Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0006344

Physical Location & Seq

Genomic Coordinates Reverse (-)

2739704 .. 2742225

Gene Structure 2522 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00335330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGCTTACGAAGCTCCAGATGGACTTCCCCATTTTGGGTTCCACAAGGACCTCACCCAACACAGGACTCCCTCCACTGGAGAAACTCGAACTCATAACCAGAGATAGGAAGCAGAGAGTGCTTCACGGAGGAGGGTTCAAGAAGGGTTTTAGTGAAGTTTGTAGACCCAGACATAGTAATGGTGGCAGTGTTGTTGTTCATGAAGTTGGGTTGAAGAAGCGTGGTTTTGTGAAGAACCCAGATGAGGAAAAGGGATTAGAGACTCTGGGTGATGGTGGGTTTGTGGAAAGAAAGGATTTTGGGGTTGAATTGGGGTTGGGGGGTGGGAATGGTGTGGTGAAGAAAGTGCAGACCAAGTGTTCGACGAAATGGCTGAGTTACGGTGGGTGCATTCCGGCAATGTTGACGGCGTTGGAGGAAGTTGAGGACTTGGATGAGGCTCTGAGGCCGTGGGGAGAGACGCTTAGTAACAAGGAAAGGAGCATAATTTTGAAGGAGCAGAGGAGTTGGGAAAGGGCTTTGGAGATTTTCGAGTGGTTTAAGAAGAAGGGATGTTATGAGTTGCATGTGATTCACTATAATATAGTGCTTAGGATTCTTGGGAAGGCGAAGAAGTGGAGTCATCTAAGGAGTCTTTGGGATGAGATGAATGTGGAAAGAATAGCACCCATTAACTCAACCTATGGAACTTTGATTGATGTTTATAGTAAAGGTGGGTTCGAAAAGGAAGCGCTTATTTGGCTGCAGAGGATGACCAAGCAAGGAATGAAACCTGATGAGGTTACTATGGCCATTGTGTTGCAGTTGTATAAGAAAGCAGGGGAGTACCGAAAAGCTGAGGAGTTTTTCGAGAAATGGTCAGAGAGCTCGTTGCATTGTCATGGCTCTTTGAGCTCACATACGTATAACACATTGATCGACACGCATGGAAAGGCTGGCCAGCTTAAAGAAGCATCGGAAATATTTGCATCGATGCTGAGGGAAGGGATTGCTCCAACTACAGTGACTTTCAATACAATGATTCACATTTGCGGTAATCATGGTCAGCTTGCTGAAGTTGCTTCGCTTATGCAGAAGATGGAAGAGGTTCGATGCCCACCAGACACGAGAACATATAATATTCTGATTTCCCTCCATGCTAGGAATGATAATATAGACACAGCTACAAACTACTTTGCAAAGATGAAGGAGGCTTGCCTTGAGCCAGACCCTGTGAGTTATCGCACCCTTTTGTATGCATACTCTGTACGGCACATGGTTAGCAAAGCTGAAGAGCTCATATCAGAGATGGATGAAAGGGGCTTGAGATTGATGAGGAAAATGGGATCTGATTGCTATTCTGCCAACATTGATGCATATGGAGAGCGTGGGCATATTTTGGAAGCTGAGAAAGTTTTCAATTGCTGCCGAGAAGTGAAGAAGTTGAGTGTCCTTGAGTTCAACGTGATGATCAAAGCATATGGGGTTGGTAAACATTACAGTAGAGCTTGCCAGTTGTTTGATAGCATGGAGAATCATGGTGTAATTCCGGACAGGTGTAGCTATAGTTCTCTGATACAAATTTTGGCTAGTGGTGATATGCCACATACTGCAATGCCTTATCTGAAGAAGATGCAGGAGTCAGGGTTGGTACGTGATTGCATCGCCTATTGTGCTGTGATATCAAGCTTTGCAAAATTAGGTCAATTGGAAAGGGCAGAGGACGTTTATAAACAGATGGTTGGATTCAATGTGCAGCCTGATGTAATTGTCTTTGGGGTGTTGATAAATGCTTTTGCTGAAGTTGGAAGCGTTAAAAAAGCTCTCGGTTATGCTGATGCAATGAAAAGGGCAGGTTTCCCTGGGAACACAGTTGTTTACAATACCTTGATCAAGCTCTATACAAAAGTTGGCCTTTTGAAAGAAGCCGAAGAAACATACAAACTTCTTCAGTCATCGGAGGATGGTCCTGCTATATATGCCTCAAATTGTGTGATTGATCTTTATAGCGAGCGATGTATGGTTAAACCAGCAGAAGAACTCTTTGACAGCTTGAAGAGAAAGGGGGATGCAAATGAGTTTACATTTGCAATGATGTTGTGCATGTATAAGAGAATTGGGAGGTTTGAGGAAGCCCTTCAGATTGCAAAGCAGATGAGAGAGCTGCGACTTTTAACTGATCTGCTGAGTTATAATAATGTTATTGGGTTGTATGCGACATATGGAAGATTCAAAGAGGTGGTGGAAACTTTCAAGGAAATGACAAAAGCTGCCATTCAACCTGATGAATGCACATTCAAATCACTGGGACTTGTTTTGGTGAAATCTGGACTTTCAAAGCAGGCTGTGGGAAAACTGGAAGTATCAGTTAAGAGGGATGCTCGGAGTGGTTTGCAAGCTTGGATGTCAGCCCTCTCTGCTGTTGTCAGAGTGAATGAAAGTAGTTATATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

810

Amino Acids

91.21

Weight (kDa)

8.47

Isoelectric Point (pI)

39.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	295 - 394	2.8e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	296 - 412	1.1e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	299 - 344	1e-12	PPR repeat family
PPR_3	PF13812	300 - 346	1.3e-09	Pentatricopeptide repeat domain
PPR	PF01535	301 - 330	2e-06	PPR repeat
PPR_2	PF13041	332 - 378	4.7e-12	PPR repeat family
PPR_2	PF13041	367 - 414	1.4e-08	PPR repeat family
TPR_24	PF23276	472 - 573	4e-10	Fungal tetratrico peptide repeats
PPR_3	PF13812	474 - 521	1e-05	Pentatricopeptide repeat domain
PPR_2	PF13041	480 - 523	9.6e-08	PPR repeat family
PPR_long	PF17177	492 - 645	1.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	546 - 593	2.4e-07	PPR repeat family
PPR_2	PF13041	580 - 625	9.5e-06	PPR repeat family
PPR_2	PF13041	721 - 762	9.7e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23020
fragaria_vesca	FvH4_4g02020
malus_domestica	MD13G1203500.v1.1 MD16G1205100.v1.1
prunus_persica	Prupe.1G026500_v2.0.a1
pyrus_communis	pycom13g17680
rosa_chinensis	RchiOBHm_Chr4g0388991 RchiOBHm_Chr4g0389031 RchiOBHm_Chr4g0389081 RchiOBHm_Chr4g0389161 RchiOBHm_Chr4g0389171
rosa_laevigata	RLG00000010001
rosa_multiflora	Rmu_sc0001395.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335330
rosa_rugosa	Rorug03G0321900
rosa_samantha	Rh4AG026400 Rh4BG019600 Rh4BG019800 Rh4BG019900 Rh4BG020200 Rh4BG020300 Rh4CG029400 Rh4DG020000 Rh4DG020400 Rh4DG020500 Rh4DG020900
rosa_wichuraiana	Rw4G001950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1710, 2172
Acc36I	ACCTGC	1 cut(s)	1823
AccI	GTMKAC	1 cut(s)	163
AccIII	TCCGGA	1 cut(s)	1528
AciI	CCGC	1 cut(s)	1032
AclWI	GGATC	1 cut(s)	1333
AcoI	YGGCCR	2 cut(s)	789, 937
AcsI	RAATTY	1 cut(s)	1560
AcuI	CTGAAG	6 cut(s)	1074, 1290, 1625, 1800, 1913, 2102
AfaI	GTAC	3 cut(s)	827, 1248, 1632
AfeI	AGCGCT	1 cut(s)	732
AfiI	CCNNNNNNNGG	6 cut(s)	34, 35, 62, 76, 105, 244
AjnI	CCWGG	1 cut(s)	1840
Alw21I	GWGCWC	3 cut(s)	869, 896, 1278
Alw26I	GTCTC	2 cut(s)	254, 452
AlwI	GGATC	1 cut(s)	1333
AlwNI	CAGNNNCTG	1 cut(s)	1211
Aor13HI	TCCGGA	1 cut(s)	1528
Aor51HI	AGCGCT	1 cut(s)	732
AoxI	GGCC	4 cut(s)	446, 789, 937, 1891
ApeKI	GCWGC	5 cut(s)	742, 1404, 1735, 2143, 2249
ApoI	RAATTY	1 cut(s)	1560
ArsI	GACNNNNNNTTYG	2 cut(s)	343, 375
Asp700I	GAANNNNTTC	3 cut(s)	1086, 1394, 2115
AspLEI	GCGC	1 cut(s)	733
AspS9I	GGNCC	2 cut(s)	48, 1946
AsuHPI	GGTGA	5 cut(s)	45, 281, 349, 1586, 2311
AsuII	TTCGAA	1 cut(s)	720
AvaII	GGWCC	2 cut(s)	48, 1946
BalI	TGGCCA	2 cut(s)	791, 939
BanII	GRGCYC	3 cut(s)	869, 896, 1278
BauI	CACGAG	1 cut(s)	1105
Bbv12I	GWGCWC	3 cut(s)	869, 896, 1278
BbvCI	CCTCAGC	2 cut(s)	837, 977
BbvI	GCAGC	5 cut(s)	729, 1391, 1747, 2130, 2236
BccI	CCATC	6 cut(s)	13, 266, 1072, 1282, 1711, 1937
BceAI	ACGGC	3 cut(s)	423, 433, 1265
BciT130I	CCWGG	1 cut(s)	1842
BclI	TGATCA	2 cut(s)	1449, 1869
BcoDI	GTCTC	2 cut(s)	254, 452
BfaI	CTAG	2 cut(s)	1140, 1569
BfmI	CTRYAG	3 cut(s)	743, 999, 1543
BfoI	RGCGCY	1 cut(s)	734
BfuAI	ACCTGC	1 cut(s)	1823
BisI	GCNGC	5 cut(s)	743, 1405, 1736, 2144, 2250
BlsI	GCNGC	5 cut(s)	744, 1406, 1737, 2145, 2251
Bme1390I	CCNGG	1 cut(s)	1842
Bme18I	GGWCC	2 cut(s)	48, 1946
BmgT120I	GGNCC	2 cut(s)	48, 1946
BmiI	GGNNCC	1 cut(s)	40
BmrFI	CCNGG	1 cut(s)	1842
BmrI	ACTGGG	1 cut(s)	2294
BmuI	ACTGGG	1 cut(s)	2294
BplI	GAGNNNNNCTC	2 cut(s)	2344, 2376
BpmI	CTGGAG	1 cut(s)	98
Bpu10I	CCTNAGC	3 cut(s)	590, 837, 977
Bpu14I	TTCGAA	1 cut(s)	720
BpuEI	CTTGAG	3 cut(s)	1220, 1324, 1454
Bsa29I	ATCGAT	1 cut(s)	971
BsaAI	YACGTR	2 cut(s)	903, 1634
BsaJI	CCNNGG	3 cut(s)	449, 1840, 1841
BsaWI	WCCGGW	1 cut(s)	1528
BsaXI	ACNNNNNCTCC	2 cut(s)	1610, 1640
Bsc4I	CCNNNNNNNGG	6 cut(s)	34, 35, 62, 76, 105, 244
Bse1I	ACTGG	4 cut(s)	81, 1492, 2289, 2340
Bse3DI	GCAATG	4 cut(s)	405, 1599, 1827, 2076
BseAI	TCCGGA	1 cut(s)	1528
BseBI	CCWGG	1 cut(s)	1842
BseCI	ATCGAT	1 cut(s)	971
BseDI	CCNNGG	3 cut(s)	449, 1840, 1841
BseGI	GGATG	9 cut(s)	439, 557, 646, 756, 1297, 1948, 2053, 2362, 2388
BseLI	CCNNNNNNNGG	6 cut(s)	34, 35, 62, 76, 105, 244
BseMI	GCAATG	4 cut(s)	405, 1599, 1827, 2076
BseMII	CTCAG	6 cut(s)	365, 434, 828, 968, 1377, 2156
BseNI	ACTGG	4 cut(s)	81, 1492, 2289, 2340
BseRI	GAGGAG	3 cut(s)	144, 517, 854
BseXI	GCAGC	5 cut(s)	729, 1391, 1747, 2130, 2236
BsgI	GTGCAG	2 cut(s)	368, 1754
BshFI	GGCC	4 cut(s)	448, 791, 939, 1893
BshVI	ATCGAT	1 cut(s)	971
BsiHKAI	GWGCWC	3 cut(s)	869, 896, 1278
BsiSI	CCGG	2 cut(s)	395, 1529
BslFI	GGGAC	1 cut(s)	2301
BslI	CCNNNNNNNGG	6 cut(s)	34, 35, 62, 76, 105, 244
BsmAI	GTCTC	2 cut(s)	254, 452
BsmBI	CGTCTC	1 cut(s)	452
BsmFI	GGGAC	1 cut(s)	2301
BsmI	GAATGC	2 cut(s)	390, 2273
BsnI	GGCC	4 cut(s)	448, 791, 939, 1893
Bsp119I	TTCGAA	1 cut(s)	720
Bsp1286I	GDGCHC	3 cut(s)	869, 896, 1278
Bsp13I	TCCGGA	1 cut(s)	1528
Bsp143I	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
BspACI	CCGC	1 cut(s)	1032
BspANI	GGCC	4 cut(s)	448, 791, 939, 1893
BspCNI	CTCAG	6 cut(s)	366, 435, 829, 969, 1378, 2157
BspDI	ATCGAT	1 cut(s)	971
BspEI	TCCGGA	1 cut(s)	1528
BspHI	TCATGA	1 cut(s)	199
BspLI	GGNNCC	1 cut(s)	40
BspMAI	CTGCAG	1 cut(s)	747
BspMI	ACCTGC	1 cut(s)	1823
BspPI	GGATC	1 cut(s)	1333
BspQI	GCTCTTC	1 cut(s)	1266
BspT104I	TTCGAA	1 cut(s)	720
BsrDI	GCAATG	4 cut(s)	405, 1599, 1827, 2076
BsrI	ACTGG	4 cut(s)	81, 1492, 2289, 2340
BssECI	CCNNGG	3 cut(s)	449, 1840, 1841
BssMI	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
BssSI	CACGAG	1 cut(s)	1105
Bst2BI	CACGAG	1 cut(s)	1105
Bst2UI	CCWGG	1 cut(s)	1842
Bst4CI	ACNGT	4 cut(s)	383, 1003, 1481, 1852
Bst6I	CTCTTC	3 cut(s)	1077, 1266, 2030
BstAPI	GCANNNNNTGC	1 cut(s)	118
BstBAI	YACGTR	2 cut(s)	903, 1634
BstBI	TTCGAA	1 cut(s)	720
BstC8I	GCNNGC	8 cut(s)	937, 941, 1050, 1195, 1489, 1991, 2322, 2376
BstDEI	CTNAG	9 cut(s)	374, 443, 464, 590, 626, 837, 977, 1386, 2165
BstDSI	CCRYGG	1 cut(s)	449
BstF5I	GGATG	9 cut(s)	439, 557, 646, 756, 1297, 1948, 2053, 2362, 2388
BstH2I	RGCGCY	1 cut(s)	734
BstHHI	GCGC	1 cut(s)	733
BstKTI	GATC	6 cut(s)	918, 1328, 1452, 1872, 1981, 2161
BstMAI	GTCTC	2 cut(s)	254, 452
BstMBI	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
BstMWI	GCNNNNNNNGC	8 cut(s)	10, 118, 739, 891, 1058, 1652, 1958, 2396
BstNI	CCWGG	1 cut(s)	1842
BstNSI	RCATGY	2 cut(s)	569, 2086
BstSCI	CCNGG	1 cut(s)	1840
BstSFI	CTRYAG	3 cut(s)	743, 999, 1543
BstSNI	TACGTA	1 cut(s)	903
BstV1I	GCAGC	5 cut(s)	729, 1391, 1747, 2130, 2236
BstX2I	RGATCY	1 cut(s)	1325
BstYI	RGATCY	1 cut(s)	1325
Bsu15I	ATCGAT	1 cut(s)	971
BsuRI	GGCC	4 cut(s)	448, 791, 939, 1893
BsuTUI	ATCGAT	1 cut(s)	971
BtgI	CCRYGG	1 cut(s)	449
BtgZI	GCGATG	2 cut(s)	1627, 2008
BtsCI	GGATG	9 cut(s)	439, 557, 646, 756, 1297, 1948, 2053, 2362, 2388
BtsI	GCAGTG	1 cut(s)	193
BtsIMutI	CAGTG	4 cut(s)	74, 193, 1008, 2282
BveI	ACCTGC	1 cut(s)	1823
Cac8I	GCNNGC	8 cut(s)	937, 941, 1050, 1195, 1489, 1991, 2322, 2376
CaiI	CAGNNNCTG	1 cut(s)	1211
CciI	TCATGA	1 cut(s)	199
CfoI	GCGC	1 cut(s)	733
Cfr13I	GGNCC	2 cut(s)	48, 1946
ClaI	ATCGAT	1 cut(s)	971
CseI	GACGC	1 cut(s)	469
Csp6I	GTAC	3 cut(s)	826, 1247, 1631
CviQI	GTAC	3 cut(s)	826, 1247, 1631
DdeI	CTNAG	9 cut(s)	374, 443, 464, 590, 626, 837, 977, 1386, 2165
DpnI	GATC	6 cut(s)	917, 1327, 1451, 1871, 1980, 2160
DpnII	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
EaeI	YGGCCR	2 cut(s)	789, 937
Eam1104I	CTCTTC	3 cut(s)	1077, 1266, 2030
EarI	CTCTTC	3 cut(s)	1077, 1266, 2030
Ecl136II	GAGCTC	3 cut(s)	867, 894, 1276
Eco105I	TACGTA	1 cut(s)	903
Eco24I	GRGCYC	3 cut(s)	869, 896, 1278
Eco32I	GATATC	1 cut(s)	1662
Eco47I	GGWCC	2 cut(s)	48, 1946
Eco47III	AGCGCT	1 cut(s)	732
Eco53kI	GAGCTC	3 cut(s)	867, 894, 1276
Eco57I	CTGAAG	6 cut(s)	1074, 1290, 1625, 1800, 1913, 2102
EcoICRI	GAGCTC	3 cut(s)	867, 894, 1276
EcoO109I	RGGNCCY	1 cut(s)	48
EcoRII	CCWGG	1 cut(s)	1840
EcoRV	GATATC	1 cut(s)	1662
EcoT22I	ATGCAT	2 cut(s)	1240, 1357
EcoT38I	GRGCYC	3 cut(s)	869, 896, 1278
Esp3I	CGTCTC	1 cut(s)	452
FalI	AAGNNNNNCTT	2 cut(s)	1878, 1910
FaqI	GGGAC	1 cut(s)	2301
FauNDI	CATATG	3 cut(s)	1357, 1459, 2200
FbaI	TGATCA	2 cut(s)	1449, 1869
FblI	GTMKAC	1 cut(s)	163
Fnu4HI	GCNGC	5 cut(s)	743, 1405, 1736, 2144, 2250
FokI	GGATG	9 cut(s)	446, 564, 653, 763, 1304, 1955, 2060, 2369, 2395
FriOI	GRGCYC	3 cut(s)	869, 896, 1278
Fsp4HI	GCNGC	5 cut(s)	743, 1405, 1736, 2144, 2250
FspBI	CTAG	2 cut(s)	1140, 1569
GlaI	GCGC	1 cut(s)	732
GluI	GCNGC	5 cut(s)	743, 1405, 1736, 2144, 2250
GsuI	CTGGAG	1 cut(s)	98
HaeII	RGCGCY	1 cut(s)	734
HaeIII	GGCC	4 cut(s)	448, 791, 939, 1893
HapII	CCGG	2 cut(s)	395, 1529
HgaI	GACGC	1 cut(s)	469
HhaI	GCGC	1 cut(s)	733
Hin6I	GCGC	1 cut(s)	731
HinP1I	GCGC	1 cut(s)	731
HincII	GTYRAC	1 cut(s)	405
HindII	GTYRAC	1 cut(s)	405
HindIII	AAGCTT	2 cut(s)	1666, 2376
HpaII	CCGG	2 cut(s)	395, 1529
HphI	GGTGA	5 cut(s)	45, 281, 349, 1586, 2311
Hpy166II	GTNNAC	5 cut(s)	164, 405, 1472, 1858, 2061
Hpy188III	TCNNGA	6 cut(s)	16, 139, 200, 850, 1529, 2307
Hpy8I	GTNNAC	5 cut(s)	164, 405, 1472, 1858, 2061
Hpy99I	CGWCG	1 cut(s)	367
HpyAV	CCTTC	7 cut(s)	136, 487, 541, 598, 977, 1180, 2126
HpyCH4III	ACNGT	4 cut(s)	383, 1003, 1481, 1852
HpyCH4IV	ACGT	4 cut(s)	902, 1443, 1633, 1704
HpyF10VI	GCNNNNNNNGC	8 cut(s)	10, 118, 739, 891, 1058, 1652, 1958, 2396
HpyF3I	CTNAG	9 cut(s)	374, 443, 464, 590, 626, 837, 977, 1386, 2165
HpySE526I	ACGT	4 cut(s)	902, 1443, 1633, 1704
HspAI	GCGC	1 cut(s)	731
Kpn2I	TCCGGA	1 cut(s)	1528
Ksp22I	TGATCA	2 cut(s)	1449, 1869
Kzo9I	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
LguI	GCTCTTC	1 cut(s)	1266
LmnI	GCTCC	4 cut(s)	18, 480, 496, 997
Lsp1109I	GCAGC	5 cut(s)	729, 1391, 1747, 2130, 2236
MaeI	CTAG	2 cut(s)	1140, 1569
MaeII	ACGT	4 cut(s)	902, 1443, 1633, 1704
MaeIII	GTNAC	4 cut(s)	377, 467, 781, 1003
MalI	GATC	6 cut(s)	917, 1327, 1451, 1871, 1980, 2160
MboI	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
MfeI	CAATTG	2 cut(s)	1399, 1685
MflI	RGATCY	1 cut(s)	1325
MhlI	GDGCHC	3 cut(s)	869, 896, 1278
MlsI	TGGCCA	2 cut(s)	791, 939
MluNI	TGGCCA	2 cut(s)	791, 939
MlyI	GAGTC	5 cut(s)	60, 256, 628, 640, 1628
MmeI	TCCRAC	4 cut(s)	393, 1019, 1702, 1765
Mox20I	TGGCCA	2 cut(s)	791, 939
Mph1103I	ATGCAT	2 cut(s)	1240, 1357
MroI	TCCGGA	1 cut(s)	1528
MroXI	GAANNNNTTC	3 cut(s)	1086, 1394, 2115
MscI	TGGCCA	2 cut(s)	791, 939
MseI	TTAA	7 cut(s)	540, 672, 945, 1794, 2004, 2153, 2349
MslI	CAYNNNNRTG	2 cut(s)	177, 879
Msp20I	TGGCCA	2 cut(s)	791, 939
MspI	CCGG	2 cut(s)	395, 1529
MspR9I	CCNGG	1 cut(s)	1842
MunI	CAATTG	2 cut(s)	1399, 1685
Mva1269I	GAATGC	2 cut(s)	390, 2273
MvaI	CCWGG	1 cut(s)	1842
MwoI	GCNNNNNNNGC	8 cut(s)	10, 118, 739, 891, 1058, 1652, 1958, 2396
NdeI	CATATG	3 cut(s)	1357, 1459, 2200
NdeII	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
NlaIV	GGNNCC	1 cut(s)	40
NmeAIII	GCCGAG	1 cut(s)	1433
NmuCI	GTSAC	1 cut(s)	1003
NsiI	ATGCAT	2 cut(s)	1240, 1357
NspI	RCATGY	2 cut(s)	569, 2086
NspV	TTCGAA	1 cut(s)	720
PagI	TCATGA	1 cut(s)	199
PasI	CCCWGGG	1 cut(s)	1841
PciSI	GCTCTTC	1 cut(s)	1266
PctI	GAATGC	2 cut(s)	390, 2273
PdmI	GAANNNNTTC	3 cut(s)	1086, 1394, 2115
PfeI	GAWTC	6 cut(s)	571, 595, 1021, 1513, 1725, 2208
PkrI	GCNGC	5 cut(s)	744, 1406, 1737, 2145, 2251
PleI	GAGTC	5 cut(s)	60, 256, 627, 639, 1627
PpsI	GAGTC	5 cut(s)	60, 256, 627, 639, 1627
Ppu21I	YACGTR	2 cut(s)	903, 1634
PpuMI	RGGWCCY	1 cut(s)	48
PsiI	TTATAA	2 cut(s)	1710, 2172
Psp124BI	GAGCTC	3 cut(s)	869, 896, 1278
Psp5II	RGGWCCY	1 cut(s)	48
Psp6I	CCWGG	1 cut(s)	1840
PspGI	CCWGG	1 cut(s)	1840
PspN4I	GGNNCC	1 cut(s)	40
PspPI	GGNCC	2 cut(s)	48, 1946
PspPPI	RGGWCCY	1 cut(s)	48
PsrI	GAACNNNNNNTAC	2 cut(s)	1531, 1563
PstI	CTGCAG	1 cut(s)	747
PstNI	CAGNNNCTG	1 cut(s)	1211
PsuI	RGATCY	1 cut(s)	1325
RsaI	GTAC	3 cut(s)	827, 1248, 1632
RsaNI	GTAC	3 cut(s)	826, 1247, 1631
RseI	CAYNNNNRTG	2 cut(s)	177, 879
SacI	GAGCTC	3 cut(s)	869, 896, 1278
SapI	GCTCTTC	1 cut(s)	1266
SaqAI	TTAA	7 cut(s)	540, 672, 945, 1794, 2004, 2153, 2349
SatI	GCNGC	5 cut(s)	743, 1405, 1736, 2144, 2250
Sau3AI	GATC	6 cut(s)	915, 1325, 1449, 1869, 1978, 2158
Sau96I	GGNCC	2 cut(s)	48, 1946
SchI	GAGTC	5 cut(s)	60, 256, 628, 640, 1628
ScrFI	CCNGG	1 cut(s)	1842
SduI	GDGCHC	3 cut(s)	869, 896, 1278
SfcI	CTRYAG	3 cut(s)	743, 999, 1543
SfuI	TTCGAA	1 cut(s)	720
SinI	GGWCC	2 cut(s)	48, 1946
SmiMI	CAYNNNNRTG	2 cut(s)	177, 879
SmlI	CTYRAG	3 cut(s)	1199, 1303, 1433
SmoI	CTYRAG	3 cut(s)	1199, 1303, 1433
SnaBI	TACGTA	1 cut(s)	903
SsiI	CCGC	1 cut(s)	1032
SspI	AATATT	2 cut(s)	963, 1120
SspMI	CTAG	2 cut(s)	1140, 1569
SstI	GAGCTC	3 cut(s)	869, 896, 1278
StyD4I	CCNGG	1 cut(s)	1840
TaaI	ACNGT	4 cut(s)	383, 1003, 1481, 1852
TaiI	ACGT	4 cut(s)	905, 1446, 1636, 1707
TaqI	TCGA	8 cut(s)	87, 362, 531, 720, 849, 918, 971, 1090
TfiI	GAWTC	6 cut(s)	571, 595, 1021, 1513, 1725, 2208
Tru1I	TTAA	7 cut(s)	540, 672, 945, 1794, 2004, 2153, 2349
Tru9I	TTAA	7 cut(s)	540, 672, 945, 1794, 2004, 2153, 2349
TscAI	CASTG	4 cut(s)	81, 193, 1008, 2289
TseFI	GTSAC	1 cut(s)	1003
TseI	GCWGC	5 cut(s)	742, 1404, 1735, 2143, 2249
Tsp45I	GTSAC	1 cut(s)	1003
TspDTI	ATGAA	9 cut(s)	188, 216, 662, 782, 1199, 1308, 1838, 2280, 2430
TspGWI	ACGGA	1 cut(s)	141
TspRI	CASTG	4 cut(s)	81, 193, 1008, 2289
VpaK11BI	GGWCC	2 cut(s)	48, 1946
XapI	RAATTY	1 cut(s)	1560
XceI	RCATGY	2 cut(s)	569, 2086
XmiI	GTMKAC	1 cut(s)	163
XmnI	GAANNNNTTC	3 cut(s)	1086, 1394, 2115
XspI	CTAG	2 cut(s)	1140, 1569
Zsp2I	ATGCAT	2 cut(s)	1240, 1357

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.