Rroxscaffold_1G00018900

PAS fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004648
Physical Location & Seq
Reverse (-)
23014210 .. 23024765
10556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00018900.1

Sequence Viewer

Length: 3060 bp
ATGGAAGACGAACATTCCGAAACGCCAGCCTCACTCATTCCCCCACTCCCAAGAGACTCCCGCGGCTCCCTCGAAGTCTTCAACCCCTCCACCCGACCCGCCAGCCCCCCACCACCCTTCCGACCTTCCCAACCCGCCTCCTGGAATACCTGGATCGACCCACGTGACACCCAACCCGACCCGCCACCCAACTCCGCCTCCAAGTCCGCCCTCGACGACGGCCCCATCACCTCATGGATGGCCCTCAAGGACTTCCCCTCAACTCCACCGCCGCCGTCCAAGCCTTCCATAAAGTCCCAGCAGTCCCCGCCGCTCATCCAGCAGACCATCTCCTCCTCGGTCAACGACAAGTCAACGCCGGAGCCGAGCGACGCCGCGCAGAGAGCCGCCGAGTGGGGATTGGTTCTGAAGACGGACACGGAGACGGGGAAGCCGCAGGGAGTGACCACCCGGAACTCGGGCGGGCCCGAAGACCCGAGCCACAAACCGGGTACTTCCAGAAGAAACTCCAACAACTCGGTCCGGAGCTCCGGGGAGTTGTCCGACGACGGCGGCGGCGGGGGAGGAGGAAAAGGGTTTCCGAGAGCTTCGGAGGACTTGAAGAACGTGCTGTCGACGTTTCAGCAGACGTTCGTGGTGTCGGATGCTACCAAACCCGATTACCCGATCATGTACGCCAGCGCCGGGTTTTTCAAGATGACCGGGTACACCTCCAAGGAGGTCATCGGCAGGAACTGCCGGTTTTTGCAGGGAGCGGATACGGACCCGGACGACGTGGCGCAGCTGAGGGAGGCTTTGCAAAAGAACACGAGCTATTGCGGTCGCTTGTTGAATTATAAGAAAGATGGGACCCCTTTTTGGAATTTACTTACTATTGCTCCTATCAAGGACGAAACTGGGAAGGTCCTCAAATTCATTGGAATGCAAGTGGAGGTGAGCAAGCACACTGAGGGGTCCAAGGATAAGATGCTGCGCCCCAATGGATTGCCTGAATCCCTCATTCGATATGACGCACGTCAGAAAGAGATGGCTACACATTCTGTAACTGAGCTTGTGCAGGCAGTGAGGAGACCGAGGTCACTTAGTGAATCGACAAATCGTCCCTTTAGAAAGTCCGGAGGTGGCGGTAGGGGAGAGGAGGTAATAGAAGCTCTTGCGAGGAGAAACTCCGAGAGTTTGCCTAGGAGAAACTCAGAGAATGTGGCACCACCTAGACGTAACTCACTTGGGGATGCTAAAATCTCAATGCAGTGTATCAATGAGGTTCCTGAAAAGAAACAGAAAAAATCTAGACGGCGCTCTTTCATGGGGGTTATTCGGAAAAGTCAAACTCAAAGCCAACCCGATGATGATACATTTGACAACTTTGGAGATTCTGAGGACGTCCGTGATGACAGTGACAATGATGAGAGACATATGAGTCTTGAAGATAAAGCGAGGAAGAAGGAAATGAGGAAGGGTATGGATCTTGCTACGACGCTGGAACGTATAGAGAAAAACTTTGTCATTACTGATCCAAGGCTGCCTGATAATCCCATTATATTTGCATCCGACAGCTTCTTAGAGCTAACGGAGTACAGCCGCGAAGAAATATTGGGAAGAAATTGCAGATTCCTTCAAGGACCTGAGACTGATCCAGCAACTGTGAAAAAAATAAGAGAGGCAATTGACAACCAGACTGACGTGACTGTACAACTCATCAATTACACGAAAAGCGGCAAAAAGTTCTGGAATGTCTTTCATCTGCAGCCTATGCGTGATCAAAAGGGAGAAGTACAGTATTTTATTGGAGTACAACTTGATGGCAGTCAGCACATTGAGCCTCTGCATAACTCTATTCCTGAAGTTACAGTAAAAGAGAGTGAAAAACTGATCAAAGAGACTGCAGTTAATGTTGATGAGGCAGCAAGAGAACTTCCAGATGCCAATATGAAACCAGAAGATTTATGGATGAATCATTCGAAGGTCGTTCATCCAAAGCCTCATAGAAGGGACAGCCCTCCTTGGAGAGCTATTCAGAAGATTCTAGACAGTGGAGAACAGATAGGATTGAAACATTTTAAGCCTATAAAGCCCTTGGGATCTGGTGATACTGGCAGTGTGCATTTGGTCCAATTGTGTGGAACTGATCAGTACTACGCCATGAAGGCTATGGATAAGGGTATCATGCTCAATAGGAACAAGGTGCACAGAGCTTGTGCGGAGAGAGAAATCCTCGACATGTTGGATCACCCTTTTCTTCCTGCATTGTATGCTTCGTTTCAGACCAAAACACATGTTTGCCTGATCACTGATTACTACCCTGGTGGAGAACTCTTCCTACTTTTGGACAGCCAACCAACGAAGGTCCTGAAGGAAGATTCAGTGAGATTTTATGTTGCAGAGGTAGTTGTTGCCTTGGAGTATCTTCACTGTCAAGGGATAATTTACAGGGATTTGAAGCCTGAAAATGTTTTACTTCAGAGCAGTGGGCACGTAACCCTGACCGATTTTGATTTATCGTGTTTGACATCTTGCAAACCACAGCTTTTAATTCCTACCATAAACCAAAAGAAAAGGCATCACAAAGGTCAACAGAATCCAATCTTCATGGCTGAACCAATGAGAGCATCAAATTCTTTTGTTGGCACGGAAGAGTACATCGCTCCGGAAATCATAACTGGAGCTGGCCATTCTAGTGCAGTAGACTGGTGGGCTCTTGGTATTCTTATATATGAAATGCTCTATGGATATACACCATTTAGGGGAAAGACAAGGCAAAAGACGTTCGCCAATATTCTTCACAAGGATCTCAAATTTCCAGGAAGTATACCGGCAAGTCTCCAAGCAAAACAGTTAATGTATCGGTTGTTGCATCGTGATCCCAAAAACAGATTGGGGTCACAGGAAGGGGCAAGCGAAATTAAGCGTCACCCTTTCTTTCGGGGTGTCAACTGGGCTCTAGTTCGCTGCATGAACCCTCCTCAACTCGATGCTCCTCTCTTCGGGACAACTGAAGCTGAAAAGGGTGCTAAGCTTGTGGATCCTGAGATGCAAGATCTACAAACAAATATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

1019

Amino Acids

114.14

Weight (kDa)

7.87

Isoelectric Point (pI)

49.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 217 - 313 3.3e-20 PAS domain
PAS PF00989 222 - 308 7.4e-08 PAS fold
PAS_3 PF08447 223 - 307 2.5e-07 PAS fold
PAS_9 PF13426 509 - 601 8.9e-20 PAS domain
PAS PF00989 512 - 601 4.4e-07 PAS fold
PAS_3 PF08447 513 - 598 3e-07 PAS fold
Pkinase PF00069 689 - 974 7.7e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 690 - 836 1.3e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 879 - 959 5.3e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 837
AasI GACNNNNNNGTC 1 cut(s) 1419
AatII GACGTC 1 cut(s) 1386
AccB1I GGYRCC 1 cut(s) 1204
AccBSI CCGCTC 2 cut(s) 313, 755
AccI GTMKAC 3 cut(s) 614, 2685, 2809
AccII CGCG 3 cut(s) 63, 377, 1584
AccIII TCCGGA 3 cut(s) 522, 1115, 2647
AcoI YGGCCR 1 cut(s) 2668
AcsI RAATTY 4 cut(s) 862, 911, 2614, 2795
AcuI CTGAAG 5 cut(s) 428, 1863, 2372, 2444, 3015
AcvI CACGTG 1 cut(s) 164
AcyI GRCGYC 2 cut(s) 372, 1383
AdeI CACNNNGTG 1 cut(s) 1085
AfaI GTAC 9 cut(s) 493, 674, 707, 1577, 1692, 1776, 1794, 2135, 2639
AfiI CCNNNNNNNGG 9 cut(s) 141, 393, 457, 487, 684, 858, 2326, 2744, 2984
AflIII ACRYGT 2 cut(s) 2220, 2275
AgsI TTSAA 8 cut(s) 82, 601, 694, 832, 1427, 1619, 2053, 2440
AhdI GACNNNNNGTC 1 cut(s) 1098
AjiI CACGTC 3 cut(s) 775, 1016, 1684
AjnI CCWGG 4 cut(s) 140, 149, 2302, 2800
Alw21I GWGCWC 2 cut(s) 530, 2190
Alw26I GTCTC 7 cut(s) 48, 416, 1063, 1405, 1622, 1874, 2825
Alw44I GTGCAC 1 cut(s) 2186
AlwNI CAGNNNCTG 2 cut(s) 735, 1643
Ama87I CYCGRG 2 cut(s) 457, 475
Aor13HI TCCGGA 3 cut(s) 522, 1115, 2647
AoxI GGCC 4 cut(s) 220, 240, 464, 2668
ApaI GGGCCC 1 cut(s) 468
ApaLI GTGCAC 1 cut(s) 2186
ApeKI GCWGC 6 cut(s) 781, 970, 1522, 1747, 1904, 2949
ApoI RAATTY 4 cut(s) 862, 911, 2614, 2795
AspA2I CCTAGG 1 cut(s) 1181
AspLEI GCGC 5 cut(s) 379, 683, 781, 975, 1299
AsuC2I CCSGG 6 cut(s) 451, 489, 532, 685, 703, 767
AsuHPI GGTGA 5 cut(s) 220, 946, 2099, 2222, 2903
AsuII TTCGAA 1 cut(s) 1961
AvaI CYCGRG 2 cut(s) 457, 475
AvaII GGWCC 8 cut(s) 520, 763, 849, 904, 954, 1622, 2110, 2347
AvrII CCTAGG 1 cut(s) 1181
BaeGI GKGCMC 3 cut(s) 468, 2190, 2475
BalI TGGCCA 1 cut(s) 2670
BamHI GGATCC 1 cut(s) 3022
BanI GGYRCC 1 cut(s) 1204
BanII GRGCYC 4 cut(s) 468, 530, 2698, 2941
BauI CACGAG 1 cut(s) 808
BbrPI CACGTG 1 cut(s) 164
BbsI GAAGAC 4 cut(s) 12, 70, 416, 477
Bbv12I GWGCWC 2 cut(s) 530, 2190
BbvCI CCTCAGC 1 cut(s) 785
BbvI GCAGC 6 cut(s) 793, 957, 1509, 1759, 1916, 2936
BccI CCATC 6 cut(s) 232, 233, 335, 839, 1021, 1796
BceAI ACGGC 4 cut(s) 235, 259, 565, 1310
BciT130I CCWGG 4 cut(s) 142, 151, 2304, 2802
BciVI GTATCC 1 cut(s) 751
BclI TGATCA 4 cut(s) 1759, 1872, 2128, 2286
BcnI CCSGG 6 cut(s) 451, 489, 532, 685, 703, 767
BcoDI GTCTC 7 cut(s) 48, 416, 1063, 1405, 1622, 1874, 2825
BfaI CTAG 6 cut(s) 1182, 1212, 1290, 2027, 2676, 2942
BfmI CTRYAG 2 cut(s) 1745, 1884
BfoI RGCGCY 2 cut(s) 684, 1300
BfuI GTATCC 1 cut(s) 751
BglI GCCNNNNNGGC 1 cut(s) 2147
BglII AGATCT 1 cut(s) 3037
BlnI CCTAGG 1 cut(s) 1181
BlpI GCTNAGC 1 cut(s) 3012
BmcAI AGTACT 1 cut(s) 2135
Bme18I GGWCC 8 cut(s) 520, 763, 849, 904, 954, 1622, 2110, 2347
BmeRI GACNNNNNGTC 1 cut(s) 1098
BmeT110I CYCGRG 2 cut(s) 457, 475
BmgBI CACGTC 3 cut(s) 775, 1016, 1684
BmrI ACTGGG 2 cut(s) 906, 2944
BmuI ACTGGG 2 cut(s) 906, 2944
BoxI GACNNNNGTC 2 cut(s) 1014, 1075
BpiI GAAGAC 4 cut(s) 12, 70, 416, 477
BplI GAGNNNNNCTC 2 cut(s) 2199, 2231
BpmI CTGGAG 1 cut(s) 2682
Bpu10I CCTNAGC 1 cut(s) 785
Bpu1102I GCTNAGC 1 cut(s) 3012
Bpu14I TTCGAA 1 cut(s) 1961
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 6 cut(s) 451, 489, 532, 685, 703, 767
BsaAI YACGTR 2 cut(s) 164, 2476
BsaHI GRCGYC 2 cut(s) 372, 1383
BsaI GGTCTC 1 cut(s) 1063
BsaWI WCCGGW 3 cut(s) 522, 1115, 2647
BsaXI ACNNNNNCTCC 6 cut(s) 182, 212, 862, 892, 1125, 1155
Bsc4I CCNNNNNNNGG 9 cut(s) 141, 393, 457, 487, 684, 858, 2326, 2744, 2984
Bse118I RCCGGY 2 cut(s) 738, 2812
Bse1I ACTGG 5 cut(s) 901, 2098, 2665, 2693, 2939
BseAI TCCGGA 3 cut(s) 522, 1115, 2647
BseBI CCWGG 4 cut(s) 142, 151, 2304, 2802
BseGI GGATG 7 cut(s) 243, 315, 649, 1237, 1547, 1956, 1972
BseLI CCNNNNNNNGG 9 cut(s) 141, 393, 457, 487, 684, 858, 2326, 2744, 2984
BseMII CTCAG 7 cut(s) 776, 939, 1038, 1206, 1368, 1617, 3018
BseNI ACTGG 5 cut(s) 901, 2098, 2665, 2693, 2939
BseRI GAGGAG 8 cut(s) 322, 325, 579, 1081, 1151, 1174, 2952, 2967
BseSI GKGCMC 3 cut(s) 468, 2190, 2475
BseXI GCAGC 6 cut(s) 793, 957, 1509, 1759, 1916, 2936
BseYI CCCAGC 1 cut(s) 297
BsgI GTGCAG 2 cut(s) 1076, 2700
Bsh1236I CGCG 3 cut(s) 63, 377, 1584
Bsh1285I CGRYCG 1 cut(s) 823
BshFI GGCC 4 cut(s) 222, 242, 466, 2670
BshNI GGYRCC 1 cut(s) 1204
BsiEI CGRYCG 1 cut(s) 823
BsiHKAI GWGCWC 2 cut(s) 530, 2190
BsiHKCI CYCGRG 2 cut(s) 457, 475
BslFI GGGAC 6 cut(s) 280, 289, 862, 1086, 2006, 3001
BslI CCNNNNNNNGG 9 cut(s) 141, 393, 457, 487, 684, 858, 2326, 2744, 2984
BsmAI GTCTC 7 cut(s) 48, 416, 1063, 1405, 1622, 1874, 2825
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 6 cut(s) 280, 289, 862, 1086, 2006, 3001
BsmI GAATGC 1 cut(s) 927
BsnI GGCC 4 cut(s) 222, 242, 466, 2670
Bso31I GGTCTC 1 cut(s) 1063
BsoBI CYCGRG 2 cut(s) 457, 475
Bsp119I TTCGAA 1 cut(s) 1961
Bsp120I GGGCCC 1 cut(s) 464
Bsp1286I GDGCHC 6 cut(s) 468, 530, 2190, 2475, 2698, 2941
Bsp13I TCCGGA 3 cut(s) 522, 1115, 2647
Bsp1407I TGTACA 1 cut(s) 1690
Bsp1720I GCTNAGC 1 cut(s) 3012
BspANI GGCC 4 cut(s) 222, 242, 466, 2670
BspCNI CTCAG 7 cut(s) 777, 940, 1039, 1205, 1369, 1618, 3019
BspEI TCCGGA 3 cut(s) 522, 1115, 2647
BspFNI CGCG 3 cut(s) 63, 377, 1584
BspMAI CTGCAG 2 cut(s) 1749, 1888
BspT104I TTCGAA 1 cut(s) 1961
BspT107I GGYRCC 1 cut(s) 1204
BspTNI GGTCTC 1 cut(s) 1063
BsrBI CCGCTC 2 cut(s) 313, 755
BsrFI RCCGGY 2 cut(s) 738, 2812
BsrGI TGTACA 1 cut(s) 1690
BsrI ACTGG 5 cut(s) 901, 2098, 2665, 2693, 2939
BssAI RCCGGY 2 cut(s) 738, 2812
BssNAI GTATAC 1 cut(s) 2810
BssNI GRCGYC 2 cut(s) 372, 1383
BssSI CACGAG 1 cut(s) 808
BssT1I CCWWGG 7 cut(s) 714, 957, 1181, 1517, 2003, 2076, 2397
Bst1107I GTATAC 1 cut(s) 2810
Bst2BI CACGAG 1 cut(s) 808
Bst2UI CCWGG 4 cut(s) 142, 151, 2304, 2802
Bst4CI ACNGT 8 cut(s) 1397, 1645, 1690, 1779, 1852, 2033, 2414, 2835
Bst6I CTCTTC 3 cut(s) 2321, 2628, 2987
BstACI GRCGYC 2 cut(s) 372, 1383
BstAPI GCANNNNNTGC 3 cut(s) 735, 1753, 2252
BstAUI TGTACA 1 cut(s) 1690
BstBAI YACGTR 2 cut(s) 164, 2476
BstBI TTCGAA 1 cut(s) 1961
BstC8I GCNNGC 8 cut(s) 27, 103, 464, 679, 941, 1059, 2668, 2896
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 7 cut(s) 243, 315, 649, 1237, 1547, 1956, 1972
BstFNI CGCG 3 cut(s) 63, 377, 1584
BstH2I RGCGCY 2 cut(s) 684, 1300
BstHHI GCGC 5 cut(s) 379, 683, 781, 975, 1299
BstMAI GTCTC 7 cut(s) 48, 416, 1063, 1405, 1622, 1874, 2825
BstMCI CGRYCG 1 cut(s) 823
BstNI CCWGG 4 cut(s) 142, 151, 2304, 2802
BstNSI RCATGY 2 cut(s) 2224, 2279
BstPAI GACNNNNGTC 2 cut(s) 1014, 1075
BstSFI CTRYAG 2 cut(s) 1745, 1884
BstSLI GKGCMC 3 cut(s) 468, 2190, 2475
BstUI CGCG 3 cut(s) 63, 377, 1584
BstV1I GCAGC 6 cut(s) 793, 957, 1509, 1759, 1916, 2936
BstV2I GAAGAC 4 cut(s) 12, 70, 416, 477
BstX2I RGATCY 5 cut(s) 1465, 2081, 2788, 3022, 3037
BstXI CCANNNNNNTGG 1 cut(s) 2120
BstYI RGATCY 5 cut(s) 1465, 2081, 2788, 3022, 3037
BstZ17I GTATAC 1 cut(s) 2810
BsuI GTATCC 1 cut(s) 751
BsuRI GGCC 4 cut(s) 222, 242, 466, 2670
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 2626
BtrI CACGTC 3 cut(s) 775, 1016, 1684
BtsCI GGATG 7 cut(s) 243, 315, 649, 1237, 1547, 1956, 1972
BtsI GCAGTG 4 cut(s) 1068, 1256, 2104, 2473
Cac8I GCNNGC 8 cut(s) 27, 103, 464, 679, 941, 1059, 2668, 2896
CaiI CAGNNNCTG 2 cut(s) 735, 1643
CfoI GCGC 5 cut(s) 379, 683, 781, 975, 1299
Cfr10I RCCGGY 2 cut(s) 738, 2812
Cfr42I CCGCGG 1 cut(s) 64
CpoI CGGWCCG 1 cut(s) 520
CseI GACGC 4 cut(s) 380, 1019, 1486, 2897
Csp6I GTAC 9 cut(s) 492, 673, 706, 1576, 1691, 1775, 1793, 2134, 2638
CspI CGGWCCG 1 cut(s) 520
CviAII CATG 9 cut(s) 234, 670, 1306, 2143, 2167, 2221, 2276, 2590, 2953
CviQI GTAC 9 cut(s) 492, 673, 706, 1576, 1691, 1775, 1793, 2134, 2638
DraIII CACNNNGTG 1 cut(s) 1085
DrdI GACNNNNNNGTC 1 cut(s) 1419
DriI GACNNNNNGTC 1 cut(s) 1098
DseDI GACNNNNNNGTC 1 cut(s) 1419
EaeI YGGCCR 1 cut(s) 2668
Eam1104I CTCTTC 3 cut(s) 2321, 2628, 2987
Eam1105I GACNNNNNGTC 1 cut(s) 1098
EarI CTCTTC 3 cut(s) 2321, 2628, 2987
EciI GGCGGA 2 cut(s) 184, 196
Ecl136II GAGCTC 1 cut(s) 528
Eco130I CCWWGG 7 cut(s) 714, 957, 1181, 1517, 2003, 2076, 2397
Eco24I GRGCYC 4 cut(s) 468, 530, 2698, 2941
Eco31I GGTCTC 1 cut(s) 1063
Eco47I GGWCC 8 cut(s) 520, 763, 849, 904, 954, 1622, 2110, 2347
Eco53kI GAGCTC 1 cut(s) 528
Eco57I CTGAAG 5 cut(s) 428, 1863, 2372, 2444, 3015
Eco72I CACGTG 1 cut(s) 164
Eco88I CYCGRG 2 cut(s) 457, 475
EcoICRI GAGCTC 1 cut(s) 528
EcoO109I RGGNCCY 4 cut(s) 849, 904, 1622, 2347
EcoRII CCWGG 4 cut(s) 140, 149, 2302, 2800
EcoT14I CCWWGG 7 cut(s) 714, 957, 1181, 1517, 2003, 2076, 2397
EcoT38I GRGCYC 4 cut(s) 468, 530, 2698, 2941
ErhI CCWWGG 7 cut(s) 714, 957, 1181, 1517, 2003, 2076, 2397
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 9 cut(s) 237, 673, 1309, 2146, 2170, 2224, 2279, 2593, 2956
FaqI GGGAC 6 cut(s) 280, 289, 862, 1086, 2006, 3001
FatI CATG 9 cut(s) 233, 669, 1305, 2142, 2166, 2220, 2275, 2589, 2952
FauI CCCGC 7 cut(s) 68, 106, 142, 189, 315, 455, 551
FauNDI CATATG 1 cut(s) 1416
FbaI TGATCA 4 cut(s) 1759, 1872, 2128, 2286
FblI GTMKAC 3 cut(s) 614, 2685, 2809
FokI GGATG 7 cut(s) 250, 302, 656, 1244, 1534, 1959, 1963
FriOI GRGCYC 4 cut(s) 468, 530, 2698, 2941
FspBI CTAG 6 cut(s) 1182, 1212, 1290, 2027, 2676, 2942
GlaI GCGC 5 cut(s) 378, 682, 780, 974, 1298
GsaI CCCAGC 1 cut(s) 301
GsuI CTGGAG 1 cut(s) 2682
HaeII RGCGCY 2 cut(s) 684, 1300
HaeIII GGCC 4 cut(s) 222, 242, 466, 2670
HgaI GACGC 4 cut(s) 380, 1019, 1486, 2897
HhaI GCGC 5 cut(s) 379, 683, 781, 975, 1299
Hin1I GRCGYC 2 cut(s) 372, 1383
Hin1II CATG 9 cut(s) 237, 673, 1309, 2146, 2170, 2224, 2279, 2593, 2956
Hin6I GCGC 5 cut(s) 377, 681, 779, 973, 1297
HinP1I GCGC 5 cut(s) 377, 681, 779, 973, 1297
HincII GTYRAC 5 cut(s) 343, 354, 615, 2573, 2932
HindII GTYRAC 5 cut(s) 343, 354, 615, 2573, 2932
HindIII AAGCTT 1 cut(s) 3014
HphI GGTGA 5 cut(s) 220, 946, 2099, 2222, 2903
Hpy166II GTNNAC 9 cut(s) 343, 354, 615, 708, 2188, 2573, 2686, 2810, 2932
Hpy8I GTNNAC 9 cut(s) 343, 354, 615, 708, 2188, 2573, 2686, 2810, 2932
Hpy99I CGWCG 8 cut(s) 218, 221, 374, 548, 551, 619, 776, 1480
HpyCH4III ACNGT 8 cut(s) 1397, 1645, 1690, 1779, 1852, 2033, 2414, 2835
Hsp92I GRCGYC 2 cut(s) 372, 1383
Hsp92II CATG 9 cut(s) 237, 673, 1309, 2146, 2170, 2224, 2279, 2593, 2956
HspAI GCGC 5 cut(s) 377, 681, 779, 973, 1297
KflI GGGWCCC 1 cut(s) 849
Kpn2I TCCGGA 3 cut(s) 522, 1115, 2647
Ksp22I TGATCA 4 cut(s) 1759, 1872, 2128, 2286
KspI CCGCGG 1 cut(s) 64
LmnI GCTCC 9 cut(s) 71, 361, 525, 533, 752, 883, 2650, 2663, 2980
Lsp1109I GCAGC 6 cut(s) 793, 957, 1509, 1759, 1916, 2936
MaeI CTAG 6 cut(s) 1182, 1212, 1290, 2027, 2676, 2942
MbiI CCGCTC 2 cut(s) 313, 755
MfeI CAATTG 2 cut(s) 1665, 2114
MflI RGATCY 5 cut(s) 1465, 2081, 2788, 3022, 3037
MhlI GDGCHC 6 cut(s) 468, 530, 2190, 2475, 2698, 2941
MlsI TGGCCA 1 cut(s) 2670
MluNI TGGCCA 1 cut(s) 2670
MlyI GAGTC 2 cut(s) 50, 1429
MmeI TCCRAC 6 cut(s) 145, 534, 567, 621, 1575, 2205
Mox20I TGGCCA 1 cut(s) 2670
MroI TCCGGA 3 cut(s) 522, 1115, 2647
MscI TGGCCA 1 cut(s) 2670
MseI TTAA 5 cut(s) 1890, 2061, 2531, 2837, 2904
MslI CAYNNNNRTG 2 cut(s) 920, 2676
Msp20I TGGCCA 1 cut(s) 2670
MspA1I CMGCKG 2 cut(s) 63, 784
MunI CAATTG 2 cut(s) 1665, 2114
Mva1269I GAATGC 1 cut(s) 927
MvaI CCWGG 4 cut(s) 142, 151, 2304, 2802
MvnI CGCG 3 cut(s) 63, 377, 1584
NciI CCSGG 6 cut(s) 451, 489, 532, 685, 703, 767
NdeI CATATG 1 cut(s) 1416
NlaIII CATG 9 cut(s) 237, 673, 1309, 2146, 2170, 2224, 2279, 2593, 2956
NmeAIII GCCGAG 2 cut(s) 390, 415
NmuCI GTSAC 7 cut(s) 164, 442, 1077, 1397, 1684, 2880, 2909
NspI RCATGY 2 cut(s) 2224, 2279
NspV TTCGAA 1 cut(s) 1961
PciI ACATGT 2 cut(s) 2220, 2275
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 362
PctI GAATGC 1 cut(s) 927
PfeI GAWTC 8 cut(s) 992, 1088, 1373, 1611, 1954, 2023, 2360, 2578
PfoI TCCNGGA 2 cut(s) 140, 2800
PleI GAGTC 2 cut(s) 50, 1428
PmaCI CACGTG 1 cut(s) 164
PmlI CACGTG 1 cut(s) 164
PpsI GAGTC 2 cut(s) 50, 1428
Ppu21I YACGTR 2 cut(s) 164, 2476
PpuMI RGGWCCY 4 cut(s) 849, 904, 1622, 2347
PscI ACATGT 2 cut(s) 2220, 2275
PshAI GACNNNNGTC 2 cut(s) 1014, 1075
PsiI TTATAA 1 cut(s) 837
Psp124BI GAGCTC 1 cut(s) 530
Psp5II RGGWCCY 4 cut(s) 849, 904, 1622, 2347
Psp6I CCWGG 4 cut(s) 140, 149, 2302, 2800
PspCI CACGTG 1 cut(s) 164
PspFI CCCAGC 1 cut(s) 297
PspGI CCWGG 4 cut(s) 140, 149, 2302, 2800
PspOMI GGGCCC 1 cut(s) 464
PspPPI RGGWCCY 4 cut(s) 849, 904, 1622, 2347
PsrI GAACNNNNNNTAC 2 cut(s) 2304, 2336
PstI CTGCAG 2 cut(s) 1749, 1888
PstNI CAGNNNCTG 2 cut(s) 735, 1643
PsuI RGATCY 5 cut(s) 1465, 2081, 2788, 3022, 3037
PvuII CAGCTG 1 cut(s) 784
RsaI GTAC 9 cut(s) 493, 674, 707, 1577, 1692, 1776, 1794, 2135, 2639
RsaNI GTAC 9 cut(s) 492, 673, 706, 1576, 1691, 1775, 1793, 2134, 2638
RseI CAYNNNNRTG 2 cut(s) 920, 2676
Rsr2I CGGWCCG 1 cut(s) 520
RsrII CGGWCCG 1 cut(s) 520
SacI GAGCTC 1 cut(s) 530
SacII CCGCGG 1 cut(s) 64
SalI GTCGAC 1 cut(s) 613
SaqAI TTAA 5 cut(s) 1890, 2061, 2531, 2837, 2904
ScaI AGTACT 1 cut(s) 2135
SchI GAGTC 2 cut(s) 50, 1429
SduI GDGCHC 6 cut(s) 468, 530, 2190, 2475, 2698, 2941
SfcI CTRYAG 2 cut(s) 1745, 1884
Sfr303I CCGCGG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 1961
SgrBI CCGCGG 1 cut(s) 64
SinI GGWCC 8 cut(s) 520, 763, 849, 904, 954, 1622, 2110, 2347
SmiMI CAYNNNNRTG 2 cut(s) 920, 2676
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SspI AATATT 3 cut(s) 1593, 2776, 3052
SspMI CTAG 6 cut(s) 1182, 1212, 1290, 2027, 2676, 2942
SstI GAGCTC 1 cut(s) 530
StyI CCWWGG 7 cut(s) 714, 957, 1181, 1517, 2003, 2076, 2397
TaaI ACNGT 8 cut(s) 1397, 1645, 1690, 1779, 1852, 2033, 2414, 2835
TaqI TCGA 9 cut(s) 72, 156, 213, 614, 1003, 1091, 1961, 2217, 2970
TaqII GACCGA 4 cut(s) 328, 508, 1087, 2501
TatI WGTACW 6 cut(s) 1575, 1690, 1774, 1792, 2133, 2637
TfiI GAWTC 8 cut(s) 992, 1088, 1373, 1611, 1954, 2023, 2360, 2578
Tru1I TTAA 5 cut(s) 1890, 2061, 2531, 2837, 2904
Tru9I TTAA 5 cut(s) 1890, 2061, 2531, 2837, 2904
TseFI GTSAC 7 cut(s) 164, 442, 1077, 1397, 1684, 2880, 2909
TseI GCWGC 6 cut(s) 781, 970, 1522, 1747, 1904, 2949
Tsp45I GTSAC 7 cut(s) 164, 442, 1077, 1397, 1684, 2880, 2909
TspGWI ACGGA 6 cut(s) 428, 434, 776, 1376, 1586, 2645
VneI GTGCAC 1 cut(s) 2186
VpaK11BI GGWCC 8 cut(s) 520, 763, 849, 904, 954, 1622, 2110, 2347
XapI RAATTY 4 cut(s) 862, 911, 2614, 2795
XbaI TCTAGA 2 cut(s) 1289, 2026
XceI RCATGY 2 cut(s) 2224, 2279
XcmI CCANNNNNNNNNTGG 3 cut(s) 1944, 2149, 2872
XmaJI CCTAGG 1 cut(s) 1181
XmiI GTMKAC 3 cut(s) 614, 2685, 2809
XspI CTAG 6 cut(s) 1182, 1212, 1290, 2027, 2676, 2942
ZraI GACGTC 1 cut(s) 1384
ZrmI AGTACT 1 cut(s) 2135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.