Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003268

Physical Location & Seq

Genomic Coordinates Reverse (-)

17728402 .. 17732654

Gene Structure 4253 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1154600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2931 bp

ATGGAGAAAATATGGAGAAGGAGGAGGATATGCATTTTTGTAATTTTTTGTGTTTCCATGGTGGCTTATGGAGCCACTTTCCCAGGTGACCTGAAAATTCTGAATGATTTCAGAAAAGGGTTGGAGAATTCAGAGCTTTTGGAATGGCCAGACAATGGAAGTGACCCTTGTGGCCCTCCTTTATGGCCTCATGTGTACTGCAATGGGGACAGAGTCTCACAGATTCAGGTTCAGAAAATGGGACTCAAAGGTCCTTTGCCTCAGAACTTCAACCAGCTCTCTAAGCTCAGCAACCTTGGCCTCCAAAGGAACAACTTCAATGGGAAATTGCCTACCTTTAGAGGCTTATCAGAACTCGAATTCGCTTACTTGGATAATAATGATTTCGATACAATCCCTTTCGATTTCTTTGATGGCCTTAGCAGTCTTAGGGTTATGGCATTGGATCACATTCCGTTGAATGCGTCTACTGGATGGTCTTTGCCTAGTGAGTTGGCAAAATCTGTTCAATTGCAAAATCTGTCCTTGATTGAATGTAATTTGGCAGGACCGTTGCCGGAGTTTTTAGGGGCATTGCCGTCTCTCTCAGTCCTGCAACTTTCGTTTAATAGATTAACTGGTGTGATTCCGACAAGTTTTGGAGAGTCTTTGGTGCAGATATTGTGGTTGAACAATCAAGAAGGTGGACTGACCGGTCCTATTGACGTAATTGCATCAATGTCGTCGTTGACACAGGTTTGGCTGCATGGAAACCAATTCACCGGAACAATTCCAGCCAACATTGGAGATCTTTCTTCTCTCAAGGAACTCAACCTCAATGGCAACCAACTTGTTGGTCTGATTCCGCAGTCACTGGCGGACATGAAGCTTGACAAATTGGACCTGGGAAATAACCGGCTAATGGGTACAATACCGAAGTTTAAGTCCGGTAATGTTTCTTATAACTCTAATCCATTTTGTCAGACTGATCCTGGGCTGCAATGTGCCCCAGAAGTTACTGCACTTTTGGATTTTCTTGGTGATTTGAATTATCCATTAAGTCTTGCATCTGAGTGGTCGGGTAATAATCCGTGTGAGGGGCCTTGGTTGGGATTGAGTTGCAATCCTCAGACCAAGGTTTCCGTCATCAATTTGCCTAGACGCAAGATAATTGGTACTTTGAATCCTTCACTTGCAAAGTTAGATTCTTTGATTGATATAAGGCTTCCAGGAAACAACATAAGTGGTAAAGTTCCTACTAACTTTACTGAGTTGAAATCCTTGAGATTATTGGATATAAGCGGAAACAACATCGAGCCTCCTTTACCAAAATTCCGTGAGAGTCTGAAGGTCATCATCGACGGAAATCCTCTTTTAGTTGCTAATCAAACTGTTGCCCCTCCTCCTTTATCTAGAAGCCCACCTCCACTCAATAGTTCACAACCTCCATCAGACAGTCCATCAGATACCCCATTATCTGGTGGTGGAAAAACACCATTATCCCATAGTCCACCCTCACCAAGTACTCACTCGAGTCCTAATCCACCTGGTTCTGTCCAAGTGGATGTTCAGCCCCAAAATTCTAAGAAATCAAAAACAGTTATTATTGTGGCTGGAATCGCAGTTGTTTCTGTTGTGGCTCTTCTGATGATTTGTCTATTTATTTACTGCTGTAAGAATAGGAAAAAAATCTCAGAGGCTCCTACTTCCGTTGTGCTTCACCCTAGAGACCCATCTGATCCAGAAAATATGTATAAGATTGCGGTTTCGAGTAATACCACTGGAAGCTTGTCAACTAAAACTGGGACTACTGCCAGTAACTTCAGCAGTGGAATAGGGAACTCTCACATGGTTGAGGCTGGGAACCTTGTCATATCTGTTCAAATTCTTAGAAAGGTGACCAAAGATTTTGCACCCGAAAATGAGCTAGGCCATGGCGGATTTGGAACTGTTTACAAGGGTGAACTAGAAGATGGGACAAAAATAGCAGTTAAGAGAATGGAGGGTGGGGTAATCAGTAGCAAAGCATTGGATGAATTTCAGTCAGAAATTGCTGTTCTTTCCAAGGTTCGTCATCGACATTTGGTTTCTCTTTTGGGTTACTCTGTCGAAGGAAATGAAAGGCTTCTTGTCTACGAGTATATGTCTCAAGGTGCTCTTAGCAGGCATCTTTTTCATTGGAAGAGCTTCAATCTGAAGCCTTTATCTTGGACAAGGAGACTCAGTATTGTGTTGGACGTTGCAAGAGCGATGGAATACCTACATAATTTAGCCCGCCAGACATTCATACACCGAGATCTCAAATCTTCTAATATTCTTCTGGATGATGACTTTCATGCAAAAGTTTCAGATTTTGGGTTGGTGAAACTTGCTCCGGATGGGGAGAAGTCTGTTGCGACTAAGCTTGCAGGGACATTTGGATACCTCGCACCTGAATATGCCGTGATGGGGAAAATTACAACAAAAGCAGATGTGTTCAGCTTTGGAGTGGTGTTAATGGAACTTTTAACTGGATTAACGGCGCTTGATGAGAACCGGCCAGAGGAAAGCAGGTACTTGGCCGAGTGGTTTTGGCGAATAAAGTCAAACAAGGAGAAGCTTGTGGCTGCAATTGATCCAACTCTTGAAGTAAATGAAGAAAGTTTTGAGAGCATCTCAATAATAGCGGAACTGGCTGGTCATTGCACAGCGAGGGAACCAAGCCATCGGCCTGAAATGGGGCATGCAGTGAATGTGTTGTCCCTACTTGTCGAAAAGTGGAAACCTATTGAAGACGAATCTGATCACCTCTCAGGGATTGATTACAACCAACCTCTACCTCAGATGTTAAAGGTTTGGAAGGAATCAGAAAGCCAAGGAGTCAGCTATAATAGTCTCGAGGACAGCAAGGGCAGTATTCCTGCGAGGCCTAATGGATTTGCAGAGTCCTTCACTTCCGCAGATGGGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

977

Amino Acids

106.73

Weight (kDa)

6.08

Isoelectric Point (pI)

37.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	141 - 274	2.2e-06	Leucine-rich repeat region
LRR_8	PF13855	242 - 300	1.6e-07	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	633 - 903	1.8e-46	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	633 - 903	1.5e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003268)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g26630 FvH4_3g26630 FvH4_6g20300
malus_domestica	MD03G1154600.v1.1 MD12G1040200.v1.1 MD14G1038700.v1.1
prunus_persica	Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.6G143800_v2.0.a1 Prupe.7G094700_v2.0.a1
pyrus_communis	pycom03g10840 pycom12g03440 pycom14g03340
rosa_chinensis	RchiOBHm_Chr3g0475921 RchiOBHm_Chr5g0048201
rosa_laevigata	RLG00000023801 RLG00000034502
rosa_multiflora	Rmu_sc0001408.1_g000015 Rmu_sc0009168.1_g000004
rosa_roxburghii	Rroxscaffold_1G00033720 Rroxscaffold_6G00405730
rosa_rugosa	Rorug03G0151900 Rorug05G0237000 Rorug05G0237100
rosa_samantha	Rh3AG202700 Rh3BG232600 Rh3CG228000 Rh3DG228200 Rh5AG316200 Rh5BG326800 Rh5CG353000 Rh5DG337800
rosa_wichuraiana	Rw3G018440 Rw5G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	942
AasI	GACNNNNNNGTC	2 cut(s)	249, 693
Acc36I	ACCTGC	1 cut(s)	2520
AccB7I	CCANNNNNTGG	2 cut(s)	155, 1457
AccI	GTMKAC	2 cut(s)	467, 2112
AccIII	TCCGGA	1 cut(s)	2353
AciI	CCGC	8 cut(s)	845, 857, 1281, 1742, 1917, 2254, 2645, 2916
AclWI	GGATC	4 cut(s)	453, 962, 1712, 2588
AcoI	YGGCCR	3 cut(s)	146, 2516, 2538
AcsI	RAATTY	7 cut(s)	96, 127, 359, 1310, 1558, 1863, 2015
AcuI	CTGAAG	3 cut(s)	1346, 1786, 2195
AfaI	GTAC	5 cut(s)	197, 907, 1156, 1504, 2534
AfiI	CCNNNNNNNGG	9 cut(s)	155, 901, 1076, 1088, 1457, 2427, 2521, 2696, 2922
AgeI	ACCGGT	1 cut(s)	692
AjnI	CCWGG	5 cut(s)	82, 882, 970, 1207, 1525
AjuI	GAANNNNNNNTTGG	2 cut(s)	1530, 1562
Alw21I	GWGCWC	1 cut(s)	2137
Alw26I	GTCTC	6 cut(s)	220, 585, 1701, 2130, 2191, 2858
AlwI	GGATC	4 cut(s)	453, 962, 1712, 2588
AlwNI	CAGNNNCTG	1 cut(s)	853
Ama87I	CYCGRG	2 cut(s)	1510, 2855
Aor13HI	TCCGGA	1 cut(s)	2353
ApeKI	GCWGC	3 cut(s)	742, 976, 2585
ApoI	RAATTY	7 cut(s)	96, 127, 359, 1310, 1558, 1863, 2015
ArsI	GACNNNNNNTTYG	2 cut(s)	908, 940
AsiGI	ACCGGT	1 cut(s)	692
Asp700I	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
AspLEI	GCGC	1 cut(s)	2503
AspS9I	GGNCC	6 cut(s)	173, 251, 548, 695, 880, 1079
AsuHPI	GGTGA	9 cut(s)	98, 751, 1031, 1488, 1691, 1888, 1952, 2353, 2756
AvaI	CYCGRG	2 cut(s)	1510, 2855
AvaII	GGWCC	4 cut(s)	251, 548, 695, 880
BaeGI	GKGCMC	1 cut(s)	988
BalI	TGGCCA	1 cut(s)	148
BbsI	GAAGAC	1 cut(s)	2757
Bbv12I	GWGCWC	1 cut(s)	2137
BbvI	GCAGC	3 cut(s)	729, 963, 2572
BceAI	ACGGC	3 cut(s)	562, 2405, 2514
BcgI	CGANNNNNNTGC	2 cut(s)	702, 736
BciT130I	CCWGG	5 cut(s)	84, 884, 972, 1209, 1527
BciVI	GTATCC	1 cut(s)	2393
BclI	TGATCA	1 cut(s)	2761
BcoDI	GTCTC	6 cut(s)	220, 585, 1701, 2130, 2191, 2858
BfaI	CTAG	6 cut(s)	486, 1137, 1392, 1704, 1907, 1946
BfoI	RGCGCY	1 cut(s)	2504
BfuAI	ACCTGC	1 cut(s)	2520
BfuI	GTATCC	1 cut(s)	2393
BglII	AGATCT	2 cut(s)	787, 2275
BisI	GCNGC	3 cut(s)	743, 977, 2586
BlpI	GCTNAGC	1 cut(s)	287
BlsI	GCNGC	3 cut(s)	744, 978, 2587
BmcAI	AGTACT	1 cut(s)	1504
Bme1390I	CCNGG	5 cut(s)	84, 884, 972, 1209, 1527
Bme18I	GGWCC	4 cut(s)	251, 548, 695, 880
BmeT110I	CYCGRG	2 cut(s)	1510, 2855
BmgT120I	GGNCC	6 cut(s)	173, 251, 548, 695, 880, 1079
BmiI	GGNNCC	5 cut(s)	73, 1080, 1680, 1844, 2676
BmrFI	CCNGG	5 cut(s)	84, 884, 972, 1209, 1527
BmrI	ACTGGG	1 cut(s)	1791
BmsI	GCATC	4 cut(s)	722, 1055, 2155, 2640
BmuI	ACTGGG	1 cut(s)	1791
BpiI	GAAGAC	1 cut(s)	2757
BplI	GAGNNNNNCTC	2 cut(s)	2618, 2650
Bpu10I	CCTNAGC	1 cut(s)	419
Bpu1102I	GCTNAGC	1 cut(s)	287
BpuEI	CTTGAG	3 cut(s)	785, 1282, 2112
BsaI	GGTCTC	1 cut(s)	1701
BsaWI	WCCGGW	4 cut(s)	692, 761, 926, 2353
BsaXI	ACNNNNNCTCC	2 cut(s)	2188, 2218
Bsc4I	CCNNNNNNNGG	9 cut(s)	155, 901, 1076, 1088, 1457, 2427, 2521, 2696, 2922
Bse118I	RCCGGY	3 cut(s)	692, 894, 2514
Bse1I	ACTGG	8 cut(s)	475, 622, 858, 1765, 1786, 1794, 2494, 2655
Bse3DI	GCAATG	4 cut(s)	208, 572, 986, 2659
BseAI	TCCGGA	1 cut(s)	2353
BseBI	CCWGG	5 cut(s)	84, 884, 972, 1209, 1527
BseGI	GGATG	5 cut(s)	479, 1549, 2017, 2308, 2362
BseLI	CCNNNNNNNGG	9 cut(s)	155, 901, 1076, 1088, 1457, 2427, 2521, 2696, 2922
BseMI	GCAATG	4 cut(s)	208, 572, 986, 2659
BseNI	ACTGG	8 cut(s)	475, 622, 858, 1765, 1786, 1794, 2494, 2655
BseRI	GAGGAG	2 cut(s)	37, 1371
BseSI	GKGCMC	1 cut(s)	988
BseXI	GCAGC	3 cut(s)	729, 963, 2572
BseYI	CCCAGC	1 cut(s)	1838
BsgI	GTGCAG	2 cut(s)	674, 984
BshTI	ACCGGT	1 cut(s)	692
BsiHKAI	GWGCWC	1 cut(s)	2137
BsiHKCI	CYCGRG	2 cut(s)	1510, 2855
BsiSI	CCGG	7 cut(s)	557, 693, 762, 895, 927, 2354, 2515
BslFI	GGGAC	6 cut(s)	221, 255, 1798, 1969, 2404, 2704
BslI	CCNNNNNNNGG	9 cut(s)	155, 901, 1076, 1088, 1457, 2427, 2521, 2696, 2922
BsmAI	GTCTC	6 cut(s)	220, 585, 1701, 2130, 2191, 2858
BsmBI	CGTCTC	1 cut(s)	585
BsmFI	GGGAC	6 cut(s)	221, 255, 1798, 1969, 2404, 2704
BsmI	GAATGC	1 cut(s)	466
Bso31I	GGTCTC	1 cut(s)	1701
BsoBI	CYCGRG	2 cut(s)	1510, 2855
Bsp1286I	GDGCHC	2 cut(s)	988, 2137
Bsp13I	TCCGGA	1 cut(s)	2353
Bsp143I	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
Bsp1720I	GCTNAGC	1 cut(s)	287
Bsp19I	CCATGG	2 cut(s)	57, 1912
BspACI	CCGC	8 cut(s)	845, 857, 1281, 1742, 1917, 2254, 2645, 2916
BspEI	TCCGGA	1 cut(s)	2353
BspLI	GGNNCC	5 cut(s)	73, 1080, 1680, 1844, 2676
BspMI	ACCTGC	1 cut(s)	2520
BspPI	GGATC	4 cut(s)	453, 962, 1712, 2588
BspQI	GCTCTTC	2 cut(s)	1626, 2156
BspTNI	GGTCTC	1 cut(s)	1701
BsrDI	GCAATG	4 cut(s)	208, 572, 986, 2659
BsrFI	RCCGGY	3 cut(s)	692, 894, 2514
BsrI	ACTGG	8 cut(s)	475, 622, 858, 1765, 1786, 1794, 2494, 2655
BssAI	RCCGGY	3 cut(s)	692, 894, 2514
BssMI	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
BssT1I	CCWWGG	7 cut(s)	57, 295, 1082, 1113, 1912, 2043, 2833
Bst2UI	CCWGG	5 cut(s)	84, 884, 972, 1209, 1527
Bst4CI	ACNGT	5 cut(s)	552, 1372, 1436, 1579, 1930
Bst6I	CTCTTC	2 cut(s)	1626, 2156
BstC8I	GCNNGC	4 cut(s)	2144, 2254, 2385, 2703
BstDSI	CCRYGG	2 cut(s)	57, 1912
BstEII	GGTNACC	2 cut(s)	86, 1876
BstF5I	GGATG	5 cut(s)	479, 1549, 2017, 2308, 2362
BstH2I	RGCGCY	1 cut(s)	2504
BstHHI	GCGC	1 cut(s)	2503
BstKTI	GATC	7 cut(s)	448, 790, 970, 1720, 2278, 2596, 2764
BstMAI	GTCTC	6 cut(s)	220, 585, 1701, 2130, 2191, 2858
BstMBI	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
BstMWI	GCNNNNNNNGC	5 cut(s)	71, 283, 297, 1598, 2651
BstNI	CCWGG	5 cut(s)	84, 884, 972, 1209, 1527
BstNSI	RCATGY	1 cut(s)	2705
BstPI	GGTNACC	2 cut(s)	86, 1876
BstSCI	CCNGG	5 cut(s)	82, 882, 970, 1207, 1525
BstSLI	GKGCMC	1 cut(s)	988
BstV1I	GCAGC	3 cut(s)	729, 963, 2572
BstV2I	GAAGAC	1 cut(s)	2757
BstX2I	RGATCY	2 cut(s)	787, 2275
BstXI	CCANNNNNNTGG	1 cut(s)	833
BstYI	RGATCY	2 cut(s)	787, 2275
BsuI	GTATCC	1 cut(s)	2393
BtgI	CCRYGG	2 cut(s)	57, 1912
BtgZI	GCGATG	1 cut(s)	2243
BtsCI	GGATG	5 cut(s)	479, 1549, 2017, 2308, 2362
BtsI	GCAGTG	2 cut(s)	1813, 2712
BtsIMutI	CAGTG	4 cut(s)	851, 1758, 1813, 2712
BveI	ACCTGC	1 cut(s)	2520
Cac8I	GCNNGC	4 cut(s)	2144, 2254, 2385, 2703
CaiI	CAGNNNCTG	1 cut(s)	853
CfoI	GCGC	1 cut(s)	2503
Cfr10I	RCCGGY	3 cut(s)	692, 894, 2514
Cfr13I	GGNCC	6 cut(s)	173, 251, 548, 695, 880, 1079
CseI	GACGC	2 cut(s)	453, 1149
CsiI	ACCWGGT	1 cut(s)	1525
Csp6I	GTAC	5 cut(s)	196, 906, 1155, 1503, 2533
CspAI	ACCGGT	1 cut(s)	692
CspCI	CAANNNNNGTGG	2 cut(s)	1204, 1239
CviAII	CATG	8 cut(s)	58, 191, 746, 862, 1828, 1913, 2315, 2702
CviQI	GTAC	5 cut(s)	196, 906, 1155, 1503, 2533
DpnI	GATC	7 cut(s)	447, 789, 969, 1719, 2277, 2595, 2763
DpnII	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
DrdI	GACNNNNNNGTC	2 cut(s)	249, 693
DseDI	GACNNNNNNGTC	2 cut(s)	249, 693
EaeI	YGGCCR	3 cut(s)	146, 2516, 2538
Eam1104I	CTCTTC	2 cut(s)	1626, 2156
EarI	CTCTTC	2 cut(s)	1626, 2156
EciI	GGCGGA	2 cut(s)	872, 1932
Eco130I	CCWWGG	7 cut(s)	57, 295, 1082, 1113, 1912, 2043, 2833
Eco147I	AGGCCT	1 cut(s)	2887
Eco31I	GGTCTC	1 cut(s)	1701
Eco47I	GGWCC	4 cut(s)	251, 548, 695, 880
Eco57I	CTGAAG	3 cut(s)	1346, 1786, 2195
Eco88I	CYCGRG	2 cut(s)	1510, 2855
Eco91I	GGTNACC	2 cut(s)	86, 1876
EcoO109I	RGGNCCY	2 cut(s)	251, 1079
EcoO65I	GGTNACC	2 cut(s)	86, 1876
EcoRI	GAATTC	2 cut(s)	127, 359
EcoRII	CCWGG	5 cut(s)	82, 882, 970, 1207, 1525
EcoT14I	CCWWGG	7 cut(s)	57, 295, 1082, 1113, 1912, 2043, 2833
EcoT22I	ATGCAT	1 cut(s)	35
ErhI	CCWWGG	7 cut(s)	57, 295, 1082, 1113, 1912, 2043, 2833
Esp3I	CGTCTC	1 cut(s)	585
FaeI	CATG	8 cut(s)	61, 194, 749, 865, 1831, 1916, 2318, 2705
FalI	AAGNNNNNCTT	4 cut(s)	151, 183, 2121, 2153
FaqI	GGGAC	6 cut(s)	221, 255, 1798, 1969, 2404, 2704
FatI	CATG	8 cut(s)	57, 190, 745, 861, 1827, 1912, 2314, 2701
FauI	CCCGC	1 cut(s)	2261
FbaI	TGATCA	1 cut(s)	2761
FblI	GTMKAC	2 cut(s)	467, 2112
Fnu4HI	GCNGC	3 cut(s)	743, 977, 2586
FokI	GGATG	5 cut(s)	486, 1556, 2024, 2315, 2369
Fsp4HI	GCNGC	3 cut(s)	743, 977, 2586
FspBI	CTAG	6 cut(s)	486, 1137, 1392, 1704, 1907, 1946
GlaI	GCGC	1 cut(s)	2502
GluI	GCNGC	3 cut(s)	743, 977, 2586
GsaI	CCCAGC	1 cut(s)	1842
HaeII	RGCGCY	1 cut(s)	2504
HapII	CCGG	7 cut(s)	557, 693, 762, 895, 927, 2354, 2515
HgaI	GACGC	2 cut(s)	453, 1149
HhaI	GCGC	1 cut(s)	2503
Hin1II	CATG	8 cut(s)	61, 194, 749, 865, 1831, 1916, 2318, 2705
Hin6I	GCGC	1 cut(s)	2501
HinP1I	GCGC	1 cut(s)	2501
HincII	GTYRAC	2 cut(s)	729, 1773
HindII	GTYRAC	2 cut(s)	729, 1773
HindIII	AAGCTT	4 cut(s)	866, 1765, 2381, 2576
HpaII	CCGG	7 cut(s)	557, 693, 762, 895, 927, 2354, 2515
HphI	GGTGA	9 cut(s)	98, 751, 1031, 1488, 1691, 1888, 1952, 2353, 2756
Hpy188III	TCNNGA	7 cut(s)	677, 1392, 1721, 2300, 2354, 2603, 2855
Hpy99I	CGWCG	2 cut(s)	727, 1343
HpyAV	CCTTC	7 cut(s)	12, 674, 1176, 1321, 2084, 2812, 2917
HpyCH4III	ACNGT	5 cut(s)	552, 1372, 1436, 1579, 1930
HpyCH4IV	ACGT	2 cut(s)	705, 2217
HpyF10VI	GCNNNNNNNGC	5 cut(s)	71, 283, 297, 1598, 2651
HpySE526I	ACGT	2 cut(s)	705, 2217
Hsp92II	CATG	8 cut(s)	61, 194, 749, 865, 1831, 1916, 2318, 2705
HspAI	GCGC	1 cut(s)	2501
Kpn2I	TCCGGA	1 cut(s)	2353
Ksp22I	TGATCA	1 cut(s)	2761
Kzo9I	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
LguI	GCTCTTC	2 cut(s)	1626, 2156
LmnI	GCTCC	3 cut(s)	71, 1684, 2356
Lsp1109I	GCAGC	3 cut(s)	729, 963, 2572
LweI	GCATC	4 cut(s)	722, 1055, 2155, 2640
MabI	ACCWGGT	1 cut(s)	1525
MaeI	CTAG	6 cut(s)	486, 1137, 1392, 1704, 1907, 1946
MaeII	ACGT	2 cut(s)	705, 2217
MaeIII	GTNAC	7 cut(s)	86, 161, 849, 994, 1796, 1876, 2078
MalI	GATC	7 cut(s)	447, 789, 969, 1719, 2277, 2595, 2763
MboI	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
MboII	GAAGA	8 cut(s)	786, 1613, 1961, 2173, 2277, 2288, 2627, 2762
MfeI	CAATTG	2 cut(s)	509, 2589
MflI	RGATCY	2 cut(s)	787, 2275
MhlI	GDGCHC	2 cut(s)	988, 2137
MlsI	TGGCCA	1 cut(s)	148
MluNI	TGGCCA	1 cut(s)	148
MlyI	GAGTC	8 cut(s)	222, 237, 653, 1330, 1522, 2193, 2847, 2912
MmeI	TCCRAC	4 cut(s)	102, 653, 2193, 2621
Mox20I	TGGCCA	1 cut(s)	148
Mph1103I	ATGCAT	1 cut(s)	35
MroI	TCCGGA	1 cut(s)	2353
MroXI	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
MscI	TGGCCA	1 cut(s)	148
MseI	TTAA	9 cut(s)	606, 614, 921, 1037, 1971, 2474, 2486, 2495, 2807
MslI	CAYNNNNRTG	1 cut(s)	1051
Msp20I	TGGCCA	1 cut(s)	148
MspI	CCGG	7 cut(s)	557, 693, 762, 895, 927, 2354, 2515
MspR9I	CCNGG	5 cut(s)	84, 884, 972, 1209, 1527
MunI	CAATTG	2 cut(s)	509, 2589
Mva1269I	GAATGC	1 cut(s)	466
MvaI	CCWGG	5 cut(s)	84, 884, 972, 1209, 1527
MwoI	GCNNNNNNNGC	5 cut(s)	71, 283, 297, 1598, 2651
NcoI	CCATGG	2 cut(s)	57, 1912
NdeII	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
NlaIII	CATG	8 cut(s)	61, 194, 749, 865, 1831, 1916, 2318, 2705
NlaIV	GGNNCC	5 cut(s)	73, 1080, 1680, 1844, 2676
NmeAIII	GCCGAG	1 cut(s)	2566
NmuCI	GTSAC	4 cut(s)	86, 161, 849, 1876
NsiI	ATGCAT	1 cut(s)	35
NspI	RCATGY	1 cut(s)	2705
PaeI	GCATGC	1 cut(s)	2705
PaeR7I	CTCGAG	2 cut(s)	1510, 2855
PceI	AGGCCT	1 cut(s)	2887
PciSI	GCTCTTC	2 cut(s)	1626, 2156
PctI	GAATGC	1 cut(s)	466
PdmI	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
PfeI	GAWTC	8 cut(s)	223, 625, 841, 1162, 1184, 1596, 2756, 2822
PflFI	GACNNNGTC	1 cut(s)	212
PflMI	CCANNNNNTGG	2 cut(s)	155, 1457
PfoI	TCCNGGA	1 cut(s)	1207
PinAI	ACCGGT	1 cut(s)	692
PkrI	GCNGC	3 cut(s)	744, 978, 2587
PleI	GAGTC	8 cut(s)	221, 237, 652, 1329, 1521, 2193, 2846, 2911
PpsI	GAGTC	8 cut(s)	221, 237, 652, 1329, 1521, 2193, 2846, 2911
PpuMI	RGGWCCY	1 cut(s)	251
PsiI	TTATAA	1 cut(s)	942
Psp5II	RGGWCCY	1 cut(s)	251
Psp6I	CCWGG	5 cut(s)	82, 882, 970, 1207, 1525
PspEI	GGTNACC	2 cut(s)	86, 1876
PspFI	CCCAGC	1 cut(s)	1838
PspGI	CCWGG	5 cut(s)	82, 882, 970, 1207, 1525
PspN4I	GGNNCC	5 cut(s)	73, 1080, 1680, 1844, 2676
PspPI	GGNCC	6 cut(s)	173, 251, 548, 695, 880, 1079
PspPPI	RGGWCCY	1 cut(s)	251
PspXI	VCTCGAGB	1 cut(s)	1510
PstNI	CAGNNNCTG	1 cut(s)	853
PsuI	RGATCY	2 cut(s)	787, 2275
PsyI	GACNNNGTC	1 cut(s)	212
RsaI	GTAC	5 cut(s)	197, 907, 1156, 1504, 2534
RsaNI	GTAC	5 cut(s)	196, 906, 1155, 1503, 2533
RseI	CAYNNNNRTG	1 cut(s)	1051
SapI	GCTCTTC	2 cut(s)	1626, 2156
SaqAI	TTAA	9 cut(s)	606, 614, 921, 1037, 1971, 2474, 2486, 2495, 2807
SatI	GCNGC	3 cut(s)	743, 977, 2586
Sau3AI	GATC	7 cut(s)	445, 787, 967, 1717, 2275, 2593, 2761
Sau96I	GGNCC	6 cut(s)	173, 251, 548, 695, 880, 1079
ScaI	AGTACT	1 cut(s)	1504
SchI	GAGTC	8 cut(s)	222, 237, 653, 1330, 1522, 2193, 2847, 2912
ScrFI	CCNGG	5 cut(s)	84, 884, 972, 1209, 1527
SduI	GDGCHC	2 cut(s)	988, 2137
SexAI	ACCWGGT	1 cut(s)	1525
SfaNI	GCATC	4 cut(s)	722, 1055, 2155, 2640
Sfr274I	CTCGAG	2 cut(s)	1510, 2855
SinI	GGWCC	4 cut(s)	251, 548, 695, 880
SlaI	CTCGAG	2 cut(s)	1510, 2855
SmiMI	CAYNNNNRTG	1 cut(s)	1051
SmlI	CTYRAG	5 cut(s)	800, 1261, 1510, 2127, 2855
SmoI	CTYRAG	5 cut(s)	800, 1261, 1510, 2127, 2855
SphI	GCATGC	1 cut(s)	2705
SseBI	AGGCCT	1 cut(s)	2887
SsiI	CCGC	8 cut(s)	845, 857, 1281, 1742, 1917, 2254, 2645, 2916
SspI	AATATT	1 cut(s)	2293
SspMI	CTAG	6 cut(s)	486, 1137, 1392, 1704, 1907, 1946
StuI	AGGCCT	1 cut(s)	2887
StyD4I	CCNGG	5 cut(s)	82, 882, 970, 1207, 1525
StyI	CCWWGG	7 cut(s)	57, 295, 1082, 1113, 1912, 2043, 2833
TaaI	ACNGT	5 cut(s)	552, 1372, 1436, 1579, 1930
TaiI	ACGT	2 cut(s)	708, 2220
TatI	WGTACW	2 cut(s)	195, 1502
TfiI	GAWTC	8 cut(s)	223, 625, 841, 1162, 1184, 1596, 2756, 2822
Tru1I	TTAA	9 cut(s)	606, 614, 921, 1037, 1971, 2474, 2486, 2495, 2807
Tru9I	TTAA	9 cut(s)	606, 614, 921, 1037, 1971, 2474, 2486, 2495, 2807
TscAI	CASTG	4 cut(s)	858, 1765, 1813, 2712
TseFI	GTSAC	4 cut(s)	86, 161, 849, 1876
TseI	GCWGC	3 cut(s)	742, 976, 2585
Tsp45I	GTSAC	4 cut(s)	86, 161, 849, 1876
TspDTI	ATGAA	7 cut(s)	878, 2028, 2112, 2144, 2254, 2303, 2628
TspGWI	ACGGA	6 cut(s)	444, 1059, 1111, 1304, 1356, 1678
TspRI	CASTG	4 cut(s)	858, 1765, 1813, 2712
Tth111I	GACNNNGTC	1 cut(s)	212
Van91I	CCANNNNNTGG	2 cut(s)	155, 1457
VpaK11BI	GGWCC	4 cut(s)	251, 548, 695, 880
XapI	RAATTY	7 cut(s)	96, 127, 359, 1310, 1558, 1863, 2015
XbaI	TCTAGA	1 cut(s)	1391
XceI	RCATGY	1 cut(s)	2705
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1919
XhoI	CTCGAG	2 cut(s)	1510, 2855
XmiI	GTMKAC	2 cut(s)	467, 2112
XmnI	GAANNNNTTC	4 cut(s)	107, 314, 2103, 2165
XspI	CTAG	6 cut(s)	486, 1137, 1392, 1704, 1907, 1946
ZrmI	AGTACT	1 cut(s)	1504
Zsp2I	ATGCAT	1 cut(s)	35

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.