MD03G1111000.v1.1

Phosphatidylinositol transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008855
Physical Location & Seq
Reverse (-)
9687269 .. 9691813
4545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1111000.v1.1.491

Sequence Viewer

Length: 864 bp
ATGGTTCTGATGAAGGAATTTCGGATTGTCATGCCCATGTCGTTGGAAGAGTATCGAGTAGCCCAGACATATATGGTCATGAAGATGCAGGAACAGAACACAAACAGTACCGAAGGGGTGGAAATAGTAGAGAGTAAATCCTTTGAAGATGATTTATTTGGAAAGGGTCACTACAGTTCTAAAATTTATCGTCTACAAAGCAAAGCTCCTGGTTGGCTTGCCACTTTTGCGCCAGCAGATGCTCTCATCATGCAAGAGGAAGCCTGGAATGCACACCCAAGATGCAAGACAGTAATCAAGTGCCCATACTTCACCAAGTTTCAGCTGACCGTCGAAACAGTTAACAGAGCTGACAATGGCCATTCGGATAATGTTCATGGTCTAAGCAAAGCACAACTAGCAACCAGGGATGTGGAAACTGTTGATATTGCTTCATCTGCAAGGGATTACTGGAGTCACATAGTTGGCACTAACAACACCGACTTTTCCCAATTTACATCAACGAAAACTGGACGTGGTCCACTTTTAGAGGGCTGGCAGGACACGTCGGATCCAGTTATGACAGCCTACAAACTGGTCACCATTGATGCACCATATTGGGGTTTCGGCAATAAACTTGAACGAGCTTTGCTATTGGGTGAAAGGGCTCTTTTCCTAGAAAGCCATCGGAATTGCTTTTCTTGGATTGATGAATGGTCTGGAATGACAGTTGAACAGATACGTGAACGTGAACGACATAGAAACTCAGAGATCCAGGAACTTGGAAGGCCAACTTTGGTGAAGAGCCTAGAAGATATCGACCAGAGATTATTACCTGAAAGCGAAGAAATTTATAGCGGGCAGAGACCTCAACTTTCAACTTAA

Protein Analysis

288

Amino Acids

32.92

Weight (kDa)

5.38

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IP_trans PF02121 2 - 246 6.4e-87 Phosphatidylinositol transfer protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 193
AciI CCGC 1 cut(s) 837
AclWI GGATC 3 cut(s) 545, 558, 745
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 3 cut(s) 17, 183, 828
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 4 cut(s) 146, 620, 713, 858
AjiI CACGTC 2 cut(s) 515, 546
AjnI CCWGG 4 cut(s) 208, 263, 404, 753
AluBI AGCT 4 cut(s) 206, 325, 350, 626
AluI AGCT 4 cut(s) 206, 325, 350, 626
Alw26I GTCTC 1 cut(s) 838
AlwI GGATC 3 cut(s) 545, 558, 745
AoxI GGCC 2 cut(s) 358, 767
ApoI RAATTY 3 cut(s) 17, 183, 828
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 518
AsuHPI GGTGA 4 cut(s) 304, 571, 650, 790
AvaII GGWCC 1 cut(s) 518
BaeGI GKGCMC 1 cut(s) 305
BalI TGGCCA 1 cut(s) 360
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 649
BccI CCATC 1 cut(s) 672
BciT130I CCWGG 4 cut(s) 210, 265, 406, 755
BcoDI GTCTC 1 cut(s) 838
BfaI CTAG 3 cut(s) 398, 656, 788
BfmI CTRYAG 1 cut(s) 172
Bme1390I CCNGG 4 cut(s) 210, 265, 406, 755
Bme18I GGWCC 1 cut(s) 518
BmgBI CACGTC 2 cut(s) 515, 546
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 4 cut(s) 210, 265, 406, 755
BmsI GCATC 4 cut(s) 75, 229, 272, 577
BpmI CTGGAG 1 cut(s) 472
BsaAI YACGTR 1 cut(s) 722
BsaI GGTCTC 1 cut(s) 838
BsaJI CCNNGG 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse1I ACTGG 4 cut(s) 455, 514, 554, 579
BseBI CCWGG 4 cut(s) 210, 265, 406, 755
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 4 cut(s) 455, 514, 554, 579
BseSI GKGCMC 1 cut(s) 305
BshFI GGCC 2 cut(s) 360, 769
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 1 cut(s) 838
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 2 cut(s) 360, 769
Bso31I GGTCTC 1 cut(s) 838
Bsp1286I GDGCHC 2 cut(s) 305, 649
Bsp143I GATC 2 cut(s) 550, 750
BspACI CCGC 1 cut(s) 837
BspANI GGCC 2 cut(s) 360, 769
BspCNI CTCAG 1 cut(s) 758
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 552
BspPI GGATC 3 cut(s) 545, 558, 745
BspQI GCTCTTC 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 838
BsrI ACTGG 4 cut(s) 455, 514, 554, 579
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 2 cut(s) 550, 750
Bst2UI CCWGG 4 cut(s) 210, 265, 406, 755
Bst4CI ACNGT 7 cut(s) 107, 176, 292, 331, 340, 421, 709
Bst6I CTCTTC 2 cut(s) 42, 776
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 4 cut(s) 219, 234, 536, 839
BstDEI CTNAG 2 cut(s) 383, 745
BstEII GGTNACC 1 cut(s) 577
BstF5I GGATG 1 cut(s) 415
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 2 cut(s) 553, 753
BstMAI GTCTC 1 cut(s) 838
BstMBI GATC 2 cut(s) 550, 750
BstMWI GCNNNNNNNGC 4 cut(s) 227, 269, 398, 437
BstNI CCWGG 4 cut(s) 210, 265, 406, 755
BstPI GGTNACC 1 cut(s) 577
BstSCI CCNGG 4 cut(s) 208, 263, 404, 753
BstSFI CTRYAG 1 cut(s) 172
BstSLI GKGCMC 1 cut(s) 305
BstX2I RGATCY 2 cut(s) 550, 750
BstXI CCANNNNNNTGG 3 cut(s) 43, 412, 761
BstYI RGATCY 2 cut(s) 550, 750
BsuRI GGCC 2 cut(s) 360, 769
BtrI CACGTC 2 cut(s) 515, 546
BtsCI GGATG 1 cut(s) 415
Cac8I GCNNGC 4 cut(s) 219, 234, 536, 839
CciI TCATGA 1 cut(s) 78
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 108
CviAII CATG 5 cut(s) 31, 37, 79, 250, 377
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 2 cut(s) 383, 745
DpnI GATC 2 cut(s) 552, 752
DpnII GATC 2 cut(s) 550, 750
EaeI YGGCCR 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 42, 776
EarI CTCTTC 2 cut(s) 42, 776
Eco24I GRGCYC 1 cut(s) 649
Eco31I GGTCTC 1 cut(s) 838
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 518
Eco91I GGTNACC 1 cut(s) 577
EcoO65I GGTNACC 1 cut(s) 577
EcoRII CCWGG 4 cut(s) 208, 263, 404, 753
EcoRV GATATC 1 cut(s) 796
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 5 cut(s) 34, 40, 82, 253, 380
FalI AAGNNNNNCTT 2 cut(s) 757, 789
FatI CATG 5 cut(s) 30, 36, 78, 249, 376
FauI CCCGC 1 cut(s) 830
FblI GTMKAC 1 cut(s) 193
FokI GGATG 1 cut(s) 422
FriOI GRGCYC 1 cut(s) 649
FspBI CTAG 3 cut(s) 398, 656, 788
GlaI GCGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 2 cut(s) 360, 769
HhaI GCGC 1 cut(s) 232
Hin1II CATG 5 cut(s) 34, 40, 82, 253, 380
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 1 cut(s) 454
HpaI GTTAAC 1 cut(s) 343
HphI GGTGA 4 cut(s) 304, 571, 650, 790
Hpy166II GTNNAC 5 cut(s) 194, 343, 521, 725, 731
Hpy188I TCNGA 6 cut(s) 9, 24, 367, 550, 669, 748
Hpy188III TCNNGA 2 cut(s) 79, 699
Hpy8I GTNNAC 5 cut(s) 194, 343, 521, 725, 731
Hpy99I CGWCG 2 cut(s) 335, 550
HpyAV CCTTC 3 cut(s) 7, 107, 759
HpyCH4III ACNGT 7 cut(s) 107, 176, 292, 331, 340, 421, 709
HpyCH4IV ACGT 4 cut(s) 514, 545, 721, 727
HpyCH4V TGCA 6 cut(s) 88, 253, 272, 285, 440, 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 269, 398, 437
HpyF3I CTNAG 2 cut(s) 383, 745
HpySE526I ACGT 4 cut(s) 514, 545, 721, 727
Hsp92II CATG 5 cut(s) 34, 40, 82, 253, 380
HspAI GCGC 1 cut(s) 230
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 2 cut(s) 550, 750
LguI GCTCTTC 1 cut(s) 776
LmnI GCTCC 1 cut(s) 211
LweI GCATC 4 cut(s) 75, 229, 272, 577
MaeI CTAG 3 cut(s) 398, 656, 788
MaeII ACGT 4 cut(s) 514, 545, 721, 727
MaeIII GTNAC 3 cut(s) 167, 455, 577
MalI GATC 2 cut(s) 552, 752
MboI GATC 2 cut(s) 550, 750
MboII GAAGA 6 cut(s) 59, 94, 158, 793, 803, 836
MflI RGATCY 2 cut(s) 550, 750
MhlI GDGCHC 2 cut(s) 305, 649
MlsI TGGCCA 1 cut(s) 360
MluCI AATT 5 cut(s) 17, 183, 491, 670, 828
MluNI TGGCCA 1 cut(s) 360
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 2 cut(s) 24, 528
MnlI CCTC 3 cut(s) 250, 523, 858
Mox20I TGGCCA 1 cut(s) 360
MscI TGGCCA 1 cut(s) 360
MseI TTAA 2 cut(s) 342, 862
MslI CAYNNNNRTG 2 cut(s) 35, 83
Msp20I TGGCCA 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 325
MspR9I CCNGG 4 cut(s) 210, 265, 406, 755
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 4 cut(s) 210, 265, 406, 755
MwoI GCNNNNNNNGC 4 cut(s) 227, 269, 398, 437
NdeII GATC 2 cut(s) 550, 750
NlaIII CATG 5 cut(s) 34, 40, 82, 253, 380
NlaIV GGNNCC 1 cut(s) 552
NmuCI GTSAC 3 cut(s) 167, 455, 577
PagI TCATGA 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 776
PctI GAATGC 1 cut(s) 274
PflFI GACNNNGTC 1 cut(s) 516
PfoI TCCNGGA 1 cut(s) 753
PleI GAGTC 1 cut(s) 462
PpsI GAGTC 1 cut(s) 462
Ppu21I YACGTR 1 cut(s) 722
Psp6I CCWGG 4 cut(s) 208, 263, 404, 753
PspEI GGTNACC 1 cut(s) 577
PspGI CCWGG 4 cut(s) 208, 263, 404, 753
PspN4I GGNNCC 1 cut(s) 552
PspPI GGNCC 1 cut(s) 518
PsuI RGATCY 2 cut(s) 550, 750
PsyI GACNNNGTC 1 cut(s) 516
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
RseI CAYNNNNRTG 2 cut(s) 35, 83
SapI GCTCTTC 1 cut(s) 776
SaqAI TTAA 2 cut(s) 342, 862
Sau3AI GATC 2 cut(s) 550, 750
Sau96I GGNCC 1 cut(s) 518
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 4 cut(s) 210, 265, 406, 755
SduI GDGCHC 2 cut(s) 305, 649
SfaNI GCATC 4 cut(s) 75, 229, 272, 577
SfcI CTRYAG 1 cut(s) 172
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 2 cut(s) 35, 83
Sse9I AATT 5 cut(s) 17, 183, 491, 670, 828
SsiI CCGC 1 cut(s) 837
SspMI CTAG 3 cut(s) 398, 656, 788
StyD4I CCNGG 4 cut(s) 208, 263, 404, 753
TaaI ACNGT 7 cut(s) 107, 176, 292, 331, 340, 421, 709
TaiI ACGT 4 cut(s) 517, 548, 724, 730
TaqI TCGA 3 cut(s) 55, 333, 798
TasI AATT 5 cut(s) 17, 183, 491, 670, 828
Tru1I TTAA 2 cut(s) 342, 862
Tru9I TTAA 2 cut(s) 342, 862
TseFI GTSAC 3 cut(s) 167, 455, 577
Tsp45I GTSAC 3 cut(s) 167, 455, 577
TspDTI ATGAA 5 cut(s) 26, 95, 365, 423, 705
Tth111I GACNNNGTC 1 cut(s) 516
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 3 cut(s) 17, 183, 828
XmiI GTMKAC 1 cut(s) 193
XspI CTAG 3 cut(s) 398, 656, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.