Rh5DG425500

Phosphatidylinositol transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008855
Physical Location & Seq
Forward (+)
67913707 .. 67917737
4031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG425500.1

Sequence Viewer

Length: 894 bp
ATGGTTCTGATGAAGGAGTTTCGTATTGTGATGCCCATGTCTCTAGAAGAGTATCAAGTTGCTCAGGCATACATGGTCACAAAGATGCAGCAACAGAACACGAATAGTACGGAAGGGGTGGAAATTTTAGAGAATAAATCCTTTGAAGATGATGTCTTTGGAAAGGGTCAATACACTTCTAAGGTTTATCGTCTACAAAGCAAAGCTCCTACTTGGCTTACAACTTTTGCACCAGCAGATGCACTCATCATGCAAGAGGAAGCCTGGAACGCATACCCAAGATGCAAAACAGGTTTGCCATGTCTTTATCTTTGCCCATACTTCACCAAATTTCAGTTGACCATTGAAACTGTTCATAGGGCTGATAATGGTCAGTCCGAAAATGTACATGGTCTAAGCAAAGAAATATTAGCAGCCAGGGATGTGGAAATTGTTGATATAGCTTCATCTGCAAGGGATTACTGGAGTTACGTGGTTGGTAGTAACAATACAGACTTCTCTAATTTTAAATCAGAGAAGACTGGCCGTGGTCCACTTTTAGAGGGATGGCAGGATACGTCAAGTCCAGTTATGACAGCCTACAAGTTGGTCACCATAGATGCGCCATATTGGGGATTTGGCTATAAACTTGAACAAGCTTTGCTATTGGGTGAAAGGGCTCTTTTCCTTGAAAGTCACCGCAATTGTTTTGCTTGGATAGATGAATGGTTTGGAATGACTATGCAACAGCTACGTGAACTTGATCAACAAAGCGACTCATTAGCTGAGCTGCAGAAACATGGGAGGCCAACTTTGGTGAAGAGCATGGACGATTTTGGCCAGAGATTATCACCCGAAAGTGAAGACATTTATCTTGGCCAGAGGTCTCAACTCTCGACTCAACTATCAACTTAA

Protein Analysis

297

Amino Acids

33.97

Weight (kDa)

4.95

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IP_trans PF02121 2 - 250 4.1e-91 Phosphatidylinositol transfer protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 193
AciI CCGC 1 cut(s) 679
AcoI YGGCCR 3 cut(s) 523, 817, 856
AcsI RAATTY 2 cut(s) 123, 329
AfaI GTAC 2 cut(s) 109, 387
AfiI CCNNNNNNNGG 1 cut(s) 611
AgsI TTSAA 4 cut(s) 146, 347, 632, 671
AjnI CCWGG 2 cut(s) 263, 416
AluBI AGCT 6 cut(s) 206, 443, 638, 730, 764, 769
AluI AGCT 6 cut(s) 206, 443, 638, 730, 764, 769
Alw26I GTCTC 2 cut(s) 45, 870
AoxI GGCC 4 cut(s) 523, 785, 817, 856
ApeKI GCWGC 3 cut(s) 88, 413, 769
ApoI RAATTY 2 cut(s) 123, 329
Asp700I GAANNNNTTC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 1 cut(s) 530
AsuHPI GGTGA 6 cut(s) 316, 583, 662, 668, 808, 822
AvaII GGWCC 1 cut(s) 530
BalI TGGCCA 2 cut(s) 819, 858
BanII GRGCYC 1 cut(s) 661
BbsI GAAGAC 2 cut(s) 524, 849
BbvI GCAGC 3 cut(s) 100, 425, 756
BccI CCATC 1 cut(s) 540
BceAI ACGGC 1 cut(s) 510
BciT130I CCWGG 2 cut(s) 265, 418
BciVI GTATCC 1 cut(s) 547
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 2 cut(s) 45, 870
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 770
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 3 cut(s) 89, 414, 770
BlpI GCTNAGC 1 cut(s) 765
BlsI GCNGC 3 cut(s) 90, 415, 771
Bme1390I CCNGG 2 cut(s) 265, 418
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 1 cut(s) 530
BmrFI CCNGG 2 cut(s) 265, 418
BmsI GCATC 5 cut(s) 21, 75, 229, 272, 589
BpiI GAAGAC 2 cut(s) 524, 849
BpmI CTGGAG 1 cut(s) 484
Bpu10I CCTNAGC 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 765
BsaAI YACGTR 2 cut(s) 472, 734
BsaI GGTCTC 1 cut(s) 870
BsaJI CCNNGG 2 cut(s) 417, 526
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse1I ACTGG 3 cut(s) 467, 526, 566
BseBI CCWGG 2 cut(s) 265, 418
BseDI CCNNGG 2 cut(s) 417, 526
BseGI GGATG 2 cut(s) 427, 551
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMII CTCAG 2 cut(s) 77, 756
BseNI ACTGG 3 cut(s) 467, 526, 566
BseXI GCAGC 3 cut(s) 100, 425, 756
BshFI GGCC 4 cut(s) 525, 787, 819, 858
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 2 cut(s) 45, 870
BsnI GGCC 4 cut(s) 525, 787, 819, 858
Bso31I GGTCTC 1 cut(s) 870
Bsp1286I GDGCHC 1 cut(s) 661
Bsp1407I TGTACA 1 cut(s) 385
Bsp143I GATC 1 cut(s) 742
Bsp1720I GCTNAGC 1 cut(s) 765
BspACI CCGC 1 cut(s) 679
BspANI GGCC 4 cut(s) 525, 787, 819, 858
BspCNI CTCAG 2 cut(s) 76, 757
BspMAI CTGCAG 1 cut(s) 774
BspQI GCTCTTC 1 cut(s) 794
BspTNI GGTCTC 1 cut(s) 870
BsrGI TGTACA 1 cut(s) 385
BsrI ACTGG 3 cut(s) 467, 526, 566
BssECI CCNNGG 2 cut(s) 417, 526
BssMI GATC 1 cut(s) 742
Bst2UI CCWGG 2 cut(s) 265, 418
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 2 cut(s) 42, 794
BstAUI TGTACA 1 cut(s) 385
BstBAI YACGTR 2 cut(s) 472, 734
BstDEI CTNAG 4 cut(s) 63, 180, 395, 765
BstDSI CCRYGG 1 cut(s) 526
BstEII GGTNACC 1 cut(s) 589
BstF5I GGATG 2 cut(s) 427, 551
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 1 cut(s) 745
BstMAI GTCTC 2 cut(s) 45, 870
BstMBI GATC 1 cut(s) 742
BstMWI GCNNNNNNNGC 2 cut(s) 269, 449
BstNI CCWGG 2 cut(s) 265, 418
BstPI GGTNACC 1 cut(s) 589
BstSCI CCNGG 2 cut(s) 263, 416
BstSFI CTRYAG 1 cut(s) 770
BstV1I GCAGC 3 cut(s) 100, 425, 756
BstV2I GAAGAC 2 cut(s) 524, 849
BstXI CCANNNNNNTGG 1 cut(s) 424
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 4 cut(s) 525, 787, 819, 858
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 2 cut(s) 427, 551
CfoI GCGC 1 cut(s) 604
Cfr13I GGNCC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 108, 386
CviAII CATG 7 cut(s) 37, 73, 250, 300, 389, 779, 805
CviQI GTAC 2 cut(s) 108, 386
DdeI CTNAG 4 cut(s) 63, 180, 395, 765
DpnI GATC 1 cut(s) 744
DpnII GATC 1 cut(s) 742
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 3 cut(s) 523, 817, 856
Eam1104I CTCTTC 2 cut(s) 42, 794
EarI CTCTTC 2 cut(s) 42, 794
Eco24I GRGCYC 1 cut(s) 661
Eco31I GGTCTC 1 cut(s) 870
Eco47I GGWCC 1 cut(s) 530
Eco91I GGTNACC 1 cut(s) 589
EcoO65I GGTNACC 1 cut(s) 589
EcoRII CCWGG 2 cut(s) 263, 416
EcoT38I GRGCYC 1 cut(s) 661
FaeI CATG 7 cut(s) 40, 76, 253, 303, 392, 782, 808
FatI CATG 7 cut(s) 36, 72, 249, 299, 388, 778, 804
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 193
Fnu4HI GCNGC 3 cut(s) 89, 414, 770
FokI GGATG 2 cut(s) 434, 558
FriOI GRGCYC 1 cut(s) 661
Fsp4HI GCNGC 3 cut(s) 89, 414, 770
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 603
GluI GCNGC 3 cut(s) 89, 414, 770
GsuI CTGGAG 1 cut(s) 484
HaeIII GGCC 4 cut(s) 525, 787, 819, 858
HhaI GCGC 1 cut(s) 604
Hin1II CATG 7 cut(s) 40, 76, 253, 303, 392, 782, 808
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 2 cut(s) 755, 877
HphI GGTGA 6 cut(s) 316, 583, 662, 668, 808, 822
Hpy166II GTNNAC 4 cut(s) 194, 339, 533, 737
Hpy188I TCNGA 3 cut(s) 9, 379, 514
Hpy188III TCNNGA 2 cut(s) 44, 874
Hpy8I GTNNAC 4 cut(s) 194, 339, 533, 737
HpyAV CCTTC 2 cut(s) 7, 107
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 3 cut(s) 471, 557, 733
HpyCH4V TGCA 8 cut(s) 88, 230, 242, 253, 285, 452, 724, 772
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 449
HpyF3I CTNAG 4 cut(s) 63, 180, 395, 765
HpySE526I ACGT 3 cut(s) 471, 557, 733
Hsp92II CATG 7 cut(s) 40, 76, 253, 303, 392, 782, 808
HspAI GCGC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 1 cut(s) 742
LguI GCTCTTC 1 cut(s) 794
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 3 cut(s) 100, 425, 756
LweI GCATC 5 cut(s) 21, 75, 229, 272, 589
MaeI CTAG 1 cut(s) 44
MaeII ACGT 3 cut(s) 471, 557, 733
MaeIII GTNAC 5 cut(s) 76, 467, 482, 589, 674
MalI GATC 1 cut(s) 744
MboI GATC 1 cut(s) 742
MboII GAAGA 5 cut(s) 59, 158, 529, 811, 854
MfeI CAATTG 1 cut(s) 682
MhlI GDGCHC 1 cut(s) 661
MlsI TGGCCA 2 cut(s) 819, 858
MluCI AATT 5 cut(s) 123, 329, 429, 502, 682
MluNI TGGCCA 2 cut(s) 819, 858
MlyI GAGTC 2 cut(s) 749, 871
MnlI CCTC 4 cut(s) 250, 535, 777, 855
Mox20I TGGCCA 2 cut(s) 819, 858
MroXI GAANNNNTTC 1 cut(s) 351
MscI TGGCCA 2 cut(s) 819, 858
MseI TTAA 2 cut(s) 507, 892
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 2 cut(s) 819, 858
MspR9I CCNGG 2 cut(s) 265, 418
MunI CAATTG 1 cut(s) 682
MvaI CCWGG 2 cut(s) 265, 418
MwoI GCNNNNNNNGC 2 cut(s) 269, 449
NdeII GATC 1 cut(s) 742
NlaIII CATG 7 cut(s) 40, 76, 253, 303, 392, 782, 808
NmuCI GTSAC 3 cut(s) 76, 589, 674
PciSI GCTCTTC 1 cut(s) 794
PdmI GAANNNNTTC 1 cut(s) 351
PkrI GCNGC 3 cut(s) 90, 415, 771
PleI GAGTC 2 cut(s) 749, 871
PpsI GAGTC 2 cut(s) 749, 871
Ppu21I YACGTR 2 cut(s) 472, 734
Psp6I CCWGG 2 cut(s) 263, 416
PspEI GGTNACC 1 cut(s) 589
PspGI CCWGG 2 cut(s) 263, 416
PspPI GGNCC 1 cut(s) 530
PstI CTGCAG 1 cut(s) 774
RsaI GTAC 2 cut(s) 109, 387
RsaNI GTAC 2 cut(s) 108, 386
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 794
SaqAI TTAA 2 cut(s) 507, 892
SatI GCNGC 3 cut(s) 89, 414, 770
Sau3AI GATC 1 cut(s) 742
Sau96I GGNCC 1 cut(s) 530
SchI GAGTC 2 cut(s) 749, 871
ScrFI CCNGG 2 cut(s) 265, 418
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 5 cut(s) 21, 75, 229, 272, 589
SfcI CTRYAG 1 cut(s) 770
SinI GGWCC 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 5 cut(s) 123, 329, 429, 502, 682
SsiI CCGC 1 cut(s) 679
SspI AATATT 1 cut(s) 408
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 2 cut(s) 263, 416
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 3 cut(s) 474, 560, 736
TaqI TCGA 1 cut(s) 875
TasI AATT 5 cut(s) 123, 329, 429, 502, 682
TatI WGTACW 1 cut(s) 385
Tru1I TTAA 2 cut(s) 507, 892
Tru9I TTAA 2 cut(s) 507, 892
TseFI GTSAC 3 cut(s) 76, 589, 674
TseI GCWGC 3 cut(s) 88, 413, 769
Tsp45I GTSAC 3 cut(s) 76, 589, 674
TspDTI ATGAA 4 cut(s) 26, 344, 435, 717
TspGWI ACGGA 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 530
XapI RAATTY 2 cut(s) 123, 329
XbaI TCTAGA 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.