Rroxscaffold_1G00029820

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007924
Physical Location & Seq
Reverse (-)
38790092 .. 38796470
6379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029820.1

Sequence Viewer

Length: 1101 bp
ATGGCGTCGGCGTCGGCGTCAGCTCTCTCAAGACTGTCCGGTATCAGCAGACACTTCAATCCCTCCCACGCAAACAAGGGCGGTGTGGCCTTAATCAAGATTTCTCCAGAGGTTTCCGAAGCTTTGTCAAATGGGCGTGCCGTCGTTGCTCTTGAATCCACCATTATTTCCCATGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCAATTGTCAGGGAAAATGGGGCTGTTCCTGCCACCATTGCTATCTTGGATGGCACACCTTGCATAGGTCTAACTATAGAAGAACTGGAGAGGCTTGCCAGATTGGGTCCAAGAACTCAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGGCAACCAGAGGGAACGGTGCAACTACTGTTTCTGCAACCATGATTTTTGCTCATATGGTTGGCATCCCATTGTTTGTAACGGGAGGAATTGGAGGAGTACATCAACATGGCGAGCATACAATGGACATATCTTCTGATCTTACTGAGCTTGGAAGGACACCTGTAGCTGTCATCTCTGCTGGTGTAAAATCAATTTTGGATATCCCAAGGACCCTCGAATATTTGGAAACACAGGGAGTTTGTGTAGCTGCTTACAAGACCAATGAATTTCCAGCATTTTTCACAGAAGCAAGTGGCTGCAAGGTGCCTTGCCGTGTTGATACCCCAGATGATTGCGCTCGGCTTATAGATGCAAACCTTAAACTTGAGCTTGGAACTGGAATGCTGATTGCTGTTCCAATTCCAAAAGAACACTCGGCTTCTGGAAATTTAATTGAGTCTGCAATACAGACAGCTCTTAGAGAAGCTAGGGATAAGAACATAACAGGCAATGCTGCTACTCCATTCTTGCTTGCAAGAGTAAATGAACTAACTGGAGGAGCATCTTTGGCTTCGAACATTGCCCTGGTGAAGAATAATGCACTTGTTGGTTCTAAAATTGCTGTAGCACTTTCCCAGATCAGAGAATGTTGTAGAAAAGACTGGTCATGCGATATGGTAGTCAGAAAGAGACAAAACAAAGCTGCACTAAGTGGCTTGAGGTTGTTGAAAGGGCAGCCAATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

366

Amino Acids

38.62

Weight (kDa)

8.76

Isoelectric Point (pI)

26.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 37 - 327 5.9e-132 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 676
AciI CCGC 1 cut(s) 81
AcsI RAATTY 3 cut(s) 193, 638, 799
AcyI GRCGYC 3 cut(s) 5, 11, 17
AdeI CACNNNGTG 1 cut(s) 1064
AfaI GTAC 1 cut(s) 471
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 3 cut(s) 58, 155, 1081
AjnI CCWGG 1 cut(s) 936
Alw26I GTCTC 3 cut(s) 194, 348, 1036
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 5 cut(s) 620, 669, 866, 1055, 1087
ApoI RAATTY 3 cut(s) 193, 638, 799
AspLEI GCGC 1 cut(s) 710
AspS9I GGNCC 2 cut(s) 326, 582
AsuHPI GGTGA 1 cut(s) 952
AsuII TTCGAA 1 cut(s) 926
AvaII GGWCC 2 cut(s) 326, 582
BanI GGYRCC 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 347
BbvI GCAGC 4 cut(s) 607, 656, 853, 1042
BccI CCATC 1 cut(s) 263
BceAI ACGGC 3 cut(s) 125, 166, 669
BciT130I CCWGG 1 cut(s) 938
BciVI GTATCC 1 cut(s) 195
BcoDI GTCTC 3 cut(s) 194, 348, 1036
BfaI CTAG 1 cut(s) 840
BfmI CTRYAG 3 cut(s) 294, 534, 975
BfuI GTATCC 1 cut(s) 195
BisI GCNGC 5 cut(s) 621, 670, 867, 1056, 1088
BlsI GCNGC 5 cut(s) 622, 671, 868, 1057, 1089
Bme1390I CCNGG 1 cut(s) 938
Bme18I GGWCC 2 cut(s) 326, 582
BmgT120I GGNCC 2 cut(s) 326, 582
BmiI GGNNCC 3 cut(s) 327, 584, 678
BmrFI CCNGG 1 cut(s) 938
BmsI GCATC 4 cut(s) 168, 444, 712, 923
BpiI GAAGAC 1 cut(s) 347
BpmI CTGGAG 3 cut(s) 90, 326, 927
Bpu14I TTCGAA 1 cut(s) 926
BpuEI CTTGAG 3 cut(s) 13, 758, 1090
BsaHI GRCGYC 3 cut(s) 5, 11, 17
BsaJI CCNNGG 3 cut(s) 172, 578, 936
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 4 cut(s) 309, 754, 910, 1019
Bse3DI GCAATG 4 cut(s) 255, 357, 868, 930
BseBI CCWGG 1 cut(s) 938
BseDI CCNNGG 3 cut(s) 172, 578, 936
BseGI GGATG 3 cut(s) 183, 274, 435
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 4 cut(s) 255, 357, 868, 930
BseMII CTCAG 2 cut(s) 350, 507
BseNI ACTGG 4 cut(s) 309, 754, 910, 1019
BseRI GAGGAG 2 cut(s) 480, 924
BseXI GCAGC 4 cut(s) 607, 656, 853, 1042
BsgI GTGCAG 1 cut(s) 1041
BshFI GGCC 1 cut(s) 89
BshNI GGYRCC 1 cut(s) 676
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 3 cut(s) 194, 348, 1036
BsmI GAATGC 1 cut(s) 759
BsnI GGCC 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 926
Bsp143I GATC 2 cut(s) 508, 990
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 1 cut(s) 81
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 2 cut(s) 349, 508
BspLI GGNNCC 3 cut(s) 327, 584, 678
BspT104I TTCGAA 1 cut(s) 926
BspT107I GGYRCC 1 cut(s) 676
BsrDI GCAATG 4 cut(s) 255, 357, 868, 930
BsrI ACTGG 4 cut(s) 309, 754, 910, 1019
BssECI CCNNGG 3 cut(s) 172, 578, 936
BssMI GATC 2 cut(s) 508, 990
BssNI GRCGYC 3 cut(s) 5, 11, 17
BssT1I CCWWGG 2 cut(s) 172, 578
Bst2UI CCWGG 1 cut(s) 938
Bst4CI ACNGT 3 cut(s) 36, 389, 400
Bst6I CTCTTC 1 cut(s) 346
BstACI GRCGYC 3 cut(s) 5, 11, 17
BstAPI GCANNNNNTGC 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 926
BstC8I GCNNGC 4 cut(s) 138, 315, 485, 885
BstDEI CTNAG 4 cut(s) 336, 516, 830, 1061
BstDSI CCRYGG 1 cut(s) 172
BstENI CCTNNNNNAGG 1 cut(s) 282
BstF5I GGATG 3 cut(s) 183, 274, 435
BstHHI GCGC 1 cut(s) 710
BstKTI GATC 2 cut(s) 511, 993
BstMAI GTCTC 3 cut(s) 194, 348, 1036
BstMBI GATC 2 cut(s) 508, 990
BstMWI GCNNNNNNNGC 5 cut(s) 146, 248, 257, 279, 920
BstNI CCWGG 1 cut(s) 938
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 936
BstSFI CTRYAG 3 cut(s) 294, 534, 975
BstV1I GCAGC 4 cut(s) 607, 656, 853, 1042
BstV2I GAAGAC 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 196
BsuI GTATCC 1 cut(s) 195
BsuRI GGCC 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 3 cut(s) 183, 274, 435
Cac8I GCNNGC 4 cut(s) 138, 315, 485, 885
CfoI GCGC 1 cut(s) 710
Cfr13I GGNCC 2 cut(s) 326, 582
CseI GACGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 470
CviAII CATG 5 cut(s) 173, 365, 412, 479, 1020
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 4 cut(s) 336, 516, 830, 1061
DpnI GATC 2 cut(s) 510, 992
DpnII GATC 2 cut(s) 508, 990
DraIII CACNNNGTG 1 cut(s) 1064
Eam1104I CTCTTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 346
Eco130I CCWWGG 2 cut(s) 172, 578
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 2 cut(s) 326, 582
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 936
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 172, 578
ErhI CCWWGG 2 cut(s) 172, 578
FaeI CATG 5 cut(s) 176, 368, 415, 482, 1023
FatI CATG 5 cut(s) 172, 364, 411, 478, 1019
FauNDI CATATG 1 cut(s) 426
Fnu4HI GCNGC 5 cut(s) 621, 670, 867, 1056, 1088
FokI GGATG 3 cut(s) 190, 281, 422
Fsp4HI GCNGC 5 cut(s) 621, 670, 867, 1056, 1088
FspBI CTAG 1 cut(s) 840
GlaI GCGC 1 cut(s) 709
GluI GCNGC 5 cut(s) 621, 670, 867, 1056, 1088
GsuI CTGGAG 3 cut(s) 90, 326, 927
HaeIII GGCC 1 cut(s) 89
HapII CCGG 1 cut(s) 39
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 710
Hin1I GRCGYC 3 cut(s) 5, 11, 17
Hin1II CATG 5 cut(s) 176, 368, 415, 482, 1023
Hin6I GCGC 1 cut(s) 708
HinP1I GCGC 1 cut(s) 708
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 2 cut(s) 155, 809
HpaII CCGG 1 cut(s) 39
HphI GGTGA 1 cut(s) 952
Hpy188I TCNGA 5 cut(s) 118, 339, 508, 995, 1037
Hpy188III TCNNGA 5 cut(s) 30, 97, 107, 152, 795
Hpy99I CGWCG 3 cut(s) 10, 16, 146
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 3 cut(s) 36, 389, 400
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 248, 257, 279, 920
HpyF3I CTNAG 4 cut(s) 336, 516, 830, 1061
Hsp92I GRCGYC 3 cut(s) 5, 11, 17
Hsp92II CATG 5 cut(s) 176, 368, 415, 482, 1023
HspAI GCGC 1 cut(s) 708
Kzo9I GATC 2 cut(s) 508, 990
LmnI GCTCC 1 cut(s) 911
Lsp1109I GCAGC 4 cut(s) 607, 656, 853, 1042
LweI GCATC 4 cut(s) 168, 444, 712, 923
MaeI CTAG 1 cut(s) 840
MaeIII GTNAC 1 cut(s) 448
MalI GATC 2 cut(s) 510, 992
MboI GATC 2 cut(s) 508, 990
MboII GAAGA 5 cut(s) 311, 352, 363, 495, 955
MfeI CAATTG 1 cut(s) 222
MlyI GAGTC 1 cut(s) 818
MseI TTAA 4 cut(s) 92, 732, 803, 1099
MslI CAYNNNNRTG 1 cut(s) 477
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 1 cut(s) 938
MunI CAATTG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 759
MvaI CCWGG 1 cut(s) 938
MwoI GCNNNNNNNGC 5 cut(s) 146, 248, 257, 279, 920
NcoI CCATGG 1 cut(s) 172
NdeI CATATG 1 cut(s) 426
NdeII GATC 2 cut(s) 508, 990
NlaIII CATG 5 cut(s) 176, 368, 415, 482, 1023
NlaIV GGNNCC 3 cut(s) 327, 584, 678
NmeAIII GCCGAG 2 cut(s) 691, 767
NspI RCATGY 1 cut(s) 368
NspV TTCGAA 1 cut(s) 926
PciI ACATGT 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 759
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 5 cut(s) 622, 671, 868, 1057, 1089
PleI GAGTC 1 cut(s) 817
PpsI GAGTC 1 cut(s) 817
PpuMI RGGWCCY 1 cut(s) 582
PscI ACATGT 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 936
PspGI CCWGG 1 cut(s) 936
PspN4I GGNNCC 3 cut(s) 327, 584, 678
PspPI GGNCC 2 cut(s) 326, 582
PspPPI RGGWCCY 1 cut(s) 582
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 4 cut(s) 92, 732, 803, 1099
SatI GCNGC 5 cut(s) 621, 670, 867, 1056, 1088
Sau3AI GATC 2 cut(s) 508, 990
Sau96I GGNCC 2 cut(s) 326, 582
SchI GAGTC 1 cut(s) 818
ScrFI CCNGG 1 cut(s) 938
SfaNI GCATC 4 cut(s) 168, 444, 712, 923
SfcI CTRYAG 3 cut(s) 294, 534, 975
SfuI TTCGAA 1 cut(s) 926
SinI GGWCC 2 cut(s) 326, 582
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 3 cut(s) 28, 737, 1069
SmoI CTYRAG 3 cut(s) 28, 737, 1069
SsiI CCGC 1 cut(s) 81
SspI AATATT 1 cut(s) 593
SspMI CTAG 1 cut(s) 840
StyD4I CCNGG 1 cut(s) 936
StyI CCWWGG 2 cut(s) 172, 578
TaaI ACNGT 3 cut(s) 36, 389, 400
TaqI TCGA 3 cut(s) 349, 588, 926
TatI WGTACW 1 cut(s) 469
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 4 cut(s) 92, 732, 803, 1099
Tru9I TTAA 4 cut(s) 92, 732, 803, 1099
TseI GCWGC 5 cut(s) 620, 669, 866, 1055, 1087
TspDTI ATGAA 2 cut(s) 651, 912
VpaK11BI GGWCC 2 cut(s) 326, 582
XagI CCTNNNNNAGG 1 cut(s) 282
XapI RAATTY 3 cut(s) 193, 638, 799
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XspI CTAG 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.