RchiOBHm_Chr5g0050911

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007924
Physical Location & Seq
Forward (+)
51628488 .. 51635752
7265 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32843

Sequence Viewer

Length: 1038 bp
ATGGCGTCGGCGTCGGCGTCGTCGTCGTCTTCAGCTCTCTCAAGACTGTCCAGTATCAGCAGACACTTCAATCCCTCCCACCCAAACAAGGACGGTGTGGCCTTAATCAAGATTTCTCCAGAGGTTTCCGAAGCTTTGTCAATTGGGCGTGCCGTCGTTGCTCTTGAATCCACCATTATTTCCCATGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCAATTGTCAGGGAAAATGGGGCTGTTCCTGCCACCATTGCTATCTTGGATGGCACACCTTGCATAGGTCTAACTATAGAAGAACTGGAGAGGCTTGCCAGATTGGGTCCAAGAGCTCAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGGCAACCAGAGGGAACGGTGCAACTACTGTTTCTGCAACCATGATTTTTGCTCATATGGTTGGCATCCCATTGTTTGTAACGGGAGGAATTGGAGGAGTACATCAACATGGCGAGCATACAATGGACATATCTTCTGATCTTACTGAGCTTGGAAGGACACCTGTAGCTGTCATCTCTGCTGGTGTAAAATCAATTTTGGATATCCCAAGGACCCTCGAATATTTGGAAACACAGGGAGTTTGTGTAGCTGCTTACAAGACCAATGAATTTCCAGCATTTTTCACAGAAGCAAGTGGCTGCAAGGTGCCTTGCCGTGTAGATACCCCAGATGATTGCGCTCGGCTTATAGATGCAAACCTTAAACTTGAGCTTGGAACTGGAATGTTGATTGCTGTTCCAATTCCTAAAGAACACTCGGCTTCTGGAAATTTAATTGAGTCTGCAATACAGACAGCTCTTAGAGAAGCTAGGGATAAGAACATAACAGGCAATGCTGCTACTCCATTCTTGCTTGCAAGAGTAAATGAACTAACTGGAGGAGCATCTTTGGCTTCGAACATTGCCCTGGTGAAGAATAATGCACTTGTTGGTTCTAAAATTGCTGTAGCACTTTCCCAGATCAGAGAATGTTGTAGAAAAGGTGGTTTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

345

Amino Acids

36.13

Weight (kDa)

6.76

Isoelectric Point (pI)

26.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 41 - 331 2.5e-131 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 688
AcsI RAATTY 3 cut(s) 205, 650, 811
AcuI CTGAAG 1 cut(s) 15
AcyI GRCGYC 3 cut(s) 5, 11, 17
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 2 cut(s) 88, 296
AflIII ACRYGT 1 cut(s) 376
AgsI TTSAA 2 cut(s) 70, 167
AjnI CCWGG 1 cut(s) 948
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 2 cut(s) 206, 360
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 3 cut(s) 632, 681, 878
ApoI RAATTY 3 cut(s) 205, 650, 811
AspLEI GCGC 1 cut(s) 722
AspS9I GGNCC 2 cut(s) 338, 594
AsuHPI GGTGA 1 cut(s) 964
AsuII TTCGAA 1 cut(s) 938
AvaII GGWCC 2 cut(s) 338, 594
BanI GGYRCC 1 cut(s) 688
BanII GRGCYC 1 cut(s) 349
BbsI GAAGAC 2 cut(s) 21, 359
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 3 cut(s) 619, 668, 865
BccI CCATC 1 cut(s) 275
BceAI ACGGC 3 cut(s) 137, 178, 681
BciT130I CCWGG 1 cut(s) 950
BciVI GTATCC 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 206, 360
BfaI CTAG 1 cut(s) 852
BfmI CTRYAG 3 cut(s) 306, 546, 987
BfuI GTATCC 1 cut(s) 207
BisI GCNGC 3 cut(s) 633, 682, 879
BlsI GCNGC 3 cut(s) 634, 683, 880
Bme1390I CCNGG 1 cut(s) 950
Bme18I GGWCC 2 cut(s) 338, 594
BmgT120I GGNCC 2 cut(s) 338, 594
BmiI GGNNCC 3 cut(s) 339, 596, 690
BmrFI CCNGG 1 cut(s) 950
BmsI GCATC 4 cut(s) 180, 456, 724, 935
BpiI GAAGAC 2 cut(s) 21, 359
BpmI CTGGAG 3 cut(s) 102, 338, 939
Bpu14I TTCGAA 1 cut(s) 938
BpuEI CTTGAG 2 cut(s) 25, 770
BsaHI GRCGYC 3 cut(s) 5, 11, 17
BsaJI CCNNGG 3 cut(s) 184, 590, 948
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 296
Bse1I ACTGG 4 cut(s) 51, 321, 766, 922
Bse3DI GCAATG 4 cut(s) 267, 369, 880, 942
BseBI CCWGG 1 cut(s) 950
BseDI CCNNGG 3 cut(s) 184, 590, 948
BseGI GGATG 3 cut(s) 195, 286, 447
BseLI CCNNNNNNNGG 2 cut(s) 88, 296
BseMI GCAATG 4 cut(s) 267, 369, 880, 942
BseMII CTCAG 2 cut(s) 362, 519
BseNI ACTGG 4 cut(s) 51, 321, 766, 922
BseRI GAGGAG 2 cut(s) 492, 936
BseXI GCAGC 3 cut(s) 619, 668, 865
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 688
BsiHKAI GWGCWC 1 cut(s) 349
BslI CCNNNNNNNGG 2 cut(s) 88, 296
BsmAI GTCTC 2 cut(s) 206, 360
BsnI GGCC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 938
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 2 cut(s) 520, 1002
Bsp19I CCATGG 1 cut(s) 184
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 361, 520
BspLI GGNNCC 3 cut(s) 339, 596, 690
BspT104I TTCGAA 1 cut(s) 938
BspT107I GGYRCC 1 cut(s) 688
BsrDI GCAATG 4 cut(s) 267, 369, 880, 942
BsrI ACTGG 4 cut(s) 51, 321, 766, 922
BssECI CCNNGG 3 cut(s) 184, 590, 948
BssMI GATC 2 cut(s) 520, 1002
BssNI GRCGYC 3 cut(s) 5, 11, 17
BssT1I CCWWGG 2 cut(s) 184, 590
Bst2UI CCWGG 1 cut(s) 950
Bst4CI ACNGT 4 cut(s) 48, 95, 401, 412
Bst6I CTCTTC 1 cut(s) 358
BstACI GRCGYC 3 cut(s) 5, 11, 17
BstAPI GCANNNNNTGC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 938
BstC8I GCNNGC 4 cut(s) 150, 327, 497, 897
BstDEI CTNAG 3 cut(s) 348, 528, 842
BstDSI CCRYGG 1 cut(s) 184
BstENI CCTNNNNNAGG 1 cut(s) 294
BstF5I GGATG 3 cut(s) 195, 286, 447
BstHHI GCGC 1 cut(s) 722
BstKTI GATC 2 cut(s) 523, 1005
BstMAI GTCTC 2 cut(s) 206, 360
BstMBI GATC 2 cut(s) 520, 1002
BstMWI GCNNNNNNNGC 5 cut(s) 158, 260, 269, 291, 932
BstNI CCWGG 1 cut(s) 950
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 948
BstSFI CTRYAG 3 cut(s) 306, 546, 987
BstV1I GCAGC 3 cut(s) 619, 668, 865
BstV2I GAAGAC 2 cut(s) 21, 359
BstXI CCANNNNNNTGG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 207
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 3 cut(s) 195, 286, 447
Cac8I GCNNGC 4 cut(s) 150, 327, 497, 897
CfoI GCGC 1 cut(s) 722
Cfr13I GGNCC 2 cut(s) 338, 594
CseI GACGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 482
CviAII CATG 4 cut(s) 185, 377, 424, 491
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 3 cut(s) 348, 528, 842
DpnI GATC 2 cut(s) 522, 1004
DpnII GATC 2 cut(s) 520, 1002
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 184, 590
Eco24I GRGCYC 1 cut(s) 349
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 338, 594
Eco53kI GAGCTC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 15
EcoICRI GAGCTC 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 594
EcoRII CCWGG 1 cut(s) 948
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 2 cut(s) 184, 590
EcoT38I GRGCYC 1 cut(s) 349
ErhI CCWWGG 2 cut(s) 184, 590
FaeI CATG 4 cut(s) 188, 380, 427, 494
FatI CATG 4 cut(s) 184, 376, 423, 490
FauNDI CATATG 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 633, 682, 879
FokI GGATG 3 cut(s) 202, 293, 434
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 3 cut(s) 633, 682, 879
FspBI CTAG 1 cut(s) 852
GlaI GCGC 1 cut(s) 721
GluI GCNGC 3 cut(s) 633, 682, 879
GsuI CTGGAG 3 cut(s) 102, 338, 939
HaeIII GGCC 1 cut(s) 101
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 722
Hin1I GRCGYC 3 cut(s) 5, 11, 17
Hin1II CATG 4 cut(s) 188, 380, 427, 494
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 2 cut(s) 167, 821
HphI GGTGA 1 cut(s) 964
Hpy188I TCNGA 4 cut(s) 130, 351, 520, 1007
Hpy188III TCNNGA 5 cut(s) 42, 109, 119, 164, 807
Hpy99I CGWCG 6 cut(s) 10, 16, 22, 25, 28, 158
HpyAV CCTTC 1 cut(s) 531
HpyCH4III ACNGT 4 cut(s) 48, 95, 401, 412
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 260, 269, 291, 932
HpyF3I CTNAG 3 cut(s) 348, 528, 842
Hsp92I GRCGYC 3 cut(s) 5, 11, 17
Hsp92II CATG 4 cut(s) 188, 380, 427, 494
HspAI GCGC 1 cut(s) 720
Kzo9I GATC 2 cut(s) 520, 1002
LmnI GCTCC 1 cut(s) 923
Lsp1109I GCAGC 3 cut(s) 619, 668, 865
LweI GCATC 4 cut(s) 180, 456, 724, 935
MaeI CTAG 1 cut(s) 852
MaeIII GTNAC 1 cut(s) 460
MalI GATC 2 cut(s) 522, 1004
MboI GATC 2 cut(s) 520, 1002
MboII GAAGA 6 cut(s) 21, 323, 364, 375, 507, 967
MfeI CAATTG 2 cut(s) 141, 234
MhlI GDGCHC 1 cut(s) 349
MlyI GAGTC 1 cut(s) 830
MseI TTAA 3 cut(s) 104, 744, 815
MslI CAYNNNNRTG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 950
MunI CAATTG 2 cut(s) 141, 234
MvaI CCWGG 1 cut(s) 950
MwoI GCNNNNNNNGC 5 cut(s) 158, 260, 269, 291, 932
NcoI CCATGG 1 cut(s) 184
NdeI CATATG 1 cut(s) 438
NdeII GATC 2 cut(s) 520, 1002
NlaIII CATG 4 cut(s) 188, 380, 427, 494
NlaIV GGNNCC 3 cut(s) 339, 596, 690
NmeAIII GCCGAG 2 cut(s) 703, 779
NspI RCATGY 1 cut(s) 380
NspV TTCGAA 1 cut(s) 938
PciI ACATGT 1 cut(s) 376
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 20
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 3 cut(s) 634, 683, 880
PleI GAGTC 1 cut(s) 829
PpsI GAGTC 1 cut(s) 829
PpuMI RGGWCCY 1 cut(s) 594
PscI ACATGT 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 948
PspGI CCWGG 1 cut(s) 948
PspN4I GGNNCC 3 cut(s) 339, 596, 690
PspPI GGNCC 2 cut(s) 338, 594
PspPPI RGGWCCY 1 cut(s) 594
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 489
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 3 cut(s) 104, 744, 815
SatI GCNGC 3 cut(s) 633, 682, 879
Sau3AI GATC 2 cut(s) 520, 1002
Sau96I GGNCC 2 cut(s) 338, 594
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 1 cut(s) 950
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 4 cut(s) 180, 456, 724, 935
SfcI CTRYAG 3 cut(s) 306, 546, 987
SfuI TTCGAA 1 cut(s) 938
SinI GGWCC 2 cut(s) 338, 594
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 40, 749
SmoI CTYRAG 2 cut(s) 40, 749
SspI AATATT 1 cut(s) 605
SspMI CTAG 1 cut(s) 852
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 948
StyI CCWWGG 2 cut(s) 184, 590
TaaI ACNGT 4 cut(s) 48, 95, 401, 412
TaqI TCGA 3 cut(s) 361, 600, 938
TatI WGTACW 1 cut(s) 481
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 3 cut(s) 104, 744, 815
Tru9I TTAA 3 cut(s) 104, 744, 815
TseI GCWGC 3 cut(s) 632, 681, 878
TspDTI ATGAA 2 cut(s) 663, 924
VpaK11BI GGWCC 2 cut(s) 338, 594
XagI CCTNNNNNAGG 1 cut(s) 294
XapI RAATTY 3 cut(s) 205, 650, 811
XceI RCATGY 1 cut(s) 380
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XspI CTAG 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.