FvH4_3g44550

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003838
Physical Location & Seq
Forward (+)
37114298 .. 37118502
4205 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44550.t2

Sequence Viewer

Length: 1209 bp
ATGGACGACAAGTCGGATTCCGGTCAAAAGGCGTCGCTGCTGGGAAGCGGTGGACGTAGTCGGCGGCTTAGTCGGAGAAACTCGTACAACACCCTCGTAAACGACTTCGTTTCGAGGCTGCCGAATAAGGTCCGAGCTGGTCTTGACCCCGAGTCGCCAGACGTCATCAACTGCTCACGTGCCTCCGGATTAACCGAAGGAGAGAAGGAATACTATGAACAGCAATTTGCAACCCTTAAATCCTTTGAAGAAGTTGACGCTATAGTTTCCTCTGACTCCATTGATGAGGAAGATCTTGCTGAACAATTACAACATGAAAGAGCAATGAAGATTTCCAACTATGCAAATATAGTTCTCTTGGCGCTTAAGATCTATGCTACTGTAAAAAGTGGGTCCATAGCCATTGCTGCTTCAACACTAGATTCGTTACTAGATCTCATGGCGGGTGGCATACTCTGGTTTACTCACCTGGCAATGAAGAACGTTAACATCTACCACTATCCTATTGGAAAATTGAGGGTGCAGCCTGTTGGTATAATCATCTTTGCTGCTGTCATGGCTACACTTGGGTTTCAGATATTGATCCAGGCCTTAGAACAGTTGATTACAGCTGATCCCACTGAACAGATGACCTCAGATCAATTGGCGTGGTTGTATGGAATCATGTTGTTTGCCACTGTGGTAAAACTTGCACTGTGGCTCTATTGCAGAACCTCAGGAAATAAGATTGTCCGTGCCTATGCAGAGGATCATTATTTTGATGTGGTAACAAATGTAGTGGGGTTAGTTGCTGCTGTACTTGCTGATAAGTATTATTGGTGGATTGATCCTAGTGGTGCTATTATCCTTGCTCTATACACGATCATAAACTGGTCCAAAACTGTCATGGAAAATGCAGTTTCGCTTGTGGGACAATCTGCCCCTCCTGAATTCCTACAAATGTTGACATATGTTGTCATAAGGCACCCTAAAGTGAAGCGTATTGACACAGTTCGTGCTTACACATTTGGTGTTCTTTATTTTGTGGAGGTTGACATTGAACTCCCAGAGGATTTGCCCTTGAAAGAAGCACATTCCATTGGAGAGACCCTCCAGATAAAACTTGAGAAGCTTCCAGAAGTTGAGCGAGCATTTGTTCATCTCGACTTTGAATGCGACCACAAACCAGAGCACAACGTACTCGTCAAGCTTCCAAACAGTCAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

403

Amino Acids

44.99

Weight (kDa)

5.29

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 2 - 195 1.8e-27 Cation efflux transmembrane domain
ZT_dimer PF16916 200 - 274 5.4e-08 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 165
AccB1I GGYRCC 1 cut(s) 963
AccIII TCCGGA 1 cut(s) 185
AciI CCGC 3 cut(s) 48, 64, 443
AclI AACGTT 1 cut(s) 483
AclWI GGATC 4 cut(s) 577, 608, 756, 821
AcsI RAATTY 1 cut(s) 929
AcvI CACGTG 1 cut(s) 179
AcyI GRCGYC 2 cut(s) 32, 162
AfaI GTAC 3 cut(s) 86, 798, 1179
AflII CTTAAG 1 cut(s) 365
AgsI TTSAA 5 cut(s) 248, 414, 1040, 1063, 1151
AhdI GACNNNNNGTC 2 cut(s) 10, 151
AjnI CCWGG 2 cut(s) 468, 585
AluBI AGCT 4 cut(s) 137, 611, 1111, 1189
AluI AGCT 4 cut(s) 137, 611, 1111, 1189
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 1 cut(s) 1079
AlwI GGATC 4 cut(s) 577, 608, 756, 821
Ama87I CYCGRG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 185
AoxI GGCC 1 cut(s) 588
ApeKI GCWGC 6 cut(s) 37, 118, 407, 523, 548, 791
ApoI RAATTY 1 cut(s) 929
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 3 cut(s) 130, 393, 873
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 3 cut(s) 130, 393, 873
AxyI CCTNAGG 1 cut(s) 715
BanI GGYRCC 1 cut(s) 963
BbrPI CACGTG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 1173
BbvI GCAGC 6 cut(s) 24, 105, 394, 535, 535, 778
BciT130I CCWGG 2 cut(s) 470, 587
BcoDI GTCTC 1 cut(s) 1079
BfaI CTAG 3 cut(s) 419, 431, 831
BfmI CTRYAG 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 365
BfrI CTTAAG 1 cut(s) 365
BglII AGATCT 3 cut(s) 292, 369, 433
BisI GCNGC 7 cut(s) 38, 65, 119, 408, 524, 549, 792
BlsI GCNGC 7 cut(s) 39, 66, 120, 409, 525, 550, 793
Bme1390I CCNGG 2 cut(s) 470, 587
Bme18I GGWCC 3 cut(s) 130, 393, 873
BmeRI GACNNNNNGTC 2 cut(s) 10, 151
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 3 cut(s) 130, 393, 873
BmiI GGNNCC 2 cut(s) 394, 965
BmrFI CCNGG 2 cut(s) 470, 587
BplI GAGNNNNNCTC 2 cut(s) 1074, 1106
BpmI CTGGAG 1 cut(s) 1076
BpuEI CTTGAG 1 cut(s) 1124
BsaAI YACGTR 1 cut(s) 179
BsaBI GATNNNNATC 1 cut(s) 581
BsaHI GRCGYC 2 cut(s) 32, 162
BsaI GGTCTC 1 cut(s) 1079
BsaWI WCCGGW 2 cut(s) 20, 185
Bse1I ACTGG 1 cut(s) 875
Bse21I CCTNAGG 1 cut(s) 715
Bse3DI GCAATG 3 cut(s) 330, 402, 480
Bse8I GATNNNNATC 1 cut(s) 581
BseAI TCCGGA 1 cut(s) 185
BseBI CCWGG 2 cut(s) 470, 587
BseJI GATNNNNATC 1 cut(s) 581
BseMI GCAATG 3 cut(s) 330, 402, 480
BseMII CTCAG 2 cut(s) 648, 729
BseNI ACTGG 1 cut(s) 875
BseXI GCAGC 6 cut(s) 24, 105, 394, 535, 535, 778
BseYI CCCAGC 1 cut(s) 40
BsgI GTGCAG 1 cut(s) 542
BshFI GGCC 1 cut(s) 590
BshNI GGYRCC 1 cut(s) 963
BsiHKAI GWGCWC 1 cut(s) 1173
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 2 cut(s) 21, 186
BslFI GGGAC 1 cut(s) 924
BsmAI GTCTC 1 cut(s) 1079
BsmFI GGGAC 1 cut(s) 924
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 1 cut(s) 590
Bso31I GGTCTC 1 cut(s) 1079
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 1173
Bsp13I TCCGGA 1 cut(s) 185
Bsp143I GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
BspACI CCGC 3 cut(s) 48, 64, 443
BspANI GGCC 1 cut(s) 590
BspCNI CTCAG 2 cut(s) 647, 728
BspEI TCCGGA 1 cut(s) 185
BspLI GGNNCC 2 cut(s) 394, 965
BspPI GGATC 4 cut(s) 577, 608, 756, 821
BspT107I GGYRCC 1 cut(s) 963
BspTI CTTAAG 1 cut(s) 365
BspTNI GGTCTC 1 cut(s) 1079
BsrDI GCAATG 3 cut(s) 330, 402, 480
BsrI ACTGG 1 cut(s) 875
BssMI GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
BssNI GRCGYC 2 cut(s) 32, 162
Bst2UI CCWGG 2 cut(s) 470, 587
Bst4CI ACNGT 7 cut(s) 382, 600, 679, 696, 883, 991, 1199
BstACI GRCGYC 2 cut(s) 32, 162
BstAFI CTTAAG 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 1128
BstDEI CTNAG 4 cut(s) 68, 592, 634, 715
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 9 cut(s) 295, 372, 436, 585, 616, 640, 751, 829, 864
BstMAI GTCTC 1 cut(s) 1079
BstMBI GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
BstMWI GCNNNNNNNGC 3 cut(s) 407, 557, 800
BstNI CCWGG 2 cut(s) 470, 587
BstSCI CCNGG 2 cut(s) 468, 585
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 6 cut(s) 24, 105, 394, 535, 535, 778
BstX2I RGATCY 3 cut(s) 292, 369, 433
BstYI RGATCY 3 cut(s) 292, 369, 433
Bsu36I CCTNAGG 1 cut(s) 715
BsuRI GGCC 1 cut(s) 590
BtsIMutI CAGTG 3 cut(s) 618, 675, 692
Cac8I GCNNGC 1 cut(s) 1128
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 3 cut(s) 130, 393, 873
CseI GACGC 2 cut(s) 21, 266
Csp6I GTAC 3 cut(s) 85, 797, 1178
CspCI CAANNNNNGTGG 4 cut(s) 629, 664, 759, 794
CviAII CATG 5 cut(s) 314, 439, 556, 664, 886
CviQI GTAC 3 cut(s) 85, 797, 1178
DdeI CTNAG 4 cut(s) 68, 592, 634, 715
DpnI GATC 9 cut(s) 294, 371, 435, 584, 615, 639, 750, 828, 863
DpnII GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
DriI GACNNNNNGTC 2 cut(s) 10, 151
Eam1105I GACNNNNNGTC 2 cut(s) 10, 151
Eco147I AGGCCT 1 cut(s) 590
Eco31I GGTCTC 1 cut(s) 1079
Eco47I GGWCC 3 cut(s) 130, 393, 873
Eco72I CACGTG 1 cut(s) 179
Eco81I CCTNAGG 1 cut(s) 715
Eco88I CYCGRG 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 2 cut(s) 468, 585
FaeI CATG 5 cut(s) 317, 442, 559, 667, 889
FaqI GGGAC 1 cut(s) 924
FatI CATG 5 cut(s) 313, 438, 555, 663, 885
FauI CCCGC 1 cut(s) 436
FauNDI CATATG 1 cut(s) 949
Fnu4HI GCNGC 7 cut(s) 38, 65, 119, 408, 524, 549, 792
Fsp4HI GCNGC 7 cut(s) 38, 65, 119, 408, 524, 549, 792
FspBI CTAG 3 cut(s) 419, 431, 831
GlaI GCGC 1 cut(s) 363
GluI GCNGC 7 cut(s) 38, 65, 119, 408, 524, 549, 792
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 1076
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 1 cut(s) 590
HapII CCGG 2 cut(s) 21, 186
HgaI GACGC 2 cut(s) 21, 266
HhaI GCGC 1 cut(s) 364
Hin1I GRCGYC 2 cut(s) 32, 162
Hin1II CATG 5 cut(s) 317, 442, 559, 667, 889
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 4 cut(s) 256, 487, 945, 1033
HindII GTYRAC 4 cut(s) 256, 487, 945, 1033
HindIII AAGCTT 2 cut(s) 1109, 1187
HinfI GANTC 5 cut(s) 17, 152, 275, 422, 660
HpaI GTTAAC 1 cut(s) 487
HpaII CCGG 2 cut(s) 21, 186
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 7 cut(s) 53, 100, 256, 462, 487, 945, 1033
Hpy188I TCNGA 6 cut(s) 16, 75, 134, 274, 576, 637
Hpy188III TCNNGA 7 cut(s) 143, 186, 717, 926, 1093, 1115, 1142
Hpy8I GTNNAC 7 cut(s) 53, 100, 256, 462, 487, 945, 1033
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 2 cut(s) 191, 199
HpyCH4III ACNGT 7 cut(s) 382, 600, 679, 696, 883, 991, 1199
HpyCH4IV ACGT 5 cut(s) 55, 162, 178, 483, 1176
HpyCH4V TGCA 7 cut(s) 230, 344, 523, 692, 708, 743, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 557, 800
HpyF3I CTNAG 4 cut(s) 68, 592, 634, 715
HpySE526I ACGT 5 cut(s) 55, 162, 178, 483, 1176
Hsp92I GRCGYC 2 cut(s) 32, 162
Hsp92II CATG 5 cut(s) 317, 442, 559, 667, 889
HspAI GCGC 1 cut(s) 362
Kpn2I TCCGGA 1 cut(s) 185
KspAI GTTAAC 1 cut(s) 487
Kzo9I GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
Lsp1109I GCAGC 6 cut(s) 24, 105, 394, 535, 535, 778
MaeI CTAG 3 cut(s) 419, 431, 831
MaeII ACGT 5 cut(s) 55, 162, 178, 483, 1176
MaeIII GTNAC 2 cut(s) 426, 766
MalI GATC 9 cut(s) 294, 371, 435, 584, 615, 639, 750, 828, 863
MboI GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
MboII GAAGA 4 cut(s) 260, 302, 340, 490
MfeI CAATTG 1 cut(s) 641
MflI RGATCY 3 cut(s) 292, 369, 433
MhlI GDGCHC 1 cut(s) 1173
MluCI AATT 5 cut(s) 224, 305, 512, 641, 929
MlyI GAGTC 2 cut(s) 161, 269
MmeI TCCRAC 2 cut(s) 53, 360
MroI TCCGGA 1 cut(s) 185
MseI TTAA 4 cut(s) 191, 237, 366, 486
MspA1I CMGCKG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 365
MspI CCGG 2 cut(s) 21, 186
MspR9I CCNGG 2 cut(s) 470, 587
MunI CAATTG 1 cut(s) 641
Mva1269I GAATGC 1 cut(s) 1157
MvaI CCWGG 2 cut(s) 470, 587
MwoI GCNNNNNNNGC 3 cut(s) 407, 557, 800
NdeI CATATG 1 cut(s) 949
NdeII GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
NlaIII CATG 5 cut(s) 317, 442, 559, 667, 889
NlaIV GGNNCC 2 cut(s) 394, 965
PceI AGGCCT 1 cut(s) 590
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 1 cut(s) 1157
PfeI GAWTC 3 cut(s) 17, 422, 660
PflFI GACNNNGTC 1 cut(s) 57
PkrI GCNGC 7 cut(s) 39, 66, 120, 409, 525, 550, 793
PleI GAGTC 2 cut(s) 160, 269
PmaCI CACGTG 1 cut(s) 179
PmlI CACGTG 1 cut(s) 179
PpsI GAGTC 2 cut(s) 160, 269
Ppu21I YACGTR 1 cut(s) 179
Psp1406I AACGTT 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 468, 585
PspCI CACGTG 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 2 cut(s) 468, 585
PspN4I GGNNCC 2 cut(s) 394, 965
PspPI GGNCC 3 cut(s) 130, 393, 873
PsuI RGATCY 3 cut(s) 292, 369, 433
PsyI GACNNNGTC 1 cut(s) 57
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 3 cut(s) 86, 798, 1179
RsaNI GTAC 3 cut(s) 85, 797, 1178
SaqAI TTAA 4 cut(s) 191, 237, 366, 486
SatI GCNGC 7 cut(s) 38, 65, 119, 408, 524, 549, 792
Sau3AI GATC 9 cut(s) 292, 369, 433, 582, 613, 637, 748, 826, 861
Sau96I GGNCC 3 cut(s) 130, 393, 873
SchI GAGTC 2 cut(s) 161, 269
ScrFI CCNGG 2 cut(s) 470, 587
SduI GDGCHC 1 cut(s) 1173
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 3 cut(s) 130, 393, 873
SmlI CTYRAG 2 cut(s) 365, 1103
SmoI CTYRAG 2 cut(s) 365, 1103
Sse9I AATT 5 cut(s) 224, 305, 512, 641, 929
SseBI AGGCCT 1 cut(s) 590
SsiI CCGC 3 cut(s) 48, 64, 443
SspMI CTAG 3 cut(s) 419, 431, 831
StuI AGGCCT 1 cut(s) 590
StyD4I CCNGG 2 cut(s) 468, 585
TaaI ACNGT 7 cut(s) 382, 600, 679, 696, 883, 991, 1199
TaiI ACGT 5 cut(s) 58, 165, 181, 486, 1179
TaqI TCGA 2 cut(s) 113, 1143
TasI AATT 5 cut(s) 224, 305, 512, 641, 929
TatI WGTACW 1 cut(s) 796
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 3 cut(s) 17, 422, 660
Tru1I TTAA 4 cut(s) 191, 237, 366, 486
Tru9I TTAA 4 cut(s) 191, 237, 366, 486
TscAI CASTG 3 cut(s) 625, 682, 699
TseI GCWGC 6 cut(s) 37, 118, 407, 523, 548, 791
TspDTI ATGAA 5 cut(s) 231, 330, 341, 491, 1127
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 3 cut(s) 625, 682, 699
Tth111I GACNNNGTC 1 cut(s) 57
Vha464I CTTAAG 1 cut(s) 365
VpaK11BI GGWCC 3 cut(s) 130, 393, 873
XapI RAATTY 1 cut(s) 929
XcmI CCANNNNNNNNNTGG 2 cut(s) 503, 883
XspI CTAG 3 cut(s) 419, 431, 831
ZraI GACGTC 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.