Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005865

Physical Location & Seq

Genomic Coordinates Forward (+)

3934262 .. 3937799

Gene Structure 3538 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1048900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGGCTTCTTCTCTCAGCGGCGGTAACAGGCTCTCCCTTGCCATGGAGCGCACTGGCCAATGGATTATCTCTCAAGATATTCCGTCAGATGTTGTGGTTGAAGTTGGTGAAGCCAATTTCTCTCTACACAAGTTCATGCTCGTGGCCAAGAGCAACCGCATAAGAAAACTGATAATGGAGTCAAAACAACCGGACCTAACGAGAATCAACCTCTCCGACATTCCCGGAGGCCCCGAGACGTTCGAGAAGGCGGCCAAGTTCTGTTACGGCGTCAACTTCGAGATCACAGTTCACAACGTCGCCGCCCTCCGCTGCGCGGCGGAGTATTTGGAAATGACTGATAAGTACTGCGAGAACAACCTCACCGGCCGCACCGAAGATTTCTTAGCTCAGGTCGCTCTCACGAGCCTCTCCGGCGCCATTGTCGTGCTCAAGACCTGCGAAGACCTCCTTCCCATGGCGGAGGACCTCAAAATTGTACAGAGATGCGTCGATGTCGCCACTTCCAAGGTATCAATCGAAGCGAAATTTCCGAGTCGATCGCCGAAGAATTGGTGGACTGAGGAATTGTCGATTTTGGACATTGAGTTCTTCGGCAGAGTAATCGCCTCGTTGAAGCTGCGCGGCGTGAAAGCATTGACCGTCGCCAGCGCTATCATAACGTACGCCGAGAAATGGCTCCGAGATCTTGTCCGAGACCACTCCGGAACCGCCGCGAAATTGGCCGCTTCTGACGACTCCGATTTGCGGCTTCAACAACGGGAGCTTCTGGAATCGATCGTCGAGATCTTACCGACTGAGAAAGCCGTTCTCCCGATTAACTTTCTCTGCTGCCTCCTCAGATCTGCGATCTTCGTCAAGGCGTCGAGCACGTGCAAGACCGAGCTCGAGAGGCGGATCTCGGCGGTCCTGGAGCACGTGACAGTCGACGACCTCCTGGTCCTGTCGTTCACCTACGACGGCGAGAGGCTCTTCGATCTGGAGAGCGTGAGGAGGATTATCTCCGGGTTCGTGGAGAAGGAGAAGAGCGTCGCGGTCTTCAACGCCGGCGATTTCAGGGAGGTTTGCTCGGCGGCGATGATTAGAATCGCAAAGACTGTCGACGCGTATCTTGGTGAGATCGCCACGTGTGTTGGGCTCAGTATCTCGAAGTTCAATGGCATCGCCAGCCTCATCCCCAAAGGCGCGCGCAAAGTCGACGACGATCTCTATCGTGCTATTGATATTTACTTGAAGTCTCACCCGAACCTTGATGAGATAGAGAAGGAGAAGGTGTGCAGCGTAATGGACGCATTGAAGTTGTCTTACGAAGCGAGAGTGCACGCTTCGCAAAACAAACGACTTCCCGTTCAAATCGTACTACACGCGTTGTATTACGACCAACTAAAGCTGAGGAGCGGGGTGGATGAAAACGACGTGCAAGACGCAATAACGACGAGGAGTCAGGTACAACACGACGTGTCGTTGGCGAGGGAGAACGAGGAGTTGAGAACAGAGTTGCTGAAGATGAAGATGTACATTACGGACCTGCATAAGACTGGGCCTGCGGCTGTTGCGACGACGTCGTCTTGTAAAGGAGGTGGCGGGCCCAAAAAGGCGAAATTTTTCTCGTATGTATCGAAGAAATTGGGGAGGTTAAATCCCTTCAAGCAAGGGTCGAAGGACACTTCGAACATCGTGGATGAGGATGTGGATATTACCAAGCCTAGAAGACGAAGGTTTTCCATCTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

578

Amino Acids

64.23

Weight (kDa)

7.5

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	26 - 119	4.5e-08	BTB/POZ domain
NPH3	PF03000	184 - 438	1.8e-78	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005865)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30520 AT2G30520 AT2G30520
fragaria_vesca	FvH4_3g40860 FvH4_3g40860 FvH4_3g40860 FvH4_3g40860
malus_domestica	MD03G1048900.v1.1 MD11G1051400.v1.1
prunus_persica	Prupe.6G039200_v2.0.a1 Prupe.6G039200_v2.0.a1
pyrus_communis	pycom03g03840 pycom11g04120
rosa_chinensis	RchiOBHm_Chr5g0073591
rosa_laevigata	RLG00000036398
rosa_multiflora	Rmu_co8263269.1_g000001 Rmu_co8430755.1_g000001 Rmu_sc0042142.1_g000002 Rmu_ssc0000242.1_g000005
rosa_roxburghii	Rroxscaffold_1G00007670
rosa_rugosa	Rorug05G0425200 Rorug05G0425300 Rorug05G0425400
rosa_samantha	Rh5AG482200 Rh5BG503000 Rh5CG526100 Rh5DG515200
rosa_wichuraiana	Rw5G044800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	938, 1564
AatII	GACGTC	1 cut(s)	1565
Acc36I	ACCTGC	2 cut(s)	446, 1536
AccB1I	GGYRCC	1 cut(s)	416
AccBSI	CCGCTC	1 cut(s)	1398
AccI	GTMKAC	3 cut(s)	927, 1101, 1197
AccII	CGCG	8 cut(s)	317, 624, 716, 1034, 1106, 1187, 1189, 1367
AccIII	TCCGGA	1 cut(s)	704
AclWI	GGATC	1 cut(s)	905
AcoI	YGGCCR	5 cut(s)	55, 144, 252, 367, 723
AcsI	RAATTY	2 cut(s)	527, 1601
AcuI	CTGAAG	1 cut(s)	1523
AcvI	CACGTG	3 cut(s)	873, 919, 1128
AcyI	GRCGYC	4 cut(s)	270, 417, 863, 1562
AdeI	CACNNNGTG	1 cut(s)	1459
AfaI	GTAC	6 cut(s)	347, 480, 665, 1359, 1449, 1517
AfeI	AGCGCT	1 cut(s)	652
AfiI	CCNNNNNNNGG	5 cut(s)	43, 316, 457, 675, 747
AflIII	ACRYGT	4 cut(s)	1104, 1127, 1365, 1458
AgsI	TTSAA	9 cut(s)	101, 616, 755, 1042, 1156, 1234, 1297, 1352, 1648
AhdI	GACNNNNNGTC	1 cut(s)	1440
AjiI	CACGTC	2 cut(s)	1417, 1459
AjnI	CCWGG	2 cut(s)	909, 936
AloI	GAACNNNNNNTCC	2 cut(s)	572, 604
AluBI	AGCT	5 cut(s)	389, 619, 766, 886, 1390
AluI	AGCT	5 cut(s)	389, 619, 766, 886, 1390
Alw21I	GWGCWC	5 cut(s)	432, 872, 888, 918, 1323
Alw26I	GTCTC	3 cut(s)	230, 690, 1242
Alw44I	GTGCAC	1 cut(s)	1319
AlwI	GGATC	1 cut(s)	905
Ama87I	CYCGRG	2 cut(s)	233, 887
Aor13HI	TCCGGA	1 cut(s)	704
Aor51HI	AGCGCT	1 cut(s)	652
AoxI	GGCC	8 cut(s)	55, 144, 229, 252, 367, 723, 1541, 1586
ApaI	GGGCCC	1 cut(s)	1590
ApaLI	GTGCAC	1 cut(s)	1319
ApeKI	GCWGC	4 cut(s)	312, 619, 831, 1278
ApoI	RAATTY	2 cut(s)	527, 1601
Asp700I	GAANNNNTTC	2 cut(s)	1604, 1720
AspLEI	GCGC	8 cut(s)	51, 317, 419, 624, 653, 1187, 1189, 1191
AspS9I	GGNCC	9 cut(s)	193, 230, 466, 907, 940, 1525, 1541, 1586, 1587
AsuC2I	CCSGG	2 cut(s)	225, 1006
AsuHPI	GGTGA	5 cut(s)	119, 355, 943, 1127, 1232
AsuII	TTCGAA	1 cut(s)	1670
AvaI	CYCGRG	2 cut(s)	233, 887
AvaII	GGWCC	5 cut(s)	193, 466, 907, 940, 1525
BaeGI	GKGCMC	2 cut(s)	1323, 1590
BalI	TGGCCA	2 cut(s)	57, 146
BanI	GGYRCC	1 cut(s)	416
BanII	GRGCYC	3 cut(s)	888, 1140, 1590
BarI	GAAGNNNNNNTAC	2 cut(s)	1289, 1321
BauI	CACGAG	2 cut(s)	140, 403
BbrPI	CACGTG	3 cut(s)	873, 919, 1128
BbsI	GAAGAC	3 cut(s)	450, 1030, 1717
Bbv12I	GWGCWC	5 cut(s)	432, 872, 888, 918, 1323
BbvCI	CCTCAGC	1 cut(s)	1391
BbvI	GCAGC	4 cut(s)	299, 606, 818, 1290
BccI	CCATC	1 cut(s)	1733
BceAI	ACGGC	3 cut(s)	283, 791, 976
BcgI	CGANNNNNNTGC	2 cut(s)	586, 620
BciT130I	CCWGG	2 cut(s)	911, 938
BcnI	CCSGG	2 cut(s)	225, 1006
BcoDI	GTCTC	3 cut(s)	230, 690, 1242
BfaI	CTAG	1 cut(s)	1707
BfoI	RGCGCY	2 cut(s)	420, 654
BfuAI	ACCTGC	2 cut(s)	446, 1536
BglI	GCCNNNNNGGC	1 cut(s)	414
BglII	AGATCT	3 cut(s)	685, 786, 842
BmcAI	AGTACT	1 cut(s)	347
Bme1390I	CCNGG	4 cut(s)	225, 911, 938, 1006
Bme18I	GGWCC	5 cut(s)	193, 466, 907, 940, 1525
BmeRI	GACNNNNNGTC	1 cut(s)	1440
BmeT110I	CYCGRG	2 cut(s)	233, 887
BmgBI	CACGTC	2 cut(s)	1417, 1459
BmgT120I	GGNCC	9 cut(s)	193, 230, 466, 907, 940, 1525, 1541, 1586, 1587
BmiI	GGNNCC	5 cut(s)	232, 418, 680, 709, 1588
BmrFI	CCNGG	4 cut(s)	225, 911, 938, 1006
BmrI	ACTGGG	1 cut(s)	1548
BmsI	GCATC	2 cut(s)	476, 1170
BmuI	ACTGGG	1 cut(s)	1548
BpiI	GAAGAC	3 cut(s)	450, 1030, 1717
BplI	GAGNNNNNCTC	2 cut(s)	1052, 1084
BpmI	CTGGAG	2 cut(s)	932, 1001
Bpu10I	CCTNAGC	2 cut(s)	390, 1391
Bpu14I	TTCGAA	1 cut(s)	1670
BpuEI	CTTGAG	2 cut(s)	57, 416
BpuMI	CCSGG	2 cut(s)	225, 1006
Bsa29I	ATCGAT	1 cut(s)	776
BsaAI	YACGTR	3 cut(s)	873, 919, 1128
BsaBI	GATNNNNATC	2 cut(s)	1085, 1209
BsaHI	GRCGYC	4 cut(s)	270, 417, 863, 1562
BsaI	GGTCTC	1 cut(s)	690
BsaJI	CCNNGG	3 cut(s)	42, 456, 507
BsaWI	WCCGGW	2 cut(s)	190, 704
BsaXI	ACNNNNNCTCC	4 cut(s)	17, 47, 1259, 1289
Bsc4I	CCNNNNNNNGG	5 cut(s)	43, 316, 457, 675, 747
Bse118I	RCCGGY	2 cut(s)	365, 1046
Bse1I	ACTGG	2 cut(s)	58, 1543
Bse8I	GATNNNNATC	2 cut(s)	1085, 1209
BseAI	TCCGGA	1 cut(s)	704
BseBI	CCWGG	2 cut(s)	911, 938
BseCI	ATCGAT	1 cut(s)	776
BseDI	CCNNGG	3 cut(s)	42, 456, 507
BseGI	GGATG	4 cut(s)	1173, 1411, 1687, 1693
BseJI	GATNNNNATC	2 cut(s)	1085, 1209
BseLI	CCNNNNNNNGG	5 cut(s)	43, 316, 457, 675, 747
BseMII	CTCAG	7 cut(s)	28, 404, 552, 789, 853, 1153, 1382
BseNI	ACTGG	2 cut(s)	58, 1543
BsePI	GCGCGC	2 cut(s)	1185, 1187
BseRI	GAGGAG	5 cut(s)	827, 1006, 1408, 1453, 1496
BseSI	GKGCMC	2 cut(s)	1323, 1590
BseX3I	CGGCCG	1 cut(s)	367
BseXI	GCAGC	4 cut(s)	299, 606, 818, 1290
BsgI	GTGCAG	1 cut(s)	1297
Bsh1236I	CGCG	8 cut(s)	317, 624, 716, 1034, 1106, 1187, 1189, 1367
Bsh1285I	CGRYCG	3 cut(s)	370, 542, 780
BshFI	GGCC	8 cut(s)	57, 146, 231, 254, 369, 725, 1543, 1588
BshNI	GGYRCC	1 cut(s)	416
BshVI	ATCGAT	1 cut(s)	776
BsiEI	CGRYCG	3 cut(s)	370, 542, 780
BsiHKAI	GWGCWC	5 cut(s)	432, 872, 888, 918, 1323
BsiHKCI	CYCGRG	2 cut(s)	233, 887
BsiSI	CCGG	7 cut(s)	191, 225, 366, 414, 705, 1005, 1047
BsiWI	CGTACG	1 cut(s)	663
BslI	CCNNNNNNNGG	5 cut(s)	43, 316, 457, 675, 747
BsmAI	GTCTC	3 cut(s)	230, 690, 1242
BsmBI	CGTCTC	1 cut(s)	230
BsnI	GGCC	8 cut(s)	57, 146, 231, 254, 369, 725, 1543, 1588
Bso31I	GGTCTC	1 cut(s)	690
BsoBI	CYCGRG	2 cut(s)	233, 887
Bsp119I	TTCGAA	1 cut(s)	1670
Bsp120I	GGGCCC	1 cut(s)	1586
Bsp1286I	GDGCHC	7 cut(s)	432, 872, 888, 918, 1140, 1323, 1590
Bsp13I	TCCGGA	1 cut(s)	704
Bsp1407I	TGTACA	2 cut(s)	478, 1515
Bsp19I	CCATGG	2 cut(s)	42, 456
BspANI	GGCC	8 cut(s)	57, 146, 231, 254, 369, 725, 1543, 1588
BspCNI	CTCAG	7 cut(s)	27, 403, 553, 790, 852, 1152, 1383
BspDI	ATCGAT	1 cut(s)	776
BspEI	TCCGGA	1 cut(s)	704
BspFNI	CGCG	8 cut(s)	317, 624, 716, 1034, 1106, 1187, 1189, 1367
BspLI	GGNNCC	5 cut(s)	232, 418, 680, 709, 1588
BspMI	ACCTGC	2 cut(s)	446, 1536
BspPI	GGATC	1 cut(s)	905
BspQI	GCTCTTC	2 cut(s)	977, 1019
BspT104I	TTCGAA	1 cut(s)	1670
BspT107I	GGYRCC	1 cut(s)	416
BspTNI	GGTCTC	1 cut(s)	690
BsrBI	CCGCTC	1 cut(s)	1398
BsrFI	RCCGGY	2 cut(s)	365, 1046
BsrGI	TGTACA	2 cut(s)	478, 1515
BsrI	ACTGG	2 cut(s)	58, 1543
BssAI	RCCGGY	2 cut(s)	365, 1046
BssECI	CCNNGG	3 cut(s)	42, 456, 507
BssHII	GCGCGC	2 cut(s)	1185, 1187
BssNI	GRCGYC	4 cut(s)	270, 417, 863, 1562
BssSI	CACGAG	2 cut(s)	140, 403
BssT1I	CCWWGG	3 cut(s)	42, 456, 507
Bst2BI	CACGAG	2 cut(s)	140, 403
Bst2UI	CCWGG	2 cut(s)	911, 938
Bst4CI	ACNGT	4 cut(s)	289, 643, 925, 1099
Bst6I	CTCTTC	2 cut(s)	977, 1019
BstACI	GRCGYC	4 cut(s)	270, 417, 863, 1562
BstAUI	TGTACA	2 cut(s)	478, 1515
BstBAI	YACGTR	3 cut(s)	873, 919, 1128
BstBI	TTCGAA	1 cut(s)	1670
BstC8I	GCNNGC	8 cut(s)	649, 1048, 1168, 1187, 1189, 1323, 1545, 1586
BstDEI	CTNAG	8 cut(s)	14, 385, 390, 561, 798, 839, 1139, 1391
BstDSI	CCRYGG	2 cut(s)	42, 456
BstF5I	GGATG	4 cut(s)	1173, 1411, 1687, 1693
BstFNI	CGCG	8 cut(s)	317, 624, 716, 1034, 1106, 1187, 1189, 1367
BstH2I	RGCGCY	2 cut(s)	420, 654
BstHHI	GCGC	8 cut(s)	51, 317, 419, 624, 653, 1187, 1189, 1191
BstMAI	GTCTC	3 cut(s)	230, 690, 1242
BstMCI	CGRYCG	3 cut(s)	370, 542, 780
BstMWI	GCNNNNNNNGC	7 cut(s)	395, 414, 722, 892, 1167, 1327, 1553
BstNI	CCWGG	2 cut(s)	911, 938
BstSCI	CCNGG	4 cut(s)	223, 909, 936, 1004
BstSLI	GKGCMC	2 cut(s)	1323, 1590
BstUI	CGCG	8 cut(s)	317, 624, 716, 1034, 1106, 1187, 1189, 1367
BstV1I	GCAGC	4 cut(s)	299, 606, 818, 1290
BstV2I	GAAGAC	3 cut(s)	450, 1030, 1717
BstX2I	RGATCY	4 cut(s)	685, 786, 842, 897
BstYI	RGATCY	4 cut(s)	685, 786, 842, 897
BstZI	CGGCCG	1 cut(s)	367
Bsu15I	ATCGAT	1 cut(s)	776
BsuRI	GGCC	8 cut(s)	57, 146, 231, 254, 369, 725, 1543, 1588
BsuTUI	ATCGAT	1 cut(s)	776
BtgI	CCRYGG	2 cut(s)	42, 456
BtgZI	GCGATG	2 cut(s)	1091, 1147
BtrI	CACGTC	2 cut(s)	1417, 1459
BtsCI	GGATG	4 cut(s)	1173, 1411, 1687, 1693
BtsIMutI	CAGTG	1 cut(s)	51
BveI	ACCTGC	2 cut(s)	446, 1536
Cac8I	GCNNGC	8 cut(s)	649, 1048, 1168, 1187, 1189, 1323, 1545, 1586
CfoI	GCGC	8 cut(s)	51, 317, 419, 624, 653, 1187, 1189, 1191
Cfr10I	RCCGGY	2 cut(s)	365, 1046
Cfr13I	GGNCC	9 cut(s)	193, 230, 466, 907, 940, 1525, 1541, 1586, 1587
ClaI	ATCGAT	1 cut(s)	776
CseI	GACGC	7 cut(s)	259, 478, 852, 1018, 1112, 1298, 1433
Csp6I	GTAC	6 cut(s)	346, 479, 664, 1358, 1448, 1516
CspCI	CAANNNNNGTGG	2 cut(s)	1114, 1149
CviAII	CATG	3 cut(s)	43, 136, 457
CviQI	GTAC	6 cut(s)	346, 479, 664, 1358, 1448, 1516
DdeI	CTNAG	8 cut(s)	14, 385, 390, 561, 798, 839, 1139, 1391
DinI	GGCGCC	1 cut(s)	418
DraIII	CACNNNGTG	1 cut(s)	1459
DrdI	GACNNNNNNGTC	2 cut(s)	938, 1564
DriI	GACNNNNNGTC	1 cut(s)	1440
DseDI	GACNNNNNNGTC	2 cut(s)	938, 1564
EaeI	YGGCCR	5 cut(s)	55, 144, 252, 367, 723
EagI	CGGCCG	1 cut(s)	367
Eam1104I	CTCTTC	2 cut(s)	977, 1019
Eam1105I	GACNNNNNGTC	1 cut(s)	1440
EarI	CTCTTC	2 cut(s)	977, 1019
EciI	GGCGGA	3 cut(s)	335, 476, 910
Ecl136II	GAGCTC	1 cut(s)	886
EclXI	CGGCCG	1 cut(s)	367
Eco130I	CCWWGG	3 cut(s)	42, 456, 507
Eco24I	GRGCYC	3 cut(s)	888, 1140, 1590
Eco31I	GGTCTC	1 cut(s)	690
Eco47I	GGWCC	5 cut(s)	193, 466, 907, 940, 1525
Eco47III	AGCGCT	1 cut(s)	652
Eco52I	CGGCCG	1 cut(s)	367
Eco53kI	GAGCTC	1 cut(s)	886
Eco57I	CTGAAG	1 cut(s)	1523
Eco72I	CACGTG	3 cut(s)	873, 919, 1128
Eco88I	CYCGRG	2 cut(s)	233, 887
EcoICRI	GAGCTC	1 cut(s)	886
EcoO109I	RGGNCCY	2 cut(s)	230, 466
EcoRII	CCWGG	2 cut(s)	909, 936
EcoT14I	CCWWGG	3 cut(s)	42, 456, 507
EcoT38I	GRGCYC	3 cut(s)	888, 1140, 1590
EgeI	GGCGCC	1 cut(s)	418
EheI	GGCGCC	1 cut(s)	418
ErhI	CCWWGG	3 cut(s)	42, 456, 507
Esp3I	CGTCTC	1 cut(s)	230
FaeI	CATG	3 cut(s)	46, 139, 460
FaiI	YATR	7 cut(s)	44, 137, 161, 458, 659, 1533, 1614
FalI	AAGNNNNNCTT	2 cut(s)	435, 467
FatI	CATG	3 cut(s)	42, 135, 456
FauI	CCCGC	2 cut(s)	1391, 1577
FblI	GTMKAC	3 cut(s)	927, 1101, 1197
FokI	GGATG	4 cut(s)	1160, 1418, 1694, 1700
FriOI	GRGCYC	3 cut(s)	888, 1140, 1590
FspBI	CTAG	1 cut(s)	1707
GlaI	GCGC	8 cut(s)	50, 316, 418, 623, 652, 1186, 1188, 1190
GsuI	CTGGAG	2 cut(s)	932, 1001
HaeII	RGCGCY	2 cut(s)	420, 654
HaeIII	GGCC	8 cut(s)	57, 146, 231, 254, 369, 725, 1543, 1588
HapII	CCGG	7 cut(s)	191, 225, 366, 414, 705, 1005, 1047
HgaI	GACGC	7 cut(s)	259, 478, 852, 1018, 1112, 1298, 1433
HhaI	GCGC	8 cut(s)	51, 317, 419, 624, 653, 1187, 1189, 1191
Hin1I	GRCGYC	4 cut(s)	270, 417, 863, 1562
Hin1II	CATG	3 cut(s)	46, 139, 460
Hin6I	GCGC	8 cut(s)	49, 315, 417, 622, 651, 1185, 1187, 1189
HinP1I	GCGC	8 cut(s)	49, 315, 417, 622, 651, 1185, 1187, 1189
HincII	GTYRAC	4 cut(s)	274, 928, 1102, 1198
HindII	GTYRAC	4 cut(s)	274, 928, 1102, 1198
HinfI	GANTC	7 cut(s)	179, 204, 535, 737, 773, 1086, 1441
HpaII	CCGG	7 cut(s)	191, 225, 366, 414, 705, 1005, 1047
HphI	GGTGA	5 cut(s)	119, 355, 943, 1127, 1232
Hpy166II	GTNNAC	8 cut(s)	274, 292, 558, 928, 951, 1102, 1198, 1321
Hpy188I	TCNGA	8 cut(s)	88, 217, 534, 683, 695, 733, 742, 842
Hpy8I	GTNNAC	8 cut(s)	274, 292, 558, 928, 951, 1102, 1198, 1321
HpyAV	CCTTC	8 cut(s)	241, 461, 1012, 1258, 1264, 1654, 1654, 1710
HpyCH4III	ACNGT	4 cut(s)	289, 643, 925, 1099
HpyCH4IV	ACGT	9 cut(s)	239, 297, 662, 872, 918, 1127, 1416, 1458, 1562
HpyCH4V	TGCA	5 cut(s)	876, 1278, 1321, 1420, 1531
HpyF10VI	GCNNNNNNNGC	7 cut(s)	395, 414, 722, 892, 1167, 1327, 1553
HpyF3I	CTNAG	8 cut(s)	14, 385, 390, 561, 798, 839, 1139, 1391
HpySE526I	ACGT	9 cut(s)	239, 297, 662, 872, 918, 1127, 1416, 1458, 1562
Hsp92I	GRCGYC	4 cut(s)	270, 417, 863, 1562
Hsp92II	CATG	3 cut(s)	46, 139, 460
HspAI	GCGC	8 cut(s)	49, 315, 417, 622, 651, 1185, 1187, 1189
KasI	GGCGCC	1 cut(s)	416
Kpn2I	TCCGGA	1 cut(s)	704
KroI	GCCGGC	1 cut(s)	1046
KroNI	GCCGGC	1 cut(s)	1048
LguI	GCTCTTC	2 cut(s)	977, 1019
LmnI	GCTCC	5 cut(s)	46, 684, 763, 913, 1395
Lsp1109I	GCAGC	4 cut(s)	299, 606, 818, 1290
LweI	GCATC	2 cut(s)	476, 1170
MaeI	CTAG	1 cut(s)	1707
MaeII	ACGT	9 cut(s)	239, 297, 662, 872, 918, 1127, 1416, 1458, 1562
MaeIII	GTNAC	3 cut(s)	23, 263, 919
MbiI	CCGCTC	1 cut(s)	1398
MflI	RGATCY	4 cut(s)	685, 786, 842, 897
MhlI	GDGCHC	7 cut(s)	432, 872, 888, 918, 1140, 1323, 1590
MlsI	TGGCCA	2 cut(s)	57, 146
MluCI	AATT	8 cut(s)	115, 474, 527, 550, 566, 719, 1601, 1625
MluI	ACGCGT	2 cut(s)	1104, 1365
MluNI	TGGCCA	2 cut(s)	57, 146
Mly113I	GGCGCC	1 cut(s)	417
MlyI	GAGTC	4 cut(s)	188, 544, 731, 1450
MmeI	TCCRAC	1 cut(s)	240
Mox20I	TGGCCA	2 cut(s)	57, 146
MreI	CGCCGGCG	1 cut(s)	1046
MroI	TCCGGA	1 cut(s)	704
MroNI	GCCGGC	1 cut(s)	1046
MroXI	GAANNNNTTC	2 cut(s)	1604, 1720
MscI	TGGCCA	2 cut(s)	57, 146
MseI	TTAA	3 cut(s)	819, 1637, 1732
MslI	CAYNNNNRTG	2 cut(s)	140, 425
Msp20I	TGGCCA	2 cut(s)	57, 146
MspA1I	CMGCKG	2 cut(s)	18, 312
MspI	CCGG	7 cut(s)	191, 225, 366, 414, 705, 1005, 1047
MspR9I	CCNGG	4 cut(s)	225, 911, 938, 1006
MvaI	CCWGG	2 cut(s)	911, 938
MvnI	CGCG	8 cut(s)	317, 624, 716, 1034, 1106, 1187, 1189, 1367
MwoI	GCNNNNNNNGC	7 cut(s)	395, 414, 722, 892, 1167, 1327, 1553
NaeI	GCCGGC	1 cut(s)	1048
NarI	GGCGCC	1 cut(s)	417
NciI	CCSGG	2 cut(s)	225, 1006
NcoI	CCATGG	2 cut(s)	42, 456
NgoMIV	GCCGGC	1 cut(s)	1046
NlaIII	CATG	3 cut(s)	46, 139, 460
NlaIV	GGNNCC	5 cut(s)	232, 418, 680, 709, 1588
NmeAIII	GCCGAG	3 cut(s)	694, 881, 1049
NmuCI	GTSAC	1 cut(s)	919
NspV	TTCGAA	1 cut(s)	1670
PaeR7I	CTCGAG	1 cut(s)	887
PauI	GCGCGC	2 cut(s)	1185, 1187
PciSI	GCTCTTC	2 cut(s)	977, 1019
PcsI	WCGNNNNNNNCGW	2 cut(s)	924, 1616
PdiI	GCCGGC	1 cut(s)	1048
PdmI	GAANNNNTTC	2 cut(s)	1604, 1720
PfeI	GAWTC	3 cut(s)	204, 773, 1086
Pfl23II	CGTACG	1 cut(s)	663
PflFI	GACNNNGTC	2 cut(s)	1561, 1564
PfoI	TCCNGGA	2 cut(s)	223, 909
Ple19I	CGATCG	2 cut(s)	542, 780
PleI	GAGTC	4 cut(s)	187, 543, 731, 1449
PluTI	GGCGCC	1 cut(s)	420
PmaCI	CACGTG	3 cut(s)	873, 919, 1128
PmlI	CACGTG	3 cut(s)	873, 919, 1128
PpsI	GAGTC	4 cut(s)	187, 543, 731, 1449
Ppu21I	YACGTR	3 cut(s)	873, 919, 1128
PpuMI	RGGWCCY	1 cut(s)	466
Psp124BI	GAGCTC	1 cut(s)	888
Psp5II	RGGWCCY	1 cut(s)	466
Psp6I	CCWGG	2 cut(s)	909, 936
PspCI	CACGTG	3 cut(s)	873, 919, 1128
PspGI	CCWGG	2 cut(s)	909, 936
PspLI	CGTACG	1 cut(s)	663
PspN4I	GGNNCC	5 cut(s)	232, 418, 680, 709, 1588
PspOMI	GGGCCC	1 cut(s)	1586
PspPI	GGNCC	9 cut(s)	193, 230, 466, 907, 940, 1525, 1541, 1586, 1587
PspPPI	RGGWCCY	1 cut(s)	466
PsuI	RGATCY	4 cut(s)	685, 786, 842, 897
PsyI	GACNNNGTC	2 cut(s)	1561, 1564
PteI	GCGCGC	2 cut(s)	1185, 1187
PvuI	CGATCG	2 cut(s)	542, 780
RsaI	GTAC	6 cut(s)	347, 480, 665, 1359, 1449, 1517
RsaNI	GTAC	6 cut(s)	346, 479, 664, 1358, 1448, 1516
RseI	CAYNNNNRTG	2 cut(s)	140, 425
SacI	GAGCTC	1 cut(s)	888
SalI	GTCGAC	3 cut(s)	926, 1100, 1196
SapI	GCTCTTC	2 cut(s)	977, 1019
SaqAI	TTAA	3 cut(s)	819, 1637, 1732
Sau96I	GGNCC	9 cut(s)	193, 230, 466, 907, 940, 1525, 1541, 1586, 1587
ScaI	AGTACT	1 cut(s)	347
SchI	GAGTC	4 cut(s)	188, 544, 731, 1450
ScrFI	CCNGG	4 cut(s)	225, 911, 938, 1006
SduI	GDGCHC	7 cut(s)	432, 872, 888, 918, 1140, 1323, 1590
SfaNI	GCATC	2 cut(s)	476, 1170
SfoI	GGCGCC	1 cut(s)	418
Sfr274I	CTCGAG	1 cut(s)	887
SfuI	TTCGAA	1 cut(s)	1670
SgrAI	CRCCGGYG	1 cut(s)	1046
SinI	GGWCC	5 cut(s)	193, 466, 907, 940, 1525
SlaI	CTCGAG	1 cut(s)	887
SmiMI	CAYNNNNRTG	2 cut(s)	140, 425
SmlI	CTYRAG	3 cut(s)	72, 431, 887
SmoI	CTYRAG	3 cut(s)	72, 431, 887
Sse9I	AATT	8 cut(s)	115, 474, 527, 550, 566, 719, 1601, 1625
SspDI	GGCGCC	1 cut(s)	416
SspMI	CTAG	1 cut(s)	1707
SstI	GAGCTC	1 cut(s)	888
StyD4I	CCNGG	4 cut(s)	223, 909, 936, 1004
StyI	CCWWGG	3 cut(s)	42, 456, 507
TaaI	ACNGT	4 cut(s)	289, 643, 925, 1099
TaiI	ACGT	9 cut(s)	242, 300, 665, 875, 921, 1130, 1419, 1461, 1565
TaqII	GACCGA	1 cut(s)	896
TasI	AATT	8 cut(s)	115, 474, 527, 550, 566, 719, 1601, 1625
TatI	WGTACW	3 cut(s)	345, 478, 1515
TfiI	GAWTC	3 cut(s)	204, 773, 1086
Tru1I	TTAA	3 cut(s)	819, 1637, 1732
Tru9I	TTAA	3 cut(s)	819, 1637, 1732
TscAI	CASTG	1 cut(s)	58
TseFI	GTSAC	1 cut(s)	919
TseI	GCWGC	4 cut(s)	312, 619, 831, 1278
Tsp45I	GTSAC	1 cut(s)	919
TspDTI	ATGAA	3 cut(s)	124, 1422, 1523
TspGWI	ACGGA	2 cut(s)	72, 1538
TspRI	CASTG	1 cut(s)	58
Tth111I	GACNNNGTC	2 cut(s)	1561, 1564
VneI	GTGCAC	1 cut(s)	1319
VpaK11BI	GGWCC	5 cut(s)	193, 466, 907, 940, 1525
XapI	RAATTY	2 cut(s)	527, 1601
XhoI	CTCGAG	1 cut(s)	887
XmiI	GTMKAC	3 cut(s)	927, 1101, 1197
XmnI	GAANNNNTTC	2 cut(s)	1604, 1720
XspI	CTAG	1 cut(s)	1707
ZraI	GACGTC	1 cut(s)	1563
ZrmI	AGTACT	1 cut(s)	347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.