Rroxscaffold_5G00336840

subtilisin inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008933
Physical Location & Seq
Forward (+)
4679913 .. 4680336
424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00336840.1

Sequence Viewer

Length: 219 bp
ATGGCAAGTTCTATTTCTACTGAAACAAAGAGTTCGTGGCCGGAGCTAGTTGGTACCAAAGGAGAGGAAGCAGCAGCCACCATAATCAAAGAAAACCCTTCTGTCAAAGCCCATACAGTAAACGAAGGATCTTTTGTCACCTTCGATATGCGTAGCGACAGAGTTAGGGTCTGGATTGATGAGCGTGGGATTGTCACTAGGGCGCCTAAGATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

72

Amino Acids

7.88

Weight (kDa)

6.56

Isoelectric Point (pI)

23.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
potato_inhibit PF00280 10 - 72 3.5e-25 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 53, 202
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 1 cut(s) 212
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
AlwI GGATC 1 cut(s) 136
AoxI GGCC 1 cut(s) 38
ApeKI GCWGC 2 cut(s) 71, 74
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 130
BanI GGYRCC 2 cut(s) 53, 202
BbvI GCAGC 2 cut(s) 83, 86
BfaI CTAG 2 cut(s) 47, 198
BfoI RGCGCY 1 cut(s) 206
BisI GCNGC 2 cut(s) 72, 75
BlsI GCNGC 2 cut(s) 73, 76
BmiI GGNNCC 2 cut(s) 55, 204
BsaHI GRCGYC 1 cut(s) 203
Bsc4I CCNNNNNNNGG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 212
BseXI GCAGC 2 cut(s) 83, 86
BshFI GGCC 1 cut(s) 40
BshNI GGYRCC 2 cut(s) 53, 202
BsiSI CCGG 1 cut(s) 41
BslI CCNNNNNNNGG 1 cut(s) 212
BsnI GGCC 1 cut(s) 40
Bsp143I GATC 1 cut(s) 128
BspANI GGCC 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 55, 204
BspPI GGATC 1 cut(s) 136
BspT107I GGYRCC 2 cut(s) 53, 202
BssMI GATC 1 cut(s) 128
BssNI GRCGYC 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 118
BstACI GRCGYC 1 cut(s) 203
BstDEI CTNAG 1 cut(s) 207
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 1 cut(s) 131
BstMBI GATC 1 cut(s) 128
BstV1I GCAGC 2 cut(s) 83, 86
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BsuRI GGCC 1 cut(s) 40
CfoI GCGC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 54
CviJI RGCY 4 cut(s) 40, 46, 77, 110
CviKI_1 RGCY 4 cut(s) 40, 46, 77, 110
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 1 cut(s) 207
DinI GGCGCC 1 cut(s) 204
DpnI GATC 1 cut(s) 130
DpnII GATC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 38
EgeI GGCGCC 1 cut(s) 204
EheI GGCGCC 1 cut(s) 204
FaiI YATR 3 cut(s) 83, 114, 149
Fnu4HI GCNGC 2 cut(s) 72, 75
Fsp4HI GCNGC 2 cut(s) 72, 75
FspBI CTAG 2 cut(s) 47, 198
GlaI GCGC 1 cut(s) 204
GluI GCNGC 2 cut(s) 72, 75
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 1 cut(s) 40
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 203
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 121
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 108, 119, 151
HpyCH4III ACNGT 1 cut(s) 118
HpyF3I CTNAG 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 203
HspAI GCGC 1 cut(s) 203
KasI GGCGCC 1 cut(s) 202
KpnI GGTACC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 128
LmnI GCTCC 1 cut(s) 43
LpnPI CCDG 2 cut(s) 54, 157
Lsp1109I GCAGC 2 cut(s) 83, 86
MaeI CTAG 2 cut(s) 47, 198
MaeIII GTNAC 2 cut(s) 136, 193
MalI GATC 1 cut(s) 130
MboI GATC 1 cut(s) 128
MflI RGATCY 1 cut(s) 128
Mly113I GGCGCC 1 cut(s) 203
MnlI CCTC 1 cut(s) 58
MspI CCGG 1 cut(s) 41
NarI GGCGCC 1 cut(s) 203
NdeII GATC 1 cut(s) 128
NlaIV GGNNCC 2 cut(s) 55, 204
NmuCI GTSAC 2 cut(s) 136, 193
PkrI GCNGC 2 cut(s) 73, 76
PluTI GGCGCC 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 55, 204
PsuI RGATCY 1 cut(s) 128
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SatI GCNGC 2 cut(s) 72, 75
Sau3AI GATC 1 cut(s) 128
SetI ASST 2 cut(s) 48, 143
SfoI GGCGCC 1 cut(s) 204
SgeI CNNG 7 cut(s) 18, 48, 53, 59, 184, 197, 210
SspDI GGCGCC 1 cut(s) 202
SspMI CTAG 2 cut(s) 47, 198
TaaI ACNGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 144
TseFI GTSAC 2 cut(s) 136, 193
TseI GCWGC 2 cut(s) 71, 74
Tsp45I GTSAC 2 cut(s) 136, 193
XspI CTAG 2 cut(s) 47, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.