Rh5DG359400

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007924
Physical Location & Seq
Forward (+)
53582287 .. 53589477
7191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG359400.1

Sequence Viewer

Length: 972 bp
ATGGCGTCGGCGTCGGCGTCGTCGTCGTCTTCAGCTCTCTCAAGACTGTCCAGTATCAGCAGACACTTCAATCCCTCCCACCCAAACAAGGTGACCTCCCAATTCACTTTATCTCTCTCTCTCTCTCTCTCTCTTGCTAACCTGTTTTCCAAATTTCACACTGGGAGCTATCTTGTTTTCAAGGACGGTGTGGCCTTAATCAAGATTTCTCCAGAGGTTTCCGAAGCTTTGTCAATTGGGCGTGCCGTCGTTGCTCTTGAATCCACCATTATTTCCCATGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCAATTGTCAGGGAAAATGGGGCTGTTCCTGCCACCATTGCTATCTTGGATGGCACACCTTGCATAGGTCTAACTATAGAAGAACTGGAGAGGCTTGCCAGATTGGGTCCAAGAGCTCAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGGCAACCAGAGGGAACGGTGCAACTACTGTTTCTGCAACCATGATTTTTGCTCATATGGTTGGCATCCCATTGTTTGTAACGGGAGGAATTGGAGGAGTACATCAACATGGCGAGCATACAATGGACATATCTTCTGATCTTACTGAGCTTGGAAGGACACCTGTAGCTGTCATCTCTGCTGGTGTAAAATCAATTTTGGATATCCCAAGGACCCTCGAATATTTGGAAACACAGGGAGTTTGTGTAGCTGCTTACAAGACCAATGAATTTCCAGCATTTTTCACAGAAGCAAGTGGCTGCAAGGTGCCTTGCCGTGTAGATACCCCAGATGATTGCGCTCGGCTTATAGATGCAAACCTTAAACTTGAGCTTGGAACTGGAATGTTGATTGCTGTTCCAATTCCTAAAGAACACTCGGCTTCTGGAAATTTAATTGAGTCTGCAATACAGACAGCTCTTAGAGAAGCTAGGTGGACTTGTTCTGCCCGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

323

Amino Acids

34.19

Weight (kDa)

6.8

Isoelectric Point (pI)

24.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 72 - 316 3.8e-111 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 781
AcsI RAATTY 4 cut(s) 152, 298, 743, 904
AcuI CTGAAG 1 cut(s) 15
AcyI GRCGYC 3 cut(s) 5, 11, 17
AfaI GTAC 1 cut(s) 576
AfiI CCNNNNNNNGG 2 cut(s) 88, 389
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 70, 181, 260
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 2 cut(s) 299, 453
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 2 cut(s) 725, 774
ApoI RAATTY 4 cut(s) 152, 298, 743, 904
AspLEI GCGC 1 cut(s) 815
AspS9I GGNCC 2 cut(s) 431, 687
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 2 cut(s) 431, 687
BanI GGYRCC 1 cut(s) 781
BanII GRGCYC 1 cut(s) 442
BbsI GAAGAC 2 cut(s) 21, 452
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 2 cut(s) 712, 761
BccI CCATC 1 cut(s) 368
BceAI ACGGC 3 cut(s) 230, 271, 774
BciVI GTATCC 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 299, 453
BfaI CTAG 1 cut(s) 945
BfmI CTRYAG 2 cut(s) 399, 639
BfuI GTATCC 1 cut(s) 300
BisI GCNGC 2 cut(s) 726, 775
BlsI GCNGC 2 cut(s) 727, 776
Bme18I GGWCC 2 cut(s) 431, 687
BmgT120I GGNCC 2 cut(s) 431, 687
BmiI GGNNCC 3 cut(s) 432, 689, 783
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 3 cut(s) 273, 549, 817
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 2 cut(s) 21, 452
BpmI CTGGAG 2 cut(s) 195, 431
BpuEI CTTGAG 2 cut(s) 25, 863
BsaHI GRCGYC 3 cut(s) 5, 11, 17
BsaJI CCNNGG 2 cut(s) 277, 683
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 389
Bse1I ACTGG 4 cut(s) 51, 166, 414, 859
Bse3DI GCAATG 2 cut(s) 360, 462
BseDI CCNNGG 2 cut(s) 277, 683
BseGI GGATG 3 cut(s) 288, 379, 540
BseLI CCNNNNNNNGG 2 cut(s) 88, 389
BseMI GCAATG 2 cut(s) 360, 462
BseMII CTCAG 2 cut(s) 455, 612
BseNI ACTGG 4 cut(s) 51, 166, 414, 859
BseRI GAGGAG 1 cut(s) 585
BseXI GCAGC 2 cut(s) 712, 761
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 781
BsiHKAI GWGCWC 1 cut(s) 442
BslI CCNNNNNNNGG 2 cut(s) 88, 389
BsmAI GTCTC 2 cut(s) 299, 453
BsnI GGCC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 1 cut(s) 613
Bsp19I CCATGG 1 cut(s) 277
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 454, 613
BspLI GGNNCC 3 cut(s) 432, 689, 783
BspT107I GGYRCC 1 cut(s) 781
BsrDI GCAATG 2 cut(s) 360, 462
BsrI ACTGG 4 cut(s) 51, 166, 414, 859
BssECI CCNNGG 2 cut(s) 277, 683
BssMI GATC 1 cut(s) 613
BssNI GRCGYC 3 cut(s) 5, 11, 17
BssT1I CCWWGG 2 cut(s) 277, 683
Bst4CI ACNGT 4 cut(s) 48, 188, 494, 505
Bst6I CTCTTC 1 cut(s) 451
BstACI GRCGYC 3 cut(s) 5, 11, 17
BstAPI GCANNNNNTGC 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 243, 420, 590
BstDEI CTNAG 3 cut(s) 441, 621, 935
BstDSI CCRYGG 1 cut(s) 277
BstEII GGTNACC 1 cut(s) 91
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 288, 379, 540
BstHHI GCGC 1 cut(s) 815
BstKTI GATC 1 cut(s) 616
BstMAI GTCTC 2 cut(s) 299, 453
BstMBI GATC 1 cut(s) 613
BstMWI GCNNNNNNNGC 4 cut(s) 251, 353, 362, 384
BstNSI RCATGY 1 cut(s) 473
BstPI GGTNACC 1 cut(s) 91
BstSFI CTRYAG 2 cut(s) 399, 639
BstV1I GCAGC 2 cut(s) 712, 761
BstV2I GAAGAC 2 cut(s) 21, 452
BstXI CCANNNNNNTGG 1 cut(s) 301
BsuI GTATCC 1 cut(s) 300
BsuRI GGCC 1 cut(s) 194
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 3 cut(s) 288, 379, 540
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 3 cut(s) 243, 420, 590
CfoI GCGC 1 cut(s) 815
Cfr13I GGNCC 2 cut(s) 431, 687
CseI GACGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 575
CviAII CATG 4 cut(s) 278, 470, 517, 584
CviQI GTAC 1 cut(s) 575
DdeI CTNAG 3 cut(s) 441, 621, 935
DpnI GATC 1 cut(s) 615
DpnII GATC 1 cut(s) 613
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
Ecl136II GAGCTC 1 cut(s) 440
Eco130I CCWWGG 2 cut(s) 277, 683
Eco24I GRGCYC 1 cut(s) 442
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 2 cut(s) 431, 687
Eco53kI GAGCTC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 15
Eco91I GGTNACC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 440
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 687
EcoO65I GGTNACC 1 cut(s) 91
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 2 cut(s) 277, 683
EcoT38I GRGCYC 1 cut(s) 442
ErhI CCWWGG 2 cut(s) 277, 683
FaeI CATG 4 cut(s) 281, 473, 520, 587
FatI CATG 4 cut(s) 277, 469, 516, 583
FauNDI CATATG 1 cut(s) 531
Fnu4HI GCNGC 2 cut(s) 726, 775
FokI GGATG 3 cut(s) 295, 386, 527
FriOI GRGCYC 1 cut(s) 442
Fsp4HI GCNGC 2 cut(s) 726, 775
FspBI CTAG 1 cut(s) 945
GlaI GCGC 1 cut(s) 814
GluI GCNGC 2 cut(s) 726, 775
GsuI CTGGAG 2 cut(s) 195, 431
HaeIII GGCC 1 cut(s) 194
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 815
Hin1I GRCGYC 3 cut(s) 5, 11, 17
Hin1II CATG 4 cut(s) 281, 473, 520, 587
Hin6I GCGC 1 cut(s) 813
HinP1I GCGC 1 cut(s) 813
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 2 cut(s) 260, 914
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 951
Hpy188I TCNGA 3 cut(s) 223, 444, 613
Hpy188III TCNNGA 5 cut(s) 42, 202, 212, 257, 900
Hpy8I GTNNAC 1 cut(s) 951
Hpy99I CGWCG 6 cut(s) 10, 16, 22, 25, 28, 251
HpyAV CCTTC 1 cut(s) 624
HpyCH4III ACNGT 4 cut(s) 48, 188, 494, 505
HpyCH4V TGCA 9 cut(s) 311, 387, 467, 497, 512, 777, 830, 920, 968
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 353, 362, 384
HpyF3I CTNAG 3 cut(s) 441, 621, 935
Hsp92I GRCGYC 3 cut(s) 5, 11, 17
Hsp92II CATG 4 cut(s) 281, 473, 520, 587
HspAI GCGC 1 cut(s) 813
Kzo9I GATC 1 cut(s) 613
LmnI GCTCC 1 cut(s) 165
Lsp1109I GCAGC 2 cut(s) 712, 761
LweI GCATC 3 cut(s) 273, 549, 817
MaeI CTAG 1 cut(s) 945
MaeIII GTNAC 2 cut(s) 91, 553
MalI GATC 1 cut(s) 615
MboI GATC 1 cut(s) 613
MboII GAAGA 5 cut(s) 21, 416, 457, 468, 600
MfeI CAATTG 2 cut(s) 234, 327
MhlI GDGCHC 1 cut(s) 442
MlyI GAGTC 1 cut(s) 923
MseI TTAA 3 cut(s) 197, 837, 908
MslI CAYNNNNRTG 1 cut(s) 582
MunI CAATTG 2 cut(s) 234, 327
MwoI GCNNNNNNNGC 4 cut(s) 251, 353, 362, 384
NcoI CCATGG 1 cut(s) 277
NdeI CATATG 1 cut(s) 531
NdeII GATC 1 cut(s) 613
NlaIII CATG 4 cut(s) 281, 473, 520, 587
NlaIV GGNNCC 3 cut(s) 432, 689, 783
NmeAIII GCCGAG 2 cut(s) 796, 872
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 1 cut(s) 473
PciI ACATGT 1 cut(s) 469
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 20
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 2 cut(s) 727, 776
PleI GAGTC 1 cut(s) 922
PpsI GAGTC 1 cut(s) 922
PpuMI RGGWCCY 1 cut(s) 687
PscI ACATGT 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 687
PspEI GGTNACC 1 cut(s) 91
PspN4I GGNNCC 3 cut(s) 432, 689, 783
PspPI GGNCC 2 cut(s) 431, 687
PspPPI RGGWCCY 1 cut(s) 687
RsaI GTAC 1 cut(s) 576
RsaNI GTAC 1 cut(s) 575
RseI CAYNNNNRTG 1 cut(s) 582
SacI GAGCTC 1 cut(s) 442
SaqAI TTAA 3 cut(s) 197, 837, 908
SatI GCNGC 2 cut(s) 726, 775
Sau3AI GATC 1 cut(s) 613
Sau96I GGNCC 2 cut(s) 431, 687
SchI GAGTC 1 cut(s) 923
SduI GDGCHC 1 cut(s) 442
SfaNI GCATC 3 cut(s) 273, 549, 817
SfcI CTRYAG 2 cut(s) 399, 639
SinI GGWCC 2 cut(s) 431, 687
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 40, 842
SmoI CTYRAG 2 cut(s) 40, 842
SspI AATATT 1 cut(s) 698
SspMI CTAG 1 cut(s) 945
SstI GAGCTC 1 cut(s) 442
StyI CCWWGG 2 cut(s) 277, 683
TaaI ACNGT 4 cut(s) 48, 188, 494, 505
TaqI TCGA 2 cut(s) 454, 693
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 3 cut(s) 197, 837, 908
Tru9I TTAA 3 cut(s) 197, 837, 908
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 2 cut(s) 725, 774
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 756
TspRI CASTG 1 cut(s) 166
VpaK11BI GGWCC 2 cut(s) 431, 687
XagI CCTNNNNNAGG 1 cut(s) 387
XapI RAATTY 4 cut(s) 152, 298, 743, 904
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XspI CTAG 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.