Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003838

Physical Location & Seq

Genomic Coordinates Reverse (-)

898325 .. 902208

Gene Structure 3884 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1011700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1224 bp

ATGGAGGAAAAATCGGAAACGAGCGGTGTAAAAACGCCGCTCTTCACACCGCTGTTGGGGAGCGGTGGACGCAGTCGCCGACTGAGTCGAAGAAACTCGTACAACTCACTCAGAAAGGACTTCATTTCGAGGCTCCCTGACAAGGTCCGTGCCGGTGTTGACCCCGAGTCTCCTGACGGCATTGACTTCTCTCGAACCTCCGCCTTGAACCGTGGAGAGAAGGAGTACTATGAAGCCCAATTTGAAACACTTAAATCCTTTGAGGAGGTTGACGCTGTGGCGGCAGCCGACAGCATCGATGATGAGGATCTCGAAGAACAAGCACAACATGAAAGAGCAATGAAGATTTCCAACTATGCCAATATTATTCTCTTGGGACTTAAGATCTATGCTACTGTAAAGAGTGGATCGATAGCCATTGCTGCATCGACTCTAGATTCTTTGCTCGATCTCATGGCTGGTGGAATACTTTGGTTCACTCACCTTGCAATGAAGAGCGTCAATATCTATAAATATCCTATCGGAAAATTGAGGGTGCAGCCTGTTGGCATAATTGTCTTTGCTGCTGTCATGGCTACCCTCGGGTTTCAGGTATTCATCCAGGCTTTAGAACAACTAATAACCGGTAATGCCACTGAAAAGATGTCCGCAGAGCAATTGGCGTGGTTGTACGCTATCATGATATTTGCCACCGTGGTAAAGCTTGCACTCTGGTTATACTGCAGGACCTCAGGGAACAAGATTGTCCGTGCCTACGCCAAGGATCACTATTTTGATGTGGTAACAAACGTGGTAGGATTAGTGGCTGCTGTTCTTGCTGATAAGTTTTTGTGGTGGATTGATCCTGCTGGTGCTATTATCCTTGCTGTATACACGATTACAAATTGGTCCGGAACTGTTCTAGAAAATGCAGTTTCCCTCGTGGGACAATCAGCGCCTCCGGAATTCCTACAAAAACTGACATACCTTACCATAAGGCACCCTCAAGTGAAGCGTATTGACACAGTTCGTGCTTACACATTTGGTGTTCTTTATTTTGTAGAGGTTGACATTGAACTCCCGGAGGAGTTGCCCTTGAAAGAGGCACATATCATTGGAGAGACCCTCCAGATAAAGCTTGAGAAACTTCCTGAAGTTGAGCGAGCATTTGTTCACCTTGACTTTGAATGTGACCACAAACCGGAGCATTCTGTACTCGTCAAGCTTCCGAACAGTCAGCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux ZT_dimer

Protein Analysis

408

Amino Acids

45.43

Weight (kDa)

5.62

Isoelectric Point (pI)

43.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	115 - 308	3.7e-26	Cation efflux transmembrane domain
ZT_dimer	PF16916	313 - 387	4.8e-07	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003838)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58060 AT3G58060
fragaria_vesca	FvH4_3g44550 FvH4_3g44550 FvH4_3g44550
malus_domestica	MD03G1011700.v1.1 MD11G1014600.v1.1
prunus_persica	Prupe.6G012100_v2.0.a1 Prupe.7G081200_v2.0.a1
pyrus_communis	pycom11g00990
rosa_chinensis	RchiOBHm_Chr3g0479221 RchiOBHm_Chr5g0081261
rosa_laevigata	RLG00000023593 RLG00000036922
rosa_multiflora	Rmu_sc0007768.1_g000006 Rmu_ssc0000432.1_g000058
rosa_roxburghii	Rroxscaffold_1G00002230 Rroxscaffold_6G00402530
rosa_rugosa	Rorug03G0172300 Rorug03G0172800 Rorug03G0172800 Rorug05G0475200 Rorug05G0475300
rosa_samantha	Rh3AG223900 Rh3BG256800 Rh3CG252500 Rh3DG248700 Rh5AG526900 Rh5BG551100 Rh5CG574000 Rh5DG561100
rosa_wichuraiana	Rw3G020070 Rw3G020530 Rw5G048980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	978
AccBSI	CCGCTC	3 cut(s)	24, 40, 63
AccI	GTMKAC	1 cut(s)	870
AccIII	TCCGGA	2 cut(s)	890, 940
AciI	CCGC	7 cut(s)	24, 38, 50, 63, 201, 281, 648
AclWI	GGATC	4 cut(s)	315, 415, 771, 836
AcsI	RAATTY	1 cut(s)	944
AcuI	CTGAAG	1 cut(s)	1152
AfaI	GTAC	4 cut(s)	101, 227, 671, 1194
AfiI	CCNNNNNNNGG	3 cut(s)	56, 142, 1180
AflII	CTTAAG	1 cut(s)	380
AgeI	ACCGGT	1 cut(s)	623
AgsI	TTSAA	5 cut(s)	208, 245, 1055, 1078, 1166
AhdI	GACNNNNNGTC	1 cut(s)	166
AjnI	CCWGG	1 cut(s)	600
AluBI	AGCT	3 cut(s)	703, 1117, 1204
AluI	AGCT	3 cut(s)	703, 1117, 1204
Alw26I	GTCTC	2 cut(s)	174, 1094
AlwI	GGATC	4 cut(s)	315, 415, 771, 836
Ama87I	CYCGRG	2 cut(s)	164, 581
Aor13HI	TCCGGA	2 cut(s)	890, 940
ApeKI	GCWGC	5 cut(s)	284, 422, 538, 563, 806
ApoI	RAATTY	1 cut(s)	944
AsiGI	ACCGGT	1 cut(s)	623
AspLEI	GCGC	1 cut(s)	937
AspS9I	GGNCC	3 cut(s)	145, 726, 888
AsuC2I	CCSGG	1 cut(s)	1061
AsuHPI	GGTGA	2 cut(s)	473, 1145
AvaI	CYCGRG	2 cut(s)	164, 581
AvaII	GGWCC	3 cut(s)	145, 726, 888
AxyI	CCTNAGG	1 cut(s)	730
BanI	GGYRCC	1 cut(s)	978
BauI	CACGAG	1 cut(s)	920
BbvI	GCAGC	5 cut(s)	296, 409, 550, 550, 793
BceAI	ACGGC	1 cut(s)	193
BciT130I	CCWGG	1 cut(s)	602
BcnI	CCSGG	1 cut(s)	1061
BcoDI	GTCTC	2 cut(s)	174, 1094
BfaI	CTAG	2 cut(s)	434, 902
BfmI	CTRYAG	1 cut(s)	721
BfoI	RGCGCY	1 cut(s)	938
BfrI	CTTAAG	1 cut(s)	380
BglII	AGATCT	1 cut(s)	384
BisI	GCNGC	7 cut(s)	38, 282, 285, 423, 539, 564, 807
BlsI	GCNGC	7 cut(s)	39, 283, 286, 424, 540, 565, 808
BmcAI	AGTACT	1 cut(s)	227
Bme1390I	CCNGG	2 cut(s)	602, 1061
Bme18I	GGWCC	3 cut(s)	145, 726, 888
BmeRI	GACNNNNNGTC	1 cut(s)	166
BmeT110I	CYCGRG	2 cut(s)	164, 581
BmgT120I	GGNCC	3 cut(s)	145, 726, 888
BmiI	GGNNCC	2 cut(s)	134, 980
BmrFI	CCNGG	2 cut(s)	602, 1061
BmsI	GCATC	2 cut(s)	303, 434
BplI	GAGNNNNNCTC	2 cut(s)	1089, 1121
BpmI	CTGGAG	1 cut(s)	1091
BpuEI	CTTGAG	2 cut(s)	969, 1139
BpuMI	CCSGG	1 cut(s)	1061
Bsa29I	ATCGAT	2 cut(s)	297, 410
BsaBI	GATNNNNATC	1 cut(s)	306
BsaI	GGTCTC	1 cut(s)	1094
BsaJI	CCNNGG	4 cut(s)	211, 580, 693, 759
BsaWI	WCCGGW	4 cut(s)	623, 890, 940, 1180
Bsc4I	CCNNNNNNNGG	3 cut(s)	56, 142, 1180
Bse118I	RCCGGY	2 cut(s)	152, 623
Bse21I	CCTNAGG	1 cut(s)	730
Bse3DI	GCAATG	3 cut(s)	345, 417, 495
Bse8I	GATNNNNATC	1 cut(s)	306
BseAI	TCCGGA	2 cut(s)	890, 940
BseBI	CCWGG	1 cut(s)	602
BseCI	ATCGAT	2 cut(s)	297, 410
BseDI	CCNNGG	4 cut(s)	211, 580, 693, 759
BseGI	GGATG	1 cut(s)	597
BseJI	GATNNNNATC	1 cut(s)	306
BseLI	CCNNNNNNNGG	3 cut(s)	56, 142, 1180
BseMI	GCAATG	3 cut(s)	345, 417, 495
BseMII	CTCAG	3 cut(s)	74, 124, 744
BseRI	GAGGAG	2 cut(s)	278, 1079
BseXI	GCAGC	5 cut(s)	296, 409, 550, 550, 793
BsgI	GTGCAG	1 cut(s)	557
BshNI	GGYRCC	1 cut(s)	978
BshTI	ACCGGT	1 cut(s)	623
BshVI	ATCGAT	2 cut(s)	297, 410
BsiHKCI	CYCGRG	2 cut(s)	164, 581
BsiSI	CCGG	6 cut(s)	153, 624, 891, 941, 1061, 1181
BslFI	GGGAC	2 cut(s)	390, 939
BslI	CCNNNNNNNGG	3 cut(s)	56, 142, 1180
BsmAI	GTCTC	2 cut(s)	174, 1094
BsmFI	GGGAC	2 cut(s)	390, 939
BsmI	GAATGC	1 cut(s)	1186
Bso31I	GGTCTC	1 cut(s)	1094
BsoBI	CYCGRG	2 cut(s)	164, 581
Bsp13I	TCCGGA	2 cut(s)	890, 940
Bsp143I	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
BspACI	CCGC	7 cut(s)	24, 38, 50, 63, 201, 281, 648
BspCNI	CTCAG	3 cut(s)	75, 123, 743
BspDI	ATCGAT	2 cut(s)	297, 410
BspEI	TCCGGA	2 cut(s)	890, 940
BspHI	TCATGA	1 cut(s)	678
BspLI	GGNNCC	2 cut(s)	134, 980
BspMAI	CTGCAG	1 cut(s)	725
BspPI	GGATC	4 cut(s)	315, 415, 771, 836
BspQI	GCTCTTC	2 cut(s)	47, 488
BspT107I	GGYRCC	1 cut(s)	978
BspTI	CTTAAG	1 cut(s)	380
BspTNI	GGTCTC	1 cut(s)	1094
BsrBI	CCGCTC	3 cut(s)	24, 40, 63
BsrDI	GCAATG	3 cut(s)	345, 417, 495
BsrFI	RCCGGY	2 cut(s)	152, 623
BssAI	RCCGGY	2 cut(s)	152, 623
BssECI	CCNNGG	4 cut(s)	211, 580, 693, 759
BssMI	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
BssNAI	GTATAC	1 cut(s)	871
BssSI	CACGAG	1 cut(s)	920
BssT1I	CCWWGG	1 cut(s)	759
Bst1107I	GTATAC	1 cut(s)	871
Bst2BI	CACGAG	1 cut(s)	920
Bst2UI	CCWGG	1 cut(s)	602
Bst4CI	ACNGT	6 cut(s)	212, 397, 694, 898, 1006, 1214
Bst6I	CTCTTC	2 cut(s)	47, 488
BstAFI	CTTAAG	1 cut(s)	380
BstC8I	GCNNGC	2 cut(s)	705, 1143
BstDEI	CTNAG	3 cut(s)	83, 110, 730
BstDSI	CCRYGG	2 cut(s)	211, 693
BstF5I	GGATG	1 cut(s)	597
BstH2I	RGCGCY	1 cut(s)	938
BstHHI	GCGC	1 cut(s)	937
BstKTI	GATC	6 cut(s)	310, 387, 410, 451, 766, 844
BstMAI	GTCTC	2 cut(s)	174, 1094
BstMBI	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
BstMWI	GCNNNNNNNGC	5 cut(s)	69, 281, 422, 572, 815
BstNI	CCWGG	1 cut(s)	602
BstSCI	CCNGG	2 cut(s)	600, 1059
BstSFI	CTRYAG	1 cut(s)	721
BstV1I	GCAGC	5 cut(s)	296, 409, 550, 550, 793
BstX2I	RGATCY	2 cut(s)	307, 384
BstYI	RGATCY	2 cut(s)	307, 384
BstZ17I	GTATAC	1 cut(s)	871
Bsu15I	ATCGAT	2 cut(s)	297, 410
Bsu36I	CCTNAGG	1 cut(s)	730
BsuTUI	ATCGAT	2 cut(s)	297, 410
BtgI	CCRYGG	2 cut(s)	211, 693
BtsCI	GGATG	1 cut(s)	597
BtsIMutI	CAGTG	1 cut(s)	633
Cac8I	GCNNGC	2 cut(s)	705, 1143
CciI	TCATGA	1 cut(s)	678
CfoI	GCGC	1 cut(s)	937
Cfr10I	RCCGGY	2 cut(s)	152, 623
Cfr13I	GGNCC	3 cut(s)	145, 726, 888
ClaI	ATCGAT	2 cut(s)	297, 410
CseI	GACGC	3 cut(s)	78, 281, 487
Csp6I	GTAC	4 cut(s)	100, 226, 670, 1193
CspAI	ACCGGT	1 cut(s)	623
CspCI	CAANNNNNGTGG	2 cut(s)	644, 679
CviAII	CATG	4 cut(s)	329, 454, 571, 679
CviQI	GTAC	4 cut(s)	100, 226, 670, 1193
DdeI	CTNAG	3 cut(s)	83, 110, 730
DpnI	GATC	6 cut(s)	309, 386, 409, 450, 765, 843
DpnII	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
DriI	GACNNNNNGTC	1 cut(s)	166
Eam1104I	CTCTTC	2 cut(s)	47, 488
Eam1105I	GACNNNNNGTC	1 cut(s)	166
EarI	CTCTTC	2 cut(s)	47, 488
EciI	GGCGGA	1 cut(s)	190
Eco130I	CCWWGG	1 cut(s)	759
Eco31I	GGTCTC	1 cut(s)	1094
Eco47I	GGWCC	3 cut(s)	145, 726, 888
Eco57I	CTGAAG	1 cut(s)	1152
Eco81I	CCTNAGG	1 cut(s)	730
Eco88I	CYCGRG	2 cut(s)	164, 581
EcoO109I	RGGNCCY	1 cut(s)	726
EcoRI	GAATTC	1 cut(s)	944
EcoRII	CCWGG	1 cut(s)	600
EcoT14I	CCWWGG	1 cut(s)	759
ErhI	CCWWGG	1 cut(s)	759
FaeI	CATG	4 cut(s)	332, 457, 574, 682
FaqI	GGGAC	2 cut(s)	390, 939
FatI	CATG	4 cut(s)	328, 453, 570, 678
FblI	GTMKAC	1 cut(s)	870
Fnu4HI	GCNGC	7 cut(s)	38, 282, 285, 423, 539, 564, 807
FokI	GGATG	1 cut(s)	584
Fsp4HI	GCNGC	7 cut(s)	38, 282, 285, 423, 539, 564, 807
FspBI	CTAG	2 cut(s)	434, 902
GlaI	GCGC	1 cut(s)	936
GluI	GCNGC	7 cut(s)	38, 282, 285, 423, 539, 564, 807
GsuI	CTGGAG	1 cut(s)	1091
HaeII	RGCGCY	1 cut(s)	938
HapII	CCGG	6 cut(s)	153, 624, 891, 941, 1061, 1181
HgaI	GACGC	3 cut(s)	78, 281, 487
HhaI	GCGC	1 cut(s)	937
Hin1II	CATG	4 cut(s)	332, 457, 574, 682
Hin6I	GCGC	1 cut(s)	935
HinP1I	GCGC	1 cut(s)	935
HincII	GTYRAC	3 cut(s)	160, 271, 1048
HindII	GTYRAC	3 cut(s)	160, 271, 1048
HindIII	AAGCTT	3 cut(s)	701, 1115, 1202
HinfI	GANTC	4 cut(s)	85, 167, 430, 437
HpaII	CCGG	6 cut(s)	153, 624, 891, 941, 1061, 1181
HphI	GGTGA	2 cut(s)	473, 1145
Hpy166II	GTNNAC	7 cut(s)	68, 160, 271, 477, 871, 1048, 1153
Hpy188I	TCNGA	4 cut(s)	16, 113, 524, 1209
Hpy8I	GTNNAC	7 cut(s)	68, 160, 271, 477, 871, 1048, 1153
HpyAV	CCTTC	1 cut(s)	214
HpyCH4III	ACNGT	6 cut(s)	212, 397, 694, 898, 1006, 1214
HpyCH4IV	ACGT	1 cut(s)	789
HpyCH4V	TGCA	6 cut(s)	425, 488, 538, 707, 723, 911
HpyF10VI	GCNNNNNNNGC	5 cut(s)	69, 281, 422, 572, 815
HpyF3I	CTNAG	3 cut(s)	83, 110, 730
HpySE526I	ACGT	1 cut(s)	789
Hsp92II	CATG	4 cut(s)	332, 457, 574, 682
HspAI	GCGC	1 cut(s)	935
Kpn2I	TCCGGA	2 cut(s)	890, 940
Kzo9I	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
LguI	GCTCTTC	2 cut(s)	47, 488
LmnI	GCTCC	3 cut(s)	60, 138, 1183
Lsp1109I	GCAGC	5 cut(s)	296, 409, 550, 550, 793
LweI	GCATC	2 cut(s)	303, 434
MaeI	CTAG	2 cut(s)	434, 902
MaeII	ACGT	1 cut(s)	789
MaeIII	GTNAC	2 cut(s)	781, 1169
MalI	GATC	6 cut(s)	309, 386, 409, 450, 765, 843
MbiI	CCGCTC	3 cut(s)	24, 40, 63
MboI	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
MboII	GAAGA	5 cut(s)	34, 102, 326, 355, 505
MfeI	CAATTG	1 cut(s)	656
MflI	RGATCY	2 cut(s)	307, 384
MluCI	AATT	6 cut(s)	239, 527, 552, 656, 883, 944
MlyI	GAGTC	3 cut(s)	94, 176, 424
MmeI	TCCRAC	1 cut(s)	375
MroI	TCCGGA	2 cut(s)	890, 940
MseI	TTAA	2 cut(s)	252, 381
MspA1I	CMGCKG	1 cut(s)	52
MspCI	CTTAAG	1 cut(s)	380
MspI	CCGG	6 cut(s)	153, 624, 891, 941, 1061, 1181
MspR9I	CCNGG	2 cut(s)	602, 1061
MunI	CAATTG	1 cut(s)	656
Mva1269I	GAATGC	1 cut(s)	1186
MvaI	CCWGG	1 cut(s)	602
MwoI	GCNNNNNNNGC	5 cut(s)	69, 281, 422, 572, 815
NciI	CCSGG	1 cut(s)	1061
NdeII	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
NlaIII	CATG	4 cut(s)	332, 457, 574, 682
NlaIV	GGNNCC	2 cut(s)	134, 980
NmuCI	GTSAC	1 cut(s)	1169
PagI	TCATGA	1 cut(s)	678
PciSI	GCTCTTC	2 cut(s)	47, 488
PcsI	WCGNNNNNNNCGW	1 cut(s)	76
PctI	GAATGC	1 cut(s)	1186
PfeI	GAWTC	1 cut(s)	437
PflFI	GACNNNGTC	3 cut(s)	72, 84, 143
PfoI	TCCNGGA	1 cut(s)	1059
PinAI	ACCGGT	1 cut(s)	623
PkrI	GCNGC	7 cut(s)	39, 283, 286, 424, 540, 565, 808
PleI	GAGTC	3 cut(s)	93, 175, 424
PpsI	GAGTC	3 cut(s)	93, 175, 424
PpuMI	RGGWCCY	1 cut(s)	726
Psp5II	RGGWCCY	1 cut(s)	726
Psp6I	CCWGG	1 cut(s)	600
PspGI	CCWGG	1 cut(s)	600
PspN4I	GGNNCC	2 cut(s)	134, 980
PspPI	GGNCC	3 cut(s)	145, 726, 888
PspPPI	RGGWCCY	1 cut(s)	726
PstI	CTGCAG	1 cut(s)	725
PsuI	RGATCY	2 cut(s)	307, 384
PsyI	GACNNNGTC	3 cut(s)	72, 84, 143
RsaI	GTAC	4 cut(s)	101, 227, 671, 1194
RsaNI	GTAC	4 cut(s)	100, 226, 670, 1193
SapI	GCTCTTC	2 cut(s)	47, 488
SaqAI	TTAA	2 cut(s)	252, 381
SatI	GCNGC	7 cut(s)	38, 282, 285, 423, 539, 564, 807
Sau3AI	GATC	6 cut(s)	307, 384, 407, 448, 763, 841
Sau96I	GGNCC	3 cut(s)	145, 726, 888
ScaI	AGTACT	1 cut(s)	227
SchI	GAGTC	3 cut(s)	94, 176, 424
ScrFI	CCNGG	2 cut(s)	602, 1061
SfaNI	GCATC	2 cut(s)	303, 434
SfcI	CTRYAG	1 cut(s)	721
SinI	GGWCC	3 cut(s)	145, 726, 888
SmlI	CTYRAG	3 cut(s)	380, 984, 1118
SmoI	CTYRAG	3 cut(s)	380, 984, 1118
Sse9I	AATT	6 cut(s)	239, 527, 552, 656, 883, 944
SsiI	CCGC	7 cut(s)	24, 38, 50, 63, 201, 281, 648
SspI	AATATT	1 cut(s)	364
SspMI	CTAG	2 cut(s)	434, 902
StyD4I	CCNGG	2 cut(s)	600, 1059
StyI	CCWWGG	1 cut(s)	759
TaaI	ACNGT	6 cut(s)	212, 397, 694, 898, 1006, 1214
TaiI	ACGT	1 cut(s)	792
TaqI	TCGA	8 cut(s)	88, 128, 193, 297, 312, 410, 428, 447
TasI	AATT	6 cut(s)	239, 527, 552, 656, 883, 944
TatI	WGTACW	2 cut(s)	225, 1192
TauI	GCSGC	2 cut(s)	40, 284
TfiI	GAWTC	1 cut(s)	437
Tru1I	TTAA	2 cut(s)	252, 381
Tru9I	TTAA	2 cut(s)	252, 381
TscAI	CASTG	1 cut(s)	640
TseFI	GTSAC	1 cut(s)	1169
TseI	GCWGC	5 cut(s)	284, 422, 538, 563, 806
Tsp45I	GTSAC	1 cut(s)	1169
TspDTI	ATGAA	6 cut(s)	112, 246, 345, 356, 506, 586
TspGWI	ACGGA	2 cut(s)	137, 737
TspRI	CASTG	1 cut(s)	640
Tth111I	GACNNNGTC	3 cut(s)	72, 84, 143
Vha464I	CTTAAG	1 cut(s)	380
VpaK11BI	GGWCC	3 cut(s)	145, 726, 888
XapI	RAATTY	1 cut(s)	944
XbaI	TCTAGA	2 cut(s)	433, 901
XmiI	GTMKAC	1 cut(s)	870
XspI	CTAG	2 cut(s)	434, 902
ZrmI	AGTACT	1 cut(s)	227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.