Prupe.1G032900_v2.0.a1

subtilisin inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0008933
Physical Location & Seq
Forward (+)
2290158 .. 2291151
994 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G032900.1

Sequence Viewer

Length: 228 bp
ATGGCGAGTAGCACAAGCACTGGTGCAGCAGCAGGAAAGAGTTCATGGCCAGAGCTGGTGGGGACTAAAGGAGAGGAGGCAGTAGCCACTATAATGAAAGAAAATCCATCGCTCAAAGCCCACACAATAAATCAAGGGTCCTTCATCACCATGGATCATCGTCGCGACAGGGTTCGTGTCTGGATTGATGAGCAAGGTGTTGTCACTGCAGCGCCTAAGATAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

76

Amino Acids

7.99

Weight (kDa)

8.18

Isoelectric Point (pI)

22.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 165
AclWI GGATC 1 cut(s) 162
AcoI YGGCCR 1 cut(s) 47
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
AlwI GGATC 1 cut(s) 162
AoxI GGCC 1 cut(s) 47
ApeKI GCWGC 3 cut(s) 26, 29, 209
Asp700I GAANNNNTTC 1 cut(s) 40
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 139
AvaII GGWCC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 49
BbvI GCAGC 3 cut(s) 38, 41, 221
BccI CCATC 1 cut(s) 115
BfmI CTRYAG 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 215
BisI GCNGC 3 cut(s) 27, 30, 210
BlsI GCNGC 3 cut(s) 28, 31, 211
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 25
BseDI CCNNGG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 3 cut(s) 38, 41, 221
BsgI GTGCAG 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 1 cut(s) 49
BslFI GGGAC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 1 cut(s) 49
Bsp143I GATC 1 cut(s) 154
Bsp19I CCATGG 1 cut(s) 150
Bsp68I TCGCGA 1 cut(s) 165
BspANI GGCC 1 cut(s) 49
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 139
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 1 cut(s) 162
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 150
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 1 cut(s) 150
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 1 cut(s) 157
BstMBI GATC 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 3 cut(s) 38, 41, 221
BsuRI GGCC 1 cut(s) 49
BtgI CCRYGG 1 cut(s) 150
BtgZI GCGATG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 18, 204
BtuMI TCGCGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 138
CviAII CATG 2 cut(s) 45, 151
CviJI RGCY 4 cut(s) 49, 55, 86, 119
CviKI_1 RGCY 4 cut(s) 49, 55, 86, 119
DdeI CTNAG 1 cut(s) 216
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 48, 154
FaiI YATR 3 cut(s) 46, 92, 152
FaqI GGGAC 1 cut(s) 76
FatI CATG 2 cut(s) 44, 150
Fnu4HI GCNGC 3 cut(s) 27, 30, 210
Fsp4HI GCNGC 3 cut(s) 27, 30, 210
GlaI GCGC 1 cut(s) 213
GluI GCNGC 3 cut(s) 27, 30, 210
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 1 cut(s) 49
HhaI GCGC 1 cut(s) 214
Hin1II CATG 2 cut(s) 48, 154
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HphI GGTGA 1 cut(s) 139
Hpy188III TCNNGA 2 cut(s) 164, 181
Hpy99I CGWCG 1 cut(s) 165
HpyAV CCTTC 1 cut(s) 151
HpyCH4V TGCA 2 cut(s) 26, 209
HpyF3I CTNAG 1 cut(s) 216
Hsp92II CATG 2 cut(s) 48, 154
HspAI GCGC 1 cut(s) 212
Kzo9I GATC 1 cut(s) 154
LpnPI CCDG 6 cut(s) 6, 18, 41, 63, 154, 166
Lsp1109I GCAGC 3 cut(s) 38, 41, 221
MaeIII GTNAC 1 cut(s) 202
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MlsI TGGCCA 1 cut(s) 49
MluNI TGGCCA 1 cut(s) 49
MnlI CCTC 2 cut(s) 67, 70
Mox20I TGGCCA 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 40
MscI TGGCCA 1 cut(s) 49
MslI CAYNNNNRTG 2 cut(s) 92, 149
Msp20I TGGCCA 1 cut(s) 49
MvnI CGCG 1 cut(s) 165
NcoI CCATGG 1 cut(s) 150
NdeII GATC 1 cut(s) 154
NlaIII CATG 2 cut(s) 48, 154
NlaIV GGNNCC 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 202
NruI TCGCGA 1 cut(s) 165
PdmI GAANNNNTTC 1 cut(s) 40
PkrI GCNGC 3 cut(s) 28, 31, 211
PpuMI RGGWCCY 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 139
PspPI GGNCC 1 cut(s) 138
PspPPI RGGWCCY 1 cut(s) 138
PstI CTGCAG 1 cut(s) 211
RruI TCGCGA 1 cut(s) 165
RseI CAYNNNNRTG 2 cut(s) 92, 149
SatI GCNGC 3 cut(s) 27, 30, 210
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 1 cut(s) 138
SetI ASST 2 cut(s) 57, 199
SfcI CTRYAG 1 cut(s) 207
SinI GGWCC 1 cut(s) 138
SmiMI CAYNNNNRTG 2 cut(s) 92, 149
StyI CCWWGG 1 cut(s) 150
TscAI CASTG 2 cut(s) 25, 211
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 3 cut(s) 26, 29, 209
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 3 cut(s) 33, 110, 133
TspRI CASTG 2 cut(s) 25, 211
VpaK11BI GGWCC 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.