Rh5CG374100

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007924
Physical Location & Seq
Forward (+)
48172609 .. 48180688
8080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG374100.1

Sequence Viewer

Length: 942 bp
ATGGCGTCGTCTTCAGCTCTCTCAAGACTGTACAGTATCAGCAGACACTTCAATCCCTCCCACGCAAACAAGGACGGTGTGGCCTTAATCAAGATTTCTCCAGAGGTTTCCGAAGCTTTGTCAAATGGGCGTGCCGTCGTTGCTCTTGAATCCACCATTATTTCCCATGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCAATTGTCAGGGAAAATGGGGCTGTTCCTGCCACCATTGCTATCTTGGATGGCACACCTTGCATAGGTCTAACTATAGAAGAACTGGAGAGGCTTGCCAGATTGGGTCCAAGAGCTCAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGGCAACCAGAGGGAACGGTGCAACTACTGTTTCTGCAACCATGATTTTTGCTCATATGGTTGGCATCCCATTGTTTGTAACAGGAGGAATTGGAGGAGTACATCAACATGGCGAGCATACAATGGACATATCTTCTGATCTTACTGAGCTTGGAAGGACACCTGTAGCTGTCATCTCTGCTGGTGTAAAATCAATTTTGGATATCCCAAGGACCCTCGAATATTTGGAAACACAGGGAGTTTGTGTAGCTGCTTACAAGACCAATGAATTTCCAGCATTTTTCACAGAAGCAAGTGGCTGCAAGGTGCCTTGCCGTGTAGATACCCCAGATGATTGCGCTTGGCTTATAGGTAATTCGTTATTTTACCTAGAAGAAATTGTATCGAAGTTTAGAGTAATACCTTCTCTTCTCAAATATTTTCATTTTACAGATGCAAACCTTAAACTTGAGCTTGGAACTGGAATGTTGATTGCTGTTCCAATTCCTAAAGAACACTCGGCTTCTGGAAATTTAATTGAGTCTGCAATACAGACAGCTCTTAGAGAAGCTAGGTGGACTTGTTCTGCCCGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

313

Amino Acids

33.59

Weight (kDa)

6.37

Isoelectric Point (pI)

26.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 35 - 238 2.6e-98 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 670
AcsI RAATTY 3 cut(s) 187, 632, 874
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 32, 465
AfiI CCNNNNNNNGG 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 2 cut(s) 52, 149
Alw21I GWGCWC 1 cut(s) 331
Alw26I GTCTC 2 cut(s) 188, 342
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 614, 663
ApoI RAATTY 3 cut(s) 187, 632, 874
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 2 cut(s) 320, 576
AvaII GGWCC 2 cut(s) 320, 576
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 3, 341
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 2 cut(s) 601, 650
BccI CCATC 1 cut(s) 257
BceAI ACGGC 3 cut(s) 119, 160, 663
BciVI GTATCC 1 cut(s) 189
BcoDI GTCTC 2 cut(s) 188, 342
BfaI CTAG 2 cut(s) 734, 915
BfmI CTRYAG 2 cut(s) 288, 528
BfuI GTATCC 1 cut(s) 189
BisI GCNGC 2 cut(s) 615, 664
BlsI GCNGC 2 cut(s) 616, 665
Bme18I GGWCC 2 cut(s) 320, 576
BmgT120I GGNCC 2 cut(s) 320, 576
BmiI GGNNCC 3 cut(s) 321, 578, 672
BmsI GCATC 3 cut(s) 162, 438, 787
BpiI GAAGAC 2 cut(s) 3, 341
BpmI CTGGAG 2 cut(s) 84, 320
BpuEI CTTGAG 2 cut(s) 7, 833
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 166, 572
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 303, 829
Bse3DI GCAATG 2 cut(s) 249, 351
BseDI CCNNGG 2 cut(s) 166, 572
BseGI GGATG 3 cut(s) 177, 268, 429
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMI GCAATG 2 cut(s) 249, 351
BseMII CTCAG 2 cut(s) 344, 501
BseNI ACTGG 2 cut(s) 303, 829
BseRI GAGGAG 1 cut(s) 474
BseXI GCAGC 2 cut(s) 601, 650
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 670
BsiHKAI GWGCWC 1 cut(s) 331
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 188, 342
BsnI GGCC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 1 cut(s) 502
Bsp19I CCATGG 1 cut(s) 166
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 2 cut(s) 343, 502
BspLI GGNNCC 3 cut(s) 321, 578, 672
BspT107I GGYRCC 1 cut(s) 670
BsrDI GCAATG 2 cut(s) 249, 351
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 2 cut(s) 303, 829
BssECI CCNNGG 2 cut(s) 166, 572
BssMI GATC 1 cut(s) 502
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 166, 572
Bst4CI ACNGT 5 cut(s) 30, 35, 77, 383, 394
Bst6I CTCTTC 2 cut(s) 340, 777
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 30
BstC8I GCNNGC 3 cut(s) 132, 309, 479
BstDEI CTNAG 3 cut(s) 330, 510, 905
BstDSI CCRYGG 1 cut(s) 166
BstENI CCTNNNNNAGG 1 cut(s) 276
BstF5I GGATG 3 cut(s) 177, 268, 429
BstHHI GCGC 1 cut(s) 704
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 2 cut(s) 188, 342
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 4 cut(s) 140, 242, 251, 273
BstNSI RCATGY 1 cut(s) 362
BstSFI CTRYAG 2 cut(s) 288, 528
BstV1I GCAGC 2 cut(s) 601, 650
BstV2I GAAGAC 2 cut(s) 3, 341
BstXI CCANNNNNNTGG 1 cut(s) 190
BsuI GTATCC 1 cut(s) 189
BsuRI GGCC 1 cut(s) 83
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 3 cut(s) 177, 268, 429
Cac8I GCNNGC 3 cut(s) 132, 309, 479
CfoI GCGC 1 cut(s) 704
Cfr13I GGNCC 2 cut(s) 320, 576
Csp6I GTAC 2 cut(s) 31, 464
CviAII CATG 4 cut(s) 167, 359, 406, 473
CviQI GTAC 2 cut(s) 31, 464
DdeI CTNAG 3 cut(s) 330, 510, 905
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
Eam1104I CTCTTC 2 cut(s) 340, 777
EarI CTCTTC 2 cut(s) 340, 777
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 166, 572
Eco24I GRGCYC 1 cut(s) 331
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 2 cut(s) 320, 576
Eco53kI GAGCTC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 576
EcoRV GATATC 1 cut(s) 568
EcoT14I CCWWGG 2 cut(s) 166, 572
EcoT38I GRGCYC 1 cut(s) 331
ErhI CCWWGG 2 cut(s) 166, 572
FaeI CATG 4 cut(s) 170, 362, 409, 476
FatI CATG 4 cut(s) 166, 358, 405, 472
FauNDI CATATG 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 615, 664
FokI GGATG 3 cut(s) 184, 275, 416
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 2 cut(s) 615, 664
FspBI CTAG 2 cut(s) 734, 915
GlaI GCGC 1 cut(s) 703
GluI GCNGC 2 cut(s) 615, 664
GsuI CTGGAG 2 cut(s) 84, 320
HaeIII GGCC 1 cut(s) 83
HhaI GCGC 1 cut(s) 704
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 170, 362, 409, 476
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HindIII AAGCTT 1 cut(s) 114
HinfI GANTC 2 cut(s) 149, 884
Hpy166II GTNNAC 1 cut(s) 921
Hpy188I TCNGA 3 cut(s) 112, 333, 502
Hpy188III TCNNGA 5 cut(s) 24, 91, 101, 146, 870
Hpy8I GTNNAC 1 cut(s) 921
Hpy99I CGWCG 2 cut(s) 10, 140
HpyAV CCTTC 2 cut(s) 513, 777
HpyCH4III ACNGT 5 cut(s) 30, 35, 77, 383, 394
HpyCH4V TGCA 9 cut(s) 200, 276, 356, 386, 401, 666, 800, 890, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 242, 251, 273
HpyF3I CTNAG 3 cut(s) 330, 510, 905
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 170, 362, 409, 476
HspAI GCGC 1 cut(s) 702
Kzo9I GATC 1 cut(s) 502
Lsp1109I GCAGC 2 cut(s) 601, 650
LweI GCATC 3 cut(s) 162, 438, 787
MaeI CTAG 2 cut(s) 734, 915
MaeIII GTNAC 1 cut(s) 442
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 7 cut(s) 3, 305, 346, 357, 489, 749, 764
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 331
MlyI GAGTC 1 cut(s) 893
MnlI CCTC 9 cut(s) 67, 97, 199, 205, 297, 368, 443, 452, 590
MseI TTAA 3 cut(s) 86, 807, 878
MslI CAYNNNNRTG 1 cut(s) 471
MunI CAATTG 1 cut(s) 216
MwoI GCNNNNNNNGC 4 cut(s) 140, 242, 251, 273
NcoI CCATGG 1 cut(s) 166
NdeI CATATG 1 cut(s) 420
NdeII GATC 1 cut(s) 502
NlaIII CATG 4 cut(s) 170, 362, 409, 476
NlaIV GGNNCC 3 cut(s) 321, 578, 672
NmeAIII GCCGAG 1 cut(s) 842
NspI RCATGY 1 cut(s) 362
PciI ACATGT 1 cut(s) 358
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 2 cut(s) 616, 665
PleI GAGTC 1 cut(s) 892
PpsI GAGTC 1 cut(s) 892
PpuMI RGGWCCY 1 cut(s) 576
PscI ACATGT 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 576
PspN4I GGNNCC 3 cut(s) 321, 578, 672
PspPI GGNCC 2 cut(s) 320, 576
PspPPI RGGWCCY 1 cut(s) 576
RsaI GTAC 2 cut(s) 32, 465
RsaNI GTAC 2 cut(s) 31, 464
RseI CAYNNNNRTG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 86, 807, 878
SatI GCNGC 2 cut(s) 615, 664
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 2 cut(s) 320, 576
SchI GAGTC 1 cut(s) 893
SduI GDGCHC 1 cut(s) 331
SfaNI GCATC 3 cut(s) 162, 438, 787
SfcI CTRYAG 2 cut(s) 288, 528
SinI GGWCC 2 cut(s) 320, 576
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 2 cut(s) 22, 812
SmoI CTYRAG 2 cut(s) 22, 812
SspI AATATT 2 cut(s) 587, 782
SspMI CTAG 2 cut(s) 734, 915
SstI GAGCTC 1 cut(s) 331
StyI CCWWGG 2 cut(s) 166, 572
TaaI ACNGT 5 cut(s) 30, 35, 77, 383, 394
TaqI TCGA 3 cut(s) 343, 582, 749
TatI WGTACW 2 cut(s) 30, 463
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 3 cut(s) 86, 807, 878
Tru9I TTAA 3 cut(s) 86, 807, 878
TseI GCWGC 2 cut(s) 614, 663
TspDTI ATGAA 2 cut(s) 645, 776
VpaK11BI GGWCC 2 cut(s) 320, 576
XagI CCTNNNNNAGG 1 cut(s) 276
XapI RAATTY 3 cut(s) 187, 632, 874
XceI RCATGY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XspI CTAG 2 cut(s) 734, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.