FvH4_3g40860

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005865
Physical Location & Seq
Reverse (-)
34411025 .. 34413969
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40860.t3

Sequence Viewer

Length: 1746 bp
ATGGCCTCTCTTGCCCAAAATAACAGCAGGCGCCTTTCCATAGCCATGGAGCGAACTGGCCAATGGATTTTCTCTCAAGATATCCCTGCAGATGTAGTAGTCGAAGTTGGCGAAGCCAATTTCTCATTACACAAGTTCATGCTAGTGGCCAAGAGCAACCGAATAAGGCAACTGATACTGGAATCCGACGAACCAGACCTGACCCGAATCAACCTCTCCGACATTCCTGGAGGACCAGAGACGTTCGAGAAGGCAGCCAAGTTCTGCTACGGTGTCAACTTCGAAATCACAGTCCACAACGTAGCTGCTCTCCGATGCGCCGCCGAGTACCTCCAAATGACTGATAAGTACTGCGACAACAACCTCAGCGGCCGTACAGAGGATTTCCTCACTCAGGTCGCCTTGTCCAGCCTCTCCGGCGCCATTGTCGTCCTCAAATCGTGTGAAGGCCTCACTCCCATGGCTGAGGACCTCAAAATCGTTCAGAGATGTGTAGAAGTGGTCACTTCTAAGGTTACAATAGAGGCAAACTTTCCGAGCAGAGCGCCGACGAACTGGTGGACGGAGGAGCTATCTATTTTGGACATTGAATTCTTTGGGAGAGTTATAAAATCCATGAAGCAACGTGCTTCGAAAACTCTCACCGTATCGAGCGCGATCGTTACATACGCAGAGAGATGGCTCCGGGATTTGATCCGGGACCACTCCGGCGCCGGCACCAAGGCCTTGGACTCCGACGACTCCGACGTCAGAGTCCACCAGCGGGAACTTATACAGTCCATAGTGGCTCTCCTTCCTTCCGAGAAGGCCGCTCTACCGATCAACTTCCTCTGCTGCCTTCTTAGATCAGCGACGTTCGTCAAGGCCTCGACGGCAGCCAAGAACGAGCTGGAGAAGCGGATCTCGGCGATCTTGGAGCACGTGACAGTCGACGACCTGCTGGTGTTGTCGTTTACTTACGACGGCGAGAGGCTGTTCGATCTGGAGAGCGTGAGGAGGATTATATCGGGGTTTGTGGAGAGGGAGAAGAGCGTGGCGGTTTTTAACGCCGGTGACTTCAGAGAGGTGTGCTCCGCTGCGATGCTTAGAGTTGCTAAGACTGTGGATGCGTATCTCGGCGAGATAGCCACCTGTACCGAGCTTAGCATATCCAAGTTTAATGGCATCGCCGTCCTCGTCCCCAAAGGCGCGCGTAAAGTCGACGACGATCTCTACCGGGCCGTTGACATATACTTGAAGGCTCATCCAAACCTTGATGAGATAGAGAGGGAGAAAGTGTGCAGTGTAATGGATGCGTTGAAGTTGTCGTACGAAGCGAGATTGCATGCTTCGCAAAATAAGCGTTTGCCGGTACAGATTGTGCTGCATGCGCTCTACTACGACCAGCTCAAGCTAAGGAGCGGCGAAGAGGAGAACGAGACGCAAATTGATGCTGCATCGACGAGAAATCAGGTACAGGCCGACGTGTCGTTGGCTAAGGAAAACGAGGTCCTTAGAACAGAGCTACTGAAGATGAAGATGTACATTTCAGACATAAAAAAGAGTGGAGGTGGTGCGGGTGCAAGTGCAGCGACGACATCGTCTGGGAAAGGCGGTGGGGGGCCGAGAAAGCCGACATTTTTCTCGTCGGTGTCGAAGAAACTGGGGAAGTTGAATCCCTTCAAAAATGGGTCTAAGGATACTTCCAACATTGATGATTGTGTGGACGTTACCAAGCCTAGAAGAAGAAGGTTTTCCATCTCTTAA

Protein Analysis

582

Amino Acids

64.34

Weight (kDa)

7.07

Isoelectric Point (pI)

35.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 139 - 393 1e-76 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 608
AasI GACNNNNNNGTC 3 cut(s) 746, 752, 1579
AatII GACGTC 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 945
AccB1I GGYRCC 4 cut(s) 30, 419, 710, 716
AccBSI CCGCTC 2 cut(s) 812, 1401
AccI GTMKAC 2 cut(s) 930, 1200
AccII CGCG 3 cut(s) 656, 1190, 1192
AclWI GGATC 2 cut(s) 688, 908
AcoI YGGCCR 3 cut(s) 58, 147, 370
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 2 cut(s) 1042, 1529
AcvI CACGTG 1 cut(s) 922
AcyI GRCGYC 4 cut(s) 31, 420, 711, 747
AfaI GTAC 8 cut(s) 329, 350, 376, 1135, 1310, 1353, 1455, 1523
AfiI CCNNNNNNNGG 3 cut(s) 379, 394, 763
AflIII ACRYGT 1 cut(s) 1464
AgsI TTSAA 5 cut(s) 590, 1237, 1300, 1654, 1663
AjiI CACGTC 1 cut(s) 1465
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 7 cut(s) 305, 571, 889, 1141, 1387, 1393, 1504
AluI AGCT 7 cut(s) 305, 571, 889, 1141, 1387, 1393, 1504
Alw21I GWGCWC 2 cut(s) 921, 1073
Alw26I GTCTC 2 cut(s) 233, 1412
AlwI GGATC 2 cut(s) 688, 908
ApeKI GCWGC 8 cut(s) 254, 305, 834, 875, 1076, 1363, 1433, 1568
ApoI RAATTY 1 cut(s) 590
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 2 cut(s) 1658, 1732
AspLEI GCGC 9 cut(s) 33, 320, 422, 547, 656, 713, 1190, 1192, 1372
AspS9I GGNCC 6 cut(s) 233, 469, 700, 1218, 1489, 1601
AsuC2I CCSGG 3 cut(s) 686, 698, 1217
AsuHPI GGTGA 2 cut(s) 634, 1064
AsuII TTCGAA 2 cut(s) 282, 632
AvaII GGWCC 4 cut(s) 233, 469, 700, 1489
BalI TGGCCA 2 cut(s) 60, 149
BanI GGYRCC 4 cut(s) 30, 419, 710, 716
BarI GAAGNNNNNNTAC 2 cut(s) 1292, 1324
BbrPI CACGTG 1 cut(s) 922
Bbv12I GWGCWC 2 cut(s) 921, 1073
BbvCI CCTCAGC 2 cut(s) 365, 465
BbvI GCAGC 8 cut(s) 266, 292, 821, 887, 1063, 1350, 1420, 1580
BccI CCATC 2 cut(s) 672, 1745
BceAI ACGGC 5 cut(s) 357, 888, 979, 1154, 1205
BciT130I CCWGG 1 cut(s) 228
BciVI GTATCC 1 cut(s) 1672
BcnI CCSGG 3 cut(s) 686, 698, 1217
BcoDI GTCTC 2 cut(s) 233, 1412
BfaI CTAG 2 cut(s) 143, 1719
BfmI CTRYAG 1 cut(s) 87
BfoI RGCGCY 4 cut(s) 34, 423, 548, 714
BfuAI ACCTGC 1 cut(s) 945
BfuI GTATCC 1 cut(s) 1672
BglI GCCNNNNNGGC 2 cut(s) 417, 872
BlpI GCTNAGC 1 cut(s) 1142
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 4 cut(s) 228, 686, 698, 1217
Bme18I GGWCC 4 cut(s) 233, 469, 700, 1489
BmgBI CACGTC 1 cut(s) 1465
BmgT120I GGNCC 6 cut(s) 233, 469, 700, 1218, 1489, 1601
BmiI GGNNCC 7 cut(s) 32, 421, 683, 701, 712, 718, 1602
BmrFI CCNGG 4 cut(s) 228, 686, 698, 1217
BmrI ACTGGG 1 cut(s) 1652
BmsI GCATC 7 cut(s) 305, 1071, 1096, 1173, 1282, 1420, 1445
BmuI ACTGGG 1 cut(s) 1652
BoxI GACNNNNGTC 1 cut(s) 857
BplI GAGNNNNNCTC 2 cut(s) 1055, 1087
BpmI CTGGAG 3 cut(s) 249, 911, 1004
Bpu10I CCTNAGC 4 cut(s) 365, 465, 1394, 1476
Bpu1102I GCTNAGC 1 cut(s) 1142
Bpu14I TTCGAA 2 cut(s) 282, 632
BpuEI CTTGAG 2 cut(s) 60, 1373
BpuMI CCSGG 3 cut(s) 686, 698, 1217
BsaAI YACGTR 1 cut(s) 922
BsaBI GATNNNNATC 1 cut(s) 1110
BsaHI GRCGYC 4 cut(s) 31, 420, 711, 747
BsaJI CCNNGG 4 cut(s) 45, 459, 720, 726
BsaXI ACNNNNNCTCC 2 cut(s) 1262, 1292
Bsc4I CCNNNNNNNGG 3 cut(s) 379, 394, 763
Bse118I RCCGGY 3 cut(s) 713, 1049, 1348
Bse1I ACTGG 4 cut(s) 61, 183, 560, 1647
Bse8I GATNNNNATC 1 cut(s) 1110
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 4 cut(s) 45, 459, 720, 726
BseGI GGATG 3 cut(s) 1111, 1243, 1297
BseJI GATNNNNATC 1 cut(s) 1110
BseLI CCNNNNNNNGG 3 cut(s) 379, 394, 763
BseMII CTCAG 3 cut(s) 379, 407, 456
BseNI ACTGG 4 cut(s) 61, 183, 560, 1647
BsePI GCGCGC 1 cut(s) 1188
BseRI GAGGAG 3 cut(s) 581, 1009, 1424
BseX3I CGGCCG 1 cut(s) 370
BseXI GCAGC 8 cut(s) 266, 292, 821, 887, 1063, 1350, 1420, 1580
BsgI GTGCAG 2 cut(s) 1300, 1587
Bsh1236I CGCG 3 cut(s) 656, 1190, 1192
Bsh1285I CGRYCG 2 cut(s) 373, 660
BshNI GGYRCC 4 cut(s) 30, 419, 710, 716
BsiEI CGRYCG 2 cut(s) 373, 660
BsiHKAI GWGCWC 2 cut(s) 921, 1073
BsiSI CCGG 8 cut(s) 417, 685, 697, 708, 714, 1050, 1216, 1349
BsiWI CGTACG 1 cut(s) 1308
BslFI GGGAC 2 cut(s) 713, 1163
BslI CCNNNNNNNGG 3 cut(s) 379, 394, 763
BsmAI GTCTC 2 cut(s) 233, 1412
BsmBI CGTCTC 2 cut(s) 233, 1412
BsmFI GGGAC 2 cut(s) 713, 1163
Bsp119I TTCGAA 2 cut(s) 282, 632
Bsp1286I GDGCHC 2 cut(s) 921, 1073
Bsp1407I TGTACA 1 cut(s) 1521
Bsp143I GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
Bsp1720I GCTNAGC 1 cut(s) 1142
Bsp19I CCATGG 2 cut(s) 45, 459
BspCNI CTCAG 3 cut(s) 378, 406, 457
BspFNI CGCG 3 cut(s) 656, 1190, 1192
BspLI GGNNCC 7 cut(s) 32, 421, 683, 701, 712, 718, 1602
BspMAI CTGCAG 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 945
BspPI GGATC 2 cut(s) 688, 908
BspQI GCTCTTC 1 cut(s) 1022
BspT104I TTCGAA 2 cut(s) 282, 632
BspT107I GGYRCC 4 cut(s) 30, 419, 710, 716
BsrBI CCGCTC 2 cut(s) 812, 1401
BsrFI RCCGGY 3 cut(s) 713, 1049, 1348
BsrGI TGTACA 1 cut(s) 1521
BsrI ACTGG 4 cut(s) 61, 183, 560, 1647
BssAI RCCGGY 3 cut(s) 713, 1049, 1348
BssECI CCNNGG 4 cut(s) 45, 459, 720, 726
BssHII GCGCGC 1 cut(s) 1188
BssMI GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
BssNI GRCGYC 4 cut(s) 31, 420, 711, 747
BssT1I CCWWGG 4 cut(s) 45, 459, 720, 726
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 6 cut(s) 272, 292, 646, 777, 928, 1102
Bst6I CTCTTC 2 cut(s) 1022, 1401
BstACI GRCGYC 4 cut(s) 31, 420, 711, 747
BstAUI TGTACA 1 cut(s) 1521
BstBAI YACGTR 1 cut(s) 922
BstBI TTCGAA 2 cut(s) 282, 632
BstC8I GCNNGC 5 cut(s) 29, 715, 1190, 1326, 1368
BstDSI CCRYGG 2 cut(s) 45, 459
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 3 cut(s) 1111, 1243, 1297
BstFNI CGCG 3 cut(s) 656, 1190, 1192
BstH2I RGCGCY 4 cut(s) 34, 423, 548, 714
BstHHI GCGC 9 cut(s) 33, 320, 422, 547, 656, 713, 1190, 1192, 1372
BstKTI GATC 8 cut(s) 660, 696, 822, 848, 903, 912, 982, 1210
BstMAI GTCTC 2 cut(s) 233, 1412
BstMBI GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
BstMCI CGRYCG 2 cut(s) 373, 660
BstMWI GCNNNNNNNGC 9 cut(s) 11, 417, 872, 895, 1330, 1339, 1369, 1568, 1609
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 2 cut(s) 1328, 1370
BstPAI GACNNNNGTC 1 cut(s) 857
BstSCI CCNGG 4 cut(s) 226, 684, 696, 1215
BstSFI CTRYAG 1 cut(s) 87
BstUI CGCG 3 cut(s) 656, 1190, 1192
BstV1I GCAGC 8 cut(s) 266, 292, 821, 887, 1063, 1350, 1420, 1580
BstX2I RGATCY 1 cut(s) 900
BstXI CCANNNNNNTGG 2 cut(s) 46, 727
BstYI RGATCY 1 cut(s) 900
BstZI CGGCCG 1 cut(s) 370
BsuI GTATCC 1 cut(s) 1672
BtgI CCRYGG 2 cut(s) 45, 459
BtgZI GCGATG 2 cut(s) 1094, 1150
BtrI CACGTC 1 cut(s) 1465
BtsCI GGATG 3 cut(s) 1111, 1243, 1297
BtsI GCAGTG 1 cut(s) 1288
BtsIMutI CAGTG 1 cut(s) 1288
BveI ACCTGC 1 cut(s) 945
Cac8I GCNNGC 5 cut(s) 29, 715, 1190, 1326, 1368
CfoI GCGC 9 cut(s) 33, 320, 422, 547, 656, 713, 1190, 1192, 1372
Cfr10I RCCGGY 3 cut(s) 713, 1049, 1348
Cfr13I GGNCC 6 cut(s) 233, 469, 700, 1218, 1489, 1601
CseI GACGC 1 cut(s) 1429
Csp6I GTAC 8 cut(s) 328, 349, 375, 1134, 1309, 1352, 1454, 1522
CviAII CATG 6 cut(s) 46, 139, 460, 616, 1325, 1367
CviQI GTAC 8 cut(s) 328, 349, 375, 1134, 1309, 1352, 1454, 1522
DinI GGCGCC 3 cut(s) 32, 421, 712
DpnI GATC 8 cut(s) 659, 695, 821, 847, 902, 911, 981, 1209
DpnII GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
DrdI GACNNNNNNGTC 3 cut(s) 746, 752, 1579
DseDI GACNNNNNNGTC 3 cut(s) 746, 752, 1579
EaeI YGGCCR 3 cut(s) 58, 147, 370
EagI CGGCCG 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 1022, 1401
EarI CTCTTC 2 cut(s) 1022, 1401
EclXI CGGCCG 1 cut(s) 370
Eco130I CCWWGG 4 cut(s) 45, 459, 720, 726
Eco147I AGGCCT 3 cut(s) 450, 725, 866
Eco32I GATATC 1 cut(s) 82
Eco47I GGWCC 4 cut(s) 233, 469, 700, 1489
Eco52I CGGCCG 1 cut(s) 370
Eco57I CTGAAG 2 cut(s) 1042, 1529
Eco72I CACGTG 1 cut(s) 922
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoO109I RGGNCCY 2 cut(s) 469, 1489
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 226
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 4 cut(s) 45, 459, 720, 726
EgeI GGCGCC 3 cut(s) 32, 421, 712
EheI GGCGCC 3 cut(s) 32, 421, 712
ErhI CCWWGG 4 cut(s) 45, 459, 720, 726
Esp3I CGTCTC 2 cut(s) 233, 1412
FaeI CATG 6 cut(s) 49, 142, 463, 619, 1328, 1370
FaqI GGGAC 2 cut(s) 713, 1163
FatI CATG 6 cut(s) 45, 138, 459, 615, 1324, 1366
FauI CCCGC 2 cut(s) 756, 1549
FblI GTMKAC 2 cut(s) 930, 1200
FokI GGATG 3 cut(s) 1118, 1230, 1304
FspBI CTAG 2 cut(s) 143, 1719
GlaI GCGC 9 cut(s) 32, 319, 421, 546, 655, 712, 1189, 1191, 1371
GsuI CTGGAG 3 cut(s) 249, 911, 1004
HaeII RGCGCY 4 cut(s) 34, 423, 548, 714
HapII CCGG 8 cut(s) 417, 685, 697, 708, 714, 1050, 1216, 1349
HgaI GACGC 1 cut(s) 1429
HhaI GCGC 9 cut(s) 33, 320, 422, 547, 656, 713, 1190, 1192, 1372
Hin1I GRCGYC 4 cut(s) 31, 420, 711, 747
Hin1II CATG 6 cut(s) 49, 142, 463, 619, 1328, 1370
Hin6I GCGC 9 cut(s) 31, 318, 420, 545, 654, 711, 1188, 1190, 1370
HinP1I GCGC 9 cut(s) 31, 318, 420, 545, 654, 711, 1188, 1190, 1370
HincII GTYRAC 4 cut(s) 277, 931, 1201, 1225
HindII GTYRAC 4 cut(s) 277, 931, 1201, 1225
HinfI GANTC 6 cut(s) 182, 207, 731, 740, 753, 1654
HpaII CCGG 8 cut(s) 417, 685, 697, 708, 714, 1050, 1216, 1349
HphI GGTGA 2 cut(s) 634, 1064
Hpy166II GTNNAC 9 cut(s) 277, 295, 561, 757, 931, 954, 1201, 1225, 1705
Hpy188III TCNNGA 3 cut(s) 77, 247, 983
Hpy8I GTNNAC 9 cut(s) 277, 295, 561, 757, 931, 954, 1201, 1225, 1705
HpyAV CCTTC 9 cut(s) 244, 440, 799, 803, 807, 848, 1231, 1669, 1722
HpyCH4III ACNGT 6 cut(s) 272, 292, 646, 777, 928, 1102
HpyCH4IV ACGT 8 cut(s) 242, 300, 625, 747, 854, 921, 1464, 1707
HpyCH4V TGCA 7 cut(s) 89, 1281, 1324, 1366, 1436, 1562, 1568
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 417, 872, 895, 1330, 1339, 1369, 1568, 1609
HpySE526I ACGT 8 cut(s) 242, 300, 625, 747, 854, 921, 1464, 1707
Hsp92I GRCGYC 4 cut(s) 31, 420, 711, 747
Hsp92II CATG 6 cut(s) 49, 142, 463, 619, 1328, 1370
HspAI GCGC 9 cut(s) 31, 318, 420, 545, 654, 711, 1188, 1190, 1370
KasI GGCGCC 3 cut(s) 30, 419, 710
KroI GCCGGC 1 cut(s) 713
KroNI GCCGGC 1 cut(s) 715
Kzo9I GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
LguI GCTCTTC 1 cut(s) 1022
LmnI GCTCC 6 cut(s) 49, 568, 687, 916, 1076, 1398
Lsp1109I GCAGC 8 cut(s) 266, 292, 821, 887, 1063, 1350, 1420, 1580
LweI GCATC 7 cut(s) 305, 1071, 1096, 1173, 1282, 1420, 1445
MaeI CTAG 2 cut(s) 143, 1719
MaeII ACGT 8 cut(s) 242, 300, 625, 747, 854, 921, 1464, 1707
MaeIII GTNAC 6 cut(s) 502, 514, 661, 922, 1052, 1708
MalI GATC 8 cut(s) 659, 695, 821, 847, 902, 911, 981, 1209
MbiI CCGCTC 2 cut(s) 812, 1401
MboI GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
MboII GAAGA 7 cut(s) 1039, 1418, 1522, 1528, 1648, 1734, 1737
MflI RGATCY 1 cut(s) 900
MhlI GDGCHC 2 cut(s) 921, 1073
MlsI TGGCCA 2 cut(s) 60, 149
MluCI AATT 3 cut(s) 118, 590, 1425
MluNI TGGCCA 2 cut(s) 60, 149
Mly113I GGCGCC 3 cut(s) 31, 420, 711
MlyI GAGTC 3 cut(s) 725, 734, 762
MmeI TCCRAC 5 cut(s) 210, 243, 759, 768, 1710
Mox20I TGGCCA 2 cut(s) 60, 149
MroNI GCCGGC 1 cut(s) 713
MroXI GAANNNNTTC 2 cut(s) 1658, 1732
MscI TGGCCA 2 cut(s) 60, 149
MseI TTAA 3 cut(s) 1044, 1158, 1744
MslI CAYNNNNRTG 3 cut(s) 44, 143, 458
Msp20I TGGCCA 2 cut(s) 60, 149
MspA1I CMGCKG 3 cut(s) 369, 763, 1076
MspI CCGG 8 cut(s) 417, 685, 697, 708, 714, 1050, 1216, 1349
MspR9I CCNGG 4 cut(s) 228, 686, 698, 1217
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 3 cut(s) 656, 1190, 1192
MwoI GCNNNNNNNGC 9 cut(s) 11, 417, 872, 895, 1330, 1339, 1369, 1568, 1609
NaeI GCCGGC 1 cut(s) 715
NarI GGCGCC 3 cut(s) 31, 420, 711
NciI CCSGG 3 cut(s) 686, 698, 1217
NcoI CCATGG 2 cut(s) 45, 459
NdeII GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
NgoMIV GCCGGC 1 cut(s) 713
NlaIII CATG 6 cut(s) 49, 142, 463, 619, 1328, 1370
NlaIV GGNNCC 7 cut(s) 32, 421, 683, 701, 712, 718, 1602
NmeAIII GCCGAG 4 cut(s) 349, 884, 1095, 1629
NmuCI GTSAC 3 cut(s) 502, 922, 1052
NspI RCATGY 2 cut(s) 1328, 1370
NspV TTCGAA 2 cut(s) 282, 632
PaeI GCATGC 2 cut(s) 1328, 1370
PauI GCGCGC 1 cut(s) 1188
PceI AGGCCT 3 cut(s) 450, 725, 866
PciSI GCTCTTC 1 cut(s) 1022
PcsI WCGNNNNNNNCGW 6 cut(s) 108, 744, 927, 1173, 1313, 1631
PdiI GCCGGC 1 cut(s) 715
PdmI GAANNNNTTC 2 cut(s) 1658, 1732
PfeI GAWTC 3 cut(s) 182, 207, 1654
Pfl23II CGTACG 1 cut(s) 1308
PflFI GACNNNGTC 1 cut(s) 1579
PfoI TCCNGGA 3 cut(s) 226, 684, 696
Ple19I CGATCG 1 cut(s) 660
PleI GAGTC 3 cut(s) 725, 734, 761
PluTI GGCGCC 3 cut(s) 34, 423, 714
PmaCI CACGTG 1 cut(s) 922
PmlI CACGTG 1 cut(s) 922
PpsI GAGTC 3 cut(s) 725, 734, 761
Ppu21I YACGTR 1 cut(s) 922
PpuMI RGGWCCY 2 cut(s) 469, 1489
PshAI GACNNNNGTC 1 cut(s) 857
PsiI TTATAA 1 cut(s) 608
Psp5II RGGWCCY 2 cut(s) 469, 1489
Psp6I CCWGG 1 cut(s) 226
PspCI CACGTG 1 cut(s) 922
PspGI CCWGG 1 cut(s) 226
PspLI CGTACG 1 cut(s) 1308
PspN4I GGNNCC 7 cut(s) 32, 421, 683, 701, 712, 718, 1602
PspPI GGNCC 6 cut(s) 233, 469, 700, 1218, 1489, 1601
PspPPI RGGWCCY 2 cut(s) 469, 1489
PstI CTGCAG 1 cut(s) 91
PsuI RGATCY 1 cut(s) 900
PsyI GACNNNGTC 1 cut(s) 1579
PteI GCGCGC 1 cut(s) 1188
PvuI CGATCG 1 cut(s) 660
RsaI GTAC 8 cut(s) 329, 350, 376, 1135, 1310, 1353, 1455, 1523
RsaNI GTAC 8 cut(s) 328, 349, 375, 1134, 1309, 1352, 1454, 1522
RseI CAYNNNNRTG 3 cut(s) 44, 143, 458
SalI GTCGAC 2 cut(s) 929, 1199
SapI GCTCTTC 1 cut(s) 1022
SaqAI TTAA 3 cut(s) 1044, 1158, 1744
Sau3AI GATC 8 cut(s) 657, 693, 819, 845, 900, 909, 979, 1207
Sau96I GGNCC 6 cut(s) 233, 469, 700, 1218, 1489, 1601
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 3 cut(s) 725, 734, 762
ScrFI CCNGG 4 cut(s) 228, 686, 698, 1217
SduI GDGCHC 2 cut(s) 921, 1073
SfaNI GCATC 7 cut(s) 305, 1071, 1096, 1173, 1282, 1420, 1445
SfcI CTRYAG 1 cut(s) 87
SfoI GGCGCC 3 cut(s) 32, 421, 712
SfuI TTCGAA 2 cut(s) 282, 632
SgrAI CRCCGGYG 1 cut(s) 1049
SinI GGWCC 4 cut(s) 233, 469, 700, 1489
SmiMI CAYNNNNRTG 3 cut(s) 44, 143, 458
SmlI CTYRAG 2 cut(s) 75, 1388
SmoI CTYRAG 2 cut(s) 75, 1388
SphI GCATGC 2 cut(s) 1328, 1370
Sse9I AATT 3 cut(s) 118, 590, 1425
SseBI AGGCCT 3 cut(s) 450, 725, 866
SspDI GGCGCC 3 cut(s) 30, 419, 710
SspMI CTAG 2 cut(s) 143, 1719
StuI AGGCCT 3 cut(s) 450, 725, 866
StyD4I CCNGG 4 cut(s) 226, 684, 696, 1215
StyI CCWWGG 4 cut(s) 45, 459, 720, 726
TaaI ACNGT 6 cut(s) 272, 292, 646, 777, 928, 1102
TaiI ACGT 8 cut(s) 245, 303, 628, 750, 857, 924, 1467, 1710
TasI AATT 3 cut(s) 118, 590, 1425
TatI WGTACW 2 cut(s) 348, 1521
TauI GCSGC 4 cut(s) 323, 372, 812, 1404
TfiI GAWTC 3 cut(s) 182, 207, 1654
Tru1I TTAA 3 cut(s) 1044, 1158, 1744
Tru9I TTAA 3 cut(s) 1044, 1158, 1744
TscAI CASTG 1 cut(s) 1288
TseFI GTSAC 3 cut(s) 502, 922, 1052
TseI GCWGC 8 cut(s) 254, 305, 834, 875, 1076, 1363, 1433, 1568
Tsp45I GTSAC 3 cut(s) 502, 922, 1052
TspDTI ATGAA 3 cut(s) 127, 632, 1529
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 1 cut(s) 1288
Tth111I GACNNNGTC 1 cut(s) 1579
VpaK11BI GGWCC 4 cut(s) 233, 469, 700, 1489
XagI CCTNNNNNAGG 1 cut(s) 392
XapI RAATTY 1 cut(s) 590
XceI RCATGY 2 cut(s) 1328, 1370
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmiI GTMKAC 2 cut(s) 930, 1200
XmnI GAANNNNTTC 2 cut(s) 1658, 1732
XspI CTAG 2 cut(s) 143, 1719
ZraI GACGTC 1 cut(s) 748
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.