Rroxscaffold_3G00229240

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007924
Physical Location & Seq
Reverse (-)
13706366 .. 13709848
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229240.1

Sequence Viewer

Length: 531 bp
ATGGTTTCCTTGCTAACAGTCCGTGTTTTGCGAGGAACTATTTCCTCGTCAAAAGAAGCATTTAGCGAGGGAAATACACTTTCCTCGCTATTTCATTTGACGATCGAGTGGTATCAATCGAAGCATGGCATCGGCGTCGGCGTCGTCTTCGACTCTCTCAAGACTGTCCGATGTCAGCGGACACTTCAATCCCTCCCACGCAAACAAGGACGGTGTGGCCTTAATCAAGATTTCTCCGAGGTTTCCGAAGCTTTGTCAAATGGGCGTCTTTGTCGTTGCTCTCGAATCCACCATTATTCCCACGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCAATTGTCAAGGAAAATGGGGCTGTTCGTGCCACCATTGCTATCTTGGATGGCACACCTTGCATAGAAGAACTGGAGAGGCTTGCCGATTGGGTCCAAGAACTCGAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGACAACCGGAGGGAACGGTGCAACTACGTTTCTGCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

176

Amino Acids

19.47

Weight (kDa)

8.31

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 96 - 172 2e-21 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 178
AcsI RAATTY 1 cut(s) 322
AcyI GRCGYC 3 cut(s) 135, 141, 265
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 1 cut(s) 188
AluBI AGCT 2 cut(s) 251, 464
AluI AGCT 2 cut(s) 251, 464
Alw26I GTCTC 2 cut(s) 323, 465
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 322
Asp700I GAANNNNTTC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 442
BbsI GAAGAC 2 cut(s) 139, 464
BccI CCATC 1 cut(s) 392
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciVI GTATCC 1 cut(s) 324
BcoDI GTCTC 2 cut(s) 323, 465
BfuI GTATCC 1 cut(s) 324
Bme18I GGWCC 1 cut(s) 442
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 1 cut(s) 443
BmsI GCATC 2 cut(s) 138, 297
BpiI GAAGAC 2 cut(s) 139, 464
BpmI CTGGAG 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 143
BsaHI GRCGYC 3 cut(s) 135, 141, 265
BsaJI CCNNGG 2 cut(s) 237, 301
BsaWI WCCGGW 1 cut(s) 494
Bse1I ACTGG 1 cut(s) 426
Bse3DI GCAATG 2 cut(s) 384, 474
BseDI CCNNGG 2 cut(s) 237, 301
BseGI GGATG 2 cut(s) 312, 403
BseMI GCAATG 2 cut(s) 384, 474
BseNI ACTGG 1 cut(s) 426
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 1 cut(s) 495
BsmAI GTCTC 2 cut(s) 323, 465
BsnI GGCC 1 cut(s) 219
BsoBI CYCGRG 1 cut(s) 452
Bsp143I GATC 1 cut(s) 102
BspACI CCGC 1 cut(s) 178
BspANI GGCC 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 443
BsrDI GCAATG 2 cut(s) 384, 474
BsrI ACTGG 1 cut(s) 426
BssECI CCNNGG 2 cut(s) 237, 301
BssMI GATC 1 cut(s) 102
BssNI GRCGYC 3 cut(s) 135, 141, 265
Bst4CI ACNGT 4 cut(s) 19, 166, 213, 506
Bst6I CTCTTC 1 cut(s) 463
BstACI GRCGYC 3 cut(s) 135, 141, 265
BstAPI GCANNNNNTGC 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 432
BstDSI CCRYGG 1 cut(s) 301
BstF5I GGATG 2 cut(s) 312, 403
BstKTI GATC 1 cut(s) 105
BstMAI GTCTC 2 cut(s) 323, 465
BstMBI GATC 1 cut(s) 102
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 377, 386, 408
BstNSI RCATGY 1 cut(s) 485
BstV2I GAAGAC 2 cut(s) 139, 464
BstXI CCANNNNNNTGG 1 cut(s) 325
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 301
BtsCI GGATG 2 cut(s) 312, 403
Cac8I GCNNGC 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 442
CseI GACGC 3 cut(s) 124, 130, 254
CviAII CATG 3 cut(s) 125, 482, 528
CviJI RGCY 5 cut(s) 219, 251, 371, 430, 464
CviKI_1 RGCY 5 cut(s) 219, 251, 371, 430, 464
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 442
Eco88I CYCGRG 1 cut(s) 452
FaeI CATG 3 cut(s) 128, 485, 531
FaiI YATR 4 cut(s) 126, 413, 483, 529
FatI CATG 3 cut(s) 124, 481, 527
FokI GGATG 2 cut(s) 319, 410
GsuI CTGGAG 1 cut(s) 443
HaeIII GGCC 1 cut(s) 219
HapII CCGG 1 cut(s) 495
HgaI GACGC 3 cut(s) 124, 130, 254
Hin1I GRCGYC 3 cut(s) 135, 141, 265
Hin1II CATG 3 cut(s) 128, 485, 531
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 2 cut(s) 152, 285
HpaII CCGG 1 cut(s) 495
Hpy188I TCNGA 3 cut(s) 170, 238, 247
Hpy188III TCNNGA 4 cut(s) 160, 227, 282, 454
Hpy99I CGWCG 2 cut(s) 140, 146
HpyCH4III ACNGT 4 cut(s) 19, 166, 213, 506
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 5 cut(s) 335, 411, 479, 509, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 386, 408
HpySE526I ACGT 1 cut(s) 515
Hsp92I GRCGYC 3 cut(s) 135, 141, 265
Hsp92II CATG 3 cut(s) 128, 485, 531
Kzo9I GATC 1 cut(s) 102
LpnPI CCDG 2 cut(s) 407, 508
LweI GCATC 2 cut(s) 138, 297
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 1 cut(s) 487
MalI GATC 1 cut(s) 104
MboI GATC 1 cut(s) 102
MboII GAAGA 4 cut(s) 139, 428, 469, 480
MfeI CAATTG 1 cut(s) 351
MluCI AATT 2 cut(s) 322, 351
MlyI GAGTC 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 40
MseI TTAA 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 1 cut(s) 495
MunI CAATTG 1 cut(s) 351
MwoI GCNNNNNNNGC 3 cut(s) 377, 386, 408
NdeII GATC 1 cut(s) 102
NlaIII CATG 3 cut(s) 128, 485, 531
NlaIV GGNNCC 1 cut(s) 443
NmuCI GTSAC 1 cut(s) 487
NspI RCATGY 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 452
PciI ACATGT 1 cut(s) 481
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 280
PdmI GAANNNNTTC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 285
Ple19I CGATCG 1 cut(s) 105
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PscI ACATGT 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 1 cut(s) 442
PvuI CGATCG 1 cut(s) 105
SaqAI TTAA 1 cut(s) 222
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 1 cut(s) 146
SetI ASST 6 cut(s) 243, 253, 345, 409, 466, 518
SfaNI GCATC 2 cut(s) 138, 297
Sfr274I CTCGAG 1 cut(s) 452
SinI GGWCC 1 cut(s) 442
SlaI CTCGAG 1 cut(s) 452
SmlI CTYRAG 2 cut(s) 158, 452
SmoI CTYRAG 2 cut(s) 158, 452
Sse9I AATT 2 cut(s) 322, 351
SsiI CCGC 1 cut(s) 178
TaaI ACNGT 4 cut(s) 19, 166, 213, 506
TaiI ACGT 1 cut(s) 518
TaqI TCGA 6 cut(s) 105, 119, 150, 283, 453, 466
TasI AATT 2 cut(s) 322, 351
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TseFI GTSAC 1 cut(s) 487
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 1 cut(s) 11
VpaK11BI GGWCC 1 cut(s) 442
XapI RAATTY 1 cut(s) 322
XceI RCATGY 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XhoI CTCGAG 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.