Rh5CG405300

Non-structural maintenance of chromosomes element 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003611
Physical Location & Seq
Reverse (-)
54212796 .. 54218166
5371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG405300.1

Sequence Viewer

Length: 669 bp
ATGACGGAACTGAACGCCAAGCACCACACTCTGATTCAAGCTCTGCTCTCTCGCGGCCCACTTAAAGAGGGCCAGTTCCACCGCATGTTCTCGGAACTCACCGGAGAAAACCCAGGTGTTCGTCGGAAGGCGTTTGATGATTATCTTCTGAGGATAAACAAGGCCCTTTCTTATGTTCAGTTCGAGCTGCGGGGATGCAGGAATCAATATGATGGTCAAGTTTATTATGGAGTTGTCAATAATGTCTCTGATGAAGAATCGAAGCTGGGAACGACTTATTCGGTTGCTCAGATTGCTTTCTATAAGGCTATTATAGAAGCAATAGTGCAAGAAGCTGCTCAAGGAACCATATCTAGCAGTGATGCTCTTAATCTACGATTAGAGAATCAGGTTAACAGTGGTACATCATCACAATCACAAGGAGGTCCAGTTCCTATCCCTACTGCATTGAAGAACTTTTCAATGTCCCAGAAGGAAAAAACTCTTGATGAATTTGTACGGGATAAATGGCTTTCCTTCACTGCTGAGGGTCATGTTGGACTTGGTGTCAGATCCTTCCTTGATCTGCGAAGTTGGTTTCGCAATAATGATGTTCCTTCATGCGAAGTGTGCAATGAAGCTGGTGTGAAGGTTGTCTCTTGCTTTGTCTCACAATTGTTCAATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMC_Nse1 zf-RING-like

Protein Analysis

222

Amino Acids

24.76

Weight (kDa)

6.44

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_Nse1 PF07574 8 - 189 5.8e-47 Nse1 non-SMC component of SMC5-6 complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 54
AciI CCGC 3 cut(s) 54, 82, 190
AclWI GGATC 1 cut(s) 546
AcsI RAATTY 1 cut(s) 491
AfaI GTAC 2 cut(s) 403, 498
AgsI TTSAA 4 cut(s) 38, 451, 462, 661
AhdI GACNNNNNGTC 1 cut(s) 545
AjnI CCWGG 1 cut(s) 112
AloI GAACNNNNNNTCC 2 cut(s) 414, 446
AluBI AGCT 5 cut(s) 41, 187, 265, 335, 620
AluI AGCT 5 cut(s) 41, 187, 265, 335, 620
Alw26I GTCTC 3 cut(s) 250, 640, 652
AlwI GGATC 1 cut(s) 546
AoxI GGCC 3 cut(s) 55, 70, 162
ApeKI GCWGC 2 cut(s) 187, 335
ApoI RAATTY 1 cut(s) 491
AspS9I GGNCC 4 cut(s) 56, 70, 163, 425
AsuHPI GGTGA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 174, 322
BccI CCATC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 114
BcoDI GTCTC 3 cut(s) 250, 640, 652
BfaI CTAG 1 cut(s) 354
BisI GCNGC 3 cut(s) 55, 188, 336
BlsI GCNGC 3 cut(s) 56, 189, 337
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 425
BmeRI GACNNNNNGTC 1 cut(s) 545
BmgT120I GGNCC 4 cut(s) 56, 70, 163, 425
BmiI GGNNCC 1 cut(s) 346
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 185, 352
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bse1I ACTGG 2 cut(s) 73, 428
Bse3DI GCAATG 1 cut(s) 619
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 200
BseJI GATNNNNATC 1 cut(s) 141
BseMI GCAATG 1 cut(s) 619
BseMII CTCAG 3 cut(s) 140, 302, 516
BseNI ACTGG 2 cut(s) 73, 428
BseXI GCAGC 2 cut(s) 174, 322
BseYI CCCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 57, 72, 164
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 451
BsmAI GTCTC 3 cut(s) 250, 640, 652
BsmFI GGGAC 1 cut(s) 451
BsnI GGCC 3 cut(s) 57, 72, 164
Bsp143I GATC 2 cut(s) 551, 562
BspACI CCGC 3 cut(s) 54, 82, 190
BspANI GGCC 3 cut(s) 57, 72, 164
BspCNI CTCAG 3 cut(s) 141, 301, 517
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 346
BspPI GGATC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 619
BsrI ACTGG 2 cut(s) 73, 428
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 2 cut(s) 551, 562
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 398
BstDEI CTNAG 3 cut(s) 149, 288, 525
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 2 cut(s) 554, 565
BstMAI GTCTC 3 cut(s) 250, 640, 652
BstMBI GATC 2 cut(s) 551, 562
BstMWI GCNNNNNNNGC 2 cut(s) 293, 609
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 112
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 174, 322
BstX2I RGATCY 1 cut(s) 551
BstYI RGATCY 1 cut(s) 551
BsuRI GGCC 3 cut(s) 57, 72, 164
BtsCI GGATG 1 cut(s) 200
BtsI GCAGTG 2 cut(s) 364, 519
BtsIMutI CAGTG 3 cut(s) 364, 403, 519
Cfr13I GGNCC 4 cut(s) 56, 70, 163, 425
Csp6I GTAC 2 cut(s) 402, 497
CviAII CATG 3 cut(s) 85, 533, 600
CviQI GTAC 2 cut(s) 402, 497
DdeI CTNAG 3 cut(s) 149, 288, 525
DpnI GATC 2 cut(s) 553, 564
DpnII GATC 2 cut(s) 551, 562
DriI GACNNNNNGTC 1 cut(s) 545
Eam1105I GACNNNNNGTC 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 3 cut(s) 88, 536, 603
FaqI GGGAC 1 cut(s) 451
FatI CATG 3 cut(s) 84, 532, 599
FauI CCCGC 1 cut(s) 183
Fnu4HI GCNGC 3 cut(s) 55, 188, 336
FokI GGATG 1 cut(s) 207
Fsp4HI GCNGC 3 cut(s) 55, 188, 336
FspBI CTAG 1 cut(s) 354
GluI GCNGC 3 cut(s) 55, 188, 336
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 3 cut(s) 57, 72, 164
HapII CCGG 1 cut(s) 102
Hin1II CATG 3 cut(s) 88, 536, 603
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 4 cut(s) 34, 202, 257, 385
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 1 cut(s) 102
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 7 cut(s) 33, 94, 126, 150, 250, 291, 551
Hpy188III TCNNGA 1 cut(s) 485
Hpy8I GTNNAC 1 cut(s) 394
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 6 cut(s) 121, 466, 526, 565, 606, 622
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4V TGCA 4 cut(s) 198, 328, 446, 612
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 609
HpyF3I CTNAG 3 cut(s) 149, 288, 525
Hsp92II CATG 3 cut(s) 88, 536, 603
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 551, 562
Lsp1109I GCAGC 2 cut(s) 174, 322
LweI GCATC 2 cut(s) 185, 352
MaeI CTAG 1 cut(s) 354
MalI GATC 2 cut(s) 553, 564
MboI GATC 2 cut(s) 551, 562
MboII GAAGA 3 cut(s) 137, 266, 463
MfeI CAATTG 1 cut(s) 653
MflI RGATCY 1 cut(s) 551
MluCI AATT 3 cut(s) 491, 653, 661
MmeI TCCRAC 2 cut(s) 104, 517
MnlI CCTC 4 cut(s) 61, 144, 416, 520
MseI TTAA 3 cut(s) 63, 369, 393
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 1 cut(s) 114
MunI CAATTG 1 cut(s) 653
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 293, 609
NdeII GATC 2 cut(s) 551, 562
NlaIII CATG 3 cut(s) 88, 536, 603
NlaIV GGNNCC 1 cut(s) 346
NspI RCATGY 1 cut(s) 88
PfeI GAWTC 4 cut(s) 34, 202, 257, 385
PkrI GCNGC 3 cut(s) 56, 189, 337
Psp6I CCWGG 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 346
PspPI GGNCC 4 cut(s) 56, 70, 163, 425
PsuI RGATCY 1 cut(s) 551
RsaI GTAC 2 cut(s) 403, 498
RsaNI GTAC 2 cut(s) 402, 497
SaqAI TTAA 3 cut(s) 63, 369, 393
SatI GCNGC 3 cut(s) 55, 188, 336
Sau3AI GATC 2 cut(s) 551, 562
Sau96I GGNCC 4 cut(s) 56, 70, 163, 425
ScrFI CCNGG 1 cut(s) 114
SetI ASST 9 cut(s) 43, 118, 189, 267, 337, 393, 427, 622, 633
SfaNI GCATC 2 cut(s) 185, 352
SinI GGWCC 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 339
SmoI CTYRAG 1 cut(s) 339
Sse9I AATT 3 cut(s) 491, 653, 661
SsiI CCGC 3 cut(s) 54, 82, 190
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 398
TaqI TCGA 2 cut(s) 183, 260
TasI AATT 3 cut(s) 491, 653, 661
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 4 cut(s) 34, 202, 257, 385
Tru1I TTAA 3 cut(s) 63, 369, 393
Tru9I TTAA 3 cut(s) 63, 369, 393
TscAI CASTG 3 cut(s) 364, 403, 526
TseI GCWGC 2 cut(s) 187, 335
TspDTI ATGAA 5 cut(s) 267, 504, 588, 630, 654
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 3 cut(s) 364, 403, 526
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 1 cut(s) 491
XceI RCATGY 1 cut(s) 88
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.