FvH4_3g28170

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007924
Physical Location & Seq
Forward (+)
21151266 .. 21157182
5917 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28170.t2

Sequence Viewer

Length: 1029 bp
ATGGTGTCGTCGTCTTCGGCTCTGTCAAGGCTGTCCAGTATCAGCCGACACTTCAATCCCTCCCACGCAAGCAAGGATGATGATGTTGTGGCCTCCATCAAGATTTCTTTAGAGGTCTCCGAAGCTTTGTCAAATGGGCGTGCCGTCGTTGCTCTCGAGTCCACCATCATCTCCCACGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCCGTAGTGAGGGAAAATGGGGCTGTTCCTGCCACCATTGCTATCTTGGATGGCACACCTTGCGTAGGTCTAACTATAAAAGAACTGGAGAAGCTTGCCAAACTGGGTCCAAGAGCTCAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGGCAACCAGAGGGAACGGTGCGACTACTGTTTCTGCAACAATGATTTTTGCTCATATGGTCGGCATCCCATTGTTTGTGACTGGGGGAATTGGAGGAGTACATCAACATGGCGAGAATACCATGGACATATCTTCTGATCTGACTGAGCTTGGAAGGACACCTGTTGCTGTCATCTCTGCTGGTGTAAAATCAATTTTGGATATTCCAAGGACCCTTGAATATTTGGAAACACAGGGAGTTTGTGTTGCTGCTTACAAGACCAATGAATTTCCAGCATTTTTCACAGAAGCAAGTGGATGCAAGGTGCCTTGCCGTGTTGATACCCTAGATGATTGTGCTCGGCTTATAGATGCAAACCTAAAACTTCAGCTTGGAACTGGAATGTTGATTGCTGTTCCAATTCCTAAAGAACACTCGGCTTCGGGAAGTTTAATCGAGTCTGCAATACAGACAGCCCTTAGAGAAGCTAGGGATAAGAACATAACAGGCAATGCTGCAACTCCATTCTTGCTTGCAAGAGTAAATGAACTAACTGGAGGAGCATCTTTGACTTCAAACATTGCGCTAGTGAAGAATAATGCACTTGTTGGTGCTAAAATCGCTGTAGCACTTTCCCAGATCAGAGAATGTTGTAGAAAAGGTGGTTTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

343

Amino Acids

35.89

Weight (kDa)

7.08

Isoelectric Point (pI)

21.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 38 - 312 1.5e-123 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 679
AcsI RAATTY 2 cut(s) 196, 641
AcuI CTGAAG 1 cut(s) 725
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 2 cut(s) 231, 287
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 3 cut(s) 55, 593, 930
AluBI AGCT 7 cut(s) 125, 316, 338, 350, 523, 745, 842
AluI AGCT 7 cut(s) 125, 316, 338, 350, 523, 745, 842
Alw21I GWGCWC 2 cut(s) 340, 715
Alw26I GTCTC 3 cut(s) 121, 197, 351
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 2 cut(s) 90, 222
ApeKI GCWGC 2 cut(s) 623, 869
ApoI RAATTY 2 cut(s) 196, 641
AspLEI GCGC 1 cut(s) 940
AspS9I GGNCC 2 cut(s) 329, 585
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 2 cut(s) 329, 585
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 6, 350
Bbv12I GWGCWC 2 cut(s) 340, 715
BbvI GCAGC 2 cut(s) 610, 856
BccI CCATC 3 cut(s) 104, 173, 266
BceAI ACGGC 4 cut(s) 128, 169, 209, 672
BciVI GTATCC 1 cut(s) 198
BcoDI GTCTC 3 cut(s) 121, 197, 351
BfaI CTAG 3 cut(s) 701, 843, 941
BfmI CTRYAG 1 cut(s) 978
BfuI GTATCC 1 cut(s) 198
BisI GCNGC 2 cut(s) 624, 870
BlsI GCNGC 2 cut(s) 625, 871
Bme18I GGWCC 2 cut(s) 329, 585
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 2 cut(s) 329, 585
BmiI GGNNCC 3 cut(s) 330, 587, 681
BmrI ACTGGG 2 cut(s) 335, 465
BmsI GCATC 5 cut(s) 171, 447, 662, 715, 926
BmuI ACTGGG 2 cut(s) 335, 465
BpiI GAAGAC 2 cut(s) 6, 350
BpmI CTGGAG 2 cut(s) 329, 930
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 3 cut(s) 175, 495, 581
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 287
Bse1I ACTGG 6 cut(s) 36, 312, 330, 460, 757, 913
Bse3DI GCAATG 4 cut(s) 258, 360, 871, 933
BseDI CCNNGG 3 cut(s) 175, 495, 581
BseGI GGATG 5 cut(s) 82, 186, 277, 438, 677
BseLI CCNNNNNNNGG 2 cut(s) 231, 287
BseMI GCAATG 4 cut(s) 258, 360, 871, 933
BseMII CTCAG 2 cut(s) 353, 510
BseNI ACTGG 6 cut(s) 36, 312, 330, 460, 757, 913
BseRI GAGGAG 2 cut(s) 483, 927
BseXI GCAGC 2 cut(s) 610, 856
BshFI GGCC 2 cut(s) 92, 224
BshNI GGYRCC 1 cut(s) 679
BsiHKAI GWGCWC 2 cut(s) 340, 715
BsiHKCI CYCGRG 1 cut(s) 155
BslI CCNNNNNNNGG 2 cut(s) 231, 287
BsmAI GTCTC 3 cut(s) 121, 197, 351
BsnI GGCC 2 cut(s) 92, 224
Bso31I GGTCTC 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 340, 715
Bsp143I GATC 2 cut(s) 511, 993
Bsp19I CCATGG 1 cut(s) 495
BspANI GGCC 2 cut(s) 92, 224
BspCNI CTCAG 2 cut(s) 352, 511
BspLI GGNNCC 3 cut(s) 330, 587, 681
BspT107I GGYRCC 1 cut(s) 679
BspTNI GGTCTC 1 cut(s) 121
BsrDI GCAATG 4 cut(s) 258, 360, 871, 933
BsrI ACTGG 6 cut(s) 36, 312, 330, 460, 757, 913
BssECI CCNNGG 3 cut(s) 175, 495, 581
BssMI GATC 2 cut(s) 511, 993
BssT1I CCWWGG 2 cut(s) 495, 581
Bst4CI ACNGT 2 cut(s) 392, 403
Bst6I CTCTTC 1 cut(s) 349
BstAPI GCANNNNNTGC 1 cut(s) 282
BstC8I GCNNGC 4 cut(s) 70, 141, 318, 888
BstDEI CTNAG 3 cut(s) 339, 519, 833
BstDSI CCRYGG 2 cut(s) 175, 495
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 5 cut(s) 82, 186, 277, 438, 677
BstHHI GCGC 1 cut(s) 940
BstKTI GATC 2 cut(s) 514, 996
BstMAI GTCTC 3 cut(s) 121, 197, 351
BstMBI GATC 2 cut(s) 511, 993
BstMWI GCNNNNNNNGC 5 cut(s) 149, 251, 260, 282, 974
BstNSI RCATGY 1 cut(s) 371
BstSFI CTRYAG 1 cut(s) 978
BstV1I GCAGC 2 cut(s) 610, 856
BstV2I GAAGAC 2 cut(s) 6, 350
BstXI CCANNNNNNTGG 1 cut(s) 199
BsuI GTATCC 1 cut(s) 198
BsuRI GGCC 2 cut(s) 92, 224
BtgI CCRYGG 2 cut(s) 175, 495
BtsCI GGATG 5 cut(s) 82, 186, 277, 438, 677
Cac8I GCNNGC 4 cut(s) 70, 141, 318, 888
CfoI GCGC 1 cut(s) 940
Cfr13I GGNCC 2 cut(s) 329, 585
Csp6I GTAC 1 cut(s) 473
CviAII CATG 3 cut(s) 368, 482, 496
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 3 cut(s) 339, 519, 833
DpnI GATC 2 cut(s) 513, 995
DpnII GATC 2 cut(s) 511, 993
Eam1104I CTCTTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 349
Ecl136II GAGCTC 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 495, 581
Eco24I GRGCYC 1 cut(s) 340
Eco31I GGTCTC 1 cut(s) 121
Eco47I GGWCC 2 cut(s) 329, 585
Eco53kI GAGCTC 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 725
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 338
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 585
EcoT14I CCWWGG 2 cut(s) 495, 581
EcoT38I GRGCYC 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 495, 581
FaeI CATG 3 cut(s) 371, 485, 499
FaiI YATR 9 cut(s) 299, 369, 429, 431, 483, 497, 503, 722, 857
FatI CATG 3 cut(s) 367, 481, 495
FauNDI CATATG 1 cut(s) 429
Fnu4HI GCNGC 2 cut(s) 624, 870
FokI GGATG 5 cut(s) 89, 193, 284, 425, 684
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 2 cut(s) 624, 870
FspBI CTAG 3 cut(s) 701, 843, 941
GlaI GCGC 1 cut(s) 939
GluI GCNGC 2 cut(s) 624, 870
GsuI CTGGAG 2 cut(s) 329, 930
HaeIII GGCC 2 cut(s) 92, 224
HhaI GCGC 1 cut(s) 940
Hin1II CATG 3 cut(s) 371, 485, 499
Hin6I GCGC 1 cut(s) 938
HinP1I GCGC 1 cut(s) 938
HindIII AAGCTT 2 cut(s) 123, 314
HinfI GANTC 2 cut(s) 158, 812
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 5 cut(s) 121, 342, 511, 516, 998
Hpy188III TCNNGA 3 cut(s) 100, 155, 798
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 2 cut(s) 13, 149
HpyAV CCTTC 1 cut(s) 522
HpyCH4III ACNGT 2 cut(s) 392, 403
HpyCH4V TGCA 9 cut(s) 209, 365, 410, 675, 728, 818, 872, 890, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 251, 260, 282, 974
HpyF3I CTNAG 3 cut(s) 339, 519, 833
Hsp92II CATG 3 cut(s) 371, 485, 499
HspAI GCGC 1 cut(s) 938
Kzo9I GATC 2 cut(s) 511, 993
LmnI GCTCC 1 cut(s) 914
Lsp1109I GCAGC 2 cut(s) 610, 856
LweI GCATC 5 cut(s) 171, 447, 662, 715, 926
MaeI CTAG 3 cut(s) 701, 843, 941
MaeIII GTNAC 1 cut(s) 451
MalI GATC 2 cut(s) 513, 995
MboI GATC 2 cut(s) 511, 993
MboII GAAGA 5 cut(s) 6, 355, 366, 498, 958
MhlI GDGCHC 2 cut(s) 340, 715
MluCI AATT 5 cut(s) 196, 462, 567, 641, 774
MlyI GAGTC 2 cut(s) 167, 821
MnlI CCTC 9 cut(s) 70, 103, 106, 208, 214, 225, 377, 461, 905
MseI TTAA 1 cut(s) 806
MslI CAYNNNNRTG 1 cut(s) 480
MwoI GCNNNNNNNGC 5 cut(s) 149, 251, 260, 282, 974
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 429
NdeII GATC 2 cut(s) 511, 993
NlaIII CATG 3 cut(s) 371, 485, 499
NlaIV GGNNCC 3 cut(s) 330, 587, 681
NmeAIII GCCGAG 2 cut(s) 694, 770
NmuCI GTSAC 1 cut(s) 451
NspI RCATGY 1 cut(s) 371
PaeR7I CTCGAG 1 cut(s) 155
PciI ACATGT 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PkrI GCNGC 2 cut(s) 625, 871
PleI GAGTC 2 cut(s) 166, 820
PpsI GAGTC 2 cut(s) 166, 820
PpuMI RGGWCCY 1 cut(s) 585
PscI ACATGT 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 340
Psp5II RGGWCCY 1 cut(s) 585
PspN4I GGNNCC 3 cut(s) 330, 587, 681
PspPI GGNCC 2 cut(s) 329, 585
PspPPI RGGWCCY 1 cut(s) 585
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 480
SacI GAGCTC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 806
SatI GCNGC 2 cut(s) 624, 870
Sau3AI GATC 2 cut(s) 511, 993
Sau96I GGNCC 2 cut(s) 329, 585
SchI GAGTC 2 cut(s) 167, 821
SduI GDGCHC 2 cut(s) 340, 715
SfaNI GCATC 5 cut(s) 171, 447, 662, 715, 926
SfcI CTRYAG 1 cut(s) 978
Sfr274I CTCGAG 1 cut(s) 155
SinI GGWCC 2 cut(s) 329, 585
SlaI CTCGAG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 5 cut(s) 196, 462, 567, 641, 774
SspI AATATT 1 cut(s) 596
SspMI CTAG 3 cut(s) 701, 843, 941
SstI GAGCTC 1 cut(s) 340
StyI CCWWGG 2 cut(s) 495, 581
TaaI ACNGT 2 cut(s) 392, 403
TaqI TCGA 3 cut(s) 156, 352, 810
TasI AATT 5 cut(s) 196, 462, 567, 641, 774
TatI WGTACW 1 cut(s) 472
Tru1I TTAA 1 cut(s) 806
Tru9I TTAA 1 cut(s) 806
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 623, 869
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 654, 915
VpaK11BI GGWCC 2 cut(s) 329, 585
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 2 cut(s) 196, 641
XceI RCATGY 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 155
XspI CTAG 3 cut(s) 701, 843, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.