Rw5G038050

PAS fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004648
Physical Location & Seq
Forward (+)
67246935 .. 67254808
7874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038050.1

Sequence Viewer

Length: 3090 bp
ATGCAGAGAAGAAGAAGATCGGAAATGGAAGACGAACATTCCGAAACGCCAGCCTCACTCATTCCCCCACTCCCAAGAGACTCCCGCGGCTCCCTCGAAGTCTTCAACCCCTCCTCCTCGACCCGACCCGCCAGCCCCCCACCCTTCCGTCCTTCCCAACCCGCCTCCTGGAACACCTGGATCGACCCACGTGACCCCAAACCCGACCCGCCACCCAACGCCGCCTCCAAGTCCGGCCGCGACGGCGACGGCACCACCACCTCATGGATGGCCCTCAAGGACTTCCCCCCAACTCCACCGCCGCCGTCCAAGCCGTCCAAGCAGTCCCAGCAATCCCCGCCGCTCATCCAGCAGATCATCTCCGCCTCGGTCAACGACAAGTCAACGCCGGAGCAGAGCGACGCGGCGCAGAGGGCCGCCGAGTGGGGATTGGTTCTAAAGACGGACACGGAGACGGGGAGGCCGCAGGGAGTGACCACCCGGAACTCGGGCGGACCCGAAGACCCGAGCCACAAACCGGGTACCTCCAGAAGAAACTCCAACAACTCGGTCCGGAGCTCCGGGGAGTTGTCCGACGACGGCGGCGGCGGAGGAGGAGGGAAAGGGTTTCCGAGAGCTTCGGAGGACTTGAAGAACGTGCTGTCGACGTTTCAGCAGACGTTCGTGGTGTCGGATGCTACCAAACCCGATTACCCGATCATGTACGCCAGCGCCGGGTTTTTCAAGATGACCGGGTACACCTCCAAGGAGGTCATCGGCAGGAACTGCCGGTTTTTGCAGGGAGCGGATACGGACCCGGACGACGTGGCGCAGCTGAGGGAGGCTTTGCAGAAGAACACGAGCTATTGCGGTCGGTTGTTGAATTATAAGAAAGATGGGACCCCTTTTTGGAATTTACTTACTATTGCTCCTATCAAGGACGAGACTGGGAAGGTCCTCAAATTCATTGGAATGCAAGTGGAGGTGAGCAAGCACACTGAGGGGTCCAAGGATAAGATGCTGCGCCCCAATGGATTGCCTGAATCTCTCATTCGATATGATGCTCGTCAGAAAGAGATGGCTACACATTCAGTAACTGAGCTTGTGCAGGCAGTGAGGAGACCGAGGTCACTTAGTGAATCGACAAATCGTCCCTTTAGAAAGTCCGGAGGTGGCGGTAGGGGAGAGGAGGTAATAGAAGCTCTTGCGAGGAGAAACTCCGAGAGTTTGCCTAGGAGAAACTCAGAGAATGTGGCACCACCTAGACGTAACTCACTTGGGGATGCTAAAATCTCAATGCAGTGTATCAATGAGGTTCCTGAAAAGAAACAGAAAAAATCTAGACGGCGCTCTTTCATGGGGGTTATTCGGAAAAGTCAAACTCAAAGCCAAACCGATGATGATACATTTGACAACTTTGGAGATTCTGAGAATGTCCGTGATGACAGTGACAATGATGAGGGACCTATGAGTCTTGAAGATAAAGCGAGGAAGAAGGAAATGAGGAAGGGTATGGATCTTGCTACGACGCTGGAACGTATAGAGAAAAACTTTGTCATTACTGATCCAAGGCTGCCTGATAATCCCATTATATTTGCATCCGACAGCTTCTTAGAGCTAACGGAGTACAGCCGCGAAGAAATATTGGGAAGAAATTGCAGATTCCTTCAAGGACCTGAGACTGATCCAGTAACTGTGAAAAAAATAAGAGAGGCAATTGACAACCAGACTGACGTGACTGTACAACTCATCAATTACACGAAAAGTGGCAAAAAGTTCTGGAATGTCTTTCATCTGCAGCCTATGCGTGATCAAAAGGGAGAAGTACAGTATTTTATTGGAGTACAACTTGATGGCAGTCAGCACATTGAGCCTCTGCATAACTCTATTCCTGAAGTTACAGCAAAAGAGAGTGAAAAACTGGTCAAAGAGACTGCAGTTAATGTTGATGAGGCAGCAAGAGAACTTCCAGATGCCAATATGAAACCAGAAGATTTATGGATGAATCATTCGAAGGTCGTTCATCCAAAGCCTCATAGAAGGGACAGCCCCCCTTGGAGAGCTATTCAAAAGATTCTAGACAGTGGAGAACAGATAGGATTGAAACATTTTAAGCCTATAAAGCCCTTGGGATCTGGTGATACTGGCAGTGTGCATTTGGTCCAATTGTGTGGAACTGATCAGTACTACGCCATGAAGGCTATGGATAAGGGCATCATGCTCAATAGGAACAAGGTGCACAGAGCTTGTGCGGAGAGAGAAATCCTCGACATGTTGGATCACCCTTTTCTTCCTGCATTGTATGCTTCGTTTCAGACCAAAACACATGTTTGCCTGATCACTGATTACTACCCTGGTGGAGAACTCTTCCTACTTTTGGACAGCCAACCAGCGAAGGTCCTGAAGGAAGATTCAGTGAGATTTTATGTTGCAGAGGTAGTTGTTGCCTTGGAGTATCTTCACTGTCAAGGGATAATTTACAGGGATTTGAAGCCTGAAAATGTTTTACTTCAGAGCAGTGGGCACGTAACCCTGACCGATTTTGATTTATCGTGTTTGACATCTTGCAAACCACAGCTTTTAATTCCTGCCATAAACGAAAAGAAAAGGCATCACAAAGGTCAACAGAATCCAATCTTCATGGCTGAACCAATGAGAGCATCAAATTCTTTTGTTGGCACGGAAGAGTACATCGCTCCGGAAATCATAACTGGAGCTGGCCATTCTAGTGCAGTAGACTGGTGGGCTCTTGGTATTCTTATATATGAAATGCTCTATGGATATACACCATTTAGGGGAAAGACAAGGCAAAAGACGTTCGCCAATATTCTTCACAAGGATCTCAAATTTCCAGGAAGTATACCGGCAAGTCTCCAAGCAAAACAGTTAATGTATCGGTTGTTGCATCGTGATCCCAAAAACAGATTGGGGTCTCAGGAAGGGGCAAGCGAAATTAAGCGTCACCCTTTCTTTCGGGGTGTCAACTGGGCTCTAGTTCGCTGCATGAACCCTCCTCAACTCGATGCTCCTCTCTTCGGGACAACTGAAGCTGAAAAGGGTGCTAAGCTTGTGGACCCTGAGATGCAAGATCTACAAACAAATATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

1029

Amino Acids

115.25

Weight (kDa)

8.46

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 227 - 323 3.4e-20 PAS domain
PAS PF00989 232 - 318 7.4e-08 PAS fold
PAS_3 PF08447 233 - 317 2.5e-07 PAS fold
PAS_9 PF13426 519 - 611 1e-19 PAS domain
PAS PF00989 522 - 611 1.6e-06 PAS fold
PAS_3 PF08447 523 - 608 6e-07 PAS fold
Pkinase PF00069 699 - 984 9e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 700 - 846 1.4e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 889 - 969 5.4e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
AasI GACNNNNNNGTC 1 cut(s) 1449
Acc65I GGTACC 1 cut(s) 521
AccB1I GGYRCC 3 cut(s) 251, 521, 1234
AccB7I CCANNNNNTGG 1 cut(s) 264
AccBSI CCGCTC 2 cut(s) 343, 785
AccI GTMKAC 3 cut(s) 644, 2715, 2839
AccII CGCG 4 cut(s) 87, 240, 404, 1614
AccIII TCCGGA 3 cut(s) 552, 1145, 2677
AclWI GGATC 8 cut(s) 188, 1503, 1538, 1658, 2119, 2265, 2826, 2885
AcoI YGGCCR 2 cut(s) 235, 2698
AcsI RAATTY 4 cut(s) 892, 941, 2644, 2825
AcuI CTGAAG 4 cut(s) 1893, 2402, 2474, 3045
AcvI CACGTG 1 cut(s) 191
AdeI CACNNNGTG 1 cut(s) 1115
AfaI GTAC 9 cut(s) 523, 704, 737, 1607, 1722, 1806, 1824, 2165, 2669
AflIII ACRYGT 2 cut(s) 2250, 2305
AgsI TTSAA 9 cut(s) 106, 631, 724, 862, 1457, 1649, 2048, 2083, 2470
AhdI GACNNNNNGTC 1 cut(s) 1128
AjiI CACGTC 2 cut(s) 805, 1714
AjnI CCWGG 4 cut(s) 167, 176, 2332, 2830
Alw21I GWGCWC 2 cut(s) 560, 2220
Alw26I GTCTC 8 cut(s) 72, 446, 917, 1093, 1652, 1904, 2855, 2916
Alw44I GTGCAC 1 cut(s) 2216
AlwI GGATC 8 cut(s) 188, 1503, 1538, 1658, 2119, 2265, 2826, 2885
AlwNI CAGNNNCTG 3 cut(s) 765, 1076, 1673
Ama87I CYCGRG 2 cut(s) 487, 505
Aor13HI TCCGGA 3 cut(s) 552, 1145, 2677
AoxI GGCC 5 cut(s) 235, 270, 414, 461, 2698
ApaLI GTGCAC 1 cut(s) 2216
ApeKI GCWGC 6 cut(s) 811, 1000, 1552, 1777, 1934, 2979
ApoI RAATTY 4 cut(s) 892, 941, 2644, 2825
Asp718I GGTACC 1 cut(s) 521
AspA2I CCTAGG 1 cut(s) 1211
AspLEI GCGC 5 cut(s) 409, 713, 811, 1005, 1329
AsuC2I CCSGG 6 cut(s) 481, 519, 562, 715, 733, 797
AsuHPI GGTGA 4 cut(s) 976, 2129, 2252, 2933
AsuII TTCGAA 1 cut(s) 1991
AvaI CYCGRG 2 cut(s) 487, 505
AvrII CCTAGG 1 cut(s) 1211
BaeGI GKGCMC 2 cut(s) 2220, 2505
BalI TGGCCA 1 cut(s) 2700
BanI GGYRCC 3 cut(s) 251, 521, 1234
BanII GRGCYC 3 cut(s) 560, 2728, 2971
BauI CACGAG 1 cut(s) 838
BbrPI CACGTG 1 cut(s) 191
BbsI GAAGAC 3 cut(s) 36, 94, 507
Bbv12I GWGCWC 2 cut(s) 560, 2220
BbvCI CCTCAGC 1 cut(s) 815
BbvI GCAGC 6 cut(s) 823, 987, 1539, 1789, 1946, 2966
BccI CCATC 4 cut(s) 262, 869, 1051, 1826
BceAI ACGGC 6 cut(s) 259, 265, 289, 298, 595, 1340
BciT130I CCWGG 4 cut(s) 169, 178, 2334, 2832
BciVI GTATCC 1 cut(s) 781
BclI TGATCA 3 cut(s) 1789, 2158, 2316
BcnI CCSGG 6 cut(s) 481, 519, 562, 715, 733, 797
BcoDI GTCTC 8 cut(s) 72, 446, 917, 1093, 1652, 1904, 2855, 2916
BfaI CTAG 6 cut(s) 1212, 1242, 1320, 2057, 2706, 2972
BfmI CTRYAG 2 cut(s) 1775, 1914
BfoI RGCGCY 2 cut(s) 714, 1330
BfuI GTATCC 1 cut(s) 781
BglI GCCNNNNNGGC 2 cut(s) 243, 2177
BglII AGATCT 1 cut(s) 3067
BlnI CCTAGG 1 cut(s) 1211
BlpI GCTNAGC 1 cut(s) 3042
BmcAI AGTACT 1 cut(s) 2165
BmeRI GACNNNNNGTC 1 cut(s) 1128
BmeT110I CYCGRG 2 cut(s) 487, 505
BmgBI CACGTC 2 cut(s) 805, 1714
BmrI ACTGGG 2 cut(s) 936, 2974
BmuI ACTGGG 2 cut(s) 936, 2974
BoxI GACNNNNGTC 1 cut(s) 1105
BpiI GAAGAC 3 cut(s) 36, 94, 507
BplI GAGNNNNNCTC 2 cut(s) 2229, 2261
BpmI CTGGAG 2 cut(s) 511, 2712
Bpu10I CCTNAGC 1 cut(s) 815
Bpu1102I GCTNAGC 1 cut(s) 3042
Bpu14I TTCGAA 1 cut(s) 1991
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 6 cut(s) 481, 519, 562, 715, 733, 797
BsaAI YACGTR 2 cut(s) 191, 2506
BsaI GGTCTC 2 cut(s) 1093, 2916
BsaWI WCCGGW 3 cut(s) 552, 1145, 2677
BsaXI ACNNNNNCTCC 8 cut(s) 98, 128, 209, 239, 892, 922, 1155, 1185
Bse118I RCCGGY 2 cut(s) 768, 2842
Bse1I ACTGG 7 cut(s) 931, 1667, 1905, 2128, 2695, 2723, 2969
BseAI TCCGGA 3 cut(s) 552, 1145, 2677
BseBI CCWGG 4 cut(s) 169, 178, 2334, 2832
BseGI GGATG 7 cut(s) 273, 345, 679, 1267, 1577, 1986, 2002
BseMII CTCAG 8 cut(s) 806, 969, 1068, 1236, 1398, 1647, 2927, 3048
BseNI ACTGG 7 cut(s) 931, 1667, 1905, 2128, 2695, 2723, 2969
BseRI GAGGAG 9 cut(s) 103, 106, 606, 609, 1111, 1181, 1204, 2982, 2997
BseSI GKGCMC 2 cut(s) 2220, 2505
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 6 cut(s) 823, 987, 1539, 1789, 1946, 2966
BseYI CCCAGC 1 cut(s) 327
BsgI GTGCAG 2 cut(s) 1106, 2730
Bsh1236I CGCG 4 cut(s) 87, 240, 404, 1614
Bsh1285I CGRYCG 2 cut(s) 238, 853
BshFI GGCC 5 cut(s) 237, 272, 416, 463, 2700
BshNI GGYRCC 3 cut(s) 251, 521, 1234
BsiEI CGRYCG 2 cut(s) 238, 853
BsiHKAI GWGCWC 2 cut(s) 560, 2220
BsiHKCI CYCGRG 2 cut(s) 487, 505
BslFI GGGAC 6 cut(s) 310, 892, 1116, 1455, 2036, 3031
BsmAI GTCTC 8 cut(s) 72, 446, 917, 1093, 1652, 1904, 2855, 2916
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 6 cut(s) 310, 892, 1116, 1455, 2036, 3031
BsmI GAATGC 1 cut(s) 957
BsnI GGCC 5 cut(s) 237, 272, 416, 463, 2700
Bso31I GGTCTC 2 cut(s) 1093, 2916
BsoBI CYCGRG 2 cut(s) 487, 505
Bsp119I TTCGAA 1 cut(s) 1991
Bsp1286I GDGCHC 5 cut(s) 560, 2220, 2505, 2728, 2971
Bsp13I TCCGGA 3 cut(s) 552, 1145, 2677
Bsp1407I TGTACA 1 cut(s) 1720
Bsp1720I GCTNAGC 1 cut(s) 3042
BspANI GGCC 5 cut(s) 237, 272, 416, 463, 2700
BspCNI CTCAG 8 cut(s) 807, 970, 1069, 1235, 1399, 1648, 2926, 3049
BspEI TCCGGA 3 cut(s) 552, 1145, 2677
BspFNI CGCG 4 cut(s) 87, 240, 404, 1614
BspMAI CTGCAG 2 cut(s) 1779, 1918
BspPI GGATC 8 cut(s) 188, 1503, 1538, 1658, 2119, 2265, 2826, 2885
BspT104I TTCGAA 1 cut(s) 1991
BspT107I GGYRCC 3 cut(s) 251, 521, 1234
BspTNI GGTCTC 2 cut(s) 1093, 2916
BsrBI CCGCTC 2 cut(s) 343, 785
BsrFI RCCGGY 2 cut(s) 768, 2842
BsrGI TGTACA 1 cut(s) 1720
BsrI ACTGG 7 cut(s) 931, 1667, 1905, 2128, 2695, 2723, 2969
BssAI RCCGGY 2 cut(s) 768, 2842
BssNAI GTATAC 1 cut(s) 2840
BssSI CACGAG 1 cut(s) 838
BssT1I CCWWGG 7 cut(s) 744, 987, 1211, 1547, 2033, 2106, 2427
Bst1107I GTATAC 1 cut(s) 2840
Bst2BI CACGAG 1 cut(s) 838
Bst2UI CCWGG 4 cut(s) 169, 178, 2334, 2832
Bst4CI ACNGT 7 cut(s) 1427, 1675, 1720, 1809, 2063, 2444, 2865
Bst6I CTCTTC 3 cut(s) 2351, 2658, 3017
BstAPI GCANNNNNTGC 3 cut(s) 765, 1783, 2282
BstAUI TGTACA 1 cut(s) 1720
BstBAI YACGTR 2 cut(s) 191, 2506
BstBI TTCGAA 1 cut(s) 1991
BstC8I GCNNGC 7 cut(s) 51, 133, 709, 971, 1089, 2698, 2926
BstDSI CCRYGG 1 cut(s) 85
BstF5I GGATG 7 cut(s) 273, 345, 679, 1267, 1577, 1986, 2002
BstFNI CGCG 4 cut(s) 87, 240, 404, 1614
BstH2I RGCGCY 2 cut(s) 714, 1330
BstHHI GCGC 5 cut(s) 409, 713, 811, 1005, 1329
BstMAI GTCTC 8 cut(s) 72, 446, 917, 1093, 1652, 1904, 2855, 2916
BstMCI CGRYCG 2 cut(s) 238, 853
BstNI CCWGG 4 cut(s) 169, 178, 2334, 2832
BstNSI RCATGY 2 cut(s) 2254, 2309
BstPAI GACNNNNGTC 1 cut(s) 1105
BstSFI CTRYAG 2 cut(s) 1775, 1914
BstSLI GKGCMC 2 cut(s) 2220, 2505
BstUI CGCG 4 cut(s) 87, 240, 404, 1614
BstV1I GCAGC 6 cut(s) 823, 987, 1539, 1789, 1946, 2966
BstV2I GAAGAC 3 cut(s) 36, 94, 507
BstX2I RGATCY 4 cut(s) 1495, 2111, 2818, 3067
BstXI CCANNNNNNTGG 1 cut(s) 2150
BstYI RGATCY 4 cut(s) 1495, 2111, 2818, 3067
BstZ17I GTATAC 1 cut(s) 2840
BstZI CGGCCG 1 cut(s) 235
BsuI GTATCC 1 cut(s) 781
BsuRI GGCC 5 cut(s) 237, 272, 416, 463, 2700
BtgI CCRYGG 1 cut(s) 85
BtgZI GCGATG 1 cut(s) 2656
BtrI CACGTC 2 cut(s) 805, 1714
BtsCI GGATG 7 cut(s) 273, 345, 679, 1267, 1577, 1986, 2002
BtsI GCAGTG 4 cut(s) 1098, 1286, 2134, 2503
Cac8I GCNNGC 7 cut(s) 51, 133, 709, 971, 1089, 2698, 2926
CaiI CAGNNNCTG 3 cut(s) 765, 1076, 1673
CfoI GCGC 5 cut(s) 409, 713, 811, 1005, 1329
Cfr10I RCCGGY 2 cut(s) 768, 2842
Cfr42I CCGCGG 1 cut(s) 88
CpoI CGGWCCG 1 cut(s) 550
CseI GACGC 3 cut(s) 410, 1516, 2927
Csp6I GTAC 9 cut(s) 522, 703, 736, 1606, 1721, 1805, 1823, 2164, 2668
CspI CGGWCCG 1 cut(s) 550
CviAII CATG 9 cut(s) 264, 700, 1336, 2173, 2197, 2251, 2306, 2620, 2983
CviQI GTAC 9 cut(s) 522, 703, 736, 1606, 1721, 1805, 1823, 2164, 2668
DraIII CACNNNGTG 1 cut(s) 1115
DrdI GACNNNNNNGTC 1 cut(s) 1449
DriI GACNNNNNGTC 1 cut(s) 1128
DseDI GACNNNNNNGTC 1 cut(s) 1449
EaeI YGGCCR 2 cut(s) 235, 2698
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 2351, 2658, 3017
Eam1105I GACNNNNNGTC 1 cut(s) 1128
EarI CTCTTC 3 cut(s) 2351, 2658, 3017
EciI GGCGGA 3 cut(s) 352, 507, 603
Ecl136II GAGCTC 1 cut(s) 558
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 7 cut(s) 744, 987, 1211, 1547, 2033, 2106, 2427
Eco24I GRGCYC 3 cut(s) 560, 2728, 2971
Eco31I GGTCTC 2 cut(s) 1093, 2916
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 558
Eco57I CTGAAG 4 cut(s) 1893, 2402, 2474, 3045
Eco72I CACGTG 1 cut(s) 191
Eco88I CYCGRG 2 cut(s) 487, 505
EcoICRI GAGCTC 1 cut(s) 558
EcoO109I RGGNCCY 5 cut(s) 879, 934, 1442, 1652, 2377
EcoRII CCWGG 4 cut(s) 167, 176, 2332, 2830
EcoT14I CCWWGG 7 cut(s) 744, 987, 1211, 1547, 2033, 2106, 2427
EcoT38I GRGCYC 3 cut(s) 560, 2728, 2971
ErhI CCWWGG 7 cut(s) 744, 987, 1211, 1547, 2033, 2106, 2427
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 9 cut(s) 267, 703, 1339, 2176, 2200, 2254, 2309, 2623, 2986
FaqI GGGAC 6 cut(s) 310, 892, 1116, 1455, 2036, 3031
FatI CATG 9 cut(s) 263, 699, 1335, 2172, 2196, 2250, 2305, 2619, 2982
FauI CCCGC 5 cut(s) 92, 136, 169, 216, 345
FbaI TGATCA 3 cut(s) 1789, 2158, 2316
FblI GTMKAC 3 cut(s) 644, 2715, 2839
FokI GGATG 7 cut(s) 280, 332, 686, 1274, 1564, 1989, 1993
FriOI GRGCYC 3 cut(s) 560, 2728, 2971
FspBI CTAG 6 cut(s) 1212, 1242, 1320, 2057, 2706, 2972
GlaI GCGC 5 cut(s) 408, 712, 810, 1004, 1328
GsaI CCCAGC 1 cut(s) 331
GsuI CTGGAG 2 cut(s) 511, 2712
HaeII RGCGCY 2 cut(s) 714, 1330
HaeIII GGCC 5 cut(s) 237, 272, 416, 463, 2700
HgaI GACGC 3 cut(s) 410, 1516, 2927
HhaI GCGC 5 cut(s) 409, 713, 811, 1005, 1329
Hin1II CATG 9 cut(s) 267, 703, 1339, 2176, 2200, 2254, 2309, 2623, 2986
Hin6I GCGC 5 cut(s) 407, 711, 809, 1003, 1327
HinP1I GCGC 5 cut(s) 407, 711, 809, 1003, 1327
HincII GTYRAC 5 cut(s) 373, 384, 645, 2603, 2962
HindII GTYRAC 5 cut(s) 373, 384, 645, 2603, 2962
HindIII AAGCTT 1 cut(s) 3044
HphI GGTGA 4 cut(s) 976, 2129, 2252, 2933
Hpy99I CGWCG 8 cut(s) 245, 251, 404, 578, 581, 649, 806, 1510
HpyCH4III ACNGT 7 cut(s) 1427, 1675, 1720, 1809, 2063, 2444, 2865
Hsp92II CATG 9 cut(s) 267, 703, 1339, 2176, 2200, 2254, 2309, 2623, 2986
HspAI GCGC 5 cut(s) 407, 711, 809, 1003, 1327
KflI GGGWCCC 1 cut(s) 879
Kpn2I TCCGGA 3 cut(s) 552, 1145, 2677
KpnI GGTACC 1 cut(s) 525
Ksp22I TGATCA 3 cut(s) 1789, 2158, 2316
KspI CCGCGG 1 cut(s) 88
LmnI GCTCC 9 cut(s) 95, 391, 555, 563, 782, 913, 2680, 2693, 3010
Lsp1109I GCAGC 6 cut(s) 823, 987, 1539, 1789, 1946, 2966
MaeI CTAG 6 cut(s) 1212, 1242, 1320, 2057, 2706, 2972
MbiI CCGCTC 2 cut(s) 343, 785
MfeI CAATTG 2 cut(s) 1695, 2144
MflI RGATCY 4 cut(s) 1495, 2111, 2818, 3067
MhlI GDGCHC 5 cut(s) 560, 2220, 2505, 2728, 2971
MlsI TGGCCA 1 cut(s) 2700
MluNI TGGCCA 1 cut(s) 2700
MlyI GAGTC 2 cut(s) 74, 1459
MmeI TCCRAC 5 cut(s) 564, 597, 651, 1605, 2235
Mox20I TGGCCA 1 cut(s) 2700
MroI TCCGGA 3 cut(s) 552, 1145, 2677
MscI TGGCCA 1 cut(s) 2700
MseI TTAA 5 cut(s) 1920, 2091, 2561, 2867, 2934
MslI CAYNNNNRTG 2 cut(s) 950, 2706
Msp20I TGGCCA 1 cut(s) 2700
MspA1I CMGCKG 2 cut(s) 87, 814
MunI CAATTG 2 cut(s) 1695, 2144
Mva1269I GAATGC 1 cut(s) 957
MvaI CCWGG 4 cut(s) 169, 178, 2334, 2832
MvnI CGCG 4 cut(s) 87, 240, 404, 1614
NciI CCSGG 6 cut(s) 481, 519, 562, 715, 733, 797
NlaIII CATG 9 cut(s) 267, 703, 1339, 2176, 2200, 2254, 2309, 2623, 2986
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 6 cut(s) 191, 472, 1107, 1427, 1714, 2939
NspI RCATGY 2 cut(s) 2254, 2309
NspV TTCGAA 1 cut(s) 1991
PciI ACATGT 2 cut(s) 2250, 2305
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 957
PfeI GAWTC 8 cut(s) 1022, 1118, 1403, 1641, 1984, 2053, 2390, 2608
PflMI CCANNNNNTGG 1 cut(s) 264
PfoI TCCNGGA 2 cut(s) 167, 2830
PleI GAGTC 2 cut(s) 74, 1458
PmaCI CACGTG 1 cut(s) 191
PmlI CACGTG 1 cut(s) 191
PpsI GAGTC 2 cut(s) 74, 1458
Ppu21I YACGTR 2 cut(s) 191, 2506
PpuMI RGGWCCY 5 cut(s) 879, 934, 1442, 1652, 2377
PscI ACATGT 2 cut(s) 2250, 2305
PshAI GACNNNNGTC 1 cut(s) 1105
PsiI TTATAA 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 560
Psp5II RGGWCCY 5 cut(s) 879, 934, 1442, 1652, 2377
Psp6I CCWGG 4 cut(s) 167, 176, 2332, 2830
PspCI CACGTG 1 cut(s) 191
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 4 cut(s) 167, 176, 2332, 2830
PspPPI RGGWCCY 5 cut(s) 879, 934, 1442, 1652, 2377
PsrI GAACNNNNNNTAC 2 cut(s) 2334, 2366
PstI CTGCAG 2 cut(s) 1779, 1918
PstNI CAGNNNCTG 3 cut(s) 765, 1076, 1673
PsuI RGATCY 4 cut(s) 1495, 2111, 2818, 3067
PvuII CAGCTG 1 cut(s) 814
RsaI GTAC 9 cut(s) 523, 704, 737, 1607, 1722, 1806, 1824, 2165, 2669
RsaNI GTAC 9 cut(s) 522, 703, 736, 1606, 1721, 1805, 1823, 2164, 2668
RseI CAYNNNNRTG 2 cut(s) 950, 2706
Rsr2I CGGWCCG 1 cut(s) 550
RsrII CGGWCCG 1 cut(s) 550
SacI GAGCTC 1 cut(s) 560
SacII CCGCGG 1 cut(s) 88
SalI GTCGAC 1 cut(s) 643
SaqAI TTAA 5 cut(s) 1920, 2091, 2561, 2867, 2934
ScaI AGTACT 1 cut(s) 2165
SchI GAGTC 2 cut(s) 74, 1459
SduI GDGCHC 5 cut(s) 560, 2220, 2505, 2728, 2971
SfcI CTRYAG 2 cut(s) 1775, 1914
Sfr303I CCGCGG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 1991
SgrBI CCGCGG 1 cut(s) 88
SmiMI CAYNNNNRTG 2 cut(s) 950, 2706
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SspI AATATT 3 cut(s) 1623, 2806, 3082
SspMI CTAG 6 cut(s) 1212, 1242, 1320, 2057, 2706, 2972
SstI GAGCTC 1 cut(s) 560
StyI CCWWGG 7 cut(s) 744, 987, 1211, 1547, 2033, 2106, 2427
TaaI ACNGT 7 cut(s) 1427, 1675, 1720, 1809, 2063, 2444, 2865
TaqI TCGA 9 cut(s) 96, 119, 183, 644, 1033, 1121, 1991, 2247, 3000
TaqII GACCGA 4 cut(s) 358, 538, 1117, 2531
TatI WGTACW 6 cut(s) 1605, 1720, 1804, 1822, 2163, 2667
TfiI GAWTC 8 cut(s) 1022, 1118, 1403, 1641, 1984, 2053, 2390, 2608
Tru1I TTAA 5 cut(s) 1920, 2091, 2561, 2867, 2934
Tru9I TTAA 5 cut(s) 1920, 2091, 2561, 2867, 2934
TseFI GTSAC 6 cut(s) 191, 472, 1107, 1427, 1714, 2939
TseI GCWGC 6 cut(s) 811, 1000, 1552, 1777, 1934, 2979
Tsp45I GTSAC 6 cut(s) 191, 472, 1107, 1427, 1714, 2939
TspGWI ACGGA 7 cut(s) 137, 458, 464, 806, 1406, 1616, 2675
Van91I CCANNNNNTGG 1 cut(s) 264
VneI GTGCAC 1 cut(s) 2216
XapI RAATTY 4 cut(s) 892, 941, 2644, 2825
XbaI TCTAGA 2 cut(s) 1319, 2056
XceI RCATGY 2 cut(s) 2254, 2309
XcmI CCANNNNNNNNNTGG 4 cut(s) 265, 1974, 2179, 2902
XmaJI CCTAGG 1 cut(s) 1211
XmiI GTMKAC 3 cut(s) 644, 2715, 2839
XspI CTAG 6 cut(s) 1212, 1242, 1320, 2057, 2706, 2972
ZrmI AGTACT 1 cut(s) 2165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.