FvH4_3g34270

PAS fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004648
Physical Location & Seq
Forward (+)
29757731 .. 29768550
10820 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34270.t3

Sequence Viewer

Length: 3087 bp
ATGGAAGACGAACCGTCGGAAACAACCCCAGCCTCACTCATTCCTCCACTCCCAAGAGACTCCCGCGGCTCCCTCGAAATCTTCAACCCCTCCTCGACCCGACCCGCCAGCCCGCCTCCCTTTCGCCAATCCAAACCCGCCACCTGGAAAACCTGGCTCGACCCGCGTGACACCCTAAACCCCAAACCCGACCCGTCACCACCACCCATCCCCGCCTCCAAGTCCGGCCGCGACGACGGCACCATCACCTCATGGATGGCCCTCAAGGACTTCCCTCCAACTCCGCCGCCGCCTTCCAAGCCGTCAAAACAGTCCCAGCAGTCCCCGCCCTATATCCAGCAGCAGCAGACCATCTCCGCCGCGGTCAACGACAAGTCAACCCCGGAGCAAGGCGACGCTGCGCAGAGGGCCGCCGAGTGGGGGCTGGTGCTGAAGACGGACACCGAGACCGGGAAGCCGCAGGGAGTGACGACCAGAAACTCCGGCGGACCCGAGGACCCGAGTCACAAACCGGGTACGTCGAGAAGAAACTCCAACAACTCTGTCCGGAGCTCCGGGGAGCTCTCCGACGACGGCGGCGGTGGCGGAGGAATCGGAAAAGGGTTCCCGAGAGTTTCGGAGGACTTGAAAAACGTGCTGTCGACGTTTCAGCAGACGTTTGTGGTGTCGGATGCAACCAAACCCGATTACCCGATCATGTACGCCAGCGCCGGGTTTTTCAAGATGACCGGGTACACCTCCAAGGAGGTCATCGGAAGAAACTGCCGGTTTTTGCAGGGAGCGGATACGGACCCGGAAGACGTGGCGCAGCTGAGGGAGGCGTTGGCGAAGAACACGAGCTATTGTGGGCGGTTGTTGAATTATAAGAAAGATGGGACCCCTTTTTGGAATTTACTTACTATTGCTCCCATCAAGGACGAAACTGGAAAGGTCCTCAAATTCATCGGAATGCAAGTGGAGGTGAGCAAGCACACTGAGGGGTCCAAGGATAAGATGCTGCGCCCCAATGGACTGCCTGAATCTCTCATTCGATATGATGCTCGTCAGAAAGAGAAGGCTACACATTCAGTAACTGAGCTTGTGCAGGCAGTGAGGAGACCGAGGTCACTTAGTGAATCGACAAATCGTCCCTTTAGAAATTCAGGTGGTGGTGGAAGGGGAGACGAGGTAATAGAAGCTCACGCGAGGCGAAGCTCAGAGAGTTTGCCTCGGAGAAACTCGGAGTCCGTGGCACCACCTAGACGTAACTCACTTGGTGATGCTAACTTCTCAATGCATAGCATCAAGGAGGTTCCTGAAAAGAAACAGAAAAAACCTAGACGCCGCTCTTTCATGGGGATTATGAAGAAAAGTCAAACTCAAAGCCAACTCGATGATGATACATTTGATGAGTTTGGAGCTTCTGAGGACGTCCGTGACGACAGTGACAATGATGAGAGACCCATGAGTCTTGACGACAAAGTGAGGAAGAAGGAAATGAGGAAGGGTATGGATCTTGCTACAACGCTGGAACGTATAGAGAAAAACTTTGTCATTACTGATCCAAGGCTGCCTGATAATCCCATTATATTTGCATCCGACAGCTTCTTAGAGCTAACGGAGTACAGCCGCGAAGAAATATTGGGAAGAAATTGCAGATTCCTTCAAGGACCAGAGACTGATCCAGCAACTGTGAAGAAAATAAGAGAGGCAATTGACAACCAGACTGAAGTGACCGTACAGCTCATCAATTACACGAAAAGCGGCAAAAAGTTCTGGAATGTCTTTCATCTGCAGCCTATGCGTGATCATAAGGGAGAAGTACAGTATTTTATTGGAGTACAACTTGATGGCAGTCAGCACATTGAGCCTCTCCAGAACTCTATTCCTGAAGTTGCAGCAAAAGAGAGTGAAAAACTGGTCAAAGAGACTGCAGTGAATGTTGATGAGGCAGCAAGAGAACTTCCGGATGCCAATATGAAACCAGAAGATTTATGGATGAATCATTCGAAGGTCGTTCATCCAAAGCCTCATAGGAAGGATAGCCCCCCTTGGATAGCTATTCAAAAGATTCTAGACAGTGGAGAACAGATAGGATTGAAACATTTTAAGCCTATAAAGCCCTTGGGATCTGGTGATACTGGCAGTGTGCATTTGGTGCAATTGTGTGGAACTGATCAGTACTTCGCCATGAAGGCTATGGATAAGAATATCATGCTCAATCGGAACAAGGTGCATAGAGCTTGTGCGGAGAGAGAGATCCTCGATATGTTGGATCACCCTTTTCTTCCTGCATTATATGCTTCATTTCAGACCAAAACGCATGTTTGCCTGATCACTGATTACTACCCTGGTGGAGAACTCTTCCTACTTTTGGACAGTCAACCAACGAAGGTCCTGAAGGAAGATTCAGTGAGATTTTATGTTGCAGAGGTAGTTGTTGCCTTGGAGTACCTTCATTGTCAAGGGATAATTTACAGGGATTTGAAGCCTGAAAATGTTTTACTTCAGAGCAATGGGCATGTAACCCTGACCGATTTCGATTTATCATGTTTGACATCTTGCAAACCACAGCTTTTACTTCCAACCATTAACGAAAAGAAAAGGCATCACAAACGTCAACACGATCCAATCTTCATGGCTGAACCAATGAGAGCATCAAATTCATTCGTTGGCACGGAAGAGTATATCGCTCCAGAAATCATAACTGGAGCTGGCCATTCTAGTGCAGTAGACTGGTGGGCTCTTGGTATTCTTATATATGAAATGCTCTATGGATATACACCGTTTAGGGGAAAGACAAGGCAAAAGACATTCGCCAATATTCTTCACAAGGATCTCAAATTTCCAGGAAGTATACCGGCAAGTCTCCAAGCAAAGCAGCTGATGTATCGGTTGCTGCATCGTGATCCTAAAAACAGATTGGGGTCTCTGGAAGGGGCAAACGAAATTAAGCGTCACCCTTTCTTTCGGGGTGTCAACTGGGCTCTAGTTCGCTGCATGAATCCTCCTAAACTTGATTCCCCTCTCTTCGGGACAACTGAAGCCGAAAAGGGTGCTAAGCTTGTCGATCCTGAGATGCAAGATCTACAAACAAATATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

1029

Amino Acids

115.24

Weight (kDa)

8.37

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 233 - 329 6e-20 PAS domain
PAS PF00989 238 - 324 7.7e-08 PAS fold
PAS_3 PF08447 239 - 323 1.5e-07 PAS fold
PAS_9 PF13426 525 - 617 7.6e-20 PAS domain
PAS PF00989 528 - 617 1.6e-07 PAS fold
PAS_3 PF08447 529 - 614 5.2e-07 PAS fold
Pkinase PF00069 705 - 990 2.8e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 706 - 852 3.4e-17 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 895 - 975 7.2e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AasI GACNNNNNNGTC 1 cut(s) 1446
AatII GACGTC 1 cut(s) 1413
Acc16I TGCGCA 1 cut(s) 402
AccB1I GGYRCC 2 cut(s) 239, 1231
AccBSI CCGCTC 2 cut(s) 782, 1326
AccI GTMKAC 3 cut(s) 641, 2712, 2836
AccII CGCG 6 cut(s) 66, 166, 231, 362, 1184, 1611
AccIII TCCGGA 2 cut(s) 546, 1945
AcoI YGGCCR 2 cut(s) 226, 2695
AcsI RAATTY 5 cut(s) 889, 938, 1138, 2641, 2822
AcuI CTGAAG 6 cut(s) 452, 1728, 1890, 2399, 2471, 3042
AcyI GRCGYC 2 cut(s) 1321, 1410
AdeI CACNNNGTG 2 cut(s) 1112, 1256
AfaI GTAC 9 cut(s) 517, 701, 734, 1604, 1719, 1803, 1821, 2162, 2432
AgsI TTSAA 8 cut(s) 85, 628, 721, 859, 1646, 2045, 2080, 2467
AhdI GACNNNNNGTC 2 cut(s) 13, 1125
AjiI CACGTC 1 cut(s) 802
AjnI CCWGG 4 cut(s) 143, 152, 2329, 2827
Alw21I GWGCWC 2 cut(s) 554, 564
Alw26I GTCTC 9 cut(s) 51, 440, 1090, 1155, 1432, 1649, 1901, 2852, 2913
AlwNI CAGNNNCTG 3 cut(s) 1073, 1658, 1670
Ama87I CYCGRG 3 cut(s) 491, 499, 607
Aor13HI TCCGGA 2 cut(s) 546, 1945
AoxI GGCC 4 cut(s) 226, 258, 408, 2695
ApoI RAATTY 5 cut(s) 889, 938, 1138, 2641, 2822
AspLEI GCGC 4 cut(s) 403, 710, 808, 1002
AsuC2I CCSGG 7 cut(s) 383, 451, 513, 556, 712, 730, 794
AsuHPI GGTGA 7 cut(s) 189, 238, 973, 1268, 2126, 2249, 2930
AsuII TTCGAA 1 cut(s) 1988
AvaI CYCGRG 3 cut(s) 491, 499, 607
AvaII GGWCC 8 cut(s) 488, 496, 790, 876, 931, 981, 1649, 2374
BalI TGGCCA 1 cut(s) 2697
BanI GGYRCC 2 cut(s) 239, 1231
BanII GRGCYC 4 cut(s) 554, 564, 2725, 2968
BarI GAAGNNNNNNTAC 2 cut(s) 1701, 1733
BauI CACGAG 1 cut(s) 835
BbsI GAAGAC 3 cut(s) 12, 440, 804
Bbv12I GWGCWC 2 cut(s) 554, 564
BbvCI CCTCAGC 1 cut(s) 812
BccI CCATC 7 cut(s) 215, 250, 251, 359, 866, 917, 1823
BceAI ACGGC 3 cut(s) 253, 286, 589
BcgI CGANNNNNNTGC 2 cut(s) 3017, 3051
BciT130I CCWGG 4 cut(s) 145, 154, 2331, 2829
BciVI GTATCC 1 cut(s) 778
BclI TGATCA 3 cut(s) 1786, 2155, 2313
BcnI CCSGG 7 cut(s) 383, 451, 513, 556, 712, 730, 794
BcoDI GTCTC 9 cut(s) 51, 440, 1090, 1155, 1432, 1649, 1901, 2852, 2913
BfaI CTAG 5 cut(s) 1239, 1317, 2054, 2703, 2969
BfmI CTRYAG 2 cut(s) 1772, 1911
BfoI RGCGCY 1 cut(s) 711
BfuI GTATCC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 2174
BglII AGATCT 1 cut(s) 3064
BlpI GCTNAGC 1 cut(s) 3039
BmcAI AGTACT 1 cut(s) 2162
Bme18I GGWCC 8 cut(s) 488, 496, 790, 876, 931, 981, 1649, 2374
BmeRI GACNNNNNGTC 2 cut(s) 13, 1125
BmeT110I CYCGRG 3 cut(s) 491, 499, 607
BmgBI CACGTC 1 cut(s) 802
BmrI ACTGGG 1 cut(s) 2971
BmuI ACTGGG 1 cut(s) 2971
BoxI GACNNNNGTC 2 cut(s) 501, 1102
BpiI GAAGAC 3 cut(s) 12, 440, 804
BplI GAGNNNNNCTC 4 cut(s) 1192, 1224, 2226, 2258
BpmI CTGGAG 3 cut(s) 1838, 2658, 2709
Bpu10I CCTNAGC 1 cut(s) 812
Bpu1102I GCTNAGC 1 cut(s) 3039
Bpu14I TTCGAA 1 cut(s) 1988
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 7 cut(s) 383, 451, 513, 556, 712, 730, 794
BsaHI GRCGYC 2 cut(s) 1321, 1410
BsaI GGTCTC 4 cut(s) 440, 1090, 1432, 2913
BsaWI WCCGGW 2 cut(s) 546, 1945
BsaXI ACNNNNNCTCC 4 cut(s) 889, 919, 1152, 1182
Bse118I RCCGGY 2 cut(s) 765, 2839
Bse1I ACTGG 6 cut(s) 928, 1902, 2125, 2692, 2720, 2966
Bse3DI GCAATG 1 cut(s) 2500
BseAI TCCGGA 2 cut(s) 546, 1945
BseBI CCWGG 4 cut(s) 145, 154, 2331, 2829
BseGI GGATG 7 cut(s) 207, 261, 676, 1574, 1954, 1983, 1999
BseMI GCAATG 1 cut(s) 2500
BseMII CTCAG 6 cut(s) 803, 966, 1065, 1209, 1395, 3045
BseNI ACTGG 6 cut(s) 928, 1902, 2125, 2692, 2720, 2966
BseRI GAGGAG 2 cut(s) 82, 1108
BseX3I CGGCCG 1 cut(s) 226
BseYI CCCAGC 2 cut(s) 28, 315
BsgI GTGCAG 2 cut(s) 1103, 2727
Bsh1236I CGCG 6 cut(s) 66, 166, 231, 362, 1184, 1611
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 4 cut(s) 228, 260, 410, 2697
BshNI GGYRCC 2 cut(s) 239, 1231
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 2 cut(s) 554, 564
BsiHKCI CYCGRG 3 cut(s) 491, 499, 607
BslFI GGGAC 5 cut(s) 298, 307, 889, 1113, 3028
BsmAI GTCTC 9 cut(s) 51, 440, 1090, 1155, 1432, 1649, 1901, 2852, 2913
BsmBI CGTCTC 1 cut(s) 1155
BsmFI GGGAC 5 cut(s) 298, 307, 889, 1113, 3028
BsmI GAATGC 1 cut(s) 954
BsnI GGCC 4 cut(s) 228, 260, 410, 2697
Bso31I GGTCTC 4 cut(s) 440, 1090, 1432, 2913
BsoBI CYCGRG 3 cut(s) 491, 499, 607
Bsp119I TTCGAA 1 cut(s) 1988
Bsp1286I GDGCHC 4 cut(s) 554, 564, 2725, 2968
Bsp13I TCCGGA 2 cut(s) 546, 1945
Bsp1720I GCTNAGC 1 cut(s) 3039
BspANI GGCC 4 cut(s) 228, 260, 410, 2697
BspCNI CTCAG 6 cut(s) 804, 967, 1066, 1208, 1396, 3046
BspEI TCCGGA 2 cut(s) 546, 1945
BspFNI CGCG 6 cut(s) 66, 166, 231, 362, 1184, 1611
BspMAI CTGCAG 2 cut(s) 1776, 1915
BspT104I TTCGAA 1 cut(s) 1988
BspT107I GGYRCC 2 cut(s) 239, 1231
BspTNI GGTCTC 4 cut(s) 440, 1090, 1432, 2913
BsrBI CCGCTC 2 cut(s) 782, 1326
BsrDI GCAATG 1 cut(s) 2500
BsrFI RCCGGY 2 cut(s) 765, 2839
BsrI ACTGG 6 cut(s) 928, 1902, 2125, 2692, 2720, 2966
BssAI RCCGGY 2 cut(s) 765, 2839
BssNAI GTATAC 1 cut(s) 2837
BssNI GRCGYC 2 cut(s) 1321, 1410
BssSI CACGAG 1 cut(s) 835
BssT1I CCWWGG 6 cut(s) 741, 984, 1544, 2030, 2103, 2424
Bst1107I GTATAC 1 cut(s) 2837
Bst2BI CACGAG 1 cut(s) 835
Bst2UI CCWGG 4 cut(s) 145, 154, 2331, 2829
Bst4CI ACNGT 9 cut(s) 15, 312, 1424, 1672, 1717, 1806, 2060, 2360, 2766
Bst6I CTCTTC 3 cut(s) 2348, 2655, 3014
BstACI GRCGYC 2 cut(s) 1321, 1410
BstAPI GCANNNNNTGC 3 cut(s) 1780, 2137, 2279
BstBI TTCGAA 1 cut(s) 1988
BstC8I GCNNGC 6 cut(s) 109, 113, 706, 968, 1086, 2695
BstDEI CTNAG 9 cut(s) 812, 975, 1074, 1109, 1195, 1404, 1588, 3039, 3054
BstDSI CCRYGG 3 cut(s) 64, 360, 1227
BstF5I GGATG 7 cut(s) 207, 261, 676, 1574, 1954, 1983, 1999
BstFNI CGCG 6 cut(s) 66, 166, 231, 362, 1184, 1611
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 4 cut(s) 403, 710, 808, 1002
BstMAI GTCTC 9 cut(s) 51, 440, 1090, 1155, 1432, 1649, 1901, 2852, 2913
BstMCI CGRYCG 1 cut(s) 229
BstNI CCWGG 4 cut(s) 145, 154, 2331, 2829
BstNSI RCATGY 2 cut(s) 2306, 2504
BstPAI GACNNNNGTC 2 cut(s) 501, 1102
BstSFI CTRYAG 2 cut(s) 1772, 1911
BstUI CGCG 6 cut(s) 66, 166, 231, 362, 1184, 1611
BstV2I GAAGAC 3 cut(s) 12, 440, 804
BstX2I RGATCY 5 cut(s) 1492, 2108, 2238, 2815, 3064
BstYI RGATCY 5 cut(s) 1492, 2108, 2238, 2815, 3064
BstZ17I GTATAC 1 cut(s) 2837
BstZI CGGCCG 1 cut(s) 226
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 4 cut(s) 228, 260, 410, 2697
BtgI CCRYGG 3 cut(s) 64, 360, 1227
BtrI CACGTC 1 cut(s) 802
BtsCI GGATG 7 cut(s) 207, 261, 676, 1574, 1954, 1983, 1999
BtsI GCAGTG 3 cut(s) 1095, 1920, 2131
BtsIMutI CAGTG 8 cut(s) 972, 1095, 1429, 1920, 2065, 2131, 2316, 2397
Cac8I GCNNGC 6 cut(s) 109, 113, 706, 968, 1086, 2695
CaiI CAGNNNCTG 3 cut(s) 1073, 1658, 1670
CfoI GCGC 4 cut(s) 403, 710, 808, 1002
Cfr10I RCCGGY 2 cut(s) 765, 2839
Cfr42I CCGCGG 2 cut(s) 67, 363
CseI GACGC 3 cut(s) 404, 1329, 2924
Csp6I GTAC 9 cut(s) 516, 700, 733, 1603, 1718, 1802, 1820, 2161, 2431
CviQI GTAC 9 cut(s) 516, 700, 733, 1603, 1718, 1802, 1820, 2161, 2431
DdeI CTNAG 9 cut(s) 812, 975, 1074, 1109, 1195, 1404, 1588, 3039, 3054
DraIII CACNNNGTG 2 cut(s) 1112, 1256
DrdI GACNNNNNNGTC 1 cut(s) 1446
DriI GACNNNNNGTC 2 cut(s) 13, 1125
DseDI GACNNNNNNGTC 1 cut(s) 1446
EaeI YGGCCR 2 cut(s) 226, 2695
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 3 cut(s) 2348, 2655, 3014
Eam1105I GACNNNNNGTC 2 cut(s) 13, 1125
EarI CTCTTC 3 cut(s) 2348, 2655, 3014
EciI GGCGGA 4 cut(s) 273, 346, 501, 600
Ecl136II GAGCTC 2 cut(s) 552, 562
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 6 cut(s) 741, 984, 1544, 2030, 2103, 2424
Eco24I GRGCYC 4 cut(s) 554, 564, 2725, 2968
Eco31I GGTCTC 4 cut(s) 440, 1090, 1432, 2913
Eco47I GGWCC 8 cut(s) 488, 496, 790, 876, 931, 981, 1649, 2374
Eco52I CGGCCG 1 cut(s) 226
Eco53kI GAGCTC 2 cut(s) 552, 562
Eco57I CTGAAG 6 cut(s) 452, 1728, 1890, 2399, 2471, 3042
Eco88I CYCGRG 3 cut(s) 491, 499, 607
EcoICRI GAGCTC 2 cut(s) 552, 562
EcoO109I RGGNCCY 4 cut(s) 496, 876, 931, 2374
EcoRII CCWGG 4 cut(s) 143, 152, 2329, 2827
EcoT14I CCWWGG 6 cut(s) 741, 984, 1544, 2030, 2103, 2424
EcoT22I ATGCAT 1 cut(s) 1278
EcoT38I GRGCYC 4 cut(s) 554, 564, 2725, 2968
ErhI CCWWGG 6 cut(s) 741, 984, 1544, 2030, 2103, 2424
Esp3I CGTCTC 1 cut(s) 1155
FaqI GGGAC 5 cut(s) 298, 307, 889, 1113, 3028
FauI CCCGC 7 cut(s) 71, 112, 120, 145, 171, 220, 333
FbaI TGATCA 3 cut(s) 1786, 2155, 2313
FblI GTMKAC 3 cut(s) 641, 2712, 2836
FokI GGATG 7 cut(s) 194, 268, 683, 1561, 1961, 1986, 1990
FriOI GRGCYC 4 cut(s) 554, 564, 2725, 2968
FspBI CTAG 5 cut(s) 1239, 1317, 2054, 2703, 2969
FspI TGCGCA 1 cut(s) 402
GlaI GCGC 4 cut(s) 402, 709, 807, 1001
GsaI CCCAGC 2 cut(s) 32, 319
GsuI CTGGAG 3 cut(s) 1838, 2658, 2709
HaeII RGCGCY 1 cut(s) 711
HaeIII GGCC 4 cut(s) 228, 260, 410, 2697
HgaI GACGC 3 cut(s) 404, 1329, 2924
HhaI GCGC 4 cut(s) 403, 710, 808, 1002
Hin1I GRCGYC 2 cut(s) 1321, 1410
Hin6I GCGC 4 cut(s) 401, 708, 806, 1000
HinP1I GCGC 4 cut(s) 401, 708, 806, 1000
HincII GTYRAC 6 cut(s) 367, 378, 642, 2363, 2600, 2959
HindII GTYRAC 6 cut(s) 367, 378, 642, 2363, 2600, 2959
HindIII AAGCTT 1 cut(s) 3041
HphI GGTGA 7 cut(s) 189, 238, 973, 1268, 2126, 2249, 2930
Hpy166II GTNNAC 9 cut(s) 367, 378, 642, 735, 2363, 2600, 2713, 2837, 2959
Hpy8I GTNNAC 9 cut(s) 367, 378, 642, 735, 2363, 2600, 2713, 2837, 2959
Hpy99I CGWCG 8 cut(s) 19, 236, 239, 398, 523, 572, 575, 646
HpyCH4III ACNGT 9 cut(s) 15, 312, 1424, 1672, 1717, 1806, 2060, 2360, 2766
HpyCH4IV ACGT 9 cut(s) 518, 633, 644, 656, 801, 1243, 1410, 1513, 2596
HpyF3I CTNAG 9 cut(s) 812, 975, 1074, 1109, 1195, 1404, 1588, 3039, 3054
HpySE526I ACGT 9 cut(s) 518, 633, 644, 656, 801, 1243, 1410, 1513, 2596
Hsp92I GRCGYC 2 cut(s) 1321, 1410
HspAI GCGC 4 cut(s) 401, 708, 806, 1000
KflI GGGWCCC 1 cut(s) 876
Kpn2I TCCGGA 2 cut(s) 546, 1945
Ksp22I TGATCA 3 cut(s) 1786, 2155, 2313
KspI CCGCGG 2 cut(s) 67, 363
MaeI CTAG 5 cut(s) 1239, 1317, 2054, 2703, 2969
MaeII ACGT 9 cut(s) 518, 633, 644, 656, 801, 1243, 1410, 1513, 2596
MbiI CCGCTC 2 cut(s) 782, 1326
MfeI CAATTG 2 cut(s) 1692, 2141
MflI RGATCY 5 cut(s) 1492, 2108, 2238, 2815, 3064
MhlI GDGCHC 4 cut(s) 554, 564, 2725, 2968
MlsI TGGCCA 1 cut(s) 2697
MluNI TGGCCA 1 cut(s) 2697
MlyI GAGTC 4 cut(s) 53, 511, 1232, 1456
MmeI TCCRAC 7 cut(s) 302, 558, 591, 648, 1602, 2232, 2588
Mox20I TGGCCA 1 cut(s) 2697
Mph1103I ATGCAT 1 cut(s) 1278
MroI TCCGGA 2 cut(s) 546, 1945
MscI TGGCCA 1 cut(s) 2697
MseI TTAA 3 cut(s) 2088, 2571, 2931
MslI CAYNNNNRTG 2 cut(s) 947, 2703
Msp20I TGGCCA 1 cut(s) 2697
MspA1I CMGCKG 4 cut(s) 66, 362, 811, 2863
MunI CAATTG 2 cut(s) 1692, 2141
Mva1269I GAATGC 1 cut(s) 954
MvaI CCWGG 4 cut(s) 145, 154, 2331, 2829
MvnI CGCG 6 cut(s) 66, 166, 231, 362, 1184, 1611
NciI CCSGG 7 cut(s) 383, 451, 513, 556, 712, 730, 794
NmeAIII GCCGAG 1 cut(s) 439
NmuCI GTSAC 9 cut(s) 167, 195, 466, 503, 1104, 1415, 1424, 1711, 2936
NsbI TGCGCA 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 1278
NspI RCATGY 2 cut(s) 2306, 2504
NspV TTCGAA 1 cut(s) 1988
PcsI WCGNNNNNNNCGW 1 cut(s) 1416
PctI GAATGC 1 cut(s) 954
PfeI GAWTC 9 cut(s) 591, 1019, 1115, 1638, 1981, 2050, 2387, 2983, 2999
PfoI TCCNGGA 1 cut(s) 2827
PleI GAGTC 4 cut(s) 53, 510, 1231, 1455
PpsI GAGTC 4 cut(s) 53, 510, 1231, 1455
PpuMI RGGWCCY 4 cut(s) 496, 876, 931, 2374
PshAI GACNNNNGTC 2 cut(s) 501, 1102
PsiI TTATAA 1 cut(s) 864
Psp124BI GAGCTC 2 cut(s) 554, 564
Psp5II RGGWCCY 4 cut(s) 496, 876, 931, 2374
Psp6I CCWGG 4 cut(s) 143, 152, 2329, 2827
PspFI CCCAGC 2 cut(s) 28, 315
PspGI CCWGG 4 cut(s) 143, 152, 2329, 2827
PspPPI RGGWCCY 4 cut(s) 496, 876, 931, 2374
PsrI GAACNNNNNNTAC 2 cut(s) 2331, 2363
PstI CTGCAG 2 cut(s) 1776, 1915
PstNI CAGNNNCTG 3 cut(s) 1073, 1658, 1670
PsuI RGATCY 5 cut(s) 1492, 2108, 2238, 2815, 3064
PvuII CAGCTG 2 cut(s) 811, 2863
RsaI GTAC 9 cut(s) 517, 701, 734, 1604, 1719, 1803, 1821, 2162, 2432
RsaNI GTAC 9 cut(s) 516, 700, 733, 1603, 1718, 1802, 1820, 2161, 2431
RseI CAYNNNNRTG 2 cut(s) 947, 2703
SacI GAGCTC 2 cut(s) 554, 564
SacII CCGCGG 2 cut(s) 67, 363
SalI GTCGAC 1 cut(s) 640
SaqAI TTAA 3 cut(s) 2088, 2571, 2931
ScaI AGTACT 1 cut(s) 2162
SchI GAGTC 4 cut(s) 53, 511, 1232, 1456
SduI GDGCHC 4 cut(s) 554, 564, 2725, 2968
SfcI CTRYAG 2 cut(s) 1772, 1911
Sfr303I CCGCGG 2 cut(s) 67, 363
SfuI TTCGAA 1 cut(s) 1988
SgrBI CCGCGG 2 cut(s) 67, 363
SinI GGWCC 8 cut(s) 488, 496, 790, 876, 931, 981, 1649, 2374
SmiMI CAYNNNNRTG 2 cut(s) 947, 2703
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SspI AATATT 3 cut(s) 1620, 2803, 3079
SspMI CTAG 5 cut(s) 1239, 1317, 2054, 2703, 2969
SstI GAGCTC 2 cut(s) 554, 564
StyI CCWWGG 6 cut(s) 741, 984, 1544, 2030, 2103, 2424
TaaI ACNGT 9 cut(s) 15, 312, 1424, 1672, 1717, 1806, 2060, 2360, 2766
TaiI ACGT 9 cut(s) 521, 636, 647, 659, 804, 1246, 1413, 1516, 2599
TaqII GACCGA 2 cut(s) 1114, 2528
TatI WGTACW 4 cut(s) 1602, 1801, 1819, 2160
TfiI GAWTC 9 cut(s) 591, 1019, 1115, 1638, 1981, 2050, 2387, 2983, 2999
Tru1I TTAA 3 cut(s) 2088, 2571, 2931
Tru9I TTAA 3 cut(s) 2088, 2571, 2931
TscAI CASTG 8 cut(s) 979, 1095, 1429, 1920, 2065, 2131, 2323, 2397
TseFI GTSAC 9 cut(s) 167, 195, 466, 503, 1104, 1415, 1424, 1711, 2936
Tsp45I GTSAC 9 cut(s) 167, 195, 466, 503, 1104, 1415, 1424, 1711, 2936
TspGWI ACGGA 6 cut(s) 452, 803, 1216, 1403, 1613, 2672
TspRI CASTG 8 cut(s) 979, 1095, 1429, 1920, 2065, 2131, 2323, 2397
VpaK11BI GGWCC 8 cut(s) 488, 496, 790, 876, 931, 981, 1649, 2374
XapI RAATTY 5 cut(s) 889, 938, 1138, 2641, 2822
XbaI TCTAGA 1 cut(s) 2053
XceI RCATGY 2 cut(s) 2306, 2504
XcmI CCANNNNNNNNNTGG 2 cut(s) 1971, 2176
XmiI GTMKAC 3 cut(s) 641, 2712, 2836
XspI CTAG 5 cut(s) 1239, 1317, 2054, 2703, 2969
ZraI GACGTC 1 cut(s) 1411
ZrmI AGTACT 1 cut(s) 2162
Zsp2I ATGCAT 1 cut(s) 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.