Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0006665

Physical Location & Seq

Genomic Coordinates Forward (+)

6251036 .. 6252787

Gene Structure 1752 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031564

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGGAAACCAAACACAGCGCCCGGCCAAAATCTCCAGCCTACAAAGCTCTACTCAAAGCCGGTCCAAATCTGAAGCCTCTTCAACAAGTCCACGCCCACATTATCGTCTCTGGATCTCACCGCAGCCGATCACTCCTCACCAAGCTCATCACTCTGGCTTGCGAAGCCGGCTCGATTCTCTACATCCACAACCTCTTCCTCTCTGTTCCTCACCCCGACTCCTTCCTCTTCAATTCCATAATCAAAACATCCTCTAAGTTCGGCTTCCCGCAACACACTCTCTTCTTCTATCACTCCATGCTCGGTTTTCGAAGCCCGCCGAGTAGTTACACCTTTACCTCTGTGATCAAGGCCTGCGCCGATCTAAGGGCTCTGAGACTCGCCAGGGGTGTGCATTCACATGTAGTGGTGTGTGGGTGTGGGTCGGAGCCGTTTGTTCAGGCGGCTCTTGTTAGCTTGTATTCGAAATGTGGGGAGTTTGGGGTTGCGCGGAAGGTGTTCGATGAAATGCCTGAGAGGAGTGTTGTGGGTTGGAACTCGATGATTTCGGGGTACGAGCAGAACGGGTTTTCGAGAGAGGCGATTGAGGTGTTTAATGAGATGAGGGAGGCGGGTGTTGAGCCGGATTCGACTGGTTTCGTGAATGTTTTGGCGGCGTGTTCTCAGTTGGGAGATGTTGGTTTAGGGTGTTCGGTGCATGATGAGGTTGTCAGGAAGGGGATAGAAGTGAATGTGGCTCTAGGGACTTCGCTGATTAACATGTATGCTAGGTGTGGGGATGTGAGCAAAGCGCGAGAAGTTTTCGATTGTATGGATCAAAGGAATGTTATTACTTGGACTGCTATGATTTCGGGGTACGCAACGAATGGTTATGGTAGTCAAGCTATGGAGCTTTTTGGCGAAATGAGAGCTCGTGGTCCTAGTCCTAACACTGTGACATTTGTTGCAGTCTTGGCGGCTTGTGCTCATGCCGGGCTAGTGAAAGAAGGGAGGCAGGCATATGCAAGCATGAGGCAAGAGTATGGATTAGTGCCGGGAGTGGAGCACCGTGTTTGCCTGGTTGATATGCTTGGGCGTTCTGGGCTTCTCGATGAAGCATATCAGTTCATCAAAGAGCTTAGCCCGGGAGAGGCAGCTCCACCTCTTTGGACTGCCATGCTTGGGGCTTGCAAGATGCATAAGAATACTGATCTCGGAGTCACAGTTGCTGAGCATCTTTTGGAGATTGAGCCAGAAAATCCAGGCCATTATGTGATGCTTTCGAACATATATGCAGTGGCGGGGTATGCACCAGCACCGGAGTTAGTGATGCATGAAGTGGAAGAAGAAGAGAGGGAATATGCCCTTAGATATCACAGTGAGAAGCTTGCAATTGCATATGGGCTAATGAAAACCAAGGATGGTGAGGCCATTAGGATTGTAAAGAACCTCCGAATGTGTGAGGACTGCCACTCGGCTATTAAGTTTATCTCAGTTGTTACCAAAAGAGAGATTATTGTTCGGGATAAGCTTCGCTTCCACCATTTCAAATATGGCTCCTGTTCATGCCTGGATTATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

521

Amino Acids

57.44

Weight (kDa)

7.05

Isoelectric Point (pI)

49.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	174 - 217	2.4e-10	PPR repeat family
PPR	PF01535	178 - 206	3.4e-09	PPR repeat
PPR_2	PF13041	274 - 323	3e-12	PPR repeat family
PPR_3	PF13812	274 - 321	8.4e-07	Pentatricopeptide repeat domain
PPR	PF01535	277 - 306	9.4e-08	PPR repeat
E_motif	PF20431	393 - 439	7.6e-08	E motif
DYW_deaminase	PF14432	428 - 520	6.8e-39	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006665)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33760
fragaria_vesca	FvH4_4g05520
malus_domestica	MD10G1295100.v1.1
prunus_persica	Prupe.4G047100_v2.0.a1
pyrus_communis	pycom10g24690
rosa_chinensis	RchiOBHm_Chr5g0008361
rosa_laevigata	RLG00000031504 RLG00000031508 RLG00000031509 RLG00000031512 RLG00000031518 RLG00000031519 RLG00000031564
rosa_multiflora	Rmu_sc0008805.1_g000001
rosa_roxburghii	Rroxscaffold_1G00055520 Rroxscaffold_1G00068130 Rroxscaffold_1G00068520
rosa_rugosa	Rorug04G0435500
rosa_samantha	Rh5AG059900 Rh5AG060000 Rh5AG064000 Rh5BG060900 Rh5CG070900 Rh6CG126900
rosa_wichuraiana	Rw5G005700 Rw5G006030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	490, 793
AciI	CCGC	9 cut(s)	121, 269, 317, 443, 490, 611, 653, 956, 1280
AclWI	GGATC	2 cut(s)	121, 822
AcoI	YGGCCR	1 cut(s)	23
AcuI	CTGAAG	1 cut(s)	92
AfaI	GTAC	2 cut(s)	554, 857
AfiI	CCNNNNNNNGG	3 cut(s)	366, 1129, 1161
AflIII	ACRYGT	2 cut(s)	400, 759
AgsI	TTSAA	3 cut(s)	83, 232, 1528
AjnI	CCWGG	4 cut(s)	383, 1056, 1240, 1548
Alw21I	GWGCWC	3 cut(s)	913, 967, 1047
Alw26I	GTCTC	2 cut(s)	112, 370
AlwI	GGATC	2 cut(s)	121, 822
AlwNI	CAGNNNCTG	1 cut(s)	1208
Ama87I	CYCGRG	1 cut(s)	1123
AoxI	GGCC	4 cut(s)	23, 351, 1243, 1407
ApeKI	GCWGC	2 cut(s)	123, 1133
Asp700I	GAANNNNTTC	1 cut(s)	497
AspLEI	GCGC	4 cut(s)	20, 359, 490, 793
AspS9I	GGNCC	2 cut(s)	62, 917
AsuC2I	CCSGG	5 cut(s)	22, 973, 1035, 1124, 1125
AsuHPI	GGTGA	4 cut(s)	110, 130, 203, 1415
AsuII	TTCGAA	3 cut(s)	310, 464, 1262
AvaI	CYCGRG	1 cut(s)	1123
AvaII	GGWCC	2 cut(s)	62, 917
BanII	GRGCYC	2 cut(s)	373, 913
BauI	CACGAG	1 cut(s)	912
Bbv12I	GWGCWC	3 cut(s)	913, 967, 1047
BbvI	GCAGC	2 cut(s)	135, 1145
BccI	CCATC	1 cut(s)	1394
BceAI	ACGGC	1 cut(s)	415
BciT130I	CCWGG	4 cut(s)	385, 1058, 1242, 1550
BclI	TGATCA	1 cut(s)	345
BcnI	CCSGG	5 cut(s)	22, 973, 1035, 1124, 1125
BcoDI	GTCTC	2 cut(s)	112, 370
BfaI	CTAG	4 cut(s)	740, 768, 921, 977
BfoI	RGCGCY	1 cut(s)	21
BisI	GCNGC	5 cut(s)	124, 444, 654, 957, 1134
BlpI	GCTNAGC	2 cut(s)	1118, 1209
BlsI	GCNGC	5 cut(s)	125, 445, 655, 958, 1135
Bme1390I	CCNGG	9 cut(s)	22, 385, 973, 1035, 1058, 1124, 1125, 1242, 1550
Bme18I	GGWCC	2 cut(s)	62, 917
BmeT110I	CYCGRG	1 cut(s)	1123
BmgT120I	GGNCC	2 cut(s)	62, 917
BmiI	GGNNCC	2 cut(s)	429, 1537
BmrFI	CCNGG	9 cut(s)	22, 385, 973, 1035, 1058, 1124, 1125, 1242, 1550
BmsI	GCATC	4 cut(s)	1164, 1222, 1245, 1299
BpmI	CTGGAG	1 cut(s)	18
Bpu1102I	GCTNAGC	2 cut(s)	1118, 1209
Bpu14I	TTCGAA	3 cut(s)	310, 464, 1262
BpuMI	CCSGG	5 cut(s)	22, 973, 1035, 1124, 1125
BsaJI	CCNNGG	3 cut(s)	384, 1123, 1395
BsaWI	WCCGGW	1 cut(s)	1297
BsaXI	ACNNNNNCTCC	4 cut(s)	203, 233, 1188, 1218
Bsc4I	CCNNNNNNNGG	3 cut(s)	366, 1129, 1161
Bse118I	RCCGGY	2 cut(s)	59, 167
Bse1I	ACTGG	1 cut(s)	637
BseBI	CCWGG	4 cut(s)	385, 1058, 1242, 1550
BseDI	CCNNGG	3 cut(s)	384, 1123, 1395
BseGI	GGATG	4 cut(s)	183, 248, 784, 1405
BseLI	CCNNNNNNNGG	3 cut(s)	366, 1129, 1161
BseMII	CTCAG	5 cut(s)	365, 504, 677, 1200, 1485
BseNI	ACTGG	1 cut(s)	637
BseRI	GAGGAG	2 cut(s)	125, 532
BseXI	GCAGC	2 cut(s)	135, 1145
Bsh1236I	CGCG	2 cut(s)	490, 793
BshFI	GGCC	4 cut(s)	25, 353, 1245, 1409
BsiHKAI	GWGCWC	3 cut(s)	913, 967, 1047
BsiHKCI	CYCGRG	1 cut(s)	1123
BsiSI	CCGG	8 cut(s)	22, 60, 168, 623, 972, 1034, 1124, 1298
BslFI	GGGAC	1 cut(s)	757
BslI	CCNNNNNNNGG	3 cut(s)	366, 1129, 1161
BsmAI	GTCTC	2 cut(s)	112, 370
BsmBI	CGTCTC	1 cut(s)	112
BsmFI	GGGAC	1 cut(s)	757
BsmI	GAATGC	1 cut(s)	394
BsnI	GGCC	4 cut(s)	25, 353, 1245, 1409
BsoBI	CYCGRG	1 cut(s)	1123
Bsp119I	TTCGAA	3 cut(s)	310, 464, 1262
Bsp1286I	GDGCHC	4 cut(s)	373, 913, 967, 1047
Bsp143I	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
Bsp1720I	GCTNAGC	2 cut(s)	1118, 1209
BspACI	CCGC	9 cut(s)	121, 269, 317, 443, 490, 611, 653, 956, 1280
BspANI	GGCC	4 cut(s)	25, 353, 1245, 1409
BspCNI	CTCAG	5 cut(s)	366, 505, 676, 1201, 1484
BspFNI	CGCG	2 cut(s)	490, 793
BspLI	GGNNCC	2 cut(s)	429, 1537
BspPI	GGATC	2 cut(s)	121, 822
BspT104I	TTCGAA	3 cut(s)	310, 464, 1262
BsrFI	RCCGGY	2 cut(s)	59, 167
BsrI	ACTGG	1 cut(s)	637
BssAI	RCCGGY	2 cut(s)	59, 167
BssECI	CCNNGG	3 cut(s)	384, 1123, 1395
BssMI	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
BssSI	CACGAG	1 cut(s)	912
BssT1I	CCWWGG	1 cut(s)	1395
Bst2BI	CACGAG	1 cut(s)	912
Bst2UI	CCWGG	4 cut(s)	385, 1058, 1242, 1550
Bst4CI	ACNGT	4 cut(s)	934, 1049, 1204, 1358
Bst6I	CTCTTC	5 cut(s)	84, 200, 233, 287, 1323
BstBI	TTCGAA	3 cut(s)	310, 464, 1262
BstC8I	GCNNGC	8 cut(s)	160, 169, 317, 355, 996, 1006, 1168, 1368
BstDEI	CTNAG	9 cut(s)	255, 365, 374, 513, 663, 1118, 1209, 1346, 1471
BstF5I	GGATG	4 cut(s)	183, 248, 784, 1405
BstFNI	CGCG	2 cut(s)	490, 793
BstH2I	RGCGCY	1 cut(s)	21
BstHHI	GCGC	4 cut(s)	20, 359, 490, 793
BstKTI	GATC	6 cut(s)	116, 131, 348, 364, 817, 1192
BstMAI	GTCTC	2 cut(s)	112, 370
BstMBI	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
BstMWI	GCNNNNNNNGC	7 cut(s)	44, 164, 168, 953, 962, 1081, 1286
BstNI	CCWGG	4 cut(s)	385, 1058, 1242, 1550
BstNSI	RCATGY	2 cut(s)	404, 763
BstSCI	CCNGG	9 cut(s)	20, 383, 971, 1033, 1056, 1122, 1123, 1240, 1548
BstUI	CGCG	2 cut(s)	490, 793
BstV1I	GCAGC	2 cut(s)	135, 1145
BstX2I	RGATCY	1 cut(s)	113
BstXI	CCANNNNNNTGG	1 cut(s)	1146
BstYI	RGATCY	1 cut(s)	113
BsuRI	GGCC	4 cut(s)	25, 353, 1245, 1409
BtsCI	GGATG	4 cut(s)	183, 248, 784, 1405
BtsI	GCAGTG	1 cut(s)	1281
BtsIMutI	CAGTG	3 cut(s)	930, 1281, 1363
Cac8I	GCNNGC	8 cut(s)	160, 169, 317, 355, 996, 1006, 1168, 1368
CaiI	CAGNNNCTG	1 cut(s)	1208
CfoI	GCGC	4 cut(s)	20, 359, 490, 793
Cfr10I	RCCGGY	2 cut(s)	59, 167
Cfr13I	GGNCC	2 cut(s)	62, 917
Cfr9I	CCCGGG	1 cut(s)	1123
Csp6I	GTAC	2 cut(s)	553, 856
CviAII	CATG	9 cut(s)	298, 401, 698, 760, 968, 1009, 1156, 1313, 1545
CviQI	GTAC	2 cut(s)	553, 856
DdeI	CTNAG	9 cut(s)	255, 365, 374, 513, 663, 1118, 1209, 1346, 1471
DpnI	GATC	6 cut(s)	115, 130, 347, 363, 816, 1191
DpnII	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
EaeI	YGGCCR	1 cut(s)	23
Eam1104I	CTCTTC	5 cut(s)	84, 200, 233, 287, 1323
EarI	CTCTTC	5 cut(s)	84, 200, 233, 287, 1323
Ecl136II	GAGCTC	1 cut(s)	911
Eco130I	CCWWGG	1 cut(s)	1395
Eco147I	AGGCCT	1 cut(s)	353
Eco24I	GRGCYC	2 cut(s)	373, 913
Eco32I	GATATC	1 cut(s)	1352
Eco47I	GGWCC	2 cut(s)	62, 917
Eco53kI	GAGCTC	1 cut(s)	911
Eco57I	CTGAAG	1 cut(s)	92
Eco88I	CYCGRG	1 cut(s)	1123
EcoICRI	GAGCTC	1 cut(s)	911
EcoRII	CCWGG	4 cut(s)	383, 1056, 1240, 1548
EcoRV	GATATC	1 cut(s)	1352
EcoT14I	CCWWGG	1 cut(s)	1395
EcoT22I	ATGCAT	2 cut(s)	1179, 1314
EcoT38I	GRGCYC	2 cut(s)	373, 913
ErhI	CCWWGG	1 cut(s)	1395
Esp3I	CGTCTC	1 cut(s)	112
FaeI	CATG	9 cut(s)	301, 404, 701, 763, 971, 1012, 1159, 1316, 1548
FalI	AAGNNNNNCTT	4 cut(s)	248, 280, 1499, 1531
FaqI	GGGAC	1 cut(s)	757
FatI	CATG	9 cut(s)	297, 400, 697, 759, 967, 1008, 1155, 1312, 1544
FauI	CCCGC	4 cut(s)	276, 324, 604, 1273
FauNDI	CATATG	2 cut(s)	1000, 1378
FbaI	TGATCA	1 cut(s)	345
Fnu4HI	GCNGC	5 cut(s)	124, 444, 654, 957, 1134
FokI	GGATG	4 cut(s)	170, 235, 791, 1412
FriOI	GRGCYC	2 cut(s)	373, 913
Fsp4HI	GCNGC	5 cut(s)	124, 444, 654, 957, 1134
FspBI	CTAG	4 cut(s)	740, 768, 921, 977
GlaI	GCGC	4 cut(s)	19, 358, 489, 792
GluI	GCNGC	5 cut(s)	124, 444, 654, 957, 1134
GsuI	CTGGAG	1 cut(s)	18
HaeII	RGCGCY	1 cut(s)	21
HaeIII	GGCC	4 cut(s)	25, 353, 1245, 1409
HapII	CCGG	8 cut(s)	22, 60, 168, 623, 972, 1034, 1124, 1298
HhaI	GCGC	4 cut(s)	20, 359, 490, 793
Hin1II	CATG	9 cut(s)	301, 404, 701, 763, 971, 1012, 1159, 1316, 1548
Hin6I	GCGC	4 cut(s)	18, 357, 488, 791
HinP1I	GCGC	4 cut(s)	18, 357, 488, 791
HindIII	AAGCTT	2 cut(s)	1364, 1508
HinfI	GANTC	5 cut(s)	175, 218, 378, 626, 1197
HpaII	CCGG	8 cut(s)	22, 60, 168, 623, 972, 1034, 1124, 1298
HphI	GGTGA	4 cut(s)	110, 130, 203, 1415
Hpy166II	GTNNAC	1 cut(s)	91
Hpy188I	TCNGA	5 cut(s)	72, 375, 427, 1196, 1433
Hpy188III	TCNNGA	6 cut(s)	111, 573, 640, 712, 1088, 1502
Hpy8I	GTNNAC	1 cut(s)	91
HpyAV	CCTTC	4 cut(s)	232, 487, 709, 980
HpyCH4III	ACNGT	4 cut(s)	934, 1049, 1204, 1358
HpyF10VI	GCNNNNNNNGC	7 cut(s)	44, 164, 168, 953, 962, 1081, 1286
HpyF3I	CTNAG	9 cut(s)	255, 365, 374, 513, 663, 1118, 1209, 1346, 1471
Hsp92II	CATG	9 cut(s)	301, 404, 701, 763, 971, 1012, 1159, 1316, 1548
HspAI	GCGC	4 cut(s)	18, 357, 488, 791
KroI	GCCGGC	1 cut(s)	167
KroNI	GCCGGC	1 cut(s)	169
Ksp22I	TGATCA	1 cut(s)	345
Kzo9I	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
LmnI	GCTCC	5 cut(s)	427, 889, 1042, 1141, 1541
Lsp1109I	GCAGC	2 cut(s)	135, 1145
LweI	GCATC	4 cut(s)	1164, 1222, 1245, 1299
MaeI	CTAG	4 cut(s)	740, 768, 921, 977
MaeIII	GTNAC	4 cut(s)	326, 934, 1198, 1477
MalI	GATC	6 cut(s)	115, 130, 347, 363, 816, 1191
MboI	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
MboII	GAAGA	8 cut(s)	71, 187, 220, 274, 277, 1334, 1337, 1340
MfeI	CAATTG	1 cut(s)	1371
MflI	RGATCY	1 cut(s)	113
MhlI	GDGCHC	4 cut(s)	373, 913, 967, 1047
MluCI	AATT	2 cut(s)	232, 1371
MlyI	GAGTC	3 cut(s)	212, 372, 1206
MmeI	TCCRAC	2 cut(s)	405, 512
Mph1103I	ATGCAT	2 cut(s)	1179, 1314
MroNI	GCCGGC	1 cut(s)	167
MroXI	GAANNNNTTC	1 cut(s)	497
MseI	TTAA	3 cut(s)	594, 756, 1461
MslI	CAYNNNNRTG	1 cut(s)	399
MspI	CCGG	8 cut(s)	22, 60, 168, 623, 972, 1034, 1124, 1298
MspR9I	CCNGG	9 cut(s)	22, 385, 973, 1035, 1058, 1124, 1125, 1242, 1550
MunI	CAATTG	1 cut(s)	1371
Mva1269I	GAATGC	1 cut(s)	394
MvaI	CCWGG	4 cut(s)	385, 1058, 1242, 1550
MvnI	CGCG	2 cut(s)	490, 793
MwoI	GCNNNNNNNGC	7 cut(s)	44, 164, 168, 953, 962, 1081, 1286
NaeI	GCCGGC	1 cut(s)	169
NciI	CCSGG	5 cut(s)	22, 973, 1035, 1124, 1125
NdeI	CATATG	2 cut(s)	1000, 1378
NdeII	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
NgoMIV	GCCGGC	1 cut(s)	167
NlaIII	CATG	9 cut(s)	301, 404, 701, 763, 971, 1012, 1159, 1316, 1548
NlaIV	GGNNCC	2 cut(s)	429, 1537
NmeAIII	GCCGAG	2 cut(s)	345, 1433
NmuCI	GTSAC	2 cut(s)	934, 1198
NsiI	ATGCAT	2 cut(s)	1179, 1314
NspI	RCATGY	2 cut(s)	404, 763
NspV	TTCGAA	3 cut(s)	310, 464, 1262
PceI	AGGCCT	1 cut(s)	353
PciI	ACATGT	2 cut(s)	400, 759
PcsI	WCGNNNNNNNCGW	1 cut(s)	578
PctI	GAATGC	1 cut(s)	394
PdiI	GCCGGC	1 cut(s)	169
PdmI	GAANNNNTTC	1 cut(s)	497
PfeI	GAWTC	2 cut(s)	175, 626
PkrI	GCNGC	5 cut(s)	125, 445, 655, 958, 1135
PleI	GAGTC	3 cut(s)	212, 372, 1205
PpsI	GAGTC	3 cut(s)	212, 372, 1205
PscI	ACATGT	2 cut(s)	400, 759
Psp124BI	GAGCTC	1 cut(s)	913
Psp6I	CCWGG	4 cut(s)	383, 1056, 1240, 1548
PspGI	CCWGG	4 cut(s)	383, 1056, 1240, 1548
PspN4I	GGNNCC	2 cut(s)	429, 1537
PspPI	GGNCC	2 cut(s)	62, 917
PstNI	CAGNNNCTG	1 cut(s)	1208
PsuI	RGATCY	1 cut(s)	113
RsaI	GTAC	2 cut(s)	554, 857
RsaNI	GTAC	2 cut(s)	553, 856
RseI	CAYNNNNRTG	1 cut(s)	399
SacI	GAGCTC	1 cut(s)	913
SaqAI	TTAA	3 cut(s)	594, 756, 1461
SatI	GCNGC	5 cut(s)	124, 444, 654, 957, 1134
Sau3AI	GATC	6 cut(s)	113, 128, 345, 361, 814, 1189
Sau96I	GGNCC	2 cut(s)	62, 917
SchI	GAGTC	3 cut(s)	212, 372, 1206
ScrFI	CCNGG	9 cut(s)	22, 385, 973, 1035, 1058, 1124, 1125, 1242, 1550
SduI	GDGCHC	4 cut(s)	373, 913, 967, 1047
SfaNI	GCATC	4 cut(s)	1164, 1222, 1245, 1299
SfuI	TTCGAA	3 cut(s)	310, 464, 1262
SinI	GGWCC	2 cut(s)	62, 917
SmaI	CCCGGG	1 cut(s)	1125
SmiMI	CAYNNNNRTG	1 cut(s)	399
Sse9I	AATT	2 cut(s)	232, 1371
SseBI	AGGCCT	1 cut(s)	353
SsiI	CCGC	9 cut(s)	121, 269, 317, 443, 490, 611, 653, 956, 1280
SspMI	CTAG	4 cut(s)	740, 768, 921, 977
SstI	GAGCTC	1 cut(s)	913
StuI	AGGCCT	1 cut(s)	353
StyD4I	CCNGG	9 cut(s)	20, 383, 971, 1033, 1056, 1122, 1123, 1240, 1548
StyI	CCWWGG	1 cut(s)	1395
TaaI	ACNGT	4 cut(s)	934, 1049, 1204, 1358
TasI	AATT	2 cut(s)	232, 1371
TauI	GCSGC	3 cut(s)	446, 656, 959
TfiI	GAWTC	2 cut(s)	175, 626
Tru1I	TTAA	3 cut(s)	594, 756, 1461
Tru9I	TTAA	3 cut(s)	594, 756, 1461
TscAI	CASTG	3 cut(s)	937, 1281, 1363
TseFI	GTSAC	2 cut(s)	934, 1198
TseI	GCWGC	2 cut(s)	123, 1133
Tsp45I	GTSAC	2 cut(s)	934, 1198
TspDTI	ATGAA	6 cut(s)	519, 1096, 1107, 1329, 1403, 1533
TspMI	CCCGGG	1 cut(s)	1123
TspRI	CASTG	3 cut(s)	937, 1281, 1363
VpaK11BI	GGWCC	2 cut(s)	62, 917
XceI	RCATGY	2 cut(s)	404, 763
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1529
XmaI	CCCGGG	1 cut(s)	1123
XmnI	GAANNNNTTC	1 cut(s)	497
XspI	CTAG	4 cut(s)	740, 768, 921, 977
Zsp2I	ATGCAT	2 cut(s)	1179, 1314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.