Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0006344

Physical Location & Seq

Genomic Coordinates Reverse (-)

1803034 .. 1805556

Gene Structure 2523 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g02020.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGCTTGCCAAGCTCCAGCTGGACACGACTTGCTTCCCCATTCTGGGCTCCACAAGAACCTCACCCAGCACAGGACTCCCTCCTCTGGAGAAACTTGAACTCATAACCAGAGATAGGAAGAAGCACAGAGTGCTCAACGGAGGAGGGTTCAAGAAGGGTTTTGGTGAAACTCGCAGACCCAACAATGGTAATGGTGGGAGTGTTGTTCATGGAGTTGGGTTGAAAAAACGGGGTTTTGGGAAGAACCCAGATGAGGAAAAGGGGTTAGAAGATGTGGGTAATGGTGGGTTTGTGGAAAGGAAGGAACTTGGGGTTGAGTTGGGTGGGAATGGTGTGGTGAAGAAAATGCAGACCACGTGTTCGACGAAATGGCTGAGTTACGGTGGGTGCATTCCGGCGATGTTGACGGCGGTGGAGGAGGTTGAGGACTTGGATGAGGCACTGAAGCCGTGGGAGGAGAGGCTGAGTAACAAAGAAAGGAGCATAATTTTGAAGGAGCAGAGGAGTTGGGTGAGAGCTTTGGAGATTTTCGAGTGGTTTAAGAGGAAAGGGTGTTATGAGGTTCATGTGATTCATTACAATATAGTGCTTAGGATTCTTGGGAAGGCGAAGAAGTGGAGACATTTGAGGAGTGTTTGGGATGAGATGAATGTGGAGAGAATAGAACCTATCAACTCGACGTATGGGACTTTGATTGATGTTTATAGTAAAGGTGGGTTTGAGAAAGAAGCGCTTGTTTGGCTGCAGAGGATGACCAAGCAAGGTATGAAGCCTGATGAGGTTACTATGGCGATTGTGCTGCAGTTGTATAAGAAAGCAGGGGAGTATCGGAAAGCTGAGGAGTTTTTCGAGAAATGGTCAGAGAGTGCATTGCATTCTCATGGCTCTTTGAGCTCACATACGTATAACACATTGATCGACACGCATGGAAAGGCTGGCCGGCTTAAAGAAGCATCAGAGATATTTGCACTGATGCTAAGGGAAGGGATTGCTCCAACTACGGTAACTTTCAATACTATGATTCACATTTGTGGTAATCATGGTCAGCTTTCGGAAGTTGATTCACTTATGCAAAAGATGGAAGAGGTTCGATGTCCACCAGACACGAGAACGTATAATATTCTGATTTCCCTCCATGCTAGGCATGATAATATAGACATGGCTACAAACTACTTTGCAAAGATGAAGGAGGCTTGCCTTGAGCCAGACCCTGTGAGTTACCGCACCCTTTTGTATGCATACTCTGTGAGGCACATGGTGAGCAAAGCTGAAGAGCTCGTATCAGAGATGGATGAAAGGGGACTTGAGATTGATGAGTTCACTCATTCGGCTCTGACTAGGATGTACATAGAAGCTGGGATGCTTGAGAAGTCTTGGGTTTGGTTCATGAGATTTCATCTCTCAGGGAAAATGGGATCTGATTGCTATGCTGCCAATATTGATGCATACGGAGAGCGTGGGCATATTTCAGAAGCTGAGAAAGTTTTTAATTGCTGCCGAGAAGTAAACAAGTTGAGTGTCGTTGAGTTCAACGTGATGATCAAAGCGTATGGGGTTGGGAAACAGTACAGTAGAGCTTGCCAGTTGTTTGATAGCATGGAAAGTCATGGTGTAATTCCGGACAGATGTAGCTATAGTTCTCTAATACAAATTTTGGCTAGTGGTGATATGCCACATACCGCAAGGCCTTATCTGAAGAAGATGCATGAGTCAGGGTTGGTACATGATTGCATCCCTTATTGTGCTGTGATATCAAGCTTTGCAAAATTAGGTCAATTGGAAAAGGCGGAGGAAGTATATAAACAGATGGTTGGATTCAGTGTGCAGCCTGATGTTATTGTCTTTGGGGTGTTGATAAATGCTTTTGCTGAAGTTGGATGTGTTAAAGAAGCTCTCAGTTATGCTGATGCAATGAAAAGGGCAGGTTTCCCTGGGAACACAGTTATTTACAATACCTTAATCAAGCTCTATACTAAAGTTGGCCTTTTGAAAGAAGCAGAAGAAACATACAAACTTCTTCTGGCCTCAGAGGATGGTCCTGCTATATATGCCTCAAATTGTATGATTGATCTTTATAGCGAGCGATGTATGGTTAAACCAGCAGAAGAGCTCTTTGACAGCTTGAAGAGCAAGGGGGATGCAAATGAGTTTACATTTGCAATGATGGTGTGTATGTATAAGAGAATGGGGAGGTTTGAGGAGGCCATTCAGATTGCAAAGCAGATGAGGGAACTGCGACTTTTAAGTGATGTGCTGAGTTATAATAATGTTATTGGGTTGTATGCTACATATGGAAGATTCAAAGAAGTGGTGGGAACCTTCAAGGAAATGACGAAGGCAGGCATTCAACCTGATGAATGCACATTCAAATCACTGGGACTTGTTTTGGTGAAATCTGGACTATCAAAGCAGGCCGTGGGAAAGCTGGAAGTATCAGTTAAAAAGGATGCACAGAGTGGTTTGCAAGCTTGGATGTCAGCCCTCTCTGCTGTTGTCAGAGTGAATGAAAGTAGTTATAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

841

Amino Acids

94.61

Weight (kDa)

7.93

Isoelectric Point (pI)

37.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	299 - 344	6.4e-12	PPR repeat family
PPR_3	PF13812	300 - 346	2.4e-09	Pentatricopeptide repeat domain
PPR	PF01535	301 - 330	2e-06	PPR repeat
PPR_2	PF13041	332 - 378	2.5e-12	PPR repeat family
PPR_2	PF13041	367 - 414	8.7e-09	PPR repeat family
PPR_2	PF13041	402 - 447	1.4e-06	PPR repeat family
TPR_24	PF23276	495 - 603	3.4e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	504 - 551	2.9e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	506 - 553	1.4e-08	PPR repeat family
PPR_long	PF17177	522 - 629	2e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	580 - 623	1.4e-06	PPR repeat family
PPR_2	PF13041	750 - 792	1e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23020
fragaria_vesca	FvH4_4g02020
malus_domestica	MD13G1203500.v1.1 MD16G1205100.v1.1
prunus_persica	Prupe.1G026500_v2.0.a1
pyrus_communis	pycom13g17680
rosa_chinensis	RchiOBHm_Chr4g0388991 RchiOBHm_Chr4g0389031 RchiOBHm_Chr4g0389081 RchiOBHm_Chr4g0389161 RchiOBHm_Chr4g0389171
rosa_laevigata	RLG00000010001
rosa_multiflora	Rmu_sc0001395.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335330
rosa_rugosa	Rorug03G0321900
rosa_samantha	Rh4AG026400 Rh4BG019600 Rh4BG019800 Rh4BG019900 Rh4BG020200 Rh4BG020300 Rh4CG029400 Rh4DG020000 Rh4DG020400 Rh4DG020500 Rh4DG020900
rosa_wichuraiana	Rw4G001950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2262
Acc36I	ACCTGC	1 cut(s)	1913
AccIII	TCCGGA	1 cut(s)	1618
AciI	CCGC	4 cut(s)	410, 1222, 1680, 1787
AclWI	GGATC	1 cut(s)	1423
AcoI	YGGCCR	1 cut(s)	937
AcsI	RAATTY	1 cut(s)	1650
AcuI	CTGAAG	4 cut(s)	464, 1290, 1715, 1890
AcvI	CACGTG	1 cut(s)	357
AdeI	CACNNNGTG	3 cut(s)	130, 1258, 2456
AfaI	GTAC	3 cut(s)	1346, 1568, 1722
AfeI	AGCGCT	1 cut(s)	732
AfiI	CCNNNNNNNGG	7 cut(s)	43, 44, 71, 85, 114, 185, 253
AflIII	ACRYGT	1 cut(s)	356
AjnI	CCWGG	1 cut(s)	1930
AleI	CACNNNNGTG	1 cut(s)	1029
Alw21I	GWGCWC	4 cut(s)	135, 896, 1278, 2112
Alw26I	GTCTC	1 cut(s)	613
AlwI	GGATC	1 cut(s)	1423
AlwNI	CAGNNNCTG	2 cut(s)	1211, 1475
Aor13HI	TCCGGA	1 cut(s)	1618
Aor51HI	AGCGCT	1 cut(s)	732
AoxI	GGCC	6 cut(s)	937, 1685, 1981, 2022, 2202, 2412
ApeKI	GCWGC	5 cut(s)	742, 799, 1430, 1494, 1825
ApoI	RAATTY	1 cut(s)	1650
ArsI	GACNNNNNNTTYG	2 cut(s)	343, 375
Asp700I	GAANNNNTTC	1 cut(s)	1086
AspLEI	GCGC	1 cut(s)	733
AspS9I	GGNCC	1 cut(s)	2036
AsuHPI	GGTGA	7 cut(s)	54, 176, 349, 523, 1270, 1676, 2401
AvaII	GGWCC	1 cut(s)	2036
BanII	GRGCYC	4 cut(s)	50, 896, 1278, 2112
BauI	CACGAG	1 cut(s)	1105
BbrPI	CACGTG	1 cut(s)	357
Bbv12I	GWGCWC	4 cut(s)	135, 896, 1278, 2112
BbvCI	CCTCAGC	1 cut(s)	837
BbvI	GCAGC	5 cut(s)	729, 786, 1417, 1481, 1837
BccI	CCATC	5 cut(s)	1072, 1282, 1801, 2027, 2158
BceAI	ACGGC	3 cut(s)	423, 433, 2399
BcgI	CGANNNNNNTGC	2 cut(s)	781, 815
BciT130I	CCWGG	1 cut(s)	1932
BclI	TGATCA	1 cut(s)	1539
BcoDI	GTCTC	1 cut(s)	613
BfaI	CTAG	3 cut(s)	1140, 1338, 1659
BfmI	CTRYAG	3 cut(s)	743, 800, 1633
BfoI	RGCGCY	1 cut(s)	734
BfuAI	ACCTGC	1 cut(s)	1913
BisI	GCNGC	5 cut(s)	743, 800, 1431, 1495, 1826
BlsI	GCNGC	5 cut(s)	744, 801, 1432, 1496, 1827
Bme1390I	CCNGG	1 cut(s)	1932
Bme18I	GGWCC	1 cut(s)	2036
BmgT120I	GGNCC	1 cut(s)	2036
BmiI	GGNNCC	2 cut(s)	49, 2317
BmrFI	CCNGG	1 cut(s)	1932
BmrI	ACTGGG	1 cut(s)	2384
BmsI	GCATC	9 cut(s)	962, 963, 1350, 1432, 1692, 1740, 1897, 2128, 2437
BmuI	ACTGGG	1 cut(s)	2384
BpmI	CTGGAG	1 cut(s)	107
Bpu10I	CCTNAGC	3 cut(s)	590, 837, 977
BpuEI	CTTGAG	3 cut(s)	1220, 1325, 1385
BsaAI	YACGTR	2 cut(s)	357, 903
BsaJI	CCNNGG	4 cut(s)	449, 1930, 1931, 2415
BsaWI	WCCGGW	1 cut(s)	1618
Bsc4I	CCNNNNNNNGG	7 cut(s)	43, 44, 71, 85, 114, 185, 253
Bse118I	RCCGGY	1 cut(s)	939
Bse1I	ACTGG	2 cut(s)	1582, 2379
Bse3DI	GCAATG	3 cut(s)	869, 1917, 2166
BseAI	TCCGGA	1 cut(s)	1618
BseBI	CCWGG	1 cut(s)	1932
BseDI	CCNNGG	4 cut(s)	449, 1930, 1931, 2415
BseLI	CCNNNNNNNGG	7 cut(s)	43, 44, 71, 85, 114, 185, 253
BseMI	GCAATG	3 cut(s)	869, 1917, 2166
BseMII	CTCAG	8 cut(s)	365, 455, 828, 1416, 1467, 1909, 2040, 2246
BseNI	ACTGG	2 cut(s)	1582, 2379
BseRI	GAGGAG	8 cut(s)	72, 156, 431, 470, 517, 643, 854, 2213
BseXI	GCAGC	5 cut(s)	729, 786, 1417, 1481, 1837
BseYI	CCCAGC	2 cut(s)	65, 1355
BsgI	GTGCAG	1 cut(s)	1844
BshFI	GGCC	6 cut(s)	939, 1687, 1983, 2024, 2204, 2414
BsiHKAI	GWGCWC	4 cut(s)	135, 896, 1278, 2112
BsiSI	CCGG	3 cut(s)	395, 940, 1619
BslFI	GGGAC	3 cut(s)	700, 1314, 2391
BslI	CCNNNNNNNGG	7 cut(s)	43, 44, 71, 85, 114, 185, 253
BsmAI	GTCTC	1 cut(s)	613
BsmFI	GGGAC	3 cut(s)	700, 1314, 2391
BsmI	GAATGC	4 cut(s)	390, 874, 2343, 2363
BsnI	GGCC	6 cut(s)	939, 1687, 1983, 2024, 2204, 2414
Bsp1286I	GDGCHC	5 cut(s)	50, 135, 896, 1278, 2112
Bsp13I	TCCGGA	1 cut(s)	1618
Bsp1407I	TGTACA	1 cut(s)	1344
Bsp143I	GATC	4 cut(s)	915, 1415, 1539, 2068
BspACI	CCGC	4 cut(s)	410, 1222, 1680, 1787
BspANI	GGCC	6 cut(s)	939, 1687, 1983, 2024, 2204, 2414
BspCNI	CTCAG	8 cut(s)	366, 456, 829, 1415, 1468, 1908, 2039, 2247
BspEI	TCCGGA	1 cut(s)	1618
BspHI	TCATGA	1 cut(s)	1386
BspLI	GGNNCC	2 cut(s)	49, 2317
BspMAI	CTGCAG	2 cut(s)	747, 804
BspMI	ACCTGC	1 cut(s)	1913
BspPI	GGATC	1 cut(s)	1423
BspQI	GCTCTTC	3 cut(s)	1266, 2100, 2120
BsrDI	GCAATG	3 cut(s)	869, 1917, 2166
BsrFI	RCCGGY	1 cut(s)	939
BsrGI	TGTACA	1 cut(s)	1344
BsrI	ACTGG	2 cut(s)	1582, 2379
BssAI	RCCGGY	1 cut(s)	939
BssECI	CCNNGG	4 cut(s)	449, 1930, 1931, 2415
BssMI	GATC	4 cut(s)	915, 1415, 1539, 2068
BssSI	CACGAG	1 cut(s)	1105
Bst2BI	CACGAG	1 cut(s)	1105
Bst2UI	CCWGG	1 cut(s)	1932
Bst4CI	ACNGT	5 cut(s)	383, 1003, 1566, 1571, 1942
Bst6I	CTCTTC	4 cut(s)	1077, 1266, 2100, 2120
BstAPI	GCANNNNNTGC	1 cut(s)	130
BstAUI	TGTACA	1 cut(s)	1344
BstBAI	YACGTR	2 cut(s)	357, 903
BstC8I	GCNNGC	9 cut(s)	6, 937, 941, 1195, 1579, 2081, 2341, 2412, 2466
BstDSI	CCRYGG	2 cut(s)	449, 2415
BstH2I	RGCGCY	1 cut(s)	734
BstHHI	GCGC	1 cut(s)	733
BstKTI	GATC	4 cut(s)	918, 1418, 1542, 2071
BstMAI	GTCTC	1 cut(s)	613
BstMBI	GATC	4 cut(s)	915, 1415, 1539, 2068
BstMWI	GCNNNNNNNGC	7 cut(s)	10, 130, 739, 891, 2048, 2127, 2486
BstNI	CCWGG	1 cut(s)	1932
BstSCI	CCNGG	1 cut(s)	1930
BstSFI	CTRYAG	3 cut(s)	743, 800, 1633
BstSNI	TACGTA	1 cut(s)	903
BstV1I	GCAGC	5 cut(s)	729, 786, 1417, 1481, 1837
BstX2I	RGATCY	1 cut(s)	1415
BstYI	RGATCY	1 cut(s)	1415
BsuRI	GGCC	6 cut(s)	939, 1687, 1983, 2024, 2204, 2414
BtgI	CCRYGG	2 cut(s)	449, 2415
BtgZI	GCGATG	2 cut(s)	413, 2098
BtsIMutI	CAGTG	4 cut(s)	440, 968, 1825, 2372
BveI	ACCTGC	1 cut(s)	1913
Cac8I	GCNNGC	9 cut(s)	6, 937, 941, 1195, 1579, 2081, 2341, 2412, 2466
CaiI	CAGNNNCTG	2 cut(s)	1211, 1475
CciI	TCATGA	1 cut(s)	1386
CfoI	GCGC	1 cut(s)	733
Cfr10I	RCCGGY	1 cut(s)	939
Cfr13I	GGNCC	1 cut(s)	2036
Csp6I	GTAC	3 cut(s)	1345, 1567, 1721
CviQI	GTAC	3 cut(s)	1345, 1567, 1721
DpnI	GATC	4 cut(s)	917, 1417, 1541, 2070
DpnII	GATC	4 cut(s)	915, 1415, 1539, 2068
DraIII	CACNNNGTG	3 cut(s)	130, 1258, 2456
EaeI	YGGCCR	1 cut(s)	937
Eam1104I	CTCTTC	4 cut(s)	1077, 1266, 2100, 2120
EarI	CTCTTC	4 cut(s)	1077, 1266, 2100, 2120
EciI	GGCGGA	1 cut(s)	1802
Ecl136II	GAGCTC	3 cut(s)	894, 1276, 2110
Eco105I	TACGTA	1 cut(s)	903
Eco147I	AGGCCT	1 cut(s)	1687
Eco24I	GRGCYC	4 cut(s)	50, 896, 1278, 2112
Eco32I	GATATC	1 cut(s)	1752
Eco47I	GGWCC	1 cut(s)	2036
Eco47III	AGCGCT	1 cut(s)	732
Eco53kI	GAGCTC	3 cut(s)	894, 1276, 2110
Eco57I	CTGAAG	4 cut(s)	464, 1290, 1715, 1890
Eco72I	CACGTG	1 cut(s)	357
EcoICRI	GAGCTC	3 cut(s)	894, 1276, 2110
EcoRII	CCWGG	1 cut(s)	1930
EcoRV	GATATC	1 cut(s)	1752
EcoT22I	ATGCAT	3 cut(s)	1240, 1447, 1707
EcoT38I	GRGCYC	4 cut(s)	50, 896, 1278, 2112
FalI	AAGNNNNNCTT	4 cut(s)	717, 749, 1968, 2000
FaqI	GGGAC	3 cut(s)	700, 1314, 2391
FauNDI	CATATG	1 cut(s)	2290
FbaI	TGATCA	1 cut(s)	1539
Fnu4HI	GCNGC	5 cut(s)	743, 800, 1431, 1495, 1826
FriOI	GRGCYC	4 cut(s)	50, 896, 1278, 2112
Fsp4HI	GCNGC	5 cut(s)	743, 800, 1431, 1495, 1826
FspBI	CTAG	3 cut(s)	1140, 1338, 1659
GlaI	GCGC	1 cut(s)	732
GluI	GCNGC	5 cut(s)	743, 800, 1431, 1495, 1826
GsaI	CCCAGC	2 cut(s)	69, 1359
GsuI	CTGGAG	1 cut(s)	107
HaeII	RGCGCY	1 cut(s)	734
HaeIII	GGCC	6 cut(s)	939, 1687, 1983, 2024, 2204, 2414
HapII	CCGG	3 cut(s)	395, 940, 1619
HhaI	GCGC	1 cut(s)	733
Hin6I	GCGC	1 cut(s)	731
HinP1I	GCGC	1 cut(s)	731
HincII	GTYRAC	1 cut(s)	405
HindII	GTYRAC	1 cut(s)	405
HindIII	AAGCTT	2 cut(s)	1756, 2466
HinfI	GANTC	8 cut(s)	75, 571, 595, 1021, 1061, 1709, 1815, 2298
HpaII	CCGG	3 cut(s)	395, 940, 1619
HphI	GGTGA	7 cut(s)	54, 176, 349, 523, 1270, 1676, 2401
Hpy166II	GTNNAC	5 cut(s)	405, 1097, 1320, 1507, 2151
Hpy188III	TCNNGA	6 cut(s)	86, 151, 850, 1387, 1619, 2397
Hpy8I	GTNNAC	5 cut(s)	405, 1097, 1320, 1507, 2151
Hpy99I	CGWCG	2 cut(s)	367, 682
HpyAV	CCTTC	8 cut(s)	148, 295, 487, 598, 977, 1180, 2329, 2329
HpyCH4III	ACNGT	5 cut(s)	383, 1003, 1566, 1571, 1942
HpyCH4IV	ACGT	5 cut(s)	356, 680, 902, 1112, 1533
HpyF10VI	GCNNNNNNNGC	7 cut(s)	10, 130, 739, 891, 2048, 2127, 2486
HpySE526I	ACGT	5 cut(s)	356, 680, 902, 1112, 1533
HspAI	GCGC	1 cut(s)	731
Kpn2I	TCCGGA	1 cut(s)	1618
KroI	GCCGGC	1 cut(s)	939
KroNI	GCCGGC	1 cut(s)	941
Ksp22I	TGATCA	1 cut(s)	1539
Kzo9I	GATC	4 cut(s)	915, 1415, 1539, 2068
LguI	GCTCTTC	3 cut(s)	1266, 2100, 2120
LmnI	GCTCC	5 cut(s)	18, 53, 480, 496, 997
Lsp1109I	GCAGC	5 cut(s)	729, 786, 1417, 1481, 1837
LweI	GCATC	9 cut(s)	962, 963, 1350, 1432, 1692, 1740, 1897, 2128, 2437
MaeI	CTAG	3 cut(s)	1140, 1338, 1659
MaeII	ACGT	5 cut(s)	356, 680, 902, 1112, 1533
MaeIII	GTNAC	5 cut(s)	377, 467, 781, 1003, 1217
MalI	GATC	4 cut(s)	917, 1417, 1541, 2070
MboI	GATC	4 cut(s)	915, 1415, 1539, 2068
MfeI	CAATTG	1 cut(s)	1775
MflI	RGATCY	1 cut(s)	1415
MhlI	GDGCHC	5 cut(s)	50, 135, 896, 1278, 2112
MluCI	AATT	7 cut(s)	486, 1489, 1614, 1650, 1766, 1775, 2056
MlyI	GAGTC	2 cut(s)	69, 1718
MmeI	TCCRAC	3 cut(s)	1019, 1792, 1855
Mph1103I	ATGCAT	3 cut(s)	1240, 1447, 1707
MroI	TCCGGA	1 cut(s)	1618
MroNI	GCCGGC	1 cut(s)	939
MroXI	GAANNNNTTC	1 cut(s)	1086
MseI	TTAA	8 cut(s)	540, 945, 1488, 1884, 1958, 2094, 2243, 2439
MslI	CAYNNNNRTG	2 cut(s)	879, 1029
MspA1I	CMGCKG	1 cut(s)	19
MspI	CCGG	3 cut(s)	395, 940, 1619
MspR9I	CCNGG	1 cut(s)	1932
MunI	CAATTG	1 cut(s)	1775
Mva1269I	GAATGC	4 cut(s)	390, 874, 2343, 2363
MvaI	CCWGG	1 cut(s)	1932
MwoI	GCNNNNNNNGC	7 cut(s)	10, 130, 739, 891, 2048, 2127, 2486
NaeI	GCCGGC	1 cut(s)	941
NdeI	CATATG	1 cut(s)	2290
NdeII	GATC	4 cut(s)	915, 1415, 1539, 2068
NgoMIV	GCCGGC	1 cut(s)	939
NlaIV	GGNNCC	2 cut(s)	49, 2317
NmeAIII	GCCGAG	1 cut(s)	1523
NsiI	ATGCAT	3 cut(s)	1240, 1447, 1707
OliI	CACNNNNGTG	1 cut(s)	1029
PagI	TCATGA	1 cut(s)	1386
PasI	CCCWGGG	1 cut(s)	1931
PceI	AGGCCT	1 cut(s)	1687
PciSI	GCTCTTC	3 cut(s)	1266, 2100, 2120
PcsI	WCGNNNNNNNCGW	1 cut(s)	362
PctI	GAATGC	4 cut(s)	390, 874, 2343, 2363
PdiI	GCCGGC	1 cut(s)	941
PdmI	GAANNNNTTC	1 cut(s)	1086
PfeI	GAWTC	6 cut(s)	571, 595, 1021, 1061, 1815, 2298
PkrI	GCNGC	5 cut(s)	744, 801, 1432, 1496, 1827
PleI	GAGTC	2 cut(s)	69, 1717
PmaCI	CACGTG	1 cut(s)	357
PmlI	CACGTG	1 cut(s)	357
PpsI	GAGTC	2 cut(s)	69, 1717
Ppu21I	YACGTR	2 cut(s)	357, 903
PsiI	TTATAA	1 cut(s)	2262
Psp124BI	GAGCTC	3 cut(s)	896, 1278, 2112
Psp6I	CCWGG	1 cut(s)	1930
PspCI	CACGTG	1 cut(s)	357
PspFI	CCCAGC	2 cut(s)	65, 1355
PspGI	CCWGG	1 cut(s)	1930
PspN4I	GGNNCC	2 cut(s)	49, 2317
PspPI	GGNCC	1 cut(s)	2036
PsrI	GAACNNNNNNTAC	2 cut(s)	1621, 1653
PstI	CTGCAG	2 cut(s)	747, 804
PstNI	CAGNNNCTG	2 cut(s)	1211, 1475
PsuI	RGATCY	1 cut(s)	1415
PvuII	CAGCTG	1 cut(s)	19
RsaI	GTAC	3 cut(s)	1346, 1568, 1722
RsaNI	GTAC	3 cut(s)	1345, 1567, 1721
RseI	CAYNNNNRTG	2 cut(s)	879, 1029
SacI	GAGCTC	3 cut(s)	896, 1278, 2112
SapI	GCTCTTC	3 cut(s)	1266, 2100, 2120
SaqAI	TTAA	8 cut(s)	540, 945, 1488, 1884, 1958, 2094, 2243, 2439
SatI	GCNGC	5 cut(s)	743, 800, 1431, 1495, 1826
Sau3AI	GATC	4 cut(s)	915, 1415, 1539, 2068
Sau96I	GGNCC	1 cut(s)	2036
SchI	GAGTC	2 cut(s)	69, 1718
ScrFI	CCNGG	1 cut(s)	1932
SduI	GDGCHC	5 cut(s)	50, 135, 896, 1278, 2112
SfaNI	GCATC	9 cut(s)	962, 963, 1350, 1432, 1692, 1740, 1897, 2128, 2437
SfcI	CTRYAG	3 cut(s)	743, 800, 1633
SinI	GGWCC	1 cut(s)	2036
SmiMI	CAYNNNNRTG	2 cut(s)	879, 1029
SmlI	CTYRAG	3 cut(s)	1199, 1304, 1364
SmoI	CTYRAG	3 cut(s)	1199, 1304, 1364
SnaBI	TACGTA	1 cut(s)	903
Sse9I	AATT	7 cut(s)	486, 1489, 1614, 1650, 1766, 1775, 2056
SseBI	AGGCCT	1 cut(s)	1687
SsiI	CCGC	4 cut(s)	410, 1222, 1680, 1787
SspI	AATATT	2 cut(s)	1120, 1438
SspMI	CTAG	3 cut(s)	1140, 1338, 1659
SstI	GAGCTC	3 cut(s)	896, 1278, 2112
StuI	AGGCCT	1 cut(s)	1687
StyD4I	CCNGG	1 cut(s)	1930
TaaI	ACNGT	5 cut(s)	383, 1003, 1566, 1571, 1942
TaiI	ACGT	5 cut(s)	359, 683, 905, 1115, 1536
TaqI	TCGA	6 cut(s)	362, 531, 677, 849, 918, 1090
TasI	AATT	7 cut(s)	486, 1489, 1614, 1650, 1766, 1775, 2056
TatI	WGTACW	2 cut(s)	1344, 1566
TfiI	GAWTC	6 cut(s)	571, 595, 1021, 1061, 1815, 2298
Tru1I	TTAA	8 cut(s)	540, 945, 1488, 1884, 1958, 2094, 2243, 2439
Tru9I	TTAA	8 cut(s)	540, 945, 1488, 1884, 1958, 2094, 2243, 2439
TscAI	CASTG	4 cut(s)	447, 975, 1825, 2379
TseI	GCWGC	5 cut(s)	742, 799, 1430, 1494, 1825
TspGWI	ACGGA	2 cut(s)	153, 1464
TspRI	CASTG	4 cut(s)	447, 975, 1825, 2379
VpaK11BI	GGWCC	1 cut(s)	2036
XapI	RAATTY	1 cut(s)	1650
XcmI	CCANNNNNNNNNTGG	1 cut(s)	16
XmnI	GAANNNNTTC	1 cut(s)	1086
XspI	CTAG	3 cut(s)	1140, 1338, 1659
Zsp2I	ATGCAT	3 cut(s)	1240, 1447, 1707

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.