Rorug05G0475300

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003838
Physical Location & Seq
Reverse (-)
65289415 .. 65291460
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0475300.1

Sequence Viewer

Length: 1146 bp
ATGCAGCGTAAAAGGAATGGTTCAAACGTGATGAAGGGAGGCCTTTTGGATGTTTCTCCATGCTCCAAGGACCCTAATCAGTTGAAAAACATGGTTCAAAAAGAGACTTTGGAAGAAAATGTGTCACCGAATCATATTCAAGTGCCACAGCATGTGGAGGGGGCAGAATATGTGGATTTGACATTGCTTGACTCTTCAAAAAAGTCATCTGGAGCAGAGATTAAATGCAAGTTGGCATTAGGTTATGTTGAACATATTGTTGCTTATGCTACTGTTATGGACTGTGATGATCACAATCAGCTTATTCATGGTGCTCCACTTGGGGATGATAATCTGCGTGTTTCTATCTATGAGGCGGTTGAGGAGAAAGCAAAGGTCCCATTTCTGATTAAAGTTGAAATCGAAACTGTTAAGCAGGCTATGGGGAGTTGGGTAGCATGGCCTAAAGAGCTAATCATAAGGTCACTTGTGAAGAAACCTATCAAGGATAATGCTGAGGAGAAAAGGAAAAAGAAAGAGACAGCGGATGAAGACTCAGAGTGGGGTGAAGACAAATTCAGAGACGGGCGTACCATCAGCTTCCACTTTGAACCTGAAGTCTTCGGATATTCTCGCAAGACCTTTCTAATGGGATCAGATGTTCGGTATCTCTATGACCAGTTGATTGCTTATAAAATGGTAGACATGGTTGCATTCATTGACCCTTCTCTAATTGGTGCAAGTGGGACTGGGAATGGAACTATTAGGGCCCAACACATTAGAGATAGACTTGTAACTGCAAAACCAGGACAGATGTTCATTGATCATTGGATGTTGACAATTGTTGATCCGGACCAAGGCACTGCCTATTTTTTGGATCCTCTGAAAAGACGCTTACCCACCGGAGATTGGATGTCCATAGTGAAAACGAAGAGGAAAGGCCGGAGTTCAGTTATGTGGAAAAATTTGGCTGGCATTCCTCCCCAACCTAGCAACAAGGAGTGTGGGTACTTTATCATGCGATACATGAGGGATATTATTGAGGATAAAGACCTGTCATTATTTCCTGTAAAGTGGGAGAGGAGAGGTAGCAGCCACTATACCCAAGCAGACATTGATCAGATGCGAAATGAGTGGGCAAAGTTTGTGGTCAAGACATATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

381

Amino Acids

43.58

Weight (kDa)

8.14

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 73 - 153 5.1e-12 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 672
AccI GTMKAC 1 cut(s) 681
AccIII TCCGGA 1 cut(s) 829
AciI CCGC 2 cut(s) 356, 524
AclWI GGATC 4 cut(s) 640, 821, 851, 864
AcsI RAATTY 2 cut(s) 554, 943
AcuI CTGAAG 1 cut(s) 615
AfaI GTAC 2 cut(s) 571, 989
AfiI CCNNNNNNNGG 2 cut(s) 836, 888
AgsI TTSAA 8 cut(s) 24, 85, 98, 140, 198, 251, 398, 590
AjnI CCWGG 1 cut(s) 784
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
AluBI AGCT 3 cut(s) 301, 451, 579
AluI AGCT 3 cut(s) 301, 451, 579
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 3 cut(s) 98, 512, 555
AlwI GGATC 4 cut(s) 640, 821, 851, 864
Aor13HI TCCGGA 1 cut(s) 829
AoxI GGCC 4 cut(s) 40, 440, 747, 919
ApaI GGGCCC 1 cut(s) 751
ApeKI GCWGC 2 cut(s) 4, 1071
ApoI RAATTY 2 cut(s) 554, 943
AspS9I GGNCC 5 cut(s) 70, 376, 747, 748, 832
AsuHPI GGTGA 2 cut(s) 117, 557
AvaII GGWCC 3 cut(s) 70, 376, 832
BaeGI GKGCMC 1 cut(s) 751
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BamHI GGATCC 1 cut(s) 856
BanII GRGCYC 1 cut(s) 751
BbsI GAAGAC 3 cut(s) 537, 555, 592
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 2 cut(s) 16, 1083
BccI CCATC 1 cut(s) 581
BciT130I CCWGG 1 cut(s) 786
BclI TGATCA 3 cut(s) 289, 802, 1096
BcoDI GTCTC 3 cut(s) 98, 512, 555
BfaI CTAG 1 cut(s) 969
BisI GCNGC 2 cut(s) 5, 1072
BlsI GCNGC 2 cut(s) 6, 1073
Bme1390I CCNGG 1 cut(s) 786
Bme18I GGWCC 3 cut(s) 70, 376, 832
BmgT120I GGNCC 5 cut(s) 70, 376, 747, 748, 832
BmiI GGNNCC 4 cut(s) 72, 378, 749, 858
BmrFI CCNGG 1 cut(s) 786
BmrI ACTGGG 1 cut(s) 738
BmsI GCATC 1 cut(s) 1092
BmuI ACTGGG 1 cut(s) 738
BpiI GAAGAC 3 cut(s) 537, 555, 592
BpmI CTGGAG 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 495
BsaBI GATNNNNATC 2 cut(s) 294, 330
BsaJI CCNNGG 2 cut(s) 66, 835
BsaWI WCCGGW 2 cut(s) 829, 881
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 836, 888
Bse1I ACTGG 2 cut(s) 658, 733
Bse3DI GCAATG 1 cut(s) 182
Bse8I GATNNNNATC 2 cut(s) 294, 330
BseAI TCCGGA 1 cut(s) 829
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 66, 835
BseGI GGATG 5 cut(s) 55, 331, 532, 816, 897
BseJI GATNNNNATC 2 cut(s) 294, 330
BseLI CCNNNNNNNGG 2 cut(s) 836, 888
BseMI GCAATG 1 cut(s) 182
BseMII CTCAG 2 cut(s) 486, 549
BseNI ACTGG 2 cut(s) 658, 733
BseRI GAGGAG 3 cut(s) 377, 512, 1075
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 2 cut(s) 16, 1083
BshFI GGCC 4 cut(s) 42, 442, 749, 921
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 3 cut(s) 830, 882, 922
BslFI GGGAC 2 cut(s) 362, 739
BslI CCNNNNNNNGG 2 cut(s) 836, 888
BsmAI GTCTC 3 cut(s) 98, 512, 555
BsmBI CGTCTC 1 cut(s) 555
BsmFI GGGAC 2 cut(s) 362, 739
BsmI GAATGC 2 cut(s) 692, 954
BsnI GGCC 4 cut(s) 42, 442, 749, 921
Bsp120I GGGCCC 1 cut(s) 747
Bsp1286I GDGCHC 2 cut(s) 316, 751
Bsp13I TCCGGA 1 cut(s) 829
Bsp143I GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
BspACI CCGC 2 cut(s) 356, 524
BspANI GGCC 4 cut(s) 42, 442, 749, 921
BspCNI CTCAG 2 cut(s) 487, 548
BspEI TCCGGA 1 cut(s) 829
BspLI GGNNCC 4 cut(s) 72, 378, 749, 858
BspPI GGATC 4 cut(s) 640, 821, 851, 864
BsrDI GCAATG 1 cut(s) 182
BsrI ACTGG 2 cut(s) 658, 733
BssECI CCNNGG 2 cut(s) 66, 835
BssMI GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
BssT1I CCWWGG 2 cut(s) 66, 835
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 3 cut(s) 274, 284, 409
Bst6I CTCTTC 2 cut(s) 199, 905
BstC8I GCNNGC 2 cut(s) 417, 952
BstDEI CTNAG 2 cut(s) 495, 535
BstF5I GGATG 5 cut(s) 55, 331, 532, 816, 897
BstKTI GATC 6 cut(s) 292, 635, 805, 829, 859, 1099
BstMAI GTCTC 3 cut(s) 98, 512, 555
BstMBI GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
BstMWI GCNNNNNNNGC 1 cut(s) 448
BstNI CCWGG 1 cut(s) 786
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 784
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 2 cut(s) 16, 1083
BstV2I GAAGAC 3 cut(s) 537, 555, 592
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
BsuRI GGCC 4 cut(s) 42, 442, 749, 921
BtsCI GGATG 5 cut(s) 55, 331, 532, 816, 897
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 1 cut(s) 840
Cac8I GCNNGC 2 cut(s) 417, 952
Cfr13I GGNCC 5 cut(s) 70, 376, 747, 748, 832
CseI GACGC 1 cut(s) 879
Csp6I GTAC 2 cut(s) 570, 988
CspCI CAANNNNNGTGG 4 cut(s) 964, 999, 1107, 1142
CviAII CATG 8 cut(s) 60, 91, 152, 308, 438, 685, 997, 1006
CviQI GTAC 2 cut(s) 570, 988
DdeI CTNAG 2 cut(s) 495, 535
DpnI GATC 6 cut(s) 291, 634, 804, 828, 858, 1098
DpnII GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
Eam1104I CTCTTC 2 cut(s) 199, 905
EarI CTCTTC 2 cut(s) 199, 905
Eco130I CCWWGG 2 cut(s) 66, 835
Eco147I AGGCCT 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 751
Eco47I GGWCC 3 cut(s) 70, 376, 832
Eco57I CTGAAG 1 cut(s) 615
EcoO109I RGGNCCY 3 cut(s) 70, 376, 747
EcoRII CCWGG 1 cut(s) 784
EcoT14I CCWWGG 2 cut(s) 66, 835
EcoT38I GRGCYC 1 cut(s) 751
ErhI CCWWGG 2 cut(s) 66, 835
Esp3I CGTCTC 1 cut(s) 555
FaeI CATG 8 cut(s) 63, 94, 155, 311, 441, 688, 1000, 1009
FaqI GGGAC 2 cut(s) 362, 739
FatI CATG 8 cut(s) 59, 90, 151, 307, 437, 684, 996, 1005
FauNDI CATATG 1 cut(s) 1138
FbaI TGATCA 3 cut(s) 289, 802, 1096
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 2 cut(s) 5, 1072
FokI GGATG 5 cut(s) 62, 338, 539, 823, 904
FriOI GRGCYC 1 cut(s) 751
Fsp4HI GCNGC 2 cut(s) 5, 1072
FspBI CTAG 1 cut(s) 969
GluI GCNGC 2 cut(s) 5, 1072
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 4 cut(s) 42, 442, 749, 921
HapII CCGG 3 cut(s) 830, 882, 922
HgaI GACGC 1 cut(s) 879
Hin1II CATG 8 cut(s) 63, 94, 155, 311, 441, 688, 1000, 1009
HincII GTYRAC 1 cut(s) 816
HindII GTYRAC 1 cut(s) 816
HinfI GANTC 3 cut(s) 130, 191, 533
HpaII CCGG 3 cut(s) 830, 882, 922
HphI GGTGA 2 cut(s) 117, 557
Hpy166II GTNNAC 2 cut(s) 682, 816
Hpy188I TCNGA 7 cut(s) 387, 538, 560, 605, 637, 864, 1101
Hpy188III TCNNGA 3 cut(s) 210, 830, 1132
Hpy8I GTNNAC 2 cut(s) 682, 816
HpyAV CCTTC 2 cut(s) 28, 714
HpyCH4III ACNGT 3 cut(s) 274, 284, 409
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 5 cut(s) 4, 228, 692, 719, 779
HpyF10VI GCNNNNNNNGC 1 cut(s) 448
HpyF3I CTNAG 2 cut(s) 495, 535
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 8 cut(s) 63, 94, 155, 311, 441, 688, 1000, 1009
Kpn2I TCCGGA 1 cut(s) 829
Ksp22I TGATCA 3 cut(s) 289, 802, 1096
Kzo9I GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
LmnI GCTCC 3 cut(s) 68, 212, 319
Lsp1109I GCAGC 2 cut(s) 16, 1083
LweI GCATC 1 cut(s) 1092
MaeI CTAG 1 cut(s) 969
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 3 cut(s) 123, 462, 772
MalI GATC 6 cut(s) 291, 634, 804, 828, 858, 1098
MboI GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
MboII GAAGA 7 cut(s) 125, 186, 484, 542, 560, 592, 922
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 2 cut(s) 316, 751
MluCI AATT 4 cut(s) 554, 711, 819, 943
MlyI GAGTC 2 cut(s) 185, 527
MroI TCCGGA 1 cut(s) 829
MseI TTAA 3 cut(s) 222, 390, 411
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 3 cut(s) 830, 882, 922
MspR9I CCNGG 1 cut(s) 786
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 2 cut(s) 692, 954
MvaI CCWGG 1 cut(s) 786
MwoI GCNNNNNNNGC 1 cut(s) 448
NdeI CATATG 1 cut(s) 1138
NdeII GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
NlaIII CATG 8 cut(s) 63, 94, 155, 311, 441, 688, 1000, 1009
NlaIV GGNNCC 4 cut(s) 72, 378, 749, 858
NmuCI GTSAC 2 cut(s) 123, 462
NspI RCATGY 1 cut(s) 155
PceI AGGCCT 1 cut(s) 42
PctI GAATGC 2 cut(s) 692, 954
PfeI GAWTC 1 cut(s) 130
PkrI GCNGC 2 cut(s) 6, 1073
PleI GAGTC 2 cut(s) 185, 527
PpsI GAGTC 2 cut(s) 185, 527
PpuMI RGGWCCY 2 cut(s) 70, 376
PsiI TTATAA 1 cut(s) 672
Psp5II RGGWCCY 2 cut(s) 70, 376
Psp6I CCWGG 1 cut(s) 784
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 4 cut(s) 72, 378, 749, 858
PspOMI GGGCCC 1 cut(s) 747
PspPI GGNCC 5 cut(s) 70, 376, 747, 748, 832
PspPPI RGGWCCY 2 cut(s) 70, 376
PsuI RGATCY 1 cut(s) 856
RsaI GTAC 2 cut(s) 571, 989
RsaNI GTAC 2 cut(s) 570, 988
SaqAI TTAA 3 cut(s) 222, 390, 411
SatI GCNGC 2 cut(s) 5, 1072
Sau3AI GATC 6 cut(s) 289, 632, 802, 826, 856, 1096
Sau96I GGNCC 5 cut(s) 70, 376, 747, 748, 832
SchI GAGTC 2 cut(s) 185, 527
ScrFI CCNGG 1 cut(s) 786
SduI GDGCHC 2 cut(s) 316, 751
SfaNI GCATC 1 cut(s) 1092
SinI GGWCC 3 cut(s) 70, 376, 832
Sse9I AATT 4 cut(s) 554, 711, 819, 943
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 2 cut(s) 356, 524
SspMI CTAG 1 cut(s) 969
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 784
StyI CCWWGG 2 cut(s) 66, 835
TaaI ACNGT 3 cut(s) 274, 284, 409
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 402
TasI AATT 4 cut(s) 554, 711, 819, 943
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 3 cut(s) 222, 390, 411
Tru9I TTAA 3 cut(s) 222, 390, 411
TscAI CASTG 1 cut(s) 847
TseFI GTSAC 2 cut(s) 123, 462
TseI GCWGC 2 cut(s) 4, 1071
Tsp45I GTSAC 2 cut(s) 123, 462
TspDTI ATGAA 5 cut(s) 47, 296, 543, 685, 787
TspRI CASTG 1 cut(s) 847
VpaK11BI GGWCC 3 cut(s) 70, 376, 832
XapI RAATTY 2 cut(s) 554, 943
XceI RCATGY 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 681
XspI CTAG 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.