Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0006344

Physical Location & Seq

Genomic Coordinates Forward (+)

61610741 .. 61613263

Gene Structure 2523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010001

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGCTTACGAAGCTCCAGATGGACTTCCCCTTTTTGGGTTCCACAAGGACCTCACCCAACACAGGACTCCCTCCACTGGAGAAACTCGAACTCATAACCAGAGATAGGAAGCAGAGAGTGCTTCACGGAGGAGGGTTCAAGAAGGGTTTTGGTGAAGTTTGCAGACCCAGACACAGTAATGGTGGCAGTGTTGTTGTTCATGAAGTTGGGTTGAAGAAGCGTGGTTTTGGGAAGAACCCAGATGAGGAAAAGGGATTGGAGAATCTGGGTGGTGGTGGGTTTGTGGAAAGAAAGGATTTTGGGGTTGAATTGGGGTTATGGGGTGGGAATGGTGTGGTGAAGAAAGTGCAGACCAAGTGTTCGACGAAATGGCTGAGTTACGGTGGGTGCATTCCGGCAATGTTGACGGCGTTGGAGGAAGTTGAGGACTTGGATGAGGCTCTGAAGCCGTGGGGAGAGACGCTTAGTAATAAGGAAAGGAGCATAATTTTGAAGGAGCAGAGGAGTTGGGAAAGGGCTTTGGAGATTTTCGAGTGGTTTAAGAAGAAGAGGTGTTATGAGTTGCATGTGATTCACTATAACATAGTGCTGAGAATTCTGGGGAAGGCGAAGAAGTGGATTCATCTAAGGAGTCTTTGGGATGAGATGAATGTGGAAAGAATAGCACCCATTAACTCAACTTATGGGACTTTGATTGATGTTTACAGTAAAGGTGGGTTCGAAAAGGAAGCGCTTATTTGGCTGCAGAGGATGACCAAGCAAGGAATGAAACCTGATGAGGTTACTATGGCCATTGTGCTGCAGTTGTATAAGAAAGCAGGGGAGTACCGAAAAGCGGAGGAGTTTTTCGAGAAATGGTCAGAGAGCTCGTTGCATTGTCATGGTTCTTTGAGCTCACATACGTATAACACATTGATCGACACGCATGGAAAGGCTGGCCAGCTTAAAGAAGCATCGGAAATATTTGCATCGATGCTAAGGGAAGGGATTGCTCCAACTACAGTGACTTTCAATACAATGATTCACATTTGTGGTAATCATGGTCAGCTTGCAGAAGTTGCTTCACTTATGCAGAAGATGGAAGAGGTTCGATGCCCACCAGACACGAGAACATATAATATTCTGATTTCCCTCCATGCTAGGCACGATAATATAGACATGGCTACAAACTACTTCGTAAAGATGAAGGAGGCTTGCCTTGAGCCTGACCCTGTGAGTTATCGCACCCTTTTGTATGCATACTCTGTAAGGCACATGGTTAGCAAAGCTGAAGGCCTCATATCGGAGATGGATGAAAGGGGACTTGAGATTGATGAGTTCACTCAGTCGGCTCTGACTAGGATGTACATAGAAGCGGGGATGCTTGAGAAGTCTCAGGTTTGGTTCATGAGATTTCATATTTCAGGGAAAATGGGATCTGATTGCTATTCTGCCAACATTGATGCATATGGAGAGCGTGGGCATATTTTGGAAGCTGAGAACGTTTTCAATTGCTGCCGAGAAGTGAAGAAGTTGAGTGTCCTTGAGTTCAACGTGATGATCAAAGCATATGGGGTTGGTAAACATTACAGTAGAGCTTGCCAATTGTTTGATAGCATGGAGAATCATGGCGTAATTCCGGACAGGTGTAGCTATAGTTCTCTGATACAAATTTTGGCTAGTGGTGATGTGCCACATACGGCAATGCCTTATCTGAAGAAGATGCAGGAGTCAGGTTTGGTACGTGATTGCATCCCCTATTGTGCTGTGATATCAAGCTTTGCAAAATTAGGTCAATTGGAAAGGGCAGAAGACATTTATAAACAGATGGTTGGATTCAATGTGCAGCCTGATGTAATTGTCTTTGGGGTGTTGATAAATGCTTTTGCTGAAGTTGGAAGCGTTAAAGAAGCTCTCGGTTATGCTGATGCAATGAAAAAGGCAGGTTTCCCTGGGAACACAGTTGTTTACAATACCTTGATCAAGCTCTATACAAAAGTTGGCCTTTTGAAAGAAGCAGAAGAAACGTACAAACTTCTTCAGTCATCAGAGGATGGTCCTGCTATATATGCGTCAAATTGTATGATTGATCTTTATAGCGAGCGATGTATGGTTAAACCAGCAGAAGAACTCTTTGACAGCTTGAAGAGAAAGGGGGATGCAAATGAGTTTACATTTGCAATGATGTTGTGCATGTATAAGAGAATTGGGAGGTTTGAGGAAGCCATTCAGATTGCAGAGCAGATGAGAGAACTGCGACTTTTAACTGATCTGCTGAGTTATAATAATGTTATTGGGTTGTATGCGACATATGGAAGATTCAAAGAGGTGGTGGGAACTTTGAAGGAAATGACAAAAGCTGCTATTCAGCCTGATGAATGCACATTCAAATCACTAGGACTTGTTTTGGTGAAATCTGGACTTTCAAAGCAGGCTGTGGGAAAACTGGAAGTATCGGTTAAGAAGGATGCTCTGAGTGGTTTGCAAGCTTGGATGTCGGCCCTCTCTGCTGTTGTGAGAGTGAATGAAAGTAGTTATATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

841

Amino Acids

94.67

Weight (kDa)

7.74

Isoelectric Point (pI)

38.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	296 - 391	2.1e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	299 - 342	1.9e-12	PPR repeat family
PPR_3	PF13812	300 - 346	1.4e-09	Pentatricopeptide repeat domain
PPR	PF01535	301 - 330	2.1e-06	PPR repeat
PPR_long	PF17177	302 - 457	4.6e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	332 - 378	4.9e-12	PPR repeat family
PPR_2	PF13041	367 - 414	2.2e-08	PPR repeat family
TPR_24	PF23276	499 - 603	6.7e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	510 - 553	1e-07	PPR repeat family
PPR_long	PF17177	522 - 675	2.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	580 - 623	2.4e-06	PPR repeat family
PPR_2	PF13041	751 - 792	2.7e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23020
fragaria_vesca	FvH4_4g02020
malus_domestica	MD13G1203500.v1.1 MD16G1205100.v1.1
prunus_persica	Prupe.1G026500_v2.0.a1
pyrus_communis	pycom13g17680
rosa_chinensis	RchiOBHm_Chr4g0388991 RchiOBHm_Chr4g0389031 RchiOBHm_Chr4g0389081 RchiOBHm_Chr4g0389161 RchiOBHm_Chr4g0389171
rosa_laevigata	RLG00000010001
rosa_multiflora	Rmu_sc0001395.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335330
rosa_rugosa	Rorug03G0321900
rosa_samantha	Rh4AG026400 Rh4BG019600 Rh4BG019800 Rh4BG019900 Rh4BG020200 Rh4BG020300 Rh4CG029400 Rh4DG020000 Rh4DG020400 Rh4DG020500 Rh4DG020900
rosa_wichuraiana	Rw4G001950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1800, 2262
Acc36I	ACCTGC	1 cut(s)	1913
AccIII	TCCGGA	1 cut(s)	1618
AciI	CCGC	2 cut(s)	836, 1355
AclI	AACGTT	1 cut(s)	1482
AclWI	GGATC	1 cut(s)	1423
AcoI	YGGCCR	2 cut(s)	789, 937
AcsI	RAATTY	2 cut(s)	594, 1650
AcuI	CTGAAG	5 cut(s)	464, 1290, 1715, 1890, 2003
AfaI	GTAC	4 cut(s)	827, 1346, 1722, 2009
AfeI	AGCGCT	1 cut(s)	732
AfiI	CCNNNNNNNGG	8 cut(s)	34, 35, 62, 76, 105, 244, 835, 1282
AjnI	CCWGG	1 cut(s)	1930
AleI	CACNNNNGTG	1 cut(s)	1029
Alw21I	GWGCWC	2 cut(s)	869, 896
Alw26I	GTCTC	2 cut(s)	452, 1377
AlwI	GGATC	1 cut(s)	1423
Aor13HI	TCCGGA	1 cut(s)	1618
Aor51HI	AGCGCT	1 cut(s)	732
AoxI	GGCC	5 cut(s)	789, 937, 1273, 1981, 2478
ApeKI	GCWGC	5 cut(s)	742, 799, 1494, 1825, 2339
ApoI	RAATTY	2 cut(s)	594, 1650
ArsI	GACNNNNNNTTYG	2 cut(s)	343, 375
Asp700I	GAANNNNTTC	3 cut(s)	1086, 1484, 2205
AspLEI	GCGC	1 cut(s)	733
AspS9I	GGNCC	3 cut(s)	48, 2036, 2479
AsuHPI	GGTGA	5 cut(s)	45, 164, 349, 1676, 2401
AsuII	TTCGAA	1 cut(s)	720
AvaII	GGWCC	2 cut(s)	48, 2036
BalI	TGGCCA	2 cut(s)	791, 939
BanII	GRGCYC	2 cut(s)	869, 896
BauI	CACGAG	1 cut(s)	1105
BbsI	GAAGAC	1 cut(s)	1797
Bbv12I	GWGCWC	2 cut(s)	869, 896
BbvI	GCAGC	5 cut(s)	729, 786, 1481, 1837, 2326
BccI	CCATC	5 cut(s)	13, 1072, 1282, 1801, 2027
BceAI	ACGGC	3 cut(s)	423, 433, 1695
BciT130I	CCWGG	1 cut(s)	1932
BclI	TGATCA	2 cut(s)	1539, 1959
BcoDI	GTCTC	2 cut(s)	452, 1377
BfaI	CTAG	4 cut(s)	1140, 1338, 1659, 2375
BfmI	CTRYAG	4 cut(s)	743, 800, 999, 1633
BfoI	RGCGCY	1 cut(s)	734
BfuAI	ACCTGC	1 cut(s)	1913
BisI	GCNGC	5 cut(s)	743, 800, 1495, 1826, 2340
BlsI	GCNGC	5 cut(s)	744, 801, 1496, 1827, 2341
Bme1390I	CCNGG	1 cut(s)	1932
Bme18I	GGWCC	2 cut(s)	48, 2036
BmgT120I	GGNCC	3 cut(s)	48, 2036, 2479
BmiI	GGNNCC	1 cut(s)	40
BmrFI	CCNGG	1 cut(s)	1932
BpiI	GAAGAC	1 cut(s)	1797
BpmI	CTGGAG	1 cut(s)	98
Bpu10I	CCTNAGC	1 cut(s)	977
Bpu14I	TTCGAA	1 cut(s)	720
BpuEI	CTTGAG	4 cut(s)	1220, 1325, 1385, 1544
Bsa29I	ATCGAT	1 cut(s)	971
BsaAI	YACGTR	2 cut(s)	903, 1724
BsaJI	CCNNGG	3 cut(s)	449, 1930, 1931
BsaWI	WCCGGW	1 cut(s)	1618
Bsc4I	CCNNNNNNNGG	8 cut(s)	34, 35, 62, 76, 105, 244, 835, 1282
Bse1I	ACTGG	2 cut(s)	81, 2430
Bse3DI	GCAATG	4 cut(s)	405, 1689, 1917, 2166
BseAI	TCCGGA	1 cut(s)	1618
BseBI	CCWGG	1 cut(s)	1932
BseCI	ATCGAT	1 cut(s)	971
BseDI	CCNNGG	3 cut(s)	449, 1930, 1931
BseLI	CCNNNNNNNGG	8 cut(s)	34, 35, 62, 76, 105, 244, 835, 1282
BseMI	GCAATG	4 cut(s)	405, 1689, 1917, 2166
BseMII	CTCAG	7 cut(s)	365, 581, 1337, 1388, 1467, 2246, 2444
BseNI	ACTGG	2 cut(s)	81, 2430
BseRI	GAGGAG	3 cut(s)	144, 517, 854
BseXI	GCAGC	5 cut(s)	729, 786, 1481, 1837, 2326
BsgI	GTGCAG	2 cut(s)	368, 1844
BshFI	GGCC	5 cut(s)	791, 939, 1275, 1983, 2480
BshVI	ATCGAT	1 cut(s)	971
BsiHKAI	GWGCWC	2 cut(s)	869, 896
BsiSI	CCGG	2 cut(s)	395, 1619
BslFI	GGGAC	2 cut(s)	700, 1314
BslI	CCNNNNNNNGG	8 cut(s)	34, 35, 62, 76, 105, 244, 835, 1282
BsmAI	GTCTC	2 cut(s)	452, 1377
BsmBI	CGTCTC	1 cut(s)	452
BsmFI	GGGAC	2 cut(s)	700, 1314
BsmI	GAATGC	2 cut(s)	390, 2363
BsnI	GGCC	5 cut(s)	791, 939, 1275, 1983, 2480
Bsp119I	TTCGAA	1 cut(s)	720
Bsp1286I	GDGCHC	2 cut(s)	869, 896
Bsp13I	TCCGGA	1 cut(s)	1618
Bsp1407I	TGTACA	1 cut(s)	1344
Bsp143I	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
BspACI	CCGC	2 cut(s)	836, 1355
BspANI	GGCC	5 cut(s)	791, 939, 1275, 1983, 2480
BspCNI	CTCAG	7 cut(s)	366, 582, 1336, 1387, 1468, 2247, 2445
BspDI	ATCGAT	1 cut(s)	971
BspEI	TCCGGA	1 cut(s)	1618
BspHI	TCATGA	2 cut(s)	199, 1386
BspLI	GGNNCC	1 cut(s)	40
BspMAI	CTGCAG	2 cut(s)	747, 804
BspMI	ACCTGC	1 cut(s)	1913
BspPI	GGATC	1 cut(s)	1423
BspT104I	TTCGAA	1 cut(s)	720
BsrDI	GCAATG	4 cut(s)	405, 1689, 1917, 2166
BsrGI	TGTACA	1 cut(s)	1344
BsrI	ACTGG	2 cut(s)	81, 2430
BssECI	CCNNGG	3 cut(s)	449, 1930, 1931
BssMI	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
BssSI	CACGAG	1 cut(s)	1105
Bst2BI	CACGAG	1 cut(s)	1105
Bst2UI	CCWGG	1 cut(s)	1932
Bst4CI	ACNGT	6 cut(s)	176, 383, 707, 1003, 1571, 1942
Bst6I	CTCTTC	3 cut(s)	542, 1077, 2120
BstAPI	GCANNNNNTGC	2 cut(s)	118, 1058
BstAUI	TGTACA	1 cut(s)	1344
BstBAI	YACGTR	2 cut(s)	903, 1724
BstBI	TTCGAA	1 cut(s)	720
BstC8I	GCNNGC	8 cut(s)	937, 941, 1050, 1195, 1579, 2081, 2412, 2466
BstDSI	CCRYGG	1 cut(s)	449
BstH2I	RGCGCY	1 cut(s)	734
BstHHI	GCGC	1 cut(s)	733
BstKTI	GATC	6 cut(s)	918, 1418, 1542, 1962, 2071, 2251
BstMAI	GTCTC	2 cut(s)	452, 1377
BstMBI	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
BstMWI	GCNNNNNNNGC	6 cut(s)	10, 118, 739, 1058, 2048, 2486
BstNI	CCWGG	1 cut(s)	1932
BstNSI	RCATGY	2 cut(s)	569, 2176
BstSCI	CCNGG	1 cut(s)	1930
BstSFI	CTRYAG	4 cut(s)	743, 800, 999, 1633
BstSNI	TACGTA	1 cut(s)	903
BstV1I	GCAGC	5 cut(s)	729, 786, 1481, 1837, 2326
BstV2I	GAAGAC	1 cut(s)	1797
BstX2I	RGATCY	1 cut(s)	1415
BstYI	RGATCY	1 cut(s)	1415
Bsu15I	ATCGAT	1 cut(s)	971
BsuRI	GGCC	5 cut(s)	791, 939, 1275, 1983, 2480
BsuTUI	ATCGAT	1 cut(s)	971
BtgI	CCRYGG	1 cut(s)	449
BtgZI	GCGATG	1 cut(s)	2098
BtsI	GCAGTG	1 cut(s)	193
BtsIMutI	CAGTG	3 cut(s)	74, 193, 1008
BveI	ACCTGC	1 cut(s)	1913
Cac8I	GCNNGC	8 cut(s)	937, 941, 1050, 1195, 1579, 2081, 2412, 2466
CciI	TCATGA	2 cut(s)	199, 1386
CfoI	GCGC	1 cut(s)	733
Cfr13I	GGNCC	3 cut(s)	48, 2036, 2479
ClaI	ATCGAT	1 cut(s)	971
CseI	GACGC	2 cut(s)	469, 2040
Csp6I	GTAC	4 cut(s)	826, 1345, 1721, 2008
CviQI	GTAC	4 cut(s)	826, 1345, 1721, 2008
DpnI	GATC	6 cut(s)	917, 1417, 1541, 1961, 2070, 2250
DpnII	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
EaeI	YGGCCR	2 cut(s)	789, 937
Eam1104I	CTCTTC	3 cut(s)	542, 1077, 2120
EarI	CTCTTC	3 cut(s)	542, 1077, 2120
Ecl136II	GAGCTC	2 cut(s)	867, 894
Eco105I	TACGTA	1 cut(s)	903
Eco147I	AGGCCT	1 cut(s)	1275
Eco24I	GRGCYC	2 cut(s)	869, 896
Eco32I	GATATC	1 cut(s)	1752
Eco47I	GGWCC	2 cut(s)	48, 2036
Eco47III	AGCGCT	1 cut(s)	732
Eco53kI	GAGCTC	2 cut(s)	867, 894
Eco57I	CTGAAG	5 cut(s)	464, 1290, 1715, 1890, 2003
EcoICRI	GAGCTC	2 cut(s)	867, 894
EcoO109I	RGGNCCY	1 cut(s)	48
EcoRI	GAATTC	1 cut(s)	594
EcoRII	CCWGG	1 cut(s)	1930
EcoRV	GATATC	1 cut(s)	1752
EcoT22I	ATGCAT	2 cut(s)	1240, 1447
EcoT38I	GRGCYC	2 cut(s)	869, 896
Esp3I	CGTCTC	1 cut(s)	452
FalI	AAGNNNNNCTT	2 cut(s)	1968, 2000
FaqI	GGGAC	2 cut(s)	700, 1314
FauI	CCCGC	1 cut(s)	1348
FauNDI	CATATG	3 cut(s)	1447, 1549, 2290
FbaI	TGATCA	2 cut(s)	1539, 1959
Fnu4HI	GCNGC	5 cut(s)	743, 800, 1495, 1826, 2340
FriOI	GRGCYC	2 cut(s)	869, 896
Fsp4HI	GCNGC	5 cut(s)	743, 800, 1495, 1826, 2340
FspBI	CTAG	4 cut(s)	1140, 1338, 1659, 2375
GlaI	GCGC	1 cut(s)	732
GluI	GCNGC	5 cut(s)	743, 800, 1495, 1826, 2340
GsuI	CTGGAG	1 cut(s)	98
HaeII	RGCGCY	1 cut(s)	734
HaeIII	GGCC	5 cut(s)	791, 939, 1275, 1983, 2480
HapII	CCGG	2 cut(s)	395, 1619
HgaI	GACGC	2 cut(s)	469, 2040
HhaI	GCGC	1 cut(s)	733
Hin6I	GCGC	1 cut(s)	731
HinP1I	GCGC	1 cut(s)	731
HincII	GTYRAC	1 cut(s)	405
HindII	GTYRAC	1 cut(s)	405
HindIII	AAGCTT	2 cut(s)	1756, 2466
HpaII	CCGG	2 cut(s)	395, 1619
HphI	GGTGA	5 cut(s)	45, 164, 349, 1676, 2401
Hpy166II	GTNNAC	6 cut(s)	405, 703, 1320, 1562, 1948, 2151
Hpy188III	TCNNGA	7 cut(s)	16, 139, 200, 850, 1387, 1619, 2397
Hpy8I	GTNNAC	6 cut(s)	405, 703, 1320, 1562, 1948, 2151
Hpy99I	CGWCG	1 cut(s)	367
HpyAV	CCTTC	8 cut(s)	136, 487, 598, 977, 1180, 1265, 2317, 2437
HpyCH4III	ACNGT	6 cut(s)	176, 383, 707, 1003, 1571, 1942
HpyCH4IV	ACGT	5 cut(s)	902, 1482, 1533, 1723, 2006
HpyF10VI	GCNNNNNNNGC	6 cut(s)	10, 118, 739, 1058, 2048, 2486
HpySE526I	ACGT	5 cut(s)	902, 1482, 1533, 1723, 2006
HspAI	GCGC	1 cut(s)	731
Kpn2I	TCCGGA	1 cut(s)	1618
Ksp22I	TGATCA	2 cut(s)	1539, 1959
Kzo9I	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
LmnI	GCTCC	4 cut(s)	18, 480, 496, 997
Lsp1109I	GCAGC	5 cut(s)	729, 786, 1481, 1837, 2326
MaeI	CTAG	4 cut(s)	1140, 1338, 1659, 2375
MaeII	ACGT	5 cut(s)	902, 1482, 1533, 1723, 2006
MaeIII	GTNAC	3 cut(s)	377, 781, 1003
MalI	GATC	6 cut(s)	917, 1417, 1541, 1961, 2070, 2250
MboI	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
MfeI	CAATTG	3 cut(s)	1489, 1583, 1775
MflI	RGATCY	1 cut(s)	1415
MhlI	GDGCHC	2 cut(s)	869, 896
MlsI	TGGCCA	2 cut(s)	791, 939
MluNI	TGGCCA	2 cut(s)	791, 939
MlyI	GAGTC	3 cut(s)	60, 640, 1718
MmeI	TCCRAC	4 cut(s)	393, 1019, 1792, 1855
Mox20I	TGGCCA	2 cut(s)	791, 939
Mph1103I	ATGCAT	2 cut(s)	1240, 1447
MroI	TCCGGA	1 cut(s)	1618
MroXI	GAANNNNTTC	3 cut(s)	1086, 1484, 2205
MscI	TGGCCA	2 cut(s)	791, 939
MseI	TTAA	7 cut(s)	540, 672, 945, 1884, 2094, 2243, 2439
MslI	CAYNNNNRTG	3 cut(s)	177, 879, 1029
Msp20I	TGGCCA	2 cut(s)	791, 939
MspI	CCGG	2 cut(s)	395, 1619
MspR9I	CCNGG	1 cut(s)	1932
MunI	CAATTG	3 cut(s)	1489, 1583, 1775
Mva1269I	GAATGC	2 cut(s)	390, 2363
MvaI	CCWGG	1 cut(s)	1932
MwoI	GCNNNNNNNGC	6 cut(s)	10, 118, 739, 1058, 2048, 2486
NdeI	CATATG	3 cut(s)	1447, 1549, 2290
NdeII	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
NlaIV	GGNNCC	1 cut(s)	40
NmeAIII	GCCGAG	1 cut(s)	1523
NmuCI	GTSAC	1 cut(s)	1003
NsiI	ATGCAT	2 cut(s)	1240, 1447
NspI	RCATGY	2 cut(s)	569, 2176
NspV	TTCGAA	1 cut(s)	720
OliI	CACNNNNGTG	1 cut(s)	1029
PagI	TCATGA	2 cut(s)	199, 1386
PasI	CCCWGGG	1 cut(s)	1931
PceI	AGGCCT	1 cut(s)	1275
PctI	GAATGC	2 cut(s)	390, 2363
PdmI	GAANNNNTTC	3 cut(s)	1086, 1484, 2205
PfeI	GAWTC	7 cut(s)	262, 571, 619, 1021, 1603, 1815, 2298
PkrI	GCNGC	5 cut(s)	744, 801, 1496, 1827, 2341
PleI	GAGTC	3 cut(s)	60, 639, 1717
PpsI	GAGTC	3 cut(s)	60, 639, 1717
Ppu21I	YACGTR	2 cut(s)	903, 1724
PpuMI	RGGWCCY	1 cut(s)	48
PsiI	TTATAA	2 cut(s)	1800, 2262
Psp124BI	GAGCTC	2 cut(s)	869, 896
Psp1406I	AACGTT	1 cut(s)	1482
Psp5II	RGGWCCY	1 cut(s)	48
Psp6I	CCWGG	1 cut(s)	1930
PspGI	CCWGG	1 cut(s)	1930
PspN4I	GGNNCC	1 cut(s)	40
PspPI	GGNCC	3 cut(s)	48, 2036, 2479
PspPPI	RGGWCCY	1 cut(s)	48
PsrI	GAACNNNNNNTAC	2 cut(s)	1621, 1653
PstI	CTGCAG	2 cut(s)	747, 804
PsuI	RGATCY	1 cut(s)	1415
RsaI	GTAC	4 cut(s)	827, 1346, 1722, 2009
RsaNI	GTAC	4 cut(s)	826, 1345, 1721, 2008
RseI	CAYNNNNRTG	3 cut(s)	177, 879, 1029
SacI	GAGCTC	2 cut(s)	869, 896
SaqAI	TTAA	7 cut(s)	540, 672, 945, 1884, 2094, 2243, 2439
SatI	GCNGC	5 cut(s)	743, 800, 1495, 1826, 2340
Sau3AI	GATC	6 cut(s)	915, 1415, 1539, 1959, 2068, 2248
Sau96I	GGNCC	3 cut(s)	48, 2036, 2479
SchI	GAGTC	3 cut(s)	60, 640, 1718
ScrFI	CCNGG	1 cut(s)	1932
SduI	GDGCHC	2 cut(s)	869, 896
SfcI	CTRYAG	4 cut(s)	743, 800, 999, 1633
SfuI	TTCGAA	1 cut(s)	720
SinI	GGWCC	2 cut(s)	48, 2036
SmiMI	CAYNNNNRTG	3 cut(s)	177, 879, 1029
SmlI	CTYRAG	4 cut(s)	1199, 1304, 1364, 1523
SmoI	CTYRAG	4 cut(s)	1199, 1304, 1364, 1523
SnaBI	TACGTA	1 cut(s)	903
SseBI	AGGCCT	1 cut(s)	1275
SsiI	CCGC	2 cut(s)	836, 1355
SspI	AATATT	2 cut(s)	963, 1120
SspMI	CTAG	4 cut(s)	1140, 1338, 1659, 2375
SstI	GAGCTC	2 cut(s)	869, 896
StuI	AGGCCT	1 cut(s)	1275
StyD4I	CCNGG	1 cut(s)	1930
TaaI	ACNGT	6 cut(s)	176, 383, 707, 1003, 1571, 1942
TaiI	ACGT	5 cut(s)	905, 1485, 1536, 1726, 2009
TaqI	TCGA	8 cut(s)	87, 362, 531, 720, 849, 918, 971, 1090
TatI	WGTACW	1 cut(s)	1344
TfiI	GAWTC	7 cut(s)	262, 571, 619, 1021, 1603, 1815, 2298
Tru1I	TTAA	7 cut(s)	540, 672, 945, 1884, 2094, 2243, 2439
Tru9I	TTAA	7 cut(s)	540, 672, 945, 1884, 2094, 2243, 2439
TscAI	CASTG	3 cut(s)	81, 193, 1008
TseFI	GTSAC	1 cut(s)	1003
TseI	GCWGC	5 cut(s)	742, 799, 1494, 1825, 2339
Tsp45I	GTSAC	1 cut(s)	1003
TspGWI	ACGGA	1 cut(s)	141
TspRI	CASTG	3 cut(s)	81, 193, 1008
VpaK11BI	GGWCC	2 cut(s)	48, 2036
XapI	RAATTY	2 cut(s)	594, 1650
XceI	RCATGY	2 cut(s)	569, 2176
XmnI	GAANNNNTTC	3 cut(s)	1086, 1484, 2205
XspI	CTAG	4 cut(s)	1140, 1338, 1659, 2375
Zsp2I	ATGCAT	2 cut(s)	1240, 1447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.