Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0005865

Physical Location & Seq

Genomic Coordinates Forward (+)

3042005 .. 3045293

Gene Structure 3289 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g03840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGCATGAATCTATAATTATCTCAGGTCACATAACTCTCTCTCTCTTCTCTCTCTACACAAGAGCTCCAAGCTCCTCCCACCTCCTCCCACCTCCCATGGCTTCTCCTCTCAGTGGCGGCAACAGGCTCTCCCTTGCCATGGAGCGCACTGGCCAATGGATTATCTCTCAAGATATCCCGTCAGATGTTGTGGTTGAAGTTGGTGAAGCCAATTTCTCTCTACACAAGTTCATGCTCGTGGCCAAGAGCAACCGCATAAGAAAACTGATAATGGAGTCAAAACAATCGGACCTAACGAGAATCGACCTCTCTGACATTCCCGGAGGCCCCGAGACGTTCGAGAAGGCGGCCAAGTTCTGTTACGGCGTCAACTTTGAGATCACTGTCCACAACGTCGCCGCCCTCCGCTGCGCGGCGGAGTATTTGGAAATGACCGATAAGTACTGCAACAACAACCTCACCAGCCGCACTGAAGATTTCTTAACTCAGGTCGCTCTCACGAGCCTCTCCGGCGCCATTGTCGTGCTCAAGACCTGCGAAGACCTCCTTCCCATGGCGGAGGACCTCAAAATTGTACAGAGATGCGTCGATGTCGCCACTTCCAAGGTATCAATCGAAGCGAAATTTCCGAGTCGATCGCCGACGAATTGGTGGACTGAGGAATTGTCGATTTTGGACATTGAGTTCTTCGGAAGAGTAATCGCCTCGATGAAGCTGCGCTGCGTGAAATCATTGACCGTCGCCAGCGCTATCATAACGTACGCCGAGAAATGGCTCCGAGATCTCGTCCGAGACCACTCCGGAACGGCAGCGAAATTGGCCGCTTCTGACGACTCCGATTTGCGGCTCCAACAACGGGAGCTTCTGGAATCGATCATCGAGATCTTACCGACTGAGAAAGCCACTCTCCCGATTAACTTCCTCTGCTGCCTCCTCAGATCTACGATCTTCGTCAAGGCGTCGAGCACGTGCAAGACCGAGCTCGAGAGGCGGATCTCGGCGGTCCTGGAGCACGTGACCGTCGACGACCTCCTGGTCCTGTCGTTCACCTACGACGGCGAGAGGCTCTTCGATCTAGAGAGCGTGAGGAGGATTATCTCCGGGTTTATGGAGAAGGAGAAGAGCGTCGCGGTCTTCAACGCCGGCGATTTCAGGGAGGTTTGCTCGGCGGCGATGATCAGAGTCGCGAAGACTGTCGACGCGTATCTTGGTGAGATCGCCACTTGTGTTGGGCTCAGTATCTCTAAGTTCAACGGCATCGCCAGCCTCATCCCCAAAGGCGCGCGCAAAGTCGACGACGATCTCTATCGTGCTATTGATATTTACTTGAAGGCTCACCCGAACCTTGATGAGATAGAGAAGGAGAAGGTGTGCAGCGTAATGGACGCACTGAAGTTGTCGTACGAAGCGAGAGTGCACGCTTCGCAAAACAAACGACTTCCCGTTCAAATTGTACTACATGCATTGTATTACGACCAGCTAAAGCTGAGGAGTGGGGTGGAAGAAAACGACGTGCAAGACGCAACAATGACAAGGAGACAGGTGCAACACGACGTGTCGTTGGCGAGGGAAAACGAGGAGTTGAGAACGGAGTTGTTGAAGATGAAGATGTACATTACGGACCTGCAGAAGACTGGGCCTGCAGGTGTTGCGACGACGTCGTCGTGTAAAGGAGGTGGCGGGCCCAAAAAGGCGACATTTTTCTCGTCTGTGTCGAAGAAATTGGGGAGGTTAAATCCCTTCAAGCAAGGGTCGAAGGACACTATGAACATCGTGGATGAGGATGTAGATATTACCAAGCCTAGAAGAAGAAGGTTTTCCATCTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

610

Amino Acids

67.88

Weight (kDa)

7.14

Isoelectric Point (pI)

42.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	58 - 146	2.4e-08	BTB/POZ domain
NPH3	PF03000	216 - 470	5.2e-78	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005865)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30520 AT2G30520 AT2G30520
fragaria_vesca	FvH4_3g40860 FvH4_3g40860 FvH4_3g40860 FvH4_3g40860
malus_domestica	MD03G1048900.v1.1 MD11G1051400.v1.1
prunus_persica	Prupe.6G039200_v2.0.a1 Prupe.6G039200_v2.0.a1
pyrus_communis	pycom03g03840 pycom11g04120
rosa_chinensis	RchiOBHm_Chr5g0073591
rosa_laevigata	RLG00000036398
rosa_multiflora	Rmu_co8263269.1_g000001 Rmu_co8430755.1_g000001 Rmu_sc0042142.1_g000002 Rmu_ssc0000242.1_g000005
rosa_roxburghii	Rroxscaffold_1G00007670
rosa_rugosa	Rorug05G0425200 Rorug05G0425300 Rorug05G0425400
rosa_samantha	Rh5AG482200 Rh5BG503000 Rh5CG526100 Rh5DG515200
rosa_wichuraiana	Rw5G044800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1634
AasI	GACNNNNNNGTC	2 cut(s)	1034, 1660
AatII	GACGTC	1 cut(s)	1661
Acc36I	ACCTGC	3 cut(s)	542, 1632, 1634
AccB1I	GGYRCC	1 cut(s)	512
AccI	GTMKAC	3 cut(s)	1023, 1197, 1293
AccII	CGCG	6 cut(s)	413, 1130, 1187, 1202, 1283, 1285
AccIII	TCCGGA	1 cut(s)	800
AclWI	GGATC	1 cut(s)	1001
AcoI	YGGCCR	4 cut(s)	151, 240, 348, 819
AcsI	RAATTY	1 cut(s)	623
AcuI	CTGAAG	2 cut(s)	492, 1412
AcvI	CACGTG	2 cut(s)	969, 1015
AcyI	GRCGYC	4 cut(s)	366, 513, 959, 1658
AdeI	CACNNNGTG	1 cut(s)	1555
AfaI	GTAC	6 cut(s)	443, 576, 761, 1403, 1455, 1613
AfeI	AGCGCT	1 cut(s)	748
AfiI	CCNNNNNNNGG	7 cut(s)	113, 139, 412, 553, 771, 843, 856
AflIII	ACRYGT	2 cut(s)	1200, 1554
AgsI	TTSAA	7 cut(s)	197, 1138, 1252, 1330, 1448, 1600, 1744
AjiI	CACGTC	2 cut(s)	1513, 1555
AjnI	CCWGG	2 cut(s)	1005, 1032
AloI	GAACNNNNNNTCC	2 cut(s)	668, 700
AluBI	AGCT	7 cut(s)	65, 72, 715, 862, 982, 1480, 1486
AluI	AGCT	7 cut(s)	65, 72, 715, 862, 982, 1480, 1486
Alw21I	GWGCWC	6 cut(s)	67, 528, 968, 984, 1014, 1419
Alw26I	GTCTC	3 cut(s)	326, 786, 1531
Alw44I	GTGCAC	1 cut(s)	1415
AlwI	GGATC	1 cut(s)	1001
Ama87I	CYCGRG	2 cut(s)	329, 983
Aor13HI	TCCGGA	1 cut(s)	800
Aor51HI	AGCGCT	1 cut(s)	748
AoxI	GGCC	7 cut(s)	151, 240, 325, 348, 819, 1637, 1682
ApaI	GGGCCC	1 cut(s)	1686
ApaLI	GTGCAC	1 cut(s)	1415
ApeKI	GCWGC	6 cut(s)	408, 715, 720, 809, 927, 1374
ApoI	RAATTY	1 cut(s)	623
Asp700I	GAANNNNTTC	1 cut(s)	1816
AspLEI	GCGC	8 cut(s)	147, 413, 515, 720, 749, 1283, 1285, 1287
AspS9I	GGNCC	9 cut(s)	289, 326, 562, 1003, 1036, 1621, 1637, 1682, 1683
AsuC2I	CCSGG	2 cut(s)	321, 1102
AsuHPI	GGTGA	5 cut(s)	215, 451, 1039, 1223, 1328
AvaI	CYCGRG	2 cut(s)	329, 983
AvaII	GGWCC	5 cut(s)	289, 562, 1003, 1036, 1621
BaeGI	GKGCMC	2 cut(s)	1419, 1686
BalI	TGGCCA	2 cut(s)	153, 242
BanI	GGYRCC	1 cut(s)	512
BanII	GRGCYC	4 cut(s)	67, 984, 1236, 1686
BarI	GAAGNNNNNNTAC	2 cut(s)	1385, 1417
BauI	CACGAG	2 cut(s)	236, 499
BbrPI	CACGTG	2 cut(s)	969, 1015
BbsI	GAAGAC	4 cut(s)	546, 1126, 1196, 1637
Bbv12I	GWGCWC	6 cut(s)	67, 528, 968, 984, 1014, 1419
BbvCI	CCTCAGC	1 cut(s)	1487
BbvI	GCAGC	6 cut(s)	395, 702, 707, 821, 914, 1386
BccI	CCATC	1 cut(s)	1829
BceAI	ACGGC	4 cut(s)	379, 822, 1072, 1270
BcgI	CGANNNNNNTGC	2 cut(s)	697, 731
BciT130I	CCWGG	2 cut(s)	1007, 1034
BclI	TGATCA	1 cut(s)	1176
BcnI	CCSGG	2 cut(s)	321, 1102
BcoDI	GTCTC	3 cut(s)	326, 786, 1531
BfaI	CTAG	2 cut(s)	1076, 1803
BfmI	CTRYAG	2 cut(s)	1625, 1641
BfoI	RGCGCY	2 cut(s)	516, 750
BfuAI	ACCTGC	3 cut(s)	542, 1632, 1634
BglI	GCCNNNNNGGC	1 cut(s)	510
BglII	AGATCT	3 cut(s)	781, 882, 938
BmcAI	AGTACT	1 cut(s)	443
Bme1390I	CCNGG	4 cut(s)	321, 1007, 1034, 1102
Bme18I	GGWCC	5 cut(s)	289, 562, 1003, 1036, 1621
BmeT110I	CYCGRG	2 cut(s)	329, 983
BmgBI	CACGTC	2 cut(s)	1513, 1555
BmgT120I	GGNCC	9 cut(s)	289, 326, 562, 1003, 1036, 1621, 1637, 1682, 1683
BmiI	GGNNCC	5 cut(s)	328, 514, 776, 848, 1684
BmrFI	CCNGG	4 cut(s)	321, 1007, 1034, 1102
BmrI	ACTGGG	1 cut(s)	1644
BmsI	GCATC	2 cut(s)	572, 1266
BmuI	ACTGGG	1 cut(s)	1644
BpiI	GAAGAC	4 cut(s)	546, 1126, 1196, 1637
BplI	GAGNNNNNCTC	2 cut(s)	1148, 1180
BpmI	CTGGAG	1 cut(s)	1028
Bpu10I	CCTNAGC	1 cut(s)	1487
BpuEI	CTTGAG	2 cut(s)	153, 512
BpuMI	CCSGG	2 cut(s)	321, 1102
Bsa29I	ATCGAT	1 cut(s)	872
BsaAI	YACGTR	2 cut(s)	969, 1015
BsaBI	GATNNNNATC	1 cut(s)	1305
BsaHI	GRCGYC	4 cut(s)	366, 513, 959, 1658
BsaI	GGTCTC	1 cut(s)	786
BsaJI	CCNNGG	4 cut(s)	96, 138, 552, 603
BsaWI	WCCGGW	1 cut(s)	800
BsaXI	ACNNNNNCTCC	6 cut(s)	49, 79, 113, 143, 1355, 1385
Bsc4I	CCNNNNNNNGG	7 cut(s)	113, 139, 412, 553, 771, 843, 856
Bse118I	RCCGGY	1 cut(s)	1142
Bse1I	ACTGG	2 cut(s)	154, 1639
Bse8I	GATNNNNATC	1 cut(s)	1305
BseAI	TCCGGA	1 cut(s)	800
BseBI	CCWGG	2 cut(s)	1007, 1034
BseCI	ATCGAT	1 cut(s)	872
BseDI	CCNNGG	4 cut(s)	96, 138, 552, 603
BseGI	GGATG	3 cut(s)	1269, 1783, 1789
BseJI	GATNNNNATC	1 cut(s)	1305
BseLI	CCNNNNNNNGG	7 cut(s)	113, 139, 412, 553, 771, 843, 856
BseMII	CTCAG	8 cut(s)	36, 124, 500, 648, 885, 949, 1249, 1478
BseNI	ACTGG	2 cut(s)	154, 1639
BsePI	GCGCGC	2 cut(s)	1281, 1283
BseRI	GAGGAG	7 cut(s)	64, 74, 96, 923, 1102, 1504, 1592
BseSI	GKGCMC	2 cut(s)	1419, 1686
BseXI	GCAGC	6 cut(s)	395, 702, 707, 821, 914, 1386
BsgI	GTGCAG	1 cut(s)	1393
Bsh1236I	CGCG	6 cut(s)	413, 1130, 1187, 1202, 1283, 1285
Bsh1285I	CGRYCG	1 cut(s)	638
BshFI	GGCC	7 cut(s)	153, 242, 327, 350, 821, 1639, 1684
BshNI	GGYRCC	1 cut(s)	512
BshVI	ATCGAT	1 cut(s)	872
BsiEI	CGRYCG	1 cut(s)	638
BsiHKAI	GWGCWC	6 cut(s)	67, 528, 968, 984, 1014, 1419
BsiHKCI	CYCGRG	2 cut(s)	329, 983
BsiSI	CCGG	5 cut(s)	321, 510, 801, 1101, 1143
BsiWI	CGTACG	2 cut(s)	759, 1401
BslI	CCNNNNNNNGG	7 cut(s)	113, 139, 412, 553, 771, 843, 856
BsmAI	GTCTC	3 cut(s)	326, 786, 1531
BsmBI	CGTCTC	1 cut(s)	326
BsnI	GGCC	7 cut(s)	153, 242, 327, 350, 821, 1639, 1684
Bso31I	GGTCTC	1 cut(s)	786
BsoBI	CYCGRG	2 cut(s)	329, 983
Bsp120I	GGGCCC	1 cut(s)	1682
Bsp1286I	GDGCHC	8 cut(s)	67, 528, 968, 984, 1014, 1236, 1419, 1686
Bsp13I	TCCGGA	1 cut(s)	800
Bsp1407I	TGTACA	2 cut(s)	574, 1611
Bsp19I	CCATGG	3 cut(s)	96, 138, 552
Bsp68I	TCGCGA	1 cut(s)	1187
BspANI	GGCC	7 cut(s)	153, 242, 327, 350, 821, 1639, 1684
BspCNI	CTCAG	8 cut(s)	35, 123, 499, 649, 886, 948, 1248, 1479
BspDI	ATCGAT	1 cut(s)	872
BspEI	TCCGGA	1 cut(s)	800
BspFNI	CGCG	6 cut(s)	413, 1130, 1187, 1202, 1283, 1285
BspLI	GGNNCC	5 cut(s)	328, 514, 776, 848, 1684
BspMAI	CTGCAG	2 cut(s)	1629, 1645
BspMI	ACCTGC	3 cut(s)	542, 1632, 1634
BspPI	GGATC	1 cut(s)	1001
BspQI	GCTCTTC	2 cut(s)	1073, 1115
BspT107I	GGYRCC	1 cut(s)	512
BspTNI	GGTCTC	1 cut(s)	786
BsrFI	RCCGGY	1 cut(s)	1142
BsrGI	TGTACA	2 cut(s)	574, 1611
BsrI	ACTGG	2 cut(s)	154, 1639
BssAI	RCCGGY	1 cut(s)	1142
BssECI	CCNNGG	4 cut(s)	96, 138, 552, 603
BssHII	GCGCGC	2 cut(s)	1281, 1283
BssNI	GRCGYC	4 cut(s)	366, 513, 959, 1658
BssSI	CACGAG	2 cut(s)	236, 499
BssT1I	CCWWGG	4 cut(s)	96, 138, 552, 603
Bst2BI	CACGAG	2 cut(s)	236, 499
Bst2UI	CCWGG	2 cut(s)	1007, 1034
Bst4CI	ACNGT	4 cut(s)	385, 739, 1021, 1195
Bst6I	CTCTTC	4 cut(s)	50, 688, 1073, 1115
BstACI	GRCGYC	4 cut(s)	366, 513, 959, 1658
BstAPI	GCANNNNNTGC	1 cut(s)	1649
BstAUI	TGTACA	2 cut(s)	574, 1611
BstBAI	YACGTR	2 cut(s)	969, 1015
BstC8I	GCNNGC	8 cut(s)	745, 1144, 1264, 1283, 1285, 1419, 1641, 1682
BstDEI	CTNAG	9 cut(s)	22, 110, 486, 657, 894, 935, 1235, 1245, 1487
BstDSI	CCRYGG	3 cut(s)	96, 138, 552
BstF5I	GGATG	3 cut(s)	1269, 1783, 1789
BstFNI	CGCG	6 cut(s)	413, 1130, 1187, 1202, 1283, 1285
BstH2I	RGCGCY	2 cut(s)	516, 750
BstHHI	GCGC	8 cut(s)	147, 413, 515, 720, 749, 1283, 1285, 1287
BstMAI	GTCTC	3 cut(s)	326, 786, 1531
BstMCI	CGRYCG	1 cut(s)	638
BstMWI	GCNNNNNNNGC	6 cut(s)	510, 818, 988, 1263, 1423, 1649
BstNI	CCWGG	2 cut(s)	1007, 1034
BstNSI	RCATGY	1 cut(s)	1463
BstSCI	CCNGG	4 cut(s)	319, 1005, 1032, 1100
BstSFI	CTRYAG	2 cut(s)	1625, 1641
BstSLI	GKGCMC	2 cut(s)	1419, 1686
BstUI	CGCG	6 cut(s)	413, 1130, 1187, 1202, 1283, 1285
BstV1I	GCAGC	6 cut(s)	395, 702, 707, 821, 914, 1386
BstV2I	GAAGAC	4 cut(s)	546, 1126, 1196, 1637
BstX2I	RGATCY	4 cut(s)	781, 882, 938, 993
BstYI	RGATCY	4 cut(s)	781, 882, 938, 993
Bsu15I	ATCGAT	1 cut(s)	872
BsuRI	GGCC	7 cut(s)	153, 242, 327, 350, 821, 1639, 1684
BsuTUI	ATCGAT	1 cut(s)	872
BtgI	CCRYGG	3 cut(s)	96, 138, 552
BtgZI	GCGATG	2 cut(s)	1187, 1243
BtrI	CACGTC	2 cut(s)	1513, 1555
BtsCI	GGATG	3 cut(s)	1269, 1783, 1789
BtsIMutI	CAGTG	5 cut(s)	118, 147, 381, 468, 1388
BtuMI	TCGCGA	1 cut(s)	1187
BveI	ACCTGC	3 cut(s)	542, 1632, 1634
Cac8I	GCNNGC	8 cut(s)	745, 1144, 1264, 1283, 1285, 1419, 1641, 1682
CfoI	GCGC	8 cut(s)	147, 413, 515, 720, 749, 1283, 1285, 1287
Cfr10I	RCCGGY	1 cut(s)	1142
Cfr13I	GGNCC	9 cut(s)	289, 326, 562, 1003, 1036, 1621, 1637, 1682, 1683
ClaI	ATCGAT	1 cut(s)	872
CseI	GACGC	7 cut(s)	355, 574, 948, 1114, 1208, 1394, 1529
Csp6I	GTAC	6 cut(s)	442, 575, 760, 1402, 1454, 1612
CspCI	CAANNNNNGTGG	2 cut(s)	1210, 1245
CviAII	CATG	6 cut(s)	5, 97, 139, 232, 553, 1460
CviQI	GTAC	6 cut(s)	442, 575, 760, 1402, 1454, 1612
DdeI	CTNAG	9 cut(s)	22, 110, 486, 657, 894, 935, 1235, 1245, 1487
DinI	GGCGCC	1 cut(s)	514
DraIII	CACNNNGTG	1 cut(s)	1555
DrdI	GACNNNNNNGTC	2 cut(s)	1034, 1660
DseDI	GACNNNNNNGTC	2 cut(s)	1034, 1660
EaeI	YGGCCR	4 cut(s)	151, 240, 348, 819
Eam1104I	CTCTTC	4 cut(s)	50, 688, 1073, 1115
EarI	CTCTTC	4 cut(s)	50, 688, 1073, 1115
EciI	GGCGGA	3 cut(s)	431, 572, 1006
Ecl136II	GAGCTC	2 cut(s)	65, 982
Eco130I	CCWWGG	4 cut(s)	96, 138, 552, 603
Eco24I	GRGCYC	4 cut(s)	67, 984, 1236, 1686
Eco31I	GGTCTC	1 cut(s)	786
Eco32I	GATATC	1 cut(s)	175
Eco47I	GGWCC	5 cut(s)	289, 562, 1003, 1036, 1621
Eco47III	AGCGCT	1 cut(s)	748
Eco53kI	GAGCTC	2 cut(s)	65, 982
Eco57I	CTGAAG	2 cut(s)	492, 1412
Eco72I	CACGTG	2 cut(s)	969, 1015
Eco88I	CYCGRG	2 cut(s)	329, 983
EcoICRI	GAGCTC	2 cut(s)	65, 982
EcoO109I	RGGNCCY	2 cut(s)	326, 562
EcoRII	CCWGG	2 cut(s)	1005, 1032
EcoRV	GATATC	1 cut(s)	175
EcoT14I	CCWWGG	4 cut(s)	96, 138, 552, 603
EcoT22I	ATGCAT	2 cut(s)	6, 1465
EcoT38I	GRGCYC	4 cut(s)	67, 984, 1236, 1686
EgeI	GGCGCC	1 cut(s)	514
EheI	GGCGCC	1 cut(s)	514
ErhI	CCWWGG	4 cut(s)	96, 138, 552, 603
Esp3I	CGTCTC	1 cut(s)	326
FaeI	CATG	6 cut(s)	8, 100, 142, 235, 556, 1463
FalI	AAGNNNNNCTT	2 cut(s)	531, 563
FatI	CATG	6 cut(s)	4, 96, 138, 231, 552, 1459
FauI	CCCGC	1 cut(s)	1673
FbaI	TGATCA	1 cut(s)	1176
FblI	GTMKAC	3 cut(s)	1023, 1197, 1293
FokI	GGATG	3 cut(s)	1256, 1790, 1796
FriOI	GRGCYC	4 cut(s)	67, 984, 1236, 1686
FspBI	CTAG	2 cut(s)	1076, 1803
GlaI	GCGC	8 cut(s)	146, 412, 514, 719, 748, 1282, 1284, 1286
GsuI	CTGGAG	1 cut(s)	1028
HaeII	RGCGCY	2 cut(s)	516, 750
HaeIII	GGCC	7 cut(s)	153, 242, 327, 350, 821, 1639, 1684
HapII	CCGG	5 cut(s)	321, 510, 801, 1101, 1143
HgaI	GACGC	7 cut(s)	355, 574, 948, 1114, 1208, 1394, 1529
HhaI	GCGC	8 cut(s)	147, 413, 515, 720, 749, 1283, 1285, 1287
Hin1I	GRCGYC	4 cut(s)	366, 513, 959, 1658
Hin1II	CATG	6 cut(s)	8, 100, 142, 235, 556, 1463
Hin6I	GCGC	8 cut(s)	145, 411, 513, 718, 747, 1281, 1283, 1285
HinP1I	GCGC	8 cut(s)	145, 411, 513, 718, 747, 1281, 1283, 1285
HincII	GTYRAC	4 cut(s)	370, 1024, 1198, 1294
HindII	GTYRAC	4 cut(s)	370, 1024, 1198, 1294
HinfI	GANTC	7 cut(s)	8, 275, 300, 631, 833, 869, 1182
HpaII	CCGG	5 cut(s)	321, 510, 801, 1101, 1143
HphI	GGTGA	5 cut(s)	215, 451, 1039, 1223, 1328
Hpy166II	GTNNAC	8 cut(s)	370, 388, 654, 1024, 1047, 1198, 1294, 1417
Hpy8I	GTNNAC	8 cut(s)	370, 388, 654, 1024, 1047, 1198, 1294, 1417
HpyAV	CCTTC	9 cut(s)	337, 557, 1108, 1324, 1354, 1360, 1750, 1750, 1806
HpyCH4III	ACNGT	4 cut(s)	385, 739, 1021, 1195
HpyCH4IV	ACGT	8 cut(s)	335, 393, 758, 968, 1014, 1512, 1554, 1658
HpyF10VI	GCNNNNNNNGC	6 cut(s)	510, 818, 988, 1263, 1423, 1649
HpyF3I	CTNAG	9 cut(s)	22, 110, 486, 657, 894, 935, 1235, 1245, 1487
HpySE526I	ACGT	8 cut(s)	335, 393, 758, 968, 1014, 1512, 1554, 1658
Hsp92I	GRCGYC	4 cut(s)	366, 513, 959, 1658
Hsp92II	CATG	6 cut(s)	8, 100, 142, 235, 556, 1463
HspAI	GCGC	8 cut(s)	145, 411, 513, 718, 747, 1281, 1283, 1285
KasI	GGCGCC	1 cut(s)	512
Kpn2I	TCCGGA	1 cut(s)	800
KroI	GCCGGC	1 cut(s)	1142
KroNI	GCCGGC	1 cut(s)	1144
Ksp22I	TGATCA	1 cut(s)	1176
LguI	GCTCTTC	2 cut(s)	1073, 1115
LmnI	GCTCC	7 cut(s)	70, 77, 142, 780, 852, 859, 1009
Lsp1109I	GCAGC	6 cut(s)	395, 702, 707, 821, 914, 1386
LweI	GCATC	2 cut(s)	572, 1266
MaeI	CTAG	2 cut(s)	1076, 1803
MaeII	ACGT	8 cut(s)	335, 393, 758, 968, 1014, 1512, 1554, 1658
MaeIII	GTNAC	3 cut(s)	26, 359, 1015
MflI	RGATCY	4 cut(s)	781, 882, 938, 993
MhlI	GDGCHC	8 cut(s)	67, 528, 968, 984, 1014, 1236, 1419, 1686
MlsI	TGGCCA	2 cut(s)	153, 242
MluCI	AATT	9 cut(s)	15, 211, 570, 623, 646, 662, 815, 1449, 1721
MluI	ACGCGT	1 cut(s)	1200
MluNI	TGGCCA	2 cut(s)	153, 242
Mly113I	GGCGCC	1 cut(s)	513
MlyI	GAGTC	4 cut(s)	284, 640, 827, 1191
MmeI	TCCRAC	1 cut(s)	874
Mox20I	TGGCCA	2 cut(s)	153, 242
Mph1103I	ATGCAT	2 cut(s)	6, 1465
MreI	CGCCGGCG	1 cut(s)	1142
MroI	TCCGGA	1 cut(s)	800
MroNI	GCCGGC	1 cut(s)	1142
MroXI	GAANNNNTTC	1 cut(s)	1816
MscI	TGGCCA	2 cut(s)	153, 242
MseI	TTAA	4 cut(s)	482, 915, 1733, 1828
MslI	CAYNNNNRTG	2 cut(s)	236, 521
Msp20I	TGGCCA	2 cut(s)	153, 242
MspA1I	CMGCKG	1 cut(s)	408
MspI	CCGG	5 cut(s)	321, 510, 801, 1101, 1143
MspR9I	CCNGG	4 cut(s)	321, 1007, 1034, 1102
MvaI	CCWGG	2 cut(s)	1007, 1034
MvnI	CGCG	6 cut(s)	413, 1130, 1187, 1202, 1283, 1285
MwoI	GCNNNNNNNGC	6 cut(s)	510, 818, 988, 1263, 1423, 1649
NaeI	GCCGGC	1 cut(s)	1144
NarI	GGCGCC	1 cut(s)	513
NciI	CCSGG	2 cut(s)	321, 1102
NcoI	CCATGG	3 cut(s)	96, 138, 552
NgoMIV	GCCGGC	1 cut(s)	1142
NlaIII	CATG	6 cut(s)	8, 100, 142, 235, 556, 1463
NlaIV	GGNNCC	5 cut(s)	328, 514, 776, 848, 1684
NmeAIII	GCCGAG	3 cut(s)	790, 977, 1145
NmuCI	GTSAC	2 cut(s)	26, 1015
NruI	TCGCGA	1 cut(s)	1187
NsiI	ATGCAT	2 cut(s)	6, 1465
NspI	RCATGY	1 cut(s)	1463
PaeR7I	CTCGAG	1 cut(s)	983
PaqCI	CACCTGC	1 cut(s)	1634
PauI	GCGCGC	2 cut(s)	1281, 1283
PciSI	GCTCTTC	2 cut(s)	1073, 1115
PcsI	WCGNNNNNNNCGW	5 cut(s)	293, 1020, 1406, 1661, 1712
PdiI	GCCGGC	1 cut(s)	1144
PdmI	GAANNNNTTC	1 cut(s)	1816
PfeI	GAWTC	3 cut(s)	8, 300, 869
Pfl23II	CGTACG	2 cut(s)	759, 1401
PflFI	GACNNNGTC	2 cut(s)	1657, 1660
PfoI	TCCNGGA	2 cut(s)	319, 1005
Ple19I	CGATCG	1 cut(s)	638
PleI	GAGTC	4 cut(s)	283, 639, 827, 1190
PluTI	GGCGCC	1 cut(s)	516
PmaCI	CACGTG	2 cut(s)	969, 1015
PmlI	CACGTG	2 cut(s)	969, 1015
PpsI	GAGTC	4 cut(s)	283, 639, 827, 1190
Ppu21I	YACGTR	2 cut(s)	969, 1015
PpuMI	RGGWCCY	1 cut(s)	562
Psp124BI	GAGCTC	2 cut(s)	67, 984
Psp5II	RGGWCCY	1 cut(s)	562
Psp6I	CCWGG	2 cut(s)	1005, 1032
PspCI	CACGTG	2 cut(s)	969, 1015
PspGI	CCWGG	2 cut(s)	1005, 1032
PspLI	CGTACG	2 cut(s)	759, 1401
PspN4I	GGNNCC	5 cut(s)	328, 514, 776, 848, 1684
PspOMI	GGGCCC	1 cut(s)	1682
PspPI	GGNCC	9 cut(s)	289, 326, 562, 1003, 1036, 1621, 1637, 1682, 1683
PspPPI	RGGWCCY	1 cut(s)	562
PstI	CTGCAG	2 cut(s)	1629, 1645
PsuI	RGATCY	4 cut(s)	781, 882, 938, 993
PsyI	GACNNNGTC	2 cut(s)	1657, 1660
PteI	GCGCGC	2 cut(s)	1281, 1283
PvuI	CGATCG	1 cut(s)	638
RruI	TCGCGA	1 cut(s)	1187
RsaI	GTAC	6 cut(s)	443, 576, 761, 1403, 1455, 1613
RsaNI	GTAC	6 cut(s)	442, 575, 760, 1402, 1454, 1612
RseI	CAYNNNNRTG	2 cut(s)	236, 521
SacI	GAGCTC	2 cut(s)	67, 984
SalI	GTCGAC	3 cut(s)	1022, 1196, 1292
SapI	GCTCTTC	2 cut(s)	1073, 1115
SaqAI	TTAA	4 cut(s)	482, 915, 1733, 1828
Sau96I	GGNCC	9 cut(s)	289, 326, 562, 1003, 1036, 1621, 1637, 1682, 1683
SbfI	CCTGCAGG	1 cut(s)	1645
ScaI	AGTACT	1 cut(s)	443
SchI	GAGTC	4 cut(s)	284, 640, 827, 1191
ScrFI	CCNGG	4 cut(s)	321, 1007, 1034, 1102
SdaI	CCTGCAGG	1 cut(s)	1645
SduI	GDGCHC	8 cut(s)	67, 528, 968, 984, 1014, 1236, 1419, 1686
SfaNI	GCATC	2 cut(s)	572, 1266
SfcI	CTRYAG	2 cut(s)	1625, 1641
SfoI	GGCGCC	1 cut(s)	514
Sfr274I	CTCGAG	1 cut(s)	983
SgrAI	CRCCGGYG	1 cut(s)	1142
SgrDI	CGTCGACG	1 cut(s)	1022
SinI	GGWCC	5 cut(s)	289, 562, 1003, 1036, 1621
SlaI	CTCGAG	1 cut(s)	983
SmiMI	CAYNNNNRTG	2 cut(s)	236, 521
SmlI	CTYRAG	3 cut(s)	168, 527, 983
SmoI	CTYRAG	3 cut(s)	168, 527, 983
Sse8387I	CCTGCAGG	1 cut(s)	1645
Sse9I	AATT	9 cut(s)	15, 211, 570, 623, 646, 662, 815, 1449, 1721
SspDI	GGCGCC	1 cut(s)	512
SspMI	CTAG	2 cut(s)	1076, 1803
SstI	GAGCTC	2 cut(s)	67, 984
StyD4I	CCNGG	4 cut(s)	319, 1005, 1032, 1100
StyI	CCWWGG	4 cut(s)	96, 138, 552, 603
TaaI	ACNGT	4 cut(s)	385, 739, 1021, 1195
TaiI	ACGT	8 cut(s)	338, 396, 761, 971, 1017, 1515, 1557, 1661
TaqII	GACCGA	2 cut(s)	449, 992
TasI	AATT	9 cut(s)	15, 211, 570, 623, 646, 662, 815, 1449, 1721
TatI	WGTACW	4 cut(s)	441, 574, 1453, 1611
TauI	GCSGC	8 cut(s)	120, 350, 401, 416, 468, 824, 847, 1172
TfiI	GAWTC	3 cut(s)	8, 300, 869
Tru1I	TTAA	4 cut(s)	482, 915, 1733, 1828
Tru9I	TTAA	4 cut(s)	482, 915, 1733, 1828
TscAI	CASTG	5 cut(s)	118, 154, 388, 475, 1395
TseFI	GTSAC	2 cut(s)	26, 1015
TseI	GCWGC	6 cut(s)	408, 715, 720, 809, 927, 1374
Tsp45I	GTSAC	2 cut(s)	26, 1015
TspDTI	ATGAA	5 cut(s)	21, 220, 725, 1619, 1781
TspGWI	ACGGA	2 cut(s)	1604, 1634
TspRI	CASTG	5 cut(s)	118, 154, 388, 475, 1395
Tth111I	GACNNNGTC	2 cut(s)	1657, 1660
VneI	GTGCAC	1 cut(s)	1415
VpaK11BI	GGWCC	5 cut(s)	289, 562, 1003, 1036, 1621
XapI	RAATTY	1 cut(s)	623
XbaI	TCTAGA	1 cut(s)	1075
XceI	RCATGY	1 cut(s)	1463
XhoI	CTCGAG	1 cut(s)	983
XmiI	GTMKAC	3 cut(s)	1023, 1197, 1293
XmnI	GAANNNNTTC	1 cut(s)	1816
XspI	CTAG	2 cut(s)	1076, 1803
ZraI	GACGTC	1 cut(s)	1659
ZrmI	AGTACT	1 cut(s)	443
Zsp2I	ATGCAT	2 cut(s)	6, 1465

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.