FvH4_3g30540

Non-structural maintenance of chromosomes element 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003611
Physical Location & Seq
Reverse (-)
23817687 .. 23825441
7755 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30540.t4

Sequence Viewer

Length: 1080 bp
ATGGAGAATTGTACTGACCTGACTGACTTTCCAAATGCCCAGAGAAATCACGGCTCGCCGGAGACTTGGATTTTGGGTTTAGCCTGGGTCTGTTTTGCCCTTTTTTATATGAGATGTGAGGGTTTTGAAACAATGACGGAACTGAACGCCAAGCACCACACTCTGATTCAAGCCCTCCTCTCTCGCGGCCCACTCAAGGAGGCCCAATTCCACCGCATGTTCTCGGAGCTCACCGGAGAAAACCCAGGTGTTCGTCGGAAAGCGTTTGATGATTATCTTCTGAGGATAAACAAGGCCCTTTCGTATGTTCAGTTGGAGCTGCGAGGATGCAGGAATCAATATGATGGTCAGGTTTATTATGGAGTTGTCAATAATGTGTCTGATGAGGAATCCAAGCTGGGAACCACCTATTCGGTTGCTCAGATCGCTTTCTATAAGGCTATTATAGAAGCAATAGTGCAAGAAGCTGCTCAAGGAACCATATCTAGCAGCGATGCTCTTAATCTACGATTAGAGAATCAGGTTAACATTGGTACATCATCACAATCACAAGGTCAATTTCATATCCCTGCTGCATTGAAGAACTTTTCAATGTCCCAGAAGGAAAAAACTCTTGATGAATTTGTACGGGATAAATGGCTTTCCTTCACCGCTGAGGGTCATGTTGGACTTGGTGTCAGATCCTTCCTTGATCTGCGAAGTTGGTTTCGCAATAATGATGTTCCTTCATGTGAAGTGTGTAACGAAGCTGGGGTGAAGGCAGTGTTATGTCAAAAAGAGGATTGTAAGGTTCGAATTCATCAGTACTGCCTTGGAAAACTGTTTGCTAAGAAGAAGGGTGAAACAGTTTGTCCAAGTTGTGGTACTCAATGGCCATATACTAGACCAAAAGCAGAAGCTGTGGAAGATGATGAGCCAGATTGCGCTGCTCAGAGCCAACCACCTGTAGTACCCAGGAAAGAGAAGCTGAGAACAAGCCGAATTGGTGATGGAGATATAGTTGAATCTAGTTCCTCTCAGGCTTCCTTACCTGGTCGTAATCTAAGACGATCAACTCGAAATTCTATGCGCCCCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMC_Nse1 zf-RING-like

Protein Analysis

360

Amino Acids

40.36

Weight (kDa)

7.51

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_Nse1 PF07574 8 - 188 1.3e-47 Nse1 non-SMC component of SMC5-6 complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 860
AccII CGCG 1 cut(s) 186
AciI CCGC 3 cut(s) 186, 214, 651
AclWI GGATC 1 cut(s) 675
AcoI YGGCCR 1 cut(s) 872
AcsI RAATTY 3 cut(s) 620, 795, 1060
AfaI GTAC 6 cut(s) 13, 535, 627, 806, 865, 951
AfiI CCNNNNNNNGG 2 cut(s) 196, 860
AgsI TTSAA 5 cut(s) 128, 170, 580, 591, 1004
AhdI GACNNNNNGTC 1 cut(s) 674
AjnI CCWGG 4 cut(s) 83, 244, 953, 1030
AluBI AGCT 7 cut(s) 229, 319, 397, 467, 749, 899, 967
AluI AGCT 7 cut(s) 229, 319, 397, 467, 749, 899, 967
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 1 cut(s) 675
AlwNI CAGNNNCTG 1 cut(s) 899
AoxI GGCC 4 cut(s) 187, 201, 294, 872
ApeKI GCWGC 5 cut(s) 319, 467, 489, 572, 926
ApoI RAATTY 3 cut(s) 620, 795, 1060
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
AspLEI GCGC 2 cut(s) 926, 1071
AspS9I GGNCC 3 cut(s) 188, 202, 295
AsuHPI GGTGA 5 cut(s) 223, 640, 766, 851, 998
AsuII TTCGAA 1 cut(s) 793
BalI TGGCCA 1 cut(s) 874
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 654
BbvI GCAGC 5 cut(s) 306, 454, 501, 559, 913
BccI CCATC 2 cut(s) 338, 983
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 4 cut(s) 85, 246, 955, 1032
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 3 cut(s) 486, 882, 1008
BfmI CTRYAG 1 cut(s) 945
BisI GCNGC 6 cut(s) 187, 320, 468, 490, 573, 927
BlsI GCNGC 6 cut(s) 188, 321, 469, 491, 574, 928
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 4 cut(s) 85, 246, 955, 1032
BmeRI GACNNNNNGTC 1 cut(s) 674
BmgT120I GGNCC 3 cut(s) 188, 202, 295
BmiI GGNNCC 2 cut(s) 403, 478
BmrFI CCNGG 4 cut(s) 85, 246, 955, 1032
BmrI ACTGGG 1 cut(s) 1069
BmsI GCATC 2 cut(s) 317, 484
BmuI ACTGGG 1 cut(s) 1069
Bpu10I CCTNAGC 1 cut(s) 654
Bpu14I TTCGAA 1 cut(s) 793
BpuEI CTTGAG 2 cut(s) 179, 456
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 4 cut(s) 84, 244, 811, 953
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 984, 1014
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 860
Bse1I ACTGG 1 cut(s) 1075
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 4 cut(s) 85, 246, 955, 1032
BseDI CCNNGG 4 cut(s) 84, 244, 811, 953
BseGI GGATG 1 cut(s) 332
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 196, 860
BseMII CTCAG 6 cut(s) 272, 434, 645, 944, 959, 1031
BseNI ACTGG 1 cut(s) 1075
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 5 cut(s) 306, 454, 501, 559, 913
BseYI CCCAGC 2 cut(s) 397, 749
Bsh1236I CGCG 1 cut(s) 186
BshFI GGCC 4 cut(s) 189, 203, 296, 874
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 59, 234
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 2 cut(s) 196, 860
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 4 cut(s) 189, 203, 296, 874
Bsp119I TTCGAA 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 4 cut(s) 423, 680, 691, 1049
BspACI CCGC 3 cut(s) 186, 214, 651
BspANI GGCC 4 cut(s) 189, 203, 296, 874
BspCNI CTCAG 6 cut(s) 273, 433, 646, 943, 960, 1030
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 2 cut(s) 403, 478
BspPI GGATC 1 cut(s) 675
BspT104I TTCGAA 1 cut(s) 793
BsrI ACTGG 1 cut(s) 1075
BssECI CCNNGG 4 cut(s) 84, 244, 811, 953
BssMI GATC 4 cut(s) 423, 680, 691, 1049
BssT1I CCWWGG 1 cut(s) 811
Bst2UI CCWGG 4 cut(s) 85, 246, 955, 1032
Bst4CI ACNGT 2 cut(s) 822, 847
BstBI TTCGAA 1 cut(s) 793
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 8 cut(s) 281, 420, 654, 828, 930, 968, 1017, 1043
BstF5I GGATG 1 cut(s) 332
BstFNI CGCG 1 cut(s) 186
BstHHI GCGC 2 cut(s) 926, 1071
BstKTI GATC 4 cut(s) 426, 683, 694, 1052
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 4 cut(s) 423, 680, 691, 1049
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 4 cut(s) 85, 246, 955, 1032
BstNSI RCATGY 1 cut(s) 220
BstSCI CCNGG 4 cut(s) 83, 244, 953, 1030
BstSFI CTRYAG 1 cut(s) 945
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 5 cut(s) 306, 454, 501, 559, 913
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BsuRI GGCC 4 cut(s) 189, 203, 296, 874
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 1 cut(s) 332
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 768
Cac8I GCNNGC 1 cut(s) 56
CaiI CAGNNNCTG 1 cut(s) 899
CfoI GCGC 2 cut(s) 926, 1071
Cfr13I GGNCC 3 cut(s) 188, 202, 295
CsiI ACCWGGT 1 cut(s) 1030
Csp6I GTAC 6 cut(s) 12, 534, 626, 805, 864, 950
CviAII CATG 3 cut(s) 217, 662, 729
CviQI GTAC 6 cut(s) 12, 534, 626, 805, 864, 950
DdeI CTNAG 8 cut(s) 281, 420, 654, 828, 930, 968, 1017, 1043
DpnI GATC 4 cut(s) 425, 682, 693, 1051
DpnII GATC 4 cut(s) 423, 680, 691, 1049
DriI GACNNNNNGTC 1 cut(s) 674
EaeI YGGCCR 1 cut(s) 872
Eam1105I GACNNNNNGTC 1 cut(s) 674
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 811
Eco24I GRGCYC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 795
EcoRII CCWGG 4 cut(s) 83, 244, 953, 1030
EcoT14I CCWWGG 1 cut(s) 811
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 811
FaeI CATG 3 cut(s) 220, 665, 732
FaqI GGGAC 1 cut(s) 580
FatI CATG 3 cut(s) 216, 661, 728
Fnu4HI GCNGC 6 cut(s) 187, 320, 468, 490, 573, 927
FokI GGATG 1 cut(s) 339
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 6 cut(s) 187, 320, 468, 490, 573, 927
FspBI CTAG 3 cut(s) 486, 882, 1008
GlaI GCGC 2 cut(s) 925, 1070
GluI GCNGC 6 cut(s) 187, 320, 468, 490, 573, 927
GsaI CCCAGC 2 cut(s) 401, 753
HaeIII GGCC 4 cut(s) 189, 203, 296, 874
HapII CCGG 2 cut(s) 59, 234
HhaI GCGC 2 cut(s) 926, 1071
Hin1II CATG 3 cut(s) 220, 665, 732
Hin6I GCGC 2 cut(s) 924, 1069
HinP1I GCGC 2 cut(s) 924, 1069
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 5 cut(s) 166, 334, 389, 517, 1004
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 2 cut(s) 59, 234
HphI GGTGA 5 cut(s) 223, 640, 766, 851, 998
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 8 cut(s) 165, 226, 258, 282, 382, 423, 680, 933
Hpy188III TCNNGA 1 cut(s) 614
Hpy8I GTNNAC 1 cut(s) 526
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 6 cut(s) 595, 655, 694, 735, 751, 829
HpyCH4III ACNGT 2 cut(s) 822, 847
HpyCH4V TGCA 3 cut(s) 330, 460, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpyF3I CTNAG 8 cut(s) 281, 420, 654, 828, 930, 968, 1017, 1043
Hsp92II CATG 3 cut(s) 220, 665, 732
HspAI GCGC 2 cut(s) 924, 1069
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 4 cut(s) 423, 680, 691, 1049
LmnI GCTCC 2 cut(s) 226, 316
Lsp1109I GCAGC 5 cut(s) 306, 454, 501, 559, 913
LweI GCATC 2 cut(s) 317, 484
MabI ACCWGGT 1 cut(s) 1030
MaeI CTAG 3 cut(s) 486, 882, 1008
MaeIII GTNAC 1 cut(s) 740
MalI GATC 4 cut(s) 425, 682, 693, 1051
MboI GATC 4 cut(s) 423, 680, 691, 1049
MboII GAAGA 4 cut(s) 269, 592, 844, 917
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 874
MluCI AATT 7 cut(s) 7, 206, 557, 620, 795, 981, 1060
MluNI TGGCCA 1 cut(s) 874
MmeI TCCRAC 3 cut(s) 236, 294, 646
Mox20I TGGCCA 1 cut(s) 874
MscI TGGCCA 1 cut(s) 874
MseI TTAA 2 cut(s) 501, 525
Msp20I TGGCCA 1 cut(s) 874
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 2 cut(s) 59, 234
MspR9I CCNGG 4 cut(s) 85, 246, 955, 1032
MvaI CCWGG 4 cut(s) 85, 246, 955, 1032
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 425
NdeII GATC 4 cut(s) 423, 680, 691, 1049
NlaIII CATG 3 cut(s) 220, 665, 732
NlaIV GGNNCC 2 cut(s) 403, 478
NspI RCATGY 1 cut(s) 220
NspV TTCGAA 1 cut(s) 793
PcsI WCGNNNNNNNCGW 1 cut(s) 1054
PfeI GAWTC 5 cut(s) 166, 334, 389, 517, 1004
PflMI CCANNNNNTGG 1 cut(s) 860
PkrI GCNGC 6 cut(s) 188, 321, 469, 491, 574, 928
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 4 cut(s) 83, 244, 953, 1030
PspFI CCCAGC 2 cut(s) 397, 749
PspGI CCWGG 4 cut(s) 83, 244, 953, 1030
PspN4I GGNNCC 2 cut(s) 403, 478
PspPI GGNCC 3 cut(s) 188, 202, 295
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 1 cut(s) 680
RsaI GTAC 6 cut(s) 13, 535, 627, 806, 865, 951
RsaNI GTAC 6 cut(s) 12, 534, 626, 805, 864, 950
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 501, 525
SatI GCNGC 6 cut(s) 187, 320, 468, 490, 573, 927
Sau3AI GATC 4 cut(s) 423, 680, 691, 1049
Sau96I GGNCC 3 cut(s) 188, 202, 295
ScaI AGTACT 1 cut(s) 806
ScrFI CCNGG 4 cut(s) 85, 246, 955, 1032
SduI GDGCHC 1 cut(s) 231
SexAI ACCWGGT 1 cut(s) 1030
SfaNI GCATC 2 cut(s) 317, 484
SfcI CTRYAG 1 cut(s) 945
SfuI TTCGAA 1 cut(s) 793
SmlI CTYRAG 2 cut(s) 194, 471
SmoI CTYRAG 2 cut(s) 194, 471
Sse9I AATT 7 cut(s) 7, 206, 557, 620, 795, 981, 1060
SsiI CCGC 3 cut(s) 186, 214, 651
SspMI CTAG 3 cut(s) 486, 882, 1008
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 4 cut(s) 83, 244, 953, 1030
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 2 cut(s) 822, 847
TaqI TCGA 2 cut(s) 793, 1057
TasI AATT 7 cut(s) 7, 206, 557, 620, 795, 981, 1060
TatI WGTACW 2 cut(s) 11, 804
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 5 cut(s) 166, 334, 389, 517, 1004
Tru1I TTAA 2 cut(s) 501, 525
Tru9I TTAA 2 cut(s) 501, 525
TscAI CASTG 1 cut(s) 768
TseI GCWGC 5 cut(s) 319, 467, 489, 572, 926
TspDTI ATGAA 4 cut(s) 551, 633, 717, 788
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 768
Van91I CCANNNNNTGG 1 cut(s) 860
XapI RAATTY 3 cut(s) 620, 795, 1060
XceI RCATGY 1 cut(s) 220
XspI CTAG 3 cut(s) 486, 882, 1008
ZrmI AGTACT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.