MD11G1119700.v1.1

PAS fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004648
Physical Location & Seq
Reverse (-)
10893953 .. 10906157
12205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1119700.v1.1.491

Sequence Viewer

Length: 3075 bp
ATGGAGGGATGGGAGGCCAAACCTTTTATGGAAGACGAACAGTTCTCAGACACAACGGCACCCGCCCTCATCCCGCCATTCCCAAGAGACTCCCGCGGCTCTCTCGAAGTCTTCAACCCCTCCACCTTCTCCACCGCCGCCAAACCAACAAAAACTACAACCGGTTCACCAGCCTTCCGCTCCCAGCAGACGTGGCAGAGCTGGACAGACCCACTTGCCACCACCCCGGAACCCGACCCATTACCCAACCTCGCCTCCAAGTCCACCCGCACCGCCGACATCACAACCTCATGGATGGCCCTCAAGGACGTCGCCCACCACTCCCCAACCCCATCTCCGCTGTCCAATATTCAGCAGACAATCTCCTCCTCCTCTGTCGATGCAAACGACAAGCCGTCCGACGACGCCGCGCAGAGGGCTGCCGAGTGGGGTCTGGTGCTGAAGACGGACGAGGAGACCGGGAAGCCGCAGGGAGTCGGCGTCCGGACCTCGGGCGGACCCGAAGACTCGACTTCCAAACCGAGTCTTGGCGGAGGAACTGAGGCCTCGAGAAGGAACTCGAACAACTCGACTCGGAGCTCCGGCGAGTTGTCGTCGGACGACGTGTTCGGCGGAGGGAAAGAGAGAGGGATTCCAAGAGTTTCGAATGACTTGAAAGACGCGCTGTCGACGTTTCAGCAGACGTTCGTCGTTTCGGACGCCACCAAACCCGATTACCCGATTCTTTACGCCAGCGCCGGGTTCTTTAAAATGACCGGGTACACCTCCAAGGAGGTCATCGGCAGGAACTGCCGGTTTTTGCAGGGGGCGGGTACGGACCCGCAAGATGTTGCACAGATAAGGGAGGCTTTGCAAGGGAACAAGAGTTACTGTGGGAGGTTGCTGAATTATAAGAAAGATGGGACCCCTTTTTGGAATTTACTCACTATTGCTCCCATCAAGGACGAGAGTGGGAAGGTTCTCAAATTCATTGGAATGCAAGTGGAAGTGAGCAAGCATACAGAGGGGTCCAAGGATAAGATGCTGCGTCCTAATGGATTGCCCGAATCCTTGATTCGATATGATGCTCGTCAGAAAGAGATGGCTTCAAACTCAGTATCTGAACTGGTACAGGCAGTGAAAAGACCAAGATCACTTAGCGAATCAGCAAATCGTCCCTTCTTTAGAAAGTCAGAAGGTGGCAGGGCAGAGGAGAGAACAGATCAGCCTCCAGCTAGGAGAAACTCTGAGAGTGTTGGTGTACCTAGGCGAAACTCACGCGGGGATCATCCAAAAATCTCAATGCAGCGTATCAACGAGTTGCCTGAAAAGAAACCGAAAAAAACTAGCCGGCTTTCTTTCATGGGGAGGATTAGGAAAAGCCAAACTATTGAGGAAAGCTTTGACACCGGAGTCCCTGAGGATAGCTATGAGAGTGACGAGGAGAGACCGGACAGTCTTGACGACAAAGTAAGACAGAAGGAAATGAGGAAGGGTATTGATCTTGCTACAACACTCGAACGTATTGAGAAAAACTTTGTCATTACTGATCCAAGGCTGCCTGATAATCCCATTATATTTGCGTCCGACAGTTTCTTAGAGCTTACAGAGTACAGCCGCGAAGAAATATTGGGAAGAAATTGCAGGTTCCTGCAAGGACCTGAAACTGATCCGGAAACCGTGAAGAAAATAAGAGATGCAATCAACAACCAGACTGAAGTTACCGTGCAGCTCATCAATTATTCGAAAAGCGGTAAAAAGTTCTGGAATGTATTTCATCTGCAGCCTATGCGTGATCAAAAGGGGGAGGTGCAGTATTTTATTGGGGTGCAACTAGATGGCAGTGAGCATATTGAGCCTCTTAAGAACGCTATTCCTGAAGATACCGTAAAAGAGAGCGAAAAACTGATCAGAGAAACTGCAGAAAATGTCGATGAGGCAGCGAGAGAACTTCCTGATGCCAATTTGAAACCAGAAGATCTGTGGATGAACCATTCGAAGGCTGTTCATCCAAAGCCTCATAGAAAATATACCCCCTCTTGGGTAGCTATCCAAAAGATTCTAGACAGTGGAGAGCCGATCGGATTGAAACATTTTAGGCCTATAAAACCCTTGGGATCTGGTGATACTGGCAATGTGCATTTGGTTGAACTGAGTGGAACCGGTCACTACTTCGCCATGAAGGCTATGGATAAGGGTGTCATGCTCAATCGCAACAAGGTGCACAGAGCTTGCGCAGAGAGAGAGATCCTCGACATATTGGATCACCCTTTTCTTCCTGCATTGTATGCTTCATTTCAGACCAAAACACATGTTTGCCTCATTACTGATTACTACCCGGGTGGAGAACTCTTCGTGCTTTTGGACAGACAACCGACGAAGGTTCTTAAGGAGGATTCTGCGAGATTTTACGTTGCAGAGGTAGTTGTTGCACTGGAGTATCTTCACTGTCAGGGGATAATATACAGGGATTTGAAGCCAGAAAATGTTTTACTTCAGAGCAATGGGCATGTGTCCCTGACAGATTTTGATCTATCGTGTTTAACATCTTGCAAACCACAGCTTCTACTTCCAAGCATCAAAGAAAAGAAAAAACAACGCAAATCTCAACAAAATATGATCTTCATGGCTGAACCAATGAGAGCATCAAATTCCTTCGTTGGAACTGAAGAGTACATAGCTCCGGAAATCATAACTGGAGCAGGTCATACCAGTGCAGTGGACTGGTGGGCTCTTGGTATTCTTCTGTATGAAATGCTCTATGGATATACACCATTCAGGGGGAAAACAAGGCAAAGGACGTTCGCCAATATTCTTCACAAGGATCTTAAATTTCCAGGAAGTATATCGGCAAGTCTCCAAGCAAAGCAGTTAATGTATCGGTTGTTGCACCGTGATCCCAAAAACAGATTGGGCTCACAGGAAGGAGCAGATGAAATTAAGCGCCATCCGTTCTTCAAGGGAATCAACTGGGCTCTTGTTCGCTGCATGAACCCTCCTCAACTCGATGCTCCCCTCTTTGCAAAAACTGAAGCCGAAAGGGGAGCAGATGCTGTAGATCCTGAGATGCAAGATTTACAAACAAACATATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

1025

Amino Acids

114.74

Weight (kDa)

6.9

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 235 - 331 1e-20 PAS domain
PAS_3 PF08447 241 - 325 3e-07 PAS fold
PAS PF00989 241 - 326 4e-07 PAS fold
PAS_9 PF13426 514 - 606 5.4e-20 PAS domain
PAS PF00989 517 - 606 2.6e-07 PAS fold
PAS_3 PF08447 518 - 603 6.3e-07 PAS fold
Pkinase PF00069 695 - 979 1.8e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 696 - 964 7.9e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 891
AasI GACNNNNNNGTC 2 cut(s) 1391, 1434
AatII GACGTC 1 cut(s) 312
Acc16I TGCGCA 1 cut(s) 2215
Acc36I ACCTGC 2 cut(s) 1614, 2672
AccB1I GGYRCC 1 cut(s) 58
AccBSI CCGCTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 668
AccII CGCG 5 cut(s) 96, 410, 662, 1260, 1599
AccIII TCCGGA 3 cut(s) 483, 1651, 2662
AclWI GGATC 9 cut(s) 1272, 1523, 1643, 2104, 2221, 2250, 2811, 2870, 3032
AcsI RAATTY 4 cut(s) 916, 965, 2629, 2810
AcuI CTGAAG 6 cut(s) 461, 1716, 1878, 2459, 2667, 3030
AcyI GRCGYC 4 cut(s) 309, 405, 480, 699
AfaI GTAC 6 cut(s) 761, 814, 1110, 1242, 1592, 2654
AflII CTTAAG 2 cut(s) 1841, 2366
AflIII ACRYGT 2 cut(s) 603, 2290
AgeI ACCGGT 2 cut(s) 161, 2141
AgsI TTSAA 8 cut(s) 115, 655, 1089, 1948, 2068, 2129, 2455, 2938
AhdI GACNNNNNGTC 2 cut(s) 394, 664
AjiI CACGTC 2 cut(s) 192, 604
AjnI CCWGG 1 cut(s) 2815
AloI GAACNNNNNNTCC 2 cut(s) 590, 622
Alw21I GWGCWC 2 cut(s) 581, 2205
Alw26I GTCTC 4 cut(s) 81, 449, 1420, 2840
Alw44I GTGCAC 1 cut(s) 2201
AlwI GGATC 9 cut(s) 1272, 1523, 1643, 2104, 2221, 2250, 2811, 2870, 3032
AlwNI CAGNNNCTG 3 cut(s) 789, 1100, 3032
Ama87I CYCGRG 3 cut(s) 490, 547, 2317
Aor13HI TCCGGA 3 cut(s) 483, 1651, 2662
AoxI GGCC 4 cut(s) 15, 297, 543, 2078
ApaLI GTGCAC 1 cut(s) 2201
ApeKI GCWGC 8 cut(s) 419, 1024, 1285, 1537, 1708, 1762, 1919, 2964
ApoI RAATTY 4 cut(s) 916, 965, 2629, 2810
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
AsiGI ACCGGT 2 cut(s) 161, 2141
AspA2I CCTAGG 1 cut(s) 1244
AspLEI GCGC 5 cut(s) 412, 664, 737, 2216, 2925
AspS9I GGNCC 7 cut(s) 298, 486, 497, 817, 903, 1008, 1637
AsuC2I CCSGG 6 cut(s) 227, 460, 739, 757, 2318, 2319
AsuHPI GGTGA 3 cut(s) 159, 2114, 2237
AsuII TTCGAA 3 cut(s) 644, 1724, 1976
AvaI CYCGRG 3 cut(s) 490, 547, 2317
AvaII GGWCC 6 cut(s) 486, 497, 817, 903, 1008, 1637
AvrII CCTAGG 1 cut(s) 1244
AxyI CCTNAGG 1 cut(s) 1398
BaeGI GKGCMC 1 cut(s) 2205
BaeI ACNNNNGTAYC 2 cut(s) 2402, 2435
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 4 cut(s) 581, 2713, 2897, 2956
BbsI GAAGAC 4 cut(s) 39, 103, 449, 510
Bbv12I GWGCWC 2 cut(s) 581, 2205
BbvI GCAGC 8 cut(s) 406, 1011, 1297, 1524, 1720, 1774, 1931, 2951
BccI CCATC 8 cut(s) 3, 289, 340, 893, 944, 1075, 1811, 2934
BceAI ACGGC 2 cut(s) 72, 379
BciT130I CCWGG 1 cut(s) 2817
BclI TGATCA 2 cut(s) 1774, 1887
BcnI CCSGG 6 cut(s) 227, 460, 739, 757, 2318, 2319
BcoDI GTCTC 4 cut(s) 81, 449, 1420, 2840
BfaI CTAG 5 cut(s) 1215, 1245, 1326, 1814, 2042
BfmI CTRYAG 3 cut(s) 1760, 1899, 3033
BfoI RGCGCY 2 cut(s) 738, 2926
BfrI CTTAAG 2 cut(s) 1841, 2366
BfuAI ACCTGC 2 cut(s) 1614, 2672
BglI GCCNNNNNGGC 1 cut(s) 2162
BglII AGATCT 1 cut(s) 1957
BlnI CCTAGG 1 cut(s) 1244
Bme1390I CCNGG 7 cut(s) 227, 460, 739, 757, 2318, 2319, 2817
Bme18I GGWCC 6 cut(s) 486, 497, 817, 903, 1008, 1637
BmeRI GACNNNNNGTC 2 cut(s) 394, 664
BmeT110I CYCGRG 3 cut(s) 490, 547, 2317
BmgBI CACGTC 2 cut(s) 192, 604
BmgT120I GGNCC 7 cut(s) 298, 486, 497, 817, 903, 1008, 1637
BmiI GGNNCC 9 cut(s) 60, 231, 499, 819, 904, 905, 1009, 1628, 2140
BmrFI CCNGG 7 cut(s) 227, 460, 739, 757, 2318, 2319, 2817
BmrI ACTGGG 1 cut(s) 2959
BmuI ACTGGG 1 cut(s) 2959
BoxI GACNNNNGTC 1 cut(s) 686
BpiI GAAGAC 4 cut(s) 39, 103, 449, 510
BplI GAGNNNNNCTC 2 cut(s) 2214, 2246
BpmI CTGGAG 3 cut(s) 1194, 2435, 2697
Bpu14I TTCGAA 3 cut(s) 644, 1724, 1976
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 6 cut(s) 227, 460, 739, 757, 2318, 2319
BsaBI GATNNNNATC 1 cut(s) 2508
BsaHI GRCGYC 4 cut(s) 309, 405, 480, 699
BsaI GGTCTC 2 cut(s) 449, 1420
BsaJI CCNNGG 9 cut(s) 94, 225, 489, 768, 1011, 1244, 1532, 2091, 2317
BsaWI WCCGGW 7 cut(s) 161, 483, 1388, 1429, 1651, 2141, 2662
Bse118I RCCGGY 4 cut(s) 161, 792, 1329, 2141
Bse1I ACTGG 7 cut(s) 1110, 2113, 2418, 2680, 2691, 2708, 2954
Bse21I CCTNAGG 1 cut(s) 1398
Bse3DI GCAATG 2 cut(s) 2119, 2488
Bse8I GATNNNNATC 1 cut(s) 2508
BseAI TCCGGA 3 cut(s) 483, 1651, 2662
BseBI CCWGG 1 cut(s) 2817
BseDI CCNNGG 9 cut(s) 94, 225, 489, 768, 1011, 1244, 1532, 2091, 2317
BseGI GGATG 7 cut(s) 14, 69, 300, 1267, 1971, 1987, 2926
BseJI GATNNNNATC 1 cut(s) 2508
BseMI GCAATG 2 cut(s) 2119, 2488
BseMII CTCAG 7 cut(s) 60, 531, 1107, 1218, 1389, 2123, 3033
BseNI ACTGG 7 cut(s) 1110, 2113, 2418, 2680, 2691, 2708, 2954
BseRI GAGGAG 7 cut(s) 355, 358, 361, 467, 1205, 1436, 2967
BseSI GKGCMC 1 cut(s) 2205
BseXI GCAGC 8 cut(s) 406, 1011, 1297, 1524, 1720, 1774, 1931, 2951
BseYI CCCAGC 1 cut(s) 183
BsgI GTGCAG 3 cut(s) 1727, 1811, 2715
Bsh1236I CGCG 5 cut(s) 96, 410, 662, 1260, 1599
Bsh1285I CGRYCG 1 cut(s) 2061
BshFI GGCC 4 cut(s) 17, 299, 545, 2080
BshNI GGYRCC 1 cut(s) 58
BshTI ACCGGT 2 cut(s) 161, 2141
BsiEI CGRYCG 1 cut(s) 2061
BsiHKAI GWGCWC 2 cut(s) 581, 2205
BsiHKCI CYCGRG 3 cut(s) 490, 547, 2317
BslFI GGGAC 4 cut(s) 916, 1140, 1379, 2479
BsmAI GTCTC 4 cut(s) 81, 449, 1420, 2840
BsmFI GGGAC 4 cut(s) 916, 1140, 1379, 2479
BsmI GAATGC 1 cut(s) 981
BsnI GGCC 4 cut(s) 17, 299, 545, 2080
Bso31I GGTCTC 2 cut(s) 449, 1420
BsoBI CYCGRG 3 cut(s) 490, 547, 2317
Bsp119I TTCGAA 3 cut(s) 644, 1724, 1976
Bsp1286I GDGCHC 5 cut(s) 581, 2205, 2713, 2897, 2956
Bsp13I TCCGGA 3 cut(s) 483, 1651, 2662
BspANI GGCC 4 cut(s) 17, 299, 545, 2080
BspCNI CTCAG 7 cut(s) 59, 532, 1106, 1219, 1390, 2124, 3034
BspEI TCCGGA 3 cut(s) 483, 1651, 2662
BspFNI CGCG 5 cut(s) 96, 410, 662, 1260, 1599
BspLI GGNNCC 9 cut(s) 60, 231, 499, 819, 904, 905, 1009, 1628, 2140
BspMAI CTGCAG 2 cut(s) 1764, 1903
BspMI ACCTGC 2 cut(s) 1614, 2672
BspPI GGATC 9 cut(s) 1272, 1523, 1643, 2104, 2221, 2250, 2811, 2870, 3032
BspT104I TTCGAA 3 cut(s) 644, 1724, 1976
BspT107I GGYRCC 1 cut(s) 58
BspTI CTTAAG 2 cut(s) 1841, 2366
BspTNI GGTCTC 2 cut(s) 449, 1420
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 2 cut(s) 2119, 2488
BsrFI RCCGGY 4 cut(s) 161, 792, 1329, 2141
BsrI ACTGG 7 cut(s) 1110, 2113, 2418, 2680, 2691, 2708, 2954
BssAI RCCGGY 4 cut(s) 161, 792, 1329, 2141
BssECI CCNNGG 9 cut(s) 94, 225, 489, 768, 1011, 1244, 1532, 2091, 2317
BssNI GRCGYC 4 cut(s) 309, 405, 480, 699
BssT1I CCWWGG 5 cut(s) 768, 1011, 1244, 1532, 2091
Bst2UI CCWGG 1 cut(s) 2817
Bst6I CTCTTC 2 cut(s) 2336, 2643
BstACI GRCGYC 4 cut(s) 309, 405, 480, 699
BstAFI CTTAAG 2 cut(s) 1841, 2366
BstAPI GCANNNNNTGC 3 cut(s) 789, 1768, 2267
BstBI TTCGAA 3 cut(s) 644, 1724, 1976
BstC8I GCNNGC 4 cut(s) 733, 995, 1331, 2212
BstDEI CTNAG 9 cut(s) 46, 540, 1093, 1136, 1227, 1398, 1576, 2132, 3042
BstDSI CCRYGG 1 cut(s) 94
BstENI CCTNNNNNAGG 1 cut(s) 550
BstF5I GGATG 7 cut(s) 14, 69, 300, 1267, 1971, 1987, 2926
BstFNI CGCG 5 cut(s) 96, 410, 662, 1260, 1599
BstH2I RGCGCY 2 cut(s) 738, 2926
BstHHI GCGC 5 cut(s) 412, 664, 737, 2216, 2925
BstMAI GTCTC 4 cut(s) 81, 449, 1420, 2840
BstMCI CGRYCG 1 cut(s) 2061
BstMWI GCNNNNNNNGC 7 cut(s) 193, 416, 789, 1768, 1834, 2162, 2267
BstNI CCWGG 1 cut(s) 2817
BstNSI RCATGY 2 cut(s) 2294, 2492
BstPAI GACNNNNGTC 1 cut(s) 686
BstSCI CCNGG 7 cut(s) 225, 458, 737, 755, 2316, 2317, 2815
BstSFI CTRYAG 3 cut(s) 1760, 1899, 3033
BstSLI GKGCMC 1 cut(s) 2205
BstUI CGCG 5 cut(s) 96, 410, 662, 1260, 1599
BstV1I GCAGC 8 cut(s) 406, 1011, 1297, 1524, 1720, 1774, 1931, 2951
BstV2I GAAGAC 4 cut(s) 39, 103, 449, 510
BstX2I RGATCY 5 cut(s) 1957, 2096, 2226, 2803, 3037
BstXI CCANNNNNNTGG 1 cut(s) 2698
BstYI RGATCY 5 cut(s) 1957, 2096, 2226, 2803, 3037
Bsu36I CCTNAGG 1 cut(s) 1398
BsuRI GGCC 4 cut(s) 17, 299, 545, 2080
BtgI CCRYGG 1 cut(s) 94
BtrI CACGTC 2 cut(s) 192, 604
BtsCI GGATG 7 cut(s) 14, 69, 300, 1267, 1971, 1987, 2926
BtsI GCAGTG 3 cut(s) 1122, 1828, 2703
BtsIMutI CAGTG 7 cut(s) 1122, 1828, 2053, 2411, 2425, 2698, 2703
BveI ACCTGC 2 cut(s) 1614, 2672
Cac8I GCNNGC 4 cut(s) 733, 995, 1331, 2212
CaiI CAGNNNCTG 3 cut(s) 789, 1100, 3032
CfoI GCGC 5 cut(s) 412, 664, 737, 2216, 2925
Cfr10I RCCGGY 4 cut(s) 161, 792, 1329, 2141
Cfr13I GGNCC 7 cut(s) 298, 486, 497, 817, 903, 1008, 1637
Cfr42I CCGCGG 1 cut(s) 97
Cfr9I CCCGGG 1 cut(s) 2317
CseI GACGC 6 cut(s) 413, 469, 668, 707, 1016, 1551
Csp6I GTAC 6 cut(s) 760, 813, 1109, 1241, 1591, 2653
CspAI ACCGGT 2 cut(s) 161, 2141
CviAII CATG 8 cut(s) 291, 1342, 2158, 2182, 2291, 2489, 2605, 2968
CviQI GTAC 6 cut(s) 760, 813, 1109, 1241, 1591, 2653
DdeI CTNAG 9 cut(s) 46, 540, 1093, 1136, 1227, 1398, 1576, 2132, 3042
DraI TTTAAA 1 cut(s) 748
DrdI GACNNNNNNGTC 2 cut(s) 1391, 1434
DriI GACNNNNNGTC 2 cut(s) 394, 664
DseDI GACNNNNNNGTC 2 cut(s) 1391, 1434
Eam1104I CTCTTC 2 cut(s) 2336, 2643
Eam1105I GACNNNNNGTC 2 cut(s) 394, 664
EarI CTCTTC 2 cut(s) 2336, 2643
EciI GGCGGA 3 cut(s) 510, 546, 627
Ecl136II GAGCTC 1 cut(s) 579
Eco130I CCWWGG 5 cut(s) 768, 1011, 1244, 1532, 2091
Eco147I AGGCCT 2 cut(s) 545, 2080
Eco24I GRGCYC 4 cut(s) 581, 2713, 2897, 2956
Eco31I GGTCTC 2 cut(s) 449, 1420
Eco47I GGWCC 6 cut(s) 486, 497, 817, 903, 1008, 1637
Eco53kI GAGCTC 1 cut(s) 579
Eco57I CTGAAG 6 cut(s) 461, 1716, 1878, 2459, 2667, 3030
Eco81I CCTNAGG 1 cut(s) 1398
Eco88I CYCGRG 3 cut(s) 490, 547, 2317
EcoICRI GAGCTC 1 cut(s) 579
EcoNI CCTNNNNNAGG 1 cut(s) 550
EcoO109I RGGNCCY 2 cut(s) 903, 1637
EcoRII CCWGG 1 cut(s) 2815
EcoT14I CCWWGG 5 cut(s) 768, 1011, 1244, 1532, 2091
EcoT38I GRGCYC 4 cut(s) 581, 2713, 2897, 2956
ErhI CCWWGG 5 cut(s) 768, 1011, 1244, 1532, 2091
FaeI CATG 8 cut(s) 294, 1345, 2161, 2185, 2294, 2492, 2608, 2971
FalI AAGNNNNNCTT 2 cut(s) 832, 864
FaqI GGGAC 4 cut(s) 916, 1140, 1379, 2479
FatI CATG 8 cut(s) 290, 1341, 2157, 2181, 2290, 2488, 2604, 2967
FauI CCCGC 7 cut(s) 70, 81, 101, 275, 802, 828, 1253
FbaI TGATCA 2 cut(s) 1774, 1887
FblI GTMKAC 1 cut(s) 668
FokI GGATG 7 cut(s) 21, 56, 307, 1254, 1974, 1978, 2913
FriOI GRGCYC 4 cut(s) 581, 2713, 2897, 2956
FspBI CTAG 5 cut(s) 1215, 1245, 1326, 1814, 2042
FspI TGCGCA 1 cut(s) 2215
GlaI GCGC 5 cut(s) 411, 663, 736, 2215, 2924
GsaI CCCAGC 1 cut(s) 187
GsuI CTGGAG 3 cut(s) 1194, 2435, 2697
HaeII RGCGCY 2 cut(s) 738, 2926
HaeIII GGCC 4 cut(s) 17, 299, 545, 2080
HgaI GACGC 6 cut(s) 413, 469, 668, 707, 1016, 1551
HhaI GCGC 5 cut(s) 412, 664, 737, 2216, 2925
Hin1I GRCGYC 4 cut(s) 309, 405, 480, 699
Hin1II CATG 8 cut(s) 294, 1345, 2161, 2185, 2294, 2492, 2608, 2971
Hin6I GCGC 5 cut(s) 410, 662, 735, 2214, 2923
HinP1I GCGC 5 cut(s) 410, 662, 735, 2214, 2923
HincII GTYRAC 1 cut(s) 669
HindII GTYRAC 1 cut(s) 669
HindIII AAGCTT 1 cut(s) 1378
HphI GGTGA 3 cut(s) 159, 2114, 2237
Hpy166II GTNNAC 7 cut(s) 167, 264, 669, 762, 1241, 2203, 2701
Hpy8I GTNNAC 7 cut(s) 167, 264, 669, 762, 1241, 2203, 2701
Hpy99I CGWCG 8 cut(s) 314, 404, 407, 598, 605, 673, 692, 2359
HpyCH4IV ACGT 8 cut(s) 191, 309, 603, 671, 683, 1501, 2391, 2780
HpyF10VI GCNNNNNNNGC 7 cut(s) 193, 416, 789, 1768, 1834, 2162, 2267
HpyF3I CTNAG 9 cut(s) 46, 540, 1093, 1136, 1227, 1398, 1576, 2132, 3042
HpySE526I ACGT 8 cut(s) 191, 309, 603, 671, 683, 1501, 2391, 2780
Hsp92I GRCGYC 4 cut(s) 309, 405, 480, 699
Hsp92II CATG 8 cut(s) 294, 1345, 2161, 2185, 2294, 2492, 2608, 2971
HspAI GCGC 5 cut(s) 410, 662, 735, 2214, 2923
KflI GGGWCCC 1 cut(s) 903
Kpn2I TCCGGA 3 cut(s) 483, 1651, 2662
KroI GCCGGC 1 cut(s) 1329
KroNI GCCGGC 1 cut(s) 1331
Ksp22I TGATCA 2 cut(s) 1774, 1887
KspI CCGCGG 1 cut(s) 97
LmnI GCTCC 9 cut(s) 185, 576, 584, 937, 2665, 2678, 2906, 2995, 3023
Lsp1109I GCAGC 8 cut(s) 406, 1011, 1297, 1524, 1720, 1774, 1931, 2951
MaeI CTAG 5 cut(s) 1215, 1245, 1326, 1814, 2042
MaeII ACGT 8 cut(s) 191, 309, 603, 671, 683, 1501, 2391, 2780
MaeIII GTNAC 4 cut(s) 866, 1415, 1699, 2144
MbiI CCGCTC 1 cut(s) 180
MflI RGATCY 5 cut(s) 1957, 2096, 2226, 2803, 3037
MhlI GDGCHC 5 cut(s) 581, 2205, 2713, 2897, 2956
MluCI AATT 9 cut(s) 886, 916, 965, 1618, 1717, 1942, 2629, 2810, 2916
MlyI GAGTC 6 cut(s) 83, 483, 500, 532, 565, 1401
MmeI TCCRAC 4 cut(s) 423, 576, 1590, 2620
MroI TCCGGA 3 cut(s) 483, 1651, 2662
MroNI GCCGGC 1 cut(s) 1329
MseI TTAA 7 cut(s) 747, 1842, 2367, 2522, 2808, 2852, 2919
MslI CAYNNNNRTG 2 cut(s) 974, 2691
MspA1I CMGCKG 2 cut(s) 96, 340
MspCI CTTAAG 2 cut(s) 1841, 2366
MspR9I CCNGG 7 cut(s) 227, 460, 739, 757, 2318, 2319, 2817
Mva1269I GAATGC 1 cut(s) 981
MvaI CCWGG 1 cut(s) 2817
MvnI CGCG 5 cut(s) 96, 410, 662, 1260, 1599
MwoI GCNNNNNNNGC 7 cut(s) 193, 416, 789, 1768, 1834, 2162, 2267
NaeI GCCGGC 1 cut(s) 1331
NciI CCSGG 6 cut(s) 227, 460, 739, 757, 2318, 2319
NgoMIV GCCGGC 1 cut(s) 1329
NlaIII CATG 8 cut(s) 294, 1345, 2161, 2185, 2294, 2492, 2608, 2971
NlaIV GGNNCC 9 cut(s) 60, 231, 499, 819, 904, 905, 1009, 1628, 2140
NmeAIII GCCGAG 1 cut(s) 448
NmuCI GTSAC 2 cut(s) 1415, 2144
NsbI TGCGCA 1 cut(s) 2215
NspI RCATGY 2 cut(s) 2294, 2492
NspV TTCGAA 3 cut(s) 644, 1724, 1976
PaeR7I CTCGAG 1 cut(s) 547
PceI AGGCCT 2 cut(s) 545, 2080
PciI ACATGT 1 cut(s) 2290
PcsI WCGNNNNNNNCGW 2 cut(s) 384, 566
PctI GAATGC 1 cut(s) 981
PdiI GCCGGC 1 cut(s) 1331
PfeI GAWTC 8 cut(s) 631, 721, 1046, 1054, 1142, 2038, 2375, 2943
PfoI TCCNGGA 1 cut(s) 2815
PinAI ACCGGT 2 cut(s) 161, 2141
Ple19I CGATCG 1 cut(s) 2061
PleI GAGTC 6 cut(s) 83, 482, 500, 531, 565, 1400
PpsI GAGTC 6 cut(s) 83, 482, 500, 531, 565, 1400
PpuMI RGGWCCY 2 cut(s) 903, 1637
PscI ACATGT 1 cut(s) 2290
PshAI GACNNNNGTC 1 cut(s) 686
PsiI TTATAA 1 cut(s) 891
Psp124BI GAGCTC 1 cut(s) 581
Psp5II RGGWCCY 2 cut(s) 903, 1637
Psp6I CCWGG 1 cut(s) 2815
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 1 cut(s) 2815
PspN4I GGNNCC 9 cut(s) 60, 231, 499, 819, 904, 905, 1009, 1628, 2140
PspPI GGNCC 7 cut(s) 298, 486, 497, 817, 903, 1008, 1637
PspPPI RGGWCCY 2 cut(s) 903, 1637
PsrI GAACNNNNNNTAC 2 cut(s) 851, 883
PstI CTGCAG 2 cut(s) 1764, 1903
PstNI CAGNNNCTG 3 cut(s) 789, 1100, 3032
PsuI RGATCY 5 cut(s) 1957, 2096, 2226, 2803, 3037
PvuI CGATCG 1 cut(s) 2061
RsaI GTAC 6 cut(s) 761, 814, 1110, 1242, 1592, 2654
RsaNI GTAC 6 cut(s) 760, 813, 1109, 1241, 1591, 2653
RseI CAYNNNNRTG 2 cut(s) 974, 2691
SacI GAGCTC 1 cut(s) 581
SacII CCGCGG 1 cut(s) 97
SalI GTCGAC 1 cut(s) 667
SaqAI TTAA 7 cut(s) 747, 1842, 2367, 2522, 2808, 2852, 2919
Sau96I GGNCC 7 cut(s) 298, 486, 497, 817, 903, 1008, 1637
SchI GAGTC 6 cut(s) 83, 483, 500, 532, 565, 1401
ScrFI CCNGG 7 cut(s) 227, 460, 739, 757, 2318, 2319, 2817
SduI GDGCHC 5 cut(s) 581, 2205, 2713, 2897, 2956
SfcI CTRYAG 3 cut(s) 1760, 1899, 3033
Sfr274I CTCGAG 1 cut(s) 547
Sfr303I CCGCGG 1 cut(s) 97
SfuI TTCGAA 3 cut(s) 644, 1724, 1976
SgrBI CCGCGG 1 cut(s) 97
SinI GGWCC 6 cut(s) 486, 497, 817, 903, 1008, 1637
SlaI CTCGAG 1 cut(s) 547
SmaI CCCGGG 1 cut(s) 2319
SmiMI CAYNNNNRTG 2 cut(s) 974, 2691
SmlI CTYRAG 4 cut(s) 302, 547, 1841, 2366
SmoI CTYRAG 4 cut(s) 302, 547, 1841, 2366
Sse9I AATT 9 cut(s) 886, 916, 965, 1618, 1717, 1942, 2629, 2810, 2916
SseBI AGGCCT 2 cut(s) 545, 2080
SspI AATATT 3 cut(s) 349, 1608, 2791
SspMI CTAG 5 cut(s) 1215, 1245, 1326, 1814, 2042
SstI GAGCTC 1 cut(s) 581
StuI AGGCCT 2 cut(s) 545, 2080
StyD4I CCNGG 7 cut(s) 225, 458, 737, 755, 2316, 2317, 2815
StyI CCWWGG 5 cut(s) 768, 1011, 1244, 1532, 2091
TaiI ACGT 8 cut(s) 194, 312, 606, 674, 686, 1504, 2394, 2783
TasI AATT 9 cut(s) 886, 916, 965, 1618, 1717, 1942, 2629, 2810, 2916
TatI WGTACW 2 cut(s) 1590, 2652
TauI GCSGC 5 cut(s) 99, 140, 410, 469, 1599
TfiI GAWTC 8 cut(s) 631, 721, 1046, 1054, 1142, 2038, 2375, 2943
Tru1I TTAA 7 cut(s) 747, 1842, 2367, 2522, 2808, 2852, 2919
Tru9I TTAA 7 cut(s) 747, 1842, 2367, 2522, 2808, 2852, 2919
TscAI CASTG 7 cut(s) 1122, 1828, 2053, 2418, 2432, 2698, 2703
TseFI GTSAC 2 cut(s) 1415, 2144
TseI GCWGC 8 cut(s) 419, 1024, 1285, 1537, 1708, 1762, 1919, 2964
Tsp45I GTSAC 2 cut(s) 1415, 2144
TspGWI ACGGA 3 cut(s) 461, 830, 2919
TspMI CCCGGG 1 cut(s) 2317
TspRI CASTG 7 cut(s) 1122, 1828, 2053, 2418, 2432, 2698, 2703
Vha464I CTTAAG 2 cut(s) 1841, 2366
VneI GTGCAC 1 cut(s) 2201
VpaK11BI GGWCC 6 cut(s) 486, 497, 817, 903, 1008, 1637
XagI CCTNNNNNAGG 1 cut(s) 550
XapI RAATTY 4 cut(s) 916, 965, 2629, 2810
XbaI TCTAGA 1 cut(s) 2041
XceI RCATGY 2 cut(s) 2294, 2492
XcmI CCANNNNNNNNNTGG 4 cut(s) 25, 1959, 2164, 2887
XhoI CTCGAG 1 cut(s) 547
XmaI CCCGGG 1 cut(s) 2317
XmaJI CCTAGG 1 cut(s) 1244
XmiI GTMKAC 1 cut(s) 668
XspI CTAG 5 cut(s) 1215, 1245, 1326, 1814, 2042
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.