Rh5DG561100

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003838
Physical Location & Seq
Forward (+)
86558577 .. 86565302
6726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG561100.1

Sequence Viewer

Length: 1209 bp
ATGGAGGACAAGTCGGAATCGGGTCTGAAGGCGTCGCTGTTGGGAAGCGGTGGACGTAGTCGGCGACTGAGTCGGAAAAACTCGTACAGCACCCTCGTAAACGACTTCATTTCGAGGCTGCCACCGAAAGTCCGAGCAGGTGTTGACCCCGAGTCTCGGGAAGTGATCGACTGCTCACGTGCCTCGGGATTAAGCGAAGGAGAGAAGGAATACTATGAAAAACAATTTGCAACCCTTAAATCCTTTGAGGAAGTTGACACTGTAGTCGCCGCTGAGGGCATTGATGAGGAAAATCTTGCAGAACAAGCACAACATGAAAGAGCAATGAAGATTTCCAACTATGCAAATATAATTCTGTTGGCACTTAAGATTTATGCTACTGTAAAAAGTGGGTCCATAGCCATTGCTGCTTCAACACTAGATTCTTTACTAGATCTCATGGCGGGTGGCATACTCTGGTTCACTCACCTGGCAATGAAGAACGTTAATATCTACCACTATCCTATTGGAAAATTGAGGGTGCAGCCTGTTGGCATAATCATCTTTGCTGCTGTCATGGCTACACTTGGGTTTCAGGTATTGATCCAGGCCGTAGAACAATTAATTACAGCTGATCCCACTGAAAGTCTGACCTCAGATCAATTGGCGTGGTTGTATGGAATCATGTTGTTTGCTACTGTGGTAAAACTTGCACTGTGGCTCTATTGCAGAACCTCAGGAAATAAGATTGTCCGTGCCTTTGCAGAGGATCACTATTTTGATGTGGTAACTAATGTAGTGGGATTAGTTGCTGCTGTACTTGCTGATAAGTATTACTGGTGGATTGATCCTACTGGTGCTATTATCCTTGCTCTGTATACGATCATAAATTGGTCCAAAACTGTCATGGAAAATGCAGTTTCGCTGGTGGGACAGTCAGCCCCTCCTGAATTCCTACAAATGTTGACATATGTTGTCATAAGGCACCCTCAAGTGAAGCGTGTTGACACAGTTCGTGCTTACACATTTGGTGTTCTTTATTTTGTGGAGGTTGACATTGAACTCCCAGAAGAATTACCCTTGAAAGAAGCACATGCCATTGGAGAGTCCCTCCAGATAAATCTTGAGAAGCTTCCAGAAGTTGAGCGAGCATTTGTTCATCTTGACTTTGAATGCGACCACAAACCAGAGCACAATGTACTCGTCAAGCTTCCAAGCAGTCAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

402

Amino Acids

44.77

Weight (kDa)

5.32

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 110 - 303 1.1e-26 Cation efflux transmembrane domain
ZT_dimer PF16916 308 - 382 8.2e-08 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
AasI GACNNNNNNGTC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 963
AccI GTMKAC 1 cut(s) 857
AciI CCGC 3 cut(s) 48, 270, 443
AclI AACGTT 1 cut(s) 483
AclWI GGATC 4 cut(s) 577, 608, 756, 821
AcsI RAATTY 1 cut(s) 929
AcuI CTGAAG 1 cut(s) 47
AcvI CACGTG 1 cut(s) 179
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 3 cut(s) 86, 798, 1179
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 365
AgsI TTSAA 4 cut(s) 414, 1040, 1063, 1151
AhdI GACNNNNNGTC 1 cut(s) 151
AjnI CCWGG 2 cut(s) 468, 585
AluBI AGCT 3 cut(s) 611, 1111, 1189
AluI AGCT 3 cut(s) 611, 1111, 1189
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 4 cut(s) 577, 608, 756, 821
Ama87I CYCGRG 3 cut(s) 149, 156, 184
AoxI GGCC 1 cut(s) 588
ApeKI GCWGC 5 cut(s) 118, 407, 523, 548, 791
ApoI RAATTY 1 cut(s) 929
AseI ATTAAT 1 cut(s) 602
AspS9I GGNCC 2 cut(s) 393, 873
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 3 cut(s) 149, 156, 184
AvaII GGWCC 2 cut(s) 393, 873
AxyI CCTNAGG 1 cut(s) 715
BanI GGYRCC 1 cut(s) 963
BbrPI CACGTG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 1173
BbvCI CCTCAGC 1 cut(s) 273
BbvI GCAGC 5 cut(s) 105, 394, 535, 535, 778
BceAI ACGGC 1 cut(s) 575
BciT130I CCWGG 2 cut(s) 470, 587
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 2 cut(s) 419, 431
BfmI CTRYAG 1 cut(s) 261
BfrI CTTAAG 1 cut(s) 365
BfuAI ACCTGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 433
BisI GCNGC 6 cut(s) 119, 270, 408, 524, 549, 792
BlsI GCNGC 6 cut(s) 120, 271, 409, 525, 550, 793
Bme1390I CCNGG 2 cut(s) 470, 587
Bme18I GGWCC 2 cut(s) 393, 873
BmeRI GACNNNNNGTC 1 cut(s) 151
BmeT110I CYCGRG 3 cut(s) 149, 156, 184
BmgT120I GGNCC 2 cut(s) 393, 873
BmiI GGNNCC 2 cut(s) 394, 965
BmrFI CCNGG 2 cut(s) 470, 587
BplI GAGNNNNNCTC 2 cut(s) 1074, 1106
BpmI CTGGAG 1 cut(s) 1076
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 2 cut(s) 954, 1124
BsaAI YACGTR 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 183
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 821, 838
Bse21I CCTNAGG 1 cut(s) 715
Bse3DI GCAATG 3 cut(s) 330, 402, 480
BseBI CCWGG 2 cut(s) 470, 587
BseDI CCNNGG 1 cut(s) 183
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 3 cut(s) 330, 402, 480
BseMII CTCAG 4 cut(s) 59, 264, 648, 729
BseNI ACTGG 2 cut(s) 821, 838
BseXI GCAGC 5 cut(s) 105, 394, 535, 535, 778
BsgI GTGCAG 1 cut(s) 542
BshFI GGCC 1 cut(s) 590
BshNI GGYRCC 1 cut(s) 963
BsiHKAI GWGCWC 1 cut(s) 1173
BsiHKCI CYCGRG 3 cut(s) 149, 156, 184
BslFI GGGAC 2 cut(s) 924, 1072
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 924, 1072
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 1 cut(s) 590
BsoBI CYCGRG 3 cut(s) 149, 156, 184
Bsp1286I GDGCHC 1 cut(s) 1173
Bsp143I GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
BspACI CCGC 3 cut(s) 48, 270, 443
BspANI GGCC 1 cut(s) 590
BspCNI CTCAG 4 cut(s) 60, 265, 647, 728
BspLI GGNNCC 2 cut(s) 394, 965
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 4 cut(s) 577, 608, 756, 821
BspT107I GGYRCC 1 cut(s) 963
BspTI CTTAAG 1 cut(s) 365
BsrDI GCAATG 3 cut(s) 330, 402, 480
BsrI ACTGG 2 cut(s) 821, 838
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
BssNAI GTATAC 1 cut(s) 858
BssNI GRCGYC 1 cut(s) 32
Bst1107I GTATAC 1 cut(s) 858
Bst2UI CCWGG 2 cut(s) 470, 587
Bst4CI ACNGT 7 cut(s) 262, 382, 679, 696, 883, 915, 991
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 1128
BstDEI CTNAG 4 cut(s) 68, 273, 634, 715
BstKTI GATC 8 cut(s) 168, 436, 585, 616, 640, 751, 829, 864
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
BstMWI GCNNNNNNNGC 4 cut(s) 305, 407, 557, 800
BstNI CCWGG 2 cut(s) 470, 587
BstNSI RCATGY 1 cut(s) 1076
BstSCI CCNGG 2 cut(s) 468, 585
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 5 cut(s) 105, 394, 535, 535, 778
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BstZ17I GTATAC 1 cut(s) 858
Bsu36I CCTNAGG 1 cut(s) 715
BsuRI GGCC 1 cut(s) 590
BtsIMutI CAGTG 3 cut(s) 258, 618, 692
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 1128
Cfr13I GGNCC 2 cut(s) 393, 873
CseI GACGC 1 cut(s) 21
Csp6I GTAC 3 cut(s) 85, 797, 1178
CspCI CAANNNNNGTGG 2 cut(s) 629, 664
CviAII CATG 6 cut(s) 314, 439, 556, 664, 886, 1073
CviQI GTAC 3 cut(s) 85, 797, 1178
DdeI CTNAG 4 cut(s) 68, 273, 634, 715
DpnI GATC 8 cut(s) 167, 435, 584, 615, 639, 750, 828, 863
DpnII GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
DrdI GACNNNNNNGTC 1 cut(s) 263
DriI GACNNNNNGTC 1 cut(s) 151
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1105I GACNNNNNGTC 1 cut(s) 151
Eco47I GGWCC 2 cut(s) 393, 873
Eco57I CTGAAG 1 cut(s) 47
Eco72I CACGTG 1 cut(s) 179
Eco81I CCTNAGG 1 cut(s) 715
Eco88I CYCGRG 3 cut(s) 149, 156, 184
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 2 cut(s) 468, 585
FaeI CATG 6 cut(s) 317, 442, 559, 667, 889, 1076
FaqI GGGAC 2 cut(s) 924, 1072
FatI CATG 6 cut(s) 313, 438, 555, 663, 885, 1072
FauI CCCGC 1 cut(s) 436
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 1 cut(s) 857
Fnu4HI GCNGC 6 cut(s) 119, 270, 408, 524, 549, 792
Fsp4HI GCNGC 6 cut(s) 119, 270, 408, 524, 549, 792
FspBI CTAG 2 cut(s) 419, 431
GluI GCNGC 6 cut(s) 119, 270, 408, 524, 549, 792
GsuI CTGGAG 1 cut(s) 1076
HaeIII GGCC 1 cut(s) 590
HgaI GACGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 6 cut(s) 317, 442, 559, 667, 889, 1076
HincII GTYRAC 5 cut(s) 145, 256, 945, 985, 1033
HindII GTYRAC 5 cut(s) 145, 256, 945, 985, 1033
HindIII AAGCTT 2 cut(s) 1109, 1187
HinfI GANTC 6 cut(s) 17, 70, 152, 422, 660, 1085
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 9 cut(s) 53, 100, 145, 256, 462, 858, 945, 985, 1033
Hpy188I TCNGA 6 cut(s) 16, 27, 75, 134, 630, 637
Hpy188III TCNNGA 8 cut(s) 158, 186, 717, 926, 1093, 1103, 1115, 1142
Hpy8I GTNNAC 9 cut(s) 53, 100, 145, 256, 462, 858, 945, 985, 1033
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 3 cut(s) 22, 191, 199
HpyCH4III ACNGT 7 cut(s) 262, 382, 679, 696, 883, 915, 991
HpyCH4IV ACGT 3 cut(s) 55, 178, 483
HpyCH4V TGCA 8 cut(s) 230, 299, 344, 523, 692, 708, 743, 896
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 407, 557, 800
HpyF3I CTNAG 4 cut(s) 68, 273, 634, 715
HpySE526I ACGT 3 cut(s) 55, 178, 483
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 6 cut(s) 317, 442, 559, 667, 889, 1076
Kzo9I GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
Lsp1109I GCAGC 5 cut(s) 105, 394, 535, 535, 778
MaeI CTAG 2 cut(s) 419, 431
MaeII ACGT 3 cut(s) 55, 178, 483
MaeIII GTNAC 1 cut(s) 766
MalI GATC 8 cut(s) 167, 435, 584, 615, 639, 750, 828, 863
MboI GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
MboII GAAGA 3 cut(s) 340, 490, 1061
MfeI CAATTG 1 cut(s) 641
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 1173
MluCI AATT 9 cut(s) 224, 351, 512, 599, 603, 641, 868, 929, 1052
MlyI GAGTC 3 cut(s) 79, 161, 1094
MmeI TCCRAC 2 cut(s) 53, 360
MseI TTAA 5 cut(s) 191, 237, 366, 486, 602
MspA1I CMGCKG 2 cut(s) 272, 611
MspCI CTTAAG 1 cut(s) 365
MspR9I CCNGG 2 cut(s) 470, 587
MunI CAATTG 1 cut(s) 641
Mva1269I GAATGC 1 cut(s) 1157
MvaI CCWGG 2 cut(s) 470, 587
MwoI GCNNNNNNNGC 4 cut(s) 305, 407, 557, 800
NdeI CATATG 1 cut(s) 949
NdeII GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
NlaIII CATG 6 cut(s) 317, 442, 559, 667, 889, 1076
NlaIV GGNNCC 2 cut(s) 394, 965
NspI RCATGY 1 cut(s) 1076
PaqCI CACCTGC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PctI GAATGC 1 cut(s) 1157
PfeI GAWTC 3 cut(s) 17, 422, 660
PflFI GACNNNGTC 2 cut(s) 57, 69
PkrI GCNGC 6 cut(s) 120, 271, 409, 525, 550, 793
PleI GAGTC 3 cut(s) 78, 160, 1093
PmaCI CACGTG 1 cut(s) 179
PmlI CACGTG 1 cut(s) 179
PpsI GAGTC 3 cut(s) 78, 160, 1093
Ppu21I YACGTR 1 cut(s) 179
PshBI ATTAAT 1 cut(s) 602
Psp1406I AACGTT 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 468, 585
PspCI CACGTG 1 cut(s) 179
PspGI CCWGG 2 cut(s) 468, 585
PspN4I GGNNCC 2 cut(s) 394, 965
PspPI GGNCC 2 cut(s) 393, 873
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 2 cut(s) 57, 69
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 3 cut(s) 86, 798, 1179
RsaNI GTAC 3 cut(s) 85, 797, 1178
SaqAI TTAA 5 cut(s) 191, 237, 366, 486, 602
SatI GCNGC 6 cut(s) 119, 270, 408, 524, 549, 792
Sau3AI GATC 8 cut(s) 165, 433, 582, 613, 637, 748, 826, 861
Sau96I GGNCC 2 cut(s) 393, 873
SchI GAGTC 3 cut(s) 79, 161, 1094
ScrFI CCNGG 2 cut(s) 470, 587
SduI GDGCHC 1 cut(s) 1173
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 393, 873
SmlI CTYRAG 3 cut(s) 365, 969, 1103
SmoI CTYRAG 3 cut(s) 365, 969, 1103
Sse9I AATT 9 cut(s) 224, 351, 512, 599, 603, 641, 868, 929, 1052
SsiI CCGC 3 cut(s) 48, 270, 443
SspMI CTAG 2 cut(s) 419, 431
StyD4I CCNGG 2 cut(s) 468, 585
TaaI ACNGT 7 cut(s) 262, 382, 679, 696, 883, 915, 991
TaiI ACGT 3 cut(s) 58, 181, 486
TaqI TCGA 2 cut(s) 113, 168
TasI AATT 9 cut(s) 224, 351, 512, 599, 603, 641, 868, 929, 1052
TatI WGTACW 2 cut(s) 796, 1177
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 3 cut(s) 17, 422, 660
Tru1I TTAA 5 cut(s) 191, 237, 366, 486, 602
Tru9I TTAA 5 cut(s) 191, 237, 366, 486, 602
TscAI CASTG 3 cut(s) 265, 625, 699
TseI GCWGC 5 cut(s) 118, 407, 523, 548, 791
TspDTI ATGAA 6 cut(s) 97, 231, 330, 341, 491, 1127
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 3 cut(s) 265, 625, 699
Tth111I GACNNNGTC 2 cut(s) 57, 69
Vha464I CTTAAG 1 cut(s) 365
VpaK11BI GGWCC 2 cut(s) 393, 873
VspI ATTAAT 1 cut(s) 602
XapI RAATTY 1 cut(s) 929
XceI RCATGY 1 cut(s) 1076
XcmI CCANNNNNNNNNTGG 2 cut(s) 503, 883
XmiI GTMKAC 1 cut(s) 857
XspI CTAG 2 cut(s) 419, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.