MD11G1196200.v1.1

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007924
Physical Location & Seq
Forward (+)
28103917 .. 28117554
13638 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1196200.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGCGTCGTCCTCTTCTTCTTCATCAGCTGCTATCTCAAGACTCTCTAATCTCCGCAAACACCTCCATCCCTCGCATTTGGACAAGGGTGGCATCGGATCAATCAAGATTTCTCCAGAGGTTTCTGACGCTTTGTCCAATGGCCGTGCTGTTGTCGCTCTCGAATCCACCATTATTTCCCATGGGATGCCGTATCCTAAGAACTTGGAAACAGCAAAGGAGGTTGAGGCAGTTGTGAGGGAAAATGGAGCTGTTCCTGCCACTGTTGCTATTTTGGATGGCATACCTTGCATAGGTCTGAGTATGGAAGAATTGGAGAAGCTTGCCAATCTGGGTCATAAAGCTCAGAAGACAGCTCGAAGGGATATTGCACATGTTGTGGCAACCAGAGGGACTGGAGCAACTACTGTTTCTGCAACCATGTTTTTTGCTTCCATGGTTGGCATCCCATTGTTTGTTACTGGAGGAATTGGAGGAGTACATAGACATGGCGAGCATACAATGGACATATCTTCTGATCTTACTGAGCTTGGAAGGACACCAGTAGCTGTCATCTCTGCTGGTGTAAAATCGATATTGGATATCCCAAGGACCCTTGAATATTTGGAGACTCAGGGAGTTTGCGTTGCTGCTTATAGGACCAACGAATTTCCAGCATTTTTTACAGAAACGAGTGGTTGTAAGGTGCCTTGCCGTTTAGATACCCCAGAAGACTGTGCTCAGCTTATAGATGCAAATCTGAAACTTGAGCTTGGAGGTGGGATTTTGATTGCTGTTCCAATTCCTAAAGAGCACTCAGCTTCTGGAAGTTTAATTGAGTCTGCAATACAGAGAGCCCTTGACGAAGCTAGGGATAAGAACATAATAGGAAATGATGCAACTCCATTCTTGCTCAAGAGAGTGAATGAGCTAACGGGAGGAGCTTCATTGGCTTCAAACATTGCACTGGTGAAGAACAATGCACTTGTTGGAGCTAAAGTTGCTGTAGCACTTGCGAAGACTAGAGAACGCAAAAATAAAGGTGGTGCAGAGTCGGCGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

348

Amino Acids

36.27

Weight (kDa)

7.15

Isoelectric Point (pI)

27.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 40 - 330 3.3e-131 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 685
AciI CCGC 1 cut(s) 55
AclWI GGATC 1 cut(s) 106
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 2 cut(s) 599, 936
AhdI GACNNNNNGTC 1 cut(s) 133
Alw21I GWGCWC 2 cut(s) 721, 795
Alw26I GTCTC 1 cut(s) 602
AlwI GGATC 1 cut(s) 106
AlwNI CAGNNNCTG 2 cut(s) 548, 803
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 2 cut(s) 29, 629
ApoI RAATTY 1 cut(s) 647
AspS9I GGNCC 2 cut(s) 591, 639
AsuHPI GGTGA 1 cut(s) 961
AvaII GGWCC 2 cut(s) 591, 639
BanI GGYRCC 1 cut(s) 685
BanII GRGCYC 1 cut(s) 838
BbsI GAAGAC 3 cut(s) 356, 717, 1004
Bbv12I GWGCWC 2 cut(s) 721, 795
BbvI GCAGC 2 cut(s) 16, 616
BccI CCATC 2 cut(s) 75, 272
BceAI ACGGC 3 cut(s) 129, 175, 678
BciVI GTATCC 1 cut(s) 204
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 2 cut(s) 849, 1002
BfmI CTRYAG 1 cut(s) 984
BfuI GTATCC 1 cut(s) 204
BisI GCNGC 2 cut(s) 30, 630
BlpI GCTNAGC 1 cut(s) 720
BlsI GCNGC 2 cut(s) 31, 631
Bme18I GGWCC 2 cut(s) 591, 639
BmeRI GACNNNNNGTC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 591, 639
BmiI GGNNCC 2 cut(s) 593, 687
BmsI GCATC 5 cut(s) 102, 177, 453, 721, 865
BpiI GAAGAC 3 cut(s) 356, 717, 1004
BpmI CTGGAG 3 cut(s) 99, 417, 483
Bpu1102I GCTNAGC 1 cut(s) 720
BpuEI CTTGAG 3 cut(s) 22, 767, 878
Bsa29I ATCGAT 1 cut(s) 572
BsaBI GATNNNNATC 1 cut(s) 735
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 181, 435, 587
BsaXI ACNNNNNCTCC 4 cut(s) 609, 639, 963, 993
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 4 cut(s) 400, 466, 542, 951
Bse3DI GCAATG 1 cut(s) 939
Bse8I GATNNNNATC 1 cut(s) 735
BseCI ATCGAT 1 cut(s) 572
BseDI CCNNGG 3 cut(s) 181, 435, 587
BseGI GGATG 4 cut(s) 67, 192, 283, 444
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 6 cut(s) 290, 359, 516, 626, 734, 810
BseNI ACTGG 4 cut(s) 400, 466, 542, 951
BseRI GAGGAG 2 cut(s) 489, 933
BseXI GCAGC 2 cut(s) 16, 616
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 1 cut(s) 685
BshVI ATCGAT 1 cut(s) 572
BsiHKAI GWGCWC 2 cut(s) 721, 795
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 602
BsmFI GGGAC 1 cut(s) 406
BsnI GGCC 1 cut(s) 144
Bsp1286I GDGCHC 3 cut(s) 721, 795, 838
Bsp143I GATC 2 cut(s) 98, 517
Bsp1720I GCTNAGC 1 cut(s) 720
Bsp19I CCATGG 2 cut(s) 181, 435
BspACI CCGC 1 cut(s) 55
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 6 cut(s) 291, 358, 517, 625, 733, 809
BspDI ATCGAT 1 cut(s) 572
BspLI GGNNCC 2 cut(s) 593, 687
BspPI GGATC 1 cut(s) 106
BspT107I GGYRCC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 939
BsrI ACTGG 4 cut(s) 400, 466, 542, 951
BssECI CCNNGG 3 cut(s) 181, 435, 587
BssMI GATC 2 cut(s) 98, 517
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 181, 435, 587
Bst4CI ACNGT 3 cut(s) 265, 409, 716
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 324, 494
BstDEI CTNAG 7 cut(s) 198, 299, 345, 525, 612, 720, 796
BstDSI CCRYGG 2 cut(s) 181, 435
BstENI CCTNNNNNAGG 1 cut(s) 291
BstF5I GGATG 4 cut(s) 67, 192, 283, 444
BstKTI GATC 2 cut(s) 101, 520
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 2 cut(s) 98, 517
BstMWI GCNNNNNNNGC 7 cut(s) 155, 257, 266, 288, 929, 980, 1034
BstNSI RCATGY 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 984
BstV1I GCAGC 2 cut(s) 16, 616
BstV2I GAAGAC 3 cut(s) 356, 717, 1004
Bsu15I ATCGAT 1 cut(s) 572
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 1 cut(s) 144
BsuTUI ATCGAT 1 cut(s) 572
BtgI CCRYGG 2 cut(s) 181, 435
BtsCI GGATG 4 cut(s) 67, 192, 283, 444
BtsIMutI CAGTG 2 cut(s) 261, 944
Cac8I GCNNGC 2 cut(s) 324, 494
CaiI CAGNNNCTG 2 cut(s) 548, 803
Cfr13I GGNCC 2 cut(s) 591, 639
ClaI ATCGAT 1 cut(s) 572
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 479
CviAII CATG 5 cut(s) 182, 374, 421, 436, 488
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 7 cut(s) 198, 299, 345, 525, 612, 720, 796
DpnI GATC 2 cut(s) 100, 519
DpnII GATC 2 cut(s) 98, 517
DriI GACNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 181, 435, 587
Eco24I GRGCYC 1 cut(s) 838
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 2 cut(s) 591, 639
EcoNI CCTNNNNNAGG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 591
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 3 cut(s) 181, 435, 587
EcoT38I GRGCYC 1 cut(s) 838
ErhI CCWWGG 3 cut(s) 181, 435, 587
FaeI CATG 5 cut(s) 185, 377, 424, 439, 491
FaqI GGGAC 1 cut(s) 406
FatI CATG 5 cut(s) 181, 373, 420, 435, 487
Fnu4HI GCNGC 2 cut(s) 30, 630
FokI GGATG 4 cut(s) 54, 199, 290, 431
FriOI GRGCYC 1 cut(s) 838
Fsp4HI GCNGC 2 cut(s) 30, 630
FspBI CTAG 2 cut(s) 849, 1002
GluI GCNGC 2 cut(s) 30, 630
GsuI CTGGAG 3 cut(s) 99, 417, 483
HaeIII GGCC 1 cut(s) 144
HgaI GACGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 185, 377, 424, 439, 491
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 5 cut(s) 42, 164, 610, 818, 1031
HphI GGTGA 1 cut(s) 961
Hpy188I TCNGA 6 cut(s) 98, 127, 300, 348, 517, 741
Hpy188III TCNNGA 6 cut(s) 39, 106, 116, 161, 804, 895
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 354, 528
HpyCH4III ACNGT 3 cut(s) 265, 409, 716
HpyCH4V TGCA 9 cut(s) 291, 371, 416, 734, 824, 878, 944, 962, 1028
HpyF10VI GCNNNNNNNGC 7 cut(s) 155, 257, 266, 288, 929, 980, 1034
HpyF3I CTNAG 7 cut(s) 198, 299, 345, 525, 612, 720, 796
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 185, 377, 424, 439, 491
Kzo9I GATC 2 cut(s) 98, 517
LmnI GCTCC 4 cut(s) 248, 398, 920, 971
Lsp1109I GCAGC 2 cut(s) 16, 616
LweI GCATC 5 cut(s) 102, 177, 453, 721, 865
MaeI CTAG 2 cut(s) 849, 1002
MaeIII GTNAC 1 cut(s) 457
MalI GATC 2 cut(s) 100, 519
MboI GATC 2 cut(s) 98, 517
MboII GAAGA 9 cut(s) 6, 9, 12, 320, 361, 504, 722, 964, 1009
MhlI GDGCHC 3 cut(s) 721, 795, 838
MluCI AATT 5 cut(s) 311, 468, 647, 780, 813
MlyI GAGTC 4 cut(s) 36, 604, 827, 1040
MmeI TCCRAC 1 cut(s) 949
MseI TTAA 1 cut(s) 812
MslI CAYNNNNRTG 1 cut(s) 486
MspA1I CMGCKG 1 cut(s) 29
MwoI GCNNNNNNNGC 7 cut(s) 155, 257, 266, 288, 929, 980, 1034
NcoI CCATGG 2 cut(s) 181, 435
NdeII GATC 2 cut(s) 98, 517
NlaIII CATG 5 cut(s) 185, 377, 424, 439, 491
NlaIV GGNNCC 2 cut(s) 593, 687
NspI RCATGY 1 cut(s) 377
PciI ACATGT 1 cut(s) 373
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 2 cut(s) 31, 631
PleI GAGTC 4 cut(s) 36, 604, 826, 1039
PpsI GAGTC 4 cut(s) 36, 604, 826, 1039
PpuMI RGGWCCY 1 cut(s) 591
PscI ACATGT 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 591
PspN4I GGNNCC 2 cut(s) 593, 687
PspPI GGNCC 2 cut(s) 591, 639
PspPPI RGGWCCY 1 cut(s) 591
PstNI CAGNNNCTG 2 cut(s) 548, 803
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 1 cut(s) 812
SatI GCNGC 2 cut(s) 30, 630
Sau3AI GATC 2 cut(s) 98, 517
Sau96I GGNCC 2 cut(s) 591, 639
SchI GAGTC 4 cut(s) 36, 604, 827, 1040
SduI GDGCHC 3 cut(s) 721, 795, 838
SfaNI GCATC 5 cut(s) 102, 177, 453, 721, 865
SfcI CTRYAG 1 cut(s) 984
SinI GGWCC 2 cut(s) 591, 639
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 3 cut(s) 37, 746, 893
SmoI CTYRAG 3 cut(s) 37, 746, 893
Sse9I AATT 5 cut(s) 311, 468, 647, 780, 813
SsiI CCGC 1 cut(s) 55
SspI AATATT 1 cut(s) 602
SspMI CTAG 2 cut(s) 849, 1002
StyI CCWWGG 3 cut(s) 181, 435, 587
TaaI ACNGT 3 cut(s) 265, 409, 716
TaqI TCGA 3 cut(s) 162, 358, 572
TasI AATT 5 cut(s) 311, 468, 647, 780, 813
TatI WGTACW 1 cut(s) 478
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 812
Tru9I TTAA 1 cut(s) 812
TscAI CASTG 2 cut(s) 268, 951
TseI GCWGC 2 cut(s) 29, 629
TspDTI ATGAA 2 cut(s) 12, 915
TspRI CASTG 2 cut(s) 268, 951
VpaK11BI GGWCC 2 cut(s) 591, 639
XagI CCTNNNNNAGG 1 cut(s) 291
XapI RAATTY 1 cut(s) 647
XceI RCATGY 1 cut(s) 377
XspI CTAG 2 cut(s) 849, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.