RLG00000035103

Non-structural maintenance of chromosomes element 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003611
Physical Location & Seq
Reverse (-)
60915597 .. 60920915
5319 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035103

Sequence Viewer

Length: 456 bp
ATGACGGAACTGAACGCCAAGCACCACACTCTGATTCAAGCTCTGCTCTCTCGCGGCCCACTCAAAGAGGACCAGTTCCACCGCATGTTCTCGGAACTCACCGGAGAAAACCCAGGTGTTCGTCGGAAGGCGTTTGATGATTATCTTCTGAGGATAAACAAGGCACTTTCTTATGTTCAGTTCGAGTTGCGGGGATGCAGGAATCAATATGATGGTCAAGTTTATTATGGAGTTGTTAATAATGTTTCTGATGAAGAATCAAAGCTGGGAACGACTTATTCGGTTGCTCAGATTGCTTTCTATAAGGCTATTGAGCGGAGCCATGGCATAACTAATGACTCAAATCCTGCTGAGCATCACTACTTCGATCAATTCAGCCATGAGATCTCTAACACTCCAATGCGAGTTGAGGAGCACCGAGTAATGGGTCATTACGGATCAAAATTGGTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMC_Nse1 zf-RING-like

Protein Analysis

152

Amino Acids

17.41

Weight (kDa)

6.49

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_Nse1 PF07574 8 - 104 1.2e-28 Nse1 non-SMC component of SMC5-6 complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 316
AccII CGCG 1 cut(s) 54
AciI CCGC 4 cut(s) 54, 82, 190, 316
AclWI GGATC 1 cut(s) 445
AfiI CCNNNNNNNGG 1 cut(s) 424
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 2 cut(s) 41, 265
AluI AGCT 2 cut(s) 41, 265
Alw21I GWGCWC 1 cut(s) 417
AlwI GGATC 1 cut(s) 445
AoxI GGCC 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 56, 70
AsuHPI GGTGA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 417
BccI CCATC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 114
BglII AGATCT 1 cut(s) 384
BisI GCNGC 1 cut(s) 55
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 56, 70
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 185, 364
Bpu1102I GCTNAGC 1 cut(s) 351
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 112, 322
BsaWI WCCGGW 1 cut(s) 101
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 112, 322
BseGI GGATG 1 cut(s) 200
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMII CTCAG 3 cut(s) 140, 302, 342
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 425
BseYI CCCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 424
BsnI GGCC 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 3 cut(s) 367, 384, 437
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 322
BspACI CCGC 4 cut(s) 54, 82, 190, 316
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 3 cut(s) 141, 301, 343
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 320
BspPI GGATC 1 cut(s) 445
BsrBI CCGCTC 1 cut(s) 316
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 112, 322
BssMI GATC 3 cut(s) 367, 384, 437
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 114
BstDEI CTNAG 3 cut(s) 149, 288, 351
BstDSI CCRYGG 1 cut(s) 322
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 3 cut(s) 370, 387, 440
BstMBI GATC 3 cut(s) 367, 384, 437
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 112
BstUI CGCG 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
BsuRI GGCC 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 322
BtsCI GGATG 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 56, 70
CspCI CAANNNNNGTGG 1 cut(s) 429
CviAII CATG 4 cut(s) 85, 323, 380, 453
CviJI RGCY 6 cut(s) 41, 57, 265, 308, 321, 378
CviKI_1 RGCY 6 cut(s) 41, 57, 265, 308, 321, 378
DdeI CTNAG 3 cut(s) 149, 288, 351
DpnI GATC 3 cut(s) 369, 386, 439
DpnII GATC 3 cut(s) 367, 384, 437
Eco130I CCWWGG 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 4 cut(s) 88, 326, 383, 456
FaiI YATR 9 cut(s) 86, 174, 210, 228, 303, 324, 329, 381, 454
FatI CATG 4 cut(s) 84, 322, 379, 452
FauI CCCGC 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 55
FokI GGATG 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 55
GluI GCNGC 1 cut(s) 55
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 1 cut(s) 57
HapII CCGG 1 cut(s) 102
Hin1II CATG 4 cut(s) 88, 326, 383, 456
HinfI GANTC 4 cut(s) 34, 202, 257, 338
HpaII CCGG 1 cut(s) 102
HphI GGTGA 1 cut(s) 91
Hpy188I TCNGA 6 cut(s) 33, 94, 126, 150, 250, 291
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 1 cut(s) 121
HpyCH4V TGCA 1 cut(s) 198
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 3 cut(s) 149, 288, 351
Hsp92II CATG 4 cut(s) 88, 326, 383, 456
Kzo9I GATC 3 cut(s) 367, 384, 437
LmnI GCTCC 2 cut(s) 318, 412
LpnPI CCDG 7 cut(s) 86, 99, 115, 126, 184, 251, 360
LweI GCATC 2 cut(s) 185, 364
MalI GATC 3 cut(s) 369, 386, 439
MbiI CCGCTC 1 cut(s) 316
MboI GATC 3 cut(s) 367, 384, 437
MboII GAAGA 2 cut(s) 137, 266
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 2 cut(s) 371, 443
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 3 cut(s) 61, 144, 403
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 293
NcoI CCATGG 1 cut(s) 322
NdeII GATC 3 cut(s) 367, 384, 437
NlaIII CATG 4 cut(s) 88, 326, 383, 456
NlaIV GGNNCC 1 cut(s) 320
NspI RCATGY 1 cut(s) 88
PfeI GAWTC 3 cut(s) 34, 202, 257
PkrI GCNGC 1 cut(s) 56
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 320
PspPI GGNCC 2 cut(s) 56, 70
PsuI RGATCY 1 cut(s) 384
RseI CAYNNNNRTG 1 cut(s) 398
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 1 cut(s) 55
Sau3AI GATC 3 cut(s) 367, 384, 437
Sau96I GGNCC 2 cut(s) 56, 70
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 417
SetI ASST 3 cut(s) 43, 118, 267
SfaNI GCATC 2 cut(s) 185, 364
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 398
Sse9I AATT 2 cut(s) 371, 443
SsiI CCGC 4 cut(s) 54, 82, 190, 316
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 1 cut(s) 322
TaqI TCGA 2 cut(s) 183, 366
TasI AATT 2 cut(s) 371, 443
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 3 cut(s) 34, 202, 257
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TspDTI ATGAA 1 cut(s) 267
TspGWI ACGGA 2 cut(s) 20, 450
VpaK11BI GGWCC 1 cut(s) 70
XceI RCATGY 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.