Rh4DG020900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006344
Physical Location & Seq
Reverse (-)
3248769 .. 3249746
978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG020900.1

Sequence Viewer

Length: 978 bp
ATGCTTGCGAATCTCCAGTTGGACTTCCCCGTTTTGGGTTCCACAAGGACCTCACCCAACACAGGACTCCCTCCACTGGAGAAACTCGAACTCATAACCAGAGATAGGAAGCAGAGAGTGCTTCACGGAGGAGGGTTCAAGAAGGGTTTTGGTGAAGTTTGTAGACCCAGACACAGTAATGGTGGCAGTGTTGTTGTTCATGAAGTTGGGTTGAAGAAGCGTGGTTTTGTGAAGAACCCAGATGAGGAAAAGGGATTAGAGAATCTGGGTGATGGTGGGTTTGTGGAAAGAAAGGATTTTGGGGTTGAATTGGGGTTGGGGGGTGGGAATGGTGTGGTGAAGAAAGTGCAGACCAAGTGTTCGACGAAATGGCTGAGTTACGGTGGGTGCATTCCGGCAATGTTGACGGCGTTGGAGGAAGTTGAGGACGTGGATGAGGCTCTGAGGCCGTGGGGAGAGACGCTTAGTAATAAGGAAAGGAGCATAATTTTGAAGGAGCAGAGGAGTTGGGAAAGGGCTTTGGAGATTTTCGAGTGGTTTAAGAAGAAGAGGTGTTATGAGTTGCATGTGATTCACTATAATATAGTGCTTAGGATTCTTGGGAAGGCGAAGAAGTGGAGTCATCTAAGGAGTCTTTGGGATGAGATGAATGTGGAAAGAATAGCACCCATTAACTCAACTTATGGGACTTTGATTGATGTTTACAGTAAAGGTGGGTTCGAAAAGGAAGCGCTTATATGGCTGCAGAGGATGACCAAGCAAGGACTGAAACCTGATGAGGTTACTATGGCCATTGTGCTGCAGTTGTATAAGAAAGCAGGGGAGTACCGAAAAGCGGAGGAGTTTTTTGAGAAATGGTCGGAGAGCTCCTTGCATTGTCATGGTTCTTTGTGCTCGCATACGTATAACACATTGATCGACACGCGTGGAAAGGCTGGCCAGCTTAAGAAGCGTCGGAAATGTTTGCATCGATGCTGA

Protein Analysis

325

Amino Acids

37.01

Weight (kDa)

9.35

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 171 - 324 1.7e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 163
AccII CGCG 1 cut(s) 925
AciI CCGC 1 cut(s) 836
AcoI YGGCCR 2 cut(s) 789, 937
AfaI GTAC 1 cut(s) 827
AfeI AGCGCT 1 cut(s) 732
AfiI CCNNNNNNNGG 7 cut(s) 34, 35, 62, 76, 105, 244, 835
AflII CTTAAG 1 cut(s) 944
AflIII ACRYGT 1 cut(s) 923
AgsI TTSAA 4 cut(s) 139, 214, 308, 493
AjiI CACGTC 1 cut(s) 430
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 2 cut(s) 867, 943
AluI AGCT 2 cut(s) 867, 943
Alw21I GWGCWC 2 cut(s) 869, 896
Alw26I GTCTC 1 cut(s) 452
Aor51HI AGCGCT 1 cut(s) 732
AoxI GGCC 3 cut(s) 446, 789, 937
ApeKI GCWGC 2 cut(s) 742, 799
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AspLEI GCGC 1 cut(s) 733
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 4 cut(s) 45, 164, 281, 349
AsuII TTCGAA 1 cut(s) 720
AvaII GGWCC 1 cut(s) 48
BalI TGGCCA 2 cut(s) 791, 939
BanII GRGCYC 1 cut(s) 869
Bbv12I GWGCWC 2 cut(s) 869, 896
BbvI GCAGC 2 cut(s) 729, 786
BccI CCATC 1 cut(s) 266
BceAI ACGGC 2 cut(s) 423, 433
BcoDI GTCTC 1 cut(s) 452
BfmI CTRYAG 2 cut(s) 743, 800
BfoI RGCGCY 1 cut(s) 734
BfrI CTTAAG 1 cut(s) 944
BisI GCNGC 2 cut(s) 743, 800
BlsI GCNGC 2 cut(s) 744, 801
Bme18I GGWCC 1 cut(s) 48
BmgBI CACGTC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 1 cut(s) 40
BmsI GCATC 1 cut(s) 962
BpmI CTGGAG 1 cut(s) 98
Bpu10I CCTNAGC 1 cut(s) 590
Bpu14I TTCGAA 1 cut(s) 720
Bsa29I ATCGAT 1 cut(s) 970
BsaAI YACGTR 1 cut(s) 903
BsaJI CCNNGG 1 cut(s) 449
Bsc4I CCNNNNNNNGG 7 cut(s) 34, 35, 62, 76, 105, 244, 835
Bse1I ACTGG 2 cut(s) 16, 81
Bse3DI GCAATG 1 cut(s) 405
BseCI ATCGAT 1 cut(s) 970
BseDI CCNNGG 1 cut(s) 449
BseGI GGATG 3 cut(s) 439, 646, 756
BseLI CCNNNNNNNGG 7 cut(s) 34, 35, 62, 76, 105, 244, 835
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 2 cut(s) 365, 434
BseNI ACTGG 2 cut(s) 16, 81
BseRI GAGGAG 3 cut(s) 144, 517, 854
BseXI GCAGC 2 cut(s) 729, 786
BsgI GTGCAG 1 cut(s) 368
Bsh1236I CGCG 1 cut(s) 925
BshFI GGCC 3 cut(s) 448, 791, 939
BshVI ATCGAT 1 cut(s) 970
BsiHKAI GWGCWC 2 cut(s) 869, 896
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 700
BslI CCNNNNNNNGG 7 cut(s) 34, 35, 62, 76, 105, 244, 835
BsmAI GTCTC 1 cut(s) 452
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 1 cut(s) 700
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 3 cut(s) 448, 791, 939
Bsp119I TTCGAA 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 869, 896
Bsp143I GATC 1 cut(s) 915
BspACI CCGC 1 cut(s) 836
BspANI GGCC 3 cut(s) 448, 791, 939
BspCNI CTCAG 2 cut(s) 366, 435
BspDI ATCGAT 1 cut(s) 970
BspFNI CGCG 1 cut(s) 925
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 40
BspMAI CTGCAG 2 cut(s) 747, 804
BspT104I TTCGAA 1 cut(s) 720
BspTI CTTAAG 1 cut(s) 944
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 2 cut(s) 16, 81
BssECI CCNNGG 1 cut(s) 449
BssMI GATC 1 cut(s) 915
Bst4CI ACNGT 3 cut(s) 176, 383, 707
Bst6I CTCTTC 1 cut(s) 542
BstAFI CTTAAG 1 cut(s) 944
BstAPI GCANNNNNTGC 1 cut(s) 118
BstBAI YACGTR 1 cut(s) 903
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 4 cut(s) 6, 896, 937, 941
BstDEI CTNAG 5 cut(s) 374, 443, 464, 590, 626
BstDSI CCRYGG 1 cut(s) 449
BstF5I GGATG 3 cut(s) 439, 646, 756
BstFNI CGCG 1 cut(s) 925
BstH2I RGCGCY 1 cut(s) 734
BstHHI GCGC 1 cut(s) 733
BstKTI GATC 1 cut(s) 918
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 1 cut(s) 915
BstMWI GCNNNNNNNGC 3 cut(s) 118, 739, 949
BstNSI RCATGY 1 cut(s) 569
BstSFI CTRYAG 2 cut(s) 743, 800
BstSNI TACGTA 1 cut(s) 903
BstUI CGCG 1 cut(s) 925
BstV1I GCAGC 2 cut(s) 729, 786
Bsu15I ATCGAT 1 cut(s) 970
BsuRI GGCC 3 cut(s) 448, 791, 939
BsuTUI ATCGAT 1 cut(s) 970
BtgI CCRYGG 1 cut(s) 449
BtrI CACGTC 1 cut(s) 430
BtsCI GGATG 3 cut(s) 439, 646, 756
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 74, 193
Cac8I GCNNGC 4 cut(s) 6, 896, 937, 941
CciI TCATGA 1 cut(s) 199
CfoI GCGC 1 cut(s) 733
Cfr13I GGNCC 1 cut(s) 48
ClaI ATCGAT 1 cut(s) 970
CseI GACGC 2 cut(s) 469, 941
Csp6I GTAC 1 cut(s) 826
CviAII CATG 3 cut(s) 200, 566, 881
CviQI GTAC 1 cut(s) 826
DdeI CTNAG 5 cut(s) 374, 443, 464, 590, 626
DpnI GATC 1 cut(s) 917
DpnII GATC 1 cut(s) 915
EaeI YGGCCR 2 cut(s) 789, 937
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
Ecl136II GAGCTC 1 cut(s) 867
Eco105I TACGTA 1 cut(s) 903
Eco24I GRGCYC 1 cut(s) 869
Eco47I GGWCC 1 cut(s) 48
Eco47III AGCGCT 1 cut(s) 732
Eco53kI GAGCTC 1 cut(s) 867
EcoICRI GAGCTC 1 cut(s) 867
EcoO109I RGGNCCY 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 869
Esp3I CGTCTC 1 cut(s) 452
FaeI CATG 3 cut(s) 203, 569, 884
FaqI GGGAC 1 cut(s) 700
FatI CATG 3 cut(s) 199, 565, 880
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 2 cut(s) 743, 800
FokI GGATG 3 cut(s) 446, 653, 763
FriOI GRGCYC 1 cut(s) 869
Fsp4HI GCNGC 2 cut(s) 743, 800
GlaI GCGC 1 cut(s) 732
GluI GCNGC 2 cut(s) 743, 800
GsuI CTGGAG 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 734
HaeIII GGCC 3 cut(s) 448, 791, 939
HapII CCGG 1 cut(s) 395
HgaI GACGC 2 cut(s) 469, 941
HhaI GCGC 1 cut(s) 733
Hin1II CATG 3 cut(s) 203, 569, 884
Hin6I GCGC 1 cut(s) 731
HinP1I GCGC 1 cut(s) 731
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HinfI GANTC 7 cut(s) 10, 66, 262, 571, 595, 619, 631
HpaII CCGG 1 cut(s) 395
HphI GGTGA 4 cut(s) 45, 164, 281, 349
Hpy166II GTNNAC 3 cut(s) 164, 405, 703
Hpy188I TCNGA 3 cut(s) 444, 862, 957
Hpy188III TCNNGA 2 cut(s) 139, 200
Hpy8I GTNNAC 3 cut(s) 164, 405, 703
Hpy99I CGWCG 2 cut(s) 367, 957
HpyAV CCTTC 3 cut(s) 136, 487, 598
HpyCH4III ACNGT 3 cut(s) 176, 383, 707
HpyCH4IV ACGT 2 cut(s) 429, 902
HpyCH4V TGCA 7 cut(s) 349, 390, 565, 745, 802, 874, 967
HpyF10VI GCNNNNNNNGC 3 cut(s) 118, 739, 949
HpyF3I CTNAG 5 cut(s) 374, 443, 464, 590, 626
HpySE526I ACGT 2 cut(s) 429, 902
Hsp92II CATG 3 cut(s) 203, 569, 884
HspAI GCGC 1 cut(s) 731
Kzo9I GATC 1 cut(s) 915
LmnI GCTCC 3 cut(s) 480, 496, 872
Lsp1109I GCAGC 2 cut(s) 729, 786
LweI GCATC 1 cut(s) 962
MaeII ACGT 2 cut(s) 429, 902
MaeIII GTNAC 2 cut(s) 377, 781
MalI GATC 1 cut(s) 917
MboI GATC 1 cut(s) 915
MboII GAAGA 6 cut(s) 226, 244, 352, 556, 559, 622
MhlI GDGCHC 2 cut(s) 869, 896
MlsI TGGCCA 2 cut(s) 791, 939
MluCI AATT 2 cut(s) 308, 486
MluI ACGCGT 1 cut(s) 923
MluNI TGGCCA 2 cut(s) 791, 939
MlyI GAGTC 3 cut(s) 60, 628, 640
MmeI TCCRAC 3 cut(s) 393, 840, 935
Mox20I TGGCCA 2 cut(s) 791, 939
MscI TGGCCA 2 cut(s) 791, 939
MseI TTAA 3 cut(s) 540, 672, 945
MslI CAYNNNNRTG 2 cut(s) 177, 879
Msp20I TGGCCA 2 cut(s) 791, 939
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 1 cut(s) 395
Mva1269I GAATGC 1 cut(s) 390
MvnI CGCG 1 cut(s) 925
MwoI GCNNNNNNNGC 3 cut(s) 118, 739, 949
NdeII GATC 1 cut(s) 915
NlaIII CATG 3 cut(s) 203, 569, 884
NlaIV GGNNCC 1 cut(s) 40
NspI RCATGY 1 cut(s) 569
NspV TTCGAA 1 cut(s) 720
PagI TCATGA 1 cut(s) 199
PctI GAATGC 1 cut(s) 390
PfeI GAWTC 4 cut(s) 10, 262, 571, 595
PkrI GCNGC 2 cut(s) 744, 801
PleI GAGTC 3 cut(s) 60, 627, 639
PpsI GAGTC 3 cut(s) 60, 627, 639
Ppu21I YACGTR 1 cut(s) 903
PpuMI RGGWCCY 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 869
Psp5II RGGWCCY 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 1 cut(s) 48
PspPPI RGGWCCY 1 cut(s) 48
PstI CTGCAG 2 cut(s) 747, 804
RsaI GTAC 1 cut(s) 827
RsaNI GTAC 1 cut(s) 826
RseI CAYNNNNRTG 2 cut(s) 177, 879
SacI GAGCTC 1 cut(s) 869
SaqAI TTAA 3 cut(s) 540, 672, 945
SatI GCNGC 2 cut(s) 743, 800
Sau3AI GATC 1 cut(s) 915
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 3 cut(s) 60, 628, 640
SduI GDGCHC 2 cut(s) 869, 896
SetI ASST 9 cut(s) 53, 432, 554, 715, 775, 783, 869, 905, 945
SfaNI GCATC 1 cut(s) 962
SfcI CTRYAG 2 cut(s) 743, 800
SfuI TTCGAA 1 cut(s) 720
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 177, 879
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
SnaBI TACGTA 1 cut(s) 903
Sse9I AATT 2 cut(s) 308, 486
SsiI CCGC 1 cut(s) 836
SstI GAGCTC 1 cut(s) 869
TaaI ACNGT 3 cut(s) 176, 383, 707
TaiI ACGT 2 cut(s) 432, 905
TaqI TCGA 6 cut(s) 87, 362, 531, 720, 918, 970
TasI AATT 2 cut(s) 308, 486
TfiI GAWTC 4 cut(s) 10, 262, 571, 595
Tru1I TTAA 3 cut(s) 540, 672, 945
Tru9I TTAA 3 cut(s) 540, 672, 945
TscAI CASTG 2 cut(s) 81, 193
TseI GCWGC 2 cut(s) 742, 799
TspDTI ATGAA 3 cut(s) 188, 216, 662
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 81, 193
Vha464I CTTAAG 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 48
XceI RCATGY 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.