Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005865

Physical Location & Seq

Genomic Coordinates Forward (+)

4400251 .. 4403706

Gene Structure 3456 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1051400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGCAACCCGCAATCGTGACCCCATTTATTCAGAGACCATATAATCTCTCTCTTCTCTCTCTGCACAGTAGCTCCAAGCTCCGCCCACCTCACATGGCTTCCCCTCTCAATGGCAGCAACAGGCTCTCCCTTGCCATGGAGCGCACTGGCCAATGGATTTTCTCTCAAGATATCCCGTCAGATGTTGTGGTTCAAGTTGGTGAAGCCAATTTCTCTCTACACAAGTTCATGCTTGTGGCGAAAAGCAACCGCATAAGGAAGCTGATAATGGAGTCTAAAAAACCGGACCTAACGAGAATCGACCTCTCCGACGTTCCCGGAGGCCCGGAGACGTTCGAGAAGGCGGCCAAGTTCTGCTACGGCGTCAACTTCGAGATCACAGTACACAACGTCGCCGCCCTCCGTTGCGCGGCGGAGTATTTGGAAATGACAGAAAAGTACTGCGACAACAACCTCACCGGCCGCACTGAAGATTTCTTATCTCAAGTCGCTCTCATGAGCCTCTCCGGTGCCATTGTCGTGCTCAAGTCCTGCGAAGATCTCCTTCCCATGGCGGAGGACCTCAAAATCGTACAGAAATGCGTCGATGTCGCCGCTTCCAAGGCCTCAATCGAAGCAAAATTTCCGAGTCGATCGCCGACGAATTGGTGGACAGAGGAATTGTCGATTTTGGACATTGAGTTCTTCGGCAGAGTTATCACCGCGATGAAGCTGCGCGGCGGGAAATCATTGACCGTTTCCAGCGCTATCATAACTTACGCTGAGAAATGGCTCCGAGATATCGTCCGAGACCGCTCTGGACCCGCCGCGAAATTGGCCGCTTCGGACGACTCCGATCTGCGACTCCAACAACGGGAGCTTCTGGAATCGATCGTCGCGATCTTACCGACTGAGAAAGCTGCAATGCCGATCAACTTCCTCTGCTGCCTTCTCAGATCTGCGACCTTCGTCAAGGCGTCGAGCGCGTGCAAGACCGAGCTCGAGAGGCGGATCTCGTCGGTCCTGGAGCACGTGACCGTCGACGACCTCCTGGTACTGTCGTTCACCTACGACGGCGAGAGGCTGTTCGATCTCGAGAGCGTGAGGAGGATTATCTCCGGGTTCGTGGAGAAGGAGAAGAGCGTCGCGGTCTTCAACGCCGGCGATTTCAGGGAGGTTTGCTCGGCGGAGATGATCAGAGTCGCGAAGACTGTCGACGCGTATCTTGGTGAGATCGCCACGTGTGATGAGCTCAGTATCTCGAAGTTCAATGGCATTGCCAACCTCATCCCCAAAGGCGCGCGTAAGGTCGACGATGATCTCTATCGTGCTATTGATATTTACTTGAAGGCCCATCCGAACCTTGATGAGATAGAGCGGGAGAAGGTGTGCAGCGTAATGGACGCATTGAAGTTGTCATACGAAGCGAGAGTCCACGCTTCACAAAACAAACGACTCCCCGTTCAAATCGTACTGCACGCGTTGTATTACGACCAGCTAAAGCTGAGGAGCGGGGTGGACGAAAACGACGTGCAAGACGCAATAACGACGAGGAGTCAGGTGCAACACGACGTGTCGTTGGCGAGGGAAAACGAGGAGTTGAGAACGGAGTTGTTGAAGATGAAGATGTACATTACGGACCTGCAGAAGACTGGTTCTGCAGGGATTGCAACGACGTCGTCTGGTAAAGGAGGTGGCGGGCCCAAAAAGGCCACATTTTTCTCTTCTGTGTCGAAGAAATTGGGGAAGTTGAATCCCTTCAAGCAAGGGTCGAAGGACACATCGAATATCATAGATGATGGTGTGGATATTTCCAAGCCTAGAAGGAGAAGGTTTTCTATTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

609

Amino Acids

67.59

Weight (kDa)

8.15

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	55 - 145	2.4e-08	BTB/POZ domain
NPH3	PF03000	215 - 469	1.1e-79	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005865)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30520 AT2G30520 AT2G30520
fragaria_vesca	FvH4_3g40860 FvH4_3g40860 FvH4_3g40860 FvH4_3g40860
malus_domestica	MD03G1048900.v1.1 MD11G1051400.v1.1
prunus_persica	Prupe.6G039200_v2.0.a1 Prupe.6G039200_v2.0.a1
pyrus_communis	pycom03g03840 pycom11g04120
rosa_chinensis	RchiOBHm_Chr5g0073591
rosa_laevigata	RLG00000036398
rosa_multiflora	Rmu_co8263269.1_g000001 Rmu_co8430755.1_g000001 Rmu_sc0042142.1_g000002 Rmu_ssc0000242.1_g000005
rosa_roxburghii	Rroxscaffold_1G00007670
rosa_rugosa	Rorug05G0425200 Rorug05G0425300 Rorug05G0425400
rosa_samantha	Rh5AG482200 Rh5BG503000 Rh5CG526100 Rh5DG515200
rosa_wichuraiana	Rw5G044800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1658
Acc36I	ACCTGC	1 cut(s)	1629
AccB1I	GGYRCC	1 cut(s)	509
AccBSI	CCGCTC	3 cut(s)	795, 1357, 1491
AccI	GTMKAC	3 cut(s)	1020, 1194, 1290
AclWI	GGATC	1 cut(s)	998
AcoI	YGGCCR	4 cut(s)	148, 345, 460, 816
AcsI	RAATTY	1 cut(s)	620
AcuI	CTGAAG	1 cut(s)	489
AcvI	CACGTG	2 cut(s)	1012, 1221
AcyI	GRCGYC	3 cut(s)	363, 956, 1655
AdeI	CACNNNGTG	1 cut(s)	1552
AfaI	GTAC	6 cut(s)	384, 440, 573, 1035, 1452, 1610
AfeI	AGCGCT	1 cut(s)	745
AfiI	CCNNNNNNNGG	5 cut(s)	110, 136, 409, 550, 853
AflIII	ACRYGT	4 cut(s)	1197, 1220, 1458, 1551
AgsI	TTSAA	9 cut(s)	194, 1135, 1249, 1327, 1390, 1445, 1597, 1732, 1741
AhdI	GACNNNNNGTC	1 cut(s)	1533
AjiI	CACGTC	2 cut(s)	1510, 1552
AjnI	CCWGG	2 cut(s)	1002, 1029
AloI	GAACNNNNNNTCC	2 cut(s)	665, 697
Alw21I	GWGCWC	4 cut(s)	525, 981, 1011, 1233
Alw26I	GTCTC	3 cut(s)	28, 323, 783
AlwI	GGATC	1 cut(s)	998
Ama87I	CYCGRG	2 cut(s)	980, 1073
Aor51HI	AGCGCT	1 cut(s)	745
AoxI	GGCC	9 cut(s)	148, 322, 345, 460, 603, 816, 1329, 1679, 1689
ApaI	GGGCCC	1 cut(s)	1683
ApeKI	GCWGC	5 cut(s)	114, 712, 899, 924, 1371
ApoI	RAATTY	1 cut(s)	620
ArsI	GACNNNNNNTTYG	2 cut(s)	1395, 1427
Asp700I	GAANNNNTTC	2 cut(s)	1736, 1813
AspLEI	GCGC	7 cut(s)	144, 410, 717, 746, 965, 1280, 1282
AspS9I	GGNCC	9 cut(s)	286, 323, 559, 800, 1000, 1330, 1618, 1679, 1680
AsuC2I	CCSGG	3 cut(s)	318, 326, 1099
AsuHPI	GGTGA	5 cut(s)	212, 448, 691, 1036, 1220
AvaI	CYCGRG	2 cut(s)	980, 1073
AvaII	GGWCC	5 cut(s)	286, 559, 800, 1000, 1618
BaeGI	GKGCMC	1 cut(s)	1683
BaeI	ACNNNNGTAYC	2 cut(s)	1025, 1058
BalI	TGGCCA	1 cut(s)	150
BanI	GGYRCC	1 cut(s)	509
BanII	GRGCYC	3 cut(s)	981, 1233, 1683
BarI	GAAGNNNNNNTAC	2 cut(s)	1382, 1414
BbrPI	CACGTG	2 cut(s)	1012, 1221
BbsI	GAAGAC	3 cut(s)	1123, 1193, 1634
Bbv12I	GWGCWC	4 cut(s)	525, 981, 1011, 1233
BbvCI	CCTCAGC	1 cut(s)	1484
BbvI	GCAGC	5 cut(s)	126, 699, 886, 911, 1383
BccI	CCATC	2 cut(s)	1341, 1772
BceAI	ACGGC	2 cut(s)	376, 1069
BcgI	CGANNNNNNTGC	4 cut(s)	694, 728, 1638, 1672
BciT130I	CCWGG	2 cut(s)	1004, 1031
BclI	TGATCA	1 cut(s)	1173
BcnI	CCSGG	3 cut(s)	318, 326, 1099
BcoDI	GTCTC	3 cut(s)	28, 323, 783
BfaI	CTAG	1 cut(s)	1800
BfmI	CTRYAG	2 cut(s)	1622, 1638
BfoI	RGCGCY	1 cut(s)	747
BfuAI	ACCTGC	1 cut(s)	1629
BglII	AGATCT	2 cut(s)	538, 935
BmcAI	AGTACT	1 cut(s)	440
Bme1390I	CCNGG	5 cut(s)	318, 326, 1004, 1031, 1099
Bme18I	GGWCC	5 cut(s)	286, 559, 800, 1000, 1618
BmeRI	GACNNNNNGTC	1 cut(s)	1533
BmeT110I	CYCGRG	2 cut(s)	980, 1073
BmgBI	CACGTC	2 cut(s)	1510, 1552
BmgT120I	GGNCC	9 cut(s)	286, 323, 559, 800, 1000, 1330, 1618, 1679, 1680
BmiI	GGNNCC	4 cut(s)	511, 773, 802, 1681
BmrFI	CCNGG	5 cut(s)	318, 326, 1004, 1031, 1099
BoxI	GACNNNNGTC	1 cut(s)	947
BpiI	GAAGAC	3 cut(s)	1123, 1193, 1634
BplI	GAGNNNNNCTC	2 cut(s)	1145, 1177
BpmI	CTGGAG	1 cut(s)	1025
Bpu10I	CCTNAGC	1 cut(s)	1484
BpuEI	CTTGAG	3 cut(s)	150, 468, 509
BpuMI	CCSGG	3 cut(s)	318, 326, 1099
Bsa29I	ATCGAT	1 cut(s)	869
BsaAI	YACGTR	2 cut(s)	1012, 1221
BsaBI	GATNNNNATC	1 cut(s)	1302
BsaHI	GRCGYC	3 cut(s)	363, 956, 1655
BsaI	GGTCTC	2 cut(s)	28, 783
BsaJI	CCNNGG	3 cut(s)	135, 549, 600
BsaWI	WCCGGW	2 cut(s)	283, 506
BsaXI	ACNNNNNCTCC	6 cut(s)	56, 86, 110, 140, 1352, 1382
Bsc4I	CCNNNNNNNGG	5 cut(s)	110, 136, 409, 550, 853
Bse118I	RCCGGY	2 cut(s)	458, 1139
Bse1I	ACTGG	2 cut(s)	151, 1636
Bse3DI	GCAATG	2 cut(s)	909, 1254
Bse8I	GATNNNNATC	1 cut(s)	1302
BseBI	CCWGG	2 cut(s)	1004, 1031
BseCI	ATCGAT	1 cut(s)	869
BseDI	CCNNGG	3 cut(s)	135, 549, 600
BseGI	GGATG	2 cut(s)	1266, 1333
BseJI	GATNNNNATC	1 cut(s)	1302
BseLI	CCNNNNNNNGG	5 cut(s)	110, 136, 409, 550, 853
BseMI	GCAATG	2 cut(s)	909, 1254
BseMII	CTCAG	5 cut(s)	753, 882, 946, 1246, 1475
BseNI	ACTGG	2 cut(s)	151, 1636
BsePI	GCGCGC	1 cut(s)	1278
BseRI	GAGGAG	4 cut(s)	1099, 1501, 1546, 1589
BseSI	GKGCMC	1 cut(s)	1683
BseX3I	CGGCCG	1 cut(s)	460
BseXI	GCAGC	5 cut(s)	126, 699, 886, 911, 1383
BsgI	GTGCAG	3 cut(s)	47, 1390, 1439
Bsh1285I	CGRYCG	3 cut(s)	463, 635, 873
BshFI	GGCC	9 cut(s)	150, 324, 347, 462, 605, 818, 1331, 1681, 1691
BshNI	GGYRCC	1 cut(s)	509
BshVI	ATCGAT	1 cut(s)	869
BsiEI	CGRYCG	3 cut(s)	463, 635, 873
BsiHKAI	GWGCWC	4 cut(s)	525, 981, 1011, 1233
BsiHKCI	CYCGRG	2 cut(s)	980, 1073
BsiSI	CCGG	7 cut(s)	284, 318, 326, 459, 507, 1098, 1140
BslI	CCNNNNNNNGG	5 cut(s)	110, 136, 409, 550, 853
BsmAI	GTCTC	3 cut(s)	28, 323, 783
BsmBI	CGTCTC	1 cut(s)	323
BsnI	GGCC	9 cut(s)	150, 324, 347, 462, 605, 818, 1331, 1681, 1691
Bso31I	GGTCTC	2 cut(s)	28, 783
BsoBI	CYCGRG	2 cut(s)	980, 1073
Bsp120I	GGGCCC	1 cut(s)	1679
Bsp1286I	GDGCHC	5 cut(s)	525, 981, 1011, 1233, 1683
Bsp1407I	TGTACA	1 cut(s)	1608
Bsp19I	CCATGG	2 cut(s)	135, 549
Bsp68I	TCGCGA	2 cut(s)	878, 1184
BspANI	GGCC	9 cut(s)	150, 324, 347, 462, 605, 818, 1331, 1681, 1691
BspCNI	CTCAG	5 cut(s)	754, 883, 945, 1245, 1476
BspDI	ATCGAT	1 cut(s)	869
BspHI	TCATGA	1 cut(s)	495
BspLI	GGNNCC	4 cut(s)	511, 773, 802, 1681
BspMAI	CTGCAG	2 cut(s)	1626, 1642
BspMI	ACCTGC	1 cut(s)	1629
BspPI	GGATC	1 cut(s)	998
BspQI	GCTCTTC	1 cut(s)	1112
BspT107I	GGYRCC	1 cut(s)	509
BspTNI	GGTCTC	2 cut(s)	28, 783
BsrBI	CCGCTC	3 cut(s)	795, 1357, 1491
BsrDI	GCAATG	2 cut(s)	909, 1254
BsrFI	RCCGGY	2 cut(s)	458, 1139
BsrGI	TGTACA	1 cut(s)	1608
BsrI	ACTGG	2 cut(s)	151, 1636
BssAI	RCCGGY	2 cut(s)	458, 1139
BssECI	CCNNGG	3 cut(s)	135, 549, 600
BssHII	GCGCGC	1 cut(s)	1278
BssNI	GRCGYC	3 cut(s)	363, 956, 1655
BssT1I	CCWWGG	3 cut(s)	135, 549, 600
Bst2UI	CCWGG	2 cut(s)	1004, 1031
Bst4CI	ACNGT	6 cut(s)	68, 382, 736, 1018, 1038, 1192
Bst6I	CTCTTC	3 cut(s)	57, 1112, 1708
BstACI	GRCGYC	3 cut(s)	363, 956, 1655
BstAPI	GCANNNNNTGC	1 cut(s)	1646
BstAUI	TGTACA	1 cut(s)	1608
BstBAI	YACGTR	2 cut(s)	1012, 1221
BstC8I	GCNNGC	5 cut(s)	967, 1141, 1280, 1458, 1679
BstDEI	CTNAG	5 cut(s)	762, 891, 932, 1232, 1484
BstDSI	CCRYGG	2 cut(s)	135, 549
BstF5I	GGATG	2 cut(s)	1266, 1333
BstH2I	RGCGCY	1 cut(s)	747
BstHHI	GCGC	7 cut(s)	144, 410, 717, 746, 965, 1280, 1282
BstMAI	GTCTC	3 cut(s)	28, 323, 783
BstMCI	CGRYCG	3 cut(s)	463, 635, 873
BstMWI	GCNNNNNNNGC	5 cut(s)	602, 815, 962, 985, 1646
BstNI	CCWGG	2 cut(s)	1004, 1031
BstPAI	GACNNNNGTC	1 cut(s)	947
BstSCI	CCNGG	5 cut(s)	316, 324, 1002, 1029, 1097
BstSFI	CTRYAG	2 cut(s)	1622, 1638
BstSLI	GKGCMC	1 cut(s)	1683
BstV1I	GCAGC	5 cut(s)	126, 699, 886, 911, 1383
BstV2I	GAAGAC	3 cut(s)	1123, 1193, 1634
BstX2I	RGATCY	3 cut(s)	538, 935, 990
BstYI	RGATCY	3 cut(s)	538, 935, 990
BstZI	CGGCCG	1 cut(s)	460
Bsu15I	ATCGAT	1 cut(s)	869
BsuRI	GGCC	9 cut(s)	150, 324, 347, 462, 605, 818, 1331, 1681, 1691
BsuTUI	ATCGAT	1 cut(s)	869
BtgI	CCRYGG	2 cut(s)	135, 549
BtgZI	GCGATG	1 cut(s)	719
BtrI	CACGTC	2 cut(s)	1510, 1552
BtsCI	GGATG	2 cut(s)	1266, 1333
BtsIMutI	CAGTG	2 cut(s)	144, 465
BtuMI	TCGCGA	2 cut(s)	878, 1184
BveI	ACCTGC	1 cut(s)	1629
Cac8I	GCNNGC	5 cut(s)	967, 1141, 1280, 1458, 1679
CciI	TCATGA	1 cut(s)	495
CfoI	GCGC	7 cut(s)	144, 410, 717, 746, 965, 1280, 1282
Cfr10I	RCCGGY	2 cut(s)	458, 1139
Cfr13I	GGNCC	9 cut(s)	286, 323, 559, 800, 1000, 1330, 1618, 1679, 1680
ClaI	ATCGAT	1 cut(s)	869
CseI	GACGC	7 cut(s)	352, 571, 945, 1111, 1205, 1391, 1526
Csp6I	GTAC	6 cut(s)	383, 439, 572, 1034, 1451, 1609
CviAII	CATG	5 cut(s)	94, 136, 229, 496, 550
CviQI	GTAC	6 cut(s)	383, 439, 572, 1034, 1451, 1609
DdeI	CTNAG	5 cut(s)	762, 891, 932, 1232, 1484
DraIII	CACNNNGTG	1 cut(s)	1552
DriI	GACNNNNNGTC	1 cut(s)	1533
EaeI	YGGCCR	4 cut(s)	148, 345, 460, 816
EagI	CGGCCG	1 cut(s)	460
Eam1104I	CTCTTC	3 cut(s)	57, 1112, 1708
Eam1105I	GACNNNNNGTC	1 cut(s)	1533
EarI	CTCTTC	3 cut(s)	57, 1112, 1708
EciI	GGCGGA	5 cut(s)	71, 428, 569, 1003, 1181
Ecl136II	GAGCTC	2 cut(s)	979, 1231
EclXI	CGGCCG	1 cut(s)	460
Eco130I	CCWWGG	3 cut(s)	135, 549, 600
Eco147I	AGGCCT	1 cut(s)	605
Eco24I	GRGCYC	3 cut(s)	981, 1233, 1683
Eco31I	GGTCTC	2 cut(s)	28, 783
Eco32I	GATATC	2 cut(s)	172, 781
Eco47I	GGWCC	5 cut(s)	286, 559, 800, 1000, 1618
Eco47III	AGCGCT	1 cut(s)	745
Eco52I	CGGCCG	1 cut(s)	460
Eco53kI	GAGCTC	2 cut(s)	979, 1231
Eco57I	CTGAAG	1 cut(s)	489
Eco72I	CACGTG	2 cut(s)	1012, 1221
Eco88I	CYCGRG	2 cut(s)	980, 1073
EcoICRI	GAGCTC	2 cut(s)	979, 1231
EcoO109I	RGGNCCY	1 cut(s)	559
EcoRII	CCWGG	2 cut(s)	1002, 1029
EcoRV	GATATC	2 cut(s)	172, 781
EcoT14I	CCWWGG	3 cut(s)	135, 549, 600
EcoT38I	GRGCYC	3 cut(s)	981, 1233, 1683
ErhI	CCWWGG	3 cut(s)	135, 549, 600
Esp3I	CGTCTC	1 cut(s)	323
FaeI	CATG	5 cut(s)	97, 139, 232, 499, 553
FalI	AAGNNNNNCTT	2 cut(s)	528, 560
FatI	CATG	5 cut(s)	93, 135, 228, 495, 549
FauI	CCCGC	6 cut(s)	16, 713, 811, 1350, 1484, 1670
FbaI	TGATCA	1 cut(s)	1173
FblI	GTMKAC	3 cut(s)	1020, 1194, 1290
FokI	GGATG	2 cut(s)	1253, 1320
FriOI	GRGCYC	3 cut(s)	981, 1233, 1683
FspBI	CTAG	1 cut(s)	1800
GlaI	GCGC	7 cut(s)	143, 409, 716, 745, 964, 1279, 1281
GsuI	CTGGAG	1 cut(s)	1025
HaeII	RGCGCY	1 cut(s)	747
HaeIII	GGCC	9 cut(s)	150, 324, 347, 462, 605, 818, 1331, 1681, 1691
HapII	CCGG	7 cut(s)	284, 318, 326, 459, 507, 1098, 1140
HgaI	GACGC	7 cut(s)	352, 571, 945, 1111, 1205, 1391, 1526
HhaI	GCGC	7 cut(s)	144, 410, 717, 746, 965, 1280, 1282
Hin1I	GRCGYC	3 cut(s)	363, 956, 1655
Hin1II	CATG	5 cut(s)	97, 139, 232, 499, 553
Hin6I	GCGC	7 cut(s)	142, 408, 715, 744, 963, 1278, 1280
HinP1I	GCGC	7 cut(s)	142, 408, 715, 744, 963, 1278, 1280
HincII	GTYRAC	4 cut(s)	367, 1021, 1195, 1291
HindII	GTYRAC	4 cut(s)	367, 1021, 1195, 1291
HpaII	CCGG	7 cut(s)	284, 318, 326, 459, 507, 1098, 1140
HphI	GGTGA	5 cut(s)	212, 448, 691, 1036, 1220
Hpy166II	GTNNAC	9 cut(s)	367, 385, 651, 1021, 1044, 1195, 1291, 1414, 1498
Hpy8I	GTNNAC	9 cut(s)	367, 385, 651, 1021, 1044, 1195, 1291, 1414, 1498
HpyCH4III	ACNGT	6 cut(s)	68, 382, 736, 1018, 1038, 1192
HpyCH4IV	ACGT	8 cut(s)	312, 332, 390, 1011, 1220, 1509, 1551, 1655
HpyF10VI	GCNNNNNNNGC	5 cut(s)	602, 815, 962, 985, 1646
HpyF3I	CTNAG	5 cut(s)	762, 891, 932, 1232, 1484
HpySE526I	ACGT	8 cut(s)	312, 332, 390, 1011, 1220, 1509, 1551, 1655
Hsp92I	GRCGYC	3 cut(s)	363, 956, 1655
Hsp92II	CATG	5 cut(s)	97, 139, 232, 499, 553
HspAI	GCGC	7 cut(s)	142, 408, 715, 744, 963, 1278, 1280
KroI	GCCGGC	1 cut(s)	1139
KroNI	GCCGGC	1 cut(s)	1141
Ksp22I	TGATCA	1 cut(s)	1173
LguI	GCTCTTC	1 cut(s)	1112
LmnI	GCTCC	7 cut(s)	77, 84, 139, 777, 856, 1006, 1488
Lsp1109I	GCAGC	5 cut(s)	126, 699, 886, 911, 1383
MaeI	CTAG	1 cut(s)	1800
MaeII	ACGT	8 cut(s)	312, 332, 390, 1011, 1220, 1509, 1551, 1655
MaeIII	GTNAC	2 cut(s)	16, 1012
MbiI	CCGCTC	3 cut(s)	795, 1357, 1491
MflI	RGATCY	3 cut(s)	538, 935, 990
MhlI	GDGCHC	5 cut(s)	525, 981, 1011, 1233, 1683
MlsI	TGGCCA	1 cut(s)	150
MluCI	AATT	6 cut(s)	208, 620, 643, 659, 812, 1718
MluI	ACGCGT	2 cut(s)	1197, 1458
MluNI	TGGCCA	1 cut(s)	150
MlyI	GAGTC	8 cut(s)	281, 637, 824, 837, 1188, 1419, 1428, 1543
MmeI	TCCRAC	2 cut(s)	333, 871
Mox20I	TGGCCA	1 cut(s)	150
MreI	CGCCGGCG	1 cut(s)	1139
MroNI	GCCGGC	1 cut(s)	1139
MroXI	GAANNNNTTC	2 cut(s)	1736, 1813
MscI	TGGCCA	1 cut(s)	150
MseI	TTAA	1 cut(s)	1825
MslI	CAYNNNNRTG	3 cut(s)	233, 518, 704
Msp20I	TGGCCA	1 cut(s)	150
MspI	CCGG	7 cut(s)	284, 318, 326, 459, 507, 1098, 1140
MspR9I	CCNGG	5 cut(s)	318, 326, 1004, 1031, 1099
MvaI	CCWGG	2 cut(s)	1004, 1031
MwoI	GCNNNNNNNGC	5 cut(s)	602, 815, 962, 985, 1646
NaeI	GCCGGC	1 cut(s)	1141
NciI	CCSGG	3 cut(s)	318, 326, 1099
NcoI	CCATGG	2 cut(s)	135, 549
NgoMIV	GCCGGC	1 cut(s)	1139
NlaIII	CATG	5 cut(s)	97, 139, 232, 499, 553
NlaIV	GGNNCC	4 cut(s)	511, 773, 802, 1681
NmeAIII	GCCGAG	1 cut(s)	1142
NmuCI	GTSAC	2 cut(s)	16, 1012
NruI	TCGCGA	2 cut(s)	878, 1184
PaeR7I	CTCGAG	2 cut(s)	980, 1073
PagI	TCATGA	1 cut(s)	495
PauI	GCGCGC	1 cut(s)	1278
PceI	AGGCCT	1 cut(s)	605
PciSI	GCTCTTC	1 cut(s)	1112
PcsI	WCGNNNNNNNCGW	4 cut(s)	306, 875, 1017, 1506
PdiI	GCCGGC	1 cut(s)	1141
PdmI	GAANNNNTTC	2 cut(s)	1736, 1813
PfeI	GAWTC	3 cut(s)	297, 866, 1732
PflFI	GACNNNGTC	1 cut(s)	1657
PfoI	TCCNGGA	2 cut(s)	316, 1002
Ple19I	CGATCG	2 cut(s)	635, 873
PleI	GAGTC	8 cut(s)	280, 636, 824, 837, 1187, 1418, 1428, 1542
PmaCI	CACGTG	2 cut(s)	1012, 1221
PmlI	CACGTG	2 cut(s)	1012, 1221
PpsI	GAGTC	8 cut(s)	280, 636, 824, 837, 1187, 1418, 1428, 1542
Ppu21I	YACGTR	2 cut(s)	1012, 1221
PpuMI	RGGWCCY	1 cut(s)	559
PshAI	GACNNNNGTC	1 cut(s)	947
Psp124BI	GAGCTC	2 cut(s)	981, 1233
Psp5II	RGGWCCY	1 cut(s)	559
Psp6I	CCWGG	2 cut(s)	1002, 1029
PspCI	CACGTG	2 cut(s)	1012, 1221
PspGI	CCWGG	2 cut(s)	1002, 1029
PspN4I	GGNNCC	4 cut(s)	511, 773, 802, 1681
PspOMI	GGGCCC	1 cut(s)	1679
PspPI	GGNCC	9 cut(s)	286, 323, 559, 800, 1000, 1330, 1618, 1679, 1680
PspPPI	RGGWCCY	1 cut(s)	559
PstI	CTGCAG	2 cut(s)	1626, 1642
PsuI	RGATCY	3 cut(s)	538, 935, 990
PsyI	GACNNNGTC	1 cut(s)	1657
PteI	GCGCGC	1 cut(s)	1278
PvuI	CGATCG	2 cut(s)	635, 873
RruI	TCGCGA	2 cut(s)	878, 1184
RsaI	GTAC	6 cut(s)	384, 440, 573, 1035, 1452, 1610
RsaNI	GTAC	6 cut(s)	383, 439, 572, 1034, 1451, 1609
RseI	CAYNNNNRTG	3 cut(s)	233, 518, 704
SacI	GAGCTC	2 cut(s)	981, 1233
SalI	GTCGAC	3 cut(s)	1019, 1193, 1289
SapI	GCTCTTC	1 cut(s)	1112
SaqAI	TTAA	1 cut(s)	1825
Sau96I	GGNCC	9 cut(s)	286, 323, 559, 800, 1000, 1330, 1618, 1679, 1680
ScaI	AGTACT	1 cut(s)	440
SchI	GAGTC	8 cut(s)	281, 637, 824, 837, 1188, 1419, 1428, 1543
ScrFI	CCNGG	5 cut(s)	318, 326, 1004, 1031, 1099
SduI	GDGCHC	5 cut(s)	525, 981, 1011, 1233, 1683
SfcI	CTRYAG	2 cut(s)	1622, 1638
Sfr274I	CTCGAG	2 cut(s)	980, 1073
SgrAI	CRCCGGYG	1 cut(s)	1139
SgrDI	CGTCGACG	1 cut(s)	1019
SinI	GGWCC	5 cut(s)	286, 559, 800, 1000, 1618
SlaI	CTCGAG	2 cut(s)	980, 1073
SmiMI	CAYNNNNRTG	3 cut(s)	233, 518, 704
SmlI	CTYRAG	5 cut(s)	165, 483, 524, 980, 1073
SmoI	CTYRAG	5 cut(s)	165, 483, 524, 980, 1073
Sse9I	AATT	6 cut(s)	208, 620, 643, 659, 812, 1718
SseBI	AGGCCT	1 cut(s)	605
SspMI	CTAG	1 cut(s)	1800
SstI	GAGCTC	2 cut(s)	981, 1233
StuI	AGGCCT	1 cut(s)	605
StyD4I	CCNGG	5 cut(s)	316, 324, 1002, 1029, 1097
StyI	CCWWGG	3 cut(s)	135, 549, 600
TaaI	ACNGT	6 cut(s)	68, 382, 736, 1018, 1038, 1192
TaiI	ACGT	8 cut(s)	315, 335, 393, 1014, 1223, 1512, 1554, 1658
TaqII	GACCGA	2 cut(s)	988, 989
TasI	AATT	6 cut(s)	208, 620, 643, 659, 812, 1718
TatI	WGTACW	3 cut(s)	382, 438, 1608
TauI	GCSGC	8 cut(s)	347, 398, 413, 465, 596, 720, 809, 821
TfiI	GAWTC	3 cut(s)	297, 866, 1732
Tru1I	TTAA	1 cut(s)	1825
Tru9I	TTAA	1 cut(s)	1825
TscAI	CASTG	2 cut(s)	151, 472
TseFI	GTSAC	2 cut(s)	16, 1012
TseI	GCWGC	5 cut(s)	114, 712, 899, 924, 1371
Tsp45I	GTSAC	2 cut(s)	16, 1012
TspDTI	ATGAA	3 cut(s)	217, 722, 1616
TspGWI	ACGGA	3 cut(s)	392, 1601, 1631
TspRI	CASTG	2 cut(s)	151, 472
Tth111I	GACNNNGTC	1 cut(s)	1657
VpaK11BI	GGWCC	5 cut(s)	286, 559, 800, 1000, 1618
XapI	RAATTY	1 cut(s)	620
XhoI	CTCGAG	2 cut(s)	980, 1073
XmiI	GTMKAC	3 cut(s)	1020, 1194, 1290
XmnI	GAANNNNTTC	2 cut(s)	1736, 1813
XspI	CTAG	1 cut(s)	1800
ZraI	GACGTC	1 cut(s)	1656
ZrmI	AGTACT	1 cut(s)	440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.