FvH4_4g07490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009584
Physical Location & Seq
Reverse (-)
6852827 .. 6859979
7153 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07490.t1

Sequence Viewer

Length: 1227 bp
ATGGCAGCCTCCATGCTTTTCGTCTTCTCCATATTCGCCGCCCTATTATTCACCGCAGTTCCGGCAGAGCCACCGCTTCGAAACGACGGCGCTCCGGAGGTTGCAGATTCGTCGACAAGACTCCAACTCCAACTTCGGTTGCTGGAGACCAAGGTTTACCTTCTAGAATCAAGCATTGATGAAAAAATACGTGAACTGAGGAAGAAGGATGAAAAAATCAGGGAGATGGAAGAGATTATTCAAGGGAAATCAAACAGCAATGCATTTTACCAAAGTGAGACCGAGGCTGTCCAGGGAAGAGAAGATTTAGGTGCTAAGAAAATCACAGGTAAGTTACATGAGAAGAATGCTGAGGAGCTTGTGAATCAGGTTATTGAGGACATCATATTGCAGGATAAATTAAAAGATAAACGGGAAGCCCAAGCAAGGGTAGCTGATCAGAAAACCCAGGAATTGAAATTTAGGGCAGAAGATGCTCATGGAGGATGGCTATCAGTTTTACATTCTGTTGCAGTTCATATAAGCCAGTTTCAGTCATACATAGTGACTTACTGGAATGAATGTGGGAGACCAGCCTTGGATGTGGCAATTGATAAGGCATTGGAAATAAAAGGTCAGGTTAAAAGTTCGGCTCAGCTCAGCATTGAAACAATCAAATATGAATTGATACCAGCTTTGAAGAAACAATCTTTGGAAATTGTAGCCTATCTTGAACCAAATATTCAAGTACTCACTGCAAAAACTGTTGATATCTACCATTCGTCAAAGAGTTCTGTAGTACCATTTGTTTTCAAGGTACAAGAAATGGCAGATCCTCACATTCAGGTAGCTAAGAAGTTAACCAAGCCATACATAGCCCAGATTCTGATGATGATAGATGAGGCTACAATGGTGACAAGAACTCATTTGGCTAACATATACGTTGTGTTGAAGCCATATACAAAACAAGTACTCCCCGCATGCAGAAAATTTATTAAAGCTGCAACATTTTATCATCATAAGGTCCAAAAAATGCTCAAAGACAATAGGTTGACACAATCACTTGCAACTATCGAACTGGCATGGCTTCTGGCCACTGCTTTACTGGCCCTGCCTGCAGTTCTGATGCTTAAGTTGACTTACGTTATTTTCAGAAAAAATGCAAATAATAAGCGTATTCATAGTTCCTATAGATATCATATAGTTCCTACACGCCGCAAGCTCAAGCGTGCTCATCCTGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

46.48

Weight (kDa)

9.43

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 5 cut(s) 39, 54, 74, 957, 1195
AclWI GGATC 1 cut(s) 806
AcoI YGGCCR 1 cut(s) 1071
AcsI RAATTY 2 cut(s) 458, 968
AfaI GTAC 4 cut(s) 729, 780, 798, 951
AfiI CCNNNNNNNGG 2 cut(s) 426, 427
AflII CTTAAG 1 cut(s) 1109
AgsI TTSAA 8 cut(s) 242, 457, 647, 679, 713, 725, 793, 931
AjnI CCWGG 2 cut(s) 291, 447
AjuI GAANNNNNNNTTGG 4 cut(s) 117, 149, 674, 706
AluBI AGCT 7 cut(s) 358, 434, 637, 674, 830, 980, 1201
AluI AGCT 7 cut(s) 358, 434, 637, 674, 830, 980, 1201
Alw21I GWGCWC 1 cut(s) 1213
Alw26I GTCTC 3 cut(s) 140, 272, 562
AlwI GGATC 1 cut(s) 806
AlwNI CAGNNNCTG 1 cut(s) 865
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 2 cut(s) 1071, 1086
ApeKI GCWGC 2 cut(s) 5, 980
ApoI RAATTY 2 cut(s) 458, 968
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 1003, 1087
AsuHPI GGTGA 2 cut(s) 43, 904
AsuII TTCGAA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 1003
BalI TGGCCA 1 cut(s) 1073
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 1213
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 17, 967
BccI CCATC 2 cut(s) 220, 480
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BciT130I CCWGG 2 cut(s) 293, 449
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 3 cut(s) 140, 272, 562
BfaI CTAG 1 cut(s) 164
BfmI CTRYAG 3 cut(s) 774, 1095, 1168
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 1109
BisI GCNGC 4 cut(s) 6, 39, 981, 1195
BlpI GCTNAGC 2 cut(s) 633, 638
BlsI GCNGC 4 cut(s) 7, 40, 982, 1196
BmcAI AGTACT 2 cut(s) 729, 951
Bme1390I CCNGG 2 cut(s) 293, 449
Bme18I GGWCC 1 cut(s) 1003
BmgT120I GGNCC 2 cut(s) 1003, 1087
BmrFI CCNGG 2 cut(s) 293, 449
BmsI GCATC 2 cut(s) 463, 1095
BpiI GAAGAC 1 cut(s) 16
BpmI CTGGAG 1 cut(s) 164
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 2 cut(s) 633, 638
Bpu14I TTCGAA 1 cut(s) 79
BpuEI CTTGAG 1 cut(s) 1187
BsaAI YACGTR 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 490
BsaI GGTCTC 3 cut(s) 140, 272, 562
BsaJI CCNNGG 5 cut(s) 150, 282, 292, 447, 576
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 936, 966
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 427
Bse1I ACTGG 4 cut(s) 526, 557, 1062, 1089
Bse3DI GCAATG 1 cut(s) 265
Bse8I GATNNNNATC 1 cut(s) 490
BseAI TCCGGA 1 cut(s) 94
BseBI CCWGG 2 cut(s) 293, 449
BseDI CCNNGG 5 cut(s) 150, 282, 292, 447, 576
BseGI GGATG 4 cut(s) 214, 491, 586, 1213
BseJI GATNNNNATC 1 cut(s) 490
BseLI CCNNNNNNNGG 2 cut(s) 426, 427
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 4 cut(s) 188, 342, 647, 652
BseNI ACTGG 4 cut(s) 526, 557, 1062, 1089
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 2 cut(s) 17, 967
BshFI GGCC 2 cut(s) 1073, 1088
BsiHKAI GWGCWC 1 cut(s) 1213
BsiSI CCGG 2 cut(s) 62, 95
BslI CCNNNNNNNGG 2 cut(s) 426, 427
BsmAI GTCTC 3 cut(s) 140, 272, 562
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 2 cut(s) 1073, 1088
Bso31I GGTCTC 3 cut(s) 140, 272, 562
Bsp119I TTCGAA 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 1213
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 2 cut(s) 436, 811
Bsp1720I GCTNAGC 2 cut(s) 633, 638
BspACI CCGC 5 cut(s) 39, 54, 74, 957, 1195
BspANI GGCC 2 cut(s) 1073, 1088
BspCNI CTCAG 4 cut(s) 189, 343, 646, 651
BspEI TCCGGA 1 cut(s) 94
BspMAI CTGCAG 1 cut(s) 1099
BspPI GGATC 1 cut(s) 806
BspT104I TTCGAA 1 cut(s) 79
BspTI CTTAAG 1 cut(s) 1109
BspTNI GGTCTC 3 cut(s) 140, 272, 562
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 4 cut(s) 526, 557, 1062, 1089
BssECI CCNNGG 5 cut(s) 150, 282, 292, 447, 576
BssMI GATC 2 cut(s) 436, 811
BssT1I CCWWGG 2 cut(s) 150, 576
Bst2UI CCWGG 2 cut(s) 293, 449
Bst4CI ACNGT 1 cut(s) 745
Bst6I CTCTTC 2 cut(s) 225, 292
BstAFI CTTAAG 1 cut(s) 1109
BstAPI GCANNNNNTGC 1 cut(s) 473
BstBAI YACGTR 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 4 cut(s) 961, 1095, 1199, 1209
BstDEI CTNAG 6 cut(s) 197, 315, 351, 633, 638, 831
BstF5I GGATG 4 cut(s) 214, 491, 586, 1213
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 2 cut(s) 439, 814
BstMAI GTCTC 3 cut(s) 140, 272, 562
BstMBI GATC 2 cut(s) 436, 811
BstMWI GCNNNNNNNGC 5 cut(s) 62, 431, 473, 1085, 1094
BstNI CCWGG 2 cut(s) 293, 449
BstNSI RCATGY 1 cut(s) 963
BstSCI CCNGG 2 cut(s) 291, 447
BstSFI CTRYAG 3 cut(s) 774, 1095, 1168
BstV1I GCAGC 2 cut(s) 17, 967
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BsuRI GGCC 2 cut(s) 1073, 1088
BtsCI GGATG 4 cut(s) 214, 491, 586, 1213
BtsI GCAGTG 2 cut(s) 732, 1074
BtsIMutI CAGTG 2 cut(s) 732, 1074
Cac8I GCNNGC 4 cut(s) 961, 1095, 1199, 1209
CaiI CAGNNNCTG 1 cut(s) 865
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 1003, 1087
Csp6I GTAC 4 cut(s) 728, 779, 797, 950
CviAII CATG 5 cut(s) 13, 338, 479, 960, 1062
CviQI GTAC 4 cut(s) 728, 779, 797, 950
DdeI CTNAG 6 cut(s) 197, 315, 351, 633, 638, 831
DpnI GATC 2 cut(s) 438, 813
DpnII GATC 2 cut(s) 436, 811
EaeI YGGCCR 1 cut(s) 1071
Eam1104I CTCTTC 2 cut(s) 225, 292
EarI CTCTTC 2 cut(s) 225, 292
Eco130I CCWWGG 2 cut(s) 150, 576
Eco31I GGTCTC 3 cut(s) 140, 272, 562
Eco32I GATATC 2 cut(s) 751, 1175
Eco47I GGWCC 1 cut(s) 1003
EcoRII CCWGG 2 cut(s) 291, 447
EcoRV GATATC 2 cut(s) 751, 1175
EcoT14I CCWWGG 2 cut(s) 150, 576
EcoT22I ATGCAT 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 150, 576
FaeI CATG 5 cut(s) 16, 341, 482, 963, 1065
FatI CATG 5 cut(s) 12, 337, 478, 959, 1061
FauI CCCGC 1 cut(s) 964
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 4 cut(s) 6, 39, 981, 1195
FokI GGATG 4 cut(s) 221, 498, 593, 1200
Fsp4HI GCNGC 4 cut(s) 6, 39, 981, 1195
FspBI CTAG 1 cut(s) 164
GlaI GCGC 1 cut(s) 91
GluI GCNGC 4 cut(s) 6, 39, 981, 1195
GsuI CTGGAG 1 cut(s) 164
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 2 cut(s) 1073, 1088
HapII CCGG 2 cut(s) 62, 95
HhaI GCGC 1 cut(s) 92
Hin1II CATG 5 cut(s) 16, 341, 482, 963, 1065
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 4 cut(s) 114, 840, 1032, 1116
HindII GTYRAC 4 cut(s) 114, 840, 1032, 1116
HinfI GANTC 5 cut(s) 107, 120, 167, 364, 862
HpaI GTTAAC 1 cut(s) 840
HpaII CCGG 2 cut(s) 62, 95
HphI GGTGA 2 cut(s) 43, 904
Hpy166II GTNNAC 6 cut(s) 114, 157, 194, 840, 1032, 1116
Hpy188I TCNGA 4 cut(s) 441, 867, 1104, 1133
Hpy188III TCNNGA 4 cut(s) 95, 164, 710, 1217
Hpy8I GTNNAC 6 cut(s) 114, 157, 194, 840, 1032, 1116
Hpy99I CGWCG 2 cut(s) 89, 115
HpyAV CCTTC 2 cut(s) 170, 199
HpyCH4III ACNGT 1 cut(s) 745
HpyCH4IV ACGT 3 cut(s) 190, 921, 1122
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 431, 473, 1085, 1094
HpyF3I CTNAG 6 cut(s) 197, 315, 351, 633, 638, 831
HpySE526I ACGT 3 cut(s) 190, 921, 1122
Hsp92II CATG 5 cut(s) 16, 341, 482, 963, 1065
HspAI GCGC 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 436
KspAI GTTAAC 1 cut(s) 840
Kzo9I GATC 2 cut(s) 436, 811
LmnI GCTCC 2 cut(s) 97, 355
Lsp1109I GCAGC 2 cut(s) 17, 967
LweI GCATC 2 cut(s) 463, 1095
MaeI CTAG 1 cut(s) 164
MaeII ACGT 3 cut(s) 190, 921, 1122
MaeIII GTNAC 3 cut(s) 333, 544, 892
MalI GATC 2 cut(s) 438, 813
MboI GATC 2 cut(s) 436, 811
MboII GAAGA 8 cut(s) 16, 214, 242, 309, 314, 355, 482, 691
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 1 cut(s) 1213
MlsI TGGCCA 1 cut(s) 1073
MluCI AATT 7 cut(s) 398, 452, 458, 588, 662, 696, 968
MluNI TGGCCA 1 cut(s) 1073
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 148, 154
MnlI CCTC 9 cut(s) 19, 91, 192, 277, 346, 370, 476, 825, 874
Mox20I TGGCCA 1 cut(s) 1073
Mph1103I ATGCAT 1 cut(s) 265
MroI TCCGGA 1 cut(s) 94
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 5 cut(s) 401, 621, 839, 975, 1110
Msp20I TGGCCA 1 cut(s) 1073
MspCI CTTAAG 1 cut(s) 1109
MspI CCGG 2 cut(s) 62, 95
MspR9I CCNGG 2 cut(s) 293, 449
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 2 cut(s) 293, 449
MwoI GCNNNNNNNGC 5 cut(s) 62, 431, 473, 1085, 1094
NdeII GATC 2 cut(s) 436, 811
NlaIII CATG 5 cut(s) 16, 341, 482, 963, 1065
NmuCI GTSAC 2 cut(s) 544, 892
NsiI ATGCAT 1 cut(s) 265
NspI RCATGY 1 cut(s) 963
NspV TTCGAA 1 cut(s) 79
PaeI GCATGC 1 cut(s) 963
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 4 cut(s) 107, 167, 364, 862
PkrI GCNGC 4 cut(s) 7, 40, 982, 1196
PleI GAGTC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 114
Ppu21I YACGTR 1 cut(s) 191
Psp6I CCWGG 2 cut(s) 291, 447
PspGI CCWGG 2 cut(s) 291, 447
PspPI GGNCC 2 cut(s) 1003, 1087
PsrI GAACNNNNNNTAC 2 cut(s) 1147, 1179
PstI CTGCAG 1 cut(s) 1099
PstNI CAGNNNCTG 1 cut(s) 865
PsuI RGATCY 1 cut(s) 811
RsaI GTAC 4 cut(s) 729, 780, 798, 951
RsaNI GTAC 4 cut(s) 728, 779, 797, 950
SalI GTCGAC 1 cut(s) 112
SaqAI TTAA 5 cut(s) 401, 621, 839, 975, 1110
SatI GCNGC 4 cut(s) 6, 39, 981, 1195
Sau3AI GATC 2 cut(s) 436, 811
Sau96I GGNCC 2 cut(s) 1003, 1087
ScaI AGTACT 2 cut(s) 729, 951
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 2 cut(s) 293, 449
SduI GDGCHC 1 cut(s) 1213
SfaNI GCATC 2 cut(s) 463, 1095
SfcI CTRYAG 3 cut(s) 774, 1095, 1168
SfuI TTCGAA 1 cut(s) 79
SinI GGWCC 1 cut(s) 1003
SmlI CTYRAG 2 cut(s) 1109, 1202
SmoI CTYRAG 2 cut(s) 1109, 1202
SphI GCATGC 1 cut(s) 963
Sse9I AATT 7 cut(s) 398, 452, 458, 588, 662, 696, 968
SsiI CCGC 5 cut(s) 39, 54, 74, 957, 1195
SspI AATATT 1 cut(s) 721
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 291, 447
StyI CCWWGG 2 cut(s) 150, 576
TaaI ACNGT 1 cut(s) 745
TaiI ACGT 3 cut(s) 193, 924, 1125
TaqI TCGA 3 cut(s) 79, 113, 1053
TaqII GACCGA 1 cut(s) 296
TasI AATT 7 cut(s) 398, 452, 458, 588, 662, 696, 968
TatI WGTACW 2 cut(s) 727, 949
TauI GCSGC 2 cut(s) 41, 1197
TfiI GAWTC 4 cut(s) 107, 167, 364, 862
Tru1I TTAA 5 cut(s) 401, 621, 839, 975, 1110
Tru9I TTAA 5 cut(s) 401, 621, 839, 975, 1110
TscAI CASTG 2 cut(s) 739, 1081
TseFI GTSAC 2 cut(s) 544, 892
TseI GCWGC 2 cut(s) 5, 980
Tsp45I GTSAC 2 cut(s) 544, 892
TspDTI ATGAA 6 cut(s) 195, 225, 506, 573, 675, 1148
TspRI CASTG 2 cut(s) 739, 1081
Vha464I CTTAAG 1 cut(s) 1109
VpaK11BI GGWCC 1 cut(s) 1003
XapI RAATTY 2 cut(s) 458, 968
XbaI TCTAGA 1 cut(s) 163
XceI RCATGY 1 cut(s) 963
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 1 cut(s) 164
ZrmI AGTACT 2 cut(s) 729, 951
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.