MD14G1038700.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003268
Physical Location & Seq
Reverse (-)
3522327 .. 3526412
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1038700.v1.1.491

Sequence Viewer

Length: 2877 bp
ATGGAAGAACACCATAGTGCTGCAGCGAAGCTTCCTCTCTCACTCCTCCTCCTCCTTCTCTCACTTGCTTCATTTGCTCTGAGCGCCACAGACCCGAACGACCTAGCAATCCTCAACCAGCTGAGAAAAAACCTACAAAACCCAGAGCTCCTGGAGTGGCCGGAAAATGGCGACGACCCGTGTGTGGCCAGTTGGCCCCACGTCTTCTGCGCCGGTTCGAGAGTGTCCCAGATTCAAGTCCAGAACCTTGGCCTGAAGGGTCCTCTGCCCCAGAACTTGAACCAGCTCACTGAGCTCTCAAACATTGGCCTCCAGAGGAACCAGTTCAGTGGGCCCATCCCTTCCCTCAAAGGGCTGTCGAAGCTCCGGTTTGCTTACTTGGATTTCAACAACTTCACTTCGATTCCTGTCGACTTCTTCGAAGGCCTCGATTCTCTTGAGGTTCTGGCGTTGGACGGCAACAATTTGAATGGCACGACAGGCTGGAATTTCCCTCCTCAGCTGGGGAACTCGGCGCAGTTGCAGAACCTTAGTTGCATGAGTTGTAATTTGATCGGTCCGTTGCCCGATTTTCTCGGGAACATGTCGTCCTTAACGGTTTTGCAACTGTCGGGCAACGGACTGTCCGGCGGCATCCCGCCGAGCTTAACGGGCCTTAATTTGCAGATTCTTTGGCTGAACAACCCCACCGGCGACGGATTGAGTGGTCCGATTGATGTTCTGACCACAATGGTGCAGCTCAACAGCGTGTGGCTTCACGGGAACCAGTTTTCCGGCGTGATTCCGGACAGCATTGGTAATTTAACTTCTTTGAAAGATCTCAATCTTAATCAAAACCAATTTGTTGGTTTAGTTCCTGATGGTTTGGCTAATTTGGCACTTGATAGATTGATCTTGAACAATAATCTTTTGATGGGTCCAATCCCGAAATTCAAAGCTCGCAATGCGAGTTTTGATACGAATGCGTTTTGTCAATCCACTCCTGGGGTTCCTTGCGCCGCGGAGGTTATGGCGCTCATCGAGTTCCTTGACGGGTTGAATTACCCTTCAACGCTTGTTTCTAAGTGGTCTGGTAATGATCCGTGCGGGTCGTGGTTGGGAGTGAGTTGTGGGAACAATGGGAAGGTGTCTGTTATAAATCTGCCCAAGTATAATCTGAATGGTACCTTGAGTCCTTCGGTTGCCAACTTAGAATCTCTTGTTCAAATTAGGCTTCAGAATAACAATTTGCAAGGTTTTGTTCCTGACAATTGGACTAGCTTGAAATCCTTGACTGAGTTGGATCTTAGTGGGAACAATATTTCCCCTCCATTGCCAAAATTCAGTAGCACCGTCAAAGTTTCCGTTGATGGGAATCGTTTGTTTAATGGTAATCCATCTGCGCCGGGTGCTACACCAAAGGGTAGCCCTTCTTCATCGACAGCACCAAAGGGTAGCCCTTCCTCATCGACAGGCTCAGGTTCTCGTGTCAATGGTACTTCTGAACCAAACCAACAAAAAGGATCGAAAAGGTCTAGCATTGTTTTTATTGTAGCTCCGATTGCAAGTGTTGCTGCTATTGCTGTTTTGCTTGTACTTCCTTTATCTATGTACTGCTGTAAGAAGAGAAGAGATGCAATTCAGAATTCAAGTTCACTTGTAATTCACCCGAGAGATCCATCTGATCCAGATAATATGGTTAAGGTTGTTGTTGCCGATAATACCCATGGAAGTGCTTCTACAGTTACAGGGAGCTCTGCAAGCAGAAACAGTAGTGGTAGAGCTGAGTCTCATGTCATTGAAGCTGGGAATCTCATCATATCAGTTCAAGTTCTTCGAAATGTGACAAAGAATTTCGCCCCAGAAAATGAGTTAGGCCGTGGTGGCTTTGGGGTGGTTTATAAGGGAGAATTGGATGACGGGACAAAAATAGCAGTGAAAAGAATGGAGGCTGGTGTGATTAGCAACAAAGCGTTGGATGAATTCCAGTCCGAAATTGCAGTTCTGTCAAAGGTCAGACACCGTCATTTGGTATCACTTTTGGGTTATTCTGTTGAAGGAAATGAGAGAATGCTCGTCTATGAATATATGCCTCAAGGTGCTCTGAGCAGGCATCTTTTCCATTGGAAGACCTTCGAATTAGAGCCGCTCTCTTGGAAGAGGAGGCTAAACATTGCCTTGGATGTTGCTAGGGGGATGGATTATCTTCACAATCTAGCTCACAAAAGCTTCATACACAGAGATCTTAAATCATCAAATATCTTACTTGGTGATGATTTTAAAGCAAAAGTTTCGGATTTTGGATTGGTGAAACTGGCTCCTGATGGTGAAAAATCCGTTGTGACCAGGCTTGCCGGGACTTTTGGTTACTTAGCACCAGAGTATGCTGTGACGGGAAAAATCACAACGAAAGTAGATGTCTTCAGTTTCGGGGTTGTGTTGATGGAGCTATTAACTGGAATGATGGCACTAGATGAGGATAGACCTGAAGAAAGCCAATACTTGGCTGCATGGTTCTGGCACATAAAATCGAATAAGGAGAAACTCATGGCTGCTATTGACCCAACTCTTGATAGAAAAGAAGAAACATTTGAGACCATCGCTATAATTGCAGAACTCGCTGGGCACTGCACTGCAAGGGAACCCAGCCAAAGACCGGATATGAGCCATGCCGTGAATGTATTGTCCCCGCTTGTTGAAAAATGGAAACCTGTCGATGATGAGAATGAGGAGTATTCTGGGATTGATTATAGCCAGCCGCTTAACCAGATGGTGAAGGGTTGGCAGGACGCAGAAGGAAAGGACTCCGGTTATCTGGACCTGGAAGACAGCAAGGGGAGTATACCGGCAAGGCCAACTGGCTTTGCAGAGTCTTTTACTTCCGCTGATGGTCGGTAA

Protein Analysis

959

Amino Acids

103.52

Weight (kDa)

5.62

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 334 - 370 4.1e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 394 - 434 9.4e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 615 - 887 2.9e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 615 - 886 4.5e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1136, 1881
Acc65I GGTACC 1 cut(s) 1163
AccB1I GGYRCC 1 cut(s) 1163
AccB7I CCANNNNNTGG 1 cut(s) 2482
AccBSI CCGCTC 1 cut(s) 2128
AccI GTMKAC 2 cut(s) 411, 2821
AccII CGCG 1 cut(s) 1001
AccIII TCCGGA 1 cut(s) 784
AciI CCGC 9 cut(s) 630, 638, 999, 1001, 1086, 2126, 2669, 2738, 2862
AclWI GGATC 5 cut(s) 1073, 1290, 1510, 1649, 1658
AcoI YGGCCR 2 cut(s) 158, 186
AcsI RAATTY 6 cut(s) 487, 929, 1319, 1624, 1831, 1961
AcuI CTGAAG 4 cut(s) 275, 1199, 2386, 2487
AfaI GTAC 4 cut(s) 1165, 1477, 1575, 1592
AfiI CCNNNNNNNGG 9 cut(s) 167, 184, 351, 503, 984, 1350, 2008, 2482, 2635
AflIII ACRYGT 1 cut(s) 582
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 4 cut(s) 150, 982, 2324, 2799
AleI CACNNNNGTG 2 cut(s) 15, 731
AloI GAACNNNNNNTCC 6 cut(s) 572, 604, 755, 787, 1286, 1318
Alw21I GWGCWC 4 cut(s) 150, 297, 1736, 2083
Alw26I GTCTC 2 cut(s) 1773, 2567
AlwI GGATC 5 cut(s) 1073, 1290, 1510, 1649, 1658
Ama87I CYCGRG 2 cut(s) 575, 1648
Aor13HI TCCGGA 1 cut(s) 784
ApaI GGGCCC 1 cut(s) 336
ApeKI GCWGC 6 cut(s) 20, 23, 736, 1553, 2486, 2531
ApoI RAATTY 6 cut(s) 487, 929, 1319, 1624, 1831, 1961
Asp700I GAANNNNTTC 3 cut(s) 323, 1714, 2111
Asp718I GGTACC 1 cut(s) 1163
AspLEI GCGC 6 cut(s) 86, 212, 517, 998, 1015, 1384
AspS9I GGNCC 9 cut(s) 195, 260, 332, 333, 557, 652, 707, 917, 2797
AsuC2I CCSGG 2 cut(s) 1386, 2335
AsuHPI GGTGA 5 cut(s) 1637, 2261, 2299, 2318, 2764
AsuII TTCGAA 3 cut(s) 420, 1816, 2115
AvaI CYCGRG 2 cut(s) 575, 1648
AvaII GGWCC 5 cut(s) 260, 557, 707, 917, 2797
BaeGI GKGCMC 2 cut(s) 336, 2607
BalI TGGCCA 1 cut(s) 188
BanI GGYRCC 1 cut(s) 1163
BanII GRGCYC 4 cut(s) 150, 297, 336, 1736
BarI GAAGNNNNNNTAC 6 cut(s) 790, 822, 1396, 1428, 1702, 1734
BauI CACGAG 1 cut(s) 1464
BbsI GAAGAC 4 cut(s) 196, 2114, 2392, 2811
Bbv12I GWGCWC 4 cut(s) 150, 297, 1736, 2083
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 6 cut(s) 7, 35, 748, 1540, 2473, 2518
BceAI ACGGC 3 cut(s) 472, 1842, 2636
BcgI CGANNNNNNTGC 2 cut(s) 2253, 2287
BciT130I CCWGG 4 cut(s) 152, 984, 2326, 2801
BcnI CCSGG 2 cut(s) 1386, 2335
BcoDI GTCTC 2 cut(s) 1773, 2567
BfaI CTAG 6 cut(s) 104, 1257, 1515, 2169, 2195, 2450
BfmI CTRYAG 2 cut(s) 21, 1719
BfoI RGCGCY 2 cut(s) 87, 1016
BglI GCCNNNNNGGC 1 cut(s) 1863
BglII AGATCT 2 cut(s) 817, 2221
Bme1390I CCNGG 6 cut(s) 152, 984, 1386, 2326, 2335, 2801
Bme18I GGWCC 5 cut(s) 260, 557, 707, 917, 2797
BmeT110I CYCGRG 2 cut(s) 575, 1648
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 9 cut(s) 195, 260, 332, 333, 557, 652, 707, 917, 2797
BmrFI CCNGG 6 cut(s) 152, 984, 1386, 2326, 2335, 2801
BmsI GCATC 3 cut(s) 642, 1603, 2101
BpiI GAAGAC 4 cut(s) 196, 2114, 2392, 2811
BplI GAGNNNNNCTC 2 cut(s) 2114, 2146
BpmI CTGGAG 2 cut(s) 173, 296
Bpu10I CCTNAGC 2 cut(s) 498, 1456
Bpu14I TTCGAA 3 cut(s) 420, 1816, 2115
BpuEI CTTGAG 3 cut(s) 458, 1189, 2058
BpuMI CCSGG 2 cut(s) 1386, 2335
BsaBI GATNNNNATC 2 cut(s) 822, 1353
BsaI GGTCTC 1 cut(s) 2567
BsaJI CCNNGG 6 cut(s) 247, 983, 999, 1705, 1858, 2157
BsaWI WCCGGW 4 cut(s) 366, 784, 2635, 2786
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 9 cut(s) 167, 184, 351, 503, 984, 1350, 2008, 2482, 2635
Bse118I RCCGGY 3 cut(s) 212, 689, 2824
Bse1I ACTGG 7 cut(s) 189, 322, 766, 1966, 2298, 2440, 2842
Bse3DI GCAATG 3 cut(s) 949, 1310, 2151
Bse8I GATNNNNATC 2 cut(s) 822, 1353
BseAI TCCGGA 1 cut(s) 784
BseBI CCWGG 4 cut(s) 152, 984, 2326, 2801
BseDI CCNNGG 6 cut(s) 247, 983, 999, 1705, 1858, 2157
BseGI GGATG 6 cut(s) 336, 633, 1900, 1963, 2167, 2181
BseJI GATNNNNATC 2 cut(s) 822, 1353
BseLI CCNNNNNNNGG 9 cut(s) 167, 184, 351, 503, 984, 1350, 2008, 2482, 2635
BseMI GCAATG 3 cut(s) 949, 1310, 2151
BseMII CTCAG 8 cut(s) 71, 113, 282, 512, 1266, 1470, 1755, 2075
BseNI ACTGG 7 cut(s) 189, 322, 766, 1966, 2298, 2440, 2842
BseRI GAGGAG 6 cut(s) 35, 38, 41, 486, 2155, 2723
BseSI GKGCMC 2 cut(s) 336, 2607
BseXI GCAGC 6 cut(s) 7, 35, 748, 1540, 2473, 2518
BseYI CCCAGC 4 cut(s) 502, 1784, 2600, 2624
BsgI GTGCAG 2 cut(s) 755, 2593
Bsh1236I CGCG 1 cut(s) 1001
BshNI GGYRCC 1 cut(s) 1163
BsiHKAI GWGCWC 4 cut(s) 150, 297, 1736, 2083
BsiHKCI CYCGRG 2 cut(s) 575, 1648
BslFI GGGAC 4 cut(s) 211, 1915, 2350, 2650
BslI CCNNNNNNNGG 9 cut(s) 167, 184, 351, 503, 984, 1350, 2008, 2482, 2635
BsmAI GTCTC 2 cut(s) 1773, 2567
BsmFI GGGAC 4 cut(s) 211, 1915, 2350, 2650
BsmI GAATGC 2 cut(s) 967, 2055
Bso31I GGTCTC 1 cut(s) 2567
BsoBI CYCGRG 2 cut(s) 575, 1648
Bsp119I TTCGAA 3 cut(s) 420, 1816, 2115
Bsp120I GGGCCC 1 cut(s) 332
Bsp1286I GDGCHC 6 cut(s) 150, 297, 336, 1736, 2083, 2607
Bsp13I TCCGGA 1 cut(s) 784
Bsp143I GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
Bsp19I CCATGG 1 cut(s) 1705
BspACI CCGC 9 cut(s) 630, 638, 999, 1001, 1086, 2126, 2669, 2738, 2862
BspCNI CTCAG 8 cut(s) 72, 114, 283, 511, 1267, 1469, 1756, 2076
BspEI TCCGGA 1 cut(s) 784
BspFNI CGCG 1 cut(s) 1001
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 5 cut(s) 1073, 1290, 1510, 1649, 1658
BspT104I TTCGAA 3 cut(s) 420, 1816, 2115
BspT107I GGYRCC 1 cut(s) 1163
BspTNI GGTCTC 1 cut(s) 2567
BsrBI CCGCTC 1 cut(s) 2128
BsrDI GCAATG 3 cut(s) 949, 1310, 2151
BsrFI RCCGGY 3 cut(s) 212, 689, 2824
BsrI ACTGG 7 cut(s) 189, 322, 766, 1966, 2298, 2440, 2842
BssAI RCCGGY 3 cut(s) 212, 689, 2824
BssECI CCNNGG 6 cut(s) 247, 983, 999, 1705, 1858, 2157
BssMI GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
BssNAI GTATAC 1 cut(s) 2822
BssSI CACGAG 1 cut(s) 1464
BssT1I CCWWGG 3 cut(s) 247, 1705, 2157
Bst1107I GTATAC 1 cut(s) 2822
Bst2BI CACGAG 1 cut(s) 1464
Bst2UI CCWGG 4 cut(s) 152, 984, 2326, 2801
Bst4CI ACNGT 7 cut(s) 598, 609, 624, 1333, 1723, 1751, 2003
Bst6I CTCTTC 3 cut(s) 1598, 1603, 2132
BstAPI GCANNNNNTGC 1 cut(s) 1550
BstBI TTCGAA 3 cut(s) 420, 1816, 2115
BstC8I GCNNGC 5 cut(s) 940, 1741, 2090, 2331, 2735
BstDSI CCRYGG 3 cut(s) 999, 1705, 1858
BstF5I GGATG 6 cut(s) 336, 633, 1900, 1963, 2167, 2181
BstFNI CGCG 1 cut(s) 1001
BstH2I RGCGCY 2 cut(s) 87, 1016
BstHHI GCGC 6 cut(s) 86, 212, 517, 998, 1015, 1384
BstKTI GATC 9 cut(s) 555, 820, 894, 1081, 1285, 1505, 1657, 1666, 2224
BstMAI GTCTC 2 cut(s) 1773, 2567
BstMBI GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
BstNI CCWGG 4 cut(s) 152, 984, 2326, 2801
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 6 cut(s) 150, 982, 1384, 2324, 2333, 2799
BstSFI CTRYAG 2 cut(s) 21, 1719
BstSLI GKGCMC 2 cut(s) 336, 2607
BstUI CGCG 1 cut(s) 1001
BstV1I GCAGC 6 cut(s) 7, 35, 748, 1540, 2473, 2518
BstV2I GAAGAC 4 cut(s) 196, 2114, 2392, 2811
BstX2I RGATCY 4 cut(s) 817, 1282, 1654, 2221
BstXI CCANNNNNNTGG 3 cut(s) 248, 329, 845
BstYI RGATCY 4 cut(s) 817, 1282, 1654, 2221
BstZ17I GTATAC 1 cut(s) 2822
BtgI CCRYGG 3 cut(s) 999, 1705, 1858
BtgZI GCGATG 1 cut(s) 2563
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 6 cut(s) 336, 633, 1900, 1963, 2167, 2181
BtsI GCAGTG 3 cut(s) 1920, 2605, 2610
BtsIMutI CAGTG 5 cut(s) 288, 334, 1920, 2605, 2610
Cac8I GCNNGC 5 cut(s) 940, 1741, 2090, 2331, 2735
CfoI GCGC 6 cut(s) 86, 212, 517, 998, 1015, 1384
Cfr10I RCCGGY 3 cut(s) 212, 689, 2824
Cfr13I GGNCC 9 cut(s) 195, 260, 332, 333, 557, 652, 707, 917, 2797
Cfr42I CCGCGG 1 cut(s) 1002
CpoI CGGWCCG 1 cut(s) 557
CseI GACGC 1 cut(s) 2777
Csp6I GTAC 4 cut(s) 1164, 1476, 1574, 1591
CspI CGGWCCG 1 cut(s) 557
CviAII CATG 7 cut(s) 538, 583, 1706, 1772, 2490, 2527, 2648
CviQI GTAC 4 cut(s) 1164, 1476, 1574, 1591
DpnI GATC 9 cut(s) 554, 819, 893, 1080, 1284, 1504, 1656, 1665, 2223
DpnII GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
DraI TTTAAA 1 cut(s) 2260
EaeI YGGCCR 2 cut(s) 158, 186
Eam1104I CTCTTC 3 cut(s) 1598, 1603, 2132
EarI CTCTTC 3 cut(s) 1598, 1603, 2132
Ecl136II GAGCTC 3 cut(s) 148, 295, 1734
Eco130I CCWWGG 3 cut(s) 247, 1705, 2157
Eco147I AGGCCT 1 cut(s) 426
Eco24I GRGCYC 4 cut(s) 150, 297, 336, 1736
Eco31I GGTCTC 1 cut(s) 2567
Eco47I GGWCC 5 cut(s) 260, 557, 707, 917, 2797
Eco53kI GAGCTC 3 cut(s) 148, 295, 1734
Eco57I CTGAAG 4 cut(s) 275, 1199, 2386, 2487
Eco88I CYCGRG 2 cut(s) 575, 1648
EcoICRI GAGCTC 3 cut(s) 148, 295, 1734
EcoO109I RGGNCCY 1 cut(s) 260
EcoRI GAATTC 2 cut(s) 1624, 1961
EcoRII CCWGG 4 cut(s) 150, 982, 2324, 2799
EcoT14I CCWWGG 3 cut(s) 247, 1705, 2157
EcoT38I GRGCYC 4 cut(s) 150, 297, 336, 1736
ErhI CCWWGG 3 cut(s) 247, 1705, 2157
FaeI CATG 7 cut(s) 541, 586, 1709, 1775, 2493, 2530, 2651
FaqI GGGAC 4 cut(s) 211, 1915, 2350, 2650
FatI CATG 7 cut(s) 537, 582, 1705, 1771, 2489, 2526, 2647
FauI CCCGC 3 cut(s) 645, 1079, 2676
FblI GTMKAC 2 cut(s) 411, 2821
FokI GGATG 6 cut(s) 323, 620, 1907, 1970, 2174, 2188
FriOI GRGCYC 4 cut(s) 150, 297, 336, 1736
FspBI CTAG 6 cut(s) 104, 1257, 1515, 2169, 2195, 2450
GlaI GCGC 6 cut(s) 85, 211, 516, 997, 1014, 1383
GsaI CCCAGC 4 cut(s) 506, 1788, 2604, 2628
GsuI CTGGAG 2 cut(s) 173, 296
HaeII RGCGCY 2 cut(s) 87, 1016
HgaI GACGC 1 cut(s) 2777
HhaI GCGC 6 cut(s) 86, 212, 517, 998, 1015, 1384
Hin1II CATG 7 cut(s) 541, 586, 1709, 1775, 2493, 2530, 2651
Hin6I GCGC 6 cut(s) 84, 210, 515, 996, 1013, 1382
HinP1I GCGC 6 cut(s) 84, 210, 515, 996, 1013, 1382
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HindIII AAGCTT 2 cut(s) 29, 2206
HphI GGTGA 5 cut(s) 1637, 2261, 2299, 2318, 2764
Hpy166II GTNNAC 3 cut(s) 412, 1634, 2822
Hpy8I GTNNAC 3 cut(s) 412, 1634, 2822
Hpy99I CGWCG 2 cut(s) 176, 698
HpyCH4III ACNGT 7 cut(s) 598, 609, 624, 1333, 1723, 1751, 2003
HpyCH4IV ACGT 1 cut(s) 201
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 7 cut(s) 541, 586, 1709, 1775, 2493, 2530, 2651
HspAI GCGC 6 cut(s) 84, 210, 515, 996, 1013, 1382
Kpn2I TCCGGA 1 cut(s) 784
KpnI GGTACC 1 cut(s) 1167
KspI CCGCGG 1 cut(s) 1002
Kzo9I GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
LmnI GCTCC 6 cut(s) 153, 369, 1540, 1731, 2302, 2425
Lsp1109I GCAGC 6 cut(s) 7, 35, 748, 1540, 2473, 2518
LweI GCATC 3 cut(s) 642, 1603, 2101
MaeI CTAG 6 cut(s) 104, 1257, 1515, 2169, 2195, 2450
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 5 cut(s) 1723, 1822, 2320, 2345, 2368
MalI GATC 9 cut(s) 554, 819, 893, 1080, 1284, 1504, 1656, 1665, 2223
MbiI CCGCTC 1 cut(s) 2128
MboI GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
MfeI CAATTG 1 cut(s) 1249
MflI RGATCY 4 cut(s) 817, 1282, 1654, 2221
MhlI GDGCHC 6 cut(s) 150, 297, 336, 1736, 2083, 2607
MlsI TGGCCA 1 cut(s) 188
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 4 cut(s) 1180, 1775, 2777, 2858
MmeI TCCRAC 3 cut(s) 432, 1260, 1935
Mox20I TGGCCA 1 cut(s) 188
MroI TCCGGA 1 cut(s) 784
MroXI GAANNNNTTC 3 cut(s) 323, 1714, 2111
MscI TGGCCA 1 cut(s) 188
MslI CAYNNNNRTG 3 cut(s) 15, 731, 1710
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 4 cut(s) 121, 502, 1001, 2864
MspR9I CCNGG 6 cut(s) 152, 984, 1386, 2326, 2335, 2801
MunI CAATTG 1 cut(s) 1249
Mva1269I GAATGC 2 cut(s) 967, 2055
MvaI CCWGG 4 cut(s) 152, 984, 2326, 2801
MvnI CGCG 1 cut(s) 1001
NciI CCSGG 2 cut(s) 1386, 2335
NcoI CCATGG 1 cut(s) 1705
NdeII GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
NlaIII CATG 7 cut(s) 541, 586, 1709, 1775, 2493, 2530, 2651
NmeAIII GCCGAG 2 cut(s) 491, 666
NmuCI GTSAC 3 cut(s) 1822, 2320, 2368
NspI RCATGY 1 cut(s) 586
NspV TTCGAA 3 cut(s) 420, 1816, 2115
OliI CACNNNNGTG 2 cut(s) 15, 731
PceI AGGCCT 1 cut(s) 426
PciI ACATGT 1 cut(s) 582
PcsI WCGNNNNNNNCGW 2 cut(s) 417, 426
PctI GAATGC 2 cut(s) 967, 2055
PdmI GAANNNNTTC 3 cut(s) 323, 1714, 2111
PfeI GAWTC 8 cut(s) 232, 403, 431, 667, 781, 1193, 1354, 1789
PflFI GACNNNGTC 1 cut(s) 2001
PflMI CCANNNNNTGG 1 cut(s) 2482
PfoI TCCNGGA 1 cut(s) 150
PleI GAGTC 4 cut(s) 1179, 1774, 2777, 2857
PpsI GAGTC 4 cut(s) 1179, 1774, 2777, 2857
PpuMI RGGWCCY 1 cut(s) 260
PscI ACATGT 1 cut(s) 582
PsiI TTATAA 2 cut(s) 1136, 1881
Psp124BI GAGCTC 3 cut(s) 150, 297, 1736
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 4 cut(s) 150, 982, 2324, 2799
PspFI CCCAGC 4 cut(s) 502, 1784, 2600, 2624
PspGI CCWGG 4 cut(s) 150, 982, 2324, 2799
PspOMI GGGCCC 1 cut(s) 332
PspPI GGNCC 9 cut(s) 195, 260, 332, 333, 557, 652, 707, 917, 2797
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 1 cut(s) 25
PsuI RGATCY 4 cut(s) 817, 1282, 1654, 2221
PsyI GACNNNGTC 1 cut(s) 2001
PvuII CAGCTG 2 cut(s) 121, 502
RsaI GTAC 4 cut(s) 1165, 1477, 1575, 1592
RsaNI GTAC 4 cut(s) 1164, 1476, 1574, 1591
RseI CAYNNNNRTG 3 cut(s) 15, 731, 1710
Rsr2I CGGWCCG 1 cut(s) 557
RsrII CGGWCCG 1 cut(s) 557
SacI GAGCTC 3 cut(s) 150, 297, 1736
SacII CCGCGG 1 cut(s) 1002
SalI GTCGAC 1 cut(s) 410
Sau3AI GATC 9 cut(s) 552, 817, 891, 1078, 1282, 1502, 1654, 1663, 2221
Sau96I GGNCC 9 cut(s) 195, 260, 332, 333, 557, 652, 707, 917, 2797
SchI GAGTC 4 cut(s) 1180, 1775, 2777, 2858
ScrFI CCNGG 6 cut(s) 152, 984, 1386, 2326, 2335, 2801
SduI GDGCHC 6 cut(s) 150, 297, 336, 1736, 2083, 2607
SfaNI GCATC 3 cut(s) 642, 1603, 2101
SfcI CTRYAG 2 cut(s) 21, 1719
Sfr303I CCGCGG 1 cut(s) 1002
SfuI TTCGAA 3 cut(s) 420, 1816, 2115
SgrAI CRCCGGYG 1 cut(s) 689
SgrBI CCGCGG 1 cut(s) 1002
SinI GGWCC 5 cut(s) 260, 557, 707, 917, 2797
SmiMI CAYNNNNRTG 3 cut(s) 15, 731, 1710
SmlI CTYRAG 3 cut(s) 437, 1168, 2073
SmoI CTYRAG 3 cut(s) 437, 1168, 2073
SseBI AGGCCT 1 cut(s) 426
SsiI CCGC 9 cut(s) 630, 638, 999, 1001, 1086, 2126, 2669, 2738, 2862
SspI AATATT 1 cut(s) 1300
SspMI CTAG 6 cut(s) 104, 1257, 1515, 2169, 2195, 2450
SstI GAGCTC 3 cut(s) 150, 297, 1736
StuI AGGCCT 1 cut(s) 426
StyD4I CCNGG 6 cut(s) 150, 982, 1384, 2324, 2333, 2799
StyI CCWWGG 3 cut(s) 247, 1705, 2157
TaaI ACNGT 7 cut(s) 598, 609, 624, 1333, 1723, 1751, 2003
TaiI ACGT 1 cut(s) 204
TaqII GACCGA 1 cut(s) 545
TatI WGTACW 2 cut(s) 1573, 1590
TauI GCSGC 4 cut(s) 633, 1001, 2128, 2740
TfiI GAWTC 8 cut(s) 232, 403, 431, 667, 781, 1193, 1354, 1789
TscAI CASTG 5 cut(s) 295, 334, 1920, 2612, 2617
TseFI GTSAC 3 cut(s) 1822, 2320, 2368
TseI GCWGC 6 cut(s) 20, 23, 736, 1553, 2486, 2531
Tsp45I GTSAC 3 cut(s) 1822, 2320, 2368
TspDTI ATGAA 5 cut(s) 60, 1404, 1974, 2076, 2200
TspGWI ACGGA 6 cut(s) 549, 633, 711, 1071, 1333, 2305
TspRI CASTG 5 cut(s) 295, 334, 1920, 2612, 2617
Tth111I GACNNNGTC 1 cut(s) 2001
Van91I CCANNNNNTGG 1 cut(s) 2482
VpaK11BI GGWCC 5 cut(s) 260, 557, 707, 917, 2797
XapI RAATTY 6 cut(s) 487, 929, 1319, 1624, 1831, 1961
XceI RCATGY 1 cut(s) 586
XmiI GTMKAC 2 cut(s) 411, 2821
XmnI GAANNNNTTC 3 cut(s) 323, 1714, 2111
XspI CTAG 6 cut(s) 104, 1257, 1515, 2169, 2195, 2450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.