RchiOBHm_Chr5g0061821

PAS fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004648
Physical Location & Seq
Forward (+)
67446775 .. 67457620
10846 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33816

Sequence Viewer

Length: 2829 bp
ATGGATGGCCCTCAAGGACTTCCCCCAACTCCACCGCCGCCGTCCAAGCCTTCCAAACAGTCCCAGCAATCCCCGCCGCTCATCCAGCAGACCATCTCCGCCTCGGTCAACGACAAGCCAACGCCGGAGCAGAGCGACGCCGCGCAGAGGGCCGCCGAGTGGGGATTGGTTCTGAAGACGGACACGGAGACGGGGAAGCCGCAGGGAGTGACCACCCGGAACTCGGGCGGACCCGAAGACCCGAGCCACAAACCGGGTACCTCCAGAAGAAACTCCAACAACTCGGTCCGGAGCTCCGGGGAGTTGTCTGACGACGGCGGCGGCGGAGGAGGAGGAAAAGGGTTTCCGAGAGCTTCGGAGGACTTGAAGAACGTGCTGTCGACGTTTCAGCAGACGTTCGTGGTGTCGGATGCTACCAAACCCGATTACCCGATCATGTACGCCAGCGCCGGGTTTTTCAAGATGACCGGGTACACCTCCAAGGAGGTCATCGGCAGGAACTGCCGGTTTTTGCAGGGAGCGGATACGGACCCGGACGACGTGGCGCAGCTGAGGGAGGCTTTGCAGAAGAACACGAGCTATTGCGGTCGGTTGTTGAATTATAAGAAAGATGGGACCCCTTTTTGGAATTTACTTACTATTGCTCCTATCAAGGACGAAACTGGGAAGGTCCTCAAATTCATTGGAATGCAAGTGGAGGTGAGCAAGCACACTGAGGGGTCCAAGGATAAGATGCTGCGCCCCAATGGATTGCCTGAATCTCTCATTCGATATGATGCTCGTCAGAAAGAGATGGCTACACATTCAGTAACTGAGCTTGTGCAAGCAGTGAGGAGACCGAGGTCACTTGGTGAATCGACAAATCGTCCCTTTAGAAAGTCCGGAGGTGGCGGTAGGGGAGAGGAGGTAATAGAAGCTCTTGCGAGGAGAAACTCCGAGAGTTTGCCTAGGAGAAACTCAGAGAATGTGGCACCACCTAGACGTAACTCACTTGGGGATGCTAAAATCTCAATGCAGTGTATCAATGAGGTTCCTGAAAAGAAACAGAAGAAATCTAGACGGCGCTCTTTCATGGGGGTTATTCGGAAAAGTCAAACTCAAAGCCAAACCGATGATGATACATTTGACAACTTTGGAGATTCTGAGAATGTCCGTGATGACAGTGACAATGATGAGGGACCTATGAGTCTTGAAGATAAAGCGAGGAAGAAGGAAATGAGGAAGGGTATGGATCTTGCTACGACGCTGGAACGTATAGAGAAAAACTTTGTCATTACTGATCCAAGGCTGCCTGATAATCCCATTATATTTGCATCCGATAGCTTCTTAGAGCTAACGGAGTACAGCCGCGAGGAAATATTGGGAAGAAATTGCAGATTCCTTCAAGGACCTGAGACTGATCCAGTAACTGTGAAAAAAATAAGAGAGGCAATTGACAACCAGACTGACGTGACTGTACAACTCATCAATTACACGAAAAGCGGCAAAAAATTCTGGAATGTCTTTCATCTGCAGCCTATGCGTGATCAAAAGGGAGAAGTACAGTATTTTATTGGAGTACAACTTGATGGCAGTCAGCACATTGAGCCTCTGCATAACTCTATTCCTGAAGCTACAGCAAAAGAGAGTGAAAAACTGGTCAAAGAGACTGCAGTTAATGTTGATGAGGCGGCAAGAGAACTTCCAGATGCCAATATGAAACCAGAAGATTTATGGATGAATCATTCGAAGGTCGTTCATCCAAAGCCTCATAGAAGGGACAGCCCCCTTGGAGAGCTATTCAAAAGATTGATTCTAGACAGTGGAGAACAGATAGGATTGAAACATTTTAAGCCTATAAGGCCCTTGGGATCTGGTGATACTGGCAGTGTGCATTTGGTCCAATTGTGTGGAACTGATCAGTACTACGCCATGAAGGCTATGGATAAGGGTATCATGCTCAATAGGAACAAGGTGCACAGAGCTTGTGCGGAGAGAGAAATCCTCGACATGTTGGATCACCCTTTTCTTCCTGCATTGTATGCTTCGTTTCAGACCAAAACACATGTTTGCCTGATCACTGATTACTACCCTGGTGGAGAACTCTTCCTACTTTTGGACAGCCAACCAACGAAGGTCCTGAAGGAAGATTCAGTGAGATTTTATATTGCAGAGGTAGTTGTTGCCTTGGAGTATCTTCACTGTCAAGGGATAATTTACAGGGATTTGAAGCCTGAAAATGTTTTACTTCAGAGCAGTGGGCACGTAACCCTGACCGATTTTGATTTATCGTGTTTGACATCTTGCAAACCACAGCTTTTAATTCCTACCATAAACGAAAAGAAAAGGCATCACAAAGGTCAACAGAATCCAATCTTCATGGCTGAACCAATGAGAGCATCAAATTCTTTTGTTGGCACGGAAGAGTACATCGCTCCGGAAATCATAACTGGAGCTGGCCATTCTAGTGCAGTAGACTGGTGGGCTCTTGGTATTCTTATATATGAAATGCTCTATGGATATACACCATTTAGGGGAAAGACAAGGCAAAAGACGTTCGCCAATATTCTTCACAAGGATCTCAAATTTCCAGGAAGTATACCGGCAAGTCTCCAAGCAAAACAGTTAATGTATCGGTTGTTGCATCGTGATCCCAGAAACAGATTGGGGTCTCAGGAAGGGGCAAGCGAAATTAAGCGTCACCCTTTCTTTCGGGGTGTCAACTGGGCTCTAGTTCGCTGCATGAGCCCTCCCCAACTCGATGCTCCTCTCGTCGGGACAACTGAAGCTGAAAAGGGTGCTAAGCTTGTGGATCCTGAGATGCAAGATCTACAAACAAATATCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

942

Amino Acids

105.44

Weight (kDa)

8.24

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 139 - 235 3e-20 PAS domain
PAS PF00989 144 - 230 6.6e-08 PAS fold
PAS_3 PF08447 145 - 229 2.3e-07 PAS fold
PAS_9 PF13426 431 - 523 9.2e-20 PAS domain
PAS PF00989 434 - 523 1.4e-06 PAS fold
PAS_3 PF08447 435 - 520 5.3e-07 PAS fold
Pkinase PF00069 612 - 897 1.1e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 614 - 759 1.3e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 802 - 882 7.3e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AasI GACNNNNNNGTC 1 cut(s) 1185
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 2 cut(s) 257, 970
AccBSI CCGCTC 2 cut(s) 79, 521
AccI GTMKAC 3 cut(s) 380, 2454, 2578
AccII CGCG 2 cut(s) 143, 1350
AccIII TCCGGA 3 cut(s) 288, 881, 2416
AclWI GGATC 9 cut(s) 1239, 1274, 1394, 1858, 2004, 2565, 2624, 2786, 2799
AcoI YGGCCR 1 cut(s) 2437
AcsI RAATTY 5 cut(s) 628, 677, 1490, 2383, 2564
AcuI CTGAAG 5 cut(s) 194, 1629, 2141, 2213, 2784
AcyI GRCGYC 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 9 cut(s) 259, 440, 473, 1343, 1458, 1542, 1560, 1904, 2408
AfiI CCNNNNNNNGG 9 cut(s) 147, 159, 223, 253, 450, 624, 2095, 2513, 2753
AflIII ACRYGT 2 cut(s) 1989, 2044
AgsI TTSAA 8 cut(s) 367, 460, 598, 1193, 1385, 1783, 1822, 2209
AhdI GACNNNNNGTC 1 cut(s) 864
AjiI CACGTC 2 cut(s) 541, 1450
AjnI CCWGG 2 cut(s) 2071, 2569
Alw21I GWGCWC 2 cut(s) 296, 1959
Alw26I GTCTC 6 cut(s) 182, 829, 1388, 1640, 2594, 2655
Alw44I GTGCAC 1 cut(s) 1955
AlwI GGATC 9 cut(s) 1239, 1274, 1394, 1858, 2004, 2565, 2624, 2786, 2799
AlwNI CAGNNNCTG 3 cut(s) 501, 812, 1409
Ama87I CYCGRG 2 cut(s) 223, 241
Aor13HI TCCGGA 3 cut(s) 288, 881, 2416
AoxI GGCC 4 cut(s) 7, 150, 1841, 2437
ApaLI GTGCAC 1 cut(s) 1955
ApeKI GCWGC 5 cut(s) 547, 736, 1288, 1513, 2718
ApoI RAATTY 5 cut(s) 628, 677, 1490, 2383, 2564
Asp718I GGTACC 1 cut(s) 257
AspA2I CCTAGG 1 cut(s) 947
AspLEI GCGC 5 cut(s) 145, 449, 547, 741, 1065
AsuC2I CCSGG 6 cut(s) 217, 255, 298, 451, 469, 533
AsuHPI GGTGA 5 cut(s) 712, 863, 1868, 1991, 2672
AsuII TTCGAA 1 cut(s) 1727
AvaI CYCGRG 2 cut(s) 223, 241
AvrII CCTAGG 1 cut(s) 947
BaeGI GKGCMC 2 cut(s) 1959, 2244
BalI TGGCCA 1 cut(s) 2439
BamHI GGATCC 1 cut(s) 2791
BanI GGYRCC 2 cut(s) 257, 970
BanII GRGCYC 4 cut(s) 296, 2467, 2710, 2729
BauI CACGAG 1 cut(s) 574
BbsI GAAGAC 2 cut(s) 182, 243
Bbv12I GWGCWC 2 cut(s) 296, 1959
BbvCI CCTCAGC 1 cut(s) 551
BbvI GCAGC 5 cut(s) 559, 723, 1275, 1525, 2705
BccI CCATC 4 cut(s) 101, 605, 787, 1562
BceAI ACGGC 3 cut(s) 25, 331, 1076
BciT130I CCWGG 2 cut(s) 2073, 2571
BciVI GTATCC 1 cut(s) 517
BclI TGATCA 3 cut(s) 1525, 1897, 2055
BcnI CCSGG 6 cut(s) 217, 255, 298, 451, 469, 533
BcoDI GTCTC 6 cut(s) 182, 829, 1388, 1640, 2594, 2655
BfaI CTAG 6 cut(s) 948, 978, 1056, 1796, 2445, 2711
BfmI CTRYAG 3 cut(s) 1511, 1614, 1650
BfoI RGCGCY 2 cut(s) 450, 1066
BfuI GTATCC 1 cut(s) 517
BglI GCCNNNNNGGC 2 cut(s) 1840, 1916
BglII AGATCT 1 cut(s) 2806
BlnI CCTAGG 1 cut(s) 947
BlpI GCTNAGC 1 cut(s) 2781
BmcAI AGTACT 1 cut(s) 1904
Bme1390I CCNGG 8 cut(s) 217, 255, 298, 451, 469, 533, 2073, 2571
BmeRI GACNNNNNGTC 1 cut(s) 864
BmeT110I CYCGRG 2 cut(s) 223, 241
BmgBI CACGTC 2 cut(s) 541, 1450
BmrFI CCNGG 8 cut(s) 217, 255, 298, 451, 469, 533, 2073, 2571
BmrI ACTGGG 2 cut(s) 672, 2713
BmuI ACTGGG 2 cut(s) 672, 2713
BoxI GACNNNNGTC 1 cut(s) 841
BpiI GAAGAC 2 cut(s) 182, 243
BplI GAGNNNNNCTC 2 cut(s) 1968, 2000
BpmI CTGGAG 2 cut(s) 247, 2451
Bpu10I CCTNAGC 1 cut(s) 551
Bpu1102I GCTNAGC 1 cut(s) 2781
Bpu14I TTCGAA 1 cut(s) 1727
BpuMI CCSGG 6 cut(s) 217, 255, 298, 451, 469, 533
BsaAI YACGTR 1 cut(s) 2245
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 2 cut(s) 829, 2655
BsaWI WCCGGW 3 cut(s) 288, 881, 2416
BsaXI ACNNNNNCTCC 4 cut(s) 628, 658, 891, 921
Bsc4I CCNNNNNNNGG 9 cut(s) 147, 159, 223, 253, 450, 624, 2095, 2513, 2753
Bse118I RCCGGY 2 cut(s) 504, 2581
Bse1I ACTGG 7 cut(s) 667, 1403, 1641, 1867, 2434, 2462, 2708
BseAI TCCGGA 3 cut(s) 288, 881, 2416
BseBI CCWGG 2 cut(s) 2073, 2571
BseGI GGATG 7 cut(s) 10, 81, 415, 1003, 1313, 1722, 1738
BseLI CCNNNNNNNGG 9 cut(s) 147, 159, 223, 253, 450, 624, 2095, 2513, 2753
BseMII CTCAG 8 cut(s) 542, 705, 804, 972, 1134, 1383, 2666, 2787
BseNI ACTGG 7 cut(s) 667, 1403, 1641, 1867, 2434, 2462, 2708
BseRI GAGGAG 6 cut(s) 342, 345, 847, 917, 940, 2736
BseSI GKGCMC 2 cut(s) 1959, 2244
BseXI GCAGC 5 cut(s) 559, 723, 1275, 1525, 2705
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 2469
Bsh1236I CGCG 2 cut(s) 143, 1350
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 4 cut(s) 9, 152, 1843, 2439
BshNI GGYRCC 2 cut(s) 257, 970
BsiEI CGRYCG 1 cut(s) 589
BsiHKAI GWGCWC 2 cut(s) 296, 1959
BsiHKCI CYCGRG 2 cut(s) 223, 241
BslFI GGGAC 6 cut(s) 46, 628, 852, 1191, 1772, 2770
BslI CCNNNNNNNGG 9 cut(s) 147, 159, 223, 253, 450, 624, 2095, 2513, 2753
BsmAI GTCTC 6 cut(s) 182, 829, 1388, 1640, 2594, 2655
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 6 cut(s) 46, 628, 852, 1191, 1772, 2770
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 4 cut(s) 9, 152, 1843, 2439
Bso31I GGTCTC 2 cut(s) 829, 2655
BsoBI CYCGRG 2 cut(s) 223, 241
Bsp119I TTCGAA 1 cut(s) 1727
Bsp1286I GDGCHC 6 cut(s) 296, 1959, 2244, 2467, 2710, 2729
Bsp13I TCCGGA 3 cut(s) 288, 881, 2416
Bsp1407I TGTACA 1 cut(s) 1456
Bsp1720I GCTNAGC 1 cut(s) 2781
BspANI GGCC 4 cut(s) 9, 152, 1843, 2439
BspCNI CTCAG 8 cut(s) 543, 706, 805, 971, 1135, 1384, 2665, 2788
BspEI TCCGGA 3 cut(s) 288, 881, 2416
BspFNI CGCG 2 cut(s) 143, 1350
BspMAI CTGCAG 2 cut(s) 1515, 1654
BspPI GGATC 9 cut(s) 1239, 1274, 1394, 1858, 2004, 2565, 2624, 2786, 2799
BspT104I TTCGAA 1 cut(s) 1727
BspT107I GGYRCC 2 cut(s) 257, 970
BspTNI GGTCTC 2 cut(s) 829, 2655
BsrBI CCGCTC 2 cut(s) 79, 521
BsrFI RCCGGY 2 cut(s) 504, 2581
BsrGI TGTACA 1 cut(s) 1456
BsrI ACTGG 7 cut(s) 667, 1403, 1641, 1867, 2434, 2462, 2708
BssAI RCCGGY 2 cut(s) 504, 2581
BssNAI GTATAC 1 cut(s) 2579
BssNI GRCGYC 1 cut(s) 138
BssSI CACGAG 1 cut(s) 574
BssT1I CCWWGG 7 cut(s) 480, 723, 947, 1283, 1768, 1845, 2166
Bst1107I GTATAC 1 cut(s) 2579
Bst2BI CACGAG 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 2073, 2571
Bst4CI ACNGT 8 cut(s) 60, 1163, 1411, 1456, 1545, 1802, 2183, 2604
Bst6I CTCTTC 2 cut(s) 2090, 2397
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 3 cut(s) 501, 1519, 2021
BstAUI TGTACA 1 cut(s) 1456
BstBAI YACGTR 1 cut(s) 2245
BstBI TTCGAA 1 cut(s) 1727
BstC8I GCNNGC 5 cut(s) 445, 707, 825, 2437, 2665
BstF5I GGATG 7 cut(s) 10, 81, 415, 1003, 1313, 1722, 1738
BstFNI CGCG 2 cut(s) 143, 1350
BstH2I RGCGCY 2 cut(s) 450, 1066
BstHHI GCGC 5 cut(s) 145, 449, 547, 741, 1065
BstMAI GTCTC 6 cut(s) 182, 829, 1388, 1640, 2594, 2655
BstMCI CGRYCG 1 cut(s) 589
BstNI CCWGG 2 cut(s) 2073, 2571
BstNSI RCATGY 2 cut(s) 1993, 2048
BstPAI GACNNNNGTC 1 cut(s) 841
BstSCI CCNGG 8 cut(s) 215, 253, 296, 449, 467, 531, 2071, 2569
BstSFI CTRYAG 3 cut(s) 1511, 1614, 1650
BstSLI GKGCMC 2 cut(s) 1959, 2244
BstUI CGCG 2 cut(s) 143, 1350
BstV1I GCAGC 5 cut(s) 559, 723, 1275, 1525, 2705
BstV2I GAAGAC 2 cut(s) 182, 243
BstX2I RGATCY 5 cut(s) 1231, 1850, 2557, 2791, 2806
BstXI CCANNNNNNTGG 1 cut(s) 1889
BstYI RGATCY 5 cut(s) 1231, 1850, 2557, 2791, 2806
BstZ17I GTATAC 1 cut(s) 2579
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 4 cut(s) 9, 152, 1843, 2439
BtgZI GCGATG 1 cut(s) 2395
BtrI CACGTC 2 cut(s) 541, 1450
BtsCI GGATG 7 cut(s) 10, 81, 415, 1003, 1313, 1722, 1738
BtsI GCAGTG 4 cut(s) 834, 1022, 1873, 2242
Cac8I GCNNGC 5 cut(s) 445, 707, 825, 2437, 2665
CaiI CAGNNNCTG 3 cut(s) 501, 812, 1409
CfoI GCGC 5 cut(s) 145, 449, 547, 741, 1065
Cfr10I RCCGGY 2 cut(s) 504, 2581
CpoI CGGWCCG 1 cut(s) 286
CseI GACGC 3 cut(s) 146, 1252, 2666
Csp6I GTAC 9 cut(s) 258, 439, 472, 1342, 1457, 1541, 1559, 1903, 2407
CspI CGGWCCG 1 cut(s) 286
CviAII CATG 8 cut(s) 436, 1072, 1912, 1936, 1990, 2045, 2359, 2722
CviQI GTAC 9 cut(s) 258, 439, 472, 1342, 1457, 1541, 1559, 1903, 2407
DraIII CACNNNGTG 1 cut(s) 851
DrdI GACNNNNNNGTC 1 cut(s) 1185
DriI GACNNNNNGTC 1 cut(s) 864
DseDI GACNNNNNNGTC 1 cut(s) 1185
EaeI YGGCCR 1 cut(s) 2437
Eam1104I CTCTTC 2 cut(s) 2090, 2397
Eam1105I GACNNNNNGTC 1 cut(s) 864
EarI CTCTTC 2 cut(s) 2090, 2397
EciI GGCGGA 3 cut(s) 88, 243, 339
Ecl136II GAGCTC 1 cut(s) 294
Eco130I CCWWGG 7 cut(s) 480, 723, 947, 1283, 1768, 1845, 2166
Eco24I GRGCYC 4 cut(s) 296, 2467, 2710, 2729
Eco31I GGTCTC 2 cut(s) 829, 2655
Eco53kI GAGCTC 1 cut(s) 294
Eco57I CTGAAG 5 cut(s) 194, 1629, 2141, 2213, 2784
Eco88I CYCGRG 2 cut(s) 223, 241
EcoICRI GAGCTC 1 cut(s) 294
EcoO109I RGGNCCY 6 cut(s) 615, 670, 1178, 1388, 1842, 2116
EcoRII CCWGG 2 cut(s) 2071, 2569
EcoT14I CCWWGG 7 cut(s) 480, 723, 947, 1283, 1768, 1845, 2166
EcoT38I GRGCYC 4 cut(s) 296, 2467, 2710, 2729
ErhI CCWWGG 7 cut(s) 480, 723, 947, 1283, 1768, 1845, 2166
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 8 cut(s) 439, 1075, 1915, 1939, 1993, 2048, 2362, 2725
FaqI GGGAC 6 cut(s) 46, 628, 852, 1191, 1772, 2770
FatI CATG 8 cut(s) 435, 1071, 1911, 1935, 1989, 2044, 2358, 2721
FauI CCCGC 1 cut(s) 81
FbaI TGATCA 3 cut(s) 1525, 1897, 2055
FblI GTMKAC 3 cut(s) 380, 2454, 2578
FokI GGATG 7 cut(s) 17, 68, 422, 1010, 1300, 1725, 1729
FriOI GRGCYC 4 cut(s) 296, 2467, 2710, 2729
FspBI CTAG 6 cut(s) 948, 978, 1056, 1796, 2445, 2711
GlaI GCGC 5 cut(s) 144, 448, 546, 740, 1064
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 2 cut(s) 247, 2451
HaeII RGCGCY 2 cut(s) 450, 1066
HaeIII GGCC 4 cut(s) 9, 152, 1843, 2439
HgaI GACGC 3 cut(s) 146, 1252, 2666
HhaI GCGC 5 cut(s) 145, 449, 547, 741, 1065
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 8 cut(s) 439, 1075, 1915, 1939, 1993, 2048, 2362, 2725
Hin6I GCGC 5 cut(s) 143, 447, 545, 739, 1063
HinP1I GCGC 5 cut(s) 143, 447, 545, 739, 1063
HincII GTYRAC 4 cut(s) 109, 381, 2342, 2701
HindII GTYRAC 4 cut(s) 109, 381, 2342, 2701
HindIII AAGCTT 1 cut(s) 2783
HinfI GANTC 9 cut(s) 758, 854, 1139, 1186, 1377, 1720, 1792, 2129, 2347
HphI GGTGA 5 cut(s) 712, 863, 1868, 1991, 2672
Hpy166II GTNNAC 8 cut(s) 109, 381, 474, 1957, 2342, 2455, 2579, 2701
Hpy8I GTNNAC 8 cut(s) 109, 381, 474, 1957, 2342, 2455, 2579, 2701
Hpy99I CGWCG 6 cut(s) 140, 317, 385, 542, 1246, 2756
HpyCH4III ACNGT 8 cut(s) 60, 1163, 1411, 1456, 1545, 1802, 2183, 2604
HpyCH4IV ACGT 9 cut(s) 372, 383, 395, 540, 982, 1252, 1449, 2244, 2534
HpySE526I ACGT 9 cut(s) 372, 383, 395, 540, 982, 1252, 1449, 2244, 2534
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 8 cut(s) 439, 1075, 1915, 1939, 1993, 2048, 2362, 2725
HspAI GCGC 5 cut(s) 143, 447, 545, 739, 1063
KflI GGGWCCC 1 cut(s) 615
Kpn2I TCCGGA 3 cut(s) 288, 881, 2416
KpnI GGTACC 1 cut(s) 261
Ksp22I TGATCA 3 cut(s) 1525, 1897, 2055
LmnI GCTCC 8 cut(s) 127, 291, 299, 518, 649, 2419, 2432, 2749
Lsp1109I GCAGC 5 cut(s) 559, 723, 1275, 1525, 2705
MaeI CTAG 6 cut(s) 948, 978, 1056, 1796, 2445, 2711
MaeII ACGT 9 cut(s) 372, 383, 395, 540, 982, 1252, 1449, 2244, 2534
MaeIII GTNAC 9 cut(s) 208, 808, 843, 983, 1163, 1405, 1450, 2245, 2678
MbiI CCGCTC 2 cut(s) 79, 521
MfeI CAATTG 2 cut(s) 1431, 1883
MflI RGATCY 5 cut(s) 1231, 1850, 2557, 2791, 2806
MhlI GDGCHC 6 cut(s) 296, 1959, 2244, 2467, 2710, 2729
MlsI TGGCCA 1 cut(s) 2439
MluNI TGGCCA 1 cut(s) 2439
MlyI GAGTC 1 cut(s) 1195
MmeI TCCRAC 3 cut(s) 300, 387, 1974
Mox20I TGGCCA 1 cut(s) 2439
MroI TCCGGA 3 cut(s) 288, 881, 2416
MscI TGGCCA 1 cut(s) 2439
MseI TTAA 5 cut(s) 1656, 1830, 2300, 2606, 2673
MslI CAYNNNNRTG 2 cut(s) 686, 2445
Msp20I TGGCCA 1 cut(s) 2439
MspA1I CMGCKG 1 cut(s) 550
MspR9I CCNGG 8 cut(s) 217, 255, 298, 451, 469, 533, 2073, 2571
MunI CAATTG 2 cut(s) 1431, 1883
Mva1269I GAATGC 1 cut(s) 693
MvaI CCWGG 2 cut(s) 2073, 2571
MvnI CGCG 2 cut(s) 143, 1350
NciI CCSGG 6 cut(s) 217, 255, 298, 451, 469, 533
NlaIII CATG 8 cut(s) 439, 1075, 1915, 1939, 1993, 2048, 2362, 2725
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 5 cut(s) 208, 843, 1163, 1450, 2678
NspI RCATGY 2 cut(s) 1993, 2048
NspV TTCGAA 1 cut(s) 1727
PciI ACATGT 2 cut(s) 1989, 2044
PctI GAATGC 1 cut(s) 693
PfeI GAWTC 8 cut(s) 758, 854, 1139, 1377, 1720, 1792, 2129, 2347
PfoI TCCNGGA 1 cut(s) 2569
PleI GAGTC 1 cut(s) 1194
PpsI GAGTC 1 cut(s) 1194
Ppu21I YACGTR 1 cut(s) 2245
PpuMI RGGWCCY 5 cut(s) 615, 670, 1178, 1388, 2116
PscI ACATGT 2 cut(s) 1989, 2044
PshAI GACNNNNGTC 1 cut(s) 841
PsiI TTATAA 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 296
Psp5II RGGWCCY 5 cut(s) 615, 670, 1178, 1388, 2116
Psp6I CCWGG 2 cut(s) 2071, 2569
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 2 cut(s) 2071, 2569
PspPPI RGGWCCY 5 cut(s) 615, 670, 1178, 1388, 2116
PsrI GAACNNNNNNTAC 2 cut(s) 2073, 2105
PstI CTGCAG 2 cut(s) 1515, 1654
PstNI CAGNNNCTG 3 cut(s) 501, 812, 1409
PsuI RGATCY 5 cut(s) 1231, 1850, 2557, 2791, 2806
PvuII CAGCTG 1 cut(s) 550
RsaI GTAC 9 cut(s) 259, 440, 473, 1343, 1458, 1542, 1560, 1904, 2408
RsaNI GTAC 9 cut(s) 258, 439, 472, 1342, 1457, 1541, 1559, 1903, 2407
RseI CAYNNNNRTG 2 cut(s) 686, 2445
Rsr2I CGGWCCG 1 cut(s) 286
RsrII CGGWCCG 1 cut(s) 286
SacI GAGCTC 1 cut(s) 296
SalI GTCGAC 1 cut(s) 379
SaqAI TTAA 5 cut(s) 1656, 1830, 2300, 2606, 2673
ScaI AGTACT 1 cut(s) 1904
SchI GAGTC 1 cut(s) 1195
ScrFI CCNGG 8 cut(s) 217, 255, 298, 451, 469, 533, 2073, 2571
SduI GDGCHC 6 cut(s) 296, 1959, 2244, 2467, 2710, 2729
SfcI CTRYAG 3 cut(s) 1511, 1614, 1650
SfuI TTCGAA 1 cut(s) 1727
SmiMI CAYNNNNRTG 2 cut(s) 686, 2445
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SspI AATATT 2 cut(s) 1359, 2545
SspMI CTAG 6 cut(s) 948, 978, 1056, 1796, 2445, 2711
SstI GAGCTC 1 cut(s) 296
StyD4I CCNGG 8 cut(s) 215, 253, 296, 449, 467, 531, 2071, 2569
StyI CCWWGG 7 cut(s) 480, 723, 947, 1283, 1768, 1845, 2166
TaaI ACNGT 8 cut(s) 60, 1163, 1411, 1456, 1545, 1802, 2183, 2604
TaiI ACGT 9 cut(s) 375, 386, 398, 543, 985, 1255, 1452, 2247, 2537
TaqI TCGA 6 cut(s) 380, 769, 857, 1727, 1986, 2739
TaqII GACCGA 4 cut(s) 94, 274, 853, 2270
TatI WGTACW 6 cut(s) 1341, 1456, 1540, 1558, 1902, 2406
TfiI GAWTC 8 cut(s) 758, 854, 1139, 1377, 1720, 1792, 2129, 2347
Tru1I TTAA 5 cut(s) 1656, 1830, 2300, 2606, 2673
Tru9I TTAA 5 cut(s) 1656, 1830, 2300, 2606, 2673
TseFI GTSAC 5 cut(s) 208, 843, 1163, 1450, 2678
TseI GCWGC 5 cut(s) 547, 736, 1288, 1513, 2718
Tsp45I GTSAC 5 cut(s) 208, 843, 1163, 1450, 2678
TspDTI ATGAA 9 cut(s) 670, 1060, 1496, 1712, 1727, 1733, 1928, 2347, 2499
TspGWI ACGGA 6 cut(s) 194, 200, 542, 1142, 1352, 2414
VneI GTGCAC 1 cut(s) 1955
XapI RAATTY 5 cut(s) 628, 677, 1490, 2383, 2564
XbaI TCTAGA 2 cut(s) 1055, 1795
XceI RCATGY 2 cut(s) 1993, 2048
XcmI CCANNNNNNNNNTGG 3 cut(s) 1710, 1918, 2641
XmaJI CCTAGG 1 cut(s) 947
XmiI GTMKAC 3 cut(s) 380, 2454, 2578
XspI CTAG 6 cut(s) 948, 978, 1056, 1796, 2445, 2711
ZrmI AGTACT 1 cut(s) 1904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.