FvH4_4g02940

subtilisin inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008933
Physical Location & Seq
Forward (+)
2586242 .. 2587580
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02940.t1

Sequence Viewer

Length: 222 bp
ATGGCAACAAGTTCTACTTCTACTGAAACAAAGTGTTCGTGGCCGGAGCTAGTCGGTACCAAAGGAGAGGAAGCAGCAGCCACCATAGTCAAAGAAAATCCATCTGTCAAGGCGCACACAGTGAACGAAGGATCATTTGTGACCTTTGATATGCGTCGCGACAGAGTTCGGGTCTGGATTGATGAGCGTGGGATTGTCACCAAGGCCCCTAAGATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

74

Amino Acids

8.01

Weight (kDa)

7.91

Isoelectric Point (pI)

22.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
potato_inhibit PF00280 11 - 73 2.2e-24 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 56
AccB1I GGYRCC 1 cut(s) 56
AccII CGCG 1 cut(s) 159
AclWI GGATC 1 cut(s) 139
AcoI YGGCCR 1 cut(s) 41
AdeI CACNNNGTG 1 cut(s) 121
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 215
AluBI AGCT 1 cut(s) 49
AluI AGCT 1 cut(s) 49
AlwI GGATC 1 cut(s) 139
AoxI GGCC 2 cut(s) 41, 204
ApeKI GCWGC 2 cut(s) 74, 77
Asp718I GGTACC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 190
BanI GGYRCC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 86, 89
BccI CCATC 1 cut(s) 109
BfaI CTAG 1 cut(s) 50
BisI GCNGC 2 cut(s) 75, 78
BlsI GCNGC 2 cut(s) 76, 79
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 2 cut(s) 58, 207
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 215
BseDI CCNNGG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 215
BseXI GCAGC 2 cut(s) 86, 89
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 43, 206
BshNI GGYRCC 1 cut(s) 56
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 215
BsnI GGCC 2 cut(s) 43, 206
Bsp143I GATC 1 cut(s) 131
Bsp68I TCGCGA 1 cut(s) 159
BspANI GGCC 2 cut(s) 43, 206
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 2 cut(s) 58, 207
BspPI GGATC 1 cut(s) 139
BspT107I GGYRCC 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 201
Bst4CI ACNGT 1 cut(s) 121
BstDEI CTNAG 1 cut(s) 210
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 1 cut(s) 134
BstMBI GATC 1 cut(s) 131
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 86, 89
BsuRI GGCC 2 cut(s) 43, 206
BtsIMutI CAGTG 1 cut(s) 126
BtuMI TCGCGA 1 cut(s) 159
CfoI GCGC 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 205
CseI GACGC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 57
CviJI RGCY 4 cut(s) 43, 49, 80, 206
CviKI_1 RGCY 4 cut(s) 43, 49, 80, 206
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 1 cut(s) 210
DpnI GATC 1 cut(s) 133
DpnII GATC 1 cut(s) 131
DraIII CACNNNGTG 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaiI YATR 2 cut(s) 86, 152
FalI AAGNNNNNCTT 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 75, 78
Fsp4HI GCNGC 2 cut(s) 75, 78
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 114
GluI GCNGC 2 cut(s) 75, 78
HaeIII GGCC 2 cut(s) 43, 206
HapII CCGG 1 cut(s) 44
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 115
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 124
Hpy188III TCNNGA 2 cut(s) 158, 175
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 1 cut(s) 122
HpyCH4III ACNGT 1 cut(s) 121
HpyF3I CTNAG 1 cut(s) 210
HspAI GCGC 1 cut(s) 113
KpnI GGTACC 1 cut(s) 60
Kzo9I GATC 1 cut(s) 131
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 2 cut(s) 57, 160
Lsp1109I GCAGC 2 cut(s) 86, 89
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 139, 196
MalI GATC 1 cut(s) 133
MboI GATC 1 cut(s) 131
MnlI CCTC 1 cut(s) 61
MspI CCGG 1 cut(s) 44
MvnI CGCG 1 cut(s) 159
NdeII GATC 1 cut(s) 131
NlaIV GGNNCC 2 cut(s) 58, 207
NmuCI GTSAC 2 cut(s) 139, 196
NruI TCGCGA 1 cut(s) 159
PkrI GCNGC 2 cut(s) 76, 79
PspN4I GGNNCC 2 cut(s) 58, 207
PspPI GGNCC 1 cut(s) 205
RruI TCGCGA 1 cut(s) 159
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SatI GCNGC 2 cut(s) 75, 78
Sau3AI GATC 1 cut(s) 131
Sau96I GGNCC 1 cut(s) 205
SetI ASST 2 cut(s) 51, 146
SspMI CTAG 1 cut(s) 50
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 121
TscAI CASTG 1 cut(s) 126
TseFI GTSAC 2 cut(s) 139, 196
TseI GCWGC 2 cut(s) 74, 77
Tsp45I GTSAC 2 cut(s) 139, 196
TspRI CASTG 1 cut(s) 126
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.