCCATCTGAGCAAGGCCCGGCACCAGAGAGTAATTCTACATCAACCAACCCATCTTCCCCGACAAACACAAGTTCTAATGGGGATTCAAAAGGATCAAAAGGGCCCAACATAGTTTCAATTGTTGCTCCTGTCACAAGTGTTGCTGTTGTTGCTTTGCTTGTAATTCCTTTGTCCATCTACTATTGTAAGAAGAGAAGAACTGGCTTCCAGGCTCCAAGTTCCCTTGTAGTTCACCCAAGAGATCCATCTGATTCAGATAATACAGTTAAGATTGTTGTTGCCAGTAATACCAATGGAAGCACTTCTACCCTGACCAGGAGTGGTTCTGCAAGCAGAAACAGCAGTGGGATAGGCGAGTCTCATGTCATTGAAGCTGGAAATCTTGTCATATCAGTTCAAGTTCTTCGGAATGTGACAAAGAATTTTGCCCCCGAAAATGAGCTTGGTCGTGGTGGTTTTGGAGTTGTATATAAGGGAGAGTTGGATGATGGGACAAAAATAGCAGTGAAAAGAATGGAGGCAGGTGTGATCAGCAACAAAGCCTTGGATGAATTCCAGTCTGAAATTGCAGTTCTTTCAAAGGTCCGACACCGTCATCTGGTATCGCTGTTGGGTTATTCTGTTGCAGGAAATGAGAGAATGCTTGTGTATGAATATATGCCTCAAGGGGCTCTAAGCAGGCATCTTTTTCATTGGAAAACCTTTAAACTAGAACCTCTCTCTTGGACGAGGAGGCTAAACATTGCCTTGGACGTCGCTAGGGGGCTCGAGTATCTTCATAATTTGGCACAGCAAAGCTTCATACACAGAGACCTCAAGTCATCAAACATCTTACTTGGTGATGATTTTAAAGCAAAAATATCTGATTTTGGATTGGTGAAACTGGCTCCGAATGGTGAAAGATCTGTTGTGACCAAGCTTGCTGGAACTTTTGGTTACTTAGCACCAGAGTATGCTGTGACGGGAAAAATTACAACCAAAGTAGATGTGTTCAGTTTCGGGGTTGTGTTAATGGAGCTGTTAACTGGAATGATGGCGCTGGATGATGATAGGCCTGAAGAAAAGCAGTACTTGGCTGCATGGTTCTGGCACATCAAATCAAATAAGGAGAAACTGTTGGCAGCCATTGACCCAACCCTTGACATAAAAGACATGAAAGAAGAAACATTTGAGAGCATCGCCACAATTGCGGAACTAGCTGGTCACTGCACTGCAAGAGAACCCAGCCAAAGACCAGATATGGGGCATGCTGTGAATGTATTGTCCCCGCTTGTTGAAAAATGGAAACCATTTAATGATGAGGATGATGAATATTCTGGCATAGACTACAGCCTCCCGCTTAACCAGATGGTGAAAGGTTGGCAGGAAGCAGAAGGAAAGGACTCTGGTTACATAGACCTAGAAGATAGCAAGGGCAGTATACCAGCACGGCCAACTGGGTTTGCGGACTCTTTTACTTCAGCTGATGGTCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

946

Amino Acids

101.96

Weight (kDa)

5.29

Isoelectric Point (pI)

28.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	329 - 367	4e-06	Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr	PF07714	599 - 873	3.4e-46	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	599 - 872	4.2e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003268)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g26630 FvH4_3g26630 FvH4_6g20300
malus_domestica	MD03G1154600.v1.1 MD12G1040200.v1.1 MD14G1038700.v1.1
prunus_persica	Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.7G094700_v2.0.a1
pyrus_communis	pycom03g10840 pycom12g03440 pycom14g03340
rosa_chinensis	RchiOBHm_Chr3g0475921 RchiOBHm_Chr5g0048201
rosa_laevigata	RLG00000023801 RLG00000034502
rosa_multiflora	Rmu_sc0001408.1_g000015 Rmu_sc0009168.1_g000004
rosa_roxburghii	Rroxscaffold_1G00033720 Rroxscaffold_6G00405730
rosa_rugosa	Rorug03G0151900 Rorug05G0237000 Rorug05G0237100
rosa_samantha	Rh3AG202700 Rh3BG232600 Rh3CG228000 Rh3DG228200 Rh5AG316200 Rh5BG326800 Rh5CG353000 Rh5DG337800
rosa_wichuraiana	Rw3G018440 Rw5G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1124
AarI	CACCTGC	1 cut(s)	1874
AasI	GACNNNNNNGTC	1 cut(s)	404
AatII	GACGTC	1 cut(s)	2118
Acc36I	ACCTGC	1 cut(s)	1874
AccB1I	GGYRCC	3 cut(s)	677, 973, 1381
AccB7I	CCANNNNNTGG	1 cut(s)	898
AccBSI	CCGCTC	1 cut(s)	690
AccI	GTMKAC	1 cut(s)	2782
AccIII	TCCGGA	1 cut(s)	769
AciI	CCGC	7 cut(s)	66, 690, 693, 2552, 2630, 2699, 2807
AclI	AACGTT	1 cut(s)	914
AclWI	GGATC	5 cut(s)	810, 1058, 1278, 1462, 1598
AcoI	YGGCCR	3 cut(s)	3, 87, 2792
AcsI	RAATTY	6 cut(s)	121, 565, 877, 1307, 1783, 1913
AcuI	CTGAAG	5 cut(s)	260, 1149, 1187, 2439, 2805
AcyI	GRCGYC	3 cut(s)	417, 678, 2115
AfaI	GTAC	3 cut(s)	356, 1153, 2432
AfiI	CCNNNNNNNGG	8 cut(s)	149, 204, 220, 898, 1073, 1677, 1960, 2603
AflIII	ACRYGT	1 cut(s)	518
AhdI	GACNNNNNGTC	1 cut(s)	2179
AjiI	CACGTC	2 cut(s)	187, 519
AjnI	CCWGG	4 cut(s)	84, 420, 1569, 1676
AjuI	GAANNNNNNNTTGG	2 cut(s)	1788, 1820
Alw21I	GWGCWC	1 cut(s)	282
Alw26I	GTCTC	3 cut(s)	214, 1725, 2166
AlwI	GGATC	5 cut(s)	810, 1058, 1278, 1462, 1598
AlwNI	CAGNNNCTG	1 cut(s)	514
Ama87I	CYCGRG	3 cut(s)	551, 560, 2129
Aor13HI	TCCGGA	1 cut(s)	769
ApaI	GGGCCC	2 cut(s)	546, 1467
ApeKI	GCWGC	6 cut(s)	502, 505, 528, 721, 2438, 2483
ApoI	RAATTY	6 cut(s)	121, 565, 877, 1307, 1783, 1913
Asp700I	GAANNNNTTC	2 cut(s)	752, 1663
AspA2I	CCTAGG	1 cut(s)	232
AspLEI	GCGC	4 cut(s)	680, 985, 1000, 2401
AsuC2I	CCSGG	5 cut(s)	393, 598, 682, 969, 1379
AsuHPI	GGTGA	7 cut(s)	55, 794, 1586, 2213, 2251, 2270, 2725
AvaI	CYCGRG	3 cut(s)	551, 560, 2129
AvaII	GGWCC	7 cut(s)	181, 223, 245, 317, 902, 1223, 1945
AvrII	CCTAGG	1 cut(s)	232
BaeGI	GKGCMC	2 cut(s)	546, 1467
BalI	TGGCCA	1 cut(s)	89
BanI	GGYRCC	3 cut(s)	677, 973, 1381
BanII	GRGCYC	5 cut(s)	282, 546, 1467, 2035, 2130
BarI	GAAGNNNNNNTAC	2 cut(s)	1651, 1683
BbsI	GAAGAC	1 cut(s)	181
Bbv12I	GWGCWC	1 cut(s)	282
BbvI	GCAGC	6 cut(s)	492, 514, 515, 733, 2425, 2495
BceAI	ACGGC	2 cut(s)	457, 2807
BciT130I	CCWGG	4 cut(s)	86, 422, 1571, 1678
BclI	TGATCA	1 cut(s)	1890
BcnI	CCSGG	5 cut(s)	393, 598, 682, 969, 1379
BcoDI	GTCTC	3 cut(s)	214, 1725, 2166
BfaI	CTAG	6 cut(s)	233, 1245, 2072, 2121, 2558, 2762
BfmI	CTRYAG	2 cut(s)	506, 2689
BfoI	RGCGCY	3 cut(s)	681, 1001, 2402
BfuAI	ACCTGC	1 cut(s)	1874
BglII	AGATCT	1 cut(s)	2264
BisI	GCNGC	7 cut(s)	503, 506, 529, 691, 722, 2439, 2484
BlnI	CCTAGG	1 cut(s)	232
BlsI	GCNGC	7 cut(s)	504, 507, 530, 692, 723, 2440, 2485
BmcAI	AGTACT	1 cut(s)	2432
Bme1390I	CCNGG	9 cut(s)	86, 393, 422, 598, 682, 969, 1379, 1571, 1678
Bme18I	GGWCC	7 cut(s)	181, 223, 245, 317, 902, 1223, 1945
BmeRI	GACNNNNNGTC	1 cut(s)	2179
BmeT110I	CYCGRG	3 cut(s)	551, 560, 2129
BmgBI	CACGTC	2 cut(s)	187, 519
BmrFI	CCNGG	9 cut(s)	86, 393, 422, 598, 682, 969, 1379, 1571, 1678
BmrI	ACTGGG	2 cut(s)	1287, 2808
BmsI	GCATC	2 cut(s)	2053, 2547
BmuI	ACTGGG	2 cut(s)	1287, 2808
BpiI	GAAGAC	1 cut(s)	181
BpmI	CTGGAG	2 cut(s)	443, 608
BpuEI	CTTGAG	5 cut(s)	335, 467, 676, 2010, 2162
BpuMI	CCSGG	5 cut(s)	393, 598, 682, 969, 1379
BsaBI	GATNNNNATC	1 cut(s)	1341
BsaHI	GRCGYC	3 cut(s)	417, 678, 2115
BsaI	GGTCTC	2 cut(s)	214, 2166
BsaJI	CCNNGG	6 cut(s)	232, 391, 420, 968, 1905, 2109
BsaWI	WCCGGW	2 cut(s)	314, 769
BsaXI	ACNNNNNCTCC	6 cut(s)	194, 224, 296, 326, 2462, 2492
Bsc4I	CCNNNNNNNGG	8 cut(s)	149, 204, 220, 898, 1073, 1677, 1960, 2603
Bse118I	RCCGGY	2 cut(s)	5, 149
Bse3DI	GCAATG	3 cut(s)	473, 1298, 2103
Bse8I	GATNNNNATC	1 cut(s)	1341
BseAI	TCCGGA	1 cut(s)	769
BseBI	CCWGG	4 cut(s)	86, 422, 1571, 1678
BseDI	CCNNGG	6 cut(s)	232, 391, 420, 968, 1905, 2109
BseGI	GGATG	4 cut(s)	1852, 1915, 2410, 2671
BseJI	GATNNNNATC	1 cut(s)	1341
BseLI	CCNNNNNNNGG	8 cut(s)	149, 204, 220, 898, 1073, 1677, 1960, 2603
BseMI	GCAATG	3 cut(s)	473, 1298, 2103
BseMII	CTCAG	5 cut(s)	66, 267, 515, 1205, 1359
BseRI	GAGGAG	6 cut(s)	20, 23, 26, 29, 122, 2107
BseSI	GKGCMC	2 cut(s)	546, 1467
BseXI	GCAGC	6 cut(s)	492, 514, 515, 733, 2425, 2495
BseYI	CCCAGC	1 cut(s)	2585
BsgI	GTGCAG	3 cut(s)	521, 740, 2554
BshNI	GGYRCC	3 cut(s)	677, 973, 1381
BsiHKAI	GWGCWC	1 cut(s)	282
BsiHKCI	CYCGRG	3 cut(s)	551, 560, 2129
BslFI	GGGAC	4 cut(s)	194, 303, 1867, 2611
BslI	CCNNNNNNNGG	8 cut(s)	149, 204, 220, 898, 1073, 1677, 1960, 2603
BsmAI	GTCTC	3 cut(s)	214, 1725, 2166
BsmFI	GGGAC	4 cut(s)	194, 303, 1867, 2611
BsmI	GAATGC	2 cut(s)	460, 2007
Bso31I	GGTCTC	2 cut(s)	214, 2166
BsoBI	CYCGRG	3 cut(s)	551, 560, 2129
Bsp120I	GGGCCC	2 cut(s)	542, 1463
Bsp1286I	GDGCHC	5 cut(s)	282, 546, 1467, 2035, 2130
Bsp13I	TCCGGA	1 cut(s)	769
Bsp143I	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
BspACI	CCGC	7 cut(s)	66, 690, 693, 2552, 2630, 2699, 2807
BspCNI	CTCAG	5 cut(s)	65, 268, 516, 1204, 1360
BspEI	TCCGGA	1 cut(s)	769
BspMAI	CTGCAG	1 cut(s)	510
BspMI	ACCTGC	1 cut(s)	1874
BspPI	GGATC	5 cut(s)	810, 1058, 1278, 1462, 1598
BspQI	GCTCTTC	1 cut(s)	287
BspT107I	GGYRCC	3 cut(s)	677, 973, 1381
BspTNI	GGTCTC	2 cut(s)	214, 2166
BsrBI	CCGCTC	1 cut(s)	690
BsrDI	GCAATG	3 cut(s)	473, 1298, 2103
BsrFI	RCCGGY	2 cut(s)	5, 149
BssAI	RCCGGY	2 cut(s)	5, 149
BssECI	CCNNGG	6 cut(s)	232, 391, 420, 968, 1905, 2109
BssMI	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
BssNAI	GTATAC	1 cut(s)	2783
BssNI	GRCGYC	3 cut(s)	417, 678, 2115
BssT1I	CCWWGG	3 cut(s)	232, 1905, 2109
Bst1107I	GTATAC	1 cut(s)	2783
Bst2UI	CCWGG	4 cut(s)	86, 422, 1571, 1678
Bst4CI	ACNGT	6 cut(s)	583, 1264, 1321, 1627, 1955, 2478
Bst6I	CTCTTC	2 cut(s)	287, 1547
BstACI	GRCGYC	3 cut(s)	417, 678, 2115
BstC8I	GCNNGC	6 cut(s)	7, 1041, 1693, 2042, 2283, 2610
BstDEI	CTNAG	8 cut(s)	52, 276, 524, 827, 1191, 1368, 2036, 2302
BstENI	CCTNNNNNAGG	1 cut(s)	1071
BstF5I	GGATG	4 cut(s)	1852, 1915, 2410, 2671
BstH2I	RGCGCY	3 cut(s)	681, 1001, 2402
BstHHI	GCGC	4 cut(s)	680, 985, 1000, 2401
BstKTI	GATC	8 cut(s)	708, 805, 1066, 1273, 1457, 1606, 1893, 2267
BstMAI	GTCTC	3 cut(s)	214, 1725, 2166
BstMBI	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
BstMWI	GCNNNNNNNGC	9 cut(s)	65, 277, 300, 646, 982, 1511, 1701, 2549, 2558
BstNI	CCWGG	4 cut(s)	86, 422, 1571, 1678
BstNSI	RCATGY	1 cut(s)	2612
BstSCI	CCNGG	9 cut(s)	84, 391, 420, 596, 680, 967, 1377, 1569, 1676
BstSFI	CTRYAG	2 cut(s)	506, 2689
BstSLI	GKGCMC	2 cut(s)	546, 1467
BstV1I	GCAGC	6 cut(s)	492, 514, 515, 733, 2425, 2495
BstV2I	GAAGAC	1 cut(s)	181
BstX2I	RGATCY	4 cut(s)	802, 1270, 1603, 2264
BstYI	RGATCY	4 cut(s)	802, 1270, 1603, 2264
BstZ17I	GTATAC	1 cut(s)	2783
BtgZI	GCGATG	1 cut(s)	2524
BtrI	CACGTC	2 cut(s)	187, 519
BtsCI	GGATG	4 cut(s)	1852, 1915, 2410, 2671
BtsI	GCAGTG	4 cut(s)	1711, 1872, 2566, 2571
BtsIMutI	CAGTG	5 cut(s)	60, 1711, 1872, 2566, 2571
BveI	ACCTGC	1 cut(s)	1874
Cac8I	GCNNGC	6 cut(s)	7, 1041, 1693, 2042, 2283, 2610
CaiI	CAGNNNCTG	1 cut(s)	514
CfoI	GCGC	4 cut(s)	680, 985, 1000, 2401
Cfr10I	RCCGGY	2 cut(s)	5, 149
CseI	GACGC	1 cut(s)	425
Csp6I	GTAC	3 cut(s)	355, 1152, 2431
CviAII	CATG	9 cut(s)	115, 743, 994, 1080, 1355, 1724, 2442, 2515, 2609
CviQI	GTAC	3 cut(s)	355, 1152, 2431
DdeI	CTNAG	8 cut(s)	52, 276, 524, 827, 1191, 1368, 2036, 2302
DinI	GGCGCC	1 cut(s)	679
DpnI	GATC	8 cut(s)	707, 804, 1065, 1272, 1456, 1605, 1892, 2266
DpnII	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
DraI	TTTAAA	3 cut(s)	919, 2068, 2212
DrdI	GACNNNNNNGTC	1 cut(s)	404
DriI	GACNNNNNGTC	1 cut(s)	2179
DseDI	GACNNNNNNGTC	1 cut(s)	404
EaeI	YGGCCR	3 cut(s)	3, 87, 2792
Eam1104I	CTCTTC	2 cut(s)	287, 1547
Eam1105I	GACNNNNNGTC	1 cut(s)	2179
EarI	CTCTTC	2 cut(s)	287, 1547
Ecl136II	GAGCTC	1 cut(s)	280
Eco130I	CCWWGG	3 cut(s)	232, 1905, 2109
Eco147I	AGGCCT	2 cut(s)	237, 2416
Eco24I	GRGCYC	5 cut(s)	282, 546, 1467, 2035, 2130
Eco31I	GGTCTC	2 cut(s)	214, 2166
Eco47I	GGWCC	7 cut(s)	181, 223, 245, 317, 902, 1223, 1945
Eco53kI	GAGCTC	1 cut(s)	280
Eco57I	CTGAAG	5 cut(s)	260, 1149, 1187, 2439, 2805
Eco88I	CYCGRG	3 cut(s)	551, 560, 2129
EcoICRI	GAGCTC	1 cut(s)	280
EcoNI	CCTNNNNNAGG	1 cut(s)	1071
EcoO109I	RGGNCCY	5 cut(s)	245, 542, 543, 902, 1463
EcoRI	GAATTC	2 cut(s)	121, 1913
EcoRII	CCWGG	4 cut(s)	84, 420, 1569, 1676
EcoT14I	CCWWGG	3 cut(s)	232, 1905, 2109
EcoT22I	ATGCAT	1 cut(s)	952
EcoT38I	GRGCYC	5 cut(s)	282, 546, 1467, 2035, 2130
EgeI	GGCGCC	1 cut(s)	679
EheI	GGCGCC	1 cut(s)	679
ErhI	CCWWGG	3 cut(s)	232, 1905, 2109
FaeI	CATG	9 cut(s)	118, 746, 997, 1083, 1358, 1727, 2445, 2518, 2612
FalI	AAGNNNNNCTT	2 cut(s)	2417, 2449
FaqI	GGGAC	4 cut(s)	194, 303, 1867, 2611
FatI	CATG	9 cut(s)	114, 742, 993, 1079, 1354, 1723, 2441, 2514, 2608
FauI	CCCGC	2 cut(s)	2637, 2706
FbaI	TGATCA	1 cut(s)	1890
FblI	GTMKAC	1 cut(s)	2782
Fnu4HI	GCNGC	7 cut(s)	503, 506, 529, 691, 722, 2439, 2484
FokI	GGATG	4 cut(s)	1859, 1922, 2417, 2678
FriOI	GRGCYC	5 cut(s)	282, 546, 1467, 2035, 2130
FseI	GGCCGGCC	1 cut(s)	9
Fsp4HI	GCNGC	7 cut(s)	503, 506, 529, 691, 722, 2439, 2484
FspBI	CTAG	6 cut(s)	233, 1245, 2072, 2121, 2558, 2762
GlaI	GCGC	4 cut(s)	679, 984, 999, 2400
GluI	GCNGC	7 cut(s)	503, 506, 529, 691, 722, 2439, 2484
GsaI	CCCAGC	1 cut(s)	2589
GsuI	CTGGAG	2 cut(s)	443, 608
HaeII	RGCGCY	3 cut(s)	681, 1001, 2402
HgaI	GACGC	1 cut(s)	425
HhaI	GCGC	4 cut(s)	680, 985, 1000, 2401
Hin1I	GRCGYC	3 cut(s)	417, 678, 2115
Hin1II	CATG	9 cut(s)	118, 746, 997, 1083, 1358, 1727, 2445, 2518, 2612
Hin6I	GCGC	4 cut(s)	678, 983, 998, 2399
HinP1I	GCGC	4 cut(s)	678, 983, 998, 2399
HincII	GTYRAC	2 cut(s)	1315, 2385
HindII	GTYRAC	2 cut(s)	1315, 2385
HindIII	AAGCTT	3 cut(s)	1199, 2158, 2279
HpaI	GTTAAC	1 cut(s)	2385
HphI	GGTGA	7 cut(s)	55, 794, 1586, 2213, 2251, 2270, 2725
Hpy166II	GTNNAC	5 cut(s)	756, 1315, 1594, 2385, 2783
Hpy188III	TCNNGA	4 cut(s)	365, 562, 770, 842
Hpy8I	GTNNAC	5 cut(s)	756, 1315, 1594, 2385, 2783
Hpy99I	CGWCG	3 cut(s)	158, 410, 2120
HpyAV	CCTTC	7 cut(s)	70, 235, 793, 1105, 1173, 1213, 2729
HpyCH4III	ACNGT	6 cut(s)	583, 1264, 1321, 1627, 1955, 2478
HpyCH4IV	ACGT	4 cut(s)	186, 518, 914, 2115
HpyF10VI	GCNNNNNNNGC	9 cut(s)	65, 277, 300, 646, 982, 1511, 1701, 2549, 2558
HpyF3I	CTNAG	8 cut(s)	52, 276, 524, 827, 1191, 1368, 2036, 2302
HpySE526I	ACGT	4 cut(s)	186, 518, 914, 2115
Hsp92I	GRCGYC	3 cut(s)	417, 678, 2115
Hsp92II	CATG	9 cut(s)	118, 746, 997, 1083, 1358, 1727, 2445, 2518, 2612
HspAI	GCGC	4 cut(s)	678, 983, 998, 2399
KasI	GGCGCC	1 cut(s)	677
Kpn2I	TCCGGA	1 cut(s)	769
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	1890
KspAI	GTTAAC	1 cut(s)	2385
Kzo9I	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
LguI	GCTCTTC	1 cut(s)	287
LmnI	GCTCC	6 cut(s)	200, 589, 1492, 1579, 2254, 2377
Lsp1109I	GCAGC	6 cut(s)	492, 514, 515, 733, 2425, 2495
LweI	GCATC	2 cut(s)	2053, 2547
MaeI	CTAG	6 cut(s)	233, 1245, 2072, 2121, 2558, 2762
MaeII	ACGT	4 cut(s)	186, 518, 914, 2115
MaeIII	GTNAC	8 cut(s)	1208, 1492, 1774, 2272, 2297, 2320, 2564, 2750
MalI	GATC	8 cut(s)	707, 804, 1065, 1272, 1456, 1605, 1892, 2266
MbiI	CCGCTC	1 cut(s)	690
MboI	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
MfeI	CAATTG	2 cut(s)	1479, 2547
MflI	RGATCY	4 cut(s)	802, 1270, 1603, 2264
MhlI	GDGCHC	5 cut(s)	282, 546, 1467, 2035, 2130
MlsI	TGGCCA	1 cut(s)	89
MluNI	TGGCCA	1 cut(s)	89
Mly113I	GGCGCC	1 cut(s)	678
MlyI	GAGTC	5 cut(s)	950, 1168, 1727, 2738, 2804
MmeI	TCCRAC	5 cut(s)	150, 322, 1002, 1824, 1972
Mox20I	TGGCCA	1 cut(s)	89
Mph1103I	ATGCAT	1 cut(s)	952
MroI	TCCGGA	1 cut(s)	769
MroNI	GCCGGC	1 cut(s)	5
MroXI	GAANNNNTTC	2 cut(s)	752, 1663
MscI	TGGCCA	1 cut(s)	89
MslI	CAYNNNNRTG	1 cut(s)	53
Msp20I	TGGCCA	1 cut(s)	89
MspA1I	CMGCKG	2 cut(s)	505, 2825
MspR9I	CCNGG	9 cut(s)	86, 393, 422, 598, 682, 969, 1379, 1571, 1678
MunI	CAATTG	2 cut(s)	1479, 2547
Mva1269I	GAATGC	2 cut(s)	460, 2007
MvaI	CCWGG	4 cut(s)	86, 422, 1571, 1678
MwoI	GCNNNNNNNGC	9 cut(s)	65, 277, 300, 646, 982, 1511, 1701, 2549, 2558
NaeI	GCCGGC	1 cut(s)	7
NarI	GGCGCC	1 cut(s)	678
NciI	CCSGG	5 cut(s)	393, 598, 682, 969, 1379
NdeII	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
NgoMIV	GCCGGC	1 cut(s)	5
NlaIII	CATG	9 cut(s)	118, 746, 997, 1083, 1358, 1727, 2445, 2518, 2612
NmeAIII	GCCGAG	1 cut(s)	710
NmuCI	GTSAC	5 cut(s)	1492, 1774, 2272, 2320, 2564
NsiI	ATGCAT	1 cut(s)	952
NspI	RCATGY	1 cut(s)	2612
PaeI	GCATGC	1 cut(s)	2612
PaeR7I	CTCGAG	1 cut(s)	2129
PaqCI	CACCTGC	1 cut(s)	1874
PceI	AGGCCT	2 cut(s)	237, 2416
PciSI	GCTCTTC	1 cut(s)	287
PctI	GAATGC	2 cut(s)	460, 2007
PdiI	GCCGGC	1 cut(s)	7
PdmI	GAANNNNTTC	2 cut(s)	752, 1663
PfeI	GAWTC	9 cut(s)	217, 511, 619, 748, 838, 1181, 1342, 1445, 1613
PflFI	GACNNNGTC	3 cut(s)	185, 206, 1953
PflMI	CCANNNNNTGG	1 cut(s)	898
PfoI	TCCNGGA	1 cut(s)	596
PkrI	GCNGC	7 cut(s)	504, 507, 530, 692, 723, 2440, 2485
PleI	GAGTC	5 cut(s)	949, 1167, 1726, 2738, 2804
PluTI	GGCGCC	1 cut(s)	681
PpsI	GAGTC	5 cut(s)	949, 1167, 1726, 2738, 2804
PpuMI	RGGWCCY	2 cut(s)	245, 902
PsiI	TTATAA	1 cut(s)	1124
Psp124BI	GAGCTC	1 cut(s)	282
Psp1406I	AACGTT	1 cut(s)	914
Psp5II	RGGWCCY	2 cut(s)	245, 902
Psp6I	CCWGG	4 cut(s)	84, 420, 1569, 1676
PspFI	CCCAGC	1 cut(s)	2585
PspGI	CCWGG	4 cut(s)	84, 420, 1569, 1676
PspOMI	GGGCCC	2 cut(s)	542, 1463
PspPPI	RGGWCCY	2 cut(s)	245, 902
PspXI	VCTCGAGB	1 cut(s)	2129
PstI	CTGCAG	1 cut(s)	510
PstNI	CAGNNNCTG	1 cut(s)	514
PsuI	RGATCY	4 cut(s)	802, 1270, 1603, 2264
PsyI	GACNNNGTC	3 cut(s)	185, 206, 1953
PvuII	CAGCTG	2 cut(s)	505, 2825
RigI	GGCCGGCC	1 cut(s)	9
RsaI	GTAC	3 cut(s)	356, 1153, 2432
RsaNI	GTAC	3 cut(s)	355, 1152, 2431
RseI	CAYNNNNRTG	1 cut(s)	53
SacI	GAGCTC	1 cut(s)	282
SapI	GCTCTTC	1 cut(s)	287
SatI	GCNGC	7 cut(s)	503, 506, 529, 691, 722, 2439, 2484
Sau3AI	GATC	8 cut(s)	705, 802, 1063, 1270, 1454, 1603, 1890, 2264
ScaI	AGTACT	1 cut(s)	2432
SchI	GAGTC	5 cut(s)	950, 1168, 1727, 2738, 2804
ScrFI	CCNGG	9 cut(s)	86, 393, 422, 598, 682, 969, 1379, 1571, 1678
SduI	GDGCHC	5 cut(s)	282, 546, 1467, 2035, 2130
SfaNI	GCATC	2 cut(s)	2053, 2547
SfcI	CTRYAG	2 cut(s)	506, 2689
SfoI	GGCGCC	1 cut(s)	679
Sfr274I	CTCGAG	1 cut(s)	2129
SinI	GGWCC	7 cut(s)	181, 223, 245, 317, 902, 1223, 1945
SlaI	CTCGAG	1 cut(s)	2129
SmiMI	CAYNNNNRTG	1 cut(s)	53
SmlI	CTYRAG	6 cut(s)	350, 446, 655, 2025, 2129, 2177
SmoI	CTYRAG	6 cut(s)	350, 446, 655, 2025, 2129, 2177
SphI	GCATGC	1 cut(s)	2612
SseBI	AGGCCT	2 cut(s)	237, 2416
SsiI	CCGC	7 cut(s)	66, 690, 693, 2552, 2630, 2699, 2807
SspDI	GGCGCC	1 cut(s)	677
SspI	AATATT	1 cut(s)	2675
SspMI	CTAG	6 cut(s)	233, 1245, 2072, 2121, 2558, 2762
SstI	GAGCTC	1 cut(s)	282
StuI	AGGCCT	2 cut(s)	237, 2416
StyD4I	CCNGG	9 cut(s)	84, 391, 420, 596, 680, 967, 1377, 1569, 1676
StyI	CCWWGG	3 cut(s)	232, 1905, 2109
TaaI	ACNGT	6 cut(s)	583, 1264, 1321, 1627, 1955, 2478
TaiI	ACGT	4 cut(s)	189, 521, 917, 2118
TaqI	TCGA	1 cut(s)	2130
TatI	WGTACW	1 cut(s)	2430
TauI	GCSGC	1 cut(s)	693
TfiI	GAWTC	9 cut(s)	217, 511, 619, 748, 838, 1181, 1342, 1445, 1613
TscAI	CASTG	5 cut(s)	60, 1711, 1872, 2573, 2578
TseFI	GTSAC	5 cut(s)	1492, 1774, 2272, 2320, 2564
TseI	GCWGC	6 cut(s)	502, 505, 528, 721, 2438, 2483
Tsp45I	GTSAC	5 cut(s)	1492, 1774, 2272, 2320, 2564
TspDTI	ATGAA	9 cut(s)	731, 1343, 1926, 2028, 2042, 2129, 2152, 2531, 2685
TspRI	CASTG	5 cut(s)	60, 1711, 1872, 2573, 2578
Tth111I	GACNNNGTC	3 cut(s)	185, 206, 1953
Van91I	CCANNNNNTGG	1 cut(s)	898
VpaK11BI	GGWCC	7 cut(s)	181, 223, 245, 317, 902, 1223, 1945
XagI	CCTNNNNNAGG	1 cut(s)	1071
XapI	RAATTY	6 cut(s)	121, 565, 877, 1307, 1783, 1913
XceI	RCATGY	1 cut(s)	2612
XhoI	CTCGAG	1 cut(s)	2129
XmaJI	CCTAGG	1 cut(s)	232
XmiI	GTMKAC	1 cut(s)	2782
XmnI	GAANNNNTTC	2 cut(s)	752, 1663
XspI	CTAG	6 cut(s)	233, 1245, 2072, 2121, 2558, 2762
ZraI	GACGTC	1 cut(s)	2116
ZrmI	AGTACT	1 cut(s)	2432
Zsp2I	ATGCAT	1 cut(s)	952

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.