MD11G1014600.v1.1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003838
Physical Location & Seq
Reverse (-)
1154826 .. 1158377
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1014600.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGAGGAAAAATCGGAAACGGCCGGTGTGAAAACGCCGCTGTTGGGCAGCGGCGGACGCAGCCGGCGACTGAGCCGAAGAAACTCGTACAACTCACTCAGAAGCGACTTCATTTCGAGGCTCCCTGACAAGGTCCGTGCCGGTATCGACCCCGAGTCTCCTGACGACATTGACTTCTCTCGTACCTCCGCCTTGAACCGAGGAGAAAAGGAATACTATGAAGCCCAATTTGCAACACTTAAATCCTTTGAGGAGGTTGACTTCGCGGTGGCAGCCGACTGCGTTGATGAGGACGATTTCGAGGAACAAGCACAACATGAAAGAGCAATGAAGATATCCAACTACGCCAATATAATTCTCTTGGGACTTAAGATCTATGCTACTGTAAAGAGTGGATCGATAGCCATTGCTGCATCGACTCTAGATTCCTTGCTCGATCTCATGGCCGGTGGAATACTTTGGTTCACTCACCTGGCAATGAAGAACATCAATATCTATAAATATCCTATCGGAAAATTGAGGGTGCAGCCTGTCGGTATAATCATCTTTGCTGCTATCATGGCTACACTCGGTTTTCAGGTCTTGATCCAGGCTTTAGAACAACTAATAACCGGTGATGCCACGGAAAAGATGACCTCGGACCAATTGGTGTGGTTGTACGCAATCATGATATTTGCCACCATGGTAAAACTTGCACTCTGGTTATACTGCAGGACCTCAGGGAACAAGATTGTCCGTGCTTACGCAAAGGATCACTATTTTGATGTGGTAACGAACGTAGTAGGATTAGTGGCTGCTGTTCTTGCTGATAAGTTTATGTGGTGGATTGATCCTGCTGGTGCTATTATCCTTGCTATATACACGATTACAAATTGGTCCGGAACTGTTCTAGAAAATGCAGTTTCCCTCGTGGGACAATCAGCGCCTCCGGAATTCTTACAAAAACTGACATACATTACCATAATGCACCCTCAAGTGAAGCGTATTGACACAGTTCGCGCTTACACATTTGGTGTTCTTTATTTCGTAGAGGTTGACATTGAACTCCCGGAGGAGTTACCCTTGAAAGAGGCACATGCCATTGGAGAGACCCTCCAGATAAAGCTCGAGAAACTTCCCGACGTTGAGCGAGCATTTGTTCATCTTGACTTCGAATGTGATCACAAACCGGAGCATTCTGTACTTGTCAAGCTTCCGAACACTCAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

404

Amino Acids

45.12

Weight (kDa)

5.32

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 111 - 304 4.2e-26 Cation efflux transmembrane domain
ZT_dimer PF16916 309 - 383 1.7e-07 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 266, 999
AccIII TCCGGA 2 cut(s) 878, 928
AciI CCGC 5 cut(s) 38, 51, 54, 189, 266
AclWI GGATC 4 cut(s) 403, 580, 759, 824
AcoI YGGCCR 2 cut(s) 21, 444
AcsI RAATTY 1 cut(s) 932
AfaI GTAC 4 cut(s) 89, 184, 659, 1182
AfiI CCNNNNNNNGG 2 cut(s) 44, 130
AflII CTTAAG 1 cut(s) 368
AgeI ACCGGT 1 cut(s) 611
AgsI TTSAA 3 cut(s) 196, 1043, 1066
AhdI GACNNNNNGTC 1 cut(s) 154
AjnI CCWGG 2 cut(s) 471, 588
AluBI AGCT 2 cut(s) 1105, 1192
AluI AGCT 2 cut(s) 1105, 1192
Alw26I GTCTC 2 cut(s) 162, 1082
AlwI GGATC 4 cut(s) 403, 580, 759, 824
Ama87I CYCGRG 2 cut(s) 152, 1106
Aor13HI TCCGGA 2 cut(s) 878, 928
AoxI GGCC 2 cut(s) 21, 444
ApeKI GCWGC 7 cut(s) 48, 60, 272, 410, 526, 551, 794
ApoI RAATTY 1 cut(s) 932
AsiGI ACCGGT 1 cut(s) 611
AspLEI GCGC 2 cut(s) 925, 1001
AspS9I GGNCC 4 cut(s) 133, 640, 714, 876
AsuC2I CCSGG 1 cut(s) 1049
AsuHPI GGTGA 2 cut(s) 461, 626
AsuII TTCGAA 1 cut(s) 1152
AvaI CYCGRG 2 cut(s) 152, 1106
AvaII GGWCC 4 cut(s) 133, 640, 714, 876
AxyI CCTNAGG 1 cut(s) 718
BauI CACGAG 1 cut(s) 908
BbvI GCAGC 7 cut(s) 60, 72, 284, 397, 538, 538, 781
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 2 cut(s) 473, 590
BclI TGATCA 1 cut(s) 1159
BcnI CCSGG 1 cut(s) 1049
BcoDI GTCTC 2 cut(s) 162, 1082
BfaI CTAG 2 cut(s) 422, 890
BfmI CTRYAG 1 cut(s) 709
BfoI RGCGCY 1 cut(s) 926
BfrI CTTAAG 1 cut(s) 368
BglII AGATCT 1 cut(s) 372
BisI GCNGC 9 cut(s) 38, 49, 52, 61, 273, 411, 527, 552, 795
BlsI GCNGC 9 cut(s) 39, 50, 53, 62, 274, 412, 528, 553, 796
Bme1390I CCNGG 3 cut(s) 473, 590, 1049
Bme18I GGWCC 4 cut(s) 133, 640, 714, 876
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 2 cut(s) 152, 1106
BmgT120I GGNCC 4 cut(s) 133, 640, 714, 876
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 3 cut(s) 473, 590, 1049
BmsI GCATC 2 cut(s) 422, 607
BplI GAGNNNNNCTC 2 cut(s) 1077, 1109
BpmI CTGGAG 1 cut(s) 1079
Bpu14I TTCGAA 1 cut(s) 1152
BpuEI CTTGAG 1 cut(s) 957
BpuMI CCSGG 1 cut(s) 1049
Bsa29I ATCGAT 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 1082
BsaJI CCNNGG 4 cut(s) 199, 621, 636, 681
BsaWI WCCGGW 4 cut(s) 611, 878, 928, 1168
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 130
Bse118I RCCGGY 5 cut(s) 23, 63, 140, 446, 611
Bse21I CCTNAGG 1 cut(s) 718
Bse3DI GCAATG 3 cut(s) 333, 405, 483
BseAI TCCGGA 2 cut(s) 878, 928
BseBI CCWGG 2 cut(s) 473, 590
BseCI ATCGAT 1 cut(s) 398
BseDI CCNNGG 4 cut(s) 199, 621, 636, 681
BseLI CCNNNNNNNGG 2 cut(s) 44, 130
BseMI GCAATG 3 cut(s) 333, 405, 483
BseMII CTCAG 3 cut(s) 62, 112, 732
BseRI GAGGAG 3 cut(s) 216, 266, 1067
BseX3I CGGCCG 1 cut(s) 21
BseXI GCAGC 7 cut(s) 60, 72, 284, 397, 538, 538, 781
BsgI GTGCAG 1 cut(s) 545
Bsh1236I CGCG 2 cut(s) 266, 999
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 23, 446
BshTI ACCGGT 1 cut(s) 611
BshVI ATCGAT 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 24
BsiHKCI CYCGRG 2 cut(s) 152, 1106
BsiSI CCGG 9 cut(s) 24, 64, 141, 447, 612, 879, 929, 1049, 1169
BslFI GGGAC 2 cut(s) 378, 927
BslI CCNNNNNNNGG 2 cut(s) 44, 130
BsmAI GTCTC 2 cut(s) 162, 1082
BsmFI GGGAC 2 cut(s) 378, 927
BsmI GAATGC 1 cut(s) 1174
BsnI GGCC 2 cut(s) 23, 446
Bso31I GGTCTC 1 cut(s) 1082
BsoBI CYCGRG 2 cut(s) 152, 1106
Bsp119I TTCGAA 1 cut(s) 1152
Bsp13I TCCGGA 2 cut(s) 878, 928
Bsp143I GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
Bsp19I CCATGG 1 cut(s) 681
BspACI CCGC 5 cut(s) 38, 51, 54, 189, 266
BspANI GGCC 2 cut(s) 23, 446
BspCNI CTCAG 3 cut(s) 63, 111, 731
BspDI ATCGAT 1 cut(s) 398
BspEI TCCGGA 2 cut(s) 878, 928
BspFNI CGCG 2 cut(s) 266, 999
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 1 cut(s) 122
BspMAI CTGCAG 1 cut(s) 713
BspPI GGATC 4 cut(s) 403, 580, 759, 824
BspT104I TTCGAA 1 cut(s) 1152
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 1082
BsrDI GCAATG 3 cut(s) 333, 405, 483
BsrFI RCCGGY 5 cut(s) 23, 63, 140, 446, 611
BssAI RCCGGY 5 cut(s) 23, 63, 140, 446, 611
BssECI CCNNGG 4 cut(s) 199, 621, 636, 681
BssMI GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
BssSI CACGAG 1 cut(s) 908
BssT1I CCWWGG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 908
Bst2UI CCWGG 2 cut(s) 473, 590
Bst4CI ACNGT 3 cut(s) 385, 886, 994
BstAFI CTTAAG 1 cut(s) 368
BstBI TTCGAA 1 cut(s) 1152
BstC8I GCNNGC 2 cut(s) 65, 1131
BstDEI CTNAG 4 cut(s) 71, 98, 718, 1203
BstDSI CCRYGG 2 cut(s) 621, 681
BstFNI CGCG 2 cut(s) 266, 999
BstH2I RGCGCY 1 cut(s) 926
BstHHI GCGC 2 cut(s) 925, 1001
BstKTI GATC 7 cut(s) 375, 398, 439, 588, 754, 832, 1162
BstMAI GTCTC 2 cut(s) 162, 1082
BstMBI GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 7 cut(s) 57, 60, 230, 272, 410, 560, 803
BstNI CCWGG 2 cut(s) 473, 590
BstNSI RCATGY 1 cut(s) 1079
BstSCI CCNGG 3 cut(s) 471, 588, 1047
BstSFI CTRYAG 1 cut(s) 709
BstUI CGCG 2 cut(s) 266, 999
BstV1I GCAGC 7 cut(s) 60, 72, 284, 397, 538, 538, 781
BstX2I RGATCY 1 cut(s) 372
BstYI RGATCY 1 cut(s) 372
BstZI CGGCCG 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 718
BsuRI GGCC 2 cut(s) 23, 446
BsuTUI ATCGAT 1 cut(s) 398
BtgI CCRYGG 2 cut(s) 621, 681
Cac8I GCNNGC 2 cut(s) 65, 1131
CciI TCATGA 1 cut(s) 666
CfoI GCGC 2 cut(s) 925, 1001
Cfr10I RCCGGY 5 cut(s) 23, 63, 140, 446, 611
Cfr13I GGNCC 4 cut(s) 133, 640, 714, 876
ClaI ATCGAT 1 cut(s) 398
CseI GACGC 1 cut(s) 66
Csp6I GTAC 4 cut(s) 88, 183, 658, 1181
CspAI ACCGGT 1 cut(s) 611
CspCI CAANNNNNGTGG 2 cut(s) 632, 667
CviAII CATG 6 cut(s) 317, 442, 559, 667, 682, 1076
CviQI GTAC 4 cut(s) 88, 183, 658, 1181
DdeI CTNAG 4 cut(s) 71, 98, 718, 1203
DpnI GATC 7 cut(s) 374, 397, 438, 587, 753, 831, 1161
DpnII GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
DriI GACNNNNNGTC 1 cut(s) 154
EaeI YGGCCR 2 cut(s) 21, 444
EagI CGGCCG 1 cut(s) 21
Eam1105I GACNNNNNGTC 1 cut(s) 154
EciI GGCGGA 2 cut(s) 69, 178
EclXI CGGCCG 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 681
Eco31I GGTCTC 1 cut(s) 1082
Eco32I GATATC 1 cut(s) 336
Eco47I GGWCC 4 cut(s) 133, 640, 714, 876
Eco52I CGGCCG 1 cut(s) 21
Eco81I CCTNAGG 1 cut(s) 718
Eco88I CYCGRG 2 cut(s) 152, 1106
EcoO109I RGGNCCY 1 cut(s) 714
EcoRI GAATTC 1 cut(s) 932
EcoRII CCWGG 2 cut(s) 471, 588
EcoRV GATATC 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 6 cut(s) 320, 445, 562, 670, 685, 1079
FaqI GGGAC 2 cut(s) 378, 927
FatI CATG 6 cut(s) 316, 441, 558, 666, 681, 1075
FbaI TGATCA 1 cut(s) 1159
Fnu4HI GCNGC 9 cut(s) 38, 49, 52, 61, 273, 411, 527, 552, 795
Fsp4HI GCNGC 9 cut(s) 38, 49, 52, 61, 273, 411, 527, 552, 795
FspBI CTAG 2 cut(s) 422, 890
GlaI GCGC 2 cut(s) 924, 1000
GluI GCNGC 9 cut(s) 38, 49, 52, 61, 273, 411, 527, 552, 795
GsuI CTGGAG 1 cut(s) 1079
HaeII RGCGCY 1 cut(s) 926
HaeIII GGCC 2 cut(s) 23, 446
HapII CCGG 9 cut(s) 24, 64, 141, 447, 612, 879, 929, 1049, 1169
HgaI GACGC 1 cut(s) 66
HhaI GCGC 2 cut(s) 925, 1001
Hin1II CATG 6 cut(s) 320, 445, 562, 670, 685, 1079
Hin6I GCGC 2 cut(s) 923, 999
HinP1I GCGC 2 cut(s) 923, 999
HincII GTYRAC 2 cut(s) 259, 1036
HindII GTYRAC 2 cut(s) 259, 1036
HindIII AAGCTT 1 cut(s) 1190
HinfI GANTC 3 cut(s) 155, 418, 425
HpaII CCGG 9 cut(s) 24, 64, 141, 447, 612, 879, 929, 1049, 1169
HphI GGTGA 2 cut(s) 461, 626
Hpy166II GTNNAC 3 cut(s) 259, 465, 1036
Hpy188I TCNGA 5 cut(s) 16, 101, 512, 640, 1197
Hpy8I GTNNAC 3 cut(s) 259, 465, 1036
Hpy99I CGWCG 1 cut(s) 1124
HpyCH4III ACNGT 3 cut(s) 385, 886, 994
HpyCH4IV ACGT 2 cut(s) 777, 1122
HpyCH4V TGCA 7 cut(s) 233, 413, 526, 695, 711, 899, 967
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 60, 230, 272, 410, 560, 803
HpyF3I CTNAG 4 cut(s) 71, 98, 718, 1203
HpySE526I ACGT 2 cut(s) 777, 1122
Hsp92II CATG 6 cut(s) 320, 445, 562, 670, 685, 1079
HspAI GCGC 2 cut(s) 923, 999
Kpn2I TCCGGA 2 cut(s) 878, 928
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 1159
Kzo9I GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
LmnI GCTCC 2 cut(s) 126, 1171
Lsp1109I GCAGC 7 cut(s) 60, 72, 284, 397, 538, 538, 781
LweI GCATC 2 cut(s) 422, 607
MaeI CTAG 2 cut(s) 422, 890
MaeII ACGT 2 cut(s) 777, 1122
MaeIII GTNAC 2 cut(s) 769, 1056
MalI GATC 7 cut(s) 374, 397, 438, 587, 753, 831, 1161
MboI GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
MboII GAAGA 3 cut(s) 90, 343, 493
MfeI CAATTG 1 cut(s) 644
MflI RGATCY 1 cut(s) 372
MluCI AATT 6 cut(s) 227, 354, 515, 644, 871, 932
MlyI GAGTC 2 cut(s) 164, 412
MmeI TCCRAC 1 cut(s) 363
MroI TCCGGA 2 cut(s) 878, 928
MroNI GCCGGC 1 cut(s) 63
MseI TTAA 2 cut(s) 240, 369
MspA1I CMGCKG 2 cut(s) 40, 51
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 9 cut(s) 24, 64, 141, 447, 612, 879, 929, 1049, 1169
MspR9I CCNGG 3 cut(s) 473, 590, 1049
MunI CAATTG 1 cut(s) 644
Mva1269I GAATGC 1 cut(s) 1174
MvaI CCWGG 2 cut(s) 473, 590
MvnI CGCG 2 cut(s) 266, 999
MwoI GCNNNNNNNGC 7 cut(s) 57, 60, 230, 272, 410, 560, 803
NaeI GCCGGC 1 cut(s) 65
NciI CCSGG 1 cut(s) 1049
NcoI CCATGG 1 cut(s) 681
NdeII GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
NgoMIV GCCGGC 1 cut(s) 63
NlaIII CATG 6 cut(s) 320, 445, 562, 670, 685, 1079
NlaIV GGNNCC 1 cut(s) 122
NspI RCATGY 1 cut(s) 1079
NspV TTCGAA 1 cut(s) 1152
PaeR7I CTCGAG 1 cut(s) 1106
PagI TCATGA 1 cut(s) 666
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PctI GAATGC 1 cut(s) 1174
PdiI GCCGGC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 425
PflFI GACNNNGTC 1 cut(s) 131
PfoI TCCNGGA 1 cut(s) 1047
PinAI ACCGGT 1 cut(s) 611
PkrI GCNGC 9 cut(s) 39, 50, 53, 62, 274, 412, 528, 553, 796
PleI GAGTC 2 cut(s) 163, 412
PpsI GAGTC 2 cut(s) 163, 412
PpuMI RGGWCCY 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 471, 588
PspGI CCWGG 2 cut(s) 471, 588
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 4 cut(s) 133, 640, 714, 876
PspPPI RGGWCCY 1 cut(s) 714
PstI CTGCAG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 372
PsyI GACNNNGTC 1 cut(s) 131
RsaI GTAC 4 cut(s) 89, 184, 659, 1182
RsaNI GTAC 4 cut(s) 88, 183, 658, 1181
SaqAI TTAA 2 cut(s) 240, 369
SatI GCNGC 9 cut(s) 38, 49, 52, 61, 273, 411, 527, 552, 795
Sau3AI GATC 7 cut(s) 372, 395, 436, 585, 751, 829, 1159
Sau96I GGNCC 4 cut(s) 133, 640, 714, 876
SchI GAGTC 2 cut(s) 164, 412
ScrFI CCNGG 3 cut(s) 473, 590, 1049
SfaNI GCATC 2 cut(s) 422, 607
SfcI CTRYAG 1 cut(s) 709
Sfr274I CTCGAG 1 cut(s) 1106
SfuI TTCGAA 1 cut(s) 1152
SinI GGWCC 4 cut(s) 133, 640, 714, 876
SlaI CTCGAG 1 cut(s) 1106
SmlI CTYRAG 3 cut(s) 368, 972, 1106
SmoI CTYRAG 3 cut(s) 368, 972, 1106
Sse9I AATT 6 cut(s) 227, 354, 515, 644, 871, 932
SsiI CCGC 5 cut(s) 38, 51, 54, 189, 266
SspMI CTAG 2 cut(s) 422, 890
StyD4I CCNGG 3 cut(s) 471, 588, 1047
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 3 cut(s) 385, 886, 994
TaiI ACGT 2 cut(s) 780, 1125
TaqI TCGA 8 cut(s) 116, 147, 300, 398, 416, 435, 1107, 1152
TasI AATT 6 cut(s) 227, 354, 515, 644, 871, 932
TatI WGTACW 1 cut(s) 1180
TauI GCSGC 2 cut(s) 40, 54
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 2 cut(s) 240, 369
Tru9I TTAA 2 cut(s) 240, 369
TseI GCWGC 7 cut(s) 48, 60, 272, 410, 526, 551, 794
TspDTI ATGAA 6 cut(s) 100, 234, 333, 344, 494, 1130
TspGWI ACGGA 3 cut(s) 125, 638, 725
Tth111I GACNNNGTC 1 cut(s) 131
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 4 cut(s) 133, 640, 714, 876
XapI RAATTY 1 cut(s) 932
XbaI TCTAGA 2 cut(s) 421, 889
XceI RCATGY 1 cut(s) 1079
XhoI CTCGAG 1 cut(s) 1106
XspI CTAG 2 cut(s) 422, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.