Rw5G037540

Phosphatidylinositol transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008855
Physical Location & Seq
Forward (+)
66151168 .. 66155907
4740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037540.1

Sequence Viewer

Length: 882 bp
ATGGTTCTGATGAAGGAGTTTCGTATTGTGATGCCCATGTCTCTAGAAGAGTATCAAGTTGCTCAGGCATATATGGTCACAAAGATGCAGCAACAGAACACGAATAGTACGGAAGGGGTGGAAATTTTAGAGAATAAATCCTTTGAAGACGATGTCTTTGGAAAGGGTCAATACACTTCTAAGGTTTATCGTCTACAAAGCAAAGCTCCTACTTGGCTTACAACTTTTGCACCAGCAGATGCACTCATCATGCAAGAGGAAGCCTGGAACGCATACCCAAGATGCAAAACAGTAATCAAGTGCCCATACTTCACCAAATTTCAGTTGACCATTGAAACTGTTCATAGGGCTGATAATGGTCAGTCCGAAAATGTACATGGTCTAAGCAAAGAAATATTAGCAGCCAGGGATGTGGAAATTGTTGATATAGCTTCATCTGCAAGGGATTACTGGAGTTACGTGGTTGGTAGTAACAATACAGACTTCTCTAATTTTAAATCAGAGAAGACTGGCCGTGGTCCACTTTTAGAGGGATGGCAGGATACGTCAAGTCCAGTTATGACAGCCTACAAGTTGGTCACCATAGATGCGCCATATTGGGGATTTGGCTATAAACTTGAACAAGCTTTGCTATTGGGTGAAAGGGCTCTTTTCCTTGAAAGTCACCGCAATTGTTTTGCTTGGATAGATGAATGGTTTGGAATGACTATGCAACAGCTACGTGAACTTGATCAACAAAGCGACTCATTAGCTGAGCTGCAGAAACATGGGAGGCCAACATTGGTGAAGAGCATGGACGATTTTGGCCAGAGATTATCACCCGAAAGTGAAGACATTTATCTTGGCCAGAGGTCTCAACTCTCGACTCAACTATCAACTTAA

Protein Analysis

293

Amino Acids

33.55

Weight (kDa)

5.02

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IP_trans PF02121 2 - 246 2.6e-94 Phosphatidylinositol transfer protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 193
AciI CCGC 1 cut(s) 667
AcoI YGGCCR 3 cut(s) 511, 805, 844
AcsI RAATTY 2 cut(s) 123, 317
AfaI GTAC 2 cut(s) 109, 375
AfiI CCNNNNNNNGG 1 cut(s) 599
AgsI TTSAA 4 cut(s) 146, 335, 620, 659
AjnI CCWGG 2 cut(s) 263, 404
AluBI AGCT 6 cut(s) 206, 431, 626, 718, 752, 757
AluI AGCT 6 cut(s) 206, 431, 626, 718, 752, 757
Alw26I GTCTC 2 cut(s) 45, 858
AoxI GGCC 4 cut(s) 511, 773, 805, 844
ApeKI GCWGC 3 cut(s) 88, 401, 757
ApoI RAATTY 2 cut(s) 123, 317
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
Asp700I GAANNNNTTC 1 cut(s) 339
AspLEI GCGC 1 cut(s) 592
AspS9I GGNCC 1 cut(s) 518
AsuHPI GGTGA 6 cut(s) 304, 571, 650, 656, 796, 810
AvaII GGWCC 1 cut(s) 518
BaeGI GKGCMC 1 cut(s) 305
BalI TGGCCA 2 cut(s) 807, 846
BanII GRGCYC 1 cut(s) 649
BbsI GAAGAC 3 cut(s) 153, 512, 837
BbvI GCAGC 3 cut(s) 100, 413, 744
BccI CCATC 1 cut(s) 528
BceAI ACGGC 1 cut(s) 498
BciT130I CCWGG 2 cut(s) 265, 406
BciVI GTATCC 1 cut(s) 535
BclI TGATCA 1 cut(s) 730
BcoDI GTCTC 2 cut(s) 45, 858
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 758
BfuI GTATCC 1 cut(s) 535
BisI GCNGC 3 cut(s) 89, 402, 758
BlpI GCTNAGC 1 cut(s) 753
BlsI GCNGC 3 cut(s) 90, 403, 759
Bme1390I CCNGG 2 cut(s) 265, 406
Bme18I GGWCC 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 518
BmrFI CCNGG 2 cut(s) 265, 406
BmsI GCATC 5 cut(s) 21, 75, 229, 272, 577
BpiI GAAGAC 3 cut(s) 153, 512, 837
BpmI CTGGAG 1 cut(s) 472
Bpu10I CCTNAGC 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 753
BsaAI YACGTR 2 cut(s) 460, 722
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 2 cut(s) 405, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse1I ACTGG 3 cut(s) 455, 514, 554
BseBI CCWGG 2 cut(s) 265, 406
BseDI CCNNGG 2 cut(s) 405, 514
BseGI GGATG 2 cut(s) 415, 539
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMII CTCAG 2 cut(s) 77, 744
BseNI ACTGG 3 cut(s) 455, 514, 554
BseSI GKGCMC 1 cut(s) 305
BseXI GCAGC 3 cut(s) 100, 413, 744
BshFI GGCC 4 cut(s) 513, 775, 807, 846
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 2 cut(s) 45, 858
BsnI GGCC 4 cut(s) 513, 775, 807, 846
Bso31I GGTCTC 1 cut(s) 858
Bsp1286I GDGCHC 2 cut(s) 305, 649
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 1 cut(s) 730
Bsp1720I GCTNAGC 1 cut(s) 753
BspACI CCGC 1 cut(s) 667
BspANI GGCC 4 cut(s) 513, 775, 807, 846
BspCNI CTCAG 2 cut(s) 76, 745
BspMAI CTGCAG 1 cut(s) 762
BspQI GCTCTTC 1 cut(s) 782
BspTNI GGTCTC 1 cut(s) 858
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 3 cut(s) 455, 514, 554
BssECI CCNNGG 2 cut(s) 405, 514
BssMI GATC 1 cut(s) 730
Bst2UI CCWGG 2 cut(s) 265, 406
Bst4CI ACNGT 2 cut(s) 292, 340
Bst6I CTCTTC 2 cut(s) 42, 782
BstAUI TGTACA 1 cut(s) 373
BstBAI YACGTR 2 cut(s) 460, 722
BstDEI CTNAG 4 cut(s) 63, 180, 383, 753
BstDSI CCRYGG 1 cut(s) 514
BstEII GGTNACC 1 cut(s) 577
BstF5I GGATG 2 cut(s) 415, 539
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 1 cut(s) 733
BstMAI GTCTC 2 cut(s) 45, 858
BstMBI GATC 1 cut(s) 730
BstMWI GCNNNNNNNGC 2 cut(s) 269, 437
BstNI CCWGG 2 cut(s) 265, 406
BstPI GGTNACC 1 cut(s) 577
BstSCI CCNGG 2 cut(s) 263, 404
BstSFI CTRYAG 1 cut(s) 758
BstSLI GKGCMC 1 cut(s) 305
BstV1I GCAGC 3 cut(s) 100, 413, 744
BstV2I GAAGAC 3 cut(s) 153, 512, 837
BstXI CCANNNNNNTGG 1 cut(s) 412
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 4 cut(s) 513, 775, 807, 846
BtgI CCRYGG 1 cut(s) 514
BtsCI GGATG 2 cut(s) 415, 539
CfoI GCGC 1 cut(s) 592
Cfr13I GGNCC 1 cut(s) 518
Csp6I GTAC 2 cut(s) 108, 374
CviAII CATG 5 cut(s) 37, 250, 377, 767, 793
CviQI GTAC 2 cut(s) 108, 374
DdeI CTNAG 4 cut(s) 63, 180, 383, 753
DpnI GATC 1 cut(s) 732
DpnII GATC 1 cut(s) 730
DraI TTTAAA 1 cut(s) 496
EaeI YGGCCR 3 cut(s) 511, 805, 844
Eam1104I CTCTTC 2 cut(s) 42, 782
EarI CTCTTC 2 cut(s) 42, 782
Eco24I GRGCYC 1 cut(s) 649
Eco31I GGTCTC 1 cut(s) 858
Eco47I GGWCC 1 cut(s) 518
Eco91I GGTNACC 1 cut(s) 577
EcoO65I GGTNACC 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 263, 404
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 5 cut(s) 40, 253, 380, 770, 796
FatI CATG 5 cut(s) 36, 249, 376, 766, 792
FbaI TGATCA 1 cut(s) 730
FblI GTMKAC 1 cut(s) 193
Fnu4HI GCNGC 3 cut(s) 89, 402, 758
FokI GGATG 2 cut(s) 422, 546
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 3 cut(s) 89, 402, 758
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 591
GluI GCNGC 3 cut(s) 89, 402, 758
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 4 cut(s) 513, 775, 807, 846
HhaI GCGC 1 cut(s) 592
Hin1II CATG 5 cut(s) 40, 253, 380, 770, 796
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 2 cut(s) 743, 865
HphI GGTGA 6 cut(s) 304, 571, 650, 656, 796, 810
Hpy166II GTNNAC 4 cut(s) 194, 327, 521, 725
Hpy188I TCNGA 3 cut(s) 9, 367, 502
Hpy188III TCNNGA 2 cut(s) 44, 862
Hpy8I GTNNAC 4 cut(s) 194, 327, 521, 725
HpyAV CCTTC 2 cut(s) 7, 107
HpyCH4III ACNGT 2 cut(s) 292, 340
HpyCH4IV ACGT 3 cut(s) 459, 545, 721
HpyCH4V TGCA 8 cut(s) 88, 230, 242, 253, 285, 440, 712, 760
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 437
HpyF3I CTNAG 4 cut(s) 63, 180, 383, 753
HpySE526I ACGT 3 cut(s) 459, 545, 721
Hsp92II CATG 5 cut(s) 40, 253, 380, 770, 796
HspAI GCGC 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 1 cut(s) 730
LguI GCTCTTC 1 cut(s) 782
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 3 cut(s) 100, 413, 744
LweI GCATC 5 cut(s) 21, 75, 229, 272, 577
MaeI CTAG 1 cut(s) 44
MaeII ACGT 3 cut(s) 459, 545, 721
MaeIII GTNAC 5 cut(s) 76, 455, 470, 577, 662
MalI GATC 1 cut(s) 732
MboI GATC 1 cut(s) 730
MboII GAAGA 5 cut(s) 59, 158, 517, 799, 842
MfeI CAATTG 1 cut(s) 670
MhlI GDGCHC 2 cut(s) 305, 649
MlsI TGGCCA 2 cut(s) 807, 846
MluCI AATT 5 cut(s) 123, 317, 417, 490, 670
MluNI TGGCCA 2 cut(s) 807, 846
MlyI GAGTC 2 cut(s) 737, 859
MnlI CCTC 4 cut(s) 250, 523, 765, 843
Mox20I TGGCCA 2 cut(s) 807, 846
MroXI GAANNNNTTC 1 cut(s) 339
MscI TGGCCA 2 cut(s) 807, 846
MseI TTAA 2 cut(s) 495, 880
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 2 cut(s) 807, 846
MspR9I CCNGG 2 cut(s) 265, 406
MunI CAATTG 1 cut(s) 670
MvaI CCWGG 2 cut(s) 265, 406
MwoI GCNNNNNNNGC 2 cut(s) 269, 437
NdeII GATC 1 cut(s) 730
NlaIII CATG 5 cut(s) 40, 253, 380, 770, 796
NmuCI GTSAC 3 cut(s) 76, 577, 662
PciSI GCTCTTC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 339
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 90, 403, 759
PleI GAGTC 2 cut(s) 737, 859
PpsI GAGTC 2 cut(s) 737, 859
Ppu21I YACGTR 2 cut(s) 460, 722
Psp6I CCWGG 2 cut(s) 263, 404
PspEI GGTNACC 1 cut(s) 577
PspGI CCWGG 2 cut(s) 263, 404
PspPI GGNCC 1 cut(s) 518
PstI CTGCAG 1 cut(s) 762
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 2 cut(s) 109, 375
RsaNI GTAC 2 cut(s) 108, 374
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 782
SaqAI TTAA 2 cut(s) 495, 880
SatI GCNGC 3 cut(s) 89, 402, 758
Sau3AI GATC 1 cut(s) 730
Sau96I GGNCC 1 cut(s) 518
SchI GAGTC 2 cut(s) 737, 859
ScrFI CCNGG 2 cut(s) 265, 406
SduI GDGCHC 2 cut(s) 305, 649
SfaNI GCATC 5 cut(s) 21, 75, 229, 272, 577
SfcI CTRYAG 1 cut(s) 758
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 5 cut(s) 123, 317, 417, 490, 670
SsiI CCGC 1 cut(s) 667
SspI AATATT 1 cut(s) 396
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 2 cut(s) 263, 404
TaaI ACNGT 2 cut(s) 292, 340
TaiI ACGT 3 cut(s) 462, 548, 724
TaqI TCGA 1 cut(s) 863
TasI AATT 5 cut(s) 123, 317, 417, 490, 670
TatI WGTACW 1 cut(s) 373
Tru1I TTAA 2 cut(s) 495, 880
Tru9I TTAA 2 cut(s) 495, 880
TseFI GTSAC 3 cut(s) 76, 577, 662
TseI GCWGC 3 cut(s) 88, 401, 757
Tsp45I GTSAC 3 cut(s) 76, 577, 662
TspDTI ATGAA 4 cut(s) 26, 332, 423, 705
TspGWI ACGGA 1 cut(s) 125
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 2 cut(s) 123, 317
XbaI TCTAGA 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.