pycom10g16380

Subtilisin-chymotrypsin inhibitor WSCI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008933
Physical Location & Seq
Reverse (-)
19619889 .. 19620203
315 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g16380.1

Sequence Viewer

Length: 210 bp
ATGGCAGAGTGTCAAGGTAAGAAGTCATGGCCGGAGGTGGTGGGAGAAAATGGGGAAGCTGCGGCGGCAAAGAAAGAGCAAGAGAATCACGGTGTTCGTGCAATCGTGTTACTGGAAGGGTCTGCAACCAGCAAGGACTGGACGTGCGATAGGATCTGGATCTGGGTTAATCAACGCGGCGTCGTCACTAGGGTTCCTCGTGTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

70

Amino Acids

7.63

Weight (kDa)

7.81

Isoelectric Point (pI)

10.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
potato_inhibit PF00280 7 - 69 2.7e-19 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 177
AciI CCGC 3 cut(s) 62, 65, 177
AclWI GGATC 2 cut(s) 161, 167
AcoI YGGCCR 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 203
AjiI CACGTC 1 cut(s) 144
AluBI AGCT 1 cut(s) 59
AluI AGCT 1 cut(s) 59
AlwI GGATC 2 cut(s) 161, 167
AoxI GGCC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 59
BauI CACGAG 1 cut(s) 198
BbvI GCAGC 1 cut(s) 46
BfaI CTAG 1 cut(s) 189
BisI GCNGC 4 cut(s) 60, 63, 66, 178
BlsI GCNGC 4 cut(s) 61, 64, 67, 179
BmgBI CACGTC 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 180
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 117, 143
Bse8I GATNNNNATC 1 cut(s) 158
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 203
BseNI ACTGG 2 cut(s) 117, 143
BseXI GCAGC 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 31
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 203
BsnI GGCC 1 cut(s) 31
Bsp143I GATC 2 cut(s) 153, 159
BspACI CCGC 3 cut(s) 62, 65, 177
BspANI GGCC 1 cut(s) 31
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 195
BspPI GGATC 2 cut(s) 161, 167
BsrI ACTGG 2 cut(s) 117, 143
BssMI GATC 2 cut(s) 153, 159
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 198
Bst2BI CACGAG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 180
BstFNI CGCG 1 cut(s) 177
BstKTI GATC 2 cut(s) 156, 162
BstMBI GATC 2 cut(s) 153, 159
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 46
BstX2I RGATCY 2 cut(s) 153, 159
BstYI RGATCY 2 cut(s) 153, 159
BsuRI GGCC 1 cut(s) 31
BtrI CACGTC 1 cut(s) 144
CseI GACGC 1 cut(s) 169
CviAII CATG 1 cut(s) 27
CviJI RGCY 2 cut(s) 31, 59
CviKI_1 RGCY 2 cut(s) 31, 59
DpnI GATC 2 cut(s) 155, 161
DpnII GATC 2 cut(s) 153, 159
EaeI YGGCCR 1 cut(s) 29
FaeI CATG 1 cut(s) 30
FaiI YATR 1 cut(s) 28
FatI CATG 1 cut(s) 26
Fnu4HI GCNGC 4 cut(s) 60, 63, 66, 178
Fsp4HI GCNGC 4 cut(s) 60, 63, 66, 178
FspBI CTAG 1 cut(s) 189
GluI GCNGC 4 cut(s) 60, 63, 66, 178
HaeIII GGCC 1 cut(s) 31
HapII CCGG 1 cut(s) 32
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 1 cut(s) 30
HinfI GANTC 1 cut(s) 85
HpaII CCGG 1 cut(s) 32
Hpy188III TCNNGA 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 2 cut(s) 101, 125
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 1 cut(s) 30
Kzo9I GATC 2 cut(s) 153, 159
LpnPI CCDG 6 cut(s) 45, 98, 124, 142, 142, 148
Lsp1109I GCAGC 1 cut(s) 46
MaeI CTAG 1 cut(s) 189
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 2 cut(s) 108, 184
MalI GATC 2 cut(s) 155, 161
MboI GATC 2 cut(s) 153, 159
MflI RGATCY 2 cut(s) 153, 159
MnlI CCTC 2 cut(s) 28, 207
MseI TTAA 1 cut(s) 168
MspI CCGG 1 cut(s) 32
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeII GATC 2 cut(s) 153, 159
NlaIII CATG 1 cut(s) 30
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 85
PkrI GCNGC 4 cut(s) 61, 64, 67, 179
PspN4I GGNNCC 1 cut(s) 195
PsuI RGATCY 2 cut(s) 153, 159
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 4 cut(s) 60, 63, 66, 178
Sau3AI GATC 2 cut(s) 153, 159
SetI ASST 4 cut(s) 19, 39, 61, 146
SsiI CCGC 3 cut(s) 62, 65, 177
SspMI CTAG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 1 cut(s) 146
TauI GCSGC 3 cut(s) 65, 68, 180
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 184
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.