Rorug05G0425200

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005865
Physical Location & Seq
Forward (+)
59060117 .. 59061147
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0425200.1

Sequence Viewer

Length: 672 bp
ATGAGAAGCTTTGGAAAAACCGATCTCAAACTAGCTCGGGGTTGGTGGCTTCAGCTATGGCATGGTGACACATTGAAATTGAATGTGGATGGTGCTTTTTTACCTAATGTGCAATTTGGGGGTACAAGAGGTGTACTTCGTGATGCTTATGGTAACTTCCTTGCTGCTTTCTCTAGTCGTATGAATTTTGTTAGTTCTCCGTTACACGTTGAACTCTTGGCTATGAAGAAGGGTATGGAGTTGCTGCAAGCCATGCAGATCAGGAACGCAGTAATAGAAAGTGATTGTCTAATGGCTGTACAAGCCATCAACGCTTTGGATTGTGATCTGTCACCAGTAAGAGCCTTAGTTGCTGATGTTCGAAATCTCCTCAATTCGAGTGTTGAGTTATATATTACTTTTGCTCCTAGACAGGCTAATATTGCAGCTCATAGATTAGCTACTCTTAGTTTTGAGTCTGAGATACAATTTGAATGGTTTGTAACTGCTCCAGACTTTATACTAGATGCCCTCATAATTGTTCTAGTCTGGTGGCACCCGGAAGTCTTGATTGGCCTCCGGCCTCGCCGCCGTCTTGGGGTTTGCTGCCGGACGGGAGATCTACTGCCCATGGGACTTCGTCTAAGGGATTCTCTCGGATTTGTCAGATGGTTTGGCTGGGATGGTGGCTAA

Protein Analysis

223

Amino Acids

25.12

Weight (kDa)

8.44

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 28 - 148 1.9e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 534
AciI CCGC 1 cut(s) 568
AcsI RAATTY 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 3 cut(s) 124, 135, 300
AfiI CCNNNNNNNGG 1 cut(s) 577
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 4 cut(s) 76, 82, 212, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 534, 566
AluBI AGCT 5 cut(s) 9, 35, 55, 428, 440
AluI AGCT 5 cut(s) 9, 35, 55, 428, 440
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 2 cut(s) 553, 560
ApeKI GCWGC 4 cut(s) 164, 244, 425, 585
ApoI RAATTY 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 2 cut(s) 77, 324
AsuII TTCGAA 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 36
BanI GGYRCC 1 cut(s) 534
BbvI GCAGC 4 cut(s) 151, 231, 437, 572
BccI CCATC 4 cut(s) 83, 314, 642, 656
BceAI ACGGC 1 cut(s) 555
BcnI CCSGG 1 cut(s) 539
BfaI CTAG 5 cut(s) 32, 174, 408, 503, 524
BglII AGATCT 1 cut(s) 598
BisI GCNGC 5 cut(s) 165, 245, 426, 568, 586
BlsI GCNGC 5 cut(s) 166, 246, 427, 569, 587
Bme1390I CCNGG 1 cut(s) 539
BmeT110I CYCGRG 1 cut(s) 36
BmiI GGNNCC 1 cut(s) 536
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 2 cut(s) 133, 496
BpmI CTGGAG 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 361
BpuMI CCSGG 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 609
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bsc4I CCNNNNNNNGG 1 cut(s) 577
Bse1I ACTGG 1 cut(s) 335
Bse8I GATNNNNATC 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 609
BseGI GGATG 2 cut(s) 94, 667
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 577
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 4 cut(s) 151, 231, 437, 572
BseYI CCCAGC 1 cut(s) 657
BshFI GGCC 2 cut(s) 555, 562
BshNI GGYRCC 1 cut(s) 534
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 3 cut(s) 539, 559, 589
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 1 cut(s) 577
BsmFI GGGAC 1 cut(s) 627
BsnI GGCC 2 cut(s) 555, 562
BsoBI CYCGRG 1 cut(s) 36
Bsp119I TTCGAA 1 cut(s) 361
Bsp1407I TGTACA 1 cut(s) 298
Bsp143I GATC 4 cut(s) 22, 258, 325, 598
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 1 cut(s) 568
BspANI GGCC 2 cut(s) 555, 562
BspCNI CTCAG 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 536
BspT104I TTCGAA 1 cut(s) 361
BspT107I GGYRCC 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 298
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 609
BssMI GATC 4 cut(s) 22, 258, 325, 598
BssT1I CCWWGG 1 cut(s) 609
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 298
BstBI TTCGAA 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 4 cut(s) 346, 446, 459, 623
BstDSI CCRYGG 1 cut(s) 609
BstF5I GGATG 2 cut(s) 94, 667
BstKTI GATC 4 cut(s) 25, 261, 328, 601
BstMBI GATC 4 cut(s) 22, 258, 325, 598
BstMWI GCNNNNNNNGC 5 cut(s) 253, 302, 311, 350, 422
BstSCI CCNGG 1 cut(s) 537
BstV1I GCAGC 4 cut(s) 151, 231, 437, 572
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 2 cut(s) 555, 562
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 2 cut(s) 94, 667
Cac8I GCNNGC 1 cut(s) 249
Csp6I GTAC 3 cut(s) 123, 134, 299
CviAII CATG 3 cut(s) 62, 253, 610
CviQI GTAC 3 cut(s) 123, 134, 299
DdeI CTNAG 4 cut(s) 346, 446, 459, 623
DpnI GATC 4 cut(s) 24, 260, 327, 600
DpnII GATC 4 cut(s) 22, 258, 325, 598
Eco130I CCWWGG 1 cut(s) 609
Eco57I CTGAAG 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 3 cut(s) 65, 256, 613
FaqI GGGAC 1 cut(s) 627
FatI CATG 3 cut(s) 61, 252, 609
Fnu4HI GCNGC 5 cut(s) 165, 245, 426, 568, 586
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 5 cut(s) 165, 245, 426, 568, 586
FspBI CTAG 5 cut(s) 32, 174, 408, 503, 524
GluI GCNGC 5 cut(s) 165, 245, 426, 568, 586
GsaI CCCAGC 1 cut(s) 661
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 555, 562
HapII CCGG 3 cut(s) 539, 559, 589
Hin1II CATG 3 cut(s) 65, 256, 613
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 2 cut(s) 455, 629
HpaII CCGG 3 cut(s) 539, 559, 589
HphI GGTGA 2 cut(s) 77, 324
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 3 cut(s) 460, 638, 647
Hpy188III TCNNGA 4 cut(s) 140, 262, 491, 547
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 4 cut(s) 112, 247, 256, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 253, 302, 311, 350, 422
HpyF3I CTNAG 4 cut(s) 346, 446, 459, 623
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 3 cut(s) 65, 256, 613
Kzo9I GATC 4 cut(s) 22, 258, 325, 598
LmnI GCTCC 2 cut(s) 409, 493
LpnPI CCDG 9 cut(s) 247, 348, 398, 504, 514, 552, 572, 602, 643
Lsp1109I GCAGC 4 cut(s) 151, 231, 437, 572
LweI GCATC 2 cut(s) 133, 496
MaeI CTAG 5 cut(s) 32, 174, 408, 503, 524
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 5 cut(s) 65, 152, 201, 330, 481
MalI GATC 4 cut(s) 24, 260, 327, 600
MboI GATC 4 cut(s) 22, 258, 325, 598
MboII GAAGA 1 cut(s) 238
MflI RGATCY 1 cut(s) 598
MluCI AATT 6 cut(s) 77, 113, 184, 373, 467, 516
MlyI GAGTC 1 cut(s) 464
MnlI CCTC 5 cut(s) 122, 380, 521, 566, 573
MspI CCGG 3 cut(s) 539, 559, 589
MspR9I CCNGG 1 cut(s) 539
MwoI GCNNNNNNNGC 5 cut(s) 253, 302, 311, 350, 422
NciI CCSGG 1 cut(s) 539
NcoI CCATGG 1 cut(s) 609
NdeII GATC 4 cut(s) 22, 258, 325, 598
NlaIII CATG 3 cut(s) 65, 256, 613
NlaIV GGNNCC 1 cut(s) 536
NmuCI GTSAC 2 cut(s) 65, 330
NspV TTCGAA 1 cut(s) 361
PfeI GAWTC 1 cut(s) 629
PflFI GACNNNGTC 1 cut(s) 618
PkrI GCNGC 5 cut(s) 166, 246, 427, 569, 587
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 657
PspN4I GGNNCC 1 cut(s) 536
PsuI RGATCY 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 618
RsaI GTAC 3 cut(s) 124, 135, 300
RsaNI GTAC 3 cut(s) 123, 134, 299
SatI GCNGC 5 cut(s) 165, 245, 426, 568, 586
Sau3AI GATC 4 cut(s) 22, 258, 325, 598
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 1 cut(s) 539
SetI ASST 8 cut(s) 11, 37, 57, 106, 133, 210, 430, 442
SfaNI GCATC 2 cut(s) 133, 496
SfuI TTCGAA 1 cut(s) 361
Sse9I AATT 6 cut(s) 77, 113, 184, 373, 467, 516
SsiI CCGC 1 cut(s) 568
SspI AATATT 1 cut(s) 421
SspMI CTAG 5 cut(s) 32, 174, 408, 503, 524
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 1 cut(s) 609
TaiI ACGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 361, 377
TasI AATT 6 cut(s) 77, 113, 184, 373, 467, 516
TatI WGTACW 2 cut(s) 133, 298
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 1 cut(s) 629
TseFI GTSAC 2 cut(s) 65, 330
TseI GCWGC 4 cut(s) 164, 244, 425, 585
Tsp45I GTSAC 2 cut(s) 65, 330
TspDTI ATGAA 2 cut(s) 197, 239
TspGWI ACGGA 1 cut(s) 189
Tth111I GACNNNGTC 1 cut(s) 618
XapI RAATTY 1 cut(s) 184
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XspI CTAG 5 cut(s) 32, 174, 408, 503, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.