RLG00000035418

Phosphatidylinositol transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008855
Physical Location & Seq
Forward (+)
66713485 .. 66717452
3968 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035418

Sequence Viewer

Length: 873 bp
ATGAAGGAATTTCGTATTGTGATGCCCATGTCTCTAGAAGAGTATCAAGTTGCTCAGGCATATATGGTCACAAAGATGCAGCAACAGAACACAAATAGTACGGAAGGGGTGGAAATTTTAGAGAATAAATCCTTTGAAGATGATGTATTTGGAAAGGGTCAATACACTTCTAAGGTTTATCGTCTACAAAGCAAAGCTCCTACTTGGCTTACAACTTTTGCACCAGCAGATGCACTCATCATGCAAGAGGAAGCCTGGAATGCATACCCAAGATGCAAAACAGTAATCAAGTGCCCATACTTCACCAAATTTCAGTTGACCATTGAAACTGTTCATAGGGCTGACAATGGCCAGTCCGAAAATGTACATGGTCTAAGCAAAGAAATATTAGCAGCCAGGGATGTGGAAGTTGTTGATATAGCTTCATCTGCAAGGGATTACTGGAGTTACGTGGTTGGTAGTAACAATACAGACTTCTCTAATTTTAAATCAGAGAAGACTGGCCGTGGTCCACTTTTAGAGGGATGGCAGGATACATCAAGTCCAGTTATGACAGCCTACAAGTTGGTCACCATAGATGCGCCGTATTGGGGATTTGGCTATAAACTTGAACAAGCTTTGCTATTGGGTGAAAGGGCTCTTTTCCTTGAAAGTCATCGCAATTGTTTTGCTTGGATAGATGAATGGTTTGGAATGACCATGCAACAGCTACGTGAACTTGATCAACAAAGCGACTCATTAGCTGAGCTGCAGAAACATGGGAGGCCAACTTTGGTGAAGAGCATGGACGATTTTGGCCAGAGATTATCAACTGAAAATGAAGACATTTATCTTGGCCAGAGGTCTCAACTCTCGACTCAACTATCAACTTAA

Protein Analysis

291

Amino Acids

33.22

Weight (kDa)

5.02

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IP_trans PF02121 1 - 243 1.1e-93 Phosphatidylinositol transfer protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 184
AcoI YGGCCR 4 cut(s) 349, 502, 796, 835
AcsI RAATTY 3 cut(s) 8, 114, 308
AfaI GTAC 2 cut(s) 100, 366
AfiI CCNNNNNNNGG 1 cut(s) 590
AgsI TTSAA 4 cut(s) 137, 326, 611, 650
AjnI CCWGG 2 cut(s) 254, 395
AluBI AGCT 6 cut(s) 197, 422, 617, 709, 743, 748
AluI AGCT 6 cut(s) 197, 422, 617, 709, 743, 748
Alw26I GTCTC 2 cut(s) 36, 849
AoxI GGCC 5 cut(s) 349, 502, 764, 796, 835
ApeKI GCWGC 3 cut(s) 79, 392, 748
ApoI RAATTY 3 cut(s) 8, 114, 308
Asp700I GAANNNNTTC 1 cut(s) 330
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 1 cut(s) 509
AsuHPI GGTGA 4 cut(s) 295, 562, 641, 787
AvaII GGWCC 1 cut(s) 509
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 3 cut(s) 351, 798, 837
BanII GRGCYC 1 cut(s) 640
BbsI GAAGAC 2 cut(s) 503, 828
BbvI GCAGC 3 cut(s) 91, 404, 735
BccI CCATC 1 cut(s) 519
BceAI ACGGC 2 cut(s) 489, 568
BciT130I CCWGG 2 cut(s) 256, 397
BciVI GTATCC 1 cut(s) 526
BclI TGATCA 1 cut(s) 721
BcoDI GTCTC 2 cut(s) 36, 849
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 1 cut(s) 749
BfuI GTATCC 1 cut(s) 526
BisI GCNGC 3 cut(s) 80, 393, 749
BlpI GCTNAGC 1 cut(s) 744
BlsI GCNGC 3 cut(s) 81, 394, 750
Bme1390I CCNGG 2 cut(s) 256, 397
Bme18I GGWCC 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 509
BmrFI CCNGG 2 cut(s) 256, 397
BmsI GCATC 5 cut(s) 12, 66, 220, 263, 568
BpiI GAAGAC 2 cut(s) 503, 828
BpmI CTGGAG 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 54
Bpu1102I GCTNAGC 1 cut(s) 744
BsaAI YACGTR 2 cut(s) 451, 713
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 2 cut(s) 396, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse1I ACTGG 4 cut(s) 352, 446, 505, 545
BseBI CCWGG 2 cut(s) 256, 397
BseDI CCNNGG 2 cut(s) 396, 505
BseGI GGATG 2 cut(s) 406, 530
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMII CTCAG 2 cut(s) 68, 735
BseNI ACTGG 4 cut(s) 352, 446, 505, 545
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 3 cut(s) 91, 404, 735
BshFI GGCC 5 cut(s) 351, 504, 766, 798, 837
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 2 cut(s) 36, 849
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 5 cut(s) 351, 504, 766, 798, 837
Bso31I GGTCTC 1 cut(s) 849
Bsp1286I GDGCHC 2 cut(s) 296, 640
Bsp1407I TGTACA 1 cut(s) 364
Bsp143I GATC 1 cut(s) 721
Bsp1720I GCTNAGC 1 cut(s) 744
BspANI GGCC 5 cut(s) 351, 504, 766, 798, 837
BspCNI CTCAG 2 cut(s) 67, 736
BspMAI CTGCAG 1 cut(s) 753
BspQI GCTCTTC 1 cut(s) 773
BspTNI GGTCTC 1 cut(s) 849
BsrGI TGTACA 1 cut(s) 364
BsrI ACTGG 4 cut(s) 352, 446, 505, 545
BssECI CCNNGG 2 cut(s) 396, 505
BssMI GATC 1 cut(s) 721
Bst2UI CCWGG 2 cut(s) 256, 397
Bst4CI ACNGT 2 cut(s) 283, 331
Bst6I CTCTTC 2 cut(s) 33, 773
BstAUI TGTACA 1 cut(s) 364
BstBAI YACGTR 2 cut(s) 451, 713
BstDEI CTNAG 4 cut(s) 54, 171, 374, 744
BstDSI CCRYGG 1 cut(s) 505
BstEII GGTNACC 1 cut(s) 568
BstF5I GGATG 2 cut(s) 406, 530
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 1 cut(s) 724
BstMAI GTCTC 2 cut(s) 36, 849
BstMBI GATC 1 cut(s) 721
BstMWI GCNNNNNNNGC 2 cut(s) 260, 428
BstNI CCWGG 2 cut(s) 256, 397
BstPI GGTNACC 1 cut(s) 568
BstSCI CCNGG 2 cut(s) 254, 395
BstSFI CTRYAG 1 cut(s) 749
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 3 cut(s) 91, 404, 735
BstV2I GAAGAC 2 cut(s) 503, 828
BstXI CCANNNNNNTGG 1 cut(s) 403
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 5 cut(s) 351, 504, 766, 798, 837
BtgI CCRYGG 1 cut(s) 505
BtgZI GCGATG 1 cut(s) 641
BtsCI GGATG 2 cut(s) 406, 530
CfoI GCGC 1 cut(s) 583
Cfr13I GGNCC 1 cut(s) 509
Csp6I GTAC 2 cut(s) 99, 365
CviAII CATG 6 cut(s) 28, 241, 368, 700, 758, 784
CviQI GTAC 2 cut(s) 99, 365
DdeI CTNAG 4 cut(s) 54, 171, 374, 744
DpnI GATC 1 cut(s) 723
DpnII GATC 1 cut(s) 721
DraI TTTAAA 1 cut(s) 487
EaeI YGGCCR 4 cut(s) 349, 502, 796, 835
Eam1104I CTCTTC 2 cut(s) 33, 773
EarI CTCTTC 2 cut(s) 33, 773
Eco24I GRGCYC 1 cut(s) 640
Eco31I GGTCTC 1 cut(s) 849
Eco47I GGWCC 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 254, 395
EcoT22I ATGCAT 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 640
FaeI CATG 6 cut(s) 31, 244, 371, 703, 761, 787
FatI CATG 6 cut(s) 27, 240, 367, 699, 757, 783
FbaI TGATCA 1 cut(s) 721
FblI GTMKAC 1 cut(s) 184
Fnu4HI GCNGC 3 cut(s) 80, 393, 749
FokI GGATG 2 cut(s) 413, 537
FriOI GRGCYC 1 cut(s) 640
Fsp4HI GCNGC 3 cut(s) 80, 393, 749
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 582
GluI GCNGC 3 cut(s) 80, 393, 749
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 5 cut(s) 351, 504, 766, 798, 837
HhaI GCGC 1 cut(s) 583
Hin1II CATG 6 cut(s) 31, 244, 371, 703, 761, 787
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 2 cut(s) 734, 856
HphI GGTGA 4 cut(s) 295, 562, 641, 787
Hpy166II GTNNAC 4 cut(s) 185, 318, 512, 716
Hpy188I TCNGA 2 cut(s) 358, 493
Hpy188III TCNNGA 2 cut(s) 35, 853
Hpy8I GTNNAC 4 cut(s) 185, 318, 512, 716
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 2 cut(s) 283, 331
HpyCH4IV ACGT 2 cut(s) 450, 712
HpyCH4V TGCA 9 cut(s) 79, 221, 233, 244, 263, 276, 431, 703, 751
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 428
HpyF3I CTNAG 4 cut(s) 54, 171, 374, 744
HpySE526I ACGT 2 cut(s) 450, 712
Hsp92II CATG 6 cut(s) 31, 244, 371, 703, 761, 787
HspAI GCGC 1 cut(s) 581
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 1 cut(s) 721
LguI GCTCTTC 1 cut(s) 773
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 3 cut(s) 91, 404, 735
LweI GCATC 5 cut(s) 12, 66, 220, 263, 568
MaeI CTAG 1 cut(s) 35
MaeII ACGT 2 cut(s) 450, 712
MaeIII GTNAC 4 cut(s) 67, 446, 461, 568
MalI GATC 1 cut(s) 723
MboI GATC 1 cut(s) 721
MboII GAAGA 5 cut(s) 50, 149, 508, 790, 833
MfeI CAATTG 1 cut(s) 661
MhlI GDGCHC 2 cut(s) 296, 640
MlsI TGGCCA 3 cut(s) 351, 798, 837
MluCI AATT 5 cut(s) 8, 114, 308, 481, 661
MluNI TGGCCA 3 cut(s) 351, 798, 837
MlyI GAGTC 2 cut(s) 728, 850
MnlI CCTC 4 cut(s) 241, 514, 756, 834
Mox20I TGGCCA 3 cut(s) 351, 798, 837
Mph1103I ATGCAT 1 cut(s) 265
MroXI GAANNNNTTC 1 cut(s) 330
MscI TGGCCA 3 cut(s) 351, 798, 837
MseI TTAA 2 cut(s) 486, 871
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 3 cut(s) 351, 798, 837
MspR9I CCNGG 2 cut(s) 256, 397
MunI CAATTG 1 cut(s) 661
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 2 cut(s) 256, 397
MwoI GCNNNNNNNGC 2 cut(s) 260, 428
NdeII GATC 1 cut(s) 721
NlaIII CATG 6 cut(s) 31, 244, 371, 703, 761, 787
NmuCI GTSAC 2 cut(s) 67, 568
NsiI ATGCAT 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 773
PctI GAATGC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 330
PkrI GCNGC 3 cut(s) 81, 394, 750
PleI GAGTC 2 cut(s) 728, 850
PpsI GAGTC 2 cut(s) 728, 850
Ppu21I YACGTR 2 cut(s) 451, 713
Psp6I CCWGG 2 cut(s) 254, 395
PspEI GGTNACC 1 cut(s) 568
PspGI CCWGG 2 cut(s) 254, 395
PspPI GGNCC 1 cut(s) 509
PstI CTGCAG 1 cut(s) 753
RsaI GTAC 2 cut(s) 100, 366
RsaNI GTAC 2 cut(s) 99, 365
RseI CAYNNNNRTG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 773
SaqAI TTAA 2 cut(s) 486, 871
SatI GCNGC 3 cut(s) 80, 393, 749
Sau3AI GATC 1 cut(s) 721
Sau96I GGNCC 1 cut(s) 509
SchI GAGTC 2 cut(s) 728, 850
ScrFI CCNGG 2 cut(s) 256, 397
SduI GDGCHC 2 cut(s) 296, 640
SfaNI GCATC 5 cut(s) 12, 66, 220, 263, 568
SfcI CTRYAG 1 cut(s) 749
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 5 cut(s) 8, 114, 308, 481, 661
SspI AATATT 1 cut(s) 387
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 254, 395
TaaI ACNGT 2 cut(s) 283, 331
TaiI ACGT 2 cut(s) 453, 715
TaqI TCGA 1 cut(s) 854
TasI AATT 5 cut(s) 8, 114, 308, 481, 661
TatI WGTACW 1 cut(s) 364
Tru1I TTAA 2 cut(s) 486, 871
Tru9I TTAA 2 cut(s) 486, 871
TseFI GTSAC 2 cut(s) 67, 568
TseI GCWGC 3 cut(s) 79, 392, 748
Tsp45I GTSAC 2 cut(s) 67, 568
TspDTI ATGAA 5 cut(s) 17, 323, 414, 696, 834
TspGWI ACGGA 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 509
XapI RAATTY 3 cut(s) 8, 114, 308
XbaI TCTAGA 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.