FvH4_4g05520

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006665
Physical Location & Seq
Forward (+)
4773599 .. 4775443
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05520.t1

Sequence Viewer

Length: 1368 bp
ATGGAGGCTAAGCCGCCAAAATCTCCAGCCTACAGAGCTCTACTCAAAGCCGGTCCAAATCTGAAGGCTCTGCAGCAAGTCCACGCACACATTGTAACCTCTGGGTCTCACCGCAGCCGATCTCTCCTTACCAAGCTGATCACTCTGGCATGCGAAGCCGGCTCGATTCTCTACGCTCACAATCTCTTCCTCTCTGTCCCTCACCCGGACTCCTTCCTCTTCAATTCCATTATCAATTCCTCCTCTAAATTCGGCTTCCCTCAGTACACTCTCTCCTTCTATCGCTCCATGCTCGCTTTCGCAATGCCGCCGAGCAGTTACACCTTTACCTCTGTCATCAAGGCCTGCGCTGATCTGTCGGCCTTAAAACTCGGCCAGGGCGTGCATTCACATGTGTTCAGGTTTGGGTGCGGTTCGGAACGGTTTGTTCAGGCAGCTCTTGTTAGCTTGTATGCGAAATGTGGCGAGTTTCGGGTTGCACGGAAGGTGTTTGATGAAATGCCTGGGAGGACTCTTGTCGGTTGGAATTCGATGATTTCGGGGTACGAGCAGAACGGGTTTCCGAGGGAGGCGATTGAGGTGTTTAAGGAGATGAGGGAGTCGGGTGTAGAGCCGGATTCGACTGGTTTTGTGAATGTCTTGGCGGCGTGTTCTCGGTTGGAGAATGATGGTTTGGGGTGTGTGGTGCATGATGAGGTTGTTAGGAGGGGTCTGGAGATTAATGTGCCGCTAGGGACTTCGCTGATTAACATGTATGCTAGATGTGGGGATGTGAGGAAAGCGCGAGAGGTTTTCGAGTGTATGGATGAAAGGAATGTCGTTACGTGGACTGCTATGATTTCGGGTTATGCAACCAATGGTTATGGTAGTCAAGCTATGGAACTCTTTGGCGAAATGAGAGCTCGTGGTCCTAGACCTAACACCGTCACATTTGTGGCAGTCTTGACGGCTTGTGCTCATGCCGGGCTAGTGAAGGAAGGGAGGCAGGCGTATGCAAGCATGAGGCAAGAGTATGGATTAGTGCCCGGAGTGGAGCACCGTGTTTGCCTGGTGGATATGCTTGGGCGTTCTGGGCTTCTTGATGAAGCATATCAGTTCATCAAAGAGCTTAGTCCGGAAGAGGCAGCTCCGCCTATTTGGACTGCCATGCTTGGGGCTTGCAAGATGCATAAGAATACTGATCTTGGAGTAACAGTTGCTGAGCATCTTTTGGAGGTTGAGCCTGAAAATCCAGGCCATTATGTGAATCTATCAAACATATATGCATTGGCAGGTAGGATGGACAGAGTGGAGAAGGTTAGAAACATGATGAAAGAAAGAAGGTTGAGAAAGGGAGTTGGCTATAGTACTATACATGTGAATCAGTAA

Protein Analysis

456

Amino Acids

50.28

Weight (kDa)

8.67

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 172 - 217 1e-09 PPR repeat family
PPR PF01535 175 - 203 2.8e-10 PPR repeat
PPR_2 PF13041 271 - 320 3.2e-13 PPR repeat family
PPR_3 PF13812 272 - 318 5.5e-07 Pentatricopeptide repeat domain
PPR PF01535 274 - 303 3.7e-08 PPR repeat
E_motif PF20431 390 - 451 6.4e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1262
AccII CGCG 1 cut(s) 784
AccIII TCCGGA 1 cut(s) 1114
AciI CCGC 7 cut(s) 14, 112, 308, 411, 644, 728, 1130
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 2 cut(s) 248, 526
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 3 cut(s) 266, 545, 1348
AfiI CCNNNNNNNGG 2 cut(s) 205, 1152
AflIII ACRYGT 3 cut(s) 391, 750, 1354
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 4 cut(s) 375, 502, 1047, 1231
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AleI CACNNNNGTG 1 cut(s) 932
AluBI AGCT 8 cut(s) 38, 136, 437, 447, 875, 902, 1108, 1127
AluI AGCT 8 cut(s) 38, 136, 437, 447, 875, 902, 1108, 1127
Alw21I GWGCWC 4 cut(s) 40, 904, 958, 1038
Alw26I GTCTC 1 cut(s) 111
AlwNI CAGNNNCTG 1 cut(s) 1199
Aor13HI TCCGGA 1 cut(s) 1114
AoxI GGCC 4 cut(s) 342, 360, 373, 1234
ApeKI GCWGC 4 cut(s) 73, 114, 434, 1124
ApoI RAATTY 2 cut(s) 248, 526
AseI ATTAAT 1 cut(s) 720
AspLEI GCGC 2 cut(s) 350, 784
AspS9I GGNCC 2 cut(s) 53, 908
AsuC2I CCSGG 3 cut(s) 206, 964, 1026
AsuHPI GGTGA 2 cut(s) 101, 194
AvaII GGWCC 2 cut(s) 53, 908
BaeGI GKGCMC 1 cut(s) 1026
BanII GRGCYC 2 cut(s) 40, 904
BauI CACGAG 1 cut(s) 903
Bbv12I GWGCWC 4 cut(s) 40, 904, 958, 1038
BbvI GCAGC 4 cut(s) 85, 126, 446, 1136
BccI CCATC 2 cut(s) 662, 1273
BceAI ACGGC 1 cut(s) 963
BciT130I CCWGG 4 cut(s) 377, 504, 1049, 1233
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 3 cut(s) 206, 964, 1026
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 4 cut(s) 731, 759, 912, 968
BfmI CTRYAG 3 cut(s) 31, 71, 1342
BfuAI ACCTGC 1 cut(s) 1262
BisI GCNGC 8 cut(s) 14, 74, 115, 308, 435, 645, 728, 1125
BlpI GCTNAGC 2 cut(s) 9, 1200
BlsI GCNGC 8 cut(s) 15, 75, 116, 309, 436, 646, 729, 1126
BmcAI AGTACT 1 cut(s) 1348
Bme1390I CCNGG 7 cut(s) 206, 377, 504, 964, 1026, 1049, 1233
Bme18I GGWCC 2 cut(s) 53, 908
BmgT120I GGNCC 2 cut(s) 53, 908
BmrFI CCNGG 7 cut(s) 206, 377, 504, 964, 1026, 1049, 1233
BmsI GCATC 2 cut(s) 1155, 1213
BoxI GACNNNNGTC 1 cut(s) 515
BplI GAGNNNNNCTC 2 cut(s) 27, 59
BpmI CTGGAG 2 cut(s) 9, 734
Bpu1102I GCTNAGC 2 cut(s) 9, 1200
BpuMI CCSGG 3 cut(s) 206, 964, 1026
BsaAI YACGTR 1 cut(s) 825
BsaI GGTCTC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 376, 503, 563
BsaWI WCCGGW 1 cut(s) 1114
BsaXI ACNNNNNCTCC 6 cut(s) 194, 224, 257, 287, 1179, 1209
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 1152
Bse118I RCCGGY 2 cut(s) 50, 158
Bse1I ACTGG 1 cut(s) 628
Bse3DI GCAATG 1 cut(s) 309
BseAI TCCGGA 1 cut(s) 1114
BseBI CCWGG 4 cut(s) 377, 504, 1049, 1233
BseDI CCNNGG 3 cut(s) 376, 503, 563
BseGI GGATG 3 cut(s) 775, 811, 1284
BseLI CCNNNNNNNGG 2 cut(s) 205, 1152
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 2 cut(s) 275, 1191
BseNI ACTGG 1 cut(s) 628
BseRI GAGGAG 1 cut(s) 232
BseSI GKGCMC 1 cut(s) 1026
BseXI GCAGC 4 cut(s) 85, 126, 446, 1136
Bsh1236I CGCG 1 cut(s) 784
BshFI GGCC 4 cut(s) 344, 362, 375, 1236
BsiHKAI GWGCWC 4 cut(s) 40, 904, 958, 1038
BsiSI CCGG 7 cut(s) 51, 159, 206, 614, 963, 1026, 1115
BslFI GGGAC 2 cut(s) 182, 748
BslI CCNNNNNNNGG 2 cut(s) 205, 1152
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 2 cut(s) 182, 748
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 4 cut(s) 344, 362, 375, 1236
Bso31I GGTCTC 1 cut(s) 111
Bsp1286I GDGCHC 5 cut(s) 40, 904, 958, 1026, 1038
Bsp13I TCCGGA 1 cut(s) 1114
Bsp143I GATC 4 cut(s) 119, 138, 352, 1180
Bsp1720I GCTNAGC 2 cut(s) 9, 1200
BspACI CCGC 7 cut(s) 14, 112, 308, 411, 644, 728, 1130
BspANI GGCC 4 cut(s) 344, 362, 375, 1236
BspCNI CTCAG 2 cut(s) 274, 1192
BspEI TCCGGA 1 cut(s) 1114
BspFNI CGCG 1 cut(s) 784
BspMAI CTGCAG 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 1262
BspTNI GGTCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 309
BsrFI RCCGGY 2 cut(s) 50, 158
BsrI ACTGG 1 cut(s) 628
BssAI RCCGGY 2 cut(s) 50, 158
BssECI CCNNGG 3 cut(s) 376, 503, 563
BssMI GATC 4 cut(s) 119, 138, 352, 1180
BssSI CACGAG 1 cut(s) 903
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 4 cut(s) 377, 504, 1049, 1233
Bst4CI ACNGT 4 cut(s) 423, 925, 1040, 1195
Bst6I CTCTTC 3 cut(s) 191, 224, 1113
BstBAI YACGTR 1 cut(s) 825
BstC8I GCNNGC 8 cut(s) 151, 160, 294, 346, 383, 987, 997, 1159
BstDEI CTNAG 4 cut(s) 9, 261, 1109, 1200
BstF5I GGATG 3 cut(s) 775, 811, 1284
BstFNI CGCG 1 cut(s) 784
BstHHI GCGC 2 cut(s) 350, 784
BstKTI GATC 4 cut(s) 122, 141, 355, 1183
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 4 cut(s) 119, 138, 352, 1180
BstMWI GCNNNNNNNGC 4 cut(s) 35, 155, 159, 1072
BstNI CCWGG 4 cut(s) 377, 504, 1049, 1233
BstNSI RCATGY 4 cut(s) 153, 395, 754, 1358
BstPAI GACNNNNGTC 1 cut(s) 515
BstSCI CCNGG 7 cut(s) 204, 375, 502, 962, 1024, 1047, 1231
BstSFI CTRYAG 3 cut(s) 31, 71, 1342
BstSLI GKGCMC 1 cut(s) 1026
BstUI CGCG 1 cut(s) 784
BstV1I GCAGC 4 cut(s) 85, 126, 446, 1136
BsuRI GGCC 4 cut(s) 344, 362, 375, 1236
BtsCI GGATG 3 cut(s) 775, 811, 1284
BveI ACCTGC 1 cut(s) 1262
Cac8I GCNNGC 8 cut(s) 151, 160, 294, 346, 383, 987, 997, 1159
CaiI CAGNNNCTG 1 cut(s) 1199
CfoI GCGC 2 cut(s) 350, 784
Cfr10I RCCGGY 2 cut(s) 50, 158
Cfr13I GGNCC 2 cut(s) 53, 908
Csp6I GTAC 3 cut(s) 265, 544, 1347
CviQI GTAC 3 cut(s) 265, 544, 1347
DdeI CTNAG 4 cut(s) 9, 261, 1109, 1200
DpnI GATC 4 cut(s) 121, 140, 354, 1182
DpnII GATC 4 cut(s) 119, 138, 352, 1180
EaeI YGGCCR 1 cut(s) 373
Eam1104I CTCTTC 3 cut(s) 191, 224, 1113
EarI CTCTTC 3 cut(s) 191, 224, 1113
EciI GGCGGA 1 cut(s) 1119
Ecl136II GAGCTC 2 cut(s) 38, 902
Eco147I AGGCCT 1 cut(s) 344
Eco24I GRGCYC 2 cut(s) 40, 904
Eco31I GGTCTC 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 53, 908
Eco53kI GAGCTC 2 cut(s) 38, 902
Eco57I CTGAAG 1 cut(s) 83
EcoICRI GAGCTC 2 cut(s) 38, 902
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 4 cut(s) 375, 502, 1047, 1231
EcoT22I ATGCAT 2 cut(s) 1170, 1267
EcoT38I GRGCYC 2 cut(s) 40, 904
FaqI GGGAC 2 cut(s) 182, 748
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 8 cut(s) 14, 74, 115, 308, 435, 645, 728, 1125
FokI GGATG 3 cut(s) 782, 818, 1291
FriOI GRGCYC 2 cut(s) 40, 904
Fsp4HI GCNGC 8 cut(s) 14, 74, 115, 308, 435, 645, 728, 1125
FspBI CTAG 4 cut(s) 731, 759, 912, 968
GlaI GCGC 2 cut(s) 349, 783
GluI GCNGC 8 cut(s) 14, 74, 115, 308, 435, 645, 728, 1125
GsuI CTGGAG 2 cut(s) 9, 734
HaeIII GGCC 4 cut(s) 344, 362, 375, 1236
HapII CCGG 7 cut(s) 51, 159, 206, 614, 963, 1026, 1115
HhaI GCGC 2 cut(s) 350, 784
Hin6I GCGC 2 cut(s) 348, 782
HinP1I GCGC 2 cut(s) 348, 782
HinfI GANTC 7 cut(s) 166, 209, 511, 599, 617, 1246, 1360
HpaII CCGG 7 cut(s) 51, 159, 206, 614, 963, 1026, 1115
HphI GGTGA 2 cut(s) 101, 194
Hpy166II GTNNAC 3 cut(s) 82, 267, 828
Hpy188I TCNGA 3 cut(s) 63, 418, 564
Hpy188III TCNNGA 4 cut(s) 713, 943, 1079, 1115
Hpy8I GTNNAC 3 cut(s) 82, 267, 828
HpyAV CCTTC 8 cut(s) 58, 223, 286, 478, 967, 971, 1288, 1314
HpyCH4III ACNGT 4 cut(s) 423, 925, 1040, 1195
HpyCH4IV ACGT 1 cut(s) 824
HpyCH4V TGCA 9 cut(s) 73, 385, 479, 688, 851, 995, 1161, 1168, 1265
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 155, 159, 1072
HpyF3I CTNAG 4 cut(s) 9, 261, 1109, 1200
HpySE526I ACGT 1 cut(s) 824
HspAI GCGC 2 cut(s) 348, 782
Kpn2I TCCGGA 1 cut(s) 1114
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 4 cut(s) 119, 138, 352, 1180
LmnI GCTCC 3 cut(s) 290, 1033, 1132
Lsp1109I GCAGC 4 cut(s) 85, 126, 446, 1136
LweI GCATC 2 cut(s) 1155, 1213
MaeI CTAG 4 cut(s) 731, 759, 912, 968
MaeII ACGT 1 cut(s) 824
MaeIII GTNAC 5 cut(s) 94, 317, 820, 925, 1189
MalI GATC 4 cut(s) 121, 140, 354, 1182
MboI GATC 4 cut(s) 119, 138, 352, 1180
MboII GAAGA 3 cut(s) 178, 211, 1130
MhlI GDGCHC 5 cut(s) 40, 904, 958, 1026, 1038
MluCI AATT 4 cut(s) 223, 235, 248, 526
MlyI GAGTC 3 cut(s) 203, 505, 608
MmeI TCCRAC 2 cut(s) 503, 639
Mph1103I ATGCAT 2 cut(s) 1170, 1267
MroI TCCGGA 1 cut(s) 1114
MroNI GCCGGC 1 cut(s) 158
MseI TTAA 4 cut(s) 365, 585, 720, 747
MslI CAYNNNNRTG 2 cut(s) 390, 932
MspI CCGG 7 cut(s) 51, 159, 206, 614, 963, 1026, 1115
MspR9I CCNGG 7 cut(s) 206, 377, 504, 964, 1026, 1049, 1233
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 4 cut(s) 377, 504, 1049, 1233
MvnI CGCG 1 cut(s) 784
MwoI GCNNNNNNNGC 4 cut(s) 35, 155, 159, 1072
NaeI GCCGGC 1 cut(s) 160
NciI CCSGG 3 cut(s) 206, 964, 1026
NdeII GATC 4 cut(s) 119, 138, 352, 1180
NgoMIV GCCGGC 1 cut(s) 158
NmeAIII GCCGAG 2 cut(s) 336, 351
NmuCI GTSAC 1 cut(s) 925
NsiI ATGCAT 2 cut(s) 1170, 1267
NspI RCATGY 4 cut(s) 153, 395, 754, 1358
OliI CACNNNNGTG 1 cut(s) 932
PaeI GCATGC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 344
PciI ACATGT 3 cut(s) 391, 750, 1354
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PctI GAATGC 1 cut(s) 385
PdiI GCCGGC 1 cut(s) 160
PfeI GAWTC 4 cut(s) 166, 617, 1246, 1360
PkrI GCNGC 8 cut(s) 15, 75, 116, 309, 436, 646, 729, 1126
PleI GAGTC 3 cut(s) 203, 505, 607
PpsI GAGTC 3 cut(s) 203, 505, 607
Ppu21I YACGTR 1 cut(s) 825
PscI ACATGT 3 cut(s) 391, 750, 1354
PshAI GACNNNNGTC 1 cut(s) 515
PshBI ATTAAT 1 cut(s) 720
Psp124BI GAGCTC 2 cut(s) 40, 904
Psp6I CCWGG 4 cut(s) 375, 502, 1047, 1231
PspGI CCWGG 4 cut(s) 375, 502, 1047, 1231
PspPI GGNCC 2 cut(s) 53, 908
PstI CTGCAG 1 cut(s) 75
PstNI CAGNNNCTG 1 cut(s) 1199
RsaI GTAC 3 cut(s) 266, 545, 1348
RsaNI GTAC 3 cut(s) 265, 544, 1347
RseI CAYNNNNRTG 2 cut(s) 390, 932
SacI GAGCTC 2 cut(s) 40, 904
SaqAI TTAA 4 cut(s) 365, 585, 720, 747
SatI GCNGC 8 cut(s) 14, 74, 115, 308, 435, 645, 728, 1125
Sau3AI GATC 4 cut(s) 119, 138, 352, 1180
Sau96I GGNCC 2 cut(s) 53, 908
ScaI AGTACT 1 cut(s) 1348
SchI GAGTC 3 cut(s) 203, 505, 608
ScrFI CCNGG 7 cut(s) 206, 377, 504, 964, 1026, 1049, 1233
SduI GDGCHC 5 cut(s) 40, 904, 958, 1026, 1038
SfaNI GCATC 2 cut(s) 1155, 1213
SfcI CTRYAG 3 cut(s) 31, 71, 1342
SinI GGWCC 2 cut(s) 53, 908
SmiMI CAYNNNNRTG 2 cut(s) 390, 932
SphI GCATGC 1 cut(s) 153
Sse9I AATT 4 cut(s) 223, 235, 248, 526
SseBI AGGCCT 1 cut(s) 344
SsiI CCGC 7 cut(s) 14, 112, 308, 411, 644, 728, 1130
SspMI CTAG 4 cut(s) 731, 759, 912, 968
SstI GAGCTC 2 cut(s) 40, 904
StuI AGGCCT 1 cut(s) 344
StyD4I CCNGG 7 cut(s) 204, 375, 502, 962, 1024, 1047, 1231
TaaI ACNGT 4 cut(s) 423, 925, 1040, 1195
TaiI ACGT 1 cut(s) 827
TaqI TCGA 4 cut(s) 164, 530, 620, 795
TasI AATT 4 cut(s) 223, 235, 248, 526
TatI WGTACW 2 cut(s) 264, 1346
TauI GCSGC 4 cut(s) 16, 310, 647, 730
TfiI GAWTC 4 cut(s) 166, 617, 1246, 1360
Tru1I TTAA 4 cut(s) 365, 585, 720, 747
Tru9I TTAA 4 cut(s) 365, 585, 720, 747
TseFI GTSAC 1 cut(s) 925
TseI GCWGC 4 cut(s) 73, 114, 434, 1124
Tsp45I GTSAC 1 cut(s) 925
TspDTI ATGAA 5 cut(s) 510, 822, 1087, 1098, 1325
TspGWI ACGGA 1 cut(s) 496
VpaK11BI GGWCC 2 cut(s) 53, 908
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 2 cut(s) 248, 526
XceI RCATGY 4 cut(s) 153, 395, 754, 1358
XspI CTAG 4 cut(s) 731, 759, 912, 968
ZrmI AGTACT 1 cut(s) 1348
Zsp2I ATGCAT 2 cut(s) 1170, 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.