Rw5G031760

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007924
Physical Location & Seq
Forward (+)
52705561 .. 52720669
15109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031760.1

Sequence Viewer

Length: 870 bp
ATGGCGTCGTCATCGTCGTCTTCAGCTCTCTCAAGACTGTCCAGTATCAGCAGACACTTCAATCCCTCCCACGCAAACAAGGACGGTGTGGCCTTAATCAAGATTTCTCCAGAGGTTTCCGAAGCTTTGTCAATTGGGCGTGCCGTCGTTGCTCTTGAATCCACCATTATTTCCCATGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCAATTGTCAGGGAAAATGGGGCTGTTCCTGCCACCATTGCTATCTTGGATGGCACACCTTGCATAGGTCTAACTATAGAAGAGCTGGAGAGGCTTGCCAGATTGGGTCCAAGAGCTCAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGGCAACCAGAGGGAACGGTGCAACTACTGTTTCTGCAACCATGATTTTTGCTCATATGGTTGGCATCCCATTGTTTGTAACGGGAGGAATTGGAGGAGTACATCAACATGGCGAGCATACAATGGACATATCTTCTGATCTTACTGAGCTTGGAAGGACACCTGTAGCTGTCATCTCTGCTGGTGTAAAATCAATTTTGGATATCCCAAGGACCCTCGAATATTTGGAAACACAGGGAGTTTGTGTAGCTGCTTACAAGACCAATGAATTTCCAGCATTTTTCACAGAAGCAAGTGGCTGCAAGGTGCCTTGCCGTGTAGATACCCCAGATGATTGCGCTCGGCTTATAGATGCAAACCTTAAACTTGAGCTTGGAACTGGAATGTTGATTGCTGTTCCAATTCCTAAAGAACACTCGGCTTCTGGAAATTTAATTGAGTCTGCAATACAGACTGCTCTCAGAGAAGCTAGGTGGACTTGTTCTGCCCGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

289

Amino Acids

30.51

Weight (kDa)

6.31

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 38 - 282 2.6e-111 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 679
AcsI RAATTY 3 cut(s) 196, 641, 802
AcuI CTGAAG 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 2 cut(s) 61, 158
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 2 cut(s) 197, 351
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 2 cut(s) 623, 672
ApoI RAATTY 3 cut(s) 196, 641, 802
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 2 cut(s) 329, 585
AvaII GGWCC 2 cut(s) 329, 585
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 12, 350
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 2 cut(s) 610, 659
BccI CCATC 1 cut(s) 266
BceAI ACGGC 3 cut(s) 128, 169, 672
BciVI GTATCC 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 197, 351
BfaI CTAG 1 cut(s) 843
BfmI CTRYAG 2 cut(s) 297, 537
BfuI GTATCC 1 cut(s) 198
BisI GCNGC 2 cut(s) 624, 673
BlsI GCNGC 2 cut(s) 625, 674
Bme18I GGWCC 2 cut(s) 329, 585
BmgT120I GGNCC 2 cut(s) 329, 585
BmiI GGNNCC 3 cut(s) 330, 587, 681
BmsI GCATC 3 cut(s) 171, 447, 715
BpiI GAAGAC 2 cut(s) 12, 350
BpmI CTGGAG 2 cut(s) 93, 329
BpuEI CTTGAG 2 cut(s) 16, 761
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 175, 581
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 42, 757
Bse3DI GCAATG 2 cut(s) 258, 360
BseDI CCNNGG 2 cut(s) 175, 581
BseGI GGATG 3 cut(s) 186, 277, 438
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 2 cut(s) 258, 360
BseMII CTCAG 3 cut(s) 353, 510, 847
BseNI ACTGG 2 cut(s) 42, 757
BseRI GAGGAG 1 cut(s) 483
BseXI GCAGC 2 cut(s) 610, 659
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 679
BsiHKAI GWGCWC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 2 cut(s) 197, 351
BsnI GGCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 1 cut(s) 511
Bsp19I CCATGG 1 cut(s) 175
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 3 cut(s) 352, 511, 846
BspLI GGNNCC 3 cut(s) 330, 587, 681
BspQI GCTCTTC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 679
BsrDI GCAATG 2 cut(s) 258, 360
BsrI ACTGG 2 cut(s) 42, 757
BssECI CCNNGG 2 cut(s) 175, 581
BssMI GATC 1 cut(s) 511
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 175, 581
Bst4CI ACNGT 4 cut(s) 39, 86, 392, 403
Bst6I CTCTTC 2 cut(s) 297, 349
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 141, 318, 488
BstDEI CTNAG 3 cut(s) 339, 519, 833
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 3 cut(s) 186, 277, 438
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 1 cut(s) 514
BstMAI GTCTC 2 cut(s) 197, 351
BstMBI GATC 1 cut(s) 511
BstMWI GCNNNNNNNGC 5 cut(s) 149, 251, 260, 282, 313
BstNSI RCATGY 1 cut(s) 371
BstSFI CTRYAG 2 cut(s) 297, 537
BstV1I GCAGC 2 cut(s) 610, 659
BstV2I GAAGAC 2 cut(s) 12, 350
BstXI CCANNNNNNTGG 1 cut(s) 199
BsuI GTATCC 1 cut(s) 198
BsuRI GGCC 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 3 cut(s) 186, 277, 438
Cac8I GCNNGC 3 cut(s) 141, 318, 488
CfoI GCGC 1 cut(s) 713
Cfr13I GGNCC 2 cut(s) 329, 585
Csp6I GTAC 1 cut(s) 473
CviAII CATG 4 cut(s) 176, 368, 415, 482
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 3 cut(s) 339, 519, 833
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
Eam1104I CTCTTC 2 cut(s) 297, 349
EarI CTCTTC 2 cut(s) 297, 349
Ecl136II GAGCTC 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 175, 581
Eco24I GRGCYC 1 cut(s) 340
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 329, 585
Eco53kI GAGCTC 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 338
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 585
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 2 cut(s) 175, 581
EcoT38I GRGCYC 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 175, 581
FaeI CATG 4 cut(s) 179, 371, 418, 485
FatI CATG 4 cut(s) 175, 367, 414, 481
FauNDI CATATG 1 cut(s) 429
Fnu4HI GCNGC 2 cut(s) 624, 673
FokI GGATG 3 cut(s) 193, 284, 425
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 2 cut(s) 624, 673
FspBI CTAG 1 cut(s) 843
GlaI GCGC 1 cut(s) 712
GluI GCNGC 2 cut(s) 624, 673
GsuI CTGGAG 2 cut(s) 93, 329
HaeIII GGCC 1 cut(s) 92
HhaI GCGC 1 cut(s) 713
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 179, 371, 418, 485
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 2 cut(s) 158, 812
Hpy166II GTNNAC 1 cut(s) 849
Hpy188I TCNGA 4 cut(s) 121, 342, 511, 836
Hpy188III TCNNGA 5 cut(s) 33, 100, 110, 155, 798
Hpy8I GTNNAC 1 cut(s) 849
Hpy99I CGWCG 3 cut(s) 10, 19, 149
HpyAV CCTTC 1 cut(s) 522
HpyCH4III ACNGT 4 cut(s) 39, 86, 392, 403
HpyCH4V TGCA 9 cut(s) 209, 285, 365, 395, 410, 675, 728, 818, 866
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 251, 260, 282, 313
HpyF3I CTNAG 3 cut(s) 339, 519, 833
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 179, 371, 418, 485
HspAI GCGC 1 cut(s) 711
Kzo9I GATC 1 cut(s) 511
LguI GCTCTTC 1 cut(s) 297
Lsp1109I GCAGC 2 cut(s) 610, 659
LweI GCATC 3 cut(s) 171, 447, 715
MaeI CTAG 1 cut(s) 843
MaeIII GTNAC 1 cut(s) 451
MalI GATC 1 cut(s) 513
MboI GATC 1 cut(s) 511
MboII GAAGA 5 cut(s) 12, 314, 355, 366, 498
MfeI CAATTG 2 cut(s) 132, 225
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 9 cut(s) 132, 196, 225, 462, 567, 641, 774, 802, 807
MlyI GAGTC 1 cut(s) 821
MnlI CCTC 9 cut(s) 76, 106, 208, 214, 306, 377, 452, 461, 599
MseI TTAA 3 cut(s) 95, 735, 806
MslI CAYNNNNRTG 1 cut(s) 480
MunI CAATTG 2 cut(s) 132, 225
MwoI GCNNNNNNNGC 5 cut(s) 149, 251, 260, 282, 313
NcoI CCATGG 1 cut(s) 175
NdeI CATATG 1 cut(s) 429
NdeII GATC 1 cut(s) 511
NlaIII CATG 4 cut(s) 179, 371, 418, 485
NlaIV GGNNCC 3 cut(s) 330, 587, 681
NmeAIII GCCGAG 2 cut(s) 694, 770
NspI RCATGY 1 cut(s) 371
PciI ACATGT 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 2 cut(s) 625, 674
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
PpuMI RGGWCCY 1 cut(s) 585
PscI ACATGT 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 340
Psp5II RGGWCCY 1 cut(s) 585
PspN4I GGNNCC 3 cut(s) 330, 587, 681
PspPI GGNCC 2 cut(s) 329, 585
PspPPI RGGWCCY 1 cut(s) 585
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 480
SacI GAGCTC 1 cut(s) 340
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 95, 735, 806
SatI GCNGC 2 cut(s) 624, 673
Sau3AI GATC 1 cut(s) 511
Sau96I GGNCC 2 cut(s) 329, 585
SchI GAGTC 1 cut(s) 821
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 3 cut(s) 171, 447, 715
SfcI CTRYAG 2 cut(s) 297, 537
SinI GGWCC 2 cut(s) 329, 585
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 2 cut(s) 31, 740
SmoI CTYRAG 2 cut(s) 31, 740
Sse9I AATT 9 cut(s) 132, 196, 225, 462, 567, 641, 774, 802, 807
SspI AATATT 1 cut(s) 596
SspMI CTAG 1 cut(s) 843
SstI GAGCTC 1 cut(s) 340
StyI CCWWGG 2 cut(s) 175, 581
TaaI ACNGT 4 cut(s) 39, 86, 392, 403
TaqI TCGA 2 cut(s) 352, 591
TasI AATT 9 cut(s) 132, 196, 225, 462, 567, 641, 774, 802, 807
TatI WGTACW 1 cut(s) 472
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 3 cut(s) 95, 735, 806
Tru9I TTAA 3 cut(s) 95, 735, 806
TseI GCWGC 2 cut(s) 623, 672
TspDTI ATGAA 1 cut(s) 654
VpaK11BI GGWCC 2 cut(s) 329, 585
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 3 cut(s) 196, 641, 802
XceI RCATGY 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XspI CTAG 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.