FvH4_3g33520

Phosphatidylinositol transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008855
Physical Location & Seq
Forward (+)
28942313 .. 28948085
5773 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33520.t1

Sequence Viewer

Length: 882 bp
ATGGTTCTGATGAAGGAATTTCGTATTGTGATGCCCATGTCTCTGGAAGAGTATCAAGTTGCTCAGGCATATATGGTCACAAAGATGCAGCAACAGAACACGAATAGTACGGAAGGGGTCGAAGTTTTAGAGAATAAATCCTTTGAAGATGATGTACTTGGAAAGGGTCAATACACGTCTAAAGTATACCGTCTGCAAAGCAAAGCTCCTACTTGGCTTACCACCTTTGCACCAGCAGATGCACTCATCATGCAAGAGGAAGCCTGGAACGCATACCCAAGATGCAAAACAGTAATCAAGTGCCCATACTTCACCAAATTTCAGTTGACCATTGAAACTGTTCATATGGCTGACAATGGCCAGTCCGAAAATGTACATGGTCTAAGCAAAGAAAAGCTAGCAGCCAGGGATGTGGAAATCGTTGATATAGCTTCAGCTGCAAGGGATTACTGGAGTTATGTGGTTGGTAGTAACAATACAGACTTTTCCATTTTTAAATCAGAGAAGACTGGCCGTGGTCCACTTTTAGAGGGATGGCAGGATATGTCAAGTCCAGTTATGACAGCCTACAAGTTGGTCACCATAGATGCACCATATTGGGGATTTGGCTATAAACTTGAACAAGCTTTGCTATTGGGTGAAAGGGCCCTTTTCCTTGAAAGCCATCGAAATTGTTTTGCTTGGATAGATGAATGGTTTGGAATGACTATGCAACAGCTACGTGAACTTGATCAAGAAAGCGACTTGTTAGCTGAGGTGCAGAAACATGGGATGTCAACTTTGGTGAAGAGCTTGGACGATTTTGGCCAAAGAATAACACCTGAAAGTGAAGACATTTATCGTGGCCAGAGGTCTCAACTCTCGACTCAACTATCAACTTAA

Protein Analysis

294

Amino Acids

33.52

Weight (kDa)

4.97

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IP_trans PF02121 2 - 246 5.7e-95 Phosphatidylinositol transfer protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AcoI YGGCCR 4 cut(s) 358, 511, 805, 844
AcsI RAATTY 2 cut(s) 17, 317
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 3 cut(s) 109, 156, 375
AfiI CCNNNNNNNGG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 4 cut(s) 146, 335, 620, 659
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 2 cut(s) 263, 404
AluBI AGCT 8 cut(s) 206, 397, 431, 437, 626, 718, 752, 792
AluI AGCT 8 cut(s) 206, 397, 431, 437, 626, 718, 752, 792
Alw26I GTCTC 2 cut(s) 45, 858
AoxI GGCC 5 cut(s) 358, 511, 645, 805, 844
ApaI GGGCCC 1 cut(s) 649
ApeKI GCWGC 3 cut(s) 88, 401, 437
ApoI RAATTY 2 cut(s) 17, 317
Asp700I GAANNNNTTC 1 cut(s) 339
AspS9I GGNCC 3 cut(s) 518, 645, 646
AsuHPI GGTGA 4 cut(s) 304, 571, 650, 796
AsuNHI GCTAGC 1 cut(s) 397
AvaII GGWCC 1 cut(s) 518
BaeGI GKGCMC 2 cut(s) 305, 649
BalI TGGCCA 3 cut(s) 360, 807, 846
BanII GRGCYC 1 cut(s) 649
BarI GAAGNNNNNNTAC 2 cut(s) 138, 170
BbsI GAAGAC 2 cut(s) 512, 837
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 3 cut(s) 100, 413, 424
BccI CCATC 2 cut(s) 528, 672
BceAI ACGGC 1 cut(s) 498
BciT130I CCWGG 2 cut(s) 265, 406
BclI TGATCA 1 cut(s) 730
BcoDI GTCTC 2 cut(s) 45, 858
BfaI CTAG 1 cut(s) 398
BisI GCNGC 3 cut(s) 89, 402, 438
BlsI GCNGC 3 cut(s) 90, 403, 439
Bme1390I CCNGG 2 cut(s) 265, 406
Bme18I GGWCC 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 3 cut(s) 518, 645, 646
BmiI GGNNCC 1 cut(s) 647
BmrFI CCNGG 2 cut(s) 265, 406
BmsI GCATC 5 cut(s) 21, 75, 229, 272, 577
BmtI GCTAGC 1 cut(s) 401
BpiI GAAGAC 2 cut(s) 512, 837
BpmI CTGGAG 1 cut(s) 472
Bpu10I CCTNAGC 2 cut(s) 63, 753
BsaAI YACGTR 1 cut(s) 722
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 2 cut(s) 405, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse1I ACTGG 4 cut(s) 361, 455, 514, 554
BseBI CCWGG 2 cut(s) 265, 406
BseDI CCNNGG 2 cut(s) 405, 514
BseGI GGATG 3 cut(s) 415, 539, 777
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMII CTCAG 2 cut(s) 77, 744
BseNI ACTGG 4 cut(s) 361, 455, 514, 554
BseSI GKGCMC 2 cut(s) 305, 649
BseXI GCAGC 3 cut(s) 100, 413, 424
BsgI GTGCAG 1 cut(s) 779
BshFI GGCC 5 cut(s) 360, 513, 647, 807, 846
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 2 cut(s) 45, 858
BsnI GGCC 5 cut(s) 360, 513, 647, 807, 846
Bso31I GGTCTC 1 cut(s) 858
Bsp120I GGGCCC 1 cut(s) 645
Bsp1286I GDGCHC 2 cut(s) 305, 649
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 1 cut(s) 730
BspANI GGCC 5 cut(s) 360, 513, 647, 807, 846
BspCNI CTCAG 2 cut(s) 76, 745
BspLI GGNNCC 1 cut(s) 647
BspOI GCTAGC 1 cut(s) 401
BspQI GCTCTTC 1 cut(s) 782
BspTNI GGTCTC 1 cut(s) 858
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 4 cut(s) 361, 455, 514, 554
BssECI CCNNGG 2 cut(s) 405, 514
BssMI GATC 1 cut(s) 730
BssNAI GTATAC 1 cut(s) 187
Bst1107I GTATAC 1 cut(s) 187
Bst2UI CCWGG 2 cut(s) 265, 406
Bst4CI ACNGT 3 cut(s) 191, 292, 340
Bst6I CTCTTC 2 cut(s) 42, 782
BstAUI TGTACA 1 cut(s) 373
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 3 cut(s) 63, 383, 753
BstDSI CCRYGG 1 cut(s) 514
BstEII GGTNACC 1 cut(s) 577
BstF5I GGATG 3 cut(s) 415, 539, 777
BstKTI GATC 1 cut(s) 733
BstMAI GTCTC 2 cut(s) 45, 858
BstMBI GATC 1 cut(s) 730
BstMWI GCNNNNNNNGC 2 cut(s) 269, 437
BstNI CCWGG 2 cut(s) 265, 406
BstPI GGTNACC 1 cut(s) 577
BstSCI CCNGG 2 cut(s) 263, 404
BstSLI GKGCMC 2 cut(s) 305, 649
BstV1I GCAGC 3 cut(s) 100, 413, 424
BstV2I GAAGAC 2 cut(s) 512, 837
BstXI CCANNNNNNTGG 2 cut(s) 43, 412
BstZ17I GTATAC 1 cut(s) 187
BsuRI GGCC 5 cut(s) 360, 513, 647, 807, 846
BtgI CCRYGG 1 cut(s) 514
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 3 cut(s) 415, 539, 777
Cac8I GCNNGC 1 cut(s) 399
Cfr13I GGNCC 3 cut(s) 518, 645, 646
Csp6I GTAC 3 cut(s) 108, 155, 374
CviAII CATG 4 cut(s) 37, 250, 377, 767
CviQI GTAC 3 cut(s) 108, 155, 374
DdeI CTNAG 3 cut(s) 63, 383, 753
DpnI GATC 1 cut(s) 732
DpnII GATC 1 cut(s) 730
DraI TTTAAA 1 cut(s) 496
EaeI YGGCCR 4 cut(s) 358, 511, 805, 844
Eam1104I CTCTTC 2 cut(s) 42, 782
EarI CTCTTC 2 cut(s) 42, 782
Eco24I GRGCYC 1 cut(s) 649
Eco31I GGTCTC 1 cut(s) 858
Eco47I GGWCC 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 417
Eco91I GGTNACC 1 cut(s) 577
EcoO109I RGGNCCY 2 cut(s) 645, 646
EcoO65I GGTNACC 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 263, 404
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 4 cut(s) 40, 253, 380, 770
FatI CATG 4 cut(s) 36, 249, 376, 766
FauNDI CATATG 1 cut(s) 345
FbaI TGATCA 1 cut(s) 730
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 89, 402, 438
FokI GGATG 3 cut(s) 422, 546, 784
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 3 cut(s) 89, 402, 438
FspBI CTAG 1 cut(s) 398
GluI GCNGC 3 cut(s) 89, 402, 438
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 5 cut(s) 360, 513, 647, 807, 846
Hin1II CATG 4 cut(s) 40, 253, 380, 770
HincII GTYRAC 2 cut(s) 327, 777
HindII GTYRAC 2 cut(s) 327, 777
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 1 cut(s) 865
HphI GGTGA 4 cut(s) 304, 571, 650, 796
Hpy166II GTNNAC 5 cut(s) 187, 327, 521, 725, 777
Hpy188I TCNGA 3 cut(s) 9, 367, 502
Hpy188III TCNNGA 3 cut(s) 44, 734, 862
Hpy8I GTNNAC 5 cut(s) 187, 327, 521, 725, 777
HpyAV CCTTC 2 cut(s) 7, 107
HpyCH4III ACNGT 3 cut(s) 191, 292, 340
HpyCH4IV ACGT 2 cut(s) 176, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 437
HpyF3I CTNAG 3 cut(s) 63, 383, 753
HpySE526I ACGT 2 cut(s) 176, 721
Hsp92II CATG 4 cut(s) 40, 253, 380, 770
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 1 cut(s) 730
LguI GCTCTTC 1 cut(s) 782
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 3 cut(s) 100, 413, 424
LweI GCATC 5 cut(s) 21, 75, 229, 272, 577
MaeI CTAG 1 cut(s) 398
MaeII ACGT 2 cut(s) 176, 721
MaeIII GTNAC 3 cut(s) 76, 470, 577
MalI GATC 1 cut(s) 732
MboI GATC 1 cut(s) 730
MboII GAAGA 5 cut(s) 59, 158, 517, 799, 842
MhlI GDGCHC 2 cut(s) 305, 649
MlsI TGGCCA 3 cut(s) 360, 807, 846
MluCI AATT 3 cut(s) 17, 317, 670
MluNI TGGCCA 3 cut(s) 360, 807, 846
MlyI GAGTC 1 cut(s) 859
MnlI CCTC 4 cut(s) 250, 523, 748, 843
Mox20I TGGCCA 3 cut(s) 360, 807, 846
MroXI GAANNNNTTC 1 cut(s) 339
MscI TGGCCA 3 cut(s) 360, 807, 846
MseI TTAA 2 cut(s) 495, 880
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 3 cut(s) 360, 807, 846
MspA1I CMGCKG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 265, 406
MvaI CCWGG 2 cut(s) 265, 406
MwoI GCNNNNNNNGC 2 cut(s) 269, 437
NdeI CATATG 1 cut(s) 345
NdeII GATC 1 cut(s) 730
NheI GCTAGC 1 cut(s) 397
NlaIII CATG 4 cut(s) 40, 253, 380, 770
NlaIV GGNNCC 1 cut(s) 647
NmuCI GTSAC 2 cut(s) 76, 577
PciSI GCTCTTC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 339
PkrI GCNGC 3 cut(s) 90, 403, 439
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
Ppu21I YACGTR 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 263, 404
PspEI GGTNACC 1 cut(s) 577
PspGI CCWGG 2 cut(s) 263, 404
PspN4I GGNNCC 1 cut(s) 647
PspOMI GGGCCC 1 cut(s) 645
PspPI GGNCC 3 cut(s) 518, 645, 646
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 3 cut(s) 109, 156, 375
RsaNI GTAC 3 cut(s) 108, 155, 374
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 782
SaqAI TTAA 2 cut(s) 495, 880
SatI GCNGC 3 cut(s) 89, 402, 438
Sau3AI GATC 1 cut(s) 730
Sau96I GGNCC 3 cut(s) 518, 645, 646
SchI GAGTC 1 cut(s) 859
ScrFI CCNGG 2 cut(s) 265, 406
SduI GDGCHC 2 cut(s) 305, 649
SfaNI GCATC 5 cut(s) 21, 75, 229, 272, 577
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 3 cut(s) 17, 317, 670
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 2 cut(s) 263, 404
TaaI ACNGT 3 cut(s) 191, 292, 340
TaiI ACGT 2 cut(s) 179, 724
TaqI TCGA 3 cut(s) 120, 667, 863
TasI AATT 3 cut(s) 17, 317, 670
TatI WGTACW 2 cut(s) 154, 373
Tru1I TTAA 2 cut(s) 495, 880
Tru9I TTAA 2 cut(s) 495, 880
TseFI GTSAC 2 cut(s) 76, 577
TseI GCWGC 3 cut(s) 88, 401, 437
Tsp45I GTSAC 2 cut(s) 76, 577
TspDTI ATGAA 3 cut(s) 26, 332, 705
TspGWI ACGGA 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 2 cut(s) 17, 317
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.