MD12G1040200.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003268
Physical Location & Seq
Reverse (-)
4368152 .. 4371590
3439 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1040200.v1.1.491

Sequence Viewer

Length: 2844 bp
ATGGAAAAACACCATAGTGCTGCTGCAGAACATGCTCTCTCACTCCTCCTCCTGCTTCTCTCACTAGCTTCACTTGCTCTGAGCGCCACAGACCCGAACGACCTTGCAATCCTCAACCAGCTGAGAAAAAATCTAGAAAACCCAGAGCTCTTGAAGTGGCCGGAAAATGGCGACGACCCATGTGGGGCCAGGTGGGCCCACGTCTTCTGCGCCGGTTCGAGAGTGTCCCAGATTCAGGTCCAGAACCTTGGCCTGAAGGGTCCTCTGCCCCTGAACTTCAACCAGCTCACTGAGCTCACAAACATTGGCCTCCAGAGGAACCACTTCAGTGGGCCCCTCCCTTCCCTCAAGGGGCTGTCGAAGCTCCGGTTTGCTTACTTGGATTTCAACGACTTCACTTCGATTCCTGTCGACTTCTTCGAAGGTCTCGATTCTCTGGAGGTTTTGGCTTTGGACGACAACAATTTGAATGCCACGACAGGCTGGAGTTTCCCTCCTCAGCTGGGGAACTCGGCGCAGTTGCAGAATCTTAGTTGCATGAGTTGTAATCTGGTCGGTCCATTGCCCCATTTTCTCGGGAACATGTCGTCCCTAACGGTTTTGCAACTCTCGGGTAACGGACTGTCCGGCGGGATTCCGCCCAGCTTTAACGGCCTTAATTTGCAGATACTTTGGCTGAACAATCCGACCGGCGGCGGATTGAGTGGTCCGATTGATGTTCTGACCACAATGGTGCAGCTCAACAGCGTGTGGCTCCACGGGAATCAGTTTTCCGGCGTGATTCCGAACAGCATTGGTAATTTAACATCTTTGAAAGATCTTAATCTTAATCAAAACCAGTTTGTTGGTTTAGTTCCTGATGGTTTGGCTAATTTAGCACTTGATAGTTTGACCTTGAACAATAATCATTTGATGGGTCCAATCCCGAAATTCAAAGCTCGCAATGCGAGTTTTGATTCGAATGCGTTTTGTCAGTCCACTCCCGGGGTTCCTTGCTCCGCGGAGGTTATGGCGCTTATCGAGTTCCTTAGCGGGTTGAATTACCCTTCAACGCTTGTTTCTAAATGGTCTGGTAATGATCCGTGCCGGTCGTGGTTGGGAGTGAGTTGTGGGAACGATGGGAAGGTGTCTGTTATAAATCTGCCCAAGTATAATCTGAATGGTACATTGAGTCATTCGGTTGCGAAGTTAGAATCTCTTGTTCAAATTAGGCTTCAGAACAACAATTTGTGGGGTTCAGTTCCTGAAAATTGGACTAGCTTGAAATCCTTGACTGAGTTGGATCTTAGTGGGAACAATATTTCCCCTCCATTGCCGAAATTCAGTAGCATTGTCAAAGTTGCCGTTGATGGGAATCGTTTGTTTAATGGTAATCCATCTGCGGCAGGGGCAGCACCAGAAGATAGCCCTTCCTCATCCACAGATTCAGGTTCTCATGTCAATGGTACTTCTCAACTGAACCAACATAAAGCATTGAAAAGGTCTAGCACTGTTTCTTTTGTAGCTCCGGTTGCAAGTGTTGCTGTTATTGCGTTTTTGCTTGTAATTCCTTTATCTATGTACTGTAAGAAGAGAAGAGATGCATTCAAGAATTCAACTTCCCATGTAATTCACCCGAGAGATGCATCTGATTCGGATAGTATGGTTAAGGTTGTTGTTGCCAGCAATACCAATGGAAGCGCTTCTACCGTTACAGGGAGCTCTGCAAGCAGAAACAGTAGTGGAATAGGTGAGTCCCATGTCATTGAAGCGGGGAATCTCATCATATCAGTTCAAGTTCTTCGAAACGTGACAAACAATTTTGCCCCAGAAAATGAGCTAGGCCGTGGTGGCTTTGGGGTGGTTTATAAGGGAGAACTGGATGACGGTACAAAAATAGCAGTAAAAAGAATGGAGGCTGGTGTGATTAGCAACAAAGCCTTGGATGAATTCCAGGCCGAAATTGCAGTTCTGTCAAAGGTCAGACACCGTCATTTGGTATCGCTTCTGGGGTATTCCGTTGAAGGAAACGAGAGAATTCTTGTCTATGAGTATATGCCTCAGGGGGCTCTAAGCAGGCATCTTTTCCGTTGGAAGACCTTCGAATTAGAGCCGCTCTCTTGGAAGAGGAGGCTAAACATTGCCTTGGATGTTGCTAGAGGGATGGATTATCTTCACAATCTAGCTCACAAAAGCTTCATACACAGAGATCTTAAATCATCAAATATCTTACTGGGTGATGATTTTAAAGCAAAAGTTTCAGATTTTGGATTGGTGAAACTGGCTCCTGATGGTGAAAGATCCGTTGTGACCAGGCTTGCCGGGACTTTCGGGTACTTAGCACCGGAGTATGCTGTGACGGGAAAAATTACAACGAAAGCAGATGTCTTCAGTTTCGGAGTTGTGTTAATGGAGCTGTTAACTGGAATGATGGCACTAGATGAGGATAGACCTGAAGAAAGTCAATACTTGGCTGCATGGTTCTGGCACATAAAATCGAATAAGGAGAAACTCATGGCTGCTATCGACCCAACTCTTGACAGAAATGAAGAAACATTTGAGAGCATCGCCATAATTGCAGAACTCGCCGGGCACTGCACTGCAAGGGAACCCAGCCAAAGACCGGATATGAGCCATGCCGTGAATGTTTTGTCCCCGCTTGTTGAAAAATGGAAACCTATCGATGATGAGAACGAGGAGTATTCTGGGATTGATTATAGCCTGCCGCTTAACCAGATGGTGAAGGGCTGGCAGGACGCAGAAGGAAAGGACTCCAGTTATCTGAACCTGGAAGACAGCAAGGGGAGTATACCGGCAAGGCCAACTGGCTTTGCAGAGTCTTTTACTTCCGCTGATGGTCGGTAA

Protein Analysis

948

Amino Acids

102.65

Weight (kDa)

5.83

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 246 - 304 1e-05 Leucine rich repeat
LRRNT_2 PF08263 334 - 370 2.6e-07 Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr PF07714 604 - 876 4.3e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 604 - 875 1.6e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1136, 1848
AccBSI CCGCTC 1 cut(s) 2095
AccI GTMKAC 2 cut(s) 411, 2788
AccII CGCG 1 cut(s) 1001
AclWI GGATC 3 cut(s) 1073, 1290, 2274
AcoI YGGCCR 1 cut(s) 158
AcsI RAATTY 5 cut(s) 929, 1319, 1591, 1928, 2016
AcuI CTGAAG 5 cut(s) 275, 310, 1199, 2353, 2454
AfaI GTAC 5 cut(s) 1165, 1447, 1562, 1870, 2315
AfeI AGCGCT 1 cut(s) 1681
AflIII ACRYGT 1 cut(s) 582
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 4 cut(s) 188, 1932, 2291, 2766
AleI CACNNNNGTG 3 cut(s) 15, 327, 731
AloI GAACNNNNNNTCC 4 cut(s) 572, 604, 1286, 1318
Alw21I GWGCWC 3 cut(s) 150, 297, 1703
Alw26I GTCTC 1 cut(s) 431
AlwI GGATC 3 cut(s) 1073, 1290, 2274
AlwNI CAGNNNCTG 1 cut(s) 1244
Ama87I CYCGRG 4 cut(s) 575, 610, 983, 1615
Aor51HI AGCGCT 1 cut(s) 1681
ApaI GGGCCC 2 cut(s) 199, 336
ApeKI GCWGC 6 cut(s) 20, 23, 736, 1391, 2453, 2498
ApoI RAATTY 5 cut(s) 929, 1319, 1591, 1928, 2016
Asp700I GAANNNNTTC 3 cut(s) 323, 1681, 2078
AspLEI GCGC 5 cut(s) 86, 212, 517, 1015, 1682
AsuC2I CCSGG 4 cut(s) 984, 985, 2302, 2569
AsuHPI GGTGA 6 cut(s) 1604, 1742, 2228, 2266, 2285, 2731
AsuII TTCGAA 4 cut(s) 420, 959, 1783, 2082
AvaI CYCGRG 4 cut(s) 575, 610, 983, 1615
AvaII GGWCC 5 cut(s) 238, 260, 557, 707, 917
AxyI CCTNAGG 1 cut(s) 2040
BaeGI GKGCMC 3 cut(s) 199, 336, 2574
BanII GRGCYC 6 cut(s) 150, 199, 297, 336, 1703, 2050
BarI GAAGNNNNNNTAC 2 cut(s) 1669, 1701
BbsI GAAGAC 4 cut(s) 196, 2081, 2359, 2778
Bbv12I GWGCWC 3 cut(s) 150, 297, 1703
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 6 cut(s) 7, 10, 748, 1403, 2440, 2485
BceAI ACGGC 4 cut(s) 667, 1328, 1809, 2603
BcgI CGANNNNNNTGC 2 cut(s) 1614, 1648
BciT130I CCWGG 4 cut(s) 190, 1934, 2293, 2768
BcnI CCSGG 4 cut(s) 984, 985, 2302, 2569
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 8 cut(s) 65, 134, 1257, 1485, 1820, 2136, 2162, 2417
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 3 cut(s) 87, 1016, 1683
BglI GCCNNNNNGGC 2 cut(s) 194, 1830
BglII AGATCT 2 cut(s) 817, 2188
Bme1390I CCNGG 8 cut(s) 190, 984, 985, 1934, 2293, 2302, 2569, 2768
Bme18I GGWCC 5 cut(s) 238, 260, 557, 707, 917
BmeT110I CYCGRG 4 cut(s) 575, 610, 983, 1615
BmgBI CACGTC 1 cut(s) 202
BmrFI CCNGG 8 cut(s) 190, 984, 985, 1934, 2293, 2302, 2569, 2768
BmrI ACTGGG 1 cut(s) 2222
BmsI GCATC 5 cut(s) 1570, 1612, 1634, 2068, 2553
BmuI ACTGGG 1 cut(s) 2222
BpiI GAAGAC 4 cut(s) 196, 2081, 2359, 2778
BplI GAGNNNNNCTC 4 cut(s) 478, 510, 2081, 2113
BpmI CTGGAG 4 cut(s) 296, 458, 505, 2737
Bpu10I CCTNAGC 2 cut(s) 498, 1028
Bpu14I TTCGAA 4 cut(s) 420, 959, 1783, 2082
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 4 cut(s) 984, 985, 2302, 2569
Bsa29I ATCGAT 1 cut(s) 2661
BsaBI GATNNNNATC 2 cut(s) 822, 1353
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 8 cut(s) 247, 757, 983, 984, 999, 1825, 1920, 2124
BsaWI WCCGGW 4 cut(s) 366, 1507, 2323, 2602
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bse118I RCCGGY 4 cut(s) 212, 689, 1086, 2791
Bse1I ACTGG 7 cut(s) 838, 1863, 2217, 2265, 2407, 2754, 2809
Bse21I CCTNAGG 1 cut(s) 2040
Bse3DI GCAATG 4 cut(s) 560, 949, 1310, 2118
Bse8I GATNNNNATC 2 cut(s) 822, 1353
BseBI CCWGG 4 cut(s) 190, 1934, 2293, 2768
BseCI ATCGAT 1 cut(s) 2661
BseDI CCNNGG 8 cut(s) 247, 757, 983, 984, 999, 1825, 1920, 2124
BseGI GGATG 5 cut(s) 1415, 1867, 1930, 2134, 2148
BseJI GATNNNNATC 2 cut(s) 822, 1353
BseMI GCAATG 4 cut(s) 560, 949, 1310, 2118
BseMII CTCAG 6 cut(s) 71, 113, 282, 512, 1266, 2054
BseNI ACTGG 7 cut(s) 838, 1863, 2217, 2265, 2407, 2754, 2809
BseRI GAGGAG 5 cut(s) 35, 38, 486, 2122, 2690
BseSI GKGCMC 3 cut(s) 199, 336, 2574
BseXI GCAGC 6 cut(s) 7, 10, 748, 1403, 2440, 2485
BseYI CCCAGC 3 cut(s) 502, 641, 2591
BsgI GTGCAG 2 cut(s) 755, 2560
Bsh1236I CGCG 1 cut(s) 1001
Bsh1285I CGRYCG 2 cut(s) 690, 1091
BshVI ATCGAT 1 cut(s) 2661
BsiEI CGRYCG 2 cut(s) 690, 1091
BsiHKAI GWGCWC 3 cut(s) 150, 297, 1703
BsiHKCI CYCGRG 4 cut(s) 575, 610, 983, 1615
BslFI GGGAC 5 cut(s) 211, 574, 1720, 2317, 2617
BsmAI GTCTC 1 cut(s) 431
BsmFI GGGAC 5 cut(s) 211, 574, 1720, 2317, 2617
BsmI GAATGC 3 cut(s) 475, 967, 1583
Bso31I GGTCTC 1 cut(s) 431
BsoBI CYCGRG 4 cut(s) 575, 610, 983, 1615
Bsp119I TTCGAA 4 cut(s) 420, 959, 1783, 2082
Bsp120I GGGCCC 2 cut(s) 195, 332
Bsp1286I GDGCHC 7 cut(s) 150, 199, 297, 336, 1703, 2050, 2574
Bsp143I GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
BspCNI CTCAG 6 cut(s) 72, 114, 283, 511, 1267, 2053
BspDI ATCGAT 1 cut(s) 2661
BspFNI CGCG 1 cut(s) 1001
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 3 cut(s) 1073, 1290, 2274
BspT104I TTCGAA 4 cut(s) 420, 959, 1783, 2082
BspTNI GGTCTC 1 cut(s) 431
BsrBI CCGCTC 1 cut(s) 2095
BsrDI GCAATG 4 cut(s) 560, 949, 1310, 2118
BsrFI RCCGGY 4 cut(s) 212, 689, 1086, 2791
BsrI ACTGG 7 cut(s) 838, 1863, 2217, 2265, 2407, 2754, 2809
BssAI RCCGGY 4 cut(s) 212, 689, 1086, 2791
BssECI CCNNGG 8 cut(s) 247, 757, 983, 984, 999, 1825, 1920, 2124
BssMI GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
BssNAI GTATAC 1 cut(s) 2789
BssT1I CCWWGG 3 cut(s) 247, 1920, 2124
Bst1107I GTATAC 1 cut(s) 2789
Bst2UI CCWGG 4 cut(s) 190, 1934, 2293, 2768
Bst4CI ACNGT 8 cut(s) 598, 624, 1492, 1565, 1690, 1718, 1868, 1970
Bst6I CTCTTC 3 cut(s) 1565, 1570, 2099
BstAPI GCANNNNNTGC 2 cut(s) 32, 1520
BstBI TTCGAA 4 cut(s) 420, 959, 1783, 2082
BstC8I GCNNGC 7 cut(s) 940, 1663, 1708, 2057, 2298, 2702, 2729
BstDSI CCRYGG 3 cut(s) 757, 999, 1825
BstF5I GGATG 5 cut(s) 1415, 1867, 1930, 2134, 2148
BstFNI CGCG 1 cut(s) 1001
BstH2I RGCGCY 3 cut(s) 87, 1016, 1683
BstHHI GCGC 5 cut(s) 86, 212, 517, 1015, 1682
BstKTI GATC 5 cut(s) 820, 1081, 1285, 2191, 2282
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
BstMCI CGRYCG 2 cut(s) 690, 1091
BstNI CCWGG 4 cut(s) 190, 1934, 2293, 2768
BstNSI RCATGY 2 cut(s) 35, 586
BstSCI CCNGG 8 cut(s) 188, 982, 983, 1932, 2291, 2300, 2567, 2766
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 3 cut(s) 199, 336, 2574
BstUI CGCG 1 cut(s) 1001
BstV1I GCAGC 6 cut(s) 7, 10, 748, 1403, 2440, 2485
BstV2I GAAGAC 4 cut(s) 196, 2081, 2359, 2778
BstX2I RGATCY 4 cut(s) 817, 1282, 2188, 2279
BstXI CCANNNNNNTGG 3 cut(s) 248, 329, 845
BstYI RGATCY 4 cut(s) 817, 1282, 2188, 2279
BstZ17I GTATAC 1 cut(s) 2789
Bsu15I ATCGAT 1 cut(s) 2661
Bsu36I CCTNAGG 1 cut(s) 2040
BsuTUI ATCGAT 1 cut(s) 2661
BtgI CCRYGG 3 cut(s) 757, 999, 1825
BtgZI GCGATG 1 cut(s) 2530
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 5 cut(s) 1415, 1867, 1930, 2134, 2148
BtsI GCAGTG 2 cut(s) 2572, 2577
BtsIMutI CAGTG 5 cut(s) 288, 334, 1488, 2572, 2577
Cac8I GCNNGC 7 cut(s) 940, 1663, 1708, 2057, 2298, 2702, 2729
CaiI CAGNNNCTG 1 cut(s) 1244
CfoI GCGC 5 cut(s) 86, 212, 517, 1015, 1682
Cfr10I RCCGGY 4 cut(s) 212, 689, 1086, 2791
Cfr42I CCGCGG 1 cut(s) 1002
Cfr9I CCCGGG 1 cut(s) 983
ClaI ATCGAT 1 cut(s) 2661
CseI GACGC 1 cut(s) 2744
Csp6I GTAC 5 cut(s) 1164, 1446, 1561, 1869, 2314
CviQI GTAC 5 cut(s) 1164, 1446, 1561, 1869, 2314
DpnI GATC 5 cut(s) 819, 1080, 1284, 2190, 2281
DpnII GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
DraI TTTAAA 1 cut(s) 2227
EaeI YGGCCR 1 cut(s) 158
Eam1104I CTCTTC 3 cut(s) 1565, 1570, 2099
EarI CTCTTC 3 cut(s) 1565, 1570, 2099
EciI GGCGGA 2 cut(s) 627, 711
Ecl136II GAGCTC 3 cut(s) 148, 295, 1701
Eco130I CCWWGG 3 cut(s) 247, 1920, 2124
Eco24I GRGCYC 6 cut(s) 150, 199, 297, 336, 1703, 2050
Eco31I GGTCTC 1 cut(s) 431
Eco47I GGWCC 5 cut(s) 238, 260, 557, 707, 917
Eco47III AGCGCT 1 cut(s) 1681
Eco53kI GAGCTC 3 cut(s) 148, 295, 1701
Eco57I CTGAAG 5 cut(s) 275, 310, 1199, 2353, 2454
Eco81I CCTNAGG 1 cut(s) 2040
Eco88I CYCGRG 4 cut(s) 575, 610, 983, 1615
EcoICRI GAGCTC 3 cut(s) 148, 295, 1701
EcoO109I RGGNCCY 2 cut(s) 260, 333
EcoRI GAATTC 3 cut(s) 1591, 1928, 2016
EcoRII CCWGG 4 cut(s) 188, 1932, 2291, 2766
EcoT14I CCWWGG 3 cut(s) 247, 1920, 2124
EcoT22I ATGCAT 2 cut(s) 1585, 1627
EcoT38I GRGCYC 6 cut(s) 150, 199, 297, 336, 1703, 2050
ErhI CCWWGG 3 cut(s) 247, 1920, 2124
FaqI GGGAC 5 cut(s) 211, 574, 1720, 2317, 2617
FauI CCCGC 4 cut(s) 623, 1025, 1744, 2643
FblI GTMKAC 2 cut(s) 411, 2788
FokI GGATG 5 cut(s) 1402, 1874, 1937, 2141, 2155
FriOI GRGCYC 6 cut(s) 150, 199, 297, 336, 1703, 2050
FspBI CTAG 8 cut(s) 65, 134, 1257, 1485, 1820, 2136, 2162, 2417
GlaI GCGC 5 cut(s) 85, 211, 516, 1014, 1681
GsaI CCCAGC 3 cut(s) 506, 645, 2595
GsuI CTGGAG 4 cut(s) 296, 458, 505, 2737
HaeII RGCGCY 3 cut(s) 87, 1016, 1683
HgaI GACGC 1 cut(s) 2744
HhaI GCGC 5 cut(s) 86, 212, 517, 1015, 1682
Hin6I GCGC 5 cut(s) 84, 210, 515, 1013, 1680
HinP1I GCGC 5 cut(s) 84, 210, 515, 1013, 1680
HincII GTYRAC 2 cut(s) 412, 2400
HindII GTYRAC 2 cut(s) 412, 2400
HindIII AAGCTT 1 cut(s) 2173
HpaI GTTAAC 1 cut(s) 2400
HphI GGTGA 6 cut(s) 1604, 1742, 2228, 2266, 2285, 2731
Hpy166II GTNNAC 4 cut(s) 412, 978, 2400, 2789
Hpy8I GTNNAC 4 cut(s) 412, 978, 2400, 2789
Hpy99I CGWCG 1 cut(s) 176
HpyCH4III ACNGT 8 cut(s) 598, 624, 1492, 1565, 1690, 1718, 1868, 1970
HpyCH4IV ACGT 2 cut(s) 201, 1788
HpySE526I ACGT 2 cut(s) 201, 1788
HspAI GCGC 5 cut(s) 84, 210, 515, 1013, 1680
KspAI GTTAAC 1 cut(s) 2400
KspI CCGCGG 1 cut(s) 1002
Kzo9I GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
LmnI GCTCC 7 cut(s) 369, 759, 1001, 1510, 1698, 2269, 2392
Lsp1109I GCAGC 6 cut(s) 7, 10, 748, 1403, 2440, 2485
LweI GCATC 5 cut(s) 1570, 1612, 1634, 2068, 2553
MaeI CTAG 8 cut(s) 65, 134, 1257, 1485, 1820, 2136, 2162, 2417
MaeII ACGT 2 cut(s) 201, 1788
MaeIII GTNAC 5 cut(s) 614, 1690, 1789, 2287, 2335
MalI GATC 5 cut(s) 819, 1080, 1284, 2190, 2281
MbiI CCGCTC 1 cut(s) 2095
MboI GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
MflI RGATCY 4 cut(s) 817, 1282, 2188, 2279
MhlI GDGCHC 7 cut(s) 150, 199, 297, 336, 1703, 2050, 2574
MlyI GAGTC 4 cut(s) 1180, 1742, 2744, 2825
MmeI TCCRAC 3 cut(s) 710, 1260, 2051
Mph1103I ATGCAT 2 cut(s) 1585, 1627
MroXI GAANNNNTTC 3 cut(s) 323, 1681, 2078
MslI CAYNNNNRTG 4 cut(s) 15, 327, 731, 1440
MspA1I CMGCKG 4 cut(s) 121, 502, 1001, 2831
MspR9I CCNGG 8 cut(s) 190, 984, 985, 1934, 2293, 2302, 2569, 2768
Mva1269I GAATGC 3 cut(s) 475, 967, 1583
MvaI CCWGG 4 cut(s) 190, 1934, 2293, 2768
MvnI CGCG 1 cut(s) 1001
NciI CCSGG 4 cut(s) 984, 985, 2302, 2569
NdeII GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
NmeAIII GCCGAG 1 cut(s) 491
NmuCI GTSAC 3 cut(s) 1789, 2287, 2335
NsiI ATGCAT 2 cut(s) 1585, 1627
NspI RCATGY 2 cut(s) 35, 586
NspV TTCGAA 4 cut(s) 420, 959, 1783, 2082
OliI CACNNNNGTG 3 cut(s) 15, 327, 731
PciI ACATGT 1 cut(s) 582
PcsI WCGNNNNNNNCGW 2 cut(s) 417, 426
PctI GAATGC 3 cut(s) 475, 967, 1583
PdmI GAANNNNTTC 3 cut(s) 323, 1681, 2078
PflFI GACNNNGTC 1 cut(s) 1968
PleI GAGTC 4 cut(s) 1179, 1741, 2744, 2824
PpsI GAGTC 4 cut(s) 1179, 1741, 2744, 2824
PpuMI RGGWCCY 1 cut(s) 260
PscI ACATGT 1 cut(s) 582
PsiI TTATAA 2 cut(s) 1136, 1848
Psp124BI GAGCTC 3 cut(s) 150, 297, 1703
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 4 cut(s) 188, 1932, 2291, 2766
PspFI CCCAGC 3 cut(s) 502, 641, 2591
PspGI CCWGG 4 cut(s) 188, 1932, 2291, 2766
PspOMI GGGCCC 2 cut(s) 195, 332
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 1244
PsuI RGATCY 4 cut(s) 817, 1282, 2188, 2279
PsyI GACNNNGTC 1 cut(s) 1968
PvuII CAGCTG 2 cut(s) 121, 502
RsaI GTAC 5 cut(s) 1165, 1447, 1562, 1870, 2315
RsaNI GTAC 5 cut(s) 1164, 1446, 1561, 1869, 2314
RseI CAYNNNNRTG 4 cut(s) 15, 327, 731, 1440
SacI GAGCTC 3 cut(s) 150, 297, 1703
SacII CCGCGG 1 cut(s) 1002
SalI GTCGAC 1 cut(s) 410
Sau3AI GATC 5 cut(s) 817, 1078, 1282, 2188, 2279
SchI GAGTC 4 cut(s) 1180, 1742, 2744, 2825
ScrFI CCNGG 8 cut(s) 190, 984, 985, 1934, 2293, 2302, 2569, 2768
SduI GDGCHC 7 cut(s) 150, 199, 297, 336, 1703, 2050, 2574
SfaNI GCATC 5 cut(s) 1570, 1612, 1634, 2068, 2553
SfcI CTRYAG 1 cut(s) 24
SfiI GGCCNNNNNGGCC 1 cut(s) 194
Sfr303I CCGCGG 1 cut(s) 1002
SfuI TTCGAA 4 cut(s) 420, 959, 1783, 2082
SgrBI CCGCGG 1 cut(s) 1002
SinI GGWCC 5 cut(s) 238, 260, 557, 707, 917
SmaI CCCGGG 1 cut(s) 985
SmiMI CAYNNNNRTG 4 cut(s) 15, 327, 731, 1440
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SspI AATATT 1 cut(s) 1300
SspMI CTAG 8 cut(s) 65, 134, 1257, 1485, 1820, 2136, 2162, 2417
SstI GAGCTC 3 cut(s) 150, 297, 1703
StyD4I CCNGG 8 cut(s) 188, 982, 983, 1932, 2291, 2300, 2567, 2766
StyI CCWWGG 3 cut(s) 247, 1920, 2124
TaaI ACNGT 8 cut(s) 598, 624, 1492, 1565, 1690, 1718, 1868, 1970
TaiI ACGT 2 cut(s) 204, 1791
TaqII GACCGA 1 cut(s) 545
TatI WGTACW 1 cut(s) 1560
TauI GCSGC 4 cut(s) 696, 1385, 2095, 2707
TscAI CASTG 5 cut(s) 295, 334, 1495, 2579, 2584
TseFI GTSAC 3 cut(s) 1789, 2287, 2335
TseI GCWGC 6 cut(s) 20, 23, 736, 1391, 2453, 2498
Tsp45I GTSAC 3 cut(s) 1789, 2287, 2335
TspDTI ATGAA 3 cut(s) 1941, 2167, 2541
TspGWI ACGGA 5 cut(s) 633, 1071, 1987, 2057, 2272
TspMI CCCGGG 1 cut(s) 983
TspRI CASTG 5 cut(s) 295, 334, 1495, 2579, 2584
Tth111I GACNNNGTC 1 cut(s) 1968
VpaK11BI GGWCC 5 cut(s) 238, 260, 557, 707, 917
XapI RAATTY 5 cut(s) 929, 1319, 1591, 1928, 2016
XbaI TCTAGA 1 cut(s) 133
XceI RCATGY 2 cut(s) 35, 586
XmaI CCCGGG 1 cut(s) 983
XmiI GTMKAC 2 cut(s) 411, 2788
XmnI GAANNNNTTC 3 cut(s) 323, 1681, 2078
XspI CTAG 8 cut(s) 65, 134, 1257, 1485, 1820, 2136, 2162, 2417
Zsp2I ATGCAT 2 cut(s) 1585, 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.