Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0006344

Physical Location & Seq

Genomic Coordinates Reverse (-)

4191747 .. 4193177

Gene Structure 1431 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ36220

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGTGGTTTAAGAAGAAGAGGTGTCATGAGTTGCATGTGATTCACTATAATATAGTGCTTAGGATTCTTGGGAAGGCGAAGAAGTGGAGTCATCTAAGGAGTCTTTGGGATGAGATGAATGTGGAAAGAATGGCACCCATTAACTCAACTTATGGAACTTTGATTGATGTTTACAGTAAAGGTGGGTTCGAAAAGGAAGCGCTCATTTGGCTGCAGAGGATGACCAAGCAAGGAATGAAACCTGATGAGGTTACTATGGCCATTGTGCTGCAGTTGTATAAGAAAGCAGGGGAGTACCGAAAAGCTGAGGAGTTTTTTGAGAAATGGTCGGAGAGCTCCTTGCATTCTCATGGTTCTTTGAGCTTGCATACGTATAACACATTGATCGACACGCGTGGAAAAGCTGGCCAGCTTAAAGACGCGTCGGAAATGTTTGCATCGATGCTGAGGGAAGGGATTGCTCCAACTACAGTGACTTTCAATACAATGATTCACATTTGCGGTAATCATGGTCAGCTTGCAGAAGTTGCTTCACTTATGCAGAAGATGGAAGAGGTTCGATGCCCACCAGACACGAGAACATATAATATTCTGATTTCCCTCCATGCTAGGCATGATAATATAGACACAGCTACAAACTACTTTGCAAAGATGAAGGAGGCTTGCCTTGAGCCAGACCCTGTGAGTTATCGCACCCTTTTGTATGCATACTCTGTAAGGCACATGGTTAGCAAAGCTGAAGAGCTCATATCAGAGATGGATGAAAGGGGGCTTGAAATTGATGAGTTCACTCATTCGGCTCTGACTAGGATGTATATAGAAGCGGGGATGCTTGAGAAGTCTTGGGTTTGGTTCTTGAGATTTCATCTCTCAGGGAAAATGGGATCTGATTGCTATTCTGCCAACATTGATGCATATGGAGAGCGTGGGCATATTTTGGAAGCTGAGAAAGTTTTCAATTGCTGCCGAGAAGTGAAGAAGTTGAGTGTCCTTGAGTTCAACGTGATGATCAAATCGTATGTGGTTGGTAAACATTACAGTAGAGCTTGCCAGTTGTTTGGTAGCGCGGAGAGTCATAGTGTGATTCCAGACAGTTGTAGCTATAGTTCTCTGATACAAATTTTGGCTAGTGGTGATATGCCACATACTGCAATGCCTTATCTGAAGAAGATGCAGGAGTCAGGGTTGGTACGTGATTGCATCCCCTATTGTGCTGTGATTTCAAGCTTTGCAAAATTAGGTCAATTGGAAAGGGCAGAGGAAGTTTATAAACAGATGGTTCAATTCAATGTGCAGCCTGATGTAATTGTCTTTGGGGTGTTGATAAATGCTTTTGCTGAAGTTGGAAGAAAGATAACTTTTATTAATAAGAAAAATCAAATTGCGTTGTTTATATGGCAATTATATATCGCCTTTATATGTCCTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

476

Amino Acids

54.57

Weight (kDa)

7.91

Isoelectric Point (pI)

38.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	120 - 169	1.4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	123 - 167	1.2e-11	PPR repeat family
PPR_long	PF17177	129 - 281	6.2e-12	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	155 - 201	2.4e-12	PPR repeat family
PPR_2	PF13041	190 - 237	9.5e-09	PPR repeat family
PPR_2	PF13041	225 - 270	5.3e-07	PPR repeat family
TPR_24	PF23276	326 - 426	8.3e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	388 - 445	4.3e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	403 - 446	1.4e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23020
fragaria_vesca	FvH4_4g02020
malus_domestica	MD13G1203500.v1.1 MD16G1205100.v1.1
prunus_persica	Prupe.1G026500_v2.0.a1
pyrus_communis	pycom13g17680
rosa_chinensis	RchiOBHm_Chr4g0388991 RchiOBHm_Chr4g0389031 RchiOBHm_Chr4g0389081 RchiOBHm_Chr4g0389161 RchiOBHm_Chr4g0389171
rosa_laevigata	RLG00000010001
rosa_multiflora	Rmu_sc0001395.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335330
rosa_rugosa	Rorug03G0321900
rosa_samantha	Rh4AG026400 Rh4BG019600 Rh4BG019800 Rh4BG019900 Rh4BG020200 Rh4BG020300 Rh4CG029400 Rh4DG020000 Rh4DG020400 Rh4DG020500 Rh4DG020900
rosa_wichuraiana	Rw4G001950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1269
AccB1I	GGYRCC	1 cut(s)	133
AccII	CGCG	3 cut(s)	394, 422, 1067
AciI	CCGC	3 cut(s)	501, 824, 1067
AclWI	GGATC	1 cut(s)	892
AcoI	YGGCCR	2 cut(s)	258, 406
AcsI	RAATTY	1 cut(s)	1119
AcuI	CTGAAG	3 cut(s)	759, 1184, 1359
AfaI	GTAC	2 cut(s)	296, 1191
AfeI	AGCGCT	1 cut(s)	201
AflIII	ACRYGT	2 cut(s)	392, 420
AgsI	TTSAA	7 cut(s)	481, 776, 958, 1000, 1224, 1283, 1288
Alw21I	GWGCWC	2 cut(s)	338, 747
AlwI	GGATC	1 cut(s)	892
AlwNI	CAGNNNCTG	1 cut(s)	680
Aor51HI	AGCGCT	1 cut(s)	201
AoxI	GGCC	2 cut(s)	258, 406
ApeKI	GCWGC	4 cut(s)	211, 268, 963, 1294
ApoI	RAATTY	1 cut(s)	1119
AseI	ATTAAT	1 cut(s)	1365
Asp700I	GAANNNNTTC	2 cut(s)	555, 953
AspLEI	GCGC	2 cut(s)	202, 1067
AsuHPI	GGTGA	1 cut(s)	1145
AsuII	TTCGAA	1 cut(s)	189
BalI	TGGCCA	2 cut(s)	260, 408
BanI	GGYRCC	1 cut(s)	133
BanII	GRGCYC	2 cut(s)	338, 747
BauI	CACGAG	1 cut(s)	574
Bbv12I	GWGCWC	2 cut(s)	338, 747
BbvCI	CCTCAGC	2 cut(s)	306, 446
BbvI	GCAGC	4 cut(s)	198, 255, 950, 1306
BccI	CCATC	3 cut(s)	541, 751, 1270
BclI	TGATCA	1 cut(s)	1008
BfaI	CTAG	3 cut(s)	609, 807, 1128
BfmI	CTRYAG	4 cut(s)	212, 269, 468, 1102
BfoI	RGCGCY	1 cut(s)	203
BisI	GCNGC	4 cut(s)	212, 269, 964, 1295
BlsI	GCNGC	4 cut(s)	213, 270, 965, 1296
BmiI	GGNNCC	1 cut(s)	135
BmsI	GCATC	7 cut(s)	432, 446, 551, 819, 901, 1161, 1209
Bpu10I	CCTNAGC	3 cut(s)	59, 306, 446
Bpu14I	TTCGAA	1 cut(s)	189
BpuEI	CTTGAG	4 cut(s)	689, 854, 877, 1013
Bsa29I	ATCGAT	1 cut(s)	440
BsaAI	YACGTR	2 cut(s)	372, 1193
BsaXI	ACNNNNNCTCC	2 cut(s)	1169, 1199
Bse1I	ACTGG	1 cut(s)	1051
Bse3DI	GCAATG	1 cut(s)	1158
BseCI	ATCGAT	1 cut(s)	440
BseGI	GGATG	6 cut(s)	115, 225, 766, 816, 834, 1200
BseMI	GCAATG	1 cut(s)	1158
BseMII	CTCAG	4 cut(s)	297, 437, 885, 936
BseNI	ACTGG	1 cut(s)	1051
BseRI	GAGGAG	1 cut(s)	323
BseXI	GCAGC	4 cut(s)	198, 255, 950, 1306
BsgI	GTGCAG	1 cut(s)	1313
Bsh1236I	CGCG	3 cut(s)	394, 422, 1067
BshFI	GGCC	2 cut(s)	260, 408
BshNI	GGYRCC	1 cut(s)	133
BshVI	ATCGAT	1 cut(s)	440
BsiHKAI	GWGCWC	2 cut(s)	338, 747
BsmI	GAATGC	1 cut(s)	343
BsnI	GGCC	2 cut(s)	260, 408
Bsp119I	TTCGAA	1 cut(s)	189
Bsp1286I	GDGCHC	2 cut(s)	338, 747
Bsp143I	GATC	3 cut(s)	384, 884, 1008
BspACI	CCGC	3 cut(s)	501, 824, 1067
BspANI	GGCC	2 cut(s)	260, 408
BspCNI	CTCAG	4 cut(s)	298, 438, 884, 937
BspDI	ATCGAT	1 cut(s)	440
BspFNI	CGCG	3 cut(s)	394, 422, 1067
BspHI	TCATGA	1 cut(s)	25
BspLI	GGNNCC	1 cut(s)	135
BspMAI	CTGCAG	2 cut(s)	216, 273
BspPI	GGATC	1 cut(s)	892
BspQI	GCTCTTC	1 cut(s)	735
BspT104I	TTCGAA	1 cut(s)	189
BspT107I	GGYRCC	1 cut(s)	133
BsrDI	GCAATG	1 cut(s)	1158
BsrI	ACTGG	1 cut(s)	1051
BssMI	GATC	3 cut(s)	384, 884, 1008
BssSI	CACGAG	1 cut(s)	574
Bst2BI	CACGAG	1 cut(s)	574
Bst4CI	ACNGT	4 cut(s)	176, 472, 1040, 1094
Bst6I	CTCTTC	3 cut(s)	11, 546, 735
BstAPI	GCANNNNNTGC	1 cut(s)	527
BstBAI	YACGTR	2 cut(s)	372, 1193
BstBI	TTCGAA	1 cut(s)	189
BstC8I	GCNNGC	6 cut(s)	365, 406, 410, 519, 664, 1048
BstDEI	CTNAG	6 cut(s)	59, 95, 306, 446, 871, 945
BstF5I	GGATG	6 cut(s)	115, 225, 766, 816, 834, 1200
BstFNI	CGCG	3 cut(s)	394, 422, 1067
BstH2I	RGCGCY	1 cut(s)	203
BstHHI	GCGC	2 cut(s)	202, 1067
BstKTI	GATC	3 cut(s)	387, 887, 1011
BstMBI	GATC	3 cut(s)	384, 884, 1008
BstMWI	GCNNNNNNNGC	2 cut(s)	208, 527
BstNSI	RCATGY	1 cut(s)	38
BstSFI	CTRYAG	4 cut(s)	212, 269, 468, 1102
BstSNI	TACGTA	1 cut(s)	372
BstUI	CGCG	3 cut(s)	394, 422, 1067
BstV1I	GCAGC	4 cut(s)	198, 255, 950, 1306
BstX2I	RGATCY	1 cut(s)	884
BstXI	CCANNNNNNTGG	1 cut(s)	1058
BstYI	RGATCY	1 cut(s)	884
Bsu15I	ATCGAT	1 cut(s)	440
BsuRI	GGCC	2 cut(s)	260, 408
BsuTUI	ATCGAT	1 cut(s)	440
BtsCI	GGATG	6 cut(s)	115, 225, 766, 816, 834, 1200
BtsIMutI	CAGTG	1 cut(s)	477
Cac8I	GCNNGC	6 cut(s)	365, 406, 410, 519, 664, 1048
CaiI	CAGNNNCTG	1 cut(s)	680
CciI	TCATGA	1 cut(s)	25
CfoI	GCGC	2 cut(s)	202, 1067
ClaI	ATCGAT	1 cut(s)	440
CseI	GACGC	2 cut(s)	411, 428
Csp6I	GTAC	2 cut(s)	295, 1190
CviAII	CATG	7 cut(s)	26, 35, 350, 509, 605, 614, 724
CviQI	GTAC	2 cut(s)	295, 1190
DdeI	CTNAG	6 cut(s)	59, 95, 306, 446, 871, 945
DpnI	GATC	3 cut(s)	386, 886, 1010
DpnII	GATC	3 cut(s)	384, 884, 1008
EaeI	YGGCCR	2 cut(s)	258, 406
Eam1104I	CTCTTC	3 cut(s)	11, 546, 735
EarI	CTCTTC	3 cut(s)	11, 546, 735
Ecl136II	GAGCTC	2 cut(s)	336, 745
Eco105I	TACGTA	1 cut(s)	372
Eco24I	GRGCYC	2 cut(s)	338, 747
Eco47III	AGCGCT	1 cut(s)	201
Eco53kI	GAGCTC	2 cut(s)	336, 745
Eco57I	CTGAAG	3 cut(s)	759, 1184, 1359
EcoICRI	GAGCTC	2 cut(s)	336, 745
EcoT22I	ATGCAT	2 cut(s)	709, 916
EcoT38I	GRGCYC	2 cut(s)	338, 747
FaeI	CATG	7 cut(s)	29, 38, 353, 512, 608, 617, 727
FatI	CATG	7 cut(s)	25, 34, 349, 508, 604, 613, 723
FauI	CCCGC	1 cut(s)	817
FauNDI	CATATG	1 cut(s)	916
FbaI	TGATCA	1 cut(s)	1008
Fnu4HI	GCNGC	4 cut(s)	212, 269, 964, 1295
FokI	GGATG	6 cut(s)	122, 232, 773, 823, 841, 1187
FriOI	GRGCYC	2 cut(s)	338, 747
Fsp4HI	GCNGC	4 cut(s)	212, 269, 964, 1295
FspBI	CTAG	3 cut(s)	609, 807, 1128
GlaI	GCGC	2 cut(s)	201, 1066
GluI	GCNGC	4 cut(s)	212, 269, 964, 1295
HaeII	RGCGCY	1 cut(s)	203
HaeIII	GGCC	2 cut(s)	260, 408
HgaI	GACGC	2 cut(s)	411, 428
HhaI	GCGC	2 cut(s)	202, 1067
Hin1II	CATG	7 cut(s)	29, 38, 353, 512, 608, 617, 727
Hin6I	GCGC	2 cut(s)	200, 1065
HinP1I	GCGC	2 cut(s)	200, 1065
HindIII	AAGCTT	1 cut(s)	1225
HinfI	GANTC	8 cut(s)	40, 64, 88, 100, 490, 1072, 1084, 1178
HphI	GGTGA	1 cut(s)	1145
Hpy166II	GTNNAC	3 cut(s)	172, 789, 1031
Hpy188I	TCNGA	8 cut(s)	331, 427, 594, 754, 804, 889, 1113, 1164
Hpy188III	TCNNGA	3 cut(s)	26, 856, 1088
Hpy8I	GTNNAC	3 cut(s)	172, 789, 1031
Hpy99I	CGWCG	1 cut(s)	427
HpyAV	CCTTC	3 cut(s)	67, 446, 649
HpyCH4III	ACNGT	4 cut(s)	176, 472, 1040, 1094
HpyCH4IV	ACGT	3 cut(s)	371, 1002, 1192
HpyF10VI	GCNNNNNNNGC	2 cut(s)	208, 527
HpyF3I	CTNAG	6 cut(s)	59, 95, 306, 446, 871, 945
HpySE526I	ACGT	3 cut(s)	371, 1002, 1192
Hsp92II	CATG	7 cut(s)	29, 38, 353, 512, 608, 617, 727
HspAI	GCGC	2 cut(s)	200, 1065
Ksp22I	TGATCA	1 cut(s)	1008
Kzo9I	GATC	3 cut(s)	384, 884, 1008
LguI	GCTCTTC	1 cut(s)	735
LmnI	GCTCC	2 cut(s)	341, 466
Lsp1109I	GCAGC	4 cut(s)	198, 255, 950, 1306
LweI	GCATC	7 cut(s)	432, 446, 551, 819, 901, 1161, 1209
MaeI	CTAG	3 cut(s)	609, 807, 1128
MaeII	ACGT	3 cut(s)	371, 1002, 1192
MaeIII	GTNAC	2 cut(s)	250, 472
MalI	GATC	3 cut(s)	386, 886, 1010
MboI	GATC	3 cut(s)	384, 884, 1008
MfeI	CAATTG	2 cut(s)	958, 1244
MflI	RGATCY	1 cut(s)	884
MhlI	GDGCHC	2 cut(s)	338, 747
MlsI	TGGCCA	2 cut(s)	260, 408
MluCI	AATT	9 cut(s)	777, 958, 1119, 1235, 1244, 1283, 1305, 1380, 1400
MluI	ACGCGT	2 cut(s)	392, 420
MluNI	TGGCCA	2 cut(s)	260, 408
MlyI	GAGTC	4 cut(s)	97, 109, 1081, 1187
MmeI	TCCRAC	4 cut(s)	309, 405, 488, 1324
MnlI	CCTC	9 cut(s)	12, 210, 241, 301, 441, 547, 611, 652, 1252
Mox20I	TGGCCA	2 cut(s)	260, 408
Mph1103I	ATGCAT	2 cut(s)	709, 916
MroXI	GAANNNNTTC	2 cut(s)	555, 953
MscI	TGGCCA	2 cut(s)	260, 408
MseI	TTAA	4 cut(s)	9, 141, 414, 1365
MslI	CAYNNNNRTG	1 cut(s)	348
Msp20I	TGGCCA	2 cut(s)	260, 408
MunI	CAATTG	2 cut(s)	958, 1244
Mva1269I	GAATGC	1 cut(s)	343
MvnI	CGCG	3 cut(s)	394, 422, 1067
MwoI	GCNNNNNNNGC	2 cut(s)	208, 527
NdeI	CATATG	1 cut(s)	916
NdeII	GATC	3 cut(s)	384, 884, 1008
NlaIII	CATG	7 cut(s)	29, 38, 353, 512, 608, 617, 727
NlaIV	GGNNCC	1 cut(s)	135
NmeAIII	GCCGAG	1 cut(s)	992
NmuCI	GTSAC	1 cut(s)	472
NsiI	ATGCAT	2 cut(s)	709, 916
NspI	RCATGY	1 cut(s)	38
NspV	TTCGAA	1 cut(s)	189
PagI	TCATGA	1 cut(s)	25
PciSI	GCTCTTC	1 cut(s)	735
PctI	GAATGC	1 cut(s)	343
PdmI	GAANNNNTTC	2 cut(s)	555, 953
PfeI	GAWTC	4 cut(s)	40, 64, 490, 1084
PkrI	GCNGC	4 cut(s)	213, 270, 965, 1296
PleI	GAGTC	4 cut(s)	96, 108, 1080, 1186
PpsI	GAGTC	4 cut(s)	96, 108, 1080, 1186
Ppu21I	YACGTR	2 cut(s)	372, 1193
PshBI	ATTAAT	1 cut(s)	1365
PsiI	TTATAA	1 cut(s)	1269
Psp124BI	GAGCTC	2 cut(s)	338, 747
PspN4I	GGNNCC	1 cut(s)	135
PsrI	GAACNNNNNNTAC	2 cut(s)	1090, 1122
PstI	CTGCAG	2 cut(s)	216, 273
PstNI	CAGNNNCTG	1 cut(s)	680
PsuI	RGATCY	1 cut(s)	884
RsaI	GTAC	2 cut(s)	296, 1191
RsaNI	GTAC	2 cut(s)	295, 1190
RseI	CAYNNNNRTG	1 cut(s)	348
SacI	GAGCTC	2 cut(s)	338, 747
SapI	GCTCTTC	1 cut(s)	735
SaqAI	TTAA	4 cut(s)	9, 141, 414, 1365
SatI	GCNGC	4 cut(s)	212, 269, 964, 1295
Sau3AI	GATC	3 cut(s)	384, 884, 1008
SchI	GAGTC	4 cut(s)	97, 109, 1081, 1187
SduI	GDGCHC	2 cut(s)	338, 747
SfaNI	GCATC	7 cut(s)	432, 446, 551, 819, 901, 1161, 1209
SfcI	CTRYAG	4 cut(s)	212, 269, 468, 1102
SfuI	TTCGAA	1 cut(s)	189
SmiMI	CAYNNNNRTG	1 cut(s)	348
SmlI	CTYRAG	4 cut(s)	668, 833, 856, 992
SmoI	CTYRAG	4 cut(s)	668, 833, 856, 992
SnaBI	TACGTA	1 cut(s)	372
Sse9I	AATT	9 cut(s)	777, 958, 1119, 1235, 1244, 1283, 1305, 1380, 1400
SsiI	CCGC	3 cut(s)	501, 824, 1067
SspI	AATATT	1 cut(s)	589
SspMI	CTAG	3 cut(s)	609, 807, 1128
SstI	GAGCTC	2 cut(s)	338, 747
TaaI	ACNGT	4 cut(s)	176, 472, 1040, 1094
TaiI	ACGT	3 cut(s)	374, 1005, 1195
TaqI	TCGA	4 cut(s)	189, 387, 440, 559
TasI	AATT	9 cut(s)	777, 958, 1119, 1235, 1244, 1283, 1305, 1380, 1400
TfiI	GAWTC	4 cut(s)	40, 64, 490, 1084
Tru1I	TTAA	4 cut(s)	9, 141, 414, 1365
Tru9I	TTAA	4 cut(s)	9, 141, 414, 1365
TscAI	CASTG	1 cut(s)	477
TseFI	GTSAC	1 cut(s)	472
TseI	GCWGC	4 cut(s)	211, 268, 963, 1294
Tsp45I	GTSAC	1 cut(s)	472
TspDTI	ATGAA	5 cut(s)	131, 251, 668, 777, 854
TspRI	CASTG	1 cut(s)	477
VspI	ATTAAT	1 cut(s)	1365
XapI	RAATTY	1 cut(s)	1119
XceI	RCATGY	1 cut(s)	38
XmnI	GAANNNNTTC	2 cut(s)	555, 953
XspI	CTAG	3 cut(s)	609, 807, 1128
Zsp2I	ATGCAT	2 cut(s)	709, 916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.